CN105075940A - Artificial cross-breeding method of chromileptes altivelis and epinephelus lanceolatus - Google Patents
Artificial cross-breeding method of chromileptes altivelis and epinephelus lanceolatus Download PDFInfo
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- 241001217936 Epinephelus lanceolatus Species 0.000 title claims abstract description 46
- 241000922680 Cromileptes altivelis Species 0.000 title claims abstract description 43
- 238000000034 method Methods 0.000 title claims abstract description 10
- 238000009402 cross-breeding Methods 0.000 title abstract 6
- 241000251468 Actinopterygii Species 0.000 claims abstract description 65
- 210000004681 ovum Anatomy 0.000 claims abstract description 62
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 45
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 45
- 230000001568 sexual effect Effects 0.000 claims abstract description 14
- 210000004907 gland Anatomy 0.000 claims abstract description 10
- 230000009027 insemination Effects 0.000 claims abstract description 8
- 230000000384 rearing effect Effects 0.000 claims abstract description 8
- 230000004720 fertilization Effects 0.000 claims abstract description 4
- 235000019688 fish Nutrition 0.000 claims description 58
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- 238000004458 analytical method Methods 0.000 claims description 37
- 230000012447 hatching Effects 0.000 claims description 37
- 235000013601 eggs Nutrition 0.000 claims description 26
- 210000000582 semen Anatomy 0.000 claims description 24
- 210000001015 abdomen Anatomy 0.000 claims description 15
- 238000009395 breeding Methods 0.000 claims description 15
- 238000009396 hybridization Methods 0.000 claims description 14
- 230000008859 change Effects 0.000 claims description 13
- 239000013535 sea water Substances 0.000 claims description 13
- 238000007689 inspection Methods 0.000 claims description 10
- 241000238366 Cephalopoda Species 0.000 claims description 9
- 241000269821 Scombridae Species 0.000 claims description 9
- 230000037396 body weight Effects 0.000 claims description 9
- 235000020640 mackerel Nutrition 0.000 claims description 9
- 239000003643 water by type Substances 0.000 claims description 7
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims description 6
- 241000932075 Priacanthus hamrur Species 0.000 claims description 6
- 241001439627 Schistostoma Species 0.000 claims description 6
- 201000010099 disease Diseases 0.000 claims description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 6
- 230000000694 effects Effects 0.000 claims description 6
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- 230000003203 everyday effect Effects 0.000 claims description 4
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- 241000082800 Arthritica semen Species 0.000 claims description 3
- 235000009161 Espostoa lanata Nutrition 0.000 claims description 3
- 240000001624 Espostoa lanata Species 0.000 claims description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 3
- 241001062009 Indigofera Species 0.000 claims description 3
- 229930003427 Vitamin E Natural products 0.000 claims description 3
- 239000012467 final product Substances 0.000 claims description 3
- 238000011010 flushing procedure Methods 0.000 claims description 3
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims description 3
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- 238000003756 stirring Methods 0.000 claims description 3
- 239000013589 supplement Substances 0.000 claims description 3
- 229940046009 vitamin E Drugs 0.000 claims description 3
- 235000019165 vitamin E Nutrition 0.000 claims description 3
- 239000011709 vitamin E Substances 0.000 claims description 3
- 238000007599 discharging Methods 0.000 claims description 2
- 235000013372 meat Nutrition 0.000 abstract description 4
- 230000001360 synchronised effect Effects 0.000 abstract 2
- 230000009286 beneficial effect Effects 0.000 abstract 1
- 235000021403 cultural food Nutrition 0.000 abstract 1
- 230000007613 environmental effect Effects 0.000 abstract 1
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- 238000011534 incubation Methods 0.000 abstract 1
- 235000015097 nutrients Nutrition 0.000 abstract 1
- 230000001488 breeding effect Effects 0.000 description 4
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses an artificial cross-breeding method of chromileptes altivelis and epinephelus lanceolatus. The artificial cross breeding method comprises following steps: (1) selection of parents; (2) parent fish rearing of chromileptes altivelis; (3) parent fish rearing of epinephelus lanceolatus; (4) judgments of oestrus and spawning of parent fish; (5) artificial fertilization; and (6) incubation of fertilized ovum. The artificial cross-breeding method of chromileptes altivelis and epinephelus lanceolatus has following beneficial effects: chromileptes altivelis is selected as female parents and epinephelus lanceolatus is utilized as male parents; by selecting different modes, two kinds of parents are reared in order to achieve synchronous maturity of two kinds of parents; by nutrient enrichment and environmental control of parents, synchronized development of two kinds of sexual glands of parent fish is prompted and quality of ova and sperms is improved in order to obtain mature ova and sperms; larva fish is hatched by artificial insemination in order to obtain larva fish in the cross-breeding first filial generation having parent characteristics of two kinds; and reared cross-breeding fish has food culture performance and delicious meat and high cost performance.
