CN103734058B - A kind of method improving Incubation Rate of Argopecten irradians - Google Patents
A kind of method improving Incubation Rate of Argopecten irradians Download PDFInfo
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- CN103734058B CN103734058B CN201410007465.XA CN201410007465A CN103734058B CN 103734058 B CN103734058 B CN 103734058B CN 201410007465 A CN201410007465 A CN 201410007465A CN 103734058 B CN103734058 B CN 103734058B
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- hatchery
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- bay scallop
- incubation rate
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention mainly relates to marine product production technical field, especially relate to a kind of method improving Incubation Rate of Argopecten irradians.Of the present invention wash ovum method, according to fluid mechanics principle, use high intensity, the method of comprehensive inflation, sperm unnecessary in water is made the most quickly to form foam, then foam is assembled, pull out with bolting silk net, this method can effectively improve the hatching water quality of bay scallop, shorten bay scallop and wash the ovum time, incubating oosperm density is brought up to 80 100/ml, incubation rate reaches more than 65%, hatching density and incubation rate all increase significantly compared to existing technology, and improve economic benefit, have wide practical use in terms of bay scallop industrial artificial seedling rearing.
Description
Technical field
The invention mainly relates to marine product production technical field, especially relate to a kind of method improving Incubation Rate of Argopecten irradians.
Background technology
Bay scallop (Argopectenirradias Lamarck) original producton location is that U.S., Atlantic is littoral, draws from the eighties in last century
Enter China, the most become one of main breed variety of north shellfish.Bay scallop belongs to hermaphroditism, parent's shellfish spawning and fertilization
Time, sperm quantity is wayward, easily forms polyspermy, and the most too much sperm ruins water quality, makes deformity individuality increase,
Therefore Incubation Rate of Argopecten irradians is significantly lower than other bisexualism scallops aborning.The most effectively improve Incubation Rate of Argopecten irradians,
It it is one of the key issue that must solve of bay scallop industrial artificial seedling rearing.
Summary of the invention
Of the present invention wash ovum method, according to fluid mechanics principle, use high intensity, the method for comprehensive inflation, make in water
Unnecessary sperm the most quickly forms foam, is then assembled by foam, pulls out with bolting silk net, and this method can be effective
The hatching water quality improving bay scallop, shorten bay scallop and wash the ovum time, incubating oosperm density is brought up to 80-100/ml,
Incubation rate reaches more than 65%, and incubation rate increases significantly compared to existing technology, and improves economic benefit, at bay scallop
Hatching, cultivation aspect have wide practical use.
Technical scheme relates to a kind of method improving Incubation Rate of Argopecten irradians, and it includes following operating procedure,
The most 1.～3. control the triploidy rate of bay scallop in hatchery and reach 80-100/ml, repeatable operation, directly
Formed to inflation non-foam, till the clarification of hatchery water quality；
1. it is that 1.5KW aerator air port connects with the breather that internal diameter is 4cm by power, the breather mouth of pipe is inserted into hatchery
Bottom, utilize aeration fully to stir at the bottom of pond, hatchery, formed foam；
2. utilize foam collecting board at hatchery water surface paddling, 1. step is formed foam and collects to side, hatchery；
3. the foam utilizing bolting silk net step 2. to be collected drags for outside pond, hatchery.
For in technique scheme, preferably in the case of, described bay scallop germ cell, through the following steps that hatching:
30m3Hatchery, bay scallop kind shellfish 3500-3600/ piece, under 18-19 DEG C of water temperature, support temporarily accelerating bay scallop kind shellfish gonad and refer to
Number reach 15-17%, effective accumulated temperature reaches more than 400 DEG C, the avette rule of microscopy, yolk are uniform, motility of sperm good after
Hasten parturition；After drying in the shade 30-40 minute, adding the sand filtration sea water of 21-22 DEG C, kind shellfish starts to arrange essence and lays eggs.
For in technique scheme, preferably in the case of, the structure of described foam collecting board is: on the limit, long limit of stripe board
Fixing long rod handle on edge, the length of described stripe board is slightly less than the side of rectangle hatchery.The length of described long rod handle connects
The height of nearly user, facilitates user gripping long rod handle to complete that 1. step is formed foam and collects the purpose to side, hatchery.
