CN105123573B - Fugu obscurus raun and takifugu flavidus milter cross breeding method - Google Patents

Fugu obscurus raun and takifugu flavidus milter cross breeding method Download PDF

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CN105123573B
CN105123573B CN201510446427.9A CN201510446427A CN105123573B CN 105123573 B CN105123573 B CN 105123573B CN 201510446427 A CN201510446427 A CN 201510446427A CN 105123573 B CN105123573 B CN 105123573B
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water
parent
breeding
fugu obscurus
milter
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CN105123573A (en
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张年国
潘桂平
周文玉
周裕华
刘本伟
侯文杰
毕浩
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Shanghai Fisheries Research Institute
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Shanghai Fisheries Research Institute
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

Fugu obscurus raun and takifugu flavidus milter cross breeding method, its feature include Juvenile stage, parent population reinforced cultivating, artificial insemination and hatching, larval rearing;Parent population is from 3 age above healthy matures individual, by temperature control and fortification, promotes the sexal maturity of male and female parent population synchronous;Using the insemination of artificial dry method and hatching process control measures, hatch water temperature control at 20 22 DEG C, incubation of membrane just feeds bait to it after 3 days, food species are respectively egg yolk granule → wheel animalcule → artemia nauplii/Copepods, and fry just can move it to the outer pool and carry out seedling cultivation kind when reaching 8mm.The breeding method makes the spawning rate of parent population up to more than 98%, and exomixis rate is up to 90%, and incubation rate is up to 85%;Hybrid seedlings survival rate can be made more than 80%, hybrid seedlings can be achieved and cultivate scale, batch production.