Description
Technical field
The present invention relates to technical field of aquaculture, be specifically related to the artificial hybridization breeding method of a kind of analysis of Cromileptes altivelis and epinephelus lanceolatus fish.
Background technology
Analysis of Cromileptes altivelis (formal name used at school: Chromileptesaltivelis), be subordinate to Perciformes, Sushi section, analysis of Cromileptes altivelis belongs to, also known as mouse spot, for tropical dwelling property of seawater reef fish, main area is the Indian Ocean, coastal and Western Pacific, Africa, and South Sea of China, the Taiwan Straits and Hong Kong Waters have distribution.Analysis of Cromileptes altivelis generally inhabits right up and down lagoon region or the better coral reef water of water quality, perches at about 15m depth of water place.Juvenile fish color is beautiful, can be used as pet fish, and delicious flavour after growing up is very senior food fish.
Epinephelus lanceolatus fish (
formal name used at school: Epinepheluslanceolatus), the wholesale of popular name dragon, rough gentian lithosporic is the kind that in ablen, build is maximum, large with build, growth is famous soon.Epinephelus lanceolatus is a kind of food fish with high economic worth, propagates artificially now in south China province, Taiwan, Australia, Hong Kong and Southeast Asian countries and regions
Analysis of Cromileptes altivelis delicious meat, price is higher on the market, but growth is slow; Epinephelus lanceolatus fish have individual large, grow fast advantage, be a kind of food fish with high economic worth, and meat is better.
For obtaining the hybridization first filial generation having two kinds of parent's features concurrently, provide a kind of method of analysis of Cromileptes altivelis and epinephelus lanceolatus fish artificial hybridization breeding.
Summary of the invention
Problem to be solved by this invention is, for the deficiency of natural species in economic characters, designs a kind of intergeneric cross method of grouper, can obtain the batch prelarva having two parent's advantages concurrently, produce for large-scale cultivation.
The technical scheme that the present invention adopts for its problem of solution is:
An artificial hybridization breeding method for analysis of Cromileptes altivelis and epinephelus lanceolatus fish, comprises the following steps:
(1) Juvenile stage
A. female parent: the healthy analysis of Cromileptes altivelis selecting 5 ages more than, build normal, disease-free, energetic is as female parent;
B. male parent: the healthy epinephelus lanceolatus fish selecting 6 ages more than, build normal, disease-free, energetic is as male parent;
(2) raising in cage of analysis of Cromileptes altivelis parent population
A. sea area is selected and raising in cage: selection year, minimum water temperature was not less than 18 DEG C, seawater salinity 28-33, current 0.2-0.4m/s, and the depth of water is not less than the raising in cage of sea area for parent population of 8m;
B. kind and feeding volume is raised: little squid, blue circle Scad, mackerel, throw something and feed every afternoon 1 time, feeding volume controls the 2%-3% in fish body weight, actual feeding volume affects and speed change by the condition such as weather, water temperature, during reinforced cultivating, throw something and feed every day 2 times, each 1 time of morning and afternoon, day feeding volume be the 3-4% of parent population body weight;
(3) pond rearing of epinephelus lanceolatus fish parent fish
A. cultivate pond to cultivate: epinephelus lanceolatus fish selects pond rearing, cultivates pond specification 1 mu, depth of water 3-3.5m, to arrange into discharge outlet each 1;
B. feed kind and feeding volume: mackerel, within three days, throw something and feed 1 time, feeding volume controls the 2%-4% in fish body weight, and actual feeding volume affects and speed change by the condition such as weather, water temperature.
