CN105230537B - A kind of Ban Guttatus artificial insemination method - Google Patents
A kind of Ban Guttatus artificial insemination method Download PDFInfo
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- CN105230537B CN105230537B CN201510660298.3A CN201510660298A CN105230537B CN 105230537 B CN105230537 B CN 105230537B CN 201510660298 A CN201510660298 A CN 201510660298A CN 105230537 B CN105230537 B CN 105230537B
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- 238000000034 method Methods 0.000 title claims abstract description 24
- 230000009027 insemination Effects 0.000 title claims abstract description 22
- 210000000582 semen Anatomy 0.000 claims abstract description 35
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 24
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 24
- 210000004681 ovum Anatomy 0.000 claims abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 17
- 239000000919 ceramic Substances 0.000 claims abstract description 16
- 239000002504 physiological saline solution Substances 0.000 claims abstract description 16
- 238000001914 filtration Methods 0.000 claims abstract description 13
- 238000002156 mixing Methods 0.000 claims abstract description 13
- 239000004677 Nylon Substances 0.000 claims abstract description 9
- 229920001778 nylon Polymers 0.000 claims abstract description 9
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 17
- 238000003756 stirring Methods 0.000 claims description 13
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 8
- 229910001570 bauxite Inorganic materials 0.000 claims description 8
- 239000000843 powder Substances 0.000 claims description 8
- 239000011780 sodium chloride Substances 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 3
- 230000004720 fertilization Effects 0.000 abstract description 9
- 230000012447 hatching Effects 0.000 abstract description 7
- 241000251539 Vertebrata <Metazoa> Species 0.000 abstract description 2
- 230000013020 embryo development Effects 0.000 description 8
- 239000010984 cultured pearl Substances 0.000 description 7
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 210000001161 mammalian embryo Anatomy 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 210000002808 connective tissue Anatomy 0.000 description 2
- 238000001125 extrusion Methods 0.000 description 2
- 230000032696 parturition Effects 0.000 description 2
- 206010003497 Asphyxia Diseases 0.000 description 1
- 241000555268 Dendroides Species 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000026109 gonad development Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The present invention relates to vertebrate cultural technique, and in particular to a kind of spot Guttatus artificial insemination method, takes out the spermary of spot Guttatus milters first, is shredded, it is in rotten shape to be ground to spermary, and it is extruded with 100 150 mesh nylon bolting silk nets, obtains seminal fluid；After seminal fluid is well mixed with the physiological saline that concentration is 0.67 0.70%, ceramic bowl is transferred to, in the case where temperature is 15 20 DEG C, after mixing 4 8min with fish-egg, obtains embryonated egg；Add water into ceramic bowl, stand 28 35min, filtering, acquisition ovum footpath >=3mm embryonated egg；After ovum footpath >=3mm embryonated egg and detackifier are stirred into 5 10min, filtering, wash 35 times, obtain embryonated egg to be hatched, rate of fertilization of the invention is high, and Fertilized quality is good, is advantageous to save hatching cost and improves hatching efficiency.
Description
Technical field
The present invention relates to vertebrate cultural technique, and in particular to a kind of Ban Guttatus artificial insemination method.
Background technology
Spot Of-digestive-tract is a species of Chang sections Of-digestive-tract category, enjoys the good reputation of " king of fresh water " and the Zhujiang River " four big fishes ", its meat
Delicacy, delicious, nutritive value is high, deep to be liked thering is wide promotion prospect, but its quantity by consumers in general and culturist
It is rare, it is impossible to meet market needs, therefore, technology of artificial insemination Wei Ban Guttatus batch production provide a new approach.
