CN106489801B - A kind of half pierces thick lip fish artificial seedling rearing's method - Google Patents
A kind of half pierces thick lip fish artificial seedling rearing's method Download PDFInfo
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- 230000037396 body weight Effects 0.000 claims description 20
- 102000002322 Egg Proteins Human genes 0.000 claims description 18
- 108010000912 Egg Proteins Proteins 0.000 claims description 18
- 210000004681 ovum Anatomy 0.000 claims description 18
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- 235000013601 eggs Nutrition 0.000 claims description 13
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses a kind of half pierce thick lip fish (Acrossocheilius hemispinus) artificial seedling rearing's method, including from natural water area capture original seed parent population, parent fish rearing, antenatal reinforced cultivating, artificial induced spawning, artificial insemination, incubating oosperm, young juvenile fish cement pit artificial culture and large size seedling seed Tu Chi cultivate and form and etc..The present invention brings out first filial generation juvenile fish, using cultivation in 2 years, cultivates sexually matured first generation filial generation parent population using the wild parent population cultivated, artificial breeding；Second filial juvenile fish is brought out using the first generation filial generation parent population artificial breeding, realizes artificial seedling rearing.The present invention provides a kind of modes for being suitble to half to pierce thick lip fry kind large-scale production.The technological break-through because half pierce thick lip fish Wild ornamental resources it is deficient due to caused by parent population source difficulty bottleneck; thick lip fish national level Fish Germplasm Resources protection zone is pierced for half and other wild resource reparations for having Natural Population to be distributed waters provide technical support and seed guarantee, and can realize scale breeding and the artificial breeding industrialization of half thorn thickness lip fish.
Description
Technical field
The invention belongs to field aquaculture fields, and in particular to a kind of half pierces thick lip fish artificial seedling rearing's method.
Background technique
At present China fresh water original inhabitants' characteristic fish species protection in terms of research work make slow progress, base
Plinth is relatively weak.In recent years, many fresh water original inhabitants kinds with certain research and development value are due to source area ecological environment day
Benefit deteriorates, and fishery output is greatly reduced；In addition the research of its artificial breeding technology, nutritional need and cultural technique etc. is still
It does not break through, supply falls short of demand for marketable fish in the market, and the phenomenon that being draind the pond to get all the fish by excessively capturing of driving of economic interests etc. even more prohibits not repeatly
Only, lead to the population quantity and the serious failure of quality of these indigenous kinds, Wild ornamental resources have the risk of extinction.In view of
This, it is necessary to the research work such as numerous and cultivation exploitation are expanded to the distinctive fresh water original inhabitants kind in China development protection of resources, seed, with
The recovery and continuity for realizing its Wild ornamental resources, meet the market demand.
The thick lip fish of half thorn is that " Nanpu small stream half pierces thick lip fish national level Fish Germplasm Resources protection zone " (Ministry of Agriculture criticizes within 2015
Standard is established) and " Songxi thickness lip fish national level Fish Germplasm Resources protection zone " (2016 the Ministry of Agriculture approval foundation) main protection
Species are Fujian Zhejiang and Jiangxi one with common food fish, and deep to be liked by common people, wide market has the important ecological value
And nutritive value.
For various reasons, the correlative study data in relation to the thick lip fish of half thorn is few, fundamental biological knowledge, breeding biology
The research of, aquaculture organism, nutrition and disease Control Technology etc. is substantially at blank；Artificial breeding is rarely seen Liu Li
Thick lip fish parent is pierced using raise and train wild half within beautiful equal (2010), carried out the test of its artificial breeding in 2008~2009 years,
Obtain a small amount of seed.In consideration of it, realizing good spawning effect present invention employs new oxytocic drug compatibility；It is solving
It has determined after stable sufficient provisioning technique, seed and the Cultivating techniques of parent population of biological feed, has formd stable seed rearing skill
Art realizes the artificial seedling rearing of half thorn thickness lip fish, pierces thick lip fry kind large-scale production and its protection of germ plasm resource for half
Solid foundation has been established with cultivation development and application.
Summary of the invention
It is an object of the present invention to pierce the status that thick lip fingerling seedling Cultivating techniques lack for above-mentioned half, a kind of half is provided
Pierce thick lip fish artificial seedling rearing's method.
