CN105409873A - Method for controlling hoplobatrachus tigerinus sex ratio and application of method - Google Patents
Method for controlling hoplobatrachus tigerinus sex ratio and application of method Download PDFInfo
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- CN105409873A CN105409873A CN201510777824.4A CN201510777824A CN105409873A CN 105409873 A CN105409873 A CN 105409873A CN 201510777824 A CN201510777824 A CN 201510777824A CN 105409873 A CN105409873 A CN 105409873A
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- mercuric chloride
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- letrozole
- testosterone
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- 238000000034 method Methods 0.000 title claims abstract description 52
- 241001476406 Hoplobatrachus tigerinus Species 0.000 title abstract 4
- MUMGGOZAMZWBJJ-DYKIIFRCSA-N Testostosterone Chemical compound O=C1CC[C@]2(C)[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 MUMGGOZAMZWBJJ-DYKIIFRCSA-N 0.000 claims abstract description 58
- 229960003604 testosterone Drugs 0.000 claims abstract description 29
- 229960003881 letrozole Drugs 0.000 claims abstract description 28
- HPJKCIUCZWXJDR-UHFFFAOYSA-N letrozole Chemical compound C1=CC(C#N)=CC=C1C(N1N=CN=C1)C1=CC=C(C#N)C=C1 HPJKCIUCZWXJDR-UHFFFAOYSA-N 0.000 claims abstract description 28
- 230000029052 metamorphosis Effects 0.000 claims abstract description 11
- 229960002523 mercuric chloride Drugs 0.000 claims description 61
- LWJROJCJINYWOX-UHFFFAOYSA-L mercury dichloride Chemical compound Cl[Hg]Cl LWJROJCJINYWOX-UHFFFAOYSA-L 0.000 claims description 61
- 230000037396 body weight Effects 0.000 claims description 20
- 238000012258 culturing Methods 0.000 claims description 10
- 230000003203 everyday effect Effects 0.000 claims description 8
- 230000002354 daily effect Effects 0.000 claims description 7
- 230000000384 rearing effect Effects 0.000 claims description 2
- 230000008901 benefit Effects 0.000 abstract description 2
- 241000162910 Cacosternum Species 0.000 description 38
- 230000008569 process Effects 0.000 description 28
- 230000002159 abnormal effect Effects 0.000 description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 22
- 230000037213 diet Effects 0.000 description 8
- 235000005911 diet Nutrition 0.000 description 8
- 238000009395 breeding Methods 0.000 description 6
- 239000003814 drug Substances 0.000 description 6
- 230000001488 breeding effect Effects 0.000 description 5
- 229940079593 drug Drugs 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 210000000683 abdominal cavity Anatomy 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 210000002468 fat body Anatomy 0.000 description 2
- 230000012447 hatching Effects 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000003643 water by type Substances 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- VOXZDWNPVJITMN-ZBRFXRBCSA-N 17β-estradiol Chemical compound OC1=CC=C2[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 VOXZDWNPVJITMN-ZBRFXRBCSA-N 0.000 description 1
- 241000269350 Anura Species 0.000 description 1
- 241000132931 Batrachia Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 235000019750 Crude protein Nutrition 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 241000270934 Rana catesbeiana Species 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 239000003098 androgen Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000029602 competition with other organism Effects 0.000 description 1
- 235000019784 crude fat Nutrition 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 229960005309 estradiol Drugs 0.000 description 1
- 229930182833 estradiol Natural products 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000004459 forage Substances 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000000474 nursing effect Effects 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000003307 slaughter Methods 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
- Animal Behavior & Ethology (AREA)
- Marine Sciences & Fisheries (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a method for controlling hoplobatrachus tigerinus sex ratio and application of the method and belongs to the technical field of hoplobatrachus tigerinus artificial culture. The method includes: placing hatched tadpoles into a cultivation pond according to cultivation density of 3-4/liter, using feed containing 0.08-0.12 milligram of testosterone or letrozole in each gram to feed the tadpoles under 29-34 DEG C for two weeks, and then using feed which does not contain the testosterone or letrozole to feed the tadpoles until metamorphosis is completed. By the method, the sex ratio after the metamorphosis can be adjusted effectively, and the cultivation efficiency of hoplobatrachus tigerinus can be increased greatly. Compared with feeding under natural conditions, the method has the advantages that a metamorphosis cycle can be shortened greatly, and metamorphosis rate can be increased.
