CN104145866B - The method of Leusiscus brandti artificial breeding, enhancement releasing - Google Patents

The method of Leusiscus brandti artificial breeding, enhancement releasing Download PDF

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CN104145866B
CN104145866B CN201410418653.1A CN201410418653A CN104145866B CN 104145866 B CN104145866 B CN 104145866B CN 201410418653 A CN201410418653 A CN 201410418653A CN 104145866 B CN104145866 B CN 104145866B
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hatchery
incubation tank
internal box
fry
leusiscus
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CN104145866A (en
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王维坤
姜殿波
刘菊英
马立新
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王维坤
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The method of Leusiscus brandti artificial breeding, enhancement releasing, it relates to a kind of method of artificial breeding, enhancement releasing fish.The present invention is in order to solve incubation rate and the low technical problem of survival rate.This method is as follows: one, parent population is adopted and catches; Two, fish-egg collection, fertilization, unsticking; Three, incubating oosperm, fry rearing; Four, fry is released.The inventive method adds the dissolved oxygen in incubating oosperm process by the mode of aeration bottom employing incubation tank, and aeration makes the viscosity of fertilized egg reduce, effectively can solve prior art incubation rate, deficiency that survival rate is low, the average daily incubation rate of the inventive method is adopted to reach more than 80%, emergence rate reaches more than 90%, and survival rate is to 95%.The invention belongs to the field of artificial breeding, enhancement releasing fish.

Description

The method of Leusiscus brandti artificial breeding, enhancement releasing
Technical field
The present invention relates to a kind of method of artificial breeding, enhancement releasing fish.
Background technology
Leusiscus brandti belongs to bony fish, Cyprinidae, Leuciscinae, leuciscus, is the unique anadromous migration fish of Cyprinidae, is distributed in Japanese's seawater system of North Pacific in ground, only to distribute the Suifenhe, TUMENJIANG RIVER in China.This kind fry hatches in freshet, goes downstream in offshore waters growth afterwards, fattens, after gonadal maturation, trace back annual April-June river and on get back to fresh water, egg reproduction on the mouth of the whistle, anxious beach, returns to marine site after adult fish lays eggs immediately, so gain the name " beachhead fish ".Leusiscus brandti is well-proportioned, body colour is bright-coloured, delicious flavour, has very high economic worth.Annual spawning season, adult fish anadromous migration forms " fishing season ", is the best opportunity of fishing for.In the large major production areas of China two: the Suifenhe and TUMENJIANG RIVER, Leusiscus brandti has become local terrestrial reference product, be spring product fish have a taste of a delicacy, the optimal selection of presenting friends, and its market price also goes up year after year, can reach 100 yuan one in some time.
But, manually fish in a large number, especially adult fish lay eggs before fish for the Sustainable Development and Utilization having had a strong impact on Leusiscus brandti fishery resources.In order to address this problem, from the eighties in last century, the people such as Heilungkiang aquatic products research institute Dong Chongzhi researcher are together with applicant, the technology of a set of Leusiscus brandti artificial breeding hatching, seed rearing, enhancement releasing is found out in research, and continue practice in the Suifenhe, define a set of relatively complete technical system, alleviate the problem that wild seed quantity reduces, fishery resources decline of fishing for and causing to a certain extent.This technology utilizes adopts the ripe adult fish of the wildness of catching as parent population, the Suifenhe nature water surface arranges the artificial incubation that comparatively original wooden incubator box carries out fish-egg, substantially flow process is: fish for that body colour is bright-coloured, belly expands and the adult fish of softness as parent population, manually extrude ovum grain and seminal fluid, dry method artificial insemination obtains fertilized egg; Be formulated to the rectangle wooden case of wide 80 centimetres, long 100 centimetres with plank, on the side face drilling of wooden case diameter 0.5 centimetre be uniformly distributed ommatidium, the rain figured cloth access fertilized fish roe of maximum order number is laid in bottom.Wooden case is arranged in river, utilizes the environmental condition such as natural temperature, current to hatch.Wooden case can be swum out of voluntarily after going out the flat trip of the fry after shell, utilize nature bait to grow.
