CN106879509A - A kind of bar house oil fish artificial fecundation method - Google Patents
A kind of bar house oil fish artificial fecundation method Download PDFInfo
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- CN106879509A CN106879509A CN201710180309.7A CN201710180309A CN106879509A CN 106879509 A CN106879509 A CN 106879509A CN 201710180309 A CN201710180309 A CN 201710180309A CN 106879509 A CN106879509 A CN 106879509A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
It is contemplated that a kind of bar house oil fish artificial fecundation method is disclosed, using following steps：Set up fishpond, fish body sterilization, hot housing adult fish, selection parent population, injection ocyodinic, management spawning before parent population, artificial insemination, artificial incubation, cultivate seed; it is slow that the present invention solves bar house oil fish gonad development; female, the nonsynchronous method of milter gonad development; and propose fry opening material and instruct feeding for feed, seedling can be provided for large-scale cultivation bar house oil fish.
Description
Technical field
The invention belongs to cultural technique field, more particularly to a kind of bar house oil fish artificial fecundation method.
Background technology
Bar house oil fish original name bar horse Sui lips Barb（Crossocheilus bamaensis Fang,sp.nov.）, bar horse intend tassel
Fish（Pseudocrossocheilus bamaensisFang）, main product in the Pan Yanghe in Guangxi China bar horse border of the county, and
Hongsuihe River mainstream, is the Guangxi rivers rare small-sized economic fish of original inhabitants.Its delicious meat, edibility are high and with one
Fixed medical value, is described as " ginseng under water ", is one of township Bama County of Guangxi " bar horse Triratna " of Chinese Famous longevity.
The requirement that bar house oil fish is perched, survived, grown and bred to environmental condition is special, propagates middle gonad development artificially
Slowly, female, milter gonad development is asynchronous, and scale artificial culture cannot be carried out so far.Sell the current edible fish market of bar house oil fish
Valency has price but no buyers in 200 yuan/more than kg（Fish）.The vigorous market demand, urgent need is carried out its large-scale artificial and is bred, and is passed through
The popularization cultivation of artificial breeding seed, alleviates its market supply and demand contradiction.Since nineteen eighty-two, multiple departments successively carry out in Guangxi District
The Primary Study experiment to bar house oil fish related, but still fail to break through its large-scale artificial breeding key technology, seafood so far
In the market bar house oil fish culture product is still in blank stage.Caught plus manually excessively capturing for many years, stealing, rivers fish germ plasm resource connects
It is near exhausted.Carry out bar house oil fish artificial domesticating and cultivating and biological control technical research, artificial breeding nursery is supplied to social batch
Kind, help to drive the development of bar house oil fish culture industry and Poor Mountainous Area peasant cultivation to get rich, if meeting national at present and following
The estate planning in dry year and supply side reform orientation；To realizing bar house oil fish enhancement releasing, protection River Fish resource, promoting bar
The industrialization of house oil fish and the development of local tour tourism business have important meaning view.Not yet there is bar house oil fish artificial propagation success at present
Case, it is slow that the present invention solves bar house oil fish gonad development, female, the nonsynchronous method of milter gonad development, and solves fish
The mode that feeds of seedling opening material and instruction feed belongs to national pioneering.
The content of the invention
It is an object of the invention to provide a kind of bar house oil fish artificial fecundation method, bar house oil fish gonad development is solved
Slowly, female, the nonsynchronous method of milter gonad development, and solve the mode that feeds of fry opening material and handling feed and belong to the whole nation
It is pioneering.Implementation of the invention can provide the domestication method of seedling and fry for large-scale cultivation bar house oil fish.
The present invention is achieved by the following technical solutions：
A kind of bar house oil fish artificial fecundation method：
（1）The foundation in fishpond：
Selection builds fishpond in Bama County of Guangxi or periphery mimic panel sun Ha Noi environment, and bottom of pond lays one layer of pebble, and does
Good fishpond and the disinfection of pebble, the soft fishnet in fishpond are divided into two pieces of isolated areas, it is ensured that female milter is in same water
Body, separates again each other, and shading.It is finally putting into the mountain spring water and subterranean stream with the disk close water of sun quality of river water or cleaning
Water, parent fish pond will possess flowing water flow in pipes and irrigation and drainage are convenient, manually manufacture current, use stimulation by running water parent population.
