CN105230537A - Artificial insemination method for mystus guttatus - Google Patents
Artificial insemination method for mystus guttatus Download PDFInfo
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- CN105230537A CN105230537A CN201510660298.3A CN201510660298A CN105230537A CN 105230537 A CN105230537 A CN 105230537A CN 201510660298 A CN201510660298 A CN 201510660298A CN 105230537 A CN105230537 A CN 105230537A
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- guttatus
- artificial insemination
- seminal fluid
- fertilized egg
- insemination method
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- 238000000034 method Methods 0.000 title claims abstract description 25
- 230000009027 insemination Effects 0.000 title claims abstract description 23
- 241000746773 Hemibagrus guttatus Species 0.000 title abstract 4
- 210000000582 semen Anatomy 0.000 claims abstract description 35
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 18
- 230000012447 hatching Effects 0.000 claims abstract description 16
- 239000000203 mixture Substances 0.000 claims abstract description 16
- 229910052573 porcelain Inorganic materials 0.000 claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 239000004677 Nylon Substances 0.000 claims abstract description 10
- 229920001778 nylon Polymers 0.000 claims abstract description 10
- 238000003756 stirring Methods 0.000 claims abstract description 9
- 102000002322 Egg Proteins Human genes 0.000 claims description 21
- 108010000912 Egg Proteins Proteins 0.000 claims description 21
- 210000004681 ovum Anatomy 0.000 claims description 21
- 239000002504 physiological saline solution Substances 0.000 claims description 15
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 8
- 229910001570 bauxite Inorganic materials 0.000 claims description 8
- 239000000843 powder Substances 0.000 claims description 8
- 239000011780 sodium chloride Substances 0.000 claims description 8
- 230000004720 fertilization Effects 0.000 abstract description 9
- 241000251539 Vertebrata <Metazoa> Species 0.000 abstract description 2
- 238000001914 filtration Methods 0.000 abstract 2
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 238000000227 grinding Methods 0.000 abstract 1
- 238000002156 mixing Methods 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
- 239000010984 cultured pearl Substances 0.000 description 8
- 230000013020 embryo development Effects 0.000 description 8
- 238000011534 incubation Methods 0.000 description 3
- 210000001161 mammalian embryo Anatomy 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 210000002808 connective tissue Anatomy 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 230000032696 parturition Effects 0.000 description 2
- 206010003497 Asphyxia Diseases 0.000 description 1
- 241000555268 Dendroides Species 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 230000026109 gonad development Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention relates to the technical field of cultivation of vertebrate, and particularly to an artificial insemination method for mystus guttatus. The method comprises taking spermarys of the male mystus guttatus, cutting the spermarys into pieces, grinding the spermarys into a mushy state, extruding the mushy spermarys through a 100 to 150 mesh nylon bolting-silk tulle to obtain a seminal fluid; uniformly mixing the seminal fluid and normal saline whose concentration is within the range from 0.67 % to 0.70 %, and putting the above mixture into a porcelain basin, stirring the above mixture and spawns for 4 to 8 min at the temperature of 15 to 20 DEG C to obtain oosperms; adding water into the porcelain basin, standing for 28 to 35 min, filtering to obtaining oosperms whose diameters are larger than or equal to 3mm; and stirring the oosperms whose diameters are larger than or equal to 3mm and a debond agent for 5 to 10 min, filtering, washing for 3 to 5 times to obtain hatching oosperms. By adopting the artificial insemination method for mystus guttatus, the rate of fertilization is high, the quality of oosperms is good, the hatching cost can be saved, and the hatching efficiency can be raised.
Description
Technical field
The present invention relates to vertebrate cultural technique, be specifically related to the artificial insemination method of a kind of Ban Guttatus.
Background technology
Spot Of-digestive-tract is the species that Chang section Of-digestive-tract belongs to, enjoy the good reputation of " king of fresh water " and the Zhujiang River " four your name fishes ", its fine and tender taste, delicious, is of high nutritive value, deeply like by consumers in general and culturist, there is wide promotion prospect, but its rare numbers, can not market demand be met, therefore, technology of artificial insemination Weis that the batch production of Ban Guttatus provides a new approach.