In the prior art, such as document《Ban Guttatus growth, breeding, feeding habits and cultural technique》(Li Hongjing, Chu Zhangjie, lake
Northern agricultural sciences, 2006,45 (6):807-809)、《Ban Guttatus artificial induced spawning technical essentials》(Li Junmin, Li Yan, science are breeded fish,
1998(6):21-22)、《The Primary Study of Ban Guttatus artificial propagations》(circuit, Zhou Tao, Yang Kai etc., Guangdong Ocean University's journal,
2008,28 (4):83-84)、《Pond cultured pearls Jiang Ban Guttatus artificial propagations and the Primary Study of embryonic development》(Zhu Xin is put down, and old Kun is kind, thanks
Just etc., Dalian aquatic product Academy journal, 2005,20 (4):352-354), equal Dui Ban Guttatus have carried out the research of profound level, Dan Ban Guttatus people
The development of work insemination techniques is shorter, still suffers from following problems：1. spot Guttatus milters spermary is in dendroid, it is difficult to extrudes seminal fluid, Jin Erzeng
Add the cost of artificial insemination；2. when extracting seminal fluid, the connective tissue in seminal fluid is easily adhered on fish-egg, meanwhile, fish-egg belongs to
Viscid egg, therefore in sperm ovum binding, adhesion is excessive, easily causes embryonated egg death by suffocation, reduces the incubation rate of embryonated egg, enters
And influence Ban Guttatus yield.
The content of the invention
It is an object of the invention to provide a kind of Ban Guttatus artificial insemination method, for solving the combination of Ban Guttatus parent populations embryonated egg
Insufficient and viscous fertilized ovum meets water and is frozen into pieces of an egg, causes rate of fertilization, incubation rate low, and then influence asking for its breeding production
Topic.
It is achieved especially by following scheme：
A kind of Ban Guttatus artificial insemination method, it is characterised in that comprise the following steps：
(1) Ban Guttatus milters are dissected, spermary is taken out, is shredded, it is in rotten shape to be ground to spermary, and it is used into 100-150 mesh Buddhist nuns
Imperial bolting silk net extrusion, obtains seminal fluid；
(2) after seminal fluid is well mixed with the physiological saline that concentration is 0.67-0.70%, ceramic bowl is transferred to, is 15- in temperature
At 20 DEG C, after mixing 4-8min with fish-egg, embryonated egg is obtained；
(3) add water into ceramic bowl, stand 28-35min, filtering, acquisition ovum footpath >=3mm embryonated egg；
(4) it is in 18-23 DEG C in temperature, after ovum footpath >=3mm embryonated egg and detackifier stirring 5-10min, filters, water
Wash 3-5 times, obtain embryonated egg to be hatched.
The nylon bolting silk net is 120 mesh.
The volume ratio of the seminal fluid and physiological saline is 1:10-15.
The volume ratio of the seminal fluid and fish-egg is 1:100-120.
The volume ratio of the embryonated egg and detackifier is 1-1.5：10.
It is 3-3.5 by weight by diatomite, bauxite powder, sodium chloride, water that the detackifier, which is,:0.75-1：1：40
Mix.
The stirring, its rotating speed are 30-50r/min.
Beneficial effects of the present invention
The present invention is used and first dissected, then grinds spermary to rotten shape, is advantageous to the extrusion of seminal fluid, and ensure the normal of sperm
Rate, spermary material then is extruded using bolting silk net, while fully extraction milter seminal fluid, the connective tissue for avoiding spermary glues
It is attached on egg cell, is advantageous to smart ovum and fully combines, and combine the use of physiological saline, promotes gonad development, form larger
Spermary wall, and then improve rate of fertilization；Simultaneously by Process of Insemination temperature control at 15-20 DEG C, ensure that smart ovum is survived and is in work
Jump state, be advantageous to smart ovum and quickly combine；In conjunction with the application of detackifier, by diatomite, bauxite powder, sodium chloride by rationally matching somebody with somebody
Than detackifier is made so that viscous fertilized ovum equably unsticking, then coordinate the control of the concentration of detackifier, temperature in use, will be by
Smart ovum injury tolerance minimizes, and then improves the incubation rate of embryonated egg, meanwhile, the impurity on embryonated egg surface is removed, and then
The finish and transparency of embryonated egg ovum wall after unsticking are ensure that, and then is easy to observe embryo's situation.By using according to《Support in pond
Zhujiang River Ban Guttatus artificial propagations and the Primary Study of embryonic development》After scheme is hastened parturition to female Ban Guttatus, using the present invention program
Progress artificial insemination, and according to《Pond cultured pearls Jiang Ban Guttatus artificial propagations and the Primary Study of embryonic development》Method is entered to embryonated egg
Row hatching, as a result shows, the rate of fertilization of the present invention program is higher, is approximately higher than《Pond cultured pearls Jiang Ban Guttatus artificial propagations and embryonic development
Primary Study》1.2 times of rate of fertilization, hatch time ratio《Pond cultured pearls Jiang Ban Guttatus artificial propagations and the Primary Study of embryonic development》
Few 5h or so, explanation：The present invention program rate of fertilization is high, and Fertilized quality is good, is advantageous to save hatching cost and improves hatching effect
Rate.