Description
Technical field
The present invention relates to a kind of control method and application of Mercuric chloride sex ratio, belong to Mercuric chloride artificial culture technology field.
Background technology
Mercuric chloride is not only country of China second class protection animal, and is a kind of important economic batrachia, all has certain distribution in south China farmland.Mercuric chloride build is very large, delicious meat, becomes the main amphibian that south China is captured, and the individual consumption of wild Mercuric chloride that only Guangdong, Guangxi two province consume reaches hundreds of ton.In addition, along with being constantly destroyed of Migration environment, Mercuric chloride population quantity is caused increasingly to reduce.
Therefore, propagating artificially of Mercuric chloride obtains very fast development in southern some areas.Many nests Mercuric chloride tadpole of collecting normally is positioned in outdoor pool and propagates artificially by major part raiser, in its pool, water temperature can have larger lability by the impact of temperature, these factors can reduce the distortion ratio of Mercuric chloride tadpole, extend the abnormal time.Mercuric chloride has strong intraspecific competition, and particularly the fight in breeding process of the male frog can cause individual damage, even dead, the male frogs a large amount of before First Year is passed the winter is dead because fighting, cause in the next one male frog quantity breeding period few like this, mating is just few, and breeding success rate will reduce.Although female frog individuality is comparatively large, have more fat-body and larger ovary tissue to occupy comparatively weight in its abdominal cavity, and these are organized and all can not eat, and fat-body in male frog abdominal cavity is relatively less, process of slaughtering is also relatively convenient.Therefore, although the female frog is a bit larger tham the male frog, the male frog is more suitable for cultivation, but will select suitable cultivation density and cultivation ratio, and density is crossed conference and caused interspecies competition, and density crosses the income that can affect raiser at least.At present, Mercuric chloride propagate neither one standard so far artificially, lack the improved seeds of artificially breeding, there is certain difficulty in the froglet that particularly the satisfactory sex ratio of output and state are better, quality is larger, this will have a strong impact on the follow-up breeding process of Mercuric chloride and market economy benefit thereof.
The sex ratio of Mercuric chloride, also can by the impact of water body exogenous hormone not only by the impact of temperature.Different temperatures raises Mercuric chloride tadpole can change the froglet sex ratio of raising population.Testosterone is a kind of androgenic hormone, and Letrozole is a kind of arimedex, and can effectively suppress the testosterone in body to transform to estradiol, these two kinds of hormones effectively can increase the quantity of male offspring.But on the one hand, the body weight impact of these medicines on the distortion ratio of tadpole, abnormal time and output froglet is unpredictable, and on the other hand, Mercuric chloride cultivation froglet sex ratio of institute's output under the yard feeding pattern of extensive style also often cannot reach expection requirement.
Summary of the invention
For the irrational problem of sex ratio after Mercuric chloride metamorphosis in solution prior art, the invention provides a kind of control method of Mercuric chloride sex ratio, the technical scheme taked is as follows:
The object of the present invention is to provide a kind of control method of Mercuric chloride sex ratio, the method is rendered to according to a certain percentage in culturing pool by the tadpole hatched, use the feed being added with testosterone or Letrozole, raise a period of time at 29-34 DEG C after, the feed used instead not containing testosterone or Letrozole continues to raise until metamorphosis completes.
Preferably, described the tadpole hatched to be rendered in culturing pool according to a certain percentage, refer to according to 3-4 tail/liter stocking density the tadpole hatched is rendered in culturing pool.
Preferably, described testosterone, every day, addition was 0.08-0.12 milligram/gram feed.
Preferably, described Letrozole, every day, addition was 0.08-0.12 milligram/gram feed.
Preferably, describedly at 29-34 DEG C, raise a period of time, feeding time is 2 weeks.
Preferably, the daily ration of feeding between feeding period is the 5-10% of whole pond tadpole body weight.
Described Mercuric chloride period of metamorphosis breeding method, step is as follows:
1) adjust the temperature of rearing pool at 29-34 DEG C, and control fluctuating temperature and be no more than 1 DEG C, according to 3-4 tail/liter cultivation density, in culturing pool, throw in the tadpole hatched;
2) according to the daily ration of feeding of whole pond tadpole body weight 5-10%, contain the feed of 0.08-0.12 milligram testosterone or 0.08-0.12 milligram Letrozole in throwing in every gram, raise 2 weeks;
3), after raising two weeks, the feed continuation raising changed not containing testosterone or Letrozole completes to tadpole metamorphosis.