（2）Fish body is sterilized：
Fish body is carried out disinfection using pure Chinese medicinal preparation FUYINJIE washing lotion, Chinese medicine preparation FUYINJIE washing lotion concentration is 250 milliliters/it is vertical
Square meter Shui Ti, temperature control is in 18-22 °C, water-bath 1 hour, period simultaneously uninterrupted oxygenation.
（3）Adult fish hot housing：
Health in vigorous health, appearance is selected normally without the 2-3 age bar house oil fish male and female parent populations for abrading and reaching maturity, to disinfect
The flowing water that has that bar house oil fish male and female parent population is respectively put into dictyosome segmentation is injected with the convenient culture pond of irrigation and drainage, and male and female parent population was both
It is again separated from one another in a water body, the special group's of the sinking to the bottom material bait of kneading is fed daily twice, the excrement of bottom of pond is at least extracted daily
Just and water is changed once, quantity of exchanged water is the 1/3 of water；Water temperature is down to 10 °C with cooling-water machine, with cold stimulation by running water, is spaced daily
Starting within 3 hours flowing cool water stimulates 1 hour, then warms naturally to normal temperature.Articial bait making is using protein content 43%
Eel special compound feed sinks to the bottom a material, vitamin E or zinc gluconate and Selenium in Plants protein-enrichmen culture is added, until bar horse
Oily fish reach artificial insemination needed for maturity.Such as milter maturity not enough, seminal fluid cannot be manually extruded, also can be by milter list pond
Individually culture, in addition to expecting by above feed formula and the kneading group of sinking to the bottom, can be individually added into Chinese medicine preparation wuzi yanzong pills or production of sperm
The medicine that soup etc. promotees patrogenesis accelerates the ripening milter sperm.Before artificial insemination, clomiphene is added in feed, female milter presses fish simultaneously
Body 5mg/kg admixed and continuously fed 5 days in feed.
（4）The selection of parent population
Annual 4-7 months, physique healthy and strong, disease-free without wound is selected, the good parent population of gonad development carries out artificial induced spawning；Sexually matured bar
Horse becomes yellow and deepens by original yellow green, female, male to be easier to differentiate；Raun belly expands, and accidental ovary profile, cloacal aperture is micro-
It is red, belly is touched, feel has certain full, pliability；Substantially, hand touches with obvious furcella to milter rhynchodaenm white pearl star, gently
Pressure belly is that the seminal fluid for having white thick flows out；
（5）Injection ocyodinic：
Bar house oil fish milter is injected using a pin, and injection site is thoracic cavity, lucifuge sunshade during injection；Raun ocyodinic selects Malaysia
Sour DOM presses per kilogram of body weight 10-20 μ g and HCG per kilograms by per kilogram of body weight 2-6mg and Luteinizing hormone releasing-hormone A2
1000-1500IU carries out intramuscular injection, and bar house oil fish milter suitably reduces dosage depending on mature condition or do not inject；Milter can also kill
Kill taking-up seminal vesicle and produce spermary liquid, spermary liquid can use Ge Linshi liquid or glucose injection as preserving liquid, and prepare and
The spermatozoa preservative fluid of sperm isoelectric point.At 18~25 DEG C, injection implements artificial aiding-birth after 24 hours for water temperature control during artificial induced spawning.
（6）The management of parent population before spawning
After the injection of parent population ocyodinic, it is put into constructed fishpond, shading simultaneously keeps environment quiet.
（7）Artificial insemination：
24 hours methods with dry method of artificial fertilization carry out artificial insemination after the injection of parent population ocyodinic；Embryonated egg uses unsticking or use
Artificial fish nest collects embryonated egg.
（8）Artificial incubation：
Embryonated egg that artificial insemination is obtained is cleaned, go the removal of impurity, or the mould proof sterilization of unsticking after, be put into hatchery shade net
Sunshade is hatched；Miniflow water, 18-25C ° of hatching coolant-temperature gage holding, more than water body dissolved oxygen amount 5mg/L are kept, and carries out anti-water mold
Sterilization.
（9）Seed rearing
After 3-5 days membranes of fry, the flat trip of fry feeds dedicated openings bait, after 14 days, is progressively transitioned into and feeds special full price and match somebody with somebody
Feed powder is closed, more than 3cm is reached when body is long, instruction is raised artificial mixed feed of mediating and sinks to the bottom a material.
