CN106879515A - A kind of viscidity-removing method of body graining embryonated egg high - Google Patents
A kind of viscidity-removing method of body graining embryonated egg high Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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Abstract
The present invention is a kind of viscidity-removing method of body graining embryonated egg high, and in particular to the technical field of the viscous fertilized ovum unsticking of fish.The method is comprised the following steps：It is 1 according to mass ratio by embryonated egg mixed liquor and clay mixed liquor:13 mixing, stir 57 minutes, obtain unsticking mixed liquor, refilter unsticking mixed liquor, obtain the embryonated egg after unsticking, are washed with water the embryonated egg after clean unsticking, obtain embryonated egg；Wherein, the embryonated egg mixed liquor is：The fish-egg of body graining high and seminal fluid are mixed, is stirred 0.5 1.5 minutes, added physiological saline and stir 0.5 1.5 minutes, obtain embryonated egg mixed liquor.A kind of viscidity-removing method of body graining embryonated egg high of the present invention, unsticking is carried out using clay mixed liquor, and method is simple, and low cost, unsticking effect is good, can greatly improve embryonated egg survival rate.
Description
Technical field
The present invention relates to a kind of viscidity-removing method of body graining embryonated egg high.
Background technology
Body graining (Leuciscus baicalensis) high belongs to Cypriniformes, Cyprinidae, Leuciscinae, leuciscus.Again
Name ide.The sub- cold water fish of category.Perch in the more slow rivers of current and lake more.Xinjiang volume is detected in China
Er Qisi River systems, have been put into Xinjiang second class protection fish.The morphological feature of body graining high is：Body is long, flat-sided, abdomen circle,
Without abdomen rib, body is quite high, high 3.2-3.7 times of a length of body of body, and afterbody is more elongated；Kiss end circle is blunt, mouth end position, in the shape of a hoof, up and down
Jaw is about isometric.Eye is medium big；Completely, front end is micro-bend to abdomen side for side line；Squama is medium big, and lateral line scales are 56-61, and abdomeinal fin base portion is each
There is an elongated armpit squama；Body back is in grey black, and squama periphery black, belly is white, and pectoral fin, abdomeinal fin and anal fin are light yellow.Juvenile fish with
Zooplankter is food, and adult fish is then more to be dwelt aquatic insect as main foodstuff the bottom of with, and also eat small fish sometimes.Age at sexual maturity is generally 3-
5 ages, the egg-laying season, at the beginning of 6 months, originates in the waters of bottom sandstone and water plant more by the end of April ovum.Common individual body 23.5- long
36.0 centimetres, 288-948 grams of body weight is maximum individual up to 60 centimetres, weighs 4 kilograms or so.
Body graining high is important fishery object in the Siberia of Russia, is also the right of local pond culture
As being also the Important Economic fish of Xinjiang of China Burqin.In protection as early as possible and the local cold water fish resource of exploitation, accelerate me
The reasonable development paces of the peculiar economic fish in area, make great efforts open Europe and Central Asia market, cultivate leading enterprise so as to promote Xinjiang
In fishery industrialization development process, fry supply amount and quality, also large size fingerling Cultivating techniques, as greatly developing
With the key issue promoted during body graining high is propagated artificially.Because body graining embryonated egg high is viscous fertilized ovum, deposit
In the phenomenon of the easy adhesion of embryonated egg, so as to cause embryonated egg water mold long, anoxic and it is dead, fry hatching survival rate is relatively low, it is impossible to
Meet the market demand well.If separating it with the soft embryonated egg of hand, but easily rub and split embryonated egg, cause embryonated egg dead
Die, therefore the embryonated egg of body graining high needs unsticking.
Current unsticking generally uses talcum powder solution, and the embryonated egg of the granular states of 1-2 accounts for 45% or so after unsticking, under the state
Survival rate is only 40% or so, and remaining embryonated egg is sticked together, and there are one layer of white films on surface, airtight, makes embryonated egg
Ischaemia causes death.Therefore the problem of the easy adhesion of embryonated egg is not can solve.
In view of this, it is necessary to propose a kind of viscidity-removing method of new body graining embryonated egg high.
The content of the invention
It is an object of the invention to provide a kind of viscidity-removing method of body graining embryonated egg high, the method unsticking effect is good,
Embryonated egg survival rate can be greatly improved.
