CN101796927B - Artificial breeding method of coregonus paled - Google Patents
Artificial breeding method of coregonus paled Download PDFInfo
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- CN101796927B CN101796927B CN2009101135382A CN200910113538A CN101796927B CN 101796927 B CN101796927 B CN 101796927B CN 2009101135382 A CN2009101135382 A CN 2009101135382A CN 200910113538 A CN200910113538 A CN 200910113538A CN 101796927 B CN101796927 B CN 101796927B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses an artificial breeding method of coregonus paled, in particular an artificial breeding method of cold water fishes, which belongs to the field of fishes. The method comprises six links of parent selection, fishing and transportation, maturity inspection, artificial egg taking, incubation and fry rearing, wherein the link of the artificial egg taking comprises five steps of egg taking, semen collection, fertilization, unsticking and counting. By using the method, the coregonus paled fishes bred in low-temperature mountain water in a mountain area in China becomes true, the eyedincubation rate of fries reaches 70 percent. By implementing the breeding method, the localization of coregonus paled fries can be thoroughly realized and the dependency on foreign countries can be gotten rid of.
Description
Technical field: artificial breeding method of coregonus paled of the present invention belongs to the fishery field, relates in particular to a kind of artificial cultivating method of cold water fish.
Background technology: pelyad fish, formal name used at school Coregonus peled, delicious, nutritious with its meat flavour, individual large, growing forms worldwide economy fish into important soon, it belongs to cold water migration fingerling, only originates in to the north of north latitude 17 degree as the ground such as Baikal of the state such as Russia.Application number is the application for a patent for invention of 03111786.4 denomination of invention for " a kind of method and apparatus that cultivates salmon ", disclose and utilized common fresh water, the Deep Underground water of mixing to extract, control water temperature through refrigeration plant, in the method for the medium and small aquifer cultivation salmon of common fresh water environment.Due to the technical protection doctrine; the Russia end discloses or reveals method of operating and the technical key point of relevant pelyad fish artificial breeding to China; from 1998-2001; 1,120 ten thousand of pelyad fish eyed eggses are introduced from this state continuously in Xinjiang; render to 2071 meters of height above sea level; water surface area reaches the Sayram Lake of 453 square kilometres, pays 1,200,000 yuans of the expenses of introducing a fine variety.Thisly from extensive introduction the overseas, transplant fish-egg, mode and our financial resources of artificial breeding pelyad fish can not adapt to fully, also make the numerous waters of the same type of China face a same difficult problem propagating artificially aspect the pelyad fish.
Summary of the invention: the object of the present invention is to provide a kind of method of utilizing the low Wenshan spring water artificial breeding pelyad fish in mountain area.
The objective of the invention is to be achieved by the following technical programs:
One, the parent selects: the Xinjiang Sayram Lake pelyad fish breeding that parental source is assert in the former breeding committee of country, meet the requirement of DB65/T2193, forbid that inbred offspring gives over to parent population, its answer physique healthy and strong, without disease, scar and deformity.More than three age raun 1200g, more than two age milter 600g; More than four age raun 1500g, more than three age milter 1000g.Female, male parent population mantissa proportioning is 1: 2.Parent population can use six full ages.
Two, the fishing for and transporting of parent: when the male parent population gonopore of light pressure front end, the adularescent seminal fluid flows out; Female parent population grows the IV end of term, cuts open the belly and sees have a small amount of ovum grain with ovary membrane, to separate, and can will both fish for out lake.Be transported to the flowing water pond at mountain spring place.When early November, the spring water temperature was down to 2 ℃ naturally, parent population is fished for.Select mature brood fish according to the ratio of female, male 1: 2, with bucket or the water transport of canvas bucket band, to adopting the ovum workshop, female, male parent population is put into respectively the storage pond, holding pond that is loaded with 2 ℃ of spring of water temperature.
Three, maturity inspection: female, milter are pulled out and select one by one with dragging for sea.Raun gently presses belly to have the ovum grain to flow out from gonopore, and the ovum grain of output is orange-yellow will not be adopted for the parent population of high-quality parent population, canescence ovum grain or overdone ovum; Milter gently presses gonopore front end adularescent seminal fluid to flow out.
