CN110320079A - A kind of rhizopus slide sample and preparation method thereof - Google Patents
A kind of rhizopus slide sample and preparation method thereof Download PDFInfo
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- CN110320079A CN110320079A CN201910637380.2A CN201910637380A CN110320079A CN 110320079 A CN110320079 A CN 110320079A CN 201910637380 A CN201910637380 A CN 201910637380A CN 110320079 A CN110320079 A CN 110320079A
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- rhizopus
- pearl cotton
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- slide sample
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- 241000235527 Rhizopus Species 0.000 title claims abstract description 92
- 238000002360 preparation method Methods 0.000 title abstract description 6
- 229920000742 Cotton Polymers 0.000 claims abstract description 94
- 238000004519 manufacturing process Methods 0.000 claims abstract description 41
- 238000000034 method Methods 0.000 claims abstract description 15
- 239000001963 growth medium Substances 0.000 claims abstract description 11
- 241000894006 Bacteria Species 0.000 claims abstract description 8
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 24
- 239000003795 chemical substances by application Substances 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 12
- 238000007789 sealing Methods 0.000 claims description 12
- 239000011521 glass Substances 0.000 claims description 10
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 8
- 239000012153 distilled water Substances 0.000 claims description 8
- 235000010489 acacia gum Nutrition 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 7
- 229920000084 Gum arabic Polymers 0.000 claims description 6
- 239000000205 acacia gum Substances 0.000 claims description 6
- 238000010521 absorption reaction Methods 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
- 238000011084 recovery Methods 0.000 claims description 5
- 230000008602 contraction Effects 0.000 claims description 4
- 239000004744 fabric Substances 0.000 claims 2
- 241000978776 Senegalia senegal Species 0.000 claims 1
- 229920001206 natural gum Polymers 0.000 claims 1
- 244000215068 Acacia senegal Species 0.000 description 5
- 210000003128 head Anatomy 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- RNFNDJAIBTYOQL-UHFFFAOYSA-N chloral hydrate Chemical compound OC(O)C(Cl)(Cl)Cl RNFNDJAIBTYOQL-UHFFFAOYSA-N 0.000 description 2
- 229960002327 chloral hydrate Drugs 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 208000005189 Embolism Diseases 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000223259 Trichoderma Species 0.000 description 1
- 206010061418 Zygomycosis Diseases 0.000 description 1
- 239000001785 acacia senegal l. willd gum Substances 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 210000005013 brain tissue Anatomy 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 230000002490 cerebral effect Effects 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 239000006059 cover glass Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 235000021186 dishes Nutrition 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000008236 heating water Substances 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 201000007524 mucormycosis Diseases 0.000 description 1
- 210000002850 nasal mucosa Anatomy 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- CHWRSCGUEQEHOH-UHFFFAOYSA-N potassium oxide Chemical compound [O-2].[K+].[K+] CHWRSCGUEQEHOH-UHFFFAOYSA-N 0.000 description 1
- 229910001950 potassium oxide Inorganic materials 0.000 description 1
- 235000019991 rice wine Nutrition 0.000 description 1
- 239000005060 rubber Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G09—EDUCATION; CRYPTOGRAPHY; DISPLAY; ADVERTISING; SEALS
- G09B—EDUCATIONAL OR DEMONSTRATION APPLIANCES; APPLIANCES FOR TEACHING, OR COMMUNICATING WITH, THE BLIND, DEAF OR MUTE; MODELS; PLANETARIA; GLOBES; MAPS; DIAGRAMS
- G09B23/00—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/305—Fixative compositions
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- Engineering & Computer Science (AREA)
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Abstract
The present invention relates to a kind of production methods of biological slide sample, disclose a kind of rhizopus slide sample and preparation method thereof, the production method of the rhizopus slide sample is, culture medium is placed in the bottom at the first culture dish ware bottom, rhizopus is seeded in the first culture dish, then it is cultivated under conditions of 25 DEG C ± 1 DEG C, form cotton-shaped bacterium colony, pearl cotton is placed again around the first culture dish, the mycelia of rhizopus spread on pearl cotton and growth, then selected from pearl cotton and be distributed sparse rhizopus rhizoid and sporangium production slide sample.The present invention also protects the rhizopus slide sample as made from the production method of rhizopus slide sample.The production method of the rhizopus slide sample of offer of the invention, can be with sporangium visible in detail and rhizoid, and method is simple and easy, and success rate is high.