Embodiment
Limited with reference to specific embodiment technical scheme is further, but claimed
Scope is not only limited to made description.
Embodiment 1
A kind of Ban Guttatus artificial insemination method, it is characterised in that comprise the following steps：
(1) Ban Guttatus milters are dissected, spermary is taken out, is shredded, it is in rotten shape to be ground to spermary, by it with 120 mesh nylon mesh
Tulle is extruded, and obtains seminal fluid；
(2) after seminal fluid is well mixed with the physiological saline that concentration is 0.68%, ceramic bowl is transferred to, in the case where temperature is 15 DEG C,
After mixing 5min with fish-egg, embryonated egg is obtained；
(3) add water into ceramic bowl, stand 30min, filtering, acquisition ovum footpath >=3mm embryonated egg；
(4) it is in 18-20 DEG C in temperature, after ovum footpath >=3mm embryonated egg and detackifier stirring 8min, filtering, washes 2
It is secondary, obtain embryonated egg to be hatched.
The volume ratio of the seminal fluid and physiological saline is 1:15.
The volume ratio of the seminal fluid and fish-egg is 1:110.
The volume ratio of the embryonated egg and detackifier is 1：12.
It is 3 by weight by diatomite, bauxite powder, sodium chloride, water that the detackifier, which is,：1：1：40 mix.
The stirring, its rotating speed are 45r/min.
Embodiment 2
A kind of Ban Guttatus artificial insemination method, it is characterised in that comprise the following steps：
(1) Ban Guttatus milters are dissected, spermary is taken out, is shredded, it is in rotten shape to be ground to spermary, by it with 100 mesh nylon mesh
Tulle is extruded, and obtains seminal fluid；
(2) after seminal fluid is well mixed with the physiological saline that concentration is 0.70%, ceramic bowl is transferred to, in the case where temperature is 18 DEG C,
After mixing 4min with fish-egg, embryonated egg is obtained；
(3) add water into ceramic bowl, stand 28min, filtering, acquisition ovum footpath >=3mm embryonated egg；
(4) it is in 21-23 DEG C in temperature, after ovum footpath >=3mm embryonated egg and detackifier stirring 5min, filtering, washes 4
It is secondary, obtain embryonated egg to be hatched.
The volume ratio of the seminal fluid and physiological saline is 1:12.
The volume ratio of the seminal fluid and fish-egg is 1:100.
The volume ratio of the embryonated egg and detackifier is 1：8.
It is 3.2 by weight by diatomite, bauxite powder, sodium chloride, water that the detackifier, which is,：0.8：1：40 mixing and
Into.
The stirring, its rotating speed are 42r/min.
Embodiment 3
A kind of Ban Guttatus artificial insemination method, it is characterised in that comprise the following steps：
(1) Ban Guttatus milters are dissected, spermary is taken out, is shredded, it is in rotten shape to be ground to spermary, by it with 150 mesh nylon mesh
Tulle is extruded, and obtains seminal fluid；
(2) after seminal fluid is well mixed with the physiological saline that concentration is 0.67%, ceramic bowl is transferred to, in the case where temperature is 20 DEG C,
After mixing 8min with fish-egg, embryonated egg is obtained；
(3) add water into ceramic bowl, stand 35min, filtering, acquisition ovum footpath >=3mm embryonated egg；
(4) it is in 19-21 DEG C in temperature, after ovum footpath >=3mm embryonated egg and detackifier stirring 10min, filters, washing
5 times, obtain embryonated egg to be hatched.
