CN101940175A - Method for breeding 'blue spot' hybrids - Google Patents
Method for breeding 'blue spot' hybrids Download PDFInfo
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- CN101940175A CN101940175A CN201010249407XA CN201010249407A CN101940175A CN 101940175 A CN101940175 A CN 101940175A CN 201010249407X A CN201010249407X A CN 201010249407XA CN 201010249407 A CN201010249407 A CN 201010249407A CN 101940175 A CN101940175 A CN 101940175A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses a method for breeding 'blue spot' hybrids. In the invention, hybrid 'blue spot' fry is produced by selecting single female epinephelus coioides as a female parent and single male epinephelus lanceolatus as a male parent and by distant hybridization, artificial parturition expediting, insemination, incubation and fry breeding. The method for breeding the 'blue spot' hybrids of the invention is simple in operation and suitable for mass production; the bred 'blue spot' fry has a survival rate of 5 to 10 percent and a breeding survival rate of over 95 percent, and both the survival rates are higher than those of the common groupers, so that the bred 'blue spot' fry has a promising market prospect.
Description
Technical field
The present invention relates to a kind of seawater fish breeding method, be specifically related to the breeding method of a kind of hybridization " Green Dragon spot " fry.
Background technology
Grouper (Epinephelus sp.) is one of famous and precious seafood fish renowned in the world, its fine and tender taste, delicious flavour, and market price height, in demand.FAO (Food and Agriculture Organization of the United Nation) classified grouper, tuna and yellow tail Yellowtail as following three kinds of the most rising cultured fishes in 20 years in 2004.In recent years, China, Taiwan, Japan, Korea S, Southeast Asia and Oceanian many countries are all actively developing propagating artificially of grouper.
Epinephelus coioides (Epinephelus coioides) individual median size in Epinephelus, growth rate is general, and parent fish rearing and seedling breeding are easier to relatively, and technology is ripe at present, obtains mature egg or fertilized egg easily by hastening parturition.Epinephelus lanceolatus fish (Epinephelus lanceolatus) then is individual maximum fish in the Epinephelus, growth rate is ultrafast, it is ripe that parent fish rearing at present and seedling breeding technology are still owed, and obtaining of mature egg or fertilized egg is quite difficult, but it is then relatively easy to obtain seminal fluid from ripe milter.
Crossbreeding is the most classical breeding method.Dissimilar parents are hybridized, and can obtain reconfiguring and filial generation that type is abundant of gene.Some type may be the combination of parents' merit, super parents' merit also may occur.Certainly, the combination of parents' inferior position proterties in filial generation, also may occur, even super parents' inferior position proterties might occur.Therefore, the correctness that hybrid strain is selected is the heterosis utilization key of success.Both at home and abroad about the more existing researchs of seawater fish crossbreeding, but the seawater fish of breeding improved seeds by hybridization also seldom.
Summary of the invention
The object of the present invention is to provide the breeding method of a kind of hybridization " Green Dragon spot " fry, improved seeds are bred in this method hybridization, and are simple and practical, are easy to large-scale production.
The object of the invention can realize by following technical measures: the breeding method of a kind of hybridization " Green Dragon spot " fry, and it is maternal selecting Epinephelus coioides raun individuality; Selecting epinephelus lanceolatus fish milter individuality is male parent; Carry out distant hybridization, cultivate by artificial induced spawning, insemination, hatching, young postlarva and produce hybridization " Green Dragon spot " fry.
Maternal Epinephelus coioides raun of the present invention requires belly obviously to expand, and the development of ovary is to the IV phase; Described male parent epinephelus lanceolatus fish milter requires to push belly adularescent seminal fluid and flows out.
Only maternal Epinephelus coioides raun is hastened parturition in the process of hastening parturition of the present invention, male parent epinephelus lanceolatus fish milter is not implemented to hasten parturition.