For achieving the above object, the present invention adopts the following technical scheme:
A kind of half pierces thick lip fish artificial seedling rearing's method, the specific steps of which are as follows:
(1) parent culture:
(a) daily cultivation: the thick lip fish wild type of half thorn being put in a suitable place to breed respectively and carries out acclimatization culture in Yu Tuchi, breeding density
3~5 tails/m2, another intercropping silver carp bighead is to regulate and control water quality；It is cultivated using the artifical compound feed of protein content 33%~36%,
Day feeds 2 times, and day feeding volume is the 0.5%~1% of fish body weight；
Further, the Tu Chi is area in 2000 m2Within, 1.0~1.5 m of the depth of water, it is ensured that miniflow water；
(b) antenatal reinforced cultivating: in season in the beginning of spring, the good parent of gonad development is selected from parent fish pond in batches, is transferred to water
Mud sump carries out reinforced cultivating, and each cement pit launches 200~300 tail of parent population, keeps 3~5 h/d of pond bath；In artificial induced spawning
Preceding 10~15 d ensures 8 h/d or more of flushing period, to promote parent population gonad development；Using the artificial of protein content 36%~40%
Mixed feed and greenfeed are cultivated, and feed 2~3 mixed feed day, 1%~2% that day feeding volume is fish body weight, greenfeed
It feeds every other day 1 time；
Further, the cement pit is 40 m of area2~60 m2, the rectangle cement pit of 0.5~0.8 m of the depth of water, and
It is equipped with one, 1.5 kw water pump, keeps miniflow water, water inlet is filtered with 80~100 mesh silks；
(2) artificial induced spawning:
The healthy and strong individual without wound, gonadal maturation is selected from the parent population of reinforced cultivating and carries out artificial induced spawning test: being urged
It produces hormone and uses luteotropin releasing hormone d-ala analog (LRH-A2)+maleic acid dioxone (DOM)+human chorionic gonadtropin
(HCG) mix preparation, 2 needle injection systems；1st needle injects oxytocic drug: 3 mg LRH-A2+4 mg DOM+100 IU HCG)；
2nd needle injects oxytocic drug: 5 mg LRH-A2+5 mg DOM+1000 IU HCG)；10~12 h of needle gage, milter do not inject
1 needle, the 2nd injection amount are the 1/2 of raun；Under 23 DEG C~27 DEG C water temperature conditions, Effect time is 10~14 h；Female-male proportion is
3~5:1；
(3) artificial insemination: artificial insemination uses dry method insemination method；
(4) artificial incubation:
Fertilized eggs after water swelling are evenly arranged on hatching cloth ovum frame, in 25 DEG C~27 DEG C hatching water temperature conditions
Under, prelarva is about through 58~60 h membranes；3 age in days prolarvas artificially not startle；Ensure to hatch water body dissolved oxygen in hatching process to exist
5 mg/l or more；Hatching pattern is using micro- Lotic hatching or the inflation hatching of miniflow water；
(5) seed early period cultivates:
(a) open-mouthed bait is cultivated: with 2000 m of area2Within, the Tu Chi of 0.6 ~ 0.8 m of the depth of water is bait culture pond,
What preceding 8~12 d for starting artificial induced spawning work carried out bait culture pond levels work；7~9 d after cleaning up the pond, disappears to drug toxicity
It loses, injects new water to 0.5~0.6 m of the depth of water；Every 667 m280~100 kg of organic fertilizer to ferment is applied, is swum with culture dynamic
Plant；
(b) larvae cultivation: feeding the zooplankter that 80 mesh in above-mentioned bait culture pond are sieved before 10 age in days of prelarva, and 10~25
Age in days then feeds the zooplankter being sieved with 60 mesh, and the hand fit that 20 ages in days start to tame and docile food protein content 40%~45% raises
Material；Cultivation of the prelarva through 39~51 d can be obtained specification up to the juvenile fish of 3 cm or more, which is summer flower seed；
(6) first filial generation large size seedling seed is cultivated:
The first filial generation juvenile fish clear water pond-inputting of 3~5 cm of specification is transferred to Tu Chi progress large size seedling seed cultivation and is formed；Day
It feeds mixed feed 2~3 times, day feeding volume is the 3%~5% of fish body weight, which is protein content 38%~42%
Heavy property particulate material；When most of first filial generation seed size is up to 10 cm, by seed harvesting sizing screening, 10 cm seed below
Continue to stay in Tu Chi cultivation, the seed of 10 cm or more is transferred to other Tu Chi, carries out standby parent culture；
Further, the native pool area is in 2000 m2Within, 0.8~1.0 cm of the depth of water, miniflow water, water inlet with 60~
The filtering of 80 mesh silks；
(7) first filial generation parent forms and cultivates:
(a) standby parent in first filial generation forms: it feeds day mixed feed 2~3 times, day feeding volume is the 2%~3% of fish body weight,
The mixed feed is the heavy property particulate material of protein content 36%~40%；To most of first filial generation standby parent specification up to 15 cm
When, its harvesting is subjected to second of sizing screening, the standby parent of 15 cm or more of screening is transferred to other Tu Chi, carries out son one
For parent culture；
Further, parent culture pond is Tu Chi described in step (6)；
(b) first filial generation parent culture: the same step of breeding method (1)；
(8) realization of artificial seedling rearing: obtaining the first filial generation parent of gonadal maturation using step (1) operating method,
It repeats step (2) to step (4), obtains second filial seed, realize artificial seedling rearing.