Beneficial effect:
Tradition gas stone aeration, several places fixed point can only inflate in hatchery, and inflation dispersion, narrow-minded, formation of foam is slow and lacks.This
In invention, aerator only connects an escape pipe, and gas concentrates ejection from the mouth of pipe, large-minded and violent, according to fluid mechanics principle, acute
Strong water layer fluctuation is more beneficial for unnecessary seminal fluid in water and quickly arrives water surface with gas, forms foam, side the most of the present invention
Method, can carry out aeration in water body any point, it is ensured that the uniformity of inflation and popularity.The method of the invention is by acutely
Aeration makes the formation foam of sperm rapid, high volume in water, then foam concentrations is pulled out, so repeats, can be in relatively short period of time
Make spermiogenesis tail foam in water thoroughly discharge, complete to wash ovum, no matter from wash ovum effect or ageing in terms of will be far better than biography
Ovum is washed in the gas stone inflation of system.Using this method to wash ovum 60-80 minute, in water, unnecessary sperm just can the most thoroughly be discharged, in hatching
Under conditions of water temperature 21-22 DEG C, the hatching density of germ cell can reach 80-100/ml, and incubation rate reaches more than 65%, with
Tradition is washed ovum method and is compared to shorten and wash the ovum time, while improve hatching density and unit of water body utilization rate, also improves
Incubation rate, had both reduced production cost, had added again economic benefit.Additionally, the production technology of this product is simple, it is easy to expand rule
Mould produces, easy control of process conditions.
Detailed description of the invention
Following non-limiting example can make those of ordinary skill in the art that the present invention is more fully understood, but not with any side
Formula limits the present invention.
Embodiment 1
Under 18-19 DEG C of water temperature condition, supporting accelerating bay scallop kind shellfish genital gland indices temporarily and reach 15-17%, effective accumulated temperature reaches 400 DEG C
Above, the avette rule of microscopy, yolk is uniform, and motility of sperm the most just can carry out artificial induced spawning.Dry in the shade 30-40 minute,
Being subsequently adding the sand filtration sea water of 21-22 DEG C, latter about the 40 minutes kind shellfishes that add water start to arrange essence and lay eggs.30m3Hatchery, plant shellfish
3500-3600/ piece, after finding kind of shellfish row's essence, 15-18 minute triploidy rate can reach 80-100/ml, then will all plant shellfish
Move into another hatchery having filled it up with 21-22 DEG C of sea water and continue lay eggs (generally going through the shifting pond of 4-5 time, bay scallop laying eggs terminates).
Once plant shellfish to remove and soon this hatchery is carried out aeration and wash ovum, i.e. according to following steps 1-3 repeatable operation, until inflation nothing
Formation of foam, till the clarification of hatchery water quality.
1. inflation, forms foam
Aerator air port is connected with the mouth of pipe of breather one end, and the mouth of pipe of the breather other end is fixed on holding rod, holding rod
Length according to the length and width of hatchery and the degree of depth depending on, operator utilizes this holding rod, the breather mouth of pipe can be inserted into hatchery
Any position (predominantly to center and the bottom ventilation of hatchery).
During hatching, starting aerator, operator can hold holding rod, is placed in by the mouth of pipe bottom hatchery, around pond 1-2 week, profit
Fully stir hatchery with aeration, the sperm in water arrives surface, hatchery along with gas, forms foam.
During hatching, with 30m3As a example by hatchery, the external flexible metal conduit meeting an internal diameter 4cm of power 1.5KW aerator, profit
With holding rod, can the breather mouth of pipe be inserted into bottom hatchery, around Chi1-2Quan, at uniform aeration extensive at the bottom of pond, unnecessary in water
Sperm arrives surface, hatchery with gas, forms a large amount of foam.
2. collect foam
Utilizing foam collecting board to collect the foam that step 1 is formed, wherein, the structure of described foam collecting board is: in stripe board
Long side edge on fix long rod handle, the length of described stripe board is slightly less than the side of rectangle hatchery.Described long rod handle
Length close to the height of user, it is simple to operator stands hand-held in both sides, hatchery, and two operators' hand-held foams respectively are received
Two handles of collection plate, walk from one end, hatchery to the other end, and step 1, at hatchery water surface paddling, is formed by foam collecting board
Foam is collected to less region, side, hatchery, in order to pull out.