Described either method is applied also within protection scope of the present invention in the raising of Mercuric chloride.
The beneficial effect that the present invention obtains is as follows:
The present invention is by raising temperature and the diet Mercuric chloride tadpole containing testosterone or Letrozole, not only the body weight of these medicines on the distortion ratio of tadpole, abnormal time and output froglet does not affect, but also the effect of Effective Regulation output froglet sex ratio can be played, can be raiser and control Mercuric chloride cultured populations sex ratio application method is provided.In addition with cultivate compared with Mercuric chloride tadpole under outdoor natural temperature, there is higher distortion ratio and shorter abnormal time (table 1).The distortion ratio of the Mercuric chloride tadpole cultivated under outdoor natural temperature condition can be low to moderate 13.3%, and the abnormal time is 52 days, and Mercuric chloride distortion ratio of the present invention is the highest is increased to 68%, abnormal time shorten to 21 day.
Accompanying drawing explanation
Fig. 1 is 29 DEG C of chart of percentage comparisons of raising lower Mercuric chloride male and female froglet.
Fig. 2 is 29 DEG C and raises and the chart of percentage comparison of Mercuric chloride male and female froglet under the process of testosterone feed.
Fig. 3 is 29 DEG C and raises and the chart of percentage comparison of Mercuric chloride male and female froglet under the process of Letrozole feed.
Fig. 4 is 34 DEG C of chart of percentage comparisons of raising lower Mercuric chloride male and female froglet.
Fig. 5 is 34 DEG C and raises and the chart of percentage comparison of Mercuric chloride male and female froglet under the process of testosterone feed.
Fig. 6 is 34 DEG C and raises and the chart of percentage comparison of Mercuric chloride male and female froglet under the process of Letrozole feed.
Embodiment
Below in conjunction with specific embodiment, the present invention will be further described, but the present invention is not by the restriction of embodiment.
Following examples material therefor, reagent, method and instrument, without specified otherwise, be this area conventional material, reagent, method and instrument, skilled person all obtains by commercial channel.
The present invention's feed used is No. 0 Bullfrog feedstuff (moisture≤12.0, crude protein >=42.0, crude fat >=3.0, raw fiber≤4.0, coarse ash≤18.0, calcium >=1.5, total phosphorus >=1.0, salt≤3.0) that Ningbo Tianbang Co., Ltd produces.
Embodiment 1
1) inventor carries out heat treatment to 3 pool inner waters with heater, and make water temperature be stabilized in 24 DEG C, 29 DEG C and 34 DEG C respectively, water temperature fluctuates up and down and is no more than 1 DEG C.Inventor will collect the Mercuric chloride tadpole of just hatching, by 3 tails/liter density put into 3 indoor culture ponds respectively, the tadpole in each culturing pool adds up to 540 tails.
2) get 50 tail tadpoles every day at random carry out weighing and estimate tadpole gross weight in pond, the daily ration, feeding quantity of every day calculates by the 5%-10% of tadpole gross weight.Tadpole from the 24 DEG C of water temperatures abnormal time is 44 – 62 days, and distortion ratio is 16%, and froglet body weight is 1.46-3.36 gram; Tadpole from the 29 DEG C of water temperatures abnormal time is 21-30 days, and distortion ratio is 77%, and froglet body weight is 0.90-1.97 gram; Tadpole from the 34 DEG C of water temperatures abnormal time is 19-25 days, and distortion ratio is 73%, and froglet body weight is 0.92-1.87 gram.
3) above-mentioned data display, water temperature on abnormal time of Mercuric chloride tadpole, distortion ratio and the impact of froglet body weight significantly, although the metamorphosis of Mercuric chloride tadpole becomes the body weight of froglet the heaviest under 24 DEG C of water temperatures, but distortion ratio is minimum and the abnormal time is longer, therefore should avoid in actual applications carrying out tadpole raising by 24 DEG C of water temperatures.Under 29 DEG C and 34 DEG C of water temperatures, abnormal time of Mercuric chloride tadpole, distortion ratio and froglet size are substantially close, but the property ratio of its output froglet there are differences, 29 DEG C of water temperature institute output froglet female and male ratios are 1:1 substantially, and the female and male ratio of 34 DEG C of water temperature institute output froglets is 1:3.