To achieve these goals, the technical solution adopted by the present invention is：
A kind of viscidity-removing method of body graining embryonated egg high, including：By embryonated egg mixed liquor and clay mixed liquor according to matter
Amount is than being 1:1-3 mixes, and stirs 5-7 minutes, obtains unsticking mixed liquor, refilters unsticking mixed liquor, obtains the embryonated egg after unsticking,
The embryonated egg after clean unsticking is washed with water, embryonated egg is obtained；
Wherein, the embryonated egg mixed liquor is：The fish-egg of body graining high and seminal fluid are mixed, are stirred 0.5-1.5 minutes,
Add physiological saline and stir 0.5-1.5 minutes, obtain embryonated egg mixed liquor.
Further, the embryonated egg mixed liquor and the mass ratio of clay mixed liquor are 1:2.
Further, the mass percent concentration of the clay mixed liquor is 15-25%.
Further, the mass percent concentration of the clay mixed liquor is 20%.
Further, the clay is yellow clay.
Further, the mixing time is 5-7 minutes.
Further, the filtering screen filtration of 40-60 mesh.
Further, the quality of the fish-egg and the volume ratio of seminal fluid are 1kg：1-2ml；
Fish-egg is 100 with the mass ratio of physiological saline:20-33.
Further, the mass percent concentration of the physiological saline is 0.6%-0.8%.
Compared with prior art, the beneficial effects of the present invention are：
1st, the viscidity-removing method of body graining embryonated egg high of the present invention, unsticking, clay are carried out using clay mixed liquor
Particle diameter be less than 2 μm, the particle diameter of talcum powder is 0.074mm-20.3 μm, and clay is smaller than the particle diameter of talcum powder, will not more increase
Plus the proportion of embryonated egg, the infringement to embryonated egg is smaller, can improve embryonated egg survival rate.
2nd, the viscidity-removing method of body graining embryonated egg high of the present invention, unsticking, clay are carried out using clay mixed liquor
Mixed liquor has preferable physical adsorbability and surface active, can play preferable unsticking effect, makes most fertilization
Ovum spreads out, so as to improve embryonated egg survival rate.
3rd, the viscidity-removing method of body graining embryonated egg high of the present invention, unsticking, method are carried out using clay mixed liquor
Simplicity, low cost can also greatly improve embryonated egg survival rate.
Specific embodiment
In order to a kind of viscidity-removing method of body graining embryonated egg high of the present invention is expanded on further, expected goal of the invention is reached,
Below in conjunction with preferred embodiment, to according to a kind of viscidity-removing method of body graining embryonated egg high proposed by the present invention, its specific reality
Mode, structure, feature and effect are applied, is described in detail as after.In the following description, different " embodiment " or " embodiment " refers to
Be not necessarily same embodiment.Additionally, the special characteristic, structure or feature in one or more embodiments can be by any suitable shapes
Formula is combined.
Before a kind of viscidity-removing method of body graining embryonated egg high of the present invention is elaborated, it is necessary to being carried in the present invention
And noun and method be described further, to reach more preferable effect.
Parent population, refers to development to sexal maturity stage, there is the milter or raun of fertility, also cries kind of a fish.
Viscous fertilized ovum, the proportion of embryonated egg has goo more than water embryonated egg egg membrane outer layer, can be sticked to after output
On the objects such as pasture and water, without sinking under water.The fish-egg adhesive of body graining high is not strong, after as embryonated egg, with very strong
Adhesive.
Clay be particle it is very small (<2 μm) plastic aluminium silicate salt is by long-term by rocks containing feldspar in the earth's crust
Weathering and the effect of geology and generate, be divided into nature extensively, species is various, clay has that particle is thin, plasticity is strong,
The advantages of associativity is good.Clay has plasticity after being moistened with water, and specific surface area is big, and elecrtonegativity is carried on particle, therefore
There are good physical adsorbability and chemical mobility of the surface, with the ability with other cation exchanges, be very easy to and water, mineral
Matter and organic nutrient substance are combined.Clay has ultralight clay, paper clay, yellow clay etc., and currently preferred is yellow clay.
What the present invention was used is body cavity injection, and injection site is pectoral fin base portion.During injection, first fish head forward, belly to
On set, then syringe pointed to the direction of head, and fish body into 45-60 degree angle, in pectoral fin base portion, the recess without squama is pierced into.