(4) the parent population judgement of oestrusing, laying eggs:
A. sea area water temperature rises to 24 DEG C, and analysis of Cromileptes altivelis parent population sexual gland starts maturation, selects some individuals once in a week and carry out maturity inspection from parent fish population; From colony, select female analysis of Cromileptes altivelis, be placed on platform and implement to check, in appearance belly protuberance, gonopore is micro-red, gets final product preliminary judgement for reaching sexual maturity;
B. pond water temperature 25-26 DEG C, epinephelus lanceolatus piscinity gland starts maturation, selects some individuals once in a week and carry out maturity inspection from parent fish population; From colony, select male, caught, be placed on suitable platform, cover the eyes of fish with towel, implement to check, gonopore is micro-red, gently presses belly, can extrude milky seminal fluid, namely reaches ripe;
(5) artificial insemination
A. semen collection: find in sexual gland inspection that the epinephelus lanceolatus fish that seminal fluid is good is individual, i.e. desirable essence, with dry towel, fish belly portion is dried, cotton ball soaked in alcohol is sterilized to gonopore, with hand at fish body belly, push from forward direction gonopore direction, appoint milky seminal fluid to flow out from gonopore, use 50ml syringe, seminal fluid is drawn;
B. ovum is squeezed: be placed on platform by female for analysis of Cromileptes altivelis parent population, with wet towel, fish body is wrapped up, expose gonopore, operator puts on emgloves, insert the gonopore of fish with finger, gonopore is opened, pushes from forward direction gonopore direction in fish belly portion with hand, mature egg can flow out, and catches the ovum of outflow below gonopore with basin;
C. artificial insemination
Clean seawater is added in the basin filling ovum, add water while stir gently, with entering to add epinephelus lanceolatus milt liquid for subsequent use, seminal fluid addition is per kilogram ovum 1ml seminal fluid, after leaving standstill 3-4min after mixing, with clean seawater flushing fertilized egg 3-4 time;
(6) hatching of fertilized egg
Prepare a volume 500L, hatching pail that bottom is provided with draining valve, the inflation of gas stone is placed in hatching pail, the fertilized egg processed through step (5) is put into hatching pail hatch, incubating oosperm condition is water temperature 25-30 DEG C, salinity 28-33, pH7.8 ~ 8.5, hatching density is 250-300 ten thousand fertilized egg/m
3, find wherein have when dead ovum or unfertilized egg and will get rid of in time, after dead ovum or unfertilized egg are drained, within every 2 hours, change water once, each quantity of exchanged water is the half of hatching pail volume, to keep the pure and fresh of hatching pail maritime interior waters.
In the present invention, in the b of described step (2), according to the size determination feed block size of parent population schistostoma, the feed fish block that analysis of Cromileptes altivelis parent population is applicable to, long 5-8cm, wide 2-3cm, little squid can be thrown something and fed by whole piece; Individual larger squid is cut into two sections and throws something and feeds, and onesize indigo plant circle Scad, decaptitating truncates throws something and feeds.
In the present invention, in the b of described step (3), according to the size determination feed block size of parent population schistostoma, the feed fish block that epinephelus lanceolatus fish parent fish is applicable to, long 10-15cm, wide 5-8cm, generally, mackerel need be excised end to end, and whole piece is thrown something and fed; During reinforced cultivating, in every block feed, fill in vitamin E soft capsule 1 (50mg) throw something and feed.
In the present invention, in a of described step (5), the seminal fluid taken out is injected vial, sealing, keep in for 4 DEG C, for subsequent use, temporary seminal fluid still had fertilizing ability in one week.
In the present invention, in the b of described step (5), cultivate the bow-backed fish parent fish in net cage, after reaching sexual maturity, can natural spawning, now must conscientiously observe group activity situation, find that parent population does not ingest, then may lay eggs the same day, must prepare at any time manually to get ovum, once find that there is parent population have mating behavior, namely immediately raun is caught, implemented to squeeze ovum.