The present invention compares with existing lithosporic fry rearing technology, has following advantage:
1, distant hybridization combination fertilization rate and the incubation rate height selected of the present invention, fertilization rate can reach more than 98%, and incubation rate can reach more than 80%;
2, " Green Dragon spot " fry of utilization the inventive method cultivation has the obvious growth advantage, fast 3 times than parent Epinephelus coioides of " Green Dragon spot " fry current growth speed;
3, " Green Dragon spot " survival rate of fish fry height of utilization the inventive method cultivation, " Green Dragon spot " survival rate of seedling are cultured survival rate more than 95% at 5-10%, all are significantly higher than common grouper;
4, " Green Dragon spot " of the present invention breeding method is simple to operate, parental line selection, hasten parturition, artificial insemination, method of operating such as hatch, grow seedlings more easily grasps, and is fit to large-scale production;
5, " Green Dragon spot " fry of producing of the inventive method not only grows soon, and adaptability is strong, premunition is strong, the bodily form is attractive in appearance, Fresh ﹠ Tender in Texture, good market prospects.
Description of drawings
Fig. 1 is the present invention's hybridization " Green Dragon spot " and parents' profile schematic diagram.
Embodiment
Hybridization " Green Dragon spot " culture experiment concrete grammar step is as follows:
(1) parent fish rearing and screening: parent population is raised at ordinary times in the parent fish rearing pond, the new iced trash fish of throwing something and feeding, beginning preceding 1 month of mating season reinforced cultivating, the parent population fortified feed of throwing something and feeding.Preceding parent population is risen of breeding caught, and carries out proterties and sexual gland inspection one by one.Select parent population healthy and strong, that body surface does not have wound, nothing deformity; The Epinephelus coioides raun requires belly obviously to expand, and the development of ovary is to the IV phase; Epinephelus lanceolatus fish milter requires the light belly adularescent seminal fluid of pressing to flow out;
(2) hybrid strain apolegamy: select for use the Epinephelus coioides raun individual for maternal during breeding, epinephelus lanceolatus fish milter individuality is a male parent;
(3) hasten parturition: in grouper mating season, to the maternal injection of the Epinephelus coioides raun oxytocic hormone that chooses, carry out the routine process of hastening parturition, oxytocic drug is luteotropin releasing hormone d-ala analog LHRH-A2 and human chorionic gonadtropin HCG hybrid injection; Epinephelus lanceolatus fish milter male parent is not implemented to hasten parturition.
(4) artificial insemination: the about 42h in back that hastens parturition adopts conventional dry method of artificial fertilization's operation, obtains the exomixis ovum.
(5) hatching: fertilized egg places carries out conventional net cage hatching under net cage flowing water, the venting condition.
(6) fry rearing: the prelarva after will hatching is at last rendered in the sea pond, carries out routine with pond ecosystem seedling raising process and cultivates.
By above-mentioned implementation process, in August, 2009 hybridization " Green Dragon spot " culture experiment, obtain the exomixis ovum, fertilization rate is up to 99.5%; Hatchability of fertile eggs reaches 82%; Cultivate " Green Dragon spot " fry 43800 tails of 3cm specification; " Green Dragon spot " fry was raised three months, and growth rate is than parent Epinephelus coioides fast 303%.
By above-mentioned implementation process, in September, 2009 hybridization " Green Dragon spot " culture experiment, obtain the exomixis ovum, fertilization rate is up to 98.5%; Hatchability of fertile eggs reaches 80%; Cultivate " Green Dragon spot " fry 76500 tails of 3cm specification; " Green Dragon spot " fry was raised three months, and growth rate is than parent Epinephelus coioides fast 295%.
The present invention can cultivate the first filial generation with obvious growth vigor---Green Dragon spot, and simple and practical, be easy to large-scale production.
Claims (3)
1. the breeding method of a hybridization " Green Dragon spot " is characterized in that: select the Epinephelus coioides raun individual for maternal; Selecting epinephelus lanceolatus fish milter individuality is male parent; Carry out distant hybridization, after artificial induced spawning, insemination, hatching, cultivation, produce hybridization " Green Dragon spot " fry.
2. the method for cultivation according to claim 1 " Green Dragon spot " fry is characterized in that: described maternal Epinephelus coioides raun requires abdominal distention, and the development of ovary is to the IV phase; Described male parent epinephelus lanceolatus fish milter requires to push belly adularescent seminal fluid and flows out.