Description
Technical field
The present invention relates to a kind of production method of biological slide sample, more particularly, to a kind of rhizopus slide sample and its
Production method.
Background technique
Rhizopus is widely distributed, usually harmless to humans, eats sweet yeast for brewing rice wine and saccharified pig feed is exactly the root for selecting saccharifying power strong
Trichoderma strain preparation, but since its distribution is wide, it often can lead to laboratory pollution.Also it can cause food moulding, be a kind of condition cause
Germ can cause mucormycosis, and patient is often accompanied by diabetes, acid poisoning, be easy intrusion schneiderian membrane and submucous tissue, cause bee
Nest tissue is scorching.Also brain tissue can be invaded, cerebral lesion is caused.Blood vessel is invaded, embolism etc. is caused.Therefore rhizopus is also weight in teaching
The object that must be observed in a kind of zygomycete of point explanation and mycology and Microbiology Experiment.
Traditional rhizopus is on Sabouraud glucose agar, incubated at room temperature, and growth is rapid, white mycelium, with the passing of time
Become grey or brown-black, is in cotton sample bacterium colony, is often full of slant tube or plate, microscopy mycelia is thicker, does not separate or seldom
Separate.Rhizoid is formed on stolon, bears sporangiophore with rhizoid relative direction, top forms sporangium.Sporangium is spherical shape,
It is just white, after become black, it is interior full of spherical or oval cystospore.So the characteristics of head mold important identification be sporangium and
Rhizoid, however the rhizopus slide that we buy in experimental teaching, since rhizopus cultural method is often with liquid culture, in order to
Ensure to supply oxygen sufficiently, need to stir tank body or shaking flask culture, thallus can be made to be damaged (sporangium and rhizoid can fall off) in this way,
So the rhizopus slide directly bought is difficult to observe target signature, therefore, it is proposed that a kind of rhizopus slide sample
Production method.
Summary of the invention
For overcome the deficiencies in the prior art, the object of the present invention is to provide a kind of rhizopus slide sample and its production sides
Method, can be with sporangium visible in detail and rhizoid, and method is simple and easy, and success rate is high.
The object of the present invention is to provide a kind of production methods of rhizopus slide sample, in the bottom at the first culture dish ware bottom
Culture medium is placed, rhizopus is seeded in the first culture dish bottom center, is then cultivated under conditions of 25 DEG C ± 1 DEG C, is formed
Cotton-shaped bacterium colony, then place pearl cotton around the first culture dish, rhizoid and the sporangium sprawling of rhizopus and grow into treasure
On pearl cotton, is then selected from pearl cotton and be distributed sparse rhizopus rhizoid and sporangium production slide sample.
Preferably, the production method of above-mentioned rhizopus slide sample, the first culture dish is placed on pearl cotton, and is guaranteed
First culture dish bottom periphery retains pearl cotton.
Preferably, the production method of above-mentioned rhizopus slide sample, when placing pearl cotton around the first culture dish, by the
The related pearl cotton of one culture dish is placed in the second culture dish, and the diameter and height of the first culture dish are respectively less than the second culture dish,
And first culture dish without ware lid, the second culture dish band ware lid.
It is highly preferred that the production method of above-mentioned rhizopus slide sample, precious when placing pearl cotton around the first culture dish
Pearl cotton is round, and the diameter of pearl cotton is equal to the interior diameter of the second culture dish, pearl cotton is laid in the second culture dish, so
Upper part places the first culture dish afterwards.
Preferably, when placing pearl cotton around the first culture dish, pearl cotton is round, and the diameter of pearl cotton is equal to the
The bottom of pearl cotton has been offered a slot or through-hole, has then inlayed the first culture dish ware bottom by the interior diameter of two culture dishes
In the slot or through-hole.
Preferably, the production method of above-mentioned rhizopus slide sample is selected from pearl cotton and is distributed sparse rhizopus vacation
The method of root and sporangium production slide sample are as follows:
It is selected from pearl cotton and is distributed that sparse rhizopus is careful to be placed on glass slide, 1 drop volume concentration 80% of drop~
Rhizopus on 95% alcohol dampening glass slide, it is 2%~3% hydrogen-oxygen that 1 drop mass concentration is added when alcohol does not volatilize completely
Change potassium solution, correct the contraction of alcohol, material is made to restore to the original state, then suck extra potassium hydroxide solution with blotting paper,
Add 1 drop of liquid sealing agent, cover cover plate, suck extra sealing agent, is i.e. production obtains complete rhizopus slide sample.