Four, ovum artificial collection: the workshop of adopting ovum is equiped with the shading curtain, avoids direct light, and room temperature is between 0-12 ℃.Adopt the ovum instrument: white enamel basin, cotton towel, toilet paper, dried chicken feather, parent to be adopted, drag for sea, hatching bottle, except parent to be adopted and toilet paper, all the other instruments all need first with saturated brine, to soak more than 1 hour, to carry out disinfection before use, then clear water is rinsed well, dries stand-by.Dried chicken feather is fledging on Chicken Wings.
1, adopt ovum: every 3～5 tail rauns of female parent population that will meet above-mentioned body weight are one group, towel off dried fish body, dry gonopore with toilet paper, fish head-up, under afterbody is oblique, push from front to back fish belly and squeeze ovum facing to the white enamel basin, make the ovum grain along at the bottom of basin marginal flow engagement, avoid fish-egg from the too high injury of basin.Several fish-eggs of first extraction are discarded, if while band trace of blood fish-egg occurring, should stop immediately adopting ovum, every group of parent population time for oocyte collection is no more than 3 minutes.
2, semen collection: the semen collection mode is with adopting ovum, and the seminal fluid that the ovum of the ripe raun of every 1 tail need gather the ripe milter of 2 tails mates.
3, fertilization: when female fish-egg will soon have squeezed, the milter seminal fluid is clamp-oned rapidly on the fish-egg of having adopted, with dried chicken feather, stirred rapidly in 3 minutes, to the water that adds the same temperature of taking from the parent population storage pond, holding pond in basin, the water yield is advisable to cover 1/3 of ovum layer thickness.Stirred evenly standing 1 minute with dried chicken feather, the water that adds again 1/2 the same temperature of taking from the parent population storage pond, holding pond of ovum layer thickness, stir evenly standing 3-5 minute, with the water rinse of same temperature of taking from the parent population storage pond, holding pond, remove fouls such as fish scale, ight soil in basin, rinsing 5 times is to the limpid free from admixture of water quality in basin.Fill it up with the water of the same temperature of taking from the parent population storage pond, holding pond in basin, added a cover standing 120 minutes, make the abundant imbibition of ovum.
4, unsticking: prepared 100PPM tannic acid (C in front 15 minutes washing ovum
76H
52O
46) solution, wash fertilized egg 15-20 minute with it, add solution amount at every turn and be advisable with the 3cm that covers the ovum layer thickness, be divided into 5 flushings, each 34 minutes, solution added the dried chicken feather of rear use not stop to stir, and fertilized egg is fully contacted with solution, reaches the unsticking effect.Afterwards, add the water of the same temperature of taking from the parent population storage pond, holding pond in basin, the water yield is advisable just to cover the ovum face, repeatedly rinses 5 times, every 3 minutes, rinses one time, and each washing time 1 minute, wash away the white ovum in solution residual on ovum and basin.
5, counting: get 10mL fertilized egg, several 3 times repeatedly, draw the average ovum grain number of every milliliter, all fertilized eggs are measured total volume with the 1000mL measuring cup, multiply by every milliliter of ovum grain number and be the fertilized egg total amount, in its hatching bottle of packing into, 700,000 every bottle, start hatching.
Five, hatching: lucifuge is answered in the hatching workshop, and direct light can not be arranged, and hatching the bottle water injection rate needs constantly to increase according to the different phase of development of fertilized ova, and accumulated temperature in the time of 0-140 ℃, is hatched bottle water supply 2.2-2.4L/min for every; In the time of 140-220 ℃, every hatching bottle water supply 2.4-2.8L/min; When 220 ℃-membrane, every hatching bottle water supply 2.8-3.2L/min.Use the dead ovum in upper strata in siphonage sucking-off hatching bottle every day, the dried chicken feather of 3 to 4 use of every natural gift stirs hatches fertilized egg in bottle, prevents from pasting the bottle wall and forms a dead angle.Fertilized egg is sent out at the moment, embathed fertilized egg 15 minutes every 4 days with 1/2000 formalin solution, while embathing, first close the water inlet switch of hatching bottle, its interior water of sucking-off, pour formalin solution into along a bottle wall, liquid level surpasses the ovum face, stir gently with dried chicken feather, open the water inlet switch of hatching bottle after 15 minutes, be transferred to institute's water requirement.After sending out eye, in time merge the fertilized egg in each hatching bottle, clear out dead ovum.