Although the method is through putting into practice for many years, Leusiscus brandti seed quantity can be increased, but at many ins and outs existing defects with not enough, cause fertilized egg survival rate, emergence rate and survival rate lower, can not adapt to the demand growing to its fishery resources.Deficiency comprise following some:
1. Leusiscus brandti ovum belongs to viscosity disorderly, meets water and produces viscosity, can comparatively firmly be bonded on rain figured cloth after artificial insemination, when fish-egg quantity is larger, after pour rain figured cloth into fish-egg can constantly cover bottom fish-egg, form the thick fish-egg lump reaching several centimetres.In hatching process, the water that bottom ovum grain touches is less, very easily anoxic lethal cases occurs; Secondly go out the shell stage fry, be crushed on the fry not easily broken shell of bottom, be easily trapped in lump after going out shell and cannot float; In addition stick to each other and also can be easy to the extensive relay of water mold, cause fish-egg Large Scale Death.
2. environmental condition is wayward.The method uses wooden case to utilize Suifenhe natural water body to hatch, and because 4-6 month river temperature is lower, causes the incubation period longer, and lethality increases; Meanwhile, the key factors such as river oxygen content in water, temperature affect comparatively large by Changes in weather, acute variation easily occurs, too increases the uncertain factor of hatching, easily cause the massive mortality of fish-egg; In addition water body is excessive, uncontrollable change of water quality, manually can not sterilize and avoid water mold to occur.
3. namely fry faces the challenge of wild environment after going out shell.After fry goes out shell, perfect condition is issued to flat free state, physically fit rear wooden case of just swimming out of is ingested, but a large amount of fry is when just going out shell in practice, physique weakness cannot be moved about and namely be gone out incubator box by current, causes it very easily preyed on by other biological in wild environment or rush lethal to brief talking injured anoxic on rock.
4. cannot accurately estimate fry output.In wooden case, a large amount of fry is from being advanced in river, fry output cannot be calculated, can only be measured by fertilized egg and rule of thumb estimate emergence rate and survival rate, estimate output roughly, forecast assessment cannot be carried out for enhancement releasing working effect accurately, ensure the stable of fishery resources from now on.
Above problem seriously constrains efficiently carrying out of Leusiscus brandti artificial incubation work, make hatch fish roe rate survival rate lower, fry output cannot be ensured.
Summary of the invention
The object of the invention is, in order to solve incubation rate and the low technical problem of survival rate, to provide a kind of method of Leusiscus brandti artificial breeding, enhancement releasing.
The method of Leusiscus brandti artificial breeding, enhancement releasing is carried out according to following steps:
One, parent population is adopted and catches: mid-May to mid-June adopt catch gonad development be the V phase 3 age raun and male, then put into respectively and store up cage and store up;
Two, fish-egg gathers, fertilization, unsticking: under the condition of temperature less than 20 DEG C, extrusion is adopted to gather mature egg grain, every ovum basin of only adopting gathers 10 ~ 15 tail raun mature egg grain 20 ~ 300,000, gather 9 ~ 12 tail milter seminal fluid to adopting in ovum basin, fully stirring with writing brush makes seminal fluid evenly be wrapped in around mature egg grain, leave standstill after 2 minutes, to adopting injected clear water in ovum basin, stirring makes activation of spermatozoa, complete fertilization, then adding tannic acid makes tannic acid concentration in mixed liquor be 1g/L, add tannic acid to rinse 4 ~ 5 times with clear water after one minute, then fertilized egg is moved in tank and expand half an hour, by fertilized egg mass concentration be again 0.2% methylene blue solution soak 20 minutes,
Three, incubating oosperm, fry rearing: the fertilized egg through step 2 process is received the hatching 7 ~ 12 days in the hatchery that water temperature is 14 ~ 22 DEG C, the depth of water is 30cm, dissolved oxygen is more than 10.5mg/L, transparency is 25 ~ 30cm, flow velocity is 0.7 ~ 1.0m/s of the ovum amount of receiving of 20 ~ 300,000, ovum according to each incubation tank (7), and then flow velocity is down to 0.3 ~ 0.4m/s hatching 7 ~ 12 days;
Four, fry is released:
After fry floats flat trip, the ratio of 1000g is added according to every cube of water body, to splash whole hatchery with the egg yolk mixture of soya-bean milk and grinding, 1 day 2 times, and coordinate fairy shrimp of feeding, feed 2 ~ 3 days, the fry net utilizing 80 mesh sieve thin,tough silk to make, fishes for the fry of 5 ~ 10 grams of dry weights, check number, calculate single fry heavy, weigh all fry dry weights in hatchery, calculate gross yield, after obtaining output, select the morning of the maximum air temperature 19 ~ 20 DEG C, calm or gentle breeze, at river unhurried current, fry is poured in river by place slowly, completes enhancement releasing.