In the present invention, in described step (6), get rid of dead ovum or be not subject to the method for ovum: allowing gas stone stop inflation 10 ~ 15min, normal fertilized egg can be floated after stopping the supple of gas or steam, and dead ovum or unfertilized egg and a small amount of foreign material will be sunken to the bottom of hatching pail, open the draining valve bottom hatching pail, slow draining, checkmate ovum or unfertilized egg and foreign material are discharged, draining is on one side while check, when there is normal fertilization ovum in the water of discharging, then close draining valve, stop draining, recover inflation, and supplement seawater to normal water level to hatching pail, after dead ovum or unfertilized egg are drained, within every 2 hours, change water once, each quantity of exchanged water is the half of hatching pail volume, to keep the pure and fresh of hatching pail maritime interior waters.
In sum, the present invention is a kind of Epinephelus intermolecular hybrid breeding method, the present invention selects analysis of Cromileptes altivelis as female parent, epinephelus lanceolatus fish as male parent, by selecting different modes to cultivate two kinds of parents, reaching two kinds of parents synchronously ripe, and fortification, environment conditioning are carried out to parent, to promote two kinds of fish parent fish gonad developments synchronously and to improve ovum and sperm quality, obtain ripe essence, ovum, then carry out artificial insemination, hatch prelarva.Present invention achieves the intergeneric cross of analysis of Cromileptes altivelis and epinephelus lanceolatus fish, obtain the prelarva of the hybridization first filial generation fish having two kinds of parent's features concurrently, propagate artificially for follow-up, the hybridization fish culture performance cultivated is good, and delicious meat, cost performance is high.
Embodiment
Clear, complete description is carried out, to understand object of the present invention, characteristic sum effect fully below with reference to the technique effect of embodiment to design of the present invention and generation.Obviously; described embodiment is a part of embodiment of the present invention, instead of whole embodiment, based on embodiments of the invention; other embodiments that those skilled in the art obtains under the prerequisite not paying creative work, all belong to the scope of protection of the invention.
An artificial hybridization breeding method for analysis of Cromileptes altivelis and epinephelus lanceolatus fish, comprises the following steps:
(1) Juvenile stage
A. female parent: the healthy analysis of Cromileptes altivelis selecting 5 ages more than, build normal, disease-free, energetic is as female parent;
B. male parent: the healthy epinephelus lanceolatus fish selecting 6 ages more than, body build normal, disease-free, energetic is as male parent;
For ensureing that two kinds of parent populations synchronously reach sexual maturity.Epinephelus lanceolatus fish selects indoor water mud sump to heat cultivations, analysis of Cromileptes altivelis selection sea area raising in cage.
(2) raising in cage of analysis of Cromileptes altivelis parent population
A. sea area is selected: selection year, minimum water temperature was not less than 18 DEG C, seawater salinity 28-33, current 0.2-0.4m/s, the depth of water is not less than the raising in cage of sea area for parent population of 8m, cultivation cage size is 6m × 6m × 5m, mesh 2a=5cm, cultivates density 300-500 tail/case.In cultivating process, often need change etting, prevent mesh blocked, the water body affecting net cage exchanges;
B. kind and feeding volume is raised: little squid, blue circle Scad, mackerel, throw something and feed every afternoon 1 time, feeding volume controls the 2%-3% in fish body weight, actual feeding volume increases and decreases by the condition such as weather, water temperature affects, during reinforced cultivating, throw something and feed every day 2 times, each 1 time of morning and afternoon, day feeding volume be the 3%-4% of parent population body weight;
(3) pond rearing of epinephelus lanceolatus fish parent fish
A. cultivate pond: epinephelus lanceolatus fish selects pond rearing, cultivate density 50-60 tail/mu; Pond water source clean, cultivates pool area 1 mu, depth of water 3-3.5m, to arrange into discharge outlet each 1; For keeping water source to clean, every day need change water 10-15%; Winter adds a cover polyethylene agricultural film above pond, plays the effect of insulation;
B. feed kind and feeding volume: mackerel, within three days, throw something and feed 1 time, feeding volume controls the 2%-4% in fish body weight, and actual feeding volume affects and speed change by the condition such as weather, water temperature;
(4) the parent population judgement of oestrusing, laying eggs:
The gonad development of parent population is by water temperature regulate and control.Water temperature reaches 25 DEG C, and analysis of Cromileptes altivelis enters breeding period.Water temperature reaches 27 DEG C, and epinephelus lanceolatus fish enters breeding period.For sea area, Sanya, in the middle ten days in March, sea area water temperature rises to 23 DEG C; The epinephelus lanceolatus fish of being now stamped warm canopy cultivates pond, about 25 DEG C of water temperature.Reinforced cultivating can be carried out to two kinds of parent populations simultaneously.