It is highly preferred that selecting the method for being distributed sparse rhizopus from pearl cotton are as follows: select and spread over from pearl cotton
The rhizopus of the visible single stain of periphery naked eyes.
Preferably, the sealing agent is that gum arabic, distilled water, glycerol, chloraldurate are mixed at normal temperature
Be equipped, wherein gum arabic, distilled water, glycerol, chloraldurate ratio be 3g:5mL:2mL:20g.
Preferably, the pearl cotton selects pure white pearl cotton.
It is highly preferred that the density of the pearl cotton is 0.025~0.035g/cm3, compressive strength is 3.0~4.0kg/cm3,
Water absorption rate is 0.01~0.015kg/cm3, recovery rate is greater than 98%.
Preferably, the culture medium is PDA culture medium.
Second purpose of the invention is to provide a kind of rhizopus slide sample, is using rhizopus slide sample described above
Production method be made.
Compared with prior art, a kind of rhizopus slide sample of the present invention and preparation method thereof, has the advantages that
In order to find that selecting rhizopus step is to cause in routine experiment with sporangium visible in detail and rhizoid
The incomplete principal element of rhizopus, the present invention start with to imagine from the material of rhizopus growth, and exploration is easier to choose
Choosing and the material for not destroying rhizopus complete structure, the present inventor attempted with transparent plastic, rubber, wood, glass
Etc. materials, eventually pass through experiment discovery selected from pearl cotton rhizopus production slide sample have very high success rate.By
Have in pearl cotton fabulous rhizopus the condition of raw growth, easily adhere to, suitable rhizopus is crawled the characteristic of sprawling, and pearl
The elasticity of compression, recovery rate, the characteristic of water absorption rate of cotton will not make the integrality of rhizopus when especially fitting in picking rhizopus
At destruction, therefore, prepared rhizopus slide sample can be readily apparent that the main diagnostic characteristics of sporangium and rhizoid,
The form of head mold is observed, effect is fine.
Detailed description of the invention
Fig. 1 be 2 head mold of the embodiment of the present invention mycelia sprawling encounter pearl cotton and raw growth schematic diagram；
Fig. 2 is the vertical section figure of culture dish in Fig. 1.
Description of symbols: 1, the first culture dish；2, the second culture dish；3, pearl cotton；4, culture medium；5, rhizopus is cotton-shaped
Bacterium colony；6, sprawling encounter pearl cotton and the rhizopus of raw growth.
Specific embodiment
The specific embodiment of the present invention is described in detail below, it is to be understood that protection scope of the present invention
It is not limited by the specific implementation.
Embodiment 1
The object of the present invention is to provide a kind of production methods of rhizopus slide sample, place and train in the first culture dish bottom
Base is supported, rhizopus is seeded in the first culture dish bottom center, is then cultivated under conditions of 25 DEG C ± 1 DEG C, forms cotton-shaped bacterium
Fall, then place pearl cotton around the first culture dish, the sprawling of the rhizoid and sporangium of rhizopus and grow on pearl cotton,
Then it is selected from pearl cotton and is distributed sparse rhizopus rhizoid and sporangium production slide sample.
Wherein, the culture medium is PDA culture medium.
Specifically when placing pearl cotton around the first culture dish, the first culture dish is placed on pearl cotton, and is guaranteed
First culture dish bottom periphery retains pearl cotton, and the first culture dish being placed on pearl cotton is integrally placed to clean filter paper
On.
The production method of above-mentioned rhizopus slide sample is selected from pearl cotton and is distributed sparse rhizopus rhizoid and spore
The method of capsule production slide sample are as follows: it is selected from pearl cotton and is distributed that sparse rhizopus is careful to be placed on glass slide, 1 drop of drop
Rhizopus on the alcohol dampening glass slide of volumetric concentration 80%, it is 3% hydrogen that 1 drop mass concentration is added when alcohol does not volatilize completely
Potassium oxide solution corrects the contraction of alcohol, material is made to restore to the original state, and it is molten then to suck extra potassium hydroxide with blotting paper
Liquid adds 1 drop of liquid sealing agent, covers cover plate, sucks extra sealing agent, i.e. production obtains complete rhizopus slide sample.
It is highly preferred that selecting the method for being distributed sparse rhizopus from pearl cotton are as follows: select and spread over from pearl cotton
The rhizopus of the visible single stain of periphery naked eyes.