Hatchery described in step 3 is by water inlet, air pump, air-supplying valve, appendix, aeration plate, hatchery outer case wallboard, hatchery outer case base plate, incubation tank forms, hatchery outer case wallboard and hatchery outer case base plate surround and form hatchery outer case, outer case inside, hatchery is provided with incubation tank, described incubation tank is by incubation tank internal box wallboard, incubation tank internal box base plate, incubation tank internal box swash plate connects to form, described incubation tank internal box base plate and incubation tank internal box swash plate form obtuse angle, the vertical section of incubation tank is that bottom is short, the inverted trapezoidal of upper bottom side length, incubation tank internal box swash plate top is provided with incubation tank internal box swash plate apopore, incubation tank internal box base plate is provided with incubation tank internal box base plate inlet opening, hatchery internal box baffle plate to be positioned at below incubation tank internal box swash plate with hatchery outer case base plate and incubation tank internal box base plate is airtight is connected, aeration plate is positioned at the space that incubation tank internal box base plate, hatchery outer case base plate and hatchery internal box baffle plate are formed, and aeration plate is parallel with the bottom of incubation tank, aeration plate is connected with air pump by appendix, and the appendix branch being communicated with each aeration plate is provided with an air-supplying valve.
Outer case inside, described hatchery is provided with incubation tank, and the number of incubation tank is more than or equal to 4.
Raun fish length 29cm, more than body weight 0.4kg in step one; Milter fish length 26cm, more than body weight 0.25kg, and extrusion flow seminal fluid.Store up in step one in cage and store up density and be 40 Wei/㎡.
The inventive method adds the dissolved oxygen in incubating oosperm process by the mode of aeration bottom employing incubation tank, and aeration stirs water body and makes fertilized egg not easily adhesion, effectively can solve prior art incubation rate, deficiency that survival rate is low, the average daily incubation rate of the inventive method is adopted to reach more than 80%, emergence rate reaches more than 90%, and survival rate is to 95%.
The present invention was through practice in continuous 14 years, and fish-egg eyespot phase survival rate is compared existing method and improve more than 65%, and incubation rate improves more than 60%, and survival rate improves more than 70%.Production efficiency improves greatly, in addition, due to the controllability of environmental factor, can increase hatch fish roe density, improve gross yield, between 14 years, average postlarva output reaches more than 5,000 ten thousand tails, output increased nearly 80%.
Accompanying drawing explanation
Fig. 1 is the structural representation of the present invention hatchery used.
Embodiment
Technical solution of the present invention is not limited to following cited embodiment, also comprises any combination between each embodiment.