The concrete operation step of dry method insemination method described in step (3) are as follows: will be wiped to the raun abdomen moisture of effector phase
It is dry, gently press its abdomen to squeeze into ovum in dry container from gonopore；The essence squeezed out simultaneously with suction pipe from the sucking of milter gonopore
Liquid with pouring into freshly extruded ovum after 0.7% normal saline dilution of 1~2 times of volume pH 7.0, then is gently mixed with feather mixed
After even 2~3 min, fertilized eggs are cleaned and go to glue by addition clear water, are then hatched.
The beneficial effects of the present invention are:
Half, which pierces thick lip fish, has the important ecological value and nutritive value, in recent years since source area ecological environment is increasingly disliked
Change, the serious failure of population quantity and quality of Wild ornamental resources.Present invention employs new oxytocic drug compatibilities, realize
Good spawning effect；Solve biological feed stablizes sufficient provisioning technique (a kind of novel zooplankter acquisition device, patent
Number: ZL201520003941.0), the Cultivating techniques of seed and parent population, stable rearing of fingerling is formd, at home outside
The secondary artificial seedling rearing for realizing half thorn thickness lip fish breaches parent population caused by due to the thick lip fish Wild ornamental resources of half thorn are deficient
The bottleneck of source difficulty, piercing thick lip fish national level Fish Germplasm Resources protection zone and other for half has Natural Population to be distributed waters
Wild resource reparation provides technical support and seed ensures, and can realize scale breeding and the artificial breeding of half thorn thickness lip fish
Industrialization.
Specific embodiment
Embodiment 1
Training is utilized with the method for the present invention in the emerging fingerling in Shunchang, Fujian Province million cultivation Co., Ltd 4~June in 2015
Half educated pierces thick lip fish wild type, has successfully carried out its artificial breeding.It hastens parturition altogether 6 batches, total 227 tail of raun of hastening parturition,
It hastens parturition successfully 187 tail, the mean spawning rate is up to 82.4 %；Obtain 16.55 ten thousand, ovum, fertilized eggs 11.8 ten thousand, average rate of fertilization
71.4 %；Hatch 79660 tail of spray seedling, 67.4 % of average percentage hatch rate.By the cultivation of 40~51 d, average size is obtained altogether
The half of 3.37 cm pierces thick 62630 tail of lip fish summer flower seed, and seed rearing average viability reaches 78.6 %.Its specific breeding step
Suddenly are as follows:
(1) parent culture:
(a) daily cultivation: the thick lip fish wild type of half thorn being put in a suitable place to breed respectively and carries out acclimatization culture in Yu Tuchi, breeding density
3 tails/m2, another intercropping silver carp bighead is to regulate and control water quality；It is cultivated using the artifical compound feed of protein content 33%, day feeds
2 times, day feeding volume is the 0.5% of fish body weight；
Further, it is 1000 m that the Tu Chi, which is area,2, 1.0 m of the depth of water, miniflow water；
(b) antenatal reinforced cultivating: in season in the beginning of spring, the good parent of gonad development is selected from parent fish pond in batches, is transferred to water
Mud sump carries out reinforced cultivating, and each cement pit launches 200 tail of parent population, keeps 3 h/d of pond bath；10 d are true before artificial induced spawning
8 h/d of flushing period is protected, to promote parent population gonad development；It is carried out using the artifical compound feed and greenfeed of protein content 36%
It cultivates, feeds 2 mixed feed day, 1% that day feeding volume is fish body weight, greenfeed is fed 1 time every other day；
Further, the cement pit is 40 m of area2, the rectangle cement pit of 0.5 m of the depth of water, and it is equipped with 1.5 kw water
Pump one, keeps miniflow water, and water inlet is filtered with 60 mesh silks；
(2) artificial induced spawning:
The healthy and strong individual without wound, gonadal maturation is selected from the parent population of reinforced cultivating and carries out artificial induced spawning test: being urged
It produces hormone and uses luteotropin releasing hormone d-ala analog (LRH-A2)+maleic acid dioxone (DOM)+human chorionic gonadtropin
