CN102893926B - Fertilized egg debonding technique for green pufferfish - Google Patents
Fertilized egg debonding technique for green pufferfish Download PDFInfo
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- CN102893926B CN102893926B CN201110212419.XA CN201110212419A CN102893926B CN 102893926 B CN102893926 B CN 102893926B CN 201110212419 A CN201110212419 A CN 201110212419A CN 102893926 B CN102893926 B CN 102893926B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
A fertilized egg debonding technique for the green pufferfish is used to wash off excess semen after eggs of the green pufferfish are fertilized and is characterized in that before stickiness occurs, semen is evenly splashed to a debonding cylinder which is a conical round-bottomed water cylinder with smooth inside; the debonding cylinder fully contains clean water which is the same as water for spawning and temporary culture of parent fishes in temperature and salinity, an aerator is disposed in the cylinder, the cylinder is aerated to boil the water, density of eggs in the debonding cylinder is 500-1000/L, and primary scraping is performed after the fertilized eggs absorb water to swell and egg membranes harden; a strip brush of fine and soft wool is used to scrape and brush the wall and conical bottom of the debonding cylinder to scrape off the fertilized eggs attaching to the debonding cylinder; scraping is performed once an hour for two to three times, and 90% of the fertilized eggs leave the debonding cylinder and roll with filled air in water; six hours after splashing of the eggs, 60-mesh bolting silk is used to change water for once, 18 hours after flashing of the eggs, and final scraping is performed; and aerating is stopped, the debonded fertilized eggs are sucked out by siphoning and are transferred to a fertilizer egg hatching pond.
Description
Technical field
The present invention relates to aquaculture, especially a kind of Fugu fertilized egg viscidity-removing method.
background technology
Fugu (Takifugu), is commonly called as river Puffer, belongs to Tetraodontiformes, Puffer suborder, and Molidae, Fugu belongs to.Its muscle is pure white as white, and meat flavour fat is beautiful, fresh and tender good to eat, is rich in protein, nutritious, is described as first of " the Changjiang river three delicacies ".China is the traditional habit of Jiu Youshi river Puffer fish from ancient times, and especially Jiang Xiyi Dai， river Puffer fish is regarded as the treasure on dining table always.And liver and the ovary of river Puffer fish can extract fugutoxin, are used for the treatment of neuralgia, spasm, bed-wetting etc., and economic worth and medical value are very high, have the wide prospect of earning foreign exchange.At present, the principal item of China's Fugu cultivation has fugu obscurus, Fugu rubripes, Ju Yellow Fugu, Fugu Xanthopterus (Temminck et Schlegel) and Fugu Bimaculatus Puffer etc., every annual value of production reaches several hundred million units, not only has high economic worth, also for contribution has been made in the reparation of Fugu wild resource.Flourish in view of river Puffer fish culture industry, market is huge to the demand of river Puffer fry kind.Obtain a large amount of fries and just must carry out the artificial breeding technology of touching of rule, the key technology of incubating oosperm administrative skill Fugu artificial breeding.Fugu fertilized egg has the feature of heavy property, microviscosity, during incubating oosperm, easily piles up mutually and adhesion, easily causes the incubation rate of fertilized egg to decline.
At present, traditional hatching pattern of viscous fertilized ovum has two kinds: a kind of its viscosity of utilizing, fertilized egg after fertilization is splashed to cement pit pool wall or various hatching plate, allow it stick on hatching plate, hatch, but Fugu viscosity a little less than, be easy to from hatching plate come off, at the bottom of causing fertilized egg to sink to hatchery, and then affect incubation rate; Another kind of its viscosity of removing, mostly adopt slime water or talcum powder solution to fertilized egg unsticking, these unsticking technology adapt to the fertilized egg that viscosity is strong, egg membrane is thinner mostly, Fugu fertilized egg is demersal egg, if be stained with again a large amount of detackifier (silt or talcum powder), fertilized egg more easily sinks to the bottom overstocked, affects development of fertilized ova.The Fugu fertilized egg that egg membrane is thicker so the viscidity-removing method of traditional fertilized egg is not suitable for, viscosity is weak, and two kinds of traditional method complex operations.
summary of the invention
The object of this invention is to provide a kind of viscidity-removing method simple and practical, that be applicable to Fugu fertilized egg, to adapt to the large-scale production of Fugu seed.
Technical scheme of the present invention: Fugu fish-egg after fertilization, wash away unnecessary seminal fluid, it is characterized in that before not producing viscosity, evenly splash in unsticking cylinder, unsticking cylinder adopts the round water vat of conical bottom, unsticking cylinder medial surface is smooth, unsticking cylinder fills with parent population hastens parturition and supports temporarily water equality of temperature with the clean water of salt, a gas head is set in unsticking cylinder, inflation is boiling shape, the density 500-1000 grain/L of fish-egg splashes in unsticking cylinder, treat that fertilized egg water suction expands, after egg membrane hardening, carry out wiper for the first time: by casing wall and the conical bottom of soft wool alignment gesture brushing brush unsticking cylinder, action is soft, in order to avoid stave fertilized egg, the fertilized egg being bonded on unsticking cylinder is scraped as far as possible, every one hour, wiper once, was generally scraped 2-3 time more later, and 90% fertilized egg can not stick on unsticking cylinder, in water body, with inflation, rolled, splash fish-egg after 6 hours, with 60 mesh sieve thin,tough silk, change water once, splash fish-egg after 18 hours, carry out last wiper, the fertilized egg being still bonded on unsticking cylinder is all scraped, then stop the supple of gas or steam, fish-egg sinks to the bottom, and by siphon method, the sucking-off of unsticking fertilized egg, moves into incubating oosperm pond, unsticking success.