Six, fry rearing: after the fry membrane, in time change in breeding groove and raise, the breeding groove depth of water is controlled at 30 centimetres, and breeding density is: in the time of below fish length 1.4-1.5 centimetre, be controlled at 400 tails/L, the water-soluble oxygen amount should be greater than 7mg/L.The opening material of fry is the halogen worm, measures fry length, weight every day,, according to 20% left and right of fry gross weight, divides and throws something and feeds for 6-8 time.Perform and anti-escape work, every daily siphonage soil pick-up 1 to 2 time, suck the foreign material such as the residual bait in breeding groove bottom, ight soil and dead fry, scrubbed every 2 hours anti-ly to escape net 1 time, ensures that current are unimpeded, observes at any time the fry situation of ingesting, monitoring physical and chemical factor situation of change.Treat that fry growth is long more than 1.5 centimetres to body, and when the shadow reflection was sensitive to external world, fry bred and can finish, and pulls out to put to fishpond and brings up.
Enforcement due to technique scheme, make and utilize the low Wenshan spring water in China mountain area to breed the pelyad fish to become a reality, as far back as June 16 calendar year 2001, Xinjiang Uygur Autonomous Regions Science and Technology Department once carried out appraisal of scientific and technological achievements to the artificial breeding method of coregonus paled of implementing a year, it is 50% that fish-egg is at that time sent out a rate, and the fry emergence rate is 10%.(01) No. 123 assay certificate of word that newly reflect thinks that the method has " good application prospect ", " achievement is on the leading domestic level ".Perfect through updating in recent years, fish-egg is sent out a rate and has been reached 65% now, and the fry emergence rate has reached 70%, adopts the method 8,500 ten thousand of artificial culture eyed eggses, fry 5,700 ten thousand tails.The popularization of pelyad seed has been carried out in success to domestic many waters, and has obtained good economic and social benefit, and the enforcement of this mating system has thoroughly realized the localization of pelyad fry kind, has broken away from external dependence.
Embodiment:
One, the parent selects: the Xinjiang Sayram Lake pelyad fish breeding that parental source is assert in the former breeding committee of country, meet the requirement of DB65/T2193, forbid that inbred offspring gives over to parent population, its answer physique healthy and strong, without disease, scar and deformity.More than three age raun 1200g, more than two age milter 600g; More than four age raun 1500g, more than three age milter 1000g.Female, male parent population mantissa proportioning is 1: 2.Parent population can use six full ages.
Two, the fishing for and transporting of parent: when the male parent population gonopore of light pressure front end, the adularescent seminal fluid flows out; Female parent population grows the IV end of term, cuts open the belly and sees have a small amount of ovum grain with ovary membrane, to separate, and can will both fish for out lake.Be transported to the flowing water pond that is positioned at Wenquan, Xinjiang bundle cloth hot spring place, Bu Re village, Le Mute township with live fish carrier, 1472 meters of this spring height above sea level, water temperature 1-19 ℃ throughout the year.The flowing water pond is outdoor cuboid, and the one end is put into spring, and the delivery port of the other end arranges aperture plate, blocks the parent population flight to flow out spring.When early November, the spring water temperature was down to 2 ℃ naturally, parent population is fished for.Select mature brood fish according to the ratio of female, male 1: 2, with bucket or the water transport of canvas bucket band, to adopting the ovum workshop, female, male parent population is put into respectively and is loaded with the storage pond, holding pond that water temperature is 2 ℃ of spring.
Three, maturity inspection: female, milter are pulled out and select one by one with dragging for sea.Raun gently presses belly to have the ovum grain to flow out from gonopore, and the ovum grain of output is orange-yellow will not be adopted for the parent population of high-quality parent population, canescence ovum grain or overdone ovum; Milter gently presses gonopore front end adularescent seminal fluid to flow out.
Four, ovum artificial collection: the workshop of adopting ovum is equiped with the shading curtain, avoids direct light, and room temperature is between 0-12 ℃.Adopt the ovum instrument: white enamel basin, cotton towel, toilet paper, dried chicken feather, parent to be adopted, drag for sea, hatching bottle, except parent to be adopted and toilet paper, all the other instruments all need first with saturated brine, to soak more than 1 hour, to carry out disinfection before use, then clear water is rinsed well, dries stand-by.Dried chicken feather is fledging on Chicken Wings.