Preferably, the environment in the fishpond can be with oneself simulation manufacture, it is desirable to：Water colour, transparency, water temperature, dissolved oxygen, pH value
Content is approached with Bama County of Guangxi disk sun river.
Preferably, the soft fishnet in fishpond is divided into two pieces of isolated areas, it is ensured that female milter is split again each other in same water body
Open, and shading.
Preferably, parent fish pond will possess flowing water flow in pipes and irrigation and drainage are convenient, manually manufacture current, with stimulation by running water parent
Fish.
Preferably, thimerosal is carried out disinfection using pure Chinese medicinal preparation FUYINJIE washing lotion to fish body.
Preferably, Chinese medicine preparation FUYINJIE washing lotion concentration be 250 milliliter/cubic metre water bodys, temperature control at 18-22 °C,
Water-bath 1 hour, period and uninterrupted oxygenation.
Preferably, the special group's of the sinking to the bottom material bait of kneading is fed daily twice, and the excrement of bottom of pond is at least extracted daily and water is changed
Once, quantity of exchanged water is the 1/3 of water.Stimulation by running water is started 1 hour in 3 hours in interval to stimulation by running water daily.
Preferably, water temperature is down to 10 °C with cooling-water machine, with cold stimulation by running water, it is cold that flowing is started at daily interval for 3 hours
Spun lacing swashs 1 hour, then warms naturally to normal temperature.
Preferably, Articial bait making sinks to the bottom a material, addition dimension life using the eel special compound feed of protein content 43%
Plain E or zinc gluconate and Selenium in Plants protein-enrichmen culture, until the maturity needed for bar house oil fish reaches artificial insemination.Manually
Before insemination, clomiphene is added in feed, female milter is admixed by fish body 5mg/kg and continuously fed 5 days in feed simultaneously.
Preferably, such as milter maturity not enough, seminal fluid cannot be manually extruded, also milter list pond can be individually cultivated, except pressing
Above feed formula and the kneading group of sinking to the bottom material are outer, can be individually added into Chinese medicine preparation wuzi yanzong pills or Shengjing Decoction etc. and promote patrogenesis
Medicine accelerate the ripening milter sperm.Before artificial insemination, clomiphene is added in feed, female milter admixes feeding by fish body 5mg/kg simultaneously
Continuously fed 5 days in material.
Preferably, bar house oil fish milter is injected using a pin, and injection site is thoracic cavity, lucifuge sunshade during injection.
Preferably, raun ocyodinic selection maleic acid DOM presses per kilogram of body weight 2-6mg and luteinizing hormone releasing hormone
A2 carries out intramuscular injection by per kilogram of body weight 10-20 μ g and HCG per kilograms 1000-1500IU, and bar house oil fish milter regards ripe feelings
Condition suitably reduces dosage or does not inject.
Preferably, milter can also slaughter taking-up seminal vesicle and produce spermary liquid, and spermary liquid can use Ge Linshi liquid or glucose
Parenteral solution prepares the spermatozoa preservative fluid with sperm isoelectric point as preservation liquid.
Preferably, water temperature control, at 18~25 DEG C, artificial aiding-birth is implemented after injecting 24 hours during artificial induced spawning.
Preferably, after the injection of parent population ocyodinic, it is put into constructed fishpond, shading simultaneously keeps environment quiet.
Preferably, 24 hours methods with dry method of artificial fertilization carry out artificial insemination after the injection of parent population ocyodinic；Embryonated egg
Using unsticking or manually fish nest collect embryonated egg.
Preferably, the embryonated egg that artificial insemination is obtained is cleaned, go the removal of impurity, or the mould proof sterilization of unsticking after, be put into and incubate
Change pond shade net sunshade to hatch；Holding miniflow water, 18-25C ° of hatching coolant-temperature gage holding, more than water body dissolved oxygen amount 5mg/L, and
Carry out the sterilization of anti-water mold.
Preferably, dedicated openings bait is for yolk or yolk, soya-bean milk mixing or using the commercial protein for being crushed to 400 mesh
The compound of the eel feed powder material of content 43% or more material.
Specific embodiment
Technical scheme is illustrated below by way of specific instantiation.It should be understood that mention one of the present invention or
Multiple method and steps do not repel the step that also there is other method step before and after the combination step or specifically mentioned at these
Other method step can also be inserted between rapid；It should also be understood that these embodiments are merely to illustrate the present invention rather than limitation
The scope of the present invention.