Description

Fugu obscurus raun and takifugu flavidus milter cross breeding method
Technical field
It is female that interspecific hybridization breeding technical field, especially fugu obscurus are belonged to together the invention belongs to aquaculture seawater fish Fish and the breeding method of takifugu flavidus milter Hybrid.
Background technology
Fugu obscurus (Takifugu obscurus) and takifugu flavidus (Takifugu flavidus) are under the jurisdiction of Tetraodontiformes (Tetraodontif-ormes), Molidae (Tetraodontidae), Fugu category (Fugu).Fugu obscurus is near Hai Nuanwen water borne base migration fishes, the East China Sea and the Yellow Sea, middle and lower reach of Yangtze River region are distributed mainly in China, and spring parent population is traced back River breeding migration is bred into fresh water rivers tributary, and juvenile fish is fattened in fresh water;Takifugu flavidus is commonly called as ship bar, all over the sky Star, is distributed mainly on the coastal immediate offshore area of northwest Pacific, and the East China Sea and the Yellow Sea and Bohai Offshore, category temperature are distributed mainly in China Band coastal waters demersal fishes, breed in the seawater.Recently as the increasingly mature of cultural technique, fugu obscurus and the yellow east of chrysanthemum The cultivation scale of Puffer is also constantly expanding, and both self-characteristics determine that it cultivates future, the fugu obscurus speed of growth It hurry up, 1-2 just can reach listing requirement, but its economic value is low compared with takifugu flavidus, although takifugu flavidus value is higher, but Its speed of growth is slow compared with fugu obscurus, commercial specification is can be only achieved after general 3 years, therefore develop a kind of fast growth, city Field acceptance is high, cultivation value is big new varieties are just particularly important.
According to hybrid organisms characteristic, if fugu obscurus and takifugu flavidus are hybridized, reconfigure gene, It is oriented to cultivate, it is expected to obtain the hybridization for possessing parent both sides' merit or producing the unexistent new merit of parent Kind, that is, cultivate that fast growth, individual be big, meat is good, and the river Puffer cultivation new varieties with extensive market acceptance.At present It is less about the research report in terms of Fugu class hybridization, it is concentrated mainly on takifugu flavidus and Fugu rubripes, fugu ocellatus Hybridization between Puffer, and then there is not been reported about fugu obscurus raun and takifugu flavidus milter cross breeding method.
The content of the invention
Present invention solves the technical problem that being to provide a kind of weak point for overcoming fugu obscurus and takifugu flavidus, profit The characteristics of with both each, which obtain, a kind of to be had not only having for parents' feature concurrently and grows fast advantage but also adapted to cultivation The property high excellent breed variety of strong, market acceptance.
Technical scheme is made up of following steps:
1st, Juvenile stage:Pick out individual it is healthy and strong, disease-free, without disability, body surface without parasite, body character it is clear, It is in a good state of nutrition, 3 fugu obscurus more than age is as female parent;Similarity condition screens satisfactory takifugu flavidus conduct Male parent;
2nd, parent fish rearing:By parent population individual according to female-male proportion 1:1.5 are respectively put into reinforced cultivating in indoor cement pit, water Mud sump floor space is 17 ㎡, and the depth of water is that dissolved oxygen maintains more than 5mg/L, illumination 600-800Lux, 1-2 in 60-90cm, water body It changes water once, and water temperature is maintained between 18-20 DEG C, parent fish rearing density≤5 tails/m3;Clam meat, fresh fish are fed during parent culture Animal bait, every morning 8:00-9:00 and afternoon 16:00-17:00 respectively feeds once, and each feeding volume is parent population gross weight 5%, first remove residual bait and excreta with dirt sucking tube before each bait throwing in;
3rd, period management is bred:The breeding period of fugu obscurus and takifugu flavidus distinguishes fresh water in annual 3-5 months Bred with seawater;According to the gonad development rule of two kinds of fishes, simulated ecological condition is created to the parent through reinforced cultivating:Carry For fresh fresh water and nature seawater, into water body blowing aeration with ensure the dissolved oxygen in water body higher than 5mg/L, using heating Or cooling control water temperature maintains 18-20 DEG C, illumination is maintained at 600- by sunshade net shading or light supplement irradiation 800Lux, realizes that the female fish ovaries of fugu obscurus, takifugu flavidus milter spermary are synchronously reached maturity, and can ovulate and arrange essence;
4th, artificial insemination:Fugu obscurus raun belly metamorphosis situation is observed, treats that its gonapophysis front end is full, light pressure When belly has ovum outflow and light pressure takifugu flavidus milter belly to have white seminal fluid outflow, dry method insemination method pair just can be used It carries out artificial insemination, completes hybridization;