The depth that syringe needle is pierced into fish body should be grasped according to fish body size during injection, it is to avoid too deep to injure internal organ and bone, should also avoided
Shallow, liquid reflux is external.
Luteinizing hormone releasing hormone, also known as LHRH-A2, it is oxytocic drug, luteinizing hormone releasing hormone
It is a kind of peptide matters of hypothalamus secretion, acts on hypophysis and produce promoting sexual gland hormone (LH), also referred to as gonadotropin releasing hormone
Hormone, the regulation and control to vertebrate germ play an important role.
Maleic acid DOM, also known as DOM, are pimozide, can block dopamine and promoting sexual gland hormone (GTH) is discharged
Inhibitory action, promote GTH release；It is adjuvant of hastening parturition, is not used alone typically, coordinates to improve with other oxytocic and hasten parturition
Rate.
Maleic acid DOM (DOM) and luteinizing hormone releasing hormone (LHRH-A2) be used in combination, under can completely replacing fish brain
Hypophysis, can preferably promote gonadal hormone to discharge and ovulate, and be mainly used in the artificial propagation of fish.
Dry method of artificial fertilization is one kind of Technique in Fishes insemination, during the ovum and seminal fluid of parent population are clamp-oned without water receptacle, makes it
Add water a kind of artificial insemination method of rinsing after contact.
Lotic hatching：The method for making hatch fish roe in the device for have flowing water.Water is allowed slowly to move up, by water body
Exchange, keep water cleaning.
After the method referred in having understood the present invention, below in conjunction with specific embodiments, to of the invention a kind of high
The viscidity-removing method of body graining embryonated egg is further described in detail：
Embodiment 1.
Concrete operation step is as follows
(1) parent population is selected：Parent is carried out to body graining high to fish for, parent is judged from the surface of fish body and with hand touch
Piscinity gland Developmental maturity；The good female parent population of gonad development, belly is soft to be expanded, and substantially, gonopore is red prominent, body for ovary profile
Both sides are smooth, and there is harsh feeling ripe milter, head and body both sides, and head has pearl star, and light pressure belly has white seminal fluid to flow out,
Seminal fluid is met water and is scattered；The parent population for choosing according to male and female quantity ratio be 1:2 or 1：3 carry out combo, are placed in spawning pond, prepare people
Work is hastened parturition；Spawning pond is cement pit, and 1 meter of the depth of water, area is 30 square metres, and water temperature is 18 DEG C, and 4 spawning ponds place 25 respectively
Group, 27 groups, 30 groups and 33 groups of parent populations, it is 75,84,93 and 101 that parent population quantity is placed respectively.Every quality of parent population
In 1kg or so, the lighter parent population of body weight is 1 according to male and female quantity ratio:3 combos.
(2) artificial induced spawning：By the luteinizing hormone releasing hormone of 0.5mg and the maleic acid DOM of 0.2g, add
Mass percent concentration to 100ml is 0.7% physiological saline, and stirring obtains oxytocic drug to dissolving；Oxytocic drug is noted
Every pectoral fin base portion of parent population is mapped to, every 1 kg body weight of raun injects the oxytocic drug of 1ml, the every 2 kg body weight injection of milter
The oxytocic drug of 1ml.Oxytocic drug is now with the current.
(3) artificial insemination：After having spent 12 hours, a situation for parent population was checked every 2 hours, when pool wall is shown in ovum, raun
Gonopore benefit ovum, spawning peak starts, and parent population is caught immediately；White ceramic bowl is dried, the parent population that then will have been caught with towel
Dry, during fish-egg clamp-oned into basin, and clamp-on the seminal fluid of milter at once, the fish-egg per 1kg adds the seminal fluid of 1-2ml, quick stirring
0.5 minute, the mass percent concentration for adding 300g was 0.7% physiological saline, is quickly stirred with drake feather 1 minute, is obtained
Embryonated egg mixed liquor.Physiological saline is added to be conducive to insemination；Milter only removes a seminal fluid, because secondary semen quality is not so good as
The seminal fluid of first time.
The parent population that fish-egg and seminal fluid will be taken is placed in postpartum culture pond, supplements bait, recovers parent's constitution.