1, adopt ovum: every 3～5 tail rauns of female parent population that will meet above-mentioned body weight are one group, towel off dried fish body, dry gonopore with toilet paper, fish head-up, under afterbody is oblique, push from front to back fish belly and squeeze ovum facing to the white enamel basin, make the ovum grain along at the bottom of basin marginal flow engagement, avoid fish-egg from the too high injury of basin.Several fish-eggs of first extraction are discarded, if while band trace of blood fish-egg occurring, should stop immediately adopting ovum, every group of parent population time for oocyte collection is no more than 3 minutes.
2, semen collection: the semen collection mode is with adopting ovum, and the seminal fluid that the ovum of the ripe raun of every 1 tail need gather the ripe milter of 2 tails mates.
3, fertilization: when female fish-egg will soon have squeezed, the milter seminal fluid is clamp-oned rapidly on the fish-egg of having adopted, with dried chicken feather, stirred rapidly in 3 minutes, to the water that adds the same temperature of taking from the parent population storage pond, holding pond in basin, the water yield is advisable to cover 1/3 of ovum layer thickness.Stirred evenly standing 1 minute with dried chicken feather, the water that adds again 1/2 the same temperature of taking from the parent population storage pond, holding pond of ovum layer thickness, stir evenly standing 3-5 minute, with the water rinse of same temperature of taking from the parent population storage pond, holding pond, remove fouls such as fish scale, ight soil in basin, rinsing 5 times is to the limpid free from admixture of water quality in basin.Fill it up with the water of the same temperature of taking from the parent population storage pond, holding pond in basin, added a cover standing 120 minutes, make the abundant imbibition of ovum.
4, unsticking: prepared 100PPM tannic acid (C in front 15 minutes washing ovum
76H
52O
46) solution, wash fertilized egg 15-20 minute with it, add solution amount at every turn and be advisable with the 3cm that covers the ovum layer thickness, be divided into 5 flushings, each 3-4 minute, solution add the dried chicken feather of rear use not stop to stir, and fertilized egg is fully contacted with solution, reach the unsticking effect.Afterwards, add the water of the same temperature of taking from the parent population storage pond, holding pond in basin, the water yield is advisable just to cover the ovum face, repeatedly rinses 5 times, every 3 minutes, rinses one time, and each washing time 1 minute, wash away the white ovum in solution residual on ovum and basin.
5, counting: get 10mL fertilized egg, several 3 times repeatedly, draw the average ovum grain number of every milliliter, all fertilized eggs are measured total volume with the 1000mL measuring cup, multiply by every milliliter of ovum grain number and be the fertilized egg total amount, in its hatching bottle of packing into, 700,000 every bottle, start hatching.It is 200920164517.9 that the hatching bottle is application number, and denomination of invention is cylinder for the middle and upper part described in " Coregonus peled hatching system ", and lower end is funnel shaped cavity glass hatching bottle.
Five, hatching: lucifuge is answered in the hatching workshop, and direct light can not be arranged.Hatching the bottle water injection rate needs constantly to increase according to the different phase of development of fertilized ova, and accumulated temperature in the time of 0-140 ℃, is hatched bottle water supply 2.2-2.4L/min for every; In the time of 140-220 ℃, every hatching bottle water supply 2.4-2.8L/min; When 220 ℃-membrane, every hatching bottle water supply 2.8-3.2L/min.Use the dead ovum in upper strata in siphonage sucking-off hatching bottle every day, the dried chicken feather of 3 to 4 use of every natural gift stirs hatches fertilized egg in bottle, prevents from pasting the bottle wall and forms a dead angle.Fertilized egg is sent out at the moment, embathed fertilized egg 15 minutes every 4 days with 1/2000 formalin solution, while embathing, first close the water inlet switch of hatching bottle, its interior water of sucking-off, pour formalin solution into along a bottle wall, liquid level surpasses the ovum face, stir gently with dried chicken feather, open the water inlet switch of hatching bottle after 15 minutes, be transferred to institute's water requirement.After sending out eye, in time merge the fertilized egg in each hatching bottle, clear out dead ovum.