(HCG) mix preparation, 2 needle injection systems；1st needle injects oxytocic drug: 3 mg LRH-A2+4 mg DOM+100 IU HCG)；
2nd needle injects oxytocic drug: 5 mg LRH-A2+5 mg DOM+1000 IU HCG)；12 h of needle gage, milter do not inject the 1st needle,
2nd injection amount is the 1/2 of raun；Under 23 DEG C~25 DEG C of water temperature condition, the hormone effect time is 12~14 h；Female-male proportion
For 3:1；
(3) artificial insemination: artificial insemination uses dry method insemination method；
(4) artificial incubation:
Fertilized eggs after water swelling are evenly arranged on hatching cloth ovum frame, under 25 DEG C of hatching water temperature conditions, prelarva
About through 60 h membranes；3 age in days prolarvas artificially not startle；Ensure to hatch water body dissolved oxygen in hatching process in 5 mg/l；Hatching
Mode uses micro- Lotic hatching；
(5) seed early period cultivates:
(a) open-mouthed bait is cultivated: with 1000 m of area2, the Tu Chi of 0.6 m of the depth of water is bait culture pond, is being started manually
Hasten parturition work preceding 8 d carry out bait culture pond level work；7 d after cleaning up the pond disappears to drug toxicity, injects new water to water
Deep 0.5 m；Every 667 m280 kg of organic fertilizer to ferment is applied, to cultivate plankton；
(b) larvae cultivation: feeding the zooplankter that 80 mesh in above-mentioned bait culture pond are sieved before 10 age in days of prelarva, and 10~25
Age in days then uses 60 mesh to be sieved, and 20 ages in days start to tame and docile the artifical compound feed of food protein content 40%, which feeds heavy property particle
Material；Cultivation of the prelarva through 40~51 d can be obtained specification up to the juvenile fish of 3 cm or more, which is summer flower seed；
(6) first filial generation large size seedling seed is cultivated:
The first filial generation juvenile fish clear water pond-inputting of 3 cm of specification is transferred to Tu Chi progress large size seedling seed cultivation and is formed；Day feeds
Mixed feed 2 times, day feeding volume is the 3% of fish body weight, which is the heavy property particulate material of protein content 38%；To big portion
When molecule generation seed size is up to 10 cm, by seed harvesting sizing screening, 10 cm seed below continues to cultivate in Tu Chi, and 10
The seed of cm or more is transferred to other Tu Chi, carries out standby parent culture；
Further, the native pool area is 1000 m2, 0.8 m of the depth of water, holding miniflow water, 60 mesh silks of intaking
Filtering；
(7) first filial generation parent forms and cultivates:
(a) standby parent in first filial generation forms: feeding day mixed feed 2 times, day feeding volume is the 2% of fish body weight, which raises
Material is the heavy property particulate material of protein content 36%；When most of first filial generation standby parent specification is up to 15 cm, by its harvesting into
The standby parent of second of sizing screening of row, 15 cm or more of screening is transferred to other Tu Chi, carries out first filial generation parent culture；
Further, parent culture pond is Tu Chi described in step (6)；
(b) first filial generation parent culture: the same step of breeding method (1)；
The concrete operation step of dry method insemination method described in step (3) are as follows: will be wiped to the raun abdomen moisture of effector phase
It is dry, gently press its abdomen to squeeze into ovum in dry container from gonopore；The essence squeezed out simultaneously with suction pipe from the sucking of milter gonopore
Liquid with pouring into freshly extruded ovum after 0.7% normal saline dilution of 1 times of volume pH 7.0, then is gently mixed mixing 2 with feather
After min, fertilized eggs are cleaned and go to glue by addition clear water, are then hatched.
Embodiment 2
5~June in 2016, Co., Ltd is cultivated in the emerging fingerling in Shunchang, Fujian Province million, pierces thick lip fish using the half of cultivation
Wild type has carried out the work of its artificial breeding with the method for the present invention.It has hastened parturition altogether 5 batches, 452 tail of raun of hastening parturition is urged
Yield 77.2%；Obtain 19.33 ten thousand, ovum, fertilized eggs 150,000, rate of fertilization 77.6%；Obtain 11.8 ten thousand tail of spray seedling, hatching
Rate 78.7%.By the cultivation of 39~48 d, the half of 3.16 cm of average size is obtained altogether and pierces thick 77500 tail of lip fish summer flower seed,
Seed rearing average viability reaches 65.7 %.
