CN102888366A - Compound bacteria and method for preparing textile fiber using same - Google Patents
Compound bacteria and method for preparing textile fiber using same Download PDFInfo
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- CN102888366A CN102888366A CN2012103421643A CN201210342164A CN102888366A CN 102888366 A CN102888366 A CN 102888366A CN 2012103421643 A CN2012103421643 A CN 2012103421643A CN 201210342164 A CN201210342164 A CN 201210342164A CN 102888366 A CN102888366 A CN 102888366A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention relates to compound bacteria and a method for preparing textile fiber using the same. The compound bacteria comprise bacillus stored with a number of CGMCC No. 5971, rheinheimera tangshanensis stored with a number of CGMCC No. 5972, pseudomonas fluorescens stored with a number of CGMCC No. 5974, and Wickerhamomyces anomalus stored with a number of CGMCC No. 5975. The method comprises the following steps of: preparing bacteria liquid; processing raw materials; and preparing fibers, wherein fiber preparing steps comprise the following steps of: degrading, biologically degrading, sterilizing through steam, and obtaining the fibers; drying; and carding. The method provided by the invention is environment-friendly; waste liquid is directly transformed into organic fertilizer, and therefore, zero emission and zero pollution are achieved. A biological treating process performs protection to the fibers. Compared with a traditional chemical method, the method provided by the invention has the advantages of being low in producing cost, high in economic benefit, and is capable of saving an energy resource and protecting an environment.
Description
Technical field
The present invention relates to a kind of textile fibres, relate in particular to a kind of composite flora and prepare the method for textile fibres with it.
Background technology
In recent years, day by day exhausted along with the petrochemical industry resource, bast-fibre enjoys doting on of human consumer because of having the good characteristics such as ecology, environmental protection, and its demand is at increase year after year, global natural fiber speedup 8% in every year.The maximum characteristics of bast-fibre raw material are that fibre content is high, and elongated being conducive to of fiber interweaves, and intensity is good; The fiber cell is little, cell wall is thick, wall chamber ratio is large; Because the bast-fibre lumen is thin, fiber trivial matter opaqueness is high.But its shortcoming is fiber is not easy wire-dividing broom purification, makes the fabric air-permeability degree of making low.
From numb raw material, prepare fiber in the prior art, how by chemical process, the waste liquor contamination sublimity that produces during chemical process is produced, damage to land, polluted air, and also power consumption is high, and power consumption and water consumption are large.Do not meet national energy-saving and reduce discharging policy.Material can not reach the Efficient Cycle recycling.Chemicals can't separate from waste liquid, and organism and chemicals mix, and organism also can't be reused, and causes a large amount of losses.
Therefore, be necessary to develop biological fibre technology processed, fundamentally solve an above-mentioned pollution difficult problem, energy-saving and emission-reduction, water-saving reduces production costs and improves the rate of utilization of material.
Summary of the invention
The objective of the invention is to overcome the defects of the chemical legal system fiber of prior art, a kind of new preparation fiber biological bacteria is provided.The contriver has obtained to be suitable for preparing the biological bacteria that can realize the foregoing invention purpose of fiber by long screening operation.
Particularly, the invention provides a kind of composite flora, it comprises the genus bacillus that preserving number is CGMCC No.5971 (Bacillus sp.), preserving number is the silent Salmonella (Rheinheimera tangshanensis) of the Tangshan Lay mattress of CGMCC No.5972, preserving number is the Pseudomonas fluorescens (Pseudomonas fluorescens) of CGMCC No.5974, preserving number be this Wickerhamomyces anomalus bacterium of Wickerhamomyces anomalus(of CGMCC No.5975 in malt juice liquid medium 25 ℃ cultivated three days, cell is spherical, avette, sausage shape, size is (4.8-14.4) * (3.6-7.2) μ m, has precipitation to form.25 ℃ of cultivations in wort agar inclined-plane one month, bacterium colony cheese shape, oyster white, surface smoothing, not reflective, edge root shape.Corn Meal Agar Dalmau is dull and stereotyped to be cultivated, and produces pseudohypha).
Described composite flora can be used in the preparation textile fibres.