A. sea area water temperature rises to 24 DEG C, analysis of Cromileptes altivelis parent population sexual gland starts maturation, from parent fish population, select some individuals carries out maturity inspection once in a week, female analysis of Cromileptes altivelis is selected from colony, be placed on platform, implement to check, in appearance belly protuberance, gonopore is micro-red, gets final product preliminary judgement for reaching sexual maturity;
B. pond water temperature 25-26 DEG C, epinephelus lanceolatus piscinity gland starts maturation, selects some individuals once in a week and carry out maturity inspection from parent fish population; From colony, select male, caught, be placed on suitable platform, the eyes of lid fish gathering with towel, implement to check, gonopore is micro-red, gently presses belly, can extrude milky seminal fluid, namely reaches ripe;
(5) artificial insemination
A. semen collection: individual to the epinephelus lanceolatus fish extruding seminal fluid, find that seminal fluid is good in sexual gland inspection, i.e. desirable essence, dried in fish belly portion with dry towel, cotton ball soaked in alcohol is sterilized to gonopore, with hand at fish body belly, push from forward direction gonopore direction, appoint milky seminal fluid to flow out from gonopore, use 50ml syringe, seminal fluid is drawn;
B. squeeze ovum: be placed on platform by female for analysis of Cromileptes altivelis parent population, with wet towel, fish body is wrapped up, expose gonopore, operator puts on emgloves, inserts the gonopore of fish, gonopore is opened with finger, push from forward direction gonopore in fish belly portion with hand, mature egg can flow out.Catch the ovum of outflow with dish below gonopore;
C. artificial insemination
In the basin filling ovum, add clean seawater, add water and stir gently, with entering to add epinephelus lanceolatus milt liquid for subsequent use, seminal fluid addition is per kilogram ovum 1ml, leaves standstill 3-4min, with clean seawater flushing fertilized egg after mixing;
(6) hatching of fertilized egg
Prepare a volume 500L, hatching pail that bottom is provided with draining valve, the inflation of gas stone is placed in hatching pail, the fertilized egg processed through step (5) is put into hatching pail hatch, incubating oosperm condition is water temperature 25-30 DEG C, salinity 28-33, pH7.8 ~ 8.5, hatching density is 250-300 ten thousand fertilized egg/m
3, find wherein have when dead ovum or unfertilized egg and will get rid of in time, after dead ovum or unfertilized egg are drained, within every 2 hours, change water once, each quantity of exchanged water is the half of hatching pail volume, to keep the pure and fresh of hatching pail maritime interior waters.
Further, in the b of described step (2), according to the size determination feed block size of parent population schistostoma, the feed fish block length 5-8cm that analysis of Cromileptes altivelis parent population is applicable to, wide 2-3cm, little squid can be thrown something and fed by whole piece; Individual larger squid is cut into two sections and throws something and feeds, and onesize indigo plant circle Scad, decaptitating truncates throws something and feeds.
Further, in the b of described step (3), according to the size determination feed block size of parent population schistostoma, the feed fish block that epinephelus lanceolatus fish parent fish is applicable to, long 10-15cm, wide 5-8cm, generally, mackerel need be excised end to end, and whole piece is thrown something and fed; During reinforced cultivating, in every block feed, fill in vitamin E soft capsule 1 (50mg) throw something and feed.
Further, in a of described step (5), by the seminal fluid injecting tube taken out, sealing, keeps in for 4 DEG C, and for subsequent use, temporary seminal fluid still had fertility in one week.
Further, in the b of described step (5), cultivate the bow-backed fish parent fish in net cage, after reaching sexual maturity, can natural spawning, now must conscientiously observe group activity situation, find that parent population does not ingest, then may lay eggs the same day, must prepare at any time manually to get ovum, once find that there is parent population have mating behavior, namely immediately raun is caught, implemented to squeeze ovum.