In the prior art, as the growth of document 《 Ban Guttatus, breeding, feeding habits and cultural technique " (Li Hongjing, Chu Zhangjie, hubei agricultural science, 2006, 45 (6): 807-809), " spot Guttatus artificial induced spawning technical essential " (Li Junmin, Li Yan, science is breeded fish, 1998 (6): 21-22), the Primary Study of 《 Ban Guttatus artificial propagation " (circuit, Zhou Tao, Yang Kai etc., Guangdong Ocean University's journal, 2008, 28 (4): 83-84), " Primary Study of the spot Guttatus artificial propagation of cultured pearls river, pond and embryonic development " (Zhu Xinping, Chen Kun is kind, Xie Gang etc., Dalian aquatic product Academy journal, 2005, 20 (4): 352-354), equal Dui Ban Guttatus has carried out profound research, the development of Dan Ban Guttatus technology of artificial insemination is shorter, still there is following problems: ① Ban Guttatus milter spermary is dendroid, be difficult to extrude seminal fluid, and then increase test-tube cost, 2., when extracting seminal fluid, the connective tissue in seminal fluid easily adheres on fish-egg, and meanwhile, fish-egg belongs to viscid egg, therefore when sperm ovum binding, adhesion is excessive, easily causes fertilized egg death by suffocation, reduces the incubation rate of fertilized egg, and then affects Ban Guttatus output.
Summary of the invention
The object of the present invention is to provide the artificial insemination method of a kind of Ban Guttatus, becoming pieces of an egg for solving Ban Guttatus parent population fertilized egg in conjunction with insufficient and viscous fertilized ovum chance water condensation, causing fertilization rate, incubation rate low, and then affecting the problem of its breeding production.
Be achieved especially by following scheme:
An artificial insemination method of Ban Guttatus, is characterized in that, comprises the following steps:
(1) dissect Ban Guttatus milter, take out spermary, shredded, being ground to spermary is rotten shape, is used 100-150 order nylon mesh tulle to extrude, and obtains seminal fluid;
(2) after the physiological saline being 0.67-0.70% by seminal fluid and concentration mixes, proceed to porcelain basin, at temperature is 15-20 DEG C, after fish-egg mix and blend 4-8min, obtain fertilized egg;
(3) add water in porcelain basin, leave standstill 28-35min, filter, obtain the fertilized egg of ovum footpath >=3mm;
(4) be in 18-23 DEG C in temperature, after the fertilized egg of ovum footpath >=3mm and detackifier are stirred 5-10min, filter, wash 3-5 time, obtain and treat hatching fertilized egg.
Described nylon mesh tulle is 120 orders.
The volume ratio of described seminal fluid and physiological saline is 1:10-15.
The volume ratio of described seminal fluid and fish-egg is 1:100-120.
The volume ratio of described fertilized egg and detackifier is 1-1.5:10.
Described detackifier is by weight for 3-3.5:0.75-1:1:40 mixes by diatomite, bauxite powder, sodium chloride, water.
Described stirring, its rotating speed is 30-50r/min.