Six, fry rearing: after the fry membrane, in time change in breeding groove and raise, the breeding groove depth of water is controlled at 30 centimetres, and breeding density is: in the time of below fish length 1.4-1.5 centimetre, be controlled at 400 tails/L, the water-soluble oxygen amount should be greater than 7mg/L.The opening material of fry is the halogen worm, measures fry length, weight every day,, according to 20% left and right of fry gross weight, divides and throws something and feeds for 6-8 time, to guarantee that fry has enough, is as the criterion.Perform and prevent escaping work, every daily siphonage soil pick-up 1 to 2 time, suck the foreign material such as the residual bait in breeding groove bottom, ight soil and dead fry, scrubbed every 2 hours and anti-escape net 1 time, ensure that current are unimpeded, observe at any time the fry situation of ingesting, monitoring physical and chemical factor situation of change, pinpoint the problems and in time process.Treat that fry growth is long more than 1.5 centimetres to body, and when the shadow reflection was sensitive to external world, fry bred and can finish, and pulls out to put to fishpond and brings up.
Claims (5)
1. artificial breeding method of coregonus paled, it is characterized in that: concrete link and the step of the method are as follows:
One, the parent selects: the Xinjiang Sayram Lake pelyad fish breeding that parental source is assert in the former breeding committee of country, meet the requirement of DB65/T2193, forbid that inbred offspring gives over to parent population, its answer physique healthy and strong, without disease, scar and deformity, more than three age raun 1200g, more than two age milter 600g; More than four age raun 1500g, more than three age milter 1000g, female, male parent population mantissa proportioning is 1: 2, and parent population can use six full ages;
Two, the fishing for and transporting of parent: when the male parent population gonopore of light pressure front end, the adularescent seminal fluid flows out; Female parent population grows the IV end of term, cut open the belly and see have a small amount of ovum grain with ovary membrane, to separate, lake can will both be fished for out, be transported to the flowing water pond at mountain spring place, when early November, the spring water temperature was down to 2 ℃ naturally, parent population is fished for, select mature brood fish according to ratio female, male 1: 2, to adopting the ovum workshop, female, male parent population is put into respectively the storage pond, holding pond that is loaded with 2 ℃ of spring of water temperature with bucket or the water transport of canvas bucket band;
Three, maturity inspection: with dragging for sea, female, milter are pulled out and select one by one, raun gently presses belly to have the ovum grain to flow out from gonopore, and the ovum grain of output is orange-yellow will not be adopted for the parent population of high-quality parent population, canescence ovum grain or overdone ovum; Milter gently presses gonopore front end adularescent seminal fluid to flow out;
Four, ovum artificial collection: the workshop of adopting ovum is equiped with the shading curtain, avoid direct light, room temperature is between 0-12 ℃, adopt the ovum instrument: white enamel basin, cotton towel, toilet paper, dried chicken feather, parent to be adopted, drag for sea, hatching bottle, except parent to be adopted and toilet paper, all the other instruments all need first with saturated brine, to soak more than 1 hour before use, to carry out disinfection, then clear water is rinsed well, dries stand-byly, and dried chicken feather is fledging on Chicken Wings;
1, adopt ovum: every 3～5 tail rauns of female parent population that will meet above-mentioned body weight are one group, towel off dried fish body, with toilet paper, dry gonopore, fish head-up, under afterbody is oblique, push from front to back fish belly and squeeze ovum facing to the white enamel basin, make the ovum grain along at the bottom of basin marginal flow engagement, avoid fish-egg from the too high injury of basin, several fish-eggs of first extraction are discarded, if while band trace of blood fish-egg occurring, should stop immediately adopting ovum, every group of parent population time for oocyte collection is no more than 3 minutes;
2. semen collection: the semen collection mode is with adopting ovum, and the seminal fluid that the ovum of the ripe raun of every 1 tail need gather the ripe milter of 2 tails mates;