A kind of bar house oil fish artificial propagation specific method：
Example one：
1：Mimic panel sun Ha Noi environment builds fishpond, and bottom of pond lays one layer of pebble, and carries out disappearing for fishpond and pebble
Poisoning bacterium, the soft fishnet in fishpond is divided into two pieces of isolated areas, it is ensured that female milter separates, and hide again each other in same water body
Light.The mountain spring water and underground river with the disk close water of sun quality of river water or cleaning are put into, parent fish pond will possess flowing water ascending pipe
Road and irrigation and drainage are convenient, manually manufacture current, use stimulation by running water parent population.
2：Fish body is carried out disinfection using pure Chinese medicinal preparation FUYINJIE washing lotion, Chinese medicine preparation FUYINJIE washing lotion concentration is 250
Milliliter/cubic meter water body, temperature control is in 18-22 °C, water-bath 1 hour, period simultaneously uninterrupted oxygenation.
3：Health in vigorous health, appearance is selected normally without the 2-3 age bar house oil fish male and female parent populations for abrading and reaching maturity, to disappear
The good bar house oil fish male and female parent population of poison be respectively put into dictyosome segmentation have flowing water inject and the convenient culture pond of irrigation and drainage in, male and female
Parent population is not only in a water body but also separated from one another, and the special group's of the sinking to the bottom material bait of kneading is fed daily twice, and pond is at least extracted daily
The excrement at bottom and change water once, quantity of exchanged water is the 1/3 of water；Water temperature is down to 10 °C with cooling-water machine, with cold stimulation by running water, often
Flowing cool water is started at its interval for 3 hours to stimulate 1 hour, then warms naturally to normal temperature.Articial bait making is contained using albumen
The eel special compound feed of amount 43% sinks to the bottom a material, vitamin E and Selenium in Plants protein-enrichmen culture is added, until bar house oil fish
Maturity needed for reaching artificial insemination.Before artificial insemination, clomiphene is added in feed, female milter presses fish body 5mg/kg simultaneously
Admix and continuously fed 5 days in feed.
4：Annual 4-7 months, physique healthy and strong, disease-free without wound is selected, the good parent population of gonad development carries out artificial induced spawning；Property into
Ripe bar horse becomes yellow and deepens by original yellow green, female, male to be easier to differentiate；Raun belly expands, accidental ovary profile, lets out
Kong Weihong is grown, belly is touched, feel has certain full, pliability；Substantially, hand touches with obvious thorn to milter rhynchodaenm white pearl star
Prominent, light pressure belly is that the seminal fluid for having white thick flows out；
5：Bar house oil fish milter is injected using a pin, and injection site is thoracic cavity, lucifuge sunshade during injection；Raun ocyodinic selects horse
Carry out sour DOM and press per kilogram of body weight:5mg and Luteinizing hormone releasing-hormone A2 press μ g and the HCG per kilograms of per kilogram of body weight 105
1200IU carries out intramuscular injection, and bar house oil fish milter suitably reduces dosage depending on mature condition or do not inject；Water temperature during artificial induced spawning
At 22 DEG C, injection implements artificial aiding-birth after 24 hours for control.
6：After the injection of parent population ocyodinic, it is put into constructed fishpond, shading simultaneously keeps environment quiet.
7：24 hours methods with dry method of artificial fertilization carry out artificial insemination after the injection of parent population ocyodinic；Manually fish nest is received
Collection embryonated egg.
8：Embryonated egg that artificial insemination is obtained is cleaned, go the removal of impurity, after mould proof sterilization, is put into hatchery shade net
Sunshade is hatched；Miniflow water, 22C ° of hatching coolant-temperature gage holding, more than water body dissolved oxygen amount 5mg/L are kept, and carries out disappearing for anti-water mold
Poison.
9:After 3-5 days membranes of fry, the flat trip of fry feeds dedicated openings bait, after 14 days, be progressively transitioned into feed it is special
Perfect compound feed powder, more than 3cm is reached when body is long, and instruction is raised artificial mixed feed of mediating and sinks to the bottom a material.
It is above-described be only presently preferred embodiments of the present invention, it is all made in the range of the spirit and principles in the present invention appoint
What modification, equivalent and improvement etc., should be included within the scope of the present invention.