The volume ratio of the seminal fluid and physiological saline is 1:10.
The volume ratio of the seminal fluid and fish-egg is 1:110.
The volume ratio of the embryonated egg and detackifier is 1.2：10.
It is 3.5 by weight by diatomite, bauxite powder, sodium chloride, water that the detackifier, which is,：1：1：40 mixing and
Into.
The stirring, its rotating speed are 50r/min.
Embodiment 4
A kind of Ban Guttatus artificial insemination method, it is characterised in that comprise the following steps：
(1) Ban Guttatus milters are dissected, spermary is taken out, is shredded, it is in rotten shape to be ground to spermary, by it with 120 mesh nylon mesh
Tulle is extruded, and obtains seminal fluid；
(2) after seminal fluid is well mixed with the physiological saline that concentration is 0.69%, ceramic bowl is transferred to, in the case where temperature is 16 DEG C,
After mixing 4min with fish-egg, embryonated egg is obtained；
(3) add water into ceramic bowl, stand 32min, filtering, acquisition ovum footpath >=3mm embryonated egg；
(4) it is in 20-23 DEG C in temperature, after ovum footpath >=3mm embryonated egg and detackifier stirring 7min, filtering, washes 3
It is secondary, obtain embryonated egg to be hatched.
The volume ratio of the seminal fluid and physiological saline is 1:10.
The volume ratio of the seminal fluid and fish-egg is 1:120.
The volume ratio of the embryonated egg and detackifier is 1：10.
It is 3.5 by weight by diatomite, bauxite powder, sodium chloride, water that the detackifier, which is,：0.9：1：40 mixing and
Into.
The stirring, its rotating speed are 39r/min.
Embodiment 5
A kind of Ban Guttatus artificial insemination method, it is characterised in that comprise the following steps：
(1) Ban Guttatus milters are dissected, spermary is taken out, is shredded, it is in rotten shape to be ground to spermary, by it with 150 mesh nylon mesh
Tulle is extruded, and obtains seminal fluid；
(2) after seminal fluid is well mixed with the physiological saline that concentration is 0.70%, ceramic bowl is transferred to, in the case where temperature is 20 DEG C,
After mixing 6min with fish-egg, embryonated egg is obtained；
(3) add water into ceramic bowl, stand 30min, filtering, acquisition ovum footpath >=3mm embryonated egg；
(4) it is in 19-21 DEG C in temperature, after ovum footpath >=3mm embryonated egg and detackifier stirring 10min, filters, cleaning
7 times, obtain embryonated egg to be hatched.
The volume ratio of the seminal fluid and physiological saline is 1:10.
The volume ratio of the seminal fluid and fish-egg is 1:100.
The volume ratio of the embryonated egg and detackifier is 1.2：10.
It is 3.5 by weight by diatomite, bauxite powder, sodium chloride, water that the detackifier, which is,：1：1：40 mixing and
Into.
The stirring, its rotating speed are 44r/min.
Test example 1
1. according to《Pond cultured pearls Jiang Ban Guttatus artificial propagations and the Primary Study of embryonic development》Record is hastened parturition after female Ban Guttatus, is led to
Cross embodiment 1-5 schemes to be fertilized, and calculate rate of fertilization, as a result as shown in table 1：
Table 1
2. according to《Pond cultured pearls Jiang Ban Guttatus artificial propagations and the Primary Study of embryonic development》Method is obtained to embodiment 1-5
Embryonated egg to be hatched hatched, record embryo's each stage development take, as shown in table 2：
Table 2
As shown in Table 1, the rate of fertilization using embodiment 1-5 schemes is higher, in conjunction with table 2, the embryonated egg of the present embodiment
Brooding time is shorter, enters gastrul stage through 14h30min, embryo hatching required time is about 50h, is compared《Pond cultured pearls Jiang Ban Guttatus people
Work is bred and the Primary Study of embryonic development》Record, shorten the brooding time of nearly 5 hours, saved hatching cost, said
The Fertilized quality of bright the present invention program is more excellent, and development condition is preferable, and rate of fertilization is preferable.