Embodiment one: in present embodiment, the method for Leusiscus brandti artificial breeding, enhancement releasing is carried out according to following steps:
One, parent population is adopted and catches: mid-May to mid-June adopt catch gonad development be the V phase 3 age raun and male, then put into respectively and store up cage and store up;
Two, fish-egg gathers, fertilization, unsticking: under the condition of temperature less than 20 DEG C, extrusion is adopted to gather mature egg grain, every ovum basin of only adopting gathers 10 ~ 15 tail raun mature egg grain 20 ~ 300,000, gather 9 ~ 12 tail milter seminal fluid to adopting in ovum basin, fully stirring with writing brush makes seminal fluid evenly be wrapped in around mature egg grain, leave standstill after 2 minutes, to adopting injected clear water in ovum basin, stirring makes activation of spermatozoa, complete fertilization, then adding tannic acid makes tannic acid concentration in mixed liquor be 1g/L, add tannic acid, rinse 4 ~ 5 times with clear water after one minute, then fertilized egg is moved in tank and expand half an hour, by fertilized egg mass concentration be again 0.2% methylene blue solution soak 20 minutes,
Three, incubating oosperm, fry rearing: the fertilized egg through step 2 process is received the hatching 7 ~ 12 days in the hatchery that water temperature is 14 ~ 22 DEG C, the depth of water is 30cm, dissolved oxygen is more than 10.5mg/L, transparency is 25 ~ 30cm, flow velocity is 0.7 ~ 1.0m/s of the ovum amount of receiving of 20 ~ 300,000, ovum according to each incubation tank (7), and then flow velocity is down to 0.3 ~ 0.4m/s hatching 7 ~ 12 days;
Four, fry is released:
After fry floats flat trip, the ratio of 1000g is added according to every cube of water body, to splash whole hatchery with the egg yolk mixture of soya-bean milk and grinding, 1 day 2 times, and coordinate fairy shrimp of feeding, feed 2 ~ 3 days, the fry net utilizing 80 mesh sieve thin,tough silk to make, fishes for the fry of 5 ~ 10 grams of dry weights, check number, calculate single fry heavy, weigh all fry dry weights in hatchery, calculate gross yield, after obtaining output, select the morning of the maximum air temperature 19 ~ 20 DEG C, calm or gentle breeze, at river unhurried current, fry is poured in river by place slowly, completes enhancement releasing.
In the egg yolk mixture of soya-bean milk and grinding in present embodiment step 4, the ratio of soya-bean milk and egg yolk is 100g Dou Jiang ﹕ 1 yolk.
Described in present embodiment step 3, transparency is recorded by transparent scale.
Embodiment two: often only adopting ovum basin gathers 12 raun mature egg grain 250,000 unlike in step 2 in step 2 for present embodiment and embodiment one, gathers 11 tail milter seminal fluid to adopting in ovum basin.Other is identical with embodiment one.
Embodiment three: one of present embodiment and embodiment one or two hatch 8 days unlike in step 3 in the hatchery that water temperature is 15 ~ 20 DEG C, the depth of water is 30cm, dissolved oxygen is 10.5mg/L, transparency is 26cm, flow velocity is 0.8m/s, and then flow velocity is down to 0.3m/s and hatches 8 days.Other is identical with one of embodiment one or two.
Embodiment four: flow velocity unlike hatching 10 days in the hatchery that water temperature is 18 DEG C, the depth of water is 30cm, dissolved oxygen is more than 10.5mg/L, transparency is 28cm, flow velocity is 0.9m/s in step 3, and then is down to 0.4m/s and is hatched 10 days by one of present embodiment and embodiment one to three.Other is identical with one of embodiment one to three.
Embodiment five: one of present embodiment and embodiment one to four unlike the hatchery described in step 3 by water inlet 1, air pump 2, air-supplying valve 3, appendix 4, aeration plate 5, hatchery outer case wallboard 6-1, hatchery outer case base plate 6-3, incubation tank 7 forms, hatchery outer case wallboard 6-1 and hatchery outer case base plate 6-3 surrounds and forms hatchery outer case, outer case inside, hatchery is provided with incubation tank 7, described incubation tank 7 is by incubation tank internal box wallboard 6-2, incubation tank internal box base plate 6-4, incubation tank internal box swash plate 6-6 connects to form, described incubation tank internal box base plate 6-4 and incubation tank internal box swash plate 6-6 forms obtuse angle, the vertical section of incubation tank 7 is that bottom is short, the inverted trapezoidal of upper bottom side length, incubation tank internal box swash plate 6-6 top is provided with incubation tank internal box swash plate apopore 6-61, incubation tank internal box base plate 6-4 is provided with incubation tank internal box base plate inlet opening 6-41, hatchery internal box baffle plate 6-5 to be positioned at below incubation tank internal box swash plate 6-6 with hatchery outer case base plate 6-3 and incubation tank internal box base plate 6-4 is airtight is connected, aeration plate 5 is positioned at the space that incubation tank internal box base plate 6-4, hatchery outer case base plate 6-3 and hatchery internal box baffle plate 6-5 are formed, and aeration plate 5 is parallel with the bottom of incubation tank 7, aeration plate 5 is connected with air pump 2 by appendix 4, and appendix 4 branch being communicated with each aeration plate 5 is provided with an air-supplying valve 3.Other is identical with one of embodiment one to four.