Beneficial effect of the present invention
The present invention adopts and first dissects, grind spermary again to rotten shape, be conducive to extruding of seminal fluid, and ensure the natural rate of interest of sperm, then bolting silk net extruding spermary material is used, while fully extracting milter seminal fluid, the connective tissue avoiding spermary sticks on egg cell, is conducive to smart ovum and fully combines, and in conjunction with the use of physiological saline, promote gonad development, form larger spermary wall, and then improve fertilization rate; Process of Insemination temperature is controlled at 15-20 DEG C simultaneously, ensure that smart ovum is survived and is in active state, be conducive to smart ovum and combine fast; Again in conjunction with the application of detackifier, diatomite, bauxite powder, sodium chloride are made detackifier by rational proportion, make viscous fertilized ovum unsticking equably, coordinate the concentration of detackifier, the control of serviceability temperature again, fertilized egg injury tolerance is down to minimum, and then improve the incubation rate of fertilized egg, simultaneously, remove the impurity on fertilized egg surface, and then ensure that fineness and the transparency of fertilized egg ovum wall after unsticking, and then be easy to observe embryo's situation.After female Ban Guttatus being hastened parturition according to " Primary Study of pond cultured pearls Jiang Ban Guttatus artificial propagation and embryonic development " scheme by employing, the present invention program is adopted to carry out artificial insemination, and according to " Primary Study of pond cultured pearls Jiang Ban Guttatus artificial propagation and embryonic development " method, fertilized egg is hatched, result shows, the fertilization rate of the present invention program is higher, be approximately higher than the fertilization rate 1.2 times of " Primary Study of pond cultured pearls Jiang Ban Guttatus artificial propagation and embryonic development ", time of hatching is than " Primary Study of pond cultured pearls Jiang Ban Guttatus artificial propagation and embryonic development " few about 5h, illustrate: the present invention program's fertilization rate is high, Fertilized quality is good, be conducive to saving hatching cost and improving hatching efficiency.
Embodiment
Below in conjunction with concrete embodiment, further restriction is done to technical scheme of the present invention, but claimed scope is not only confined to done description.
Embodiment 1
An artificial insemination method of Ban Guttatus, is characterized in that, comprises the following steps:
(1) dissect Ban Guttatus milter, take out spermary, shredded, being ground to spermary is rotten shape, is used 120 order nylon mesh tulles to extrude, and obtains seminal fluid;
(2) after the physiological saline being 0.68% by seminal fluid and concentration mixes, proceed to porcelain basin, at temperature is 15 DEG C, after fish-egg mix and blend 5min, obtain fertilized egg;
(3) add water in porcelain basin, leave standstill 30min, filter, obtain the fertilized egg of ovum footpath >=3mm;
(4) be in 18-20 DEG C in temperature, after the fertilized egg of ovum footpath >=3mm and detackifier are stirred 8min, filter, wash 2 times, obtain and treat hatching fertilized egg.
The volume ratio of described seminal fluid and physiological saline is 1:15.
The volume ratio of described seminal fluid and fish-egg is 1:110.
The volume ratio of described fertilized egg and detackifier is 1:12.
Described detackifier is by weight for 3:1:1:40 mixes by diatomite, bauxite powder, sodium chloride, water.
Described stirring, its rotating speed is 45r/min.
Embodiment 2
An artificial insemination method of Ban Guttatus, is characterized in that, comprises the following steps:
(1) dissect Ban Guttatus milter, take out spermary, shredded, being ground to spermary is rotten shape, is used 100 order nylon mesh tulles to extrude, and obtains seminal fluid;
(2) after the physiological saline being 0.70% by seminal fluid and concentration mixes, proceed to porcelain basin, at temperature is 18 DEG C, after fish-egg mix and blend 4min, obtain fertilized egg;
(3) add water in porcelain basin, leave standstill 28min, filter, obtain the fertilized egg of ovum footpath >=3mm;
(4) be in 21-23 DEG C in temperature, after the fertilized egg of ovum footpath >=3mm and detackifier are stirred 5min, filter, wash 4 times, obtain and treat hatching fertilized egg.
The volume ratio of described seminal fluid and physiological saline is 1:12.
The volume ratio of described seminal fluid and fish-egg is 1:100.
The volume ratio of described fertilized egg and detackifier is 1:8.
Described detackifier is by weight for 3.2:0.8:1:40 mixes by diatomite, bauxite powder, sodium chloride, water.
Described stirring, its rotating speed is 42r/min.