Claims (6)
1. a kind of Ban Guttatus artificial insemination method, it is characterised in that comprise the following steps：
(1) Ban Guttatus milters are dissected, spermary is taken out, is shredded, it is in rotten shape to be ground to spermary, and it is used into 100-150 mesh nylon mesh
Tulle is extruded, and obtains seminal fluid；
(2) after seminal fluid is well mixed with the physiological saline that concentration is 0.67-0.70%, ceramic bowl is transferred to, is 15-20 DEG C in temperature
Under, after mixing 4-8min with fish-egg, obtain embryonated egg；
(3) add water into ceramic bowl, stand 28-35min, filtering, acquisition ovum footpath >=3mm embryonated egg；
(4) it is in 18-23 DEG C in temperature, after ovum footpath >=3mm embryonated egg and detackifier stirring 5-10min, filtering, washes 3-
5 times, obtain embryonated egg to be hatched；
It is 3-3.5 by weight by diatomite, bauxite powder, sodium chloride, water that the detackifier, which is,：0.75-1：1：40 mixing
Form.
2. spot Guttatus as claimed in claim 1 artificial insemination method, it is characterised in that nylon bolting silk net is 120 mesh.
3. Ban Guttatus as claimed in claim 1 artificial insemination method, it is characterised in that the volume of the seminal fluid and physiological saline
Than for 1:10.
4. Ban Guttatus as claimed in claim 1 artificial insemination method, it is characterised in that the seminal fluid, mixed liquor of normal saline
Volume ratio with fish-egg is 1:100-120.
5. Ban Guttatus as claimed in claim 1 artificial insemination method, it is characterised in that the volume of the embryonated egg and detackifier
Than for 1-1.5：10.
6. Ban Guttatus as claimed in claim 1 artificial insemination method, it is characterised in that stirred in the step (2) and step (4)
Mix, its rotating speed is 30-50r/min.
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| CN106172146B (en) * | 2016-09-21 | 2019-05-14 | 南宁学院 | A kind of artificial insemination method of rainbow trout |
| CN106879515A (en) * | 2017-04-12 | 2017-06-23 | 布尔津县额河特有冷水鱼繁育开发有限公司 | A kind of viscidity-removing method of body graining embryonated egg high |
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| CN102187838A (en) * | 2011-06-02 | 2011-09-21 | 中国水产科学研究院淡水渔业研究中心 | Method for ungluing germ cells of takifugu obscurus |
| CN102613125A (en) * | 2012-04-26 | 2012-08-01 | 云南省渔业科学研究院 | Artificial insemination method of mystus nemurus |
| CN103202251A (en) * | 2013-04-08 | 2013-07-17 | 常熟理工学院 | Odontobutisobscura temperature-control flowing-water hatching method |
| CN103563799A (en) * | 2012-08-03 | 2014-02-12 | 潘洪强 | Method for breeding allogynogenetic crucian carp fry at low temperature |
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2015
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Patent Citations (4)
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| CN102187838A (en) * | 2011-06-02 | 2011-09-21 | 中国水产科学研究院淡水渔业研究中心 | Method for ungluing germ cells of takifugu obscurus |
| CN102613125A (en) * | 2012-04-26 | 2012-08-01 | 云南省渔业科学研究院 | Artificial insemination method of mystus nemurus |
| CN103563799A (en) * | 2012-08-03 | 2014-02-12 | 潘洪强 | Method for breeding allogynogenetic crucian carp fry at low temperature |
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Effective date of registration: 20221024 Address after: 902-b, building 5, 158 Xisan Road, Tianjin Binhai New Area pilot free trade zone (Airport Economic Zone) Patentee after: Tianjin Saiyi Automation Equipment Technology Co.,Ltd. Address before: 530200 No. 8, Ting Ting Road, Yongning District, Nanning, the Guangxi Zhuang Autonomous Region Patentee before: NANNING University |