Claims (10)
1. a kind of bar house oil fish artificial fecundation method, comprises the following steps：
（1）The foundation in fishpond：
Selection builds fishpond in Bama County of Guangxi or periphery mimic panel sun Ha Noi environment, and bottom of pond lays one layer of pebble, and does
Good fishpond and the disinfection of pebble, the soft fishnet in fishpond are divided into two pieces of isolated areas, it is ensured that female milter is in same water
Body, separates, and shading again each other, is finally putting into the mountain spring water and subterranean stream of the water close with disk sun quality of river water or cleaning
Water, parent fish pond will possess flowing water flow in pipes and irrigation and drainage are convenient, manually manufacture current, use stimulation by running water parent population；
(2) fish body sterilization：
Fish body is carried out disinfection using pure Chinese medicinal preparation FUYINJIE washing lotion, Chinese medicine preparation FUYINJIE washing lotion concentration is 250 milliliters/it is vertical
Square meter Shui Ti, temperature control is in 18-22 °C, water-bath 1 hour, period simultaneously uninterrupted oxygenation；
（3）Adult fish hot housing：
Health in vigorous health, appearance is selected normally without the 2-3 age bar house oil fish male and female parent populations for abrading and reaching maturity, to disinfect
The flowing water that has that bar house oil fish male and female parent population is respectively put into dictyosome segmentation is injected with the convenient culture pond of irrigation and drainage, and male and female parent population was both
It is again separated from one another in a water body, the special group's of the sinking to the bottom material bait of kneading is fed daily twice, the excrement of bottom of pond is at least extracted daily
Just and water is changed once, quantity of exchanged water is the 1/3 of water；Water temperature is down to 10 °C with cooling-water machine, with cold stimulation by running water, is spaced daily
Starting within 3 hours flowing cool water stimulates 1 hour, then warms naturally to normal temperature；
Articial bait making sinks to the bottom a material using the eel special compound feed of protein content 43%, adds vitamin E or glucose
Sour zinc and Selenium in Plants protein-enrichmen culture, until the maturity needed for bar house oil fish reaches artificial insemination；
Such as milter maturity not enough, seminal fluid cannot be manually extruded, also milter list pond can be individually cultivated, except by above feed formula
And mediate and sink to the bottom that material is outer, Chinese medicine preparation wuzi yanzong pills or Shengjing Decoction etc. can be individually added into promote the medicine of patrogenesis and accelerate the ripening milter
Sperm, before artificial insemination, adds clomiphene in feed, female milter is admixed by fish body 5mg/kg and continuously feed 5 in feed simultaneously
My god；
（4）The selection of parent population：
Annual 4-7 months, physique healthy and strong, disease-free without wound is selected, the good parent population of gonad development carries out artificial induced spawning；Sexually matured bar
Horse becomes yellow and deepens by original yellow green, female, male to be easier to differentiate；Raun belly expands, and accidental ovary profile, cloacal aperture is micro-
It is red, belly is touched, feel has certain full, pliability；Substantially, hand touches with obvious furcella to milter rhynchodaenm white pearl star, gently
Pressure belly is that the seminal fluid for having white thick flows out；
（5）Injection ocyodinic：
Bar house oil fish milter is injected using a pin, and injection site is thoracic cavity, lucifuge sunshade during injection；Raun ocyodinic selects Malaysia
Sour DOM presses per kilogram of body weight 10-20 μ g and HCG per kilograms by per kilogram of body weight 2-6mg and Luteinizing hormone releasing-hormone A2
1000-1500IU carries out intramuscular injection, and bar house oil fish milter suitably reduces dosage depending on mature condition or do not inject；Milter can also kill
Kill taking-up seminal vesicle and produce spermary liquid, spermary liquid can use Ge Linshi liquid or glucose injection as preserving liquid, and prepare and
The spermatozoa preservative fluid of sperm isoelectric point, at 18~25 DEG C, injection implements artificial aiding-birth after 24 hours for water temperature control during artificial induced spawning；
（6）The management of parent population before spawning：
After the injection of parent population ocyodinic, it is put into constructed fishpond, shading simultaneously keeps environment quiet；
（7）Artificial insemination：
24 hours methods with dry method of artificial fertilization carry out artificial insemination after the injection of parent population ocyodinic；Embryonated egg uses unsticking or use
Artificial fish nest collects embryonated egg；
（8）Artificial incubation：
Embryonated egg that artificial insemination is obtained is cleaned, go the removal of impurity, or the mould proof sterilization of unsticking after, be put into hatchery shade net
Sunshade is hatched；Miniflow water, 18-25C ° of hatching coolant-temperature gage holding, more than water body dissolved oxygen amount 5mg/L are kept, and carries out anti-water mold
Sterilization；
（9）Seed rearing
After 3-5 days membranes of fry, the flat trip of fry feeds dedicated openings bait, after 14 days, is progressively transitioned into and feeds special full price and match somebody with somebody
Feed powder is closed, more than 3cm is reached when body is long, instruction is raised artificial mixed feed of mediating and sinks to the bottom a material.