Embodiment 3
An artificial insemination method of Ban Guttatus, is characterized in that, comprises the following steps:
(1) dissect Ban Guttatus milter, take out spermary, shredded, being ground to spermary is rotten shape, is used 150 order nylon mesh tulles to extrude, and obtains seminal fluid;
(2) after the physiological saline being 0.67% by seminal fluid and concentration mixes, proceed to porcelain basin, at temperature is 20 DEG C, after fish-egg mix and blend 8min, obtain fertilized egg;
(3) add water in porcelain basin, leave standstill 35min, filter, obtain the fertilized egg of ovum footpath >=3mm;
(4) be in 19-21 DEG C in temperature, after the fertilized egg of ovum footpath >=3mm and detackifier are stirred 10min, filter, wash 5 times, obtain and treat hatching fertilized egg.
The volume ratio of described seminal fluid and physiological saline is 1:10.
The volume ratio of described seminal fluid and fish-egg is 1:110.
The volume ratio of described fertilized egg and detackifier is 1.2:10.
Described detackifier is by weight for 3.5:1:1:40 mixes by diatomite, bauxite powder, sodium chloride, water.
Described stirring, its rotating speed is 50r/min.
Embodiment 4
An artificial insemination method of Ban Guttatus, is characterized in that, comprises the following steps:
(1) dissect Ban Guttatus milter, take out spermary, shredded, being ground to spermary is rotten shape, is used 120 order nylon mesh tulles to extrude, and obtains seminal fluid;
(2) after the physiological saline being 0.69% by seminal fluid and concentration mixes, proceed to porcelain basin, at temperature is 16 DEG C, after fish-egg mix and blend 4min, obtain fertilized egg;
(3) add water in porcelain basin, leave standstill 32min, filter, obtain the fertilized egg of ovum footpath >=3mm;
(4) be in 20-23 DEG C in temperature, after the fertilized egg of ovum footpath >=3mm and detackifier are stirred 7min, filter, wash 3 times, obtain and treat hatching fertilized egg.
The volume ratio of described seminal fluid and physiological saline is 1:10.
The volume ratio of described seminal fluid and fish-egg is 1:120.
The volume ratio of described fertilized egg and detackifier is 1:10.
Described detackifier is by weight for 3.5:0.9:1:40 mixes by diatomite, bauxite powder, sodium chloride, water.
Described stirring, its rotating speed is 39r/min.
Embodiment 5
An artificial insemination method of Ban Guttatus, is characterized in that, comprises the following steps:
(1) dissect Ban Guttatus milter, take out spermary, shredded, being ground to spermary is rotten shape, is used 150 order nylon mesh tulles to extrude, and obtains seminal fluid;
(2) after the physiological saline being 0.70% by seminal fluid and concentration mixes, proceed to porcelain basin, at temperature is 20 DEG C, after fish-egg mix and blend 6min, obtain fertilized egg;
(3) add water in porcelain basin, leave standstill 30min, filter, obtain the fertilized egg of ovum footpath >=3mm;
(4) be in 19-21 DEG C in temperature, after the fertilized egg of ovum footpath >=3mm and detackifier are stirred 10min, filter, clean 7 times, obtain and treat hatching fertilized egg.
The volume ratio of described seminal fluid and physiological saline is 1:10.
The volume ratio of described seminal fluid and fish-egg is 1:100.
The volume ratio of described fertilized egg and detackifier is 1.2:10.
Described detackifier is by weight for 3.5:1:1:40 mixes by diatomite, bauxite powder, sodium chloride, water.
Described stirring, its rotating speed is 44r/min.
Test example 1
1., after recording according to " Primary Study of pond cultured pearls Jiang Ban Guttatus artificial propagation and embryonic development " the female Ban Guttatus that hastens parturition, be fertilized by embodiment 1-5 scheme, and calculate fertilization rate, result is as shown in table 1:
Table 1
What 2. obtain embodiment 1-5 according to " Primary Study of pond cultured pearls Jiang Ban Guttatus artificial propagation and embryonic development " method treats that hatching fertilized egg is hatched, and the growth in record embryo's each stage is consuming time, as shown in table 2:
Table 2
As shown in Table 1, adopt the fertilization rate of embodiment 1-5 scheme higher, then associative list 2, the brooding time of the fertilized egg of the present embodiment is shorter, gastrul stage is entered through 14h30min, embryo hatching required time is about 50h, compares the record of " Primary Study of pond cultured pearls Jiang Ban Guttatus artificial propagation and embryonic development ", shortens the brooding time of nearly 5 hours, save hatching cost, illustrate that the Fertilized quality of the present invention program is more excellent, development condition is better, and fertilization rate is better.