The specific steps of which are as follows:
(1) parent culture:
(a) daily cultivation: the thick lip fish wild type of half thorn being put in a suitable place to breed respectively and carries out acclimatization culture in Yu Tuchi, breeding density
4 tails/m2, another intercropping silver carp bighead is to regulate and control water quality；It is cultivated using the artifical compound feed of protein content 35%, day feeds 3
Secondary, day feeding volume is the 0.8% of fish body weight；
Further, the native pool area is 2000 m2, 1.2 m of the depth of water, holding miniflow water；
(b) antenatal reinforced cultivating: in season in the beginning of spring, the good parent of gonad development is selected from parent fish pond in batches, is transferred to water
Mud sump carries out reinforced cultivating, and each cement pit launches 250 tail of parent population, keeps 4 h/d of pond bath；12 d are true before artificial induced spawning
10 h/d of flushing period is protected, to promote parent population gonad development；It is carried out using the artifical compound feed and greenfeed of protein content 38%
It cultivates, feeds 3 mixed feed day, 1.5% that day feeding volume is fish body weight, greenfeed is fed 1 time every other day；
Further, the cement pit is 50 m of area2, the rectangle cement pit of 0.6 m of the depth of water, and it is equipped with 1.5 kw water
Pump one, keeps miniflow water, and water inlet is filtered with 80 mesh silks；
(2) artificial induced spawning:
The healthy and strong individual without wound, gonadal maturation is selected from the parent population of reinforced cultivating and carries out artificial induced spawning test: being urged
It produces hormone and uses luteotropin releasing hormone d-ala analog (LRH-A2)+maleic acid dioxone (DOM)+human chorionic gonadtropin
(HCG) mix preparation, 2 needle injection systems；1st needle injects oxytocic drug: 3 mg LRH-A2+4 mg DOM+100 IU HCG)；
2nd needle injects oxytocic drug: 5 mg LRH-A2+5 mg DOM+1000 IU HCG)；11 h of needle gage, milter do not inject the 1st needle,
2nd injection amount is the 1/2 of raun；Under 25 DEG C~27 DEG C of water temperature condition, the hormone effect time is 10~12 h.Female-male proportion
For 4:1；
(3) artificial insemination: artificial insemination uses dry method insemination method；
(4) artificial incubation:
Fertilized eggs after water swelling are evenly arranged on hatching cloth ovum frame, under 26 DEG C of hatching water temperature conditions, prelarva
About through 59 h membranes；3 age in days prolarvas artificially not startle；Ensure to hatch water body dissolved oxygen in hatching process in 5 mg/l or more；
Hatching pattern is using micro- Lotic hatching or the inflation hatching of miniflow water；
(5) seed early period cultivates:
(a) open-mouthed bait is cultivated: with 2000 m of area2, the Tu Chi of 0.7 m of the depth of water is bait culture pond, is being started manually
Hasten parturition work preceding 10 d carry out bait culture pond level work；8 d after cleaning up the pond disappears to drug toxicity, injects new water extremely
0.6 m of the depth of water；Every 667 m290 kg of organic fertilizer to ferment is applied, to cultivate plankton；
(b) larvae cultivation: feeding the zooplankter that 80 mesh in above-mentioned bait culture pond are sieved before 10 age in days of prelarva, and 10~25
Age in days then uses 60 mesh to be sieved, and 20 ages in days start to tame and docile the artifical compound feed of food protein content 43%, which feeds heavy property particle
Material；Cultivation of the prelarva through 39~50 d can be obtained specification up to the juvenile fish of 3 cm or more, which is summer flower seed；
(6) first filial generation large size seedling seed is cultivated:
The first filial generation juvenile fish clear water pond-inputting of specification 4cm is transferred to Tu Chi progress large size seedling seed cultivation and is formed；Day feeds
Mixed feed 2 times, day feeding volume is the 4% of fish body weight, which is the heavy property particulate material of protein content 40%；To big portion
When molecule generation seed size is up to 10 cm, by seed harvesting sizing screening, 10 cm seed below continues to cultivate in Tu Chi, and 10
The seed of cm or more is transferred to other Tu Chi, carries out standby parent culture；
Further, the native pool area is 2000 m2, 0.9 m of the depth of water, holding miniflow water, 80 mesh silks of intaking
Filtering；
(7) first filial generation parent forms and cultivates:
(a) standby parent in first filial generation forms: feeding day mixed feed 3 times, day feeding volume is the 2.5% of fish body weight, the cooperation
Feed is the heavy property particulate material of protein content 38%；When most of first filial generation standby parent specification is up to 15 cm, by its harvesting
Second of sizing screening is carried out, the standby parent of 15 cm or more of screening is transferred to other Tu Chi, carries out first filial generation parent culture；
Further, parent culture pond is Tu Chi described in step (6)；
(b) first filial generation parent culture: the same step of breeding method (1)；
The concrete operation step of dry method insemination method described in step (3) are as follows: will be wiped to the raun abdomen moisture of effector phase
It is dry, gently press its abdomen to squeeze into ovum in dry container from gonopore；The essence squeezed out simultaneously with suction pipe from the sucking of milter gonopore
Liquid with pouring into freshly extruded ovum after 0.7% normal saline dilution of 2 times of volume pH 7.0, then is gently mixed mixing with feather
After 2.5 min, fertilized eggs are cleaned and go to glue by addition clear water, are then hatched.