In the present embodiment, sealing agent is that gum arabic, distilled water, glycerol, chloraldurate are mixed at normal temperature
It is equipped, sealing agent prescription: 30g Arabic gum, 200g chloral hydrate, 20mL glycerol, 50mL distilled water；Specifically match
Method processed are as follows: gum arabic is put into beaker, distilled water is added slowly and stirs, while by beaker heating water bath, then slowly
Slow addition chloral hydrate mixed liquor, it is stirring while adding uniform, it is eventually adding glycerol and mixes thoroughly, pour into arrow-necked bottle, cover glass
Plug, sets in 60 DEG C of incubators for 24 hours, and cleaned glass cotton and filtered through gauze are used in incubator, is stored in dark place, spare.
Wherein, pearl cotton selects pure white pearl cotton, and the density of the pearl cotton is 0.025g/cm3, compressive strength is
3.0kg/cm3, water absorption rate 0.015kg/cm3, recovery rate is greater than 98%.
Embodiment 2
As shown in Figure 1 for the mycelia sprawling of 1 head mold of embodiment encounter pearl cotton and raw growth schematic diagram, the first culture dish
1 diameter and height is respectively less than the second culture dish 2, specifically, in the present embodiment, the diameter of the first culture dish 1 is 8cm, high
Degree is 12cm, and it is highly 24cm that the diameter of the second culture dish 2, which is 15cm, a kind of production of rhizopus slide sample of the present embodiment
Method, 1 bottom of the first culture dish place culture medium 4, rhizopus is seeded in 1 bottom center of the first culture dish, then
First culture dish 1 is placed in 2 center of the second culture dish that bottom is covered with pearl cotton 3, is specifically placing pearl cotton in the first culture dish
When surrounding, the related pearl cotton 3 of the first culture dish 1 is placed in the second culture dish 2, and the first culture dish 1 is without ware lid, second
For culture dish 2 with ware lid, the pearl cotton that specific the present embodiment is selected is round, and the diameter of pearl cotton is equal to the interior of the second culture dish
Pearl cotton is laid in the second culture dish by diameter, and then upper part places the first culture dish, is then placed on 25 DEG C ± 1 DEG C
Under the conditions of cultivate, first in the first culture dish 1 formed the cotton-shaped bacterium colony 5 of rhizopus, due to the first culture dish 1 height be lower than second
Culture dish 2, the cotton-shaped bacterium colony 5 of rhizopus cross the lower culture dish 1 of height spread on pearl cotton 3 and raw growth, in pearl cotton 3
On grow complete rhizopus individual, 6 be sprawling encounter pearl cotton and the rhizopus grown, then chosen from pearl cotton
Choosing is distributed sparse rhizopus rhizoid and sporangium and makes slide sample.
Wherein, culture medium uses PDA culture medium.Specifically, selected from pearl cotton be distributed sparse rhizopus rhizoid and
The method of sporangium production slide sample are as follows:
It is selected from pearl cotton and is distributed that sparse rhizopus is careful to be placed on glass slide, 1 drop volume concentration 90% of drop
Rhizopus on alcohol dampening glass slide, it is 2% potassium hydroxide solution that 1 drop mass concentration is added when alcohol does not volatilize completely, is entangled
The contraction of positive alcohol, makes material restore to the original state, then sucks extra potassium hydroxide solution with blotting paper, adds 1 drop of liquid solid
Agent is sealed, cover plate is covered, sucks extra sealing agent, is i.e. production obtains complete rhizopus slide sample.
Specifically, selecting the method for being distributed sparse rhizopus from pearl cotton are as follows: selected from pearl cotton and spread over week
The rhizopus of the visible single stain of side naked eyes.
The sealing agent of the present embodiment is that gum arabic, distilled water, glycerol, chloraldurate are mixed at normal temperature to match
Standby to form, specific preparation method is identical with embodiment.
In the present embodiment, pearl cotton selects pure white pearl cotton, and the density of pearl cotton is 0.035g/cm3, compressive strength is
4.0kg/cm3, water absorption rate 0.015kg/cm3, recovery rate is greater than 98%.
Embodiment 3
The production method of the present embodiment rhizopus slide sample is identical with embodiment 2, unique the difference is that placing pearl
When cotton is around the first culture dish, pearl cotton is round, and the diameter of pearl cotton is equal to the interior diameter of the second culture dish, by pearl
The bottom of cotton offers slot or through-hole, and pearl cotton is laid in the second culture dish, then inlays the first culture dish ware bottom
In the slot or through-hole.