The inverted trapezoidal being short, the upper bottom side length in bottom by incubation tank internal box wallboard 6-2, incubation tank internal box base plate 6-4 and incubation tank internal box swash plate 6-6 region vertical section, besieged city (i.e. the vertical section of incubation tank 7).
Embodiment six: present embodiment and embodiment five are provided with incubation tank 7 unlike outer case inside, described hatchery, and the number of incubation tank 7 is more than or equal to 4.Other is identical with embodiment five.
Embodiment seven: present embodiment and embodiment five are unlike raun fish length 29cm, more than body weight 0.4kg in step one; Milter fish length 26cm, more than body weight 0.25kg, and extrusion flow seminal fluid.Other is identical with embodiment five.
Embodiment eight: present embodiment and embodiment five are stored up density be 40 Wei/㎡ unlike being stored up in step one in cage.Other is identical with embodiment five.
Adopt following experimental verification effect of the present invention:
Experiment one:
The method of Leusiscus brandti artificial breeding, enhancement releasing is carried out according to following steps:
Between the 4-6 months in 2014, carry out Leusiscus brandti artificial fecundation at river, Suifenhe Dongning County section tissue and to release experiment, adopt old technical system and neoteric technical system, the comparative experiments of system has been carried out to parameters.Result is as follows:
One, parent population is adopted and catches: in mid-May to mid-June, select 3 fish in age female fish length 29cm, fish that the above gonad development of body weight 0.4kg is the V phase is female parent population, the fish of the male fish length 26cm of 3 fish in age, body weight 0.25kg above gonad development V phase, extrusion flow seminal fluid is male parent population, adopt altogether and catch parent population 12648 tail, wherein female-male proportion is 2.0: 1.0, the female parent population chosen and male parent population are put into respectively and stores up cage (storing up cage is rectangle, 2m × 1.5m × 1m, iron cage iron sieve is welded) in store up, storing up density is 40 Wei/㎡;
Two, fish-egg gathers, fertilization, unsticking: under the condition of temperature less than 20 DEG C, extrusion is adopted to gather mature egg grain, often only adopt ovum basin (diameter 32cm, dark 13cm) gather 10719.9 ten thousand ovum, wherein 7,620 ten thousand as Leusiscus brandti artificial breeding, the hatching material of the method for enhancement releasing (all the other 3,000 ten thousand as a comparison test material), gather milter seminal fluid to adopting in ovum basin, fully stirring with writing brush makes seminal fluid evenly be wrapped in around mature egg grain, leave standstill after 2 minutes, to adopting injected clear water in ovum basin, stirring makes activation of spermatozoa, being fertilized, (now fish-egg produces viscosity, mutual attachment and stick at adopt ovum tub wall), then adding tannic acid makes tannic acid concentration in mixed liquor be 1g/L, , add tannic acid ... 4 times are rinsed with clear water after time, then expand fertilized egg immigration grain (fiberglass, 40 × 40 × 15cm) expand half an hour, by fertilized egg mass concentration be again 0.2% methylene blue solution soak 20 minutes (preventing water mo(u)ld from infecting),
Three, incubating oosperm, fry rearing:
Be 18 ~ 22 DEG C of (average out to 19 DEG C by water temperature, ) within 24 hours, tan by the sun the phreatic water of heating through open water mud sump and enter hatchery by water inlet 1, water enters incubation tank 7 by incubation tank internal box base plate inlet opening 6-41, when the water level in first incubation tank 7 exceedes incubation tank internal box swash plate apopore 6-61, current flow into next incubation tank 7 by incubation tank internal box swash plate apopore 6-61, until all incubation tanks are full of water, 7,620 ten thousand fertilized eggs through step 2 process are put in incubation tank 7, and open air-supplying valve 3, open air pump 2 and carry out aeration by aeration plate 5, making oxygen enter dissolved oxygen in incubation tank 7 to incubation tank 7 by incubation tank internal box swash plate apopore 6-61 is 10.5mg/L, then be 10.5mg/L at dissolved oxygen, the depth of water is 30cm, transparency is 30cm, flow velocity be 1.0m/s hatchery in hatching fertilized egg 7 days, and then flow velocity is down to 0.3m/s hatches 7 days, hatching accumulated temperature is 70 DEG C of days,
Four, fry is released:
After fry floats flat trip, the ratio of 1000g is added according to every cube of water body, to splash whole hatchery with the egg yolk mixture of soya-bean milk and grinding, 1 day 2 times, and coordinate fairy shrimp of feeding, feed 3 days, the fry net utilizing 80 mesh sieve thin,tough silk to make, fishes for the fry of 5 grams of dry weights, check number, calculate single fry heavy, weigh all fry dry weights in hatchery, calculate gross yield, after obtaining output, select the morning of the maximum air temperature 19 ~ 20 DEG C, calm or gentle breeze, at river unhurried current, fry is poured in river by place slowly, completes enhancement releasing.