The present invention also provides a kind of method for preparing textile fibres, and it mainly comprises step:
1) configuration of bacterium liquid: above-mentioned composite flora is configured to the composite bacteria aqueous solution according to following mass ratio, is bacterium liquid:
Genus bacillus: the Tangshan Lay mattress Salmonella of writing from memory: Pseudomonas fluorescens: Wickerhamomyces anomalus is 2-3:1-2:1-2:2-3;
2) raw material is processed: the crudefiber crop raw material is cut into chunks, and raw material is put into the fermentation vat swollen;
3) preparation of fiber, it comprises step:
Biological degradation: the raw material soaking after will discongesting is in the bacterium liquid that configures;
Wet sterilization: with the raw material after the above-mentioned biological degradation from bacterium liquid, pull out, draining, pass into water vapor sterilization;
Obtaining of fiber: the raw material after will sterilizing carries out one section corase grind, becomes fibrous bundle; Above-mentioned one section corase grind is carried out two sections fine grindings, make fibrous bundle disperse into single fiber; Screening, filter through the fibrous bundle in the slurries after one section corase grind and the two sections fine grindings, defibrination makes it make single fiber again;
Dry, combing: the above-mentioned fiber that makes is soaked in warm water, and then oven dry, combing is for the preparation of textile fibres.
The density of the bacterium liquid that wherein, forms in the step 1) is 6 * 10
7Individual/more than the ml bacterium.
Wherein, step 2) in, described crudefiber crop raw material is flax, ramie, yellow bluish dogbane or sisal hemp.The described swollen time is 10-12h.
Wherein, the described biological degradation temperature of step 3) remains on 35-40 ℃, time 30-34 hour.The mass ratio of the raw material after discongesting and bacterium liquid is 1:7-9.Described steam sterilizing is water at atmospheric pressure steam sterilizing 10-30 minute.
Further, the soak solution step 2) behind the immersion raw material is through flocculating, precipitate, supernatant liquor recycling, throw out input methane-generating pit fermentation generation biogas.
The advantage that biological method provided by the invention prepares fiber is: 1) free from environmental pollution: waste liquid directly changes into organic fertilizer, reaches zero release, and zero pollutes.2) biological method can play a protective role to fiber, compares with traditional chemical process, and present method can all reclaim full fiber and half fiber, has therefore improved yield.3) biological method is degraded under normal pressure, energy-conservation, reduction of discharging, low-carbon (LC).4) production cost is low, and economic benefit is high.
Byproduct of the present invention is transported to settling tank flocculation, precipitation, and supernatant liquor returns second stage employ, remakes presoak and uses.Contain the nutrient for plants such as abundant gas chromatography and N, P, K in the throw out, throw out mixes with old bacterium liquid (the raw material thickness bacterium liquid of repeatedly degrading also contains N, P, K, Fe and trace element) again, acidifying, then together enter in the marsh gas fermentation pool, produce biogas.With the Boiler Ash mixing granulation of natural pond slag, natural pond liquid and pulverizing, the granulation fertilizer dispatches from the factory at last, realizes zero release.
The biological bacteria of the present invention by providing above-mentioned contriver to obtain through long creation work, the further perfect cellulosic technology of existing preparation, this technology reduced the reaction times, improved the purity and the yield that make fiber, so that can be applied in actual production on a large scale.Applying biological bacterium of the present invention at short notice degrading plant obtains fiber, and the byproduct twice transformation becomes biogas, and biogas coal supply, gas boiler for dual purpose combustion heating are saved coal consumption.At last, the natural pond slag is made organic fertilizer, has formed the economic circulation new model of " material organic transformation ", reaches the no waste discharging, namely zero release.Fundamentally solved the pollution difficult problem of chemical preparation fiber of the prior art.Energy-saving and emission-reduction, water-saving has reduced production cost, has improved the rate of utilization of material.
For above and other objects of the present invention, feature and advantage can be become apparent, preferred embodiment cited below particularly, and cooperation accompanying drawing are described in detail below.
Description of drawings
Fig. 1 is the schema according to the preparation textile fibres of one embodiment of the invention.
Embodiment
The configuration of embodiment 1 bacterium liquid
The biological bacteria that adopts among the present invention on April 6th, 2012 at the common micro-organisms center (CGMCC of China Committee for Culture Collection of Microorganisms, No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City) preservation, it comprises the genus bacillus that preserving number is CGMCC No.5971 (Bacillus sp.), preserving number is the silent Salmonella (Rheinheimera tangshanensis) of the Tangshan Lay mattress of CGMCC No.5972, preserving number is the Pseudomonas fluorescens (Pseudomonas fluorescens) of CGMCC No.5974, preserving number is the Wickerhamomyces anomalus of CGMCC No.5975.
Above-mentioned composite flora is configured to the composite bacteria aqueous solution according to following mass ratio, is bacterium liquid:
Genus bacillus: the Tangshan Lay mattress Salmonella of writing from memory: Pseudomonas fluorescens: Wickerhamomyces anomalus is 2-3:1-2:1-2:2-3; The density of the bacterium liquid that forms is 6 * 10
7Individual/more than the ml bacterium, for subsequent use.