Embodiment 2
1) carry out heat treatment to 6 pool inner waters with heater, make 3 pool inner water temperature be stabilized in 29 DEG C, another 3 pool inner water temperature are stabilized in 34 DEG C, and water temperature fluctuates up and down and is no more than 1 DEG C.Inventor will collect the Mercuric chloride tadpole of just hatching, by 4 tails/liter density put into 6 indoor culture ponds respectively, the tadpole in each culturing pool adds up to 720 tails.
2) get 50 tail tadpoles every day at random carry out weighing and estimate tadpole gross weight in pond, the daily ration, feeding quantity of every day calculates by the 5%-10% of tadpole gross weight.
3) testosterone is got or Letrozole 0.08-0.12 milligram is dissolved in 100 milliliters of absolute alcohols, the absolute alcohol watering can of drug containing is evenly sprayed on 1 gram of feed surface, the feed of medicine sprinkling is had to be positioned in 65 DEG C of baking ovens fully dry, alcohol is volatilized completely, be configured to content be 0.08-0.12 milligram/gram testosterone or Letrozole feed, chow diet does not add any medicine.Throw in respectively in pond under each water temperature process chow diet, 0.08-0.12 milligram/gram testosterone feed and 0.08-0.12 milligram/gram Letrozole feed, throw in 2 weeks continuously, all throw in chow diet afterwards.Tadpole from the 29 DEG C of process abnormal time is 23-31 days, and distortion ratio is 57%, and froglet body weight is 1.06-2.03, and sex ration is about 1:1; Tadpole from the 34 DEG C of process abnormal time is 17-25 days, and distortion ratio is 56%, and froglet body weight is 1.11-1.77, and sex ration is about 1:3; The tadpole abnormal time from 29 DEG C and the process of testosterone feed is 23-35 days, and distortion ratio is 61%, and froglet body weight is 1.06-2.32 gram, and sex ration is about 3:7; The tadpole abnormal time from 34 DEG C and the process of testosterone feed is 17-25 days, and distortion ratio is 64%, and froglet body weight is 1.02-1.74 gram, is the male frog entirely; The tadpole abnormal time from 29 DEG C and the process of Letrozole feed is 23-35 days, and distortion ratio is 68%, and froglet body weight is 1.05-2.05 gram, and sex ration is about 1:5; The tadpole abnormal time from 34 DEG C and the process of Letrozole feed is 17-28 days, and distortion ratio is 60%, and froglet body weight is 1.09-1.76 gram, is the male frog entirely.
3) as can be seen from above-mentioned data, the distortion ratio of the Mercuric chloride tadpole that identical water temperature is raised, abnormal time and froglet body weight in testosterone feed and the process of Letrozole feed and chow diet process all without significant difference, but froglet property to compare difference under with or without pharmaceutical feed process very large.When water temperature is 29 DEG C, under chow diet process, male frog ratio is about 48%, and male frog ratio is 70% under the process of testosterone feed, and under the process of Letrozole feed, male frog ratio is 83%; When water temperature is 34 DEG C, under chow diet process, male frog ratio is about 74%, and male frog ratio is 100% under the process of testosterone feed, and under the process of Letrozole feed, male frog ratio is 100%.
Mercuric chloride tadpole is raised at 29 DEG C and 34 DEG C, and at first 2 weeks often the daily feed containing testosterone or Letrozole carry out feeding nursing, throw in chow diet afterwards, the froglet property of institute's output is than different.The abnormal time of Water Temperature Mercuric chloride tadpole, abnormal froglet body weight, but do not affect its distortion ratio; Feed process containing testosterone or Letrozole does not affect abnormal time of Mercuric chloride tadpole, distortion ratio, abnormal froglet body weight.Raise lower Mercuric chloride male and female froglet property ratio for 29 DEG C and be about 1:1 (Fig. 1); 29 DEG C is 3:7 (Fig. 2) with testosterone feed process output Mercuric chloride froglet female and male ratio; 29 DEG C are about 1:5 (Fig. 3) with Letrozole feed process output Mercuric chloride froglet female and male ratio; Raise lower Mercuric chloride male and female froglet property ratio for 34 DEG C and be about 1:3 (Fig. 4); 34 DEG C is male (Fig. 5) with testosterone feed process output Mercuric chloride froglet entirely, and 34 DEG C is male (Fig. 6) with Letrozole feed process output Mercuric chloride froglet entirely.Comparative result between the Mercuric chloride tadpole raised under the Mercuric chloride tadpole that the present invention raises and outdoor natural temperature is in table 1, and the Detailed Experimental feeding data of Mercuric chloride tadpole is in table 2.