5th, hatching management:The fish-egg of artificial insemination is directly splashed hatching, cultivating, pond are carried out into ready cement pit Interior water temperature control is at 21 ± 0.5 DEG C, and water salinity is 5 ‰, and micro- inflation, dissolved oxygen maintains more than 5mg/L;Embryonated egg is through 150- The embryonic development of 170 hours, prelarva just can incubation of membrane, the newly hatched larvae after membrane need not feed bait in 3 days, mainly according to Development by metamorphosis is completed by its endogenous nutrition;Egg yolk granule is mainly fed to fry in 4-6 days, by 200 mesh sieves of egg yolk Thin,tough silk rubs broken with the hands, with 300 mesh silk cover filterings;Wheel animalcule is fed in 7-10 days, artemia nauplii or Copepods are fed from 11 days, fry is treated When growing to 8mm, it just can move it to outdoor ponds and carry out seed rearing, going out Chi Qianxu, gradually salinization is extremely by breeding spand water salinity Cultivating pool water salinity.
The beneficial effect of cross breeding method of the present invention be the spawning rate for making parent population up to more than 98%, exomixis rate reaches 90%, incubation rate is up to 85%, and hybrid seedlings survival rate is more than 80%, successfully there is provided a kind of fugu obscurus and the yellow east of chrysanthemum The cross breeding method of square Puffer, the method belong to Fugu category in fresh water breed kind female and seawater breed kind male success The breakthrough first of hybridization;During the pond rearing of hybrid seedlings, 1 age hybridization river Puffer fish individual is big, growth is fast, premunition By force, body weight be his father, maternal 1.5 times, with obvious hybrid vigour, be expected to cultivate hybridization Fugu new lines.
Embodiment
Understand in order that the technological means that the present invention is implemented is understood, with reference to instantiation, be expanded on further, this experiment Carried out in the 4-5 months in 2014 in Jiangsu Qidong.
Fugu obscurus raun is main by following several with the technical scheme that takifugu flavidus milter belongs to interspecific hybridization breeding together Individual step composition:
1st, Juvenile stage:Pick out individual it is healthy and strong, disease-free, without disability, body surface without parasite, body character it is clear, Be in a good state of nutrition, the tail of fugu obscurus 10 in 3 ages as female parent, specification is in 1500-2500g;Similarity condition screening is conformed to The tail of takifugu flavidus 15 asked is as male parent, and specification is 1000-2000g.
2nd, parent fish rearing:By parent population individual according to female-male proportion 1:1.5 are respectively implanted reinforcing training in different parent fish rearing ponds Educate, culture pond floor space is 17 ㎡, the depth of water is 60-90cm, dissolved oxygen maintains more than 5mg/L, illumination 600-800Lux, 1-2 days Change water once, cultivate water temperature and maintain between 18-20 DEG C.To feed based on the animal baits such as clam meat, fresh fish during parent culture, Every morning 8:00-9:00 and afternoon 16:00-17:00 respectively feeds once, and each feeding volume is about the 5% of parent population gross weight, every time First residual bait and excreta are removed before bait throwing in dirt sucking tube.
3rd, period management is bred:The breeding period of fugu obscurus and takifugu flavidus distinguishes fresh water in annual 3-5 months Bred with seawater;According to the gonad development rule of two kinds of fishes, simulated ecological condition is created to the parent through reinforced cultivating:Carry For fresh fresh water and nature seawater, into water body blowing aeration with ensure the dissolved oxygen in water body higher than 5mg/L, using heating Or cooling control water temperature maintains 18-20 DEG C, illumination is maintained at 600- by sunshade net shading or light supplement irradiation 800Lux, realizes that the female fish ovaries of fugu obscurus, takifugu flavidus milter spermary are synchronously reached maturity, and can ovulate and arrange essence.
4th, artificial insemination:Fugu obscurus raun belly metamorphosis situation is observed, treats that its gonapophysis front end is full, light pressure When belly has ovum outflow and light pressure takifugu flavidus milter belly to have white seminal fluid outflow, dry method insemination method pair just can be used It carries out artificial insemination, completes hybridization.
5th, hatching management:The fish-egg of artificial insemination is directly laid into ready cement hatchery and carries out hatching training Educate, pool inner water temperature control system is at 21 ± 0.5 DEG C, and water salinity is 5 ‰, and micro- inflation, dissolved oxygen maintains more than 5mg/L;Embryonated egg passes through The embryonic development of 150-170 hours, prelarva just can incubation of membrane, the newly hatched larvae after membrane need not feed bait in 3 days, main Development by metamorphosis is completed by its endogenous nutrition;Egg yolk granule is mainly fed to fry in 4-6 days;Wheel is fed in 7-10 days Worm, feeds artemia nauplii or Copepods from 11 days, when fry grows to 8mm, just can move it to outdoor ponds and carry out seedling Kind cultivate, go out Chi Qianxu by breeding spand water salinity gradually salinization to cultivating pool water salinity.