Embodiment 2 prepares fiber from flax
Take flax as raw material, specify the preparation method of fiber.Composite flora is configured according to following mass ratio:
Genus bacillus: the Tangshan Lay mattress Salmonella of writing from memory: Pseudomonas fluorescens: Wickerhamomyces anomalus is 3:1:2:3.
Specifically please refer to Fig. 1, the preparation method's of fiber flow process is divided into three phases: preparatory stage, fiber workshop section and byproduct workshop section.
(1) preparatory stage: 1,4
Flax that harvesting is returned immerse soak in storehouse or the fermentation vat wash, cold soaking, at first with foreign material flush awaies such as raw material appearance earth, soak simultaneously, water temperature is natural temperature, the time with soak into, swollen is as the criterion 10-12 hour.After the liquid muddiness after repeatedly soaking, supernatant liquor can also reuse after flocculating, precipitating.Biogas is produced in the fermentation of throw out input methane-generating pit.
(2) fiber workshop section: 6-12
6) biological degradation: in the raw material input biological bacteria degraded storehouse or tank after will discongesting, be immersed in the bacterium liquid of embodiment 1 preparation, the mass ratio of the raw material after discongesting and bacterium liquid is 1:8, and temperature remains on 35-40 ℃, time 30-34 hour.Under the condition of biological bacteria DeR occuring, brings into play its single-minded effect.
7) wet sterilization: after raw materials pretreatment is finished, with raw material from bacterium liquid, pull out, draining, be input in the steam storehouse, pass into water at atmospheric pressure steam 10-30 minute sterilization.Paste roller mill is inputted with raw material in the lower end.
8) obtaining of fiber: the raw material after will sterilizing carries out one section corase grind, becomes fibrous bundle.
9) above-mentioned one section corase grind is carried out two sections fine grindings, make fibrous bundle disperse into single fiber.
10) multiple sieve: screening, filter through the fibrous bundle in the slurries after one section corase grind and the two sections fine grindings, defibrination makes it make single fiber again.
11) dry, combing: the above-mentioned fiber that makes is soaked in warm water, then oven dry, combing, drawing-off, it is parallel that fiber is further stretched.
12) preparation textile fibres: adopt further Technology to prepare textile fibres the fiber of above-mentioned acquisition.
(3) byproduct stage: A-C
Isolated raw material residuum contains abundant nutritive ingredient among the A, 3, and fermentation changes into ox, sheep feed.
After the liquid muddiness through repeatedly soaking, washing among the B, 4, through flocculation, precipitation, the supernatant liquor recycling, the fermentation of throw out input methane-generating pit produces biogas, passes into the coal gas boiler for dual purpose and does fuel, can play effect of energy.
C, biological organic fertilizer: natural pond slag, natural pond liquid after the methane-generating pit fermentation are the biological organic fertilizers that enriches, and liquid topdresses as farm crop and flower nutrient solution uses, and the solid granulation is made base manure and used, and all is green fertilizers.
The physical performance index measurement result of the fiber that obtains please see table 1 for details.
Embodiment 3 prepares Mierocrystalline cellulose from ramie
Concrete preparation technology is with embodiment 2.Difference is that composite flora is configured according to following mass ratio:
Genus bacillus: the Tangshan Lay mattress Salmonella of writing from memory: Pseudomonas fluorescens: Wickerhamomycesanomalus is 3:1:2:2; During biological degradation, the mass ratio of the raw material after discongesting and bacterium liquid is 1:7.
The physical performance index measurement result of the fiber that obtains please see table 1 for details.
Embodiment 4 prepares Mierocrystalline cellulose from yellow bluish dogbane
Concrete preparation technology is with embodiment 2.Difference is that composite flora is configured according to following mass ratio:
Genus bacillus: the Tangshan Lay mattress Salmonella of writing from memory: Pseudomonas fluorescens: Wickerhamomyces anomalus is 3:2:2:2; During biological degradation, the mass ratio of the raw material after discongesting and bacterium liquid is 1:8.5.
The physical performance index measurement result of the fiber that obtains please see table 1 for details.
Embodiment 5 prepares Mierocrystalline cellulose from sisal hemp
Concrete preparation technology is with embodiment 2.Difference is that composite flora is configured according to following mass ratio:
Genus bacillus: the Tangshan Lay mattress Salmonella of writing from memory: Pseudomonas fluorescens: Wickerhamomyces anomalus is 2:1:2:3; During biological degradation, the mass ratio of the raw material after discongesting and bacterium liquid is 1:9.