3. fertilization: when female fish-egg will soon have squeezed, the milter seminal fluid is clamp-oned rapidly on the fish-egg of having adopted, stir rapidly in 3 minutes with dried chicken feather, to the water that adds the same temperature of taking from the parent population storage pond, holding pond in basin, the water yield covers 1/3 of ovum layer thickness, stirred evenly standing 1 minute with dried chicken feather, the water that adds again 1/2 the same temperature of taking from the parent population storage pond, holding pond of ovum layer thickness, stir evenly standing 3-5 minute, remove fish scale in basin with the water rinse of the same temperature of taking from the parent population storage pond, holding pond, the fouls such as ight soil, rinsing 5 times is to the limpid free from admixture of water quality in basin, fill it up with the water of the same temperature of taking from the parent population storage pond, holding pond in basin, added a cover standing 120 minutes, make the abundant imbibition of ovum,
4. unsticking: prepared 100PPM tannic acid (C in front 15 minutes washing ovum
76H
52O
46) solution, wash fertilized egg 15-20 minute with it, add solution amount at every turn and cover the 3cm of ovum layer thickness, be divided into 5 flushings, each 3-4 minute, solution add the dried chicken feather of rear use not stop to stir, fertilized egg is fully contacted with solution, reach the unsticking effect, afterwards, the water that adds the same temperature of taking from the parent population storage pond, holding pond in basin, the water yield has just covered the ovum face, repeatedly rinses 5 times, every 3 minutes, rinses one time, each washing time 1 minute, wash away the white ovum in solution residual on ovum and basin;
5. count: get 10mL fertilized egg, several 3 times repeatedly, draw the average ovum grain number of every milliliter, all fertilized eggs are measured total volume with the 1000mL measuring cup, multiply by every milliliter of ovum grain number and be the fertilized egg total amount, in its hatching bottle of packing into, 700,000 every bottle, start hatching;
five, hatching: lucifuge is answered in the hatching workshop, and direct light can not be arranged, and hatching the bottle water injection rate needs constantly to increase according to the different phase of development of fertilized ova, and accumulated temperature in the time of 0-140 ℃, is hatched bottle water supply 2.2-2.4L/min for every, in the time of 140-220 ℃, every hatching bottle water supply 2.4-2.8L/min, when 220 ℃-membrane, every hatching bottle water supply 2.83.2L/min, hatch bottle interior upper strata dead ovum with the siphonage sucking-off every day, the dried chicken feather of 3 to 4 use of every natural gift stirs fertilized egg in the hatching bottle, prevent from pasting the bottle wall and form dead angle, fertilized egg is sent out at the moment, embathed fertilized egg 15 minutes every 4 days with 1/2000 formalin solution, while embathing, first close the water inlet switch of hatching bottle, its interior water of sucking-off, pour formalin solution into along the bottle wall, liquid level surpasses the ovum face, stir gently with dried chicken feather, open the water inlet switch of hatching bottle after 15 minutes, be transferred to institute's water requirement, after sending out eye, in time merge the fertilized egg in each hatching bottle, clear out dead ovum,
six, fry rearing: after the fry membrane, in time change in breeding groove and raise, the breeding groove depth of water is controlled at 30 centimetres, breeding density is: in the time of below fish length 1.4-1.5 centimetre, be controlled at 400 tails/L, the water-soluble oxygen amount should be greater than 7mg/L, the opening material of fry is the halogen worm, measure fry length every day, weight, according to 20% of fry gross weight, divide and throw something and feed for 6-8 time, perform the anti-work of escaping, every daily siphonage soil pick-up 1 to 2 time, suck the residual bait in breeding groove bottom, the foreign material such as ight soil and dead fry, scrubbed every 2 hours and prevent escaping net 1 time, the guarantee current are unimpeded, observe at any time the fry situation of ingesting, monitoring physical and chemical factor situation of change, treat that fry growth is long more than 1.5 centimetres to body, and when shadow reflection is sensitive to external world, fry breeds and can finish, pull out to put to fishpond and bring up.
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| CN105052787B (en) * | 2015-07-29 | 2018-01-05 | 通威股份有限公司 | Pelyad fry rearing method |
| CN105724292A (en) * | 2016-03-07 | 2016-07-06 | 五河县金满塘生态种养殖家庭农场 | Artificial insemination breeding method for protosalanx hyalocranius |
| CN105706976A (en) * | 2016-03-07 | 2016-06-29 | 五河县金满塘生态种养殖家庭农场 | Artificial fertilization breeding method for loaches |
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| CN111919799A (en) * | 2020-07-13 | 2020-11-13 | 浙江省海洋水产养殖研究所 | Method for debonding viscous fertilized eggs of marine fishes |
| CN112167118A (en) * | 2020-10-12 | 2021-01-05 | 新疆维吾尔自治区水产科学研究所 | Artificial propagation method of zier whitefish |
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| "高白鲑人工繁育技术";孔令杰;《渔业致富指南》;20090225;44-45页 * |
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