The present invention with respect to the outstanding feature of prior art is: the unsticking of the Fugu fertilized egg that applicable egg membrane is thicker, viscosity is weak, and easy and simple to handle, method is practical, be applicable to seed large-scale production, and be easy to be accepted and promote.
Embodiment
Example 1, the yellow Fugu fertilized egg of chrysanthemum unsticking: unsticking cylinder adopts the round water vat of inflatable conical bottom, unsticking cylinder capacity 200L, in unsticking cylinder, fill water temperature 21-22 ℃, the water of salinity 13.5, a gas head is set in cylinder, inflation is boiling shape, each cylinder ten thousand fish-eggs of 15-20 (750-1000 grain/L) of splashing, after the half an hour of splashing, fertilized egg water suction is expanded, egg membrane hardening, carry out wiper for the first time: by casing wall and the conical bottom of soft wool alignment gesture brushing brush unsticking cylinder, action is soft, and the fertilized egg being bonded on unsticking cylinder is scraped as far as possible; Every one hour, wiper once, was generally scraped 2 times more later, and 90% fertilized egg is rolled with inflation in water body.Splash fish-egg after 10 hours, with 60 mesh sieve thin,tough silk, change water once, quantity of exchanged water 2/3; Splash fish-egg after 24 hours, shift out unsticking cylinder, carry out last wiper, the fertilized egg on the sticky cylinder of minute quantity is all scraped, then stop the supple of gas or steam, fish-egg sinks to the bottom, and by siphon method, the fertilized egg sucking-off of unsticking, moves into incubating oosperm pond, unsticking success.
Example 2, ungluing germ cells of takifugu obscurus: unsticking cylinder adopts the round water vat of inflatable conical bottom, unsticking cylinder capacity 200L, in unsticking cylinder, fill the fresh water of water temperature 21-22 ℃, a gas head is set in cylinder, inflation is boiling shape, each cylinder ten thousand fish-eggs of 10-15 (500-750 grain/L) of splashing, after the half an hour of splashing, fertilized egg water suction is expanded, egg membrane hardening, carry out wiper for the first time: by casing wall and the conical bottom of soft wool alignment gesture brushing brush unsticking cylinder, action is soft, and the fertilized egg being bonded on cylinder is scraped as far as possible; Every one hour, wiper once, was generally scraped 3 times more later, and 90% fertilized egg is rolled with inflation in water body.Splash fish-egg after 6 hours, with 60 mesh sieve thin,tough silk, change water once, quantity of exchanged water 2/3, splashed fish-egg after 20 hours, shift out unsticking cylinder, carry out last wiper, the fertilized egg on the sticky cylinder of minute quantity is all scraped, then stop the supple of gas or steam, fish-egg sinks to the bottom, with siphon, the fertilized egg sucking-off of unsticking, move into incubating oosperm pond, unsticking success.
Claims (1)
1. a Fugu fertilized egg viscidity-removing method, Fugu fish-egg after fertilization, wash away unnecessary seminal fluid, it is characterized in that before not producing viscosity, evenly splash in unsticking cylinder, unsticking cylinder adopts the round water vat of conical bottom, unsticking cylinder medial surface is smooth, unsticking cylinder fills with parent population hastens parturition and supports temporarily water equality of temperature with the clean water of salt, a gas head is set in unsticking cylinder, inflation is boiling shape, 500-1000/L of density of fish-egg splashes in unsticking cylinder, treat that fertilized egg water suction expands, after egg membrane hardening, carry out wiper for the first time: by casing wall and the conical bottom of soft wool alignment gesture brushing brush unsticking cylinder, action is soft, in order to avoid stave fertilized egg, the fertilized egg being bonded on unsticking cylinder is scraped as far as possible, every one hour, wiper once, was generally scraped 2-3 times more later, and 90% fertilized egg can not stick on unsticking cylinder, in water body, with inflation, rolled, splash fish-egg after 6 hours, with 60 mesh sieve thin,tough silk, change water once, splash fish-egg after 18 hours, carry out last wiper, the fertilized egg being still bonded on unsticking cylinder is all scraped, then stop the supple of gas or steam, fish-egg sinks to the bottom, and by siphon method, the sucking-off of unsticking fertilized egg, moves into incubating oosperm pond.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201110212419.XA CN102893926B (en) | 2011-07-26 | 2011-07-26 | Fertilized egg debonding technique for green pufferfish |
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| CN201110212419.XA CN102893926B (en) | 2011-07-26 | 2011-07-26 | Fertilized egg debonding technique for green pufferfish |
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| CN102893926A CN102893926A (en) | 2013-01-30 |
| CN102893926B true CN102893926B (en) | 2014-01-15 |
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| CN103828749B (en) * | 2014-03-19 | 2017-09-15 | 上海市水产研究所 | A kind of method for improving Fugu parent's postpartum survival rate |
| CN106982763B (en) * | 2017-03-30 | 2020-09-01 | 中国水产科学研究院黑龙江水产研究所 | Wild fish egg separation device and use method thereof |
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Effective date of registration: 20230803 Address after: 200433 No. 265, Jiamusi Road, Shanghai, Yangpu District Patentee after: SHANGHAI FISHERIES Research Institute SHANGHAI FISHERIES TECHNICAL EXTENSION STATION Address before: 200433 No. 265, Jiamusi Road, Shanghai, Yangpu District Patentee before: SHANGHAI FISHERIES Research Institute Patentee before: SHANGHAI FISHERIES TECHNICAL EXTENSION STATION |