It repeats in order to prevent, description of the invention preferred embodiment, although preferred implementation of the invention has been described
Example, once a person skilled in the art knows basic creative concepts, then other change can be made to these embodiments
More and modify.So the present invention is not limited to above-mentioned specific embodiment, those skilled in the art are from above-mentioned design
It sets out, without creative labor, the various transformation made are within the scope of the present invention.
Claims (10)
1. a kind of production method of rhizopus slide sample, which is characterized in that culture medium is placed in the first culture dish bottom, by root
Mould is seeded in the bottom at the first culture dish ware bottom, then cultivates under conditions of 25 DEG C ± 1 DEG C, forms cotton-shaped bacterium colony, then put
Pearl cotton is set around the first culture dish, the sprawling of the rhizoid and sporangium of rhizopus and grow on pearl cotton, then from
It is selected on pearl cotton and is distributed sparse rhizopus rhizoid and sporangium production slide sample.
2. the production method of rhizopus slide sample according to claim 1, which is characterized in that place pearl cotton in first
When around culture dish, the first culture dish is placed on pearl cotton, and guarantees that the first culture dish bottom periphery retains pearl cotton.
3. the production method of rhizopus slide sample according to claim 1, which is characterized in that place pearl cotton in first
When around culture dish, the related pearl cotton of the first culture dish is placed in the second culture dish, the diameter and height of the first culture dish
Respectively less than the second culture dish, and the first culture dish is without ware lid, the second culture dish band ware lid.
4. the production method of rhizopus slide sample according to claim 3, which is characterized in that place pearl cotton in first
When around culture dish, pearl cotton is round, and the diameter of pearl cotton is equal to the interior diameter of the second culture dish, pearl cotton is laid on
In second culture dish, then upper part places the first culture dish.
5. the production method of rhizopus slide sample according to claim 3, which is characterized in that place pearl cotton in first
When around culture dish, pearl cotton is round, and the diameter of pearl cotton is equal to the interior diameter of the second culture dish, by the bottom of pearl cotton
Slot or through-hole are offered, pearl cotton is laid in the second culture dish, is then embedded in the first culture dish ware bottom described
In slot or through-hole.
6. the production method of rhizopus slide sample according to claim 1, which is characterized in that selected from pearl cotton point
The method of the sparse rhizopus rhizoid of cloth and sporangium production slide sample are as follows:
It is selected from pearl cotton and is distributed that sparse rhizopus is careful to be placed on glass slide, drip 1 drop volume concentration 80%~95%
Alcohol dampening glass slide on rhizopus, alcohol not completely volatilize when add 1 drop mass concentration be 2%~3% potassium hydroxide
Solution corrects the contraction of alcohol, material is made to restore to the original state, and then sucks extra potassium hydroxide solution with blotting paper, adds 1 drop
Liquid sealing agent, covers cover plate, sucks extra sealing agent, i.e. production obtains complete rhizopus slide sample.
7. the production method of rhizopus slide sample according to claim 6, which is characterized in that selected from pearl cotton point
The method of the sparse rhizopus of cloth are as follows: the rhizopus for spreading over the visible single stain of periphery naked eyes is selected from pearl cotton.
8. the production method of rhizopus slide sample according to claim 6, which is characterized in that the sealing agent be by Ah
Draw uncle's natural gum, distilled water, glycerol, chloraldurate to be mixed be equipped at normal temperature, wherein gum arabic, distilled water,
Glycerol, chloraldurate ratio be 3g:5mL:2mL:20g.
9. the production method of any one rhizopus slide sample according to claim 1~8, which is characterized in that the pearl
Cotton selects pure white pearl cotton, and the density of the pearl cotton is 0.025~0.035g/cm3, compressive strength is 3.0~4.0kg/
cm3, water absorption rate is 0.01~0.015kg/cm3, recovery rate is greater than 98%.
10. a kind of rhizopus slide sample is the production method using any one of the claim 1~8 rhizopus slide sample
It is made.
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| CN201910637380.2A CN110320079B (en) | 2019-07-15 | 2019-07-15 | Rhizopus slide specimen and manufacturing method thereof |
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| CN201910637380.2A CN110320079B (en) | 2019-07-15 | 2019-07-15 | Rhizopus slide specimen and manufacturing method thereof |
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