Further, in described step (6), get rid of dead ovum or be not subject to the method for ovum: allowing gas stone stop inflation 10 ~ 15min, normal fertilized egg can be floated after stopping the supple of gas or steam, and dead ovum or unfertilized egg and a small amount of foreign material will be sunken to the bottom of hatching pail, open the draining valve bottom hatching pail, slow draining, checkmate ovum or unfertilized egg and foreign material are discharged, and draining is inspection on one side, when there is normal fertilization ovum in the water of discharge, then close draining valve, stop draining, recover inflation, and supplement seawater to normal water level to hatching pail.After dead ovum or unfertilized egg are drained, within every 2 hours, change water once, each quantity of exchanged water is the half of hatching pail volume, to keep the pure and fresh of hatching pail maritime interior waters.
Finally, hatch 8 hours, after embryonic development to gastrul stage, fertilized egg can be packed, be transported to nursery.Fertilized egg, after hatching 24-26 hour, can hatch prelarva, for artificial breeding.
Above better embodiment of the present invention is illustrated, but the present invention is not limited to described embodiment, those of ordinary skill in the art also can make all equivalent modifications or replacement under the prerequisite without prejudice to spirit of the present invention, and these equivalent modification or replacement are all included in the application's claim limited range.
Claims (6)
1. an artificial hybridization breeding method for analysis of Cromileptes altivelis and epinephelus lanceolatus fish, is characterized in that, comprise the following steps:
(1) Juvenile stage
A. female parent: the healthy analysis of Cromileptes altivelis selecting 5 ages more than, build normal, disease-free, energetic is as female parent;
B. male parent: the healthy epinephelus lanceolatus fish selecting 6 ages more than, build normal, disease-free, energetic is as male parent;
(2) raising in cage of analysis of Cromileptes altivelis parent population
A. sea area is selected and raising in cage: selection year, minimum water temperature was not less than 18 DEG C, seawater salinity 28-33, current 0.2-0.4m/s, and the depth of water is not less than the raising in cage of sea area for parent population of 8m;
B. kind and feeding volume is raised: little squid, blue circle Scad, mackerel, throw something and feed every afternoon 1 time, feeding volume controls the 2%-3% in fish body weight, actual feeding volume affects and speed change by the condition such as weather, water temperature, during reinforced cultivating, throw something and feed every day 2 times, each 1 time of morning and afternoon, day feeding volume be the 3-4% of parent population body weight;
(3) pond rearing of epinephelus lanceolatus fish parent fish
A. cultivate pond to cultivate: epinephelus lanceolatus fish selects pond rearing, cultivates pond specification 1 mu, depth of water 3-3.5m, to arrange into discharge outlet each 1;
B. feed kind and feeding volume: mackerel, within three days, throw something and feed 1 time, feeding volume controls the 2%-4% in fish body weight, and actual feeding volume affects and speed change by the condition such as weather, water temperature.
(4) the parent population judgement of oestrusing, laying eggs:
A. sea area water temperature rises to 24 DEG C, and analysis of Cromileptes altivelis parent population sexual gland starts maturation, selects some individuals once in a week and carry out maturity inspection from parent fish population; From colony, select female analysis of Cromileptes altivelis, be placed on platform and implement to check, in appearance belly protuberance, gonopore is micro-red, gets final product preliminary judgement for reaching sexual maturity;
B. pond water temperature 25-26 DEG C, epinephelus lanceolatus piscinity gland starts maturation, selects some individuals once in a week and carry out maturity inspection from parent fish population; From colony, select male, caught, be placed on suitable platform, cover the eyes of fish with towel, implement to check, gonopore is micro-red, gently presses belly, can extrude milky seminal fluid, namely reaches ripe;
(5) artificial insemination
A. semen collection: find in sexual gland inspection that the epinephelus lanceolatus fish that seminal fluid is good is individual, i.e. desirable essence, with dry towel, fish belly portion is dried, cotton ball soaked in alcohol is sterilized to gonopore, with hand at fish body belly, push from forward direction gonopore direction, appoint milky seminal fluid to flow out from gonopore, use 50ml syringe, seminal fluid is drawn;
B. ovum is squeezed: be placed on platform by female for analysis of Cromileptes altivelis parent population, with wet towel, fish body is wrapped up, expose gonopore, operator puts on emgloves, insert the gonopore of fish with finger, gonopore is opened, pushes from forward direction gonopore direction in fish belly portion with hand, mature egg can flow out, and catches the ovum of outflow below gonopore with basin;
C. artificial insemination
Clean seawater is added in the basin filling ovum, add water while stir gently, with entering to add epinephelus lanceolatus milt liquid for subsequent use, seminal fluid addition is per kilogram ovum 1ml seminal fluid, after leaving standstill 3-4min after mixing, with clean seawater flushing fertilized egg 3-4 time;
(6) hatching of fertilized egg
Prepare a volume 500L, hatching pail that bottom is provided with draining valve, the inflation of gas stone is placed in hatching pail, the fertilized egg processed through step (5) is put into hatching pail hatch, incubating oosperm condition is water temperature 25-30 DEG C, salinity 28-33, pH7.8 ~ 8.5, hatching density is 250-300 ten thousand fertilized egg/m
3, find wherein have when dead ovum or unfertilized egg and will get rid of in time, after dead ovum or unfertilized egg are drained, within every 2 hours, change water once, each quantity of exchanged water is the half of hatching pail volume, to keep the pure and fresh of hatching pail maritime interior waters.