2. a kind of bar house oil fish artificial fecundation method as claimed in claim 1, it is characterised in that the environment in the fishpond can be with
Oneself simulation manufacture, it is desirable to：Water colour, transparency, water temperature, dissolved oxygen, pH value content and Bama County of Guangxi disk sun river are approached, described
The soft fishnet in fishpond is divided into two pieces of isolated areas, it is ensured that female milter separates again each other in same water body, and shading.
3. a kind of bar house oil fish artificial fecundation method as claimed in claim 1, it is characterised in that thimerosal uses pure Chinese medicine system
Agent FUYINJIE washing lotion carries out disinfection to fish body, and the Chinese medicine preparation FUYINJIE washing lotion concentration is 250 milliliter/cubic metre water bodys, temperature
Degree control is in 18-22 °C, water-bath 1 hour, period simultaneously uninterrupted oxygenation.
4. a kind of bar house oil fish artificial fecundation method as claimed in claim 1, it is characterised in that feed kneading daily special heavy
Bottom artitificial food twice, at least extracts the excrement of bottom of pond and changes water once daily, and quantity of exchanged water is the 1/3 of water, using cooling-water machine
Water temperature is down to 10 °C, with cold stimulation by running water, interval is started flowing cool water for 3 hours and stimulated 1 hour daily, then is warmed naturally to
Normal temperature.
5. a kind of bar house oil fish artificial fecundation method as claimed in claim 1, it is characterised in that Articial bait making uses egg
The eel special compound feed of Bai Hanliang 43% sinks to the bottom a material, addition vitamin E or zinc gluconate and the training of Selenium in Plants protein-enrichmen
Support, until the maturity needed for bar house oil fish reaches artificial insemination, before artificial insemination, adds clomiphene, female milter in feed
Admixed by fish body 5mg/kg simultaneously and continuously fed 5 days in feed.
6. a kind of bar house oil fish artificial fecundation method as claimed in claim 1, it is characterised in that such as milter maturity not enough,
Seminal fluid cannot be manually extruded, also milter list pond can individually be cultivated, be individually added into Chinese medicine preparation wuzi yanzong pills or Shengjing Decoction etc.
The medicine for promoting patrogenesis accelerates the ripening milter sperm, before artificial insemination, clomiphene is added in feed, and female milter presses fish body simultaneously
5mg/kg admixed and continuously fed 5 days in feed.
7. a kind of bar house oil fish artificial fecundation method as claimed in claim 1, it is characterised in that bar house oil fish milter uses
Pin is injected, and injection site is thoracic cavity, lucifuge sunshade during injection, and raun ocyodinic selection maleic acid DOM presses per kilogram of body weight 2-
6mg and Luteinizing hormone releasing-hormone A2 carry out muscle note by per kilogram of body weight 10-20 μ g and HCG per kilograms 1000-1500IU
Penetrate, bar house oil fish milter suitably reduces dosage depending on mature condition or do not inject, milter can slaughter taking-up seminal vesicle and produce spermary liquid, essence
Nest liquid can use Ge Linshi liquid or glucose injection as preservation liquid, and prepare the spermatozoa preservative fluid with sperm isoelectric point,
At 18~25 DEG C, injection implements artificial aiding-birth after 24 hours for water temperature control during artificial induced spawning.
8. a kind of bar house oil fish artificial fecundation method as claimed in claim 1, it is characterised in that after the injection of parent population ocyodinic,
Be put into constructed fishpond, shading simultaneously keeps environment quiet, parent population ocyodinic injection after 24 hours with dry method of artificial fertilization's
Method carries out artificial insemination；Using unsticking or manually, fish nest collects embryonated egg to embryonated egg.