The physical performance index measurement result of the fiber that obtains please see table 1 for details.
Table 1
Although the present invention discloses as above with preferred embodiment; so it is not to limit the present invention; any person of ordinary skill in the field; without departing from the spirit and scope of the present invention; when can doing a little change and improvement, so protection scope of the present invention is as the criterion when looking the claim person of defining.
Claims (10)
1. composite flora, it comprises the genus bacillus that preserving number is CGMCC No.5971 (Bacillus sp.), preserving number is that the silent Salmonella (Rheinheimera tangshanensis) of Tangshan Lay mattress of CGMCC No.5972, Pseudomonas fluorescens (Pseudomonas fluorescens), the preserving number that preserving number is CGMCC No.5974 are the Wickerhamomyces anomalus of CGMCC No.5975.
2. the application of composite flora claimed in claim 1 in the preparation textile fibres.
3. a method for preparing textile fibres is characterized in that, comprises step:
1) configuration of bacterium liquid: composite flora claimed in claim 1 is configured to the composite bacteria aqueous solution according to following mass ratio, is bacterium liquid:
Genus bacillus: the Tangshan Lay mattress Salmonella of writing from memory: Pseudomonas fluorescens: Wickerhamomyces anomalus is 2-3:1-2:1-2:2-3;
2) raw material is processed: the crudefiber crop raw material is cut into chunks, and raw material is put into the fermentation vat swollen;
3) preparation of fiber, it comprises step:
Biological degradation: the raw material soaking after will discongesting is in the bacterium liquid that configures;
Wet sterilization: with the raw material after the above-mentioned biological degradation from bacterium liquid, pull out, draining, pass into water vapor sterilization;
Obtaining of fiber: the raw material after will sterilizing carries out one section corase grind, becomes fibrous bundle; Above-mentioned one section corase grind is carried out two sections fine grindings, make fibrous bundle disperse into single fiber; Screening, filter through the fibrous bundle in the slurries after one section corase grind and the two sections fine grindings, defibrination makes it make single fiber again;
Dry, combing: the above-mentioned fiber that makes is soaked in warm water, and then oven dry, combing is for the preparation of textile fibres.
4. method according to claim 3 is characterized in that, the density of the bacterium liquid that forms in the step 1) is 6.0 * 10
7Individual/more than the ml bacterium.
5. method according to claim 3 is characterized in that step 2) in, described former crudefiber crop is flax, ramie, yellow bluish dogbane or sisal hemp.
6. according to claim 3 or 5 described methods, it is characterized in that step 2) the described swollen time is 10-12h.
7. method according to claim 3 is characterized in that, the described biological degradation temperature of step 3) remains on 35-40 ℃, time 30-34 hour.
8. method according to claim 3 is characterized in that, in the described biological degradation of step 3), the mass ratio of the raw material after discongesting and bacterium liquid is 1:7-9.
9. method according to claim 3 is characterized in that, the described steam sterilizing of step 3) is water at atmospheric pressure steam sterilizing 10-30 minute.
10. method according to claim 3 is characterized in that step 2) in soak soak solution behind the raw material through flocculation, precipitation, the supernatant liquor recycling, the fermentation of throw out input methane-generating pit produces biogas.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210342164.3A CN102888366B (en) | 2012-09-17 | 2012-09-17 | A kind of composite flora and prepare the method for textile fibres with it |
| PCT/CN2013/001086 WO2014040385A1 (en) | 2012-09-17 | 2013-09-16 | Complex microbial flora and application thereof |
| US14/428,967 US20150233051A1 (en) | 2012-09-17 | 2013-09-16 | Pseudomonas fluorescens cgmcc#5974 and its application thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014040385A1 (en) * | 2012-09-17 | 2014-03-20 | 北京天安生物科技有限公司 | Complex microbial flora and application thereof |
| CN105624800A (en) * | 2014-11-04 | 2016-06-01 | 华中科技大学 | Biological bleaching method for production of refined dried ramie |
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| CN101838613A (en) * | 2009-09-30 | 2010-09-22 | 河南省恒隆态生物工程股份有限公司 | Agricultural straw fast-degradation decomposition agent strain composition |
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| CN102174399A (en) * | 2010-12-31 | 2011-09-07 | 广西赛尔纸业有限公司 | Method for preparing herbal biological fiber and biological paper pulp |
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| CN102888366B (en) | 2015-11-25 |
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