Claims (7)
1. an artificial insemination method of spot Guttatus, is characterized in that, comprises the following steps:
(1) dissect Ban Guttatus milter, take out spermary, shredded, being ground to spermary is rotten shape, is used 100-150 order nylon mesh tulle to extrude, and obtains seminal fluid;
(2) after the physiological saline being 0.67-0.70% by seminal fluid and concentration mixes, proceed to porcelain basin, at temperature is 15-20 DEG C, after fish-egg mix and blend 4-8min, obtain fertilized egg;
(3) add water in porcelain basin, leave standstill 28-35min, filter, obtain the fertilized egg of ovum footpath >=3mm;
(4) be in 18-23 DEG C in temperature, after the fertilized egg of ovum footpath >=3mm and detackifier are stirred 5-10min, filter, wash 3-5 time, obtain and treat hatching fertilized egg.
2. the artificial insemination method of Ban Guttatus as claimed in claim 1, it is characterized in that, nylon mesh tulle is 120 orders.
3. the artificial insemination method of Ban Guttatus as claimed in claim 1, it is characterized in that, the volume ratio of described seminal fluid and physiological saline is 1:10.
4. the artificial insemination method of Ban Guttatus as claimed in claim 1, it is characterized in that, the volume ratio of described seminal fluid, mixed liquor of normal saline and fish-egg is 1:100-120.
5. the artificial insemination method of Ban Guttatus as claimed in claim 1, it is characterized in that, the volume ratio of described fertilized egg and detackifier is 1-1.5:10.
6. the artificial insemination method of Ban Guttatus as described in claim 1 or 6, it is characterized in that, described detackifier is by weight for 3-3.5:0.75-1:1:40 mixes by diatomite, bauxite powder, sodium chloride, water.
7. the artificial insemination method of Ban Guttatus as claimed in claim 1, it is characterized in that, described stirring, its rotating speed is 30-50r/min.
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| CN201510660298.3A CN105230537B (en) | 2015-10-14 | 2015-10-14 | A kind of Ban Guttatus artificial insemination method |
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| CN201510660298.3A CN105230537B (en) | 2015-10-14 | 2015-10-14 | A kind of Ban Guttatus artificial insemination method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106172146A (en) * | 2016-09-21 | 2016-12-07 | 南宁学院 | A kind of artificial insemination method of rainbow trout |
| CN106879515A (en) * | 2017-04-12 | 2017-06-23 | 布尔津县额河特有冷水鱼繁育开发有限公司 | A kind of viscidity-removing method of body graining embryonated egg high |
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| CN102613125A (en) * | 2012-04-26 | 2012-08-01 | 云南省渔业科学研究院 | Artificial insemination method of mystus nemurus |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106172146A (en) * | 2016-09-21 | 2016-12-07 | 南宁学院 | A kind of artificial insemination method of rainbow trout |
| CN106172146B (en) * | 2016-09-21 | 2019-05-14 | 南宁学院 | A kind of artificial insemination method of rainbow trout |
| CN106879515A (en) * | 2017-04-12 | 2017-06-23 | 布尔津县额河特有冷水鱼繁育开发有限公司 | A kind of viscidity-removing method of body graining embryonated egg high |
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Effective date of registration: 20221024 Address after: 902-b, building 5, 158 Xisan Road, Tianjin Binhai New Area pilot free trade zone (Airport Economic Zone) Patentee after: Tianjin Saiyi Automation Equipment Technology Co.,Ltd. Address before: 530200 No. 8, Ting Ting Road, Yongning District, Nanning, the Guangxi Zhuang Autonomous Region Patentee before: NANNING University |
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| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180313 |