Contrast experiment:
The Suifenhe nature water surface arranges the artificial incubation that wooden incubator box carries out fish-egg, wide 80 centimetres, long 100 centimetres rectangle wooden case is formulated to plank, on the side face drilling of wooden case diameter 0.5 centimetre be uniformly distributed ommatidium, the rain figured cloth access fertilized fish roe of maximum order number is laid in bottom.Wooden case is arranged in river, drops into 3,000 ten thousand fish-eggs, gather milter seminal fluid, in river, 8 days incubation periods hatching accumulated temperature is 120 DEG C of days.
Contrast experiment from above:
Adopt the method fish-egg fertilization rate reached of Leusiscus brandti artificial breeding, enhancement releasing to 95%, the ratio that fish-egg infects water mold is no more than 5%; Membrane prelarva total length 6.5mm (6.3 ~ 7.2mm), body weight 3.73mg, average percentage hatch rate is 81.0%, and May 30 temporarily supported prelarva 5972.5 ten thousand tail, and total length 1.05 ~ 1.25cm behind June 4 prelarva upstream, enters postlarva 5972.5 ten thousand tail.Cultivated through 3 days, postlarva total length reaches 12.5 ~ 15.0mm, and the postlarva amount of releasing reaches more than 3,000 ten thousand tails.
Contrast experiment's fish-egg fertilization rate is 68%, and have the fish-egg of 40% ~ 50% infect water mold or surrounded by water mold, average percentage hatch rate is less than 40%, and total length 0.75 ~ 1.15cm behind prelarva upstream, postlarva mantissa is through being estimated as 800 ~ 9,000,000 tails.Cultivated through 3 days, postlarva total length 7.5 ~ 15mm, postlarva to be preyed on by large fish and the situation of natural death transfers flow about 8,000,000 tails not calculating.

Claims (7)

1. the method for Leusiscus brandti artificial breeding, enhancement releasing, is characterized in that: the method for Leusiscus brandti artificial breeding, enhancement releasing is carried out according to following steps:
One, parent population is adopted and catches: mid-May to mid-June adopt catch gonad development be the V phase 3 age raun and milter, then put into respectively and store up cage and store up;
Two, fish-egg gathers, fertilization, unsticking: under the condition of temperature less than 20 DEG C, extrusion is adopted to gather mature egg grain, every ovum basin of only adopting gathers 10 ~ 15 tail raun mature egg grain 20 ~ 300,000, gather 9 ~ 12 tail milter seminal fluid to adopting in ovum basin, fully stirring with writing brush makes seminal fluid evenly be wrapped in around mature egg grain, leave standstill after 2 minutes, to adopting injected clear water in ovum basin, stirring makes activation of spermatozoa, complete fertilization, then adding tannic acid makes tannic acid concentration in mixed liquor be 1g/L, add tannic acid to rinse 4 ~ 5 times with clear water after one minute, then fertilized egg is moved in tank and expand half an hour, by fertilized egg mass concentration be again 0.2% methylene blue solution soak 20 minutes,
Three, incubating oosperm, fry rearing: the fertilized egg through step 2 process is received the hatching 7 ~ 12 days in the hatchery that water temperature is 14 ~ 22 DEG C, the depth of water is 30cm, dissolved oxygen is more than 10.5mg/L, transparency is 25 ~ 30cm, flow velocity is 0.7 ~ 1.0m/s of the ovum amount of receiving of 20 ~ 300,000, ovum according to each incubation tank (7), and then flow velocity is down to 0.3 ~ 0.4m/s hatching 7 ~ 12 days;
Four, fry is released:
After fry floats flat trip, the ratio of 1000g is added according to every cube of water body, to splash whole hatchery with the egg yolk mixture of soya-bean milk and grinding, 1 day 2 times, and coordinate fairy shrimp of feeding, feed 2 ~ 3 days, the fry net utilizing 80 mesh sieve thin,tough silk to make, fishes for the fry of 5 ~ 10 grams of dry weights, check number, calculate single fry heavy, weigh all fry dry weights in hatchery, calculate gross yield, after obtaining output, select the morning of the maximum air temperature 19 ~ 20 DEG C, calm or gentle breeze, at river unhurried current, fry is poured in river by place slowly, completes enhancement releasing;
Wherein, hatchery described in step 3 is by water inlet (1), air pump (2), air-supplying valve (3), appendix (4), aeration plate (5), hatchery outer case wallboard (6-1), hatchery outer case base plate (6-3), incubation tank (7) forms, hatchery outer case wallboard (6-1) and hatchery outer case base plate (6-3) are surrounded and are formed hatchery outer case, outer case inside, hatchery is provided with incubation tank (7), described incubation tank (7) is by incubation tank internal box wallboard (6-2), incubation tank internal box base plate (6-4), incubation tank internal box swash plate (6-6) connects to form, described incubation tank internal box base plate (6-4) and incubation tank internal box swash plate (6-6) form obtuse angle, the vertical section of incubation tank (7) is that bottom is short, the inverted trapezoidal of upper bottom side length, incubation tank internal box swash plate (6-6) top is provided with incubation tank internal box swash plate apopore (6-61), incubation tank internal box base plate (6-4) is provided with incubation tank internal box base plate inlet opening (6-41), hatchery internal box baffle plate (6-5) is positioned at incubation tank internal box swash plate (6-6) below with hatchery outer case base plate (6-3) with incubation tank internal box base plate (6-4) is airtight is connected, the space that aeration plate (5) is positioned at incubation tank internal box base plate (6-4), hatchery outer case base plate (6-3) is formed with hatchery internal box baffle plate (6-5), and aeration plate (5) is parallel with the bottom of incubation tank (7), aeration plate (5) is connected with air pump (2) by appendix (4), and appendix (4) branch being communicated with each aeration plate (5) is provided with an air-supplying valve (3).
2. the method for Leusiscus brandti artificial breeding, enhancement releasing according to claim 1, is characterized in that:
In step 2, often only adopting ovum basin gathers 12 tail raun mature egg grain 250,000, gathers 11 tail milter seminal fluid to adopting in ovum basin.
3. the method for Leusiscus brandti artificial breeding, enhancement releasing according to claim 1, is characterized in that:
Hatching 8 days in the hatchery that water temperature is 15 ~ 20 DEG C, the depth of water is 30cm, dissolved oxygen is 10.5mg/L, transparency is 26cm, flow velocity is 0.8m/s in step 3, and then flow velocity is down to 0.3m/s and hatches 8 days.
4. the method for Leusiscus brandti artificial breeding, enhancement releasing according to claim 1, is characterized in that:
Hatching 10 days in the hatchery that water temperature is 18 DEG C, the depth of water is 30cm, dissolved oxygen is more than 10.5mg/L, transparency is 28cm, flow velocity is 0.9m/s in step 3, and then flow velocity is down to 0.4m/s and hatches 10 days.
5. the method for Leusiscus brandti artificial breeding, enhancement releasing according to claim 1, is characterized in that: outer case inside, described hatchery is provided with incubation tank (7), and the number of incubation tank (7) is more than or equal to 4.
6. the method for Leusiscus brandti artificial breeding, enhancement releasing according to claim 1, is characterized in that: raun fish length 29cm, more than body weight 0.4kg in step one; Milter fish length 26cm, more than body weight 0.25kg, and extrusion flow seminal fluid.