9. a kind of bar house oil fish artificial fecundation method as claimed in claim 1, it is characterised in that by receiving that artificial insemination is obtained
Smart ovum is cleaned, go the removal of impurity, or the mould proof sterilization of unsticking after, be put into hatchery shade net sunshade hatching；Miniflow water is kept, is incubated
Change 18-25C ° of coolant-temperature gage holding, more than water body dissolved oxygen amount 5mg/L, and carry out the sterilization of anti-water mold.
10. a kind of bar house oil fish artificial fecundation method as claimed in claim 1, it is characterised in that dedicated openings bait for
Yolk or yolk, soya-bean milk mixing or use are crushed to the eel feed powder material of commercial protein content 43% of 400 mesh or more material
Compound sinks to the bottom a material.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107318741A (en) * | 2017-07-06 | 2017-11-07 | 覃忠 | Promote the synchronous fishpond of bar house oil fish parent fish male and female gonad development |
| CN108040924A (en) * | 2017-07-06 | 2018-05-18 | 覃忠 | The method that big mastacembelus aculeatus instruction raises man-made feeds |
| CN109362627A (en) * | 2018-09-17 | 2019-02-22 | 水利部交通运输部国家能源局南京水利科学研究院 | Large-scale tank temperature control system and method for parent population oviposition |
| CN109566481A (en) * | 2017-09-29 | 2019-04-05 | 镇江金山湖农业发展有限公司 | A kind of cultural method of big squama Barb |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101112187A (en) * | 2007-08-01 | 2008-01-30 | 中国海洋大学 | Method for breeding crossocheilus bamaensis |
| CN103651220A (en) * | 2013-12-30 | 2014-03-26 | 玉溪市水产工作站（玉溪市水生动物防疫检疫站云南省渔业船舶检验局玉溪检验处） | Barbodes fuxianensis artificial propagation technology |
| CN105075941A (en) * | 2015-08-05 | 2015-11-25 | 珠海市现代农业发展中心 | Cross breeding method for eleotris oxycephala and oxyeleotris marmoratus |
| CN106359192A (en) * | 2016-08-31 | 2017-02-01 | 湖南白泥湖高科水产养殖有限公司 | Artificial cultivation method for culter alburnus |
| CN106489801A (en) * | 2016-12-12 | 2017-03-15 | 福建省淡水水产研究所 | A kind of half pierces thick lip fish artificial seedling rearing's method |
-
2017
- 2017-03-24 CN CN201710180309.7A patent/CN106879509A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101112187A (en) * | 2007-08-01 | 2008-01-30 | 中国海洋大学 | Method for breeding crossocheilus bamaensis |
| CN103651220A (en) * | 2013-12-30 | 2014-03-26 | 玉溪市水产工作站（玉溪市水生动物防疫检疫站云南省渔业船舶检验局玉溪检验处） | Barbodes fuxianensis artificial propagation technology |
| CN105075941A (en) * | 2015-08-05 | 2015-11-25 | 珠海市现代农业发展中心 | Cross breeding method for eleotris oxycephala and oxyeleotris marmoratus |
| CN106359192A (en) * | 2016-08-31 | 2017-02-01 | 湖南白泥湖高科水产养殖有限公司 | Artificial cultivation method for culter alburnus |
| CN106489801A (en) * | 2016-12-12 | 2017-03-15 | 福建省淡水水产研究所 | A kind of half pierces thick lip fish artificial seedling rearing's method |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107318741A (en) * | 2017-07-06 | 2017-11-07 | 覃忠 | Promote the synchronous fishpond of bar house oil fish parent fish male and female gonad development |
| CN108040924A (en) * | 2017-07-06 | 2018-05-18 | 覃忠 | The method that big mastacembelus aculeatus instruction raises man-made feeds |
| CN109566481A (en) * | 2017-09-29 | 2019-04-05 | 镇江金山湖农业发展有限公司 | A kind of cultural method of big squama Barb |
| CN109362627A (en) * | 2018-09-17 | 2019-02-22 | 水利部交通运输部国家能源局南京水利科学研究院 | Large-scale tank temperature control system and method for parent population oviposition |
| CN109362627B (en) * | 2018-09-17 | 2020-08-11 | 水利部交通运输部国家能源局南京水利科学研究院 | Large-scale water tank temperature control system and method for parent fish spawning experiment |
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