3. pull foam out
Utilizing iron wire to make racket shape framework, then gravity flow screen tulle on framework, operator may utilize this instrument in hatchery
The water surface streaks, and just can be dragged for outside pond by the foam that step 2 is assembled, and treats that foam arrives bolting silk net less and cannot pull out, just can stop this step
Operation.
With 30m3As a example by the hatchery of volume, under conditions of hatching water temperature 21-22 DEG C, the hatching density 80-100 of germ cell
/ ml, uses this method to wash ovum 60-80 minute, and incubation rate reaches can be to more than 65%, and this method, according to fluid mechanics principle, is passed through
Increase inflation intensity and inflation point position, make unnecessary sperm in water quickly form foam, collect in conjunction with foam, pull operation sequence out
Carry out washing ovum, wash ovum method with tradition compared with not only substantially reduce and wash the ovum time, and improve hatching density and incubation rate.
Embodiment 2
2008-2012 years, within continuous 5 years, the breeding center of drawing Research Institute of Ocean Fishery Science, Liaoning Province subordinate carried out bay
Scallop artificial breeding produces.Utilize the method described in embodiment 1, bay scallop kind shellfish lay eggs row essence 15-18 minute after, will kind
Shellfish is lifted to other hatcheries, is then added up the germ cell quantity of hatchery by multipoint random sampling quantitative analysis method,
Ensure that hatchery triploidy rate is maintained at 80-100/ml.Carry out immediately washing ovum operation, recycle step 1-3 in this method,
Through the ovum of washing of 60-80 minute, hatchery non-foam was formed, and water colour is become by muddiness more to be clarified, and washes ovum and completes.Wait to be fertilized
The quantity of D type germling, to D type germling, the method still using multiple spot to sample quantitative analysis immediately, is added up by egg development.
Incubation rate (%)=(D type germling number/germ cell number) × 100, statistical result over the years is shown in Table 1.
According to the production practices of continuous 5 years, 30m3Hatchery, incubating oosperm density 80-100/ml, use 1.5KW
Aerator, join internal diameter 4cm flexible metal conduit inflation, wash 60-80 minute ovum time, final incubation rate can reach more than 65%.
Statistical result data understand: produce water body 1800m3, wherein seedling water 1000m3, water body 800m cultivated by bait3, every year
Average output commercial seedling 2-3 hundred million.
1.2008 years-2012 years Artificial Rearing of Scallop production leadtime results of table
The above, the only present invention preferably detailed description of the invention, but protection scope of the present invention is not limited only to this, and any ripe
Know those skilled in the art in the technical scope that the invention discloses, according to the technical program and inventive concept thereof in addition etc.
With replacing or changing, all should contain within protection scope of the present invention.
Claims (1)
1. the method improving Incubation Rate of Argopecten irradians, it is characterised in that include following operating procedure,
The most 1.～3. control the triploidy rate of bay scallop in hatchery and reach 80-100/ml, repeatable operation, directly
Formed to inflation non-foam；
1. it is that 1.5KW aerator air port connects with the breather that internal diameter is 4cm by power, the mouth of pipe of described breather is inserted into
The bottom of hatchery, at the bottom of abundant aeration agitation pond, hatchery, forms foam；
2. utilize foam collecting board at hatchery water surface paddling, 1. step is formed foam and collects to side, hatchery；
3. the foam utilizing bolting silk net step 2. to be collected drags for outside pond, hatchery；
The germ cell of described bay scallop, through the following steps that hatching: 30m3Hatchery, bay scallop kind shellfish 3500-3600
Piece, under 18-19 DEG C of water temperature, to support accelerating bay scallop kind shellfish genital gland indices temporarily and reach 15-17%, effective accumulated temperature reaches more than 400 DEG C,
The avette rule of microscopy, yolk are uniform, motility of sperm good after hasten parturition；After drying in the shade 30-40 minute, add 21-22 DEG C