(4) unsticking：Mixed to the yellow clay that mass percent concentration is 20% is added in well mixed embryonated egg mixed liquor
Liquid is closed, the embryonated egg mixed liquor per 1kg adds the yellow clay mixed liquor of 2kg, and is stirred 7 minutes with drake feather, obtains unsticking mixed liquor,
Again with the screen filtration unsticking mixed liquor of 50 mesh, the embryonated egg after unsticking is obtained, then the fertilization after clean unsticking is eluriated with clear water
Ovum, obtains embryonated egg.
(5) artificial incubation：Embryonated egg is respectively put into the hatching barrel equipped with 250kg water, 48 are placed in each hatching barrel
Ten thousand -51 ten thousand embryonated eggs (i.e. the embryonated egg of 3kg or so), carry out Lotic hatching, and hatching water temperature is 18 DEG C, and dissolved oxygen amount is 8mg/L,
Start to emerge successively after 10 days；When fry can arbitrarily move about, carry out opening and feed.
Be contrast experiment simultaneously, be control group 1, unsticking use mass percent concentration for 20% talcum powder solution,
Unsticking 5 minutes, other operations are identical.
Table 1
As it can be seen from table 1 using talcum powder solution unsticking in control group 1, the granular states of embryonated egg 1-2 are 45%, this state
Lower embryonated egg survival rate only up to 40%, remaining overwhelming majority embryonated egg is sticked together, and there are one layer of white films on surface, airtight,
Cause embryonated egg ischaemia dead, embryonated egg survival rate is relatively low；The present embodiment clay mixed liquor unsticking, embryonated egg 1-2
State is 85%, and embryonated egg survival rate is up to 80% under this state.Can be drawn through comparing, using clay mixed liquor unsticking effect
It is good, embryonated egg survival rate can be significantly improved, increase fry quantity delivered.
The viscidity-removing method of the body graining embryonated egg a kind of high described in the present embodiment, unsticking is carried out using clay mixed liquor,
Method simplicity effect is good, and cost is not high；Clay mixed liquor has preferable physical adsorbability and surface active, the particle diameter of clay
<2 μm, the particle diameter than talcum powder is smaller, will not increase the proportion of embryonated egg, and the infringement to embryonated egg is smaller, and unsticking effect is more preferable,
Embryonated egg adhesion phenomenon can be reduced, embryonated egg survival rate is greatly improved, increases fry quantity delivered, better meeting market needs
Ask, the market of open body graining high.
Embodiment 2.
Concrete operation step is as follows
(1) parent population is selected：Parent is carried out to body graining high to fish for, parent is judged from the surface of fish body and with hand touch
Piscinity gland Developmental maturity；The good female parent population of gonad development, belly is soft to be expanded, and substantially, gonopore is red prominent, body for ovary profile
Both sides are smooth, and there is harsh feeling ripe milter, head and body both sides, and head has pearl star, and light pressure belly has white seminal fluid to flow out,
Seminal fluid is met water and is scattered；The parent population for choosing according to male and female quantity ratio be 1:2 or 1：3 carry out combo, are placed in spawning pond, prepare people
Work is hastened parturition；Spawning pond is cement pit, and 1 meter of the depth of water, area is 30 square metres, and water temperature is 15 DEG C, and 4 spawning ponds place 25 respectively
Group, 28 groups, 29 groups and 30 groups of parent populations, it is 76,82,86 and 100 that parent population quantity is placed respectively.Every quality of parent population
In 1kg or so, the lighter parent population of body weight is 1 according to male and female quantity ratio:3 combos.
(2) artificial induced spawning：By the luteinizing hormone releasing hormone of 0.4mg and the maleic acid DOM of 0.2g, add
Mass percent concentration to 200ml is 0.6% physiological saline, and stirring obtains oxytocic drug to dissolving；Oxytocic drug is noted
Every pectoral fin base portion of parent population is mapped to, every 1 kg body weight of raun injects the oxytocic drug of 1ml, the every 2 kg body weight injection of milter
The oxytocic drug of 1ml.Oxytocic drug is now with the current.
(3) artificial insemination：After having spent 12 hours, a situation for parent population was checked every 2 hours, when pool wall is shown in ovum, raun
Gonopore benefit ovum, spawning peak starts, and parent population is caught immediately；White ceramic bowl is dried, the parent population that then will have been caught with towel
Dry, during fish-egg clamp-oned into basin, and clamp-on the seminal fluid of milter at once, the fish-egg per 1kg adds the seminal fluid of 1-2ml, quickly stirs 1
Minute, the mass percent concentration for adding 200g is 0.6% physiological saline, is quickly stirred with drake feather 0.5 minute, is received
Smart ovum mixed liquor.Physiological saline is added to be conducive to insemination；Milter only removes a seminal fluid, because secondary semen quality is not as the
Seminal fluid once.