3. the method for cultivation according to claim 1 " Green Dragon spot " fry is characterized in that: only maternal Epinephelus coioides raun is hastened parturition in the described process of hastening parturition, male parent epinephelus lanceolatus fish milter is not implemented to hasten parturition.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201010249407.XA CN101940175B (en) | 2010-08-09 | 2010-08-09 | Method for breeding 'blue spot' hybrids |
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| CN201010249407.XA CN101940175B (en) | 2010-08-09 | 2010-08-09 | Method for breeding 'blue spot' hybrids |
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| CN101940175A true CN101940175A (en) | 2011-01-12 |
| CN101940175B CN101940175B (en) | 2014-05-07 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105075940A (en) * | 2015-07-14 | 2015-11-25 | 海南晨海水产有限公司 | Artificial cross-breeding method of chromileptes altivelis and epinephelus lanceolatus |
| CN107027661A (en) * | 2015-12-24 | 2017-08-11 | 韩国海洋科学技术院 | Cenospecies and its production method of the brown grouper with epinephelus lanceolatus fish |
| CN113711955A (en) * | 2021-06-30 | 2021-11-30 | 广东省海洋渔业试验中心 | Method for improving green-red hybrid spot germplasm |
-
2010
- 2010-08-09 CN CN201010249407.XA patent/CN101940175B/en active Active
Non-Patent Citations (4)
| Title |
|---|
| 《中山大学学报(自然科学版)》 20070531 刘付永忠等 赤点石斑鱼♂与斜带石斑鱼♀杂交的初步研究 第46卷, 第03期 2 * |
| 《南方水产》 20080430 区又君等 5种石斑鱼遗传差异的RAPD分析 56－62 第4卷, 第02期 2 * |
| 《海洋通报》 20080630 符书源等 海南近海野生鞍带石斑鱼群体遗传多样性的RAPD分析 25－31 第27卷, 第03期 2 * |
| 《遗传》 20070731 董秋芬等 9种石斑鱼遗传多样性和系统发生关系的微卫星分析 837－843 第29卷, 第07期 2 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105075940A (en) * | 2015-07-14 | 2015-11-25 | 海南晨海水产有限公司 | Artificial cross-breeding method of chromileptes altivelis and epinephelus lanceolatus |
| CN107027661A (en) * | 2015-12-24 | 2017-08-11 | 韩国海洋科学技术院 | Cenospecies and its production method of the brown grouper with epinephelus lanceolatus fish |
| TWI629362B (en) * | 2015-12-24 | 2018-07-11 | 韓國海洋科學技術院 | An identification method of a hybrid between longtooth grouper epinephelus bruneus and giant grouper epinephelus lanceolatus |
| CN107027661B (en) * | 2015-12-24 | 2020-06-12 | 韩国海洋科学技术院 | Hybrid of Epinephelus fuscoguttatus and Epinephelus lanceolatus and production method thereof |
| CN113711955A (en) * | 2021-06-30 | 2021-11-30 | 广东省海洋渔业试验中心 | Method for improving green-red hybrid spot germplasm |
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| CN101940175B (en) | 2014-05-07 |
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Inventor after: Zhang Haifa Inventor after: Huang Peiwei Inventor after: Zhang Lei Inventor after: Lin Haoran Inventor after: Zhang Yong Inventor after: Wang Yunxin Inventor after: Li Shuisheng Inventor after: Huang Guoguang Inventor after: Liang Weifeng Inventor after: Ou Chonghui Inventor after: Yang Shaosen Inventor before: Zhang Haifa Inventor before: Wang Yunxin Inventor before: Huang Guoguang Inventor before: Liang Weifeng Inventor before: Ou Chonghui Inventor before: Yang Shaosen Inventor before: Huang Peiwei Inventor before: Zhang Lei |
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Free format text: CORRECT: INVENTOR; FROM: ZHANG HAIFA WANG YUNXIN HUANG GUOGUANG LIANG WEIFENG OU CHONGHUI YANG SHAOSEN HUANG PEIWEI ZHANG LEI TO: ZHANG HAIFA LIN HAORAN ZHANG YONG WANG YUNXIN LI SHUISHENG HUANG GUOGUANG LIANG WEIFENG OU CHONGHUI YANG SHAOSEN HUANG PEIWEI ZHANG LEI |
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Effective date of registration: 20140108 Address after: 516081 Guangdong province Huizhou city Dayawan district town o Nga Village Applicant after: Guangdong Dayawan Aquatic Products Experiment Center Applicant after: Sun Yat-sen University Address before: 516081 Guangdong province Huizhou city Dayawan district town o Nga Village Applicant before: Guangdong Dayawan Aquatic Products Experiment Center |
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