CN102187838B - Method for ungluing germ cells of takifugu obscurus - Google Patents
Method for ungluing germ cells of takifugu obscurus Download PDFInfo
- Publication number
- CN102187838B CN102187838B CN201110146591XA CN201110146591A CN102187838B CN 102187838 B CN102187838 B CN 102187838B CN 201110146591X A CN201110146591X A CN 201110146591XA CN 201110146591 A CN201110146591 A CN 201110146591A CN 102187838 B CN102187838 B CN 102187838B
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- Prior art keywords
- unsticking
- fertilized egg
- germ cells
- ungluing
- liquid
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a method for ungluing germ cells of takifugu obscures, and the method comprises the following steps: (1) placing the germ cells of takifugu obscures into a barrel-shaped container with a smooth inner wall, wherein the quantity of the placed germ cells is not more than 1/8 of the volume of the container; (2) slowly pouring an ungluing solution into the container, wherein the ratio of the ungluing solution to the germ cells is 4:1; (3) using a stirrer to clockwise stir the germ cells for 3-5 minutes, observing while stirring, stopping stirring when the ungluing solution is thickened, and removing the liquid on the upper layer; and (4) repeating the steps (2) and (3) for 2-3 times until the germ cells are completely unglued. The method provided by the invention has the advantages that the method for preparing the ungluing solution is simple and practicable, the ungluing effect is uniform and thorough, the degree of damages to the germ cells is reduced to the lowest, and the fineness and transparent degree of the walls of the germ cells are well ensured.
Description
Technical field
The present invention relates to a kind of method that makes fugu obscurus fertilized egg unsticking.
Background technology
Fugu belongs to (Fugu), is commonly called as filefish, creek fish, crust fish.Because the Fugu deterioration of the ecological environment; Cruel in addition fishing is excessively captured, and Fugu quantity sharply descends in recent years, so people have carried out the artificial breeding technology research of fugu obscurus and the yellow Fugu of chrysanthemum; Formed the perfect people's propagating technology of a cover; In the artificial propagation techniques of commonly using, the hatching of the viscous fertilized ovum of fugu obscurus mostly adopts artificial insemination, artificial mode of squeezing ovum to obtain fertilized fish roe, is to try hard to perfect problem in the numerous process of fugu obscurus people and make viscous fertilized ovum unsticking always.People generally adopt mud or talcum powder solution as the detackifier unsticking at present, but these viscidity-removing methods often exist the detackifier uniformity not enough; Unsticking is inhomogeneous, not thorough; With causing the fertilized egg egg membrane to adhere to the very thick unsticking material of one deck outward often; The breathing and the physiological metabolism that ovum wall fineness and transparency are fallen influence in the incubating oosperm process; Cause fugu obscurus fertilized egg hatching rate to reduce significantly; The hatching abnormal rate increases, and is time-consuming, effort and poor effect.Thereby demand urgently seeking a kind ofly save trouble, laborsaving, cost is low, viscidity-removing method efficiently, to solve the problem that exists in the unsticking flow process commonly used.
Summary of the invention
The objective of the invention is to solve and commonly use the above-mentioned defective that viscous fertilized ovum viscidity-removing method exists for a long time; Make fugu obscurus fertilized egg unsticking thorough; The ovum wall is bright and clean, and transparency is high, and then improves the fugu obscurus artificial propagation techniques; Improve the incubation rate of fugu obscurus, reduce the seed abnormal rate of making because of unsticking.
The present invention adopts following technical scheme for realizing above-mentioned purpose:
A kind of method of fugu obscurus fertilized egg unsticking may further comprise the steps:
(1) fugu obscurus fertilized egg is placed the bulk containers of inner wall smooth, fertilized egg placement amount is no more than 1/8 of vessel volume;
(2) unsticking liquid is slowly poured in the container, unsticking liquid and fertilized egg ratio are 4:1;
(3) stirred clockwise fertilized egg 3-5 minute with agitator, observe while stirring, find that unsticking liquid stops when being thick stirring, keep away upper solution;
(4) repeating step (2) and step (3) 2-3 time to fertilized egg unsticking is accomplished.
Said unsticking liquid is that sodium chloride solution and 1% the concentration of 5-6 ‰ is that 5% Klorvess Liquid is formed by 99% concentration.
Said agitator is that the surface is clean and tidy, and feather is complete, the chicken feather of moderate length.
Said unsticking liquid disposes before carrying out artificial insemination 2-3 hour, and serviceability temperature is 19 ± 1 ℃.
Unsticking liquid making method of the present invention is simple, unsticking effect uniform and complete, and the degree of simultaneously fertilized egg being damaged is reduced to minimum, has well guaranteed the fineness and the transparency of fertilized egg ovum wall after the unsticking.