Embodiment 3
On June 21st, 2016, using 2014 from parent made of numerous first filial generation seed rearing, by artificial induced spawning,
Ovum insemination is squeezed, acquisition first filial generation parent oviposition is 31000 sub, and fertilized eggs 7600, rate of fertilization 24.5%；Obtain spray seedling 2300
Tail, hatching rate 30.3% realize the artificial seedling rearing of half thorn thickness lip fish.Its specific breeding step are as follows:
(1) parent culture:
(a) daily cultivation: the thick lip fish wild type of half thorn being put in a suitable place to breed respectively and carries out acclimatization culture in Yu Tuchi, breeding density
5 tails/m2, another intercropping silver carp bighead is to regulate and control water quality；It is cultivated using the artifical compound feed of protein content 36%, day feeds 3
Secondary, day feeding volume is the 1% of fish body weight；
Further, the native pool area is 1000 m2, 1.5 m of the depth of water, holding miniflow water；
(b) antenatal reinforced cultivating: in season in the beginning of spring, the good parent of gonad development is selected from parent fish pond in batches, is transferred to water
Mud sump carries out reinforced cultivating, and each cement pit launches 300 tail of parent population, keeps 5 h/d of pond bath；15 d are true before artificial induced spawning
10 h/d of flushing period is protected, to promote parent population gonad development；It is carried out using the artifical compound feed and greenfeed of protein content 40%
It cultivates, feeds 3 mixed feed day, 2% that day feeding volume is fish body weight, greenfeed is fed 1 time every other day；
Further, the cement pit is 60 m of area2, the rectangle cement pit of 0.8 m of the depth of water, and it is equipped with 1.5 kw water
Pump one, keeps miniflow water, and water inlet is filtered with 100 mesh silks；
(2) artificial induced spawning:
The healthy and strong individual without wound, gonadal maturation is selected from the parent population of reinforced cultivating and carries out artificial induced spawning test: being urged
It produces hormone and uses luteotropin releasing hormone d-ala analog (LRH-A2)+maleic acid dioxone (DOM)+human chorionic gonadtropin
(HCG) mix preparation, 2 needle injection systems；1st needle injects oxytocic drug: 3 mg LRH-A2+4 mg DOM+100 IU HCG)；
2nd needle injects oxytocic drug: 5 mg LRH-A2+5 mg DOM+1000 IU HCG)；10 h of needle gage, milter do not inject the 1st needle,
2nd injection amount is the 1/2 of raun；Under 25 DEG C~27 DEG C of water temperature condition, the hormone effect time is 10~12 h；Female-male proportion
For 5:1；
(3) artificial insemination: artificial insemination uses dry method insemination method；
(4) artificial incubation:
Fertilized eggs after water swelling are evenly arranged on hatching cloth ovum frame, under 27 DEG C of hatching water temperature conditions, prelarva
About through 58 h membranes；3 age in days prolarvas artificially not startle；Ensure to hatch water body dissolved oxygen in hatching process in 5 mg/l or more；
Hatching pattern uses micro- Lotic hatching；
(5) seed early period cultivates:
(a) open-mouthed bait is cultivated: with 2000 m of area2, the pond of 0.8 m of depth of water bait culture pond the most, in beginning people
Work hasten parturition work preceding 12 d carry out bait culture pond level work；9 d after cleaning up the pond disappears to drug toxicity, injects new water
To 0.6 m of the depth of water；Every 667 m2100 kg of organic fertilizer to ferment is applied, to cultivate plankton；
(b) larvae cultivation: feeding the zooplankter that 80 mesh in above-mentioned bait culture pond are sieved before 10 age in days of prelarva, and 10~25
Age in days then uses 60 mesh to be sieved, and 20 ages in days start to tame and docile the artifical compound feed of food protein content 45%, which feeds heavy property particle
Material；Cultivation of the prelarva through 39~48 d can be obtained specification up to the juvenile fish of 3 cm or more, which is summer flower seed；
(6) first filial generation large size seedling seed is cultivated:
The first filial generation juvenile fish clear water pond-inputting of 3 cm of specification is transferred to Tu Chi progress large size seedling seed cultivation and is formed；Day feeds