Sand filtration sea water, plant shellfish and start to arrange essence and lay eggs；
The structure of described foam collecting board is: fix long rod handle, the length of described stripe board in the long side edge of stripe board
It is slightly less than the side of rectangle hatchery.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410007465.XA CN103734058B (en) | 2013-08-01 | 2014-01-07 | A kind of method improving Incubation Rate of Argopecten irradians |
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310332358XA CN103371118A (en) | 2013-08-01 | 2013-08-01 | Method for improving bay scallop hatchability |
| CN201310332358X | 2013-08-01 | ||
| CN201310332358.X | 2013-08-01 | ||
| CN201410007465.XA CN103734058B (en) | 2013-08-01 | 2014-01-07 | A kind of method improving Incubation Rate of Argopecten irradians |
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| Publication Number | Publication Date |
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| CN103734058A CN103734058A (en) | 2014-04-23 |
| CN103734058B true CN103734058B (en) | 2016-07-13 |
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| CN201310332358XA Pending CN103371118A (en) | 2013-08-01 | 2013-08-01 | Method for improving bay scallop hatchability |
| CN201410007465.XA Expired - Fee Related CN103734058B (en) | 2013-08-01 | 2014-01-07 | A kind of method improving Incubation Rate of Argopecten irradians |
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| CN201310332358XA Pending CN103371118A (en) | 2013-08-01 | 2013-08-01 | Method for improving bay scallop hatchability |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103583439A (en) * | 2013-11-20 | 2014-02-19 | 文登市水产技术推广站 | Method for improving egg washing efficiency of bay scallops |
| CN104585122A (en) * | 2014-12-30 | 2015-05-06 | 浙江海洋学院 | Foam removing device |
| CN104585113A (en) * | 2014-12-30 | 2015-05-06 | 浙江海洋学院 | Super-intensive breeding device with low circulation rate |
| CN107232103B (en) * | 2017-07-06 | 2020-03-20 | 中国海洋大学 | Method for determining temperature of bivalve shellfish for accumulated temperature and ripening |
| CN109287533B (en) * | 2018-09-17 | 2024-02-27 | 大连海洋大学 | Large-scale breeding method and equipment for hybrid scallop offspring seeds |
| CN116391654B (en) * | 2023-03-30 | 2023-11-03 | 中国海洋大学 | Large-scale hybridization breeding method for hermaphrodite scallops |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1039168A (en) * | 1988-07-13 | 1990-01-31 | 杜佳垠 | A kind of method for culturing young scallop in gulf |
| US5144907A (en) * | 1988-10-06 | 1992-09-08 | Dabinett Patrick E | Scallop aquaculture |
| CN101099453A (en) * | 2007-07-14 | 2008-01-09 | 中国海洋大学 | Bay scallop laying eggs and hatching method |
| CN101703017A (en) * | 2009-07-31 | 2010-05-12 | 青岛农业大学 | Technique for preparing hermaphrodite scallop interspecific hybrid fingerlings |
| CN102217561A (en) * | 2010-04-13 | 2011-10-19 | 中国科学院海洋研究所 | Method for preparing male sterile bodies of argopecten irradians |
| CN102308770A (en) * | 2011-08-05 | 2012-01-11 | 青岛农业大学 | Breeding method for new marine backcrossed scallop variety |
-
2013
- 2013-08-01 CN CN201310332358XA patent/CN103371118A/en active Pending
-
2014
- 2014-01-07 CN CN201410007465.XA patent/CN103734058B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1039168A (en) * | 1988-07-13 | 1990-01-31 | 杜佳垠 | A kind of method for culturing young scallop in gulf |
| US5144907A (en) * | 1988-10-06 | 1992-09-08 | Dabinett Patrick E | Scallop aquaculture |
| CN101099453A (en) * | 2007-07-14 | 2008-01-09 | 中国海洋大学 | Bay scallop laying eggs and hatching method |
| CN101703017A (en) * | 2009-07-31 | 2010-05-12 | 青岛农业大学 | Technique for preparing hermaphrodite scallop interspecific hybrid fingerlings |
| CN102217561A (en) * | 2010-04-13 | 2011-10-19 | 中国科学院海洋研究所 | Method for preparing male sterile bodies of argopecten irradians |
| CN102308770A (en) * | 2011-08-05 | 2012-01-11 | 青岛农业大学 | Breeding method for new marine backcrossed scallop variety |
Non-Patent Citations (2)
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| 提高海湾扇贝室内人工育苗孵化率的技术要点;王海涛等;《齐鲁渔业》;20101215;第27卷(第12期);第25页第13行-33行、第26页左栏倒数第8行-右栏最后1行 * |
| 浅谈提高海湾扇贝人工育苗孵化率的技术措施;顾成柏等;《科学养鱼》;20081210(第12期);第22-23页 * |
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| Publication number | Publication date |
|---|---|
| CN103371118A (en) | 2013-10-30 |
| CN103734058A (en) | 2014-04-23 |
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