(4) unsticking：Mixed to the yellow clay that mass percent concentration is 15% is added in well mixed embryonated egg mixed liquor
Liquid is closed, the embryonated egg mixed liquor per 1kg adds the yellow clay mixed liquor of 3kg, and is stirred 5 minutes with drake feather, obtains unsticking mixed liquor,
Again with the screen filtration unsticking mixed liquor of 40 mesh, the embryonated egg after unsticking is obtained, then with the embryonated egg after clear water wash clean unsticking,
Obtain embryonated egg.
(5) artificial incubation：Embryonated egg is respectively put into the hatching barrel equipped with 250kg water, 48 are placed in each hatching barrel
Ten thousand -51 ten thousand embryonated eggs (i.e. the embryonated egg of 3kg or so), carry out Lotic hatching, and hatching water temperature is 17 DEG C, and dissolved oxygen amount is 7mg/L,
Start to emerge successively after 10 days；When fry can arbitrarily move about, carry out opening and feed.
Be contrast experiment simultaneously, be control group 2, unsticking use mass percent concentration for 15% talcum powder solution,
Unsticking 5 minutes, other operations are identical.
Table 2
Know from table 2, talcum powder solution unsticking is used in control group 2, and the granular states of embryonated egg 1-2 are 46%, are fertilized under this state
Ovum survival rate only up to 39%, remaining overwhelming majority embryonated egg is sticked together, and there are one layer of white films on surface, airtight, causes to receive
Smart ovum ischaemia is dead, and embryonated egg survival rate is relatively low；The present embodiment clay mixed liquor unsticking, the granular states of embryonated egg 1-2 are
81%, embryonated egg survival rate can reach 82% under this state.Can be drawn through comparing, using clay mixed liquor unsticking effect
It is good, embryonated egg survival rate can be significantly improved, increase fry quantity delivered.
The viscidity-removing method of the body graining embryonated egg a kind of high described in the present embodiment, unsticking is carried out using clay mixed liquor,
Method simplicity effect is good, and cost is not high；Clay mixed liquor has preferable physical adsorbability and surface active, the particle diameter of clay
<2 μm, the particle diameter than talcum powder is smaller, will not increase the proportion of embryonated egg, and the infringement to embryonated egg is smaller, and unsticking effect is more preferable,
Embryonated egg adhesion phenomenon can be reduced, embryonated egg survival rate is greatly improved, increases fry quantity delivered, better meeting market needs
Ask, the market of open body graining high.
Embodiment 3.
Concrete operation step is as follows
(1) parent population is selected：Parent is carried out to body graining high to fish for, parent is judged from the surface of fish body and with hand touch
Piscinity gland Developmental maturity；The good female parent population of gonad development, belly is soft to be expanded, and substantially, gonopore is red prominent, body for ovary profile
Both sides are smooth, and there is harsh feeling ripe milter, head and body both sides, and head has pearl star, and light pressure belly has white seminal fluid to flow out,
Seminal fluid is met water and is scattered；The parent population for choosing according to male and female quantity ratio be 1:2 or 1：3 carry out combo, are placed in spawning pond, prepare people
Work is hastened parturition；Spawning pond is cement pit, and 1 meter of the depth of water, area is 30 square metres, and water temperature is 18 DEG C, and 4 spawning ponds place 25 respectively
Group, 29 groups, 32 groups and 33 groups of parent populations, it is 77,88,94 and 101 that parent population quantity is placed respectively.Every quality of parent population
In 1kg or so, the lighter parent population of body weight is 1 according to male and female quantity ratio:3 combos.
(2) artificial induced spawning：By the luteinizing hormone releasing hormone of 0.5mg and the maleic acid DOM of 0.3g, add
Mass percent concentration to 100ml is 0.8% physiological saline, and stirring obtains oxytocic drug to dissolving；Oxytocic drug is noted
Every pectoral fin base portion of parent population is mapped to, every 1 kg body weight of raun injects the oxytocic drug of 1ml, the every 2 kg body weight injection of milter
The oxytocic drug of 1ml.Oxytocic drug is now with the current.