Mixed feed 3 times, day feeding volume is the 5% of fish body weight, which is the heavy property particulate material of protein content 42%；To big portion
When molecule generation seed size is up to 10 cm, by seed harvesting sizing screening, 10 cm seed below continues to cultivate in Tu Chi, and 10
The seed of cm or more is transferred to other Tu Chi, carries out standby parent culture；
Further, the native pool area is 2000 m2, 1.0 m of the depth of water, holding miniflow water, 80 mesh silks of intaking
Filtering；
(7) first filial generation parent forms and cultivates:
(a) standby parent in first filial generation forms: feeding day mixed feed 3 times, day feeding volume is the 3% of fish body weight, which raises
Material is the heavy property particulate material of protein content 40%；When most of first filial generation standby parent specification is up to 15 cm, by its harvesting into
The standby parent of second of sizing screening of row, 15 cm or more of screening is transferred to other Tu Chi, carries out first filial generation parent culture；
Further, parent culture pond is Tu Chi described in step (6)；
(b) first filial generation parent culture: the same step of breeding method (1)；
(8) realization of artificial seedling rearing: obtaining the first filial generation parent of gonadal maturation using step (1) operating method,
It repeats step (2) to step (4), obtains second filial seed, realize artificial seedling rearing.
The concrete operation step of dry method insemination method described in step (3) are as follows: will be wiped to the raun abdomen moisture of effector phase
It is dry, gently press its abdomen to squeeze into ovum in dry container from gonopore；The essence squeezed out simultaneously with suction pipe from the sucking of milter gonopore
Liquid with pouring into freshly extruded ovum after 0.7% normal saline dilution of 2 times of volume pH 7.0, then is gently mixed mixing 3 with feather
After min, fertilized eggs are cleaned and go to glue by addition clear water, are then hatched.
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, is all covered by the present invention.
Claims (2)
1. a kind of half pierces thick lip fish artificial seedling rearing's method, which is characterized in that the specific steps of which are as follows:
(1) parent culture:
(a) daily cultivation: the thick lip fish wild type of half thorn being put in a suitable place to breed respectively and carries out acclimatization culture in Yu Tuchi, breeding density 3~5
Tail/m2, another intercropping silver carp bighead is to regulate and control water quality；It is cultivated using the artifical compound feed of protein content 33%~36%, day throws
It feeds 2 times, day feeding volume is the 0.5%~1% of fish body weight；
Further, the native pool area is in 2000 m2Within, 1.0~1.5 m of the depth of water, keep miniflow water；
(b) antenatal reinforced cultivating: in season in the beginning of spring, the good parent of gonad development is selected from parent fish pond in batches, is transferred to cement pit
Reinforced cultivating is carried out, each cement pit launches 200~300 tail of parent population, keeps 3~5 h/d of pond bath；10 before artificial induced spawning~
15 d ensure 8 h/d or more of flushing period, to promote parent population gonad development；It is raised using the hand fit of protein content 36%~40%
Material and greenfeed are cultivated, and feed 2~3 mixed feed day, 1%~2% that day feeding volume is fish body weight, greenfeed is thrown every other day
It feeds 1 time；
Further, the cement pit is 40 m of area2~60 m2, 0.5~0.8 m of the depth of water rectangle cement pit, and be equipped with
1.5 one, kw water pumps, keep miniflow water, and water inlet is filtered with 80~100 mesh silks；
(2) artificial induced spawning:
The healthy and strong individual without wound, gonadal maturation is selected from the parent population of reinforced cultivating and carries out artificial induced spawning test: being hastened parturition sharp
Element uses luteotropin releasing hormone d-ala analog LRH-A2+ maleic acid dioxone (DOM)+human chorionic gonadtropin (HCG) is mixed
Close preparation, 2 needle injection systems；1st needle injects oxytocic drug: 3 mg LRH-A2+4 mg DOM+100 IU HCG；2nd needle note