2. according to the artificial hybridization breeding method of analysis of Cromileptes altivelis a kind of in claim 1 and epinephelus lanceolatus fish, it is characterized in that: in the b of described step (2), according to the size determination feed block size of parent population schistostoma, the feed fish block that analysis of Cromileptes altivelis parent population is applicable to, long 5-8cm, wide 2-3cm, little squid can be thrown something and fed by whole piece; Individual larger squid is cut into two sections and throws something and feeds, and onesize indigo plant circle Scad, decaptitating truncates throws something and feeds.
3. according to the artificial hybridization breeding method of analysis of Cromileptes altivelis a kind of in claim 2 and epinephelus lanceolatus fish, it is characterized in that: in the b of described step (3), according to the size determination feed block size of parent population schistostoma, the feed fish block that epinephelus lanceolatus fish parent fish is applicable to, long 10-15cm, wide 5-8cm, generally, mackerel need be excised end to end, and whole piece is thrown something and fed; During reinforced cultivating, in every block feed, fill in vitamin E soft capsule 1 (50mg) throw something and feed.
4. the artificial hybridization breeding method of a kind of analysis of Cromileptes altivelis according to claim 3 and epinephelus lanceolatus fish, is characterized in that: in a of described step (5), and the seminal fluid taken out is injected vial, sealing, keep in for 4 DEG C, for subsequent use, temporary seminal fluid still had fertilizing ability in one week.
5. the artificial hybridization breeding method of a kind of analysis of Cromileptes altivelis according to claim 4 and epinephelus lanceolatus fish, it is characterized in that: in the b of described step (5), cultivate the bow-backed fish parent fish in net cage, after reaching sexual maturity, can natural spawning, now must conscientiously observe group activity situation, find that parent population does not ingest, then may lay eggs the same day, must prepare at any time manually to get ovum, once find that there is parent population have mating behavior, namely immediately raun is caught, implemented to squeeze ovum.
6. the artificial hybridization breeding method of a kind of analysis of Cromileptes altivelis according to claim 5 and epinephelus lanceolatus fish, it is characterized in that: in described step (6), get rid of dead ovum or be not subject to the method for ovum: allowing gas stone stop inflation 10 ~ 15min, normal fertilized egg can be floated after stopping the supple of gas or steam, and dead ovum or unfertilized egg and a small amount of foreign material will be sunken to the bottom of hatching pail, open the draining valve bottom hatching pail, slow draining, checkmate ovum or unfertilized egg and foreign material are discharged, draining is on one side while check, when there is normal fertilization ovum in the water of discharging, then close draining valve, stop draining, recover inflation, and supplement seawater to normal water level to hatching pail.After dead ovum or unfertilized egg are drained, within every 2 hours, change water once, each quantity of exchanged water is the half of hatching pail volume, to keep the pure and fresh of hatching pail maritime interior waters.
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Denomination of invention: Artificial cross-breeding method of chromileptes altivelis and epinephelus lanceolatus Effective date of registration: 20200616 Granted publication date: 20180417 Pledgee: Agricultural Development Bank of China Lingshui Li Autonomous County sub branch Pledgor: HAINAN CHENHAI AQUATIC Co.,Ltd. Registration number: Y2020980003102 |
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