Claims (1)

1. fugu obscurus raun and takifugu flavidus milter cross breeding method, it is characterised in that be made up of the following steps:
1), Juvenile stage:Pick out individual it is healthy and strong, disease-free, without disability, body surface without parasite, body character be clear, nutrition In order, 3 fugu obscurus more than age is as female parent, and specification is in 1500-2500g;Similarity condition screening is satisfactory Takifugu flavidus is as male parent, and specification is 1000-2000g;
2), parent fish rearing:By parent population individual according to female-male proportion 1:1.5 are respectively put into reinforced cultivating in indoor cement pit, cement Bottom of pond area is 17 ㎡, and the depth of water is 60-90cm, and dissolved oxygen maintains more than 5mg/L, illumination 600-800Lux, changes within 1-2 days water one Secondary, water temperature maintains 18-20 DEG C, parent fish rearing density≤5 tails/m3;Clam meat, fresh fish animality bait are fed during parent culture Material, every morning 8:00-9:00 and afternoon 16:00-17:00 respectively feeds once, and each feeding volume is the 5% of parent population gross weight, often First residual bait and excreta are removed before secondary bait throwing in dirt sucking tube;
3) period management, is bred:The breeding period of fugu obscurus and takifugu flavidus in annual 3-5 months, respectively in fresh water Bred with seawater;According to the gonad development rule of two kinds of fishes, simulated ecological condition is created to the parent through reinforced cultivating:Carry For fresh fresh water and nature seawater, into water body blowing aeration with ensure the dissolved oxygen in water body higher than 5mg/L, using heating Or cooling control water temperature maintains 18-20 DEG C, illumination is maintained at 600- by sunshade net shading or light supplement irradiation 800Lux, realizes that the female fish ovaries of fugu obscurus, takifugu flavidus milter spermary are synchronously reached maturity, and can ovulate and arrange essence;
4), artificial insemination:Fugu obscurus raun belly metamorphosis situation is observed, treats that its gonapophysis front end is full, gently presses abdomen , just can be using dry method insemination method to it when portion has ovum outflow and light pressure takifugu flavidus milter belly to have white seminal fluid outflow Artificial insemination is carried out, hybridization is completed;
5), hatching management:The fish-egg of artificial insemination is directly laid and carries out hatching, cultivating, pond into ready cement hatchery Interior water temperature control is at 21 ± 0.5 DEG C, and water salinity is 5 ‰, and micro- inflation, dissolved oxygen maintains more than 5mg/L;Embryonated egg is through 150- The embryonic development of 170 hours, prelarva just can incubation of membrane, the newly hatched larvae after membrane need not feed bait in 3 days, mainly according to Development by metamorphosis is completed by its endogenous nutrition;Egg yolk granule is mainly fed to fry in 4-6 days;Wheel animalcule, 11 are fed in 7-10 days It rises and feeds artemia nauplii or Copepods, when fry grows to 8mm, just can move it to outdoor ponds and carry out seed training Educate, go out Chi Qianxu by breeding spand water salinity gradually salinization to cultivating pool water salinity.
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KR101632842B1 (en) * 2015-11-18 2016-06-23 충청남도 Hybridized species of olive takifugu obscurus and takifugu rubripes, and process for preparing the same
CN106172136B (en) * 2016-07-26 2019-10-11 江苏中洋集团股份有限公司 A kind of fugu obscurus winter artificial fecundation method
CN106259118B (en) * 2016-08-24 2019-10-15 江苏省农业科学院宿迁农科所 A kind of big squama Barb fry rearing method
CN107624682A (en) * 2017-08-22 2018-01-26 大连富泰水产养殖有限公司 A kind of Fugu rubripes cultural method
CN109601433A (en) * 2019-01-08 2019-04-12 江苏中洋集团股份有限公司 The method for improving fugu obscurus raun and Fugu rubripes milter exomixis rate
CN109601438A (en) * 2019-01-28 2019-04-12 南通龙洋水产有限公司 A kind of Fugu rubripes raun and fugu obscurus milter cross breeding method
CN109819914B (en) * 2019-03-28 2021-05-25 海南晨海水产有限公司 Artificial cross breeding method for egg-shaped pompano and pompano
CN110122381A (en) * 2019-06-24 2019-08-16 北京市水产科学研究所(国家淡水渔业工程技术研究中心) A kind of mating system of ray fish

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CN1116896A (en) * 1995-03-20 1996-02-21 青岛海洋大学 Artificial fry culturing method for red-fin dongfang globe fish
JPH1023865A (en) * 1996-07-09 1998-01-27 Nisshin Flour Milling Co Ltd Raising of balloonfishes
CN102187839B (en) * 2011-06-02 2012-08-08 中国水产科学研究院淡水渔业研究中心 Artificial crossing cultivation method of seawater takifugu species

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