(3) artificial insemination：After having spent 12 hours, a situation for parent population was checked every 3 hours, when pool wall is shown in ovum, raun
Gonopore benefit ovum, spawning peak starts, and parent population is caught immediately；White ceramic bowl is dried, the parent population that then will have been caught with towel
Dry, during fish-egg clamp-oned into basin, during fish-egg clamp-oned into basin, and clamp-on the seminal fluid of milter at once, the fish-egg per 1kg adds 1-2ml
Seminal fluid, quick stirring 1.5 minutes, the mass percent concentration for adding 330g is 0.8% physiological saline, quick with drake feather
Stirring 1.5 minutes, obtains embryonated egg mixed liquor.Physiological saline is added to be conducive to insemination；Milter only removes a seminal fluid, because second
Secondary semen quality is not as the seminal fluid of first time.
The parent population that fish-egg and seminal fluid will be taken is placed in postpartum culture pond, supplements bait, recovers parent's constitution.
(4) unsticking：Mixed to the yellow clay that mass percent concentration is 25% is added in well mixed embryonated egg mixed liquor
Liquid is closed, the embryonated egg mixed liquor per 1kg adds the yellow clay mixed liquor of 1kg, and is stirred 6 minutes with drake feather, obtains unsticking mixed liquor,
Again with the screen filtration unsticking mixed liquor of 60 mesh, the embryonated egg after unsticking is obtained, then the fertilization after clean unsticking is eluriated with clear water
Ovum, obtains embryonated egg.
(5) artificial incubation：Embryonated egg is respectively put into the hatching barrel equipped with 250kg water, 48 are placed in each hatching barrel
Ten thousand -51 ten thousand embryonated eggs (i.e. the embryonated egg of 3kg or so), carry out Lotic hatching, and hatching water temperature is 16 DEG C, and dissolved oxygen amount is 6mg/L,
Start to emerge successively after 10 days；When fry can arbitrarily move about, carry out opening and feed.
Be contrast experiment simultaneously, be control group 3, unsticking use mass percent concentration for 25% talcum powder solution,
Unsticking 6 minutes, other operations are identical.
Table 3
From table 3 it can be seen that using talcum powder solution unsticking in control group, the granular states of embryonated egg 1-2 are 44%, under this state
Embryonated egg survival rate only up to 43%, remaining overwhelming majority embryonated egg is sticked together, and there are one layer of white films on surface, airtight, leads
Cause embryonated egg ischaemia dead, embryonated egg survival rate is relatively low；The present embodiment uses clay mixed liquor unsticking, embryonated egg 1-2
State is 83%, embryonated egg survival rate 81% under this state.Can be drawn through comparing, using clay mixed liquor unsticking effect
It is good, embryonated egg survival rate can be significantly improved, increase fry quantity delivered.
The viscidity-removing method of the body graining embryonated egg a kind of high described in the present embodiment, unsticking is carried out using clay mixed liquor,
Method simplicity effect is good, and cost is not high；Clay mixed liquor has preferable physical adsorbability and surface active, the particle diameter of clay
<2 μm, the particle diameter than talcum powder is smaller, will not increase the proportion of embryonated egg, and the infringement to embryonated egg is smaller, and unsticking effect is more preferable,
Embryonated egg adhesion phenomenon can be reduced, embryonated egg survival rate is greatly improved, increases fry quantity delivered, better meeting market needs
Ask, the market of open body graining high.
The above, is only the preferred embodiment of the embodiment of the present invention, and any shape is not made to the embodiment of the present invention
Limitation in formula, according to any simple modification, equivalent variations that the technical spirit of the embodiment of the present invention is made to above example
With modification, still fall within the range of embodiment of the present invention technical scheme.
Claims (9)
1. a kind of viscidity-removing method of body graining embryonated egg high, it is characterised in that including：Embryonated egg mixed liquor is mixed with clay
Liquid is 1 according to mass ratio:1-3 mixes, and stirs 5-7 minutes, obtains unsticking mixed liquor, unsticking mixed liquor is refiltered, after obtaining unsticking
Embryonated egg, be washed with water the embryonated egg after clean unsticking, obtain embryonated egg；
Wherein, the embryonated egg mixed liquor is：The fish-egg of body graining high and seminal fluid are mixed, is stirred 0.5-1.5 minutes, then added
Enter physiological saline and stir 0.5-1.5 minutes, obtain embryonated egg mixed liquor.