Penetrate oxytocic drug: 5 mg LRH-A2+5 mg DOM+1000 IU HCG；10~12 h of needle gage, milter do not inject the 1st needle, and the 2nd
Injection amount is the 1/2 of raun；Under 23 DEG C~27 DEG C water temperature conditions, Effect time is 10~14 h；Female-male proportion is 3~5:1；
(3) artificial insemination: artificial insemination uses dry method insemination method；
(4) artificial incubation:
Fertilized eggs after water swelling are evenly arranged on hatching cloth ovum frame, it is young under 25 DEG C~27 DEG C hatching water temperature conditions
Fish is about through 58~60 h membranes；3 age in days prolarvas artificially not startle；Ensure to hatch water body dissolved oxygen in hatching process in 5 mg/
L or more；Hatching pattern is using micro- Lotic hatching or the inflation hatching of miniflow water；
(5) seed early period cultivates:
(a) open-mouthed bait is cultivated: with 2000 m of area2Within, the Tu Chi of 0.6~0.8 m of the depth of water be bait culture pond, starting
What preceding 8~12 d of artificial induced spawning work carried out bait culture pond levels work；7~9 d after cleaning up the pond disappears to drug toxicity,
New water is injected to 0.5~0.6 m of the depth of water；Every 667 m280~100 kg of organic fertilizer after application fermentation is swum to move with culture and be planted
Object；
(b) zooplankter that 80 mesh are sieved in above-mentioned bait culture pond, 10~25 ages in days larvae cultivation: are fed before 10 age in days of prelarva
The zooplankter being sieved with 60 mesh is then fed, 20 ages in days start to tame and docile the artifical compound feed of food protein content 40%~45%；It is young
Cultivation of the fish through 39~51 d can be obtained specification up to the juvenile fish of 3 cm or more, which is summer flower seed；
(6) first filial generation large size seedling seed is cultivated:
The first filial generation juvenile fish clear water pond-inputting of 3~5 cm of specification is transferred to Tu Chi progress large size seedling seed cultivation and is formed；Day feeds
Mixed feed 2~3 times, day feeding volume is the 3%~5% of fish body weight, which is the heavy property of protein content 38%~42%
Particulate material；When most of first filial generation seed size is up to 10 cm, by seed harvesting sizing screening, 10 cm seed below continues
Tu Chi cultivation is stayed in, the seed of 10 cm or more is transferred to other Tu Chi, carries out standby parent culture；
Further, the native pool area is in 2000 m2Within, 0.8~1.0 m of the depth of water, miniflow water, 60~80 meshes of intaking
Silk filtering；
(7) first filial generation parent forms and cultivates:
(a) standby parent in first filial generation forms: feeding day mixed feed 2~3 times, day feeding volume is the 2%~3% of fish body weight, this is matched
Close the heavy property particulate material that feed is protein content 36%~40%；It, will when most of first filial generation standby parent specification is up to 15 cm
Its harvesting carries out second of sizing screening, and the standby parent of 15 cm or more of screening is transferred to other Tu Chi, carries out first filial generation parent
It cultivates；
Further, parent culture pond is Tu Chi described in step (6)；
(b) first filial generation parent culture: the same step of breeding method (1)；
(8) realization of artificial seedling rearing: obtaining the first filial generation parent of gonadal maturation using step (1) operating method, repeats
Step (2) obtains second filial seed, realizes artificial seedling rearing to step (4).
2. according to claim 1 a kind of half pierces thick lip fish artificial seedling rearing's method, which is characterized in that step (3) is described
Dry method insemination method concrete operation step are as follows: will be dried to the raun abdomen moisture of effector phase, gently press its abdomen by ovum from
Gonopore is squeezed into dry container；The sperm squeezed out simultaneously with suction pipe from the sucking of milter gonopore, with 1 ~ 2 times of volume pH 7.0
0.7% normal saline dilution after pour into freshly extruded ovum, then after being gently mixed 2~3 min of mixing with feather, clear water is added
Fertilized eggs are cleaned and go to glue, are then hatched.
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