Embodiment
A kind of method of fugu obscurus fertilized egg unsticking may further comprise the steps:
1. the preparation of unsticking liquid: buy medicinal physiological saline, to 5-6 ‰, in dilution, add the Klorvess Liquid (analyzing pure) of concentration 5% with distilled water diluting, addition is 1% of a dilution.
2. agitator: the chicken feather of moderate length, the chicken feather surface is clean and tidy, and feather is complete.
3. viscidity-removing method:
3.1 fugu obscurus fertilized egg is placed the bulk containers of inner wall smooth, and the material of container can be pottery, metal and plastics.Fertilized egg placement amount is no more than 1/8 of vessel volume.
Slowly pour in the container 3.2 will prepare unsticking liquid, unsticking liquid and fertilized egg ratio are 4:1.
3.3 stirred clockwise fertilized egg 3-5 minute with agitator, observe while stirring, find that unsticking liquid stops when being thick stirring, keep away upper solution.
3.4 repeat 3.2 and 3.3 steps 2-3 time, complete to the fertilized egg unsticking.
Points for attention: configuration unsticking liquid before carrying out artificial insemination 2-3 hour, guarantee during use that the temperature of unsticking liquid is 19 ± 1 ℃, identical with the water temperature of parent fish rearing.
Claims (3)
1. the method for a fugu obscurus fertilized egg unsticking may further comprise the steps:
(1) fugu obscurus fertilized egg is placed the bulk containers of inner wall smooth, fertilized egg placement amount is no more than 1/8 of vessel volume;
(2) unsticking liquid is slowly poured in the container, unsticking liquid and fertilized egg ratio are 4:1;
(3) stirred clockwise fertilized egg 3-5 minute with agitator, observe while stirring, find that unsticking liquid stops when being thick stirring, keep away upper solution;
(4) repeating step (2) and step (3) 2-3 time to fertilized egg unsticking is accomplished;
Said unsticking liquid is that sodium chloride solution and 1% the concentration of 5-6 ‰ is that 5% Klorvess Liquid is formed by 99% concentration.
2. the method for a kind of fugu obscurus fertilized egg unsticking according to claim 1 is characterized in that: said agitator is clean and tidy for the surface, and feather is complete, the chicken feather of moderate length.
3. the method for a kind of fugu obscurus fertilized egg unsticking according to claim 1 is characterized in that: said unsticking liquid disposes before carrying out artificial insemination 2-3 hour, and serviceability temperature is 19 ± 1 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110146591XA CN102187838B (en) | 2011-06-02 | 2011-06-02 | Method for ungluing germ cells of takifugu obscurus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110146591XA CN102187838B (en) | 2011-06-02 | 2011-06-02 | Method for ungluing germ cells of takifugu obscurus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102187838A CN102187838A (en) | 2011-09-21 |
| CN102187838B true CN102187838B (en) | 2012-12-19 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110146591XA Expired - Fee Related CN102187838B (en) | 2011-06-02 | 2011-06-02 | Method for ungluing germ cells of takifugu obscurus |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102939919A (en) * | 2012-10-22 | 2013-02-27 | 中国水产科学研究院黑龙江水产研究所 | Artificial unstick operation method for improving fertility rate of hemibarbus labeo roes |
| CN102939927B (en) * | 2012-11-23 | 2014-12-31 | 浙江海洋学院 | Method for improving fugu obscurus growing characters |
| CN105230537B (en) * | 2015-10-14 | 2018-03-13 | 南宁学院 | A kind of Ban Guttatus artificial insemination method |
| CN113201485A (en) * | 2021-05-10 | 2021-08-03 | 浙江省淡水水产研究所 | Fish viscous fertilized egg processing method beneficial to observation of embryo development under lens |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101933477A (en) * | 2010-08-12 | 2011-01-05 | 常熟理工学院 | Artificial propagation method of Odontobutis obscura |
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2011
- 2011-06-02 CN CN201110146591XA patent/CN102187838B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101933477A (en) * | 2010-08-12 | 2011-01-05 | 常熟理工学院 | Artificial propagation method of Odontobutis obscura |
Non-Patent Citations (5)
| Title |
|---|
| 俞录贤.青海湖裸鲤受精卵快速脱粘技术.《科学养鱼》.2009,(第10期),第11页. * |
| 徐金传等.暗纹东方鲀的实用养殖技术.《渔业致富指南》.2009,(第12期),第35-40页. * |
| 无.河豚早春人工繁殖技术.《新农村商网（http://nc.mofcom.gov.cn/news/6343466.html）》.2008,第1-2页. * |
| 朱家明等.暗纹东方鲀的人工繁殖技术研究.《水利渔业》.2002,第22卷(第6期),第12-13页. * |
| 杨玉凤.鲤、鲫鱼卵脱粘方法.《内陆水产》.2003,(第1期),第28页. * |
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| CN102187838A (en) | 2011-09-21 |
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