7. the method for Leusiscus brandti artificial breeding, enhancement releasing according to claim 1, is characterized in that: store up in step one in cage and store up density and be 40 Wei/㎡.
CN201410418653.1A 2014-08-22 2014-08-22 The method of Leusiscus brandti artificial breeding, enhancement releasing Expired - Fee Related CN104145866B (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105123592B (en) * 2015-07-23 2017-09-22 吴娇宇 A kind of bait feeding system of body length based on Micropterus salmoides
CN106804487B (en) * 2016-12-27 2022-05-17 珠江水利委员会珠江水利科学研究院 Testing device and method for researching movement mechanism of roe
CN106719170B (en) * 2016-12-30 2020-08-11 贵州锦润水产品有限责任公司 Sturgeon hatching method
CN106879515A (en) * 2017-04-12 2017-06-23 布尔津县额河特有冷水鱼繁育开发有限公司 A kind of viscidity-removing method of body graining embryonated egg high
CN108739542B (en) * 2018-06-15 2021-06-08 中国水产科学研究院黑龙江水产研究所 Artificial propagation method for three fish of globefish
CN109644904A (en) * 2019-01-30 2019-04-19 中国水产科学研究院黑龙江水产研究所 One kind is in spawning ground river salmon-type three seasons enhancement releasing method
CN111990294A (en) * 2019-12-31 2020-11-27 新疆维吾尔自治区水产科学研究所 Original seed harvesting, domesticating and artificial propagation method of Aschersonia songorica
CN112262792A (en) * 2020-09-10 2021-01-26 中国水产科学研究院黑龙江水产研究所 Large-scale zander hatching method
CN113261517B (en) * 2021-05-31 2022-05-31 中国水产科学研究院黑龙江水产研究所 Bacteriostatic debonding agent, preparation method thereof and method for improving artificial breeding hatchability of Atalantic malabaricus via bacteriostatic debonding agent

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101703022A (en) * 2009-11-27 2010-05-12 史建全 Artificial propagation and releasing technology of Gymnocypris przewalskii
CN201640193U (en) * 2010-03-31 2010-11-24 来长青 Simple sturgeon oosperm incubation device
CN201839704U (en) * 2010-11-22 2011-05-25 中国水产科学研究院长江水产研究所 Device for hatching and cultivating fish seed
CN203072660U (en) * 2013-03-12 2013-07-24 中国水产科学研究院黑龙江水产研究所 Incubation and cultivation device for incubating roe and culturing fry of salmon trout
CN203219813U (en) * 2013-04-27 2013-10-02 上海市松江区水产良种场 Songjiang perch hatching device

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060196440A1 (en) * 2005-03-05 2006-09-07 Oceanboy Farms, Inc. Hatchery system and method
KR101334604B1 (en) * 2011-11-30 2013-11-29 군산대학교산학협력단 An incubation bath with artificial spawning bed for fish and fish incubation method

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101703022A (en) * 2009-11-27 2010-05-12 史建全 Artificial propagation and releasing technology of Gymnocypris przewalskii
CN201640193U (en) * 2010-03-31 2010-11-24 来长青 Simple sturgeon oosperm incubation device
CN201839704U (en) * 2010-11-22 2011-05-25 中国水产科学研究院长江水产研究所 Device for hatching and cultivating fish seed
CN203072660U (en) * 2013-03-12 2013-07-24 中国水产科学研究院黑龙江水产研究所 Incubation and cultivation device for incubating roe and culturing fry of salmon trout
CN203219813U (en) * 2013-04-27 2013-10-02 上海市松江区水产良种场 Songjiang perch hatching device

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
2006年度绥芬河大麻哈鱼人工繁殖与孵化放流;赵春刚等;《水产学杂志》;20061130;第19卷(第2期);第54-57页 *
勃氏雅罗鱼苗种繁育及成鱼养殖技术;杨荣国等;《河北渔业》;20120120(第1期);第33-356页 *
绥芬河滩头雅罗鱼孵化放流的初步试验;董崇智等;《淡水渔业》;19910302(第2期);第6-8页 *
绥芬河滩头雅罗鱼的人工放流;董崇智等;《黑龙江水产》;19970815(第3期);第9-10页 *

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