2. viscidity-removing method according to claim 1, it is characterised in that
The embryonated egg mixed liquor is 1 with the mass ratio of clay mixed liquor:2.
3. the viscidity-removing method according to claim 1 or claim 2, it is characterised in that
The mass percent concentration of the clay mixed liquor is 15%-25%.
4. viscidity-removing method according to claim 3, it is characterised in that
The mass percent concentration of the clay mixed liquor is 20%.
5. viscidity-removing method according to claim 4, it is characterised in that
The clay is yellow clay.
6. viscidity-removing method according to claim 1, it is characterised in that
The mixing time is 5-7 minutes.
7. viscidity-removing method according to claim 1, it is characterised in that
The filtering screen filtration of 40-60 mesh.
8. viscidity-removing method according to claim 1, it is characterised in that
The quality of the fish-egg is 1kg with the volume ratio of seminal fluid：1-2ml；
Fish-egg is 100 with the mass ratio of physiological saline:20-33.
9. viscidity-removing method according to claim 8, it is characterised in that
The mass percent concentration of the physiological saline is 0.6%-0.8%.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110050729A (en) * | 2019-05-23 | 2019-07-26 | 吉林省水产科学研究院 | A kind of Tumen graining artificial fecundation method |
| CN111296333A (en) * | 2020-03-30 | 2020-06-19 | 辽宁省淡水水产科学研究院 | Crossbreeding method for female high-body elegans and male walnuts |
| CN115316315A (en) * | 2022-08-23 | 2022-11-11 | 北京市水生野生动植物救护中心 | Secondary debonding method and application of fertilized eggs of Atalanobis walskii |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101933477A (en) * | 2010-08-12 | 2011-01-05 | 常熟理工学院 | Artificial propagation method of Odontobutis obscura |
| CN102301972A (en) * | 2011-08-05 | 2012-01-04 | 句容市句容水库管理所 | Method for artificially breeding Plagiognathops microlepis |
| CN103329833A (en) * | 2013-07-23 | 2013-10-02 | 陈道印 | Cross breeding method for Japanese crucian carp and cyprinus carpio red |
| CN103348937A (en) * | 2013-06-17 | 2013-10-16 | 句容市句容水库管理所 | Artificial propagation method for squaliobarbus curriculus |
| CN104145866A (en) * | 2014-08-22 | 2014-11-19 | 王维坤 | Method for artificially breeding, reproducing and releasing dace (leuciscus brandti) |
| CN105230537A (en) * | 2015-10-14 | 2016-01-13 | 南宁学院 | Artificial insemination method for mystus guttatus |
-
2017
- 2017-04-12 CN CN201710236909.0A patent/CN106879515A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101933477A (en) * | 2010-08-12 | 2011-01-05 | 常熟理工学院 | Artificial propagation method of Odontobutis obscura |
| CN102301972A (en) * | 2011-08-05 | 2012-01-04 | 句容市句容水库管理所 | Method for artificially breeding Plagiognathops microlepis |
| CN103348937A (en) * | 2013-06-17 | 2013-10-16 | 句容市句容水库管理所 | Artificial propagation method for squaliobarbus curriculus |
| CN103329833A (en) * | 2013-07-23 | 2013-10-02 | 陈道印 | Cross breeding method for Japanese crucian carp and cyprinus carpio red |
| CN104145866A (en) * | 2014-08-22 | 2014-11-19 | 王维坤 | Method for artificially breeding, reproducing and releasing dace (leuciscus brandti) |
| CN105230537A (en) * | 2015-10-14 | 2016-01-13 | 南宁学院 | Artificial insemination method for mystus guttatus |
Non-Patent Citations (1)
| Title |
|---|
| 蔡仁逵: "《淡水养鱼手册》", 28 February 1987, 上海科学技术出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110050729A (en) * | 2019-05-23 | 2019-07-26 | 吉林省水产科学研究院 | A kind of Tumen graining artificial fecundation method |
| CN111296333A (en) * | 2020-03-30 | 2020-06-19 | 辽宁省淡水水产科学研究院 | Crossbreeding method for female high-body elegans and male walnuts |
| CN115316315A (en) * | 2022-08-23 | 2022-11-11 | 北京市水生野生动植物救护中心 | Secondary debonding method and application of fertilized eggs of Atalanobis walskii |
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