CN102835387A - Method for preparing sealed glass slide for microscope - Google Patents

Abstract

The invention discloses a method for preparing a sealed glass slide for a microscope and relates to the field of microscope specimen preparing. According to the invention, cedar wood oil which is commonly used in the process of microscope oil-immersion microscopy is smeared around a cover glass, the cover glass is sealed, and the slide specimen is kept undried for a long time, so that the specimen is preserved and observed for a longer time. The method takes advantage of the cedar wood oil which has good sealing performance, does not dissolve in water and has no effect on the specimen, thus the disadvantage that the specimen prepared by ordinary methods dries in a short time and can not be preserved and observed for a long time is overcome. The invention provides a simple and effective method which prolongs the preservation and observation time of the microscope specimen.

Description

A kind of preparation method of microscopical sealing slide sample

Technical field

The invention provides the long-time preparation method of preserving and observing of a kind of microslide sample, relate to microscope preparation of specimen field.

Background technology

In ambits such as medical science, agronomy and biology, microscope is a kind of comparatively commonly used and important experimental instrument, is widely used in the every field of modern science and technology at present, is the scientific research and the indispensable aids of imparting knowledge to students such as medical science, biology.In the microscope use, the making of slide sample is absolutely necessary.But the slide sample of conventional method making at present will be killed in the short period (being generally less than 1 hour), can not preserve for a long time and observe.The present invention uses cedar oil to be applied in around the cover glass, and sample is sealed, and prevents the evaporation of sample moisture, thereby can preserve for a long time and observation sample (also not killing in two months with the oil-tightening sample of arborvitae).The microscope oil of using always when cedar oil is microscope oil sem observation, microscope generally is equipped with cedar oil.In addition, cedar oil has good sealing, and is water insoluble, sample do not impacted.

Summary of the invention

The preparation method that the purpose of this invention is to provide a kind of microscopical sealing slide sample overcomes the shortcoming that present microslide sample can not be preserved for a long time and observe.

Technical scheme of the present invention: a kind of preparation method of microscopical sealing slide sample, step is following:

A, the sample of choosing observation for a long time and preserving;

B, according to the requirement of different samples, before the sampling sample is shaken up, draw sample with liquid-transfering gun, then sample is placed on the center of slide and with cover glass building gently, makes the slide sample;

C, be applied in around the cover glass with cedar oil, slide sample good seal;

D, being placed on the microscopical objective table with the slide sample level of good seal;

E, unlatching microscope, the brightness of adjustment microscope is adjusted to focus state with microscope, and sample is observed.

The preparation method of described microscopical sealing slide sample, employed slide is of a size of 76.2 * 25.4 * 1mm, and the lid fragmentation is of a size of 22 * 22 * 0.17mm.

Beneficial effect of the present invention: the inventive method is utilized existing resources and lab material; The simple and effective purpose that has realized long-time preservation and viewing microscope slide sample, that the inventive method has is simple to operate, easy master, advantage such as simple.

Embodiment

Phytoplankton sample to gather from Taihu Lake is an example, as follows operation:

A, phytoplankton sample and the related experiment material choosing and need to be ready to observe;

Before b, the sampling sample is shaken up, draw 0.1 mL/L phytoplankton sample, then sample is placed on the center of slide and with cover glass building gently, makes the slide sample with liquid-transfering gun;

C, with cedar oil being applied in around the cover glass gently, noting when smearing can not the removable cover slide, with slide sample good seal;

D, earlier OLYMPUS CX31 micro objective is transferred to low power lens, with being placed on the microscopical objective table of the slide sample level of good seal;

E, unlatching microscope, microscope coarse adjustment and fine setting are regulated in the brightness of adjustment microscope, and microscope is adjusted to focus state, and sample is observed.

Claims (2)

1. the preparation method of a microscopical sealing slide sample, it is characterized in that: step is following:

A, the sample of choosing observation for a long time and preserving;

B, according to the requirement of different samples, before the sampling sample is shaken up, draw sample with liquid-transfering gun, then sample is placed on the center of slide and with cover glass building gently, makes the slide sample;

C, be applied in around the cover glass with cedar oil, slide sample good seal;

D, being placed on the microscopical objective table with the slide sample level of good seal;

E, unlatching microscope, the brightness of adjustment microscope is adjusted to focus state with microscope, and sample is observed.

2. the preparation method of microscopical sealing slide sample according to claim 1 is characterized in that: employed slide is of a size of 76.2 * 25.4 * 1mm, and the lid fragmentation is of a size of 22 * 22 * 0.17mm.