Comparing between the Mercuric chloride tadpole that table 1 the present invention raises with the Mercuric chloride tadpole raised under outdoor natural temperature
Table 2 is Mercuric chloride Growth of Tadpoles situation under different water temperature and drug treating forage feed
Note: data mean value and Range Representation.
Although the present invention with preferred embodiment openly as above; but it is also not used to limit the present invention, any person skilled in the art, without departing from the spirit and scope of the present invention; can do various change and modification, what therefore protection scope of the present invention should define with claims is as the criterion.
Claims (8)
1. the control method of a Mercuric chloride sex ratio, it is characterized in that, the tadpole hatched is rendered in culturing pool according to a certain percentage, use the feed being added with testosterone or Letrozole, raise a period of time at 29-34 DEG C after, the feed used instead not containing testosterone or Letrozole continues to raise until metamorphosis completes.
2. the control method of a kind of Mercuric chloride sex ratio according to claim 1, it is characterized in that, described the tadpole hatched to be rendered in culturing pool according to a certain percentage, refer to according to 3-4 tail/liter stocking density the tadpole hatched is rendered in culturing pool.
3. the control method of a kind of Mercuric chloride sex ratio according to claim 1, is characterized in that, described testosterone, and every day, addition was 0.08-0.12 milligram/gram feed.
4. the control method of a kind of Mercuric chloride sex ratio according to claim 1, is characterized in that, described Letrozole, and every day, addition was 0.08-0.12 milligram/gram feed.
5. the control method of a kind of Mercuric chloride sex ratio according to claim 1, is characterized in that, describedly at 29-34 DEG C, raises a period of time, and feeding time is 2 weeks.
6. the control method of a kind of Mercuric chloride sex ratio according to claim 1, is characterized in that, the daily ration of feeding between feeding period is the 5-10% of whole pond tadpole body weight.
7. the control method of a kind of Mercuric chloride sex ratio according to claim 1, it is characterized in that, step is as follows:
1) adjust the temperature of rearing pool at 29-34 DEG C, and control fluctuating temperature and be no more than 1 DEG C, according to 3-4 tail/liter cultivation density, in culturing pool, throw in the tadpole hatched;
2) according to the daily ration of feeding of whole pond tadpole body weight 5-10%, contain the feed of 0.08-0.12 milligram testosterone or 0.08-0.12 milligram Letrozole in throwing in every gram, raise 2 weeks;
3), after raising two weeks, the feed continuation raising changed not containing testosterone or Letrozole completes to tadpole metamorphosis.
8. either method described in claim 1-7 is applied in the raising of Mercuric chloride.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105941331A (en) * | 2016-06-30 | 2016-09-21 | 湖南文理学院 | Efficient breeding method for triploid pure lines of snails |
| CN106106279A (en) * | 2016-06-30 | 2016-11-16 | 湖南文理学院 | A kind of raising bream, Carnis Megalobramae triploid incubation rate method |
| CN106106280A (en) * | 2016-06-30 | 2016-11-16 | 湖南文理学院 | A kind of improve crucian carp, the method for Cyprinus carpio male tetraploid incubation rate |
| CN106135093A (en) * | 2016-06-30 | 2016-11-23 | 湖南文理学院 | A kind of method improving Mylopharyngodon piceus triploid incubation rate |
| CN106135089A (en) * | 2016-06-30 | 2016-11-23 | 湖南文理学院 | The male tetraploid of Siniperca chuatsi is sheerly efficient breeding method |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105941331A (en) * | 2016-06-30 | 2016-09-21 | 湖南文理学院 | Efficient breeding method for triploid pure lines of snails |
| CN106106279A (en) * | 2016-06-30 | 2016-11-16 | 湖南文理学院 | A kind of raising bream, Carnis Megalobramae triploid incubation rate method |
| CN106106280A (en) * | 2016-06-30 | 2016-11-16 | 湖南文理学院 | A kind of improve crucian carp, the method for Cyprinus carpio male tetraploid incubation rate |
| CN106135093A (en) * | 2016-06-30 | 2016-11-23 | 湖南文理学院 | A kind of method improving Mylopharyngodon piceus triploid incubation rate |
| CN106135089A (en) * | 2016-06-30 | 2016-11-23 | 湖南文理学院 | The male tetraploid of Siniperca chuatsi is sheerly efficient breeding method |
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