CN104255615B - It is a kind of that soft thorn is naked splits buttocks fish artificial fecundation method - Google Patents
It is a kind of that soft thorn is naked splits buttocks fish artificial fecundation method Download PDFInfo
- Publication number
- CN104255615B CN104255615B CN201410537312.6A CN201410537312A CN104255615B CN 104255615 B CN104255615 B CN 104255615B CN 201410537312 A CN201410537312 A CN 201410537312A CN 104255615 B CN104255615 B CN 104255615B
- Authority
- CN
- China
- Prior art keywords
- fish
- naked
- water
- parent population
- splits
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241000251468 Actinopterygii Species 0.000 title claims abstract description 109
- 210000001217 buttock Anatomy 0.000 title claims abstract description 36
- 238000000034 method Methods 0.000 title claims abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 60
- 230000037396 body weight Effects 0.000 claims abstract description 17
- 238000009395 breeding Methods 0.000 claims abstract description 14
- 230000001488 breeding effect Effects 0.000 claims abstract description 14
- 238000011534 incubation Methods 0.000 claims abstract description 11
- 230000009027 insemination Effects 0.000 claims abstract description 10
- 230000032696 parturition Effects 0.000 claims abstract description 7
- 238000002360 preparation method Methods 0.000 claims abstract description 4
- 238000002347 injection Methods 0.000 claims description 29
- 239000007924 injection Substances 0.000 claims description 29
- 230000001568 sexual effect Effects 0.000 claims description 15
- 235000013601 eggs Nutrition 0.000 claims description 13
- 210000001015 abdomen Anatomy 0.000 claims description 12
- 230000003203 everyday effect Effects 0.000 claims description 12
- 230000012447 hatching Effects 0.000 claims description 11
- 241000252233 Cyprinus carpio Species 0.000 claims description 9
- 102000002322 Egg Proteins Human genes 0.000 claims description 9
- 108010000912 Egg Proteins Proteins 0.000 claims description 9
- 210000004681 ovum Anatomy 0.000 claims description 9
- 230000000384 rearing effect Effects 0.000 claims description 9
- 210000000582 semen Anatomy 0.000 claims description 9
- 208000027418 Wounds and injury Diseases 0.000 claims description 6
- 229940088597 hormone Drugs 0.000 claims description 6
- 239000005556 hormone Substances 0.000 claims description 6
- 238000007689 inspection Methods 0.000 claims description 6
- 210000000006 pectoral fin Anatomy 0.000 claims description 6
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 3
- 230000006378 damage Effects 0.000 claims description 3
- 230000012173 estrus Effects 0.000 claims description 3
- 210000003746 feather Anatomy 0.000 claims description 3
- 208000014674 injury Diseases 0.000 claims description 3
- 230000035800 maturation Effects 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 210000001672 ovary Anatomy 0.000 claims description 3
- 229910052760 oxygen Inorganic materials 0.000 claims description 3
- 239000001301 oxygen Substances 0.000 claims description 3
- 238000012216 screening Methods 0.000 claims description 3
- 230000000638 stimulation Effects 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 230000007306 turnover Effects 0.000 claims description 3
- 230000003442 weekly effect Effects 0.000 claims description 3
- 239000004744 fabric Substances 0.000 claims description 2
- 239000011152 fibreglass Substances 0.000 claims description 2
- 239000000463 material Substances 0.000 claims description 2
- 230000004720 fertilization Effects 0.000 abstract description 2
- 238000011109 contamination Methods 0.000 abstract 1
- 238000011144 upstream manufacturing Methods 0.000 description 4
- 241000252206 Cypriniformes Species 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 206010003694 Atrophy Diseases 0.000 description 1
- 102000011022 Chorionic Gonadotropin Human genes 0.000 description 1
- 108010062540 Chorionic Gonadotropin Proteins 0.000 description 1
- 241000251464 Coelacanthiformes Species 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 229920000832 Cutin Polymers 0.000 description 1
- 241000252210 Cyprinidae Species 0.000 description 1
- 101001109685 Homo sapiens Nuclear receptor subfamily 5 group A member 2 Proteins 0.000 description 1
- 206010020675 Hypermetropia Diseases 0.000 description 1
- 206010062767 Hypophysitis Diseases 0.000 description 1
- 102000029749 Microtubule Human genes 0.000 description 1
- 108091022875 Microtubule Proteins 0.000 description 1
- 102100022669 Nuclear receptor subfamily 5 group A member 2 Human genes 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- OFOBLEOULBTSOW-UHFFFAOYSA-N Propanedioic acid Natural products OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 description 1
- 241000065747 Schizopygopsis Species 0.000 description 1
- 241001414461 Schizopygopsis malacanthus Species 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 230000037444 atrophy Effects 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- CEAZRRDELHUEMR-UHFFFAOYSA-N gentamicin Chemical compound O1C(C(C)NC)CCC(N)C1OC1C(O)C(OC2C(C(NC)C(C)(O)CO2)O)C(N)CC1N CEAZRRDELHUEMR-UHFFFAOYSA-N 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 description 1
- 239000011976 maleic acid Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 210000004688 microtubule Anatomy 0.000 description 1
- 239000005445 natural material Substances 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 229940064298 pregnyl Drugs 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 229910021653 sulphate ion Inorganic materials 0.000 description 1
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Farming Of Fish And Shellfish (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
Abstract
The invention provides that a kind of soft thorn is naked splits buttocks fish artificial fecundation method, the steps include: 1, select free of contamination water quality; 2, with the net mode of catching collect wild soft thorn naked split buttocks fish in support parent population raise and train; 3, select that milter body weight is not less than 300g/ tail, raun body weight is not less than 400g/ tail physique is healthy and strong, anosis to be split buttocks fish without wound cultivate as breeding parent population without the soft thorn of deformity is naked from naked the splitting buttocks fish reserve parent fish of the wild soft thorn of raising and train through 1 year; 4, the breeding parent population cultivated through 1 year carries out preparation of hastening parturition; 5, select sexually matured parent population and carry out artificial induced spawning; 6, to the parent population artificial insemination after artificial induced spawning; 7, flowing water method is adopted to carry out artificial incubation.Use that the present invention soft thorn is naked splits that buttocks fish spawning rate reaches 80.00%, fertilization rate 63.48%, incubation rate 68.15%, can realize that soft thorn is naked splits buttocks fish scale artificial culture, the soft thorn of available protecting is naked splits buttocks fish resource.
Description
Technical field
The present invention relates to that a kind of soft thorn is naked splits buttocks fish artificial fecundation method.
Background technology
Soft thorn is naked to be split buttocks fish (Schizopygopsismalacanthus) and is under the jurisdiction of Cypriniformes, Cyprinidae, Schizothoracinae, Schizopygopsis, local by name: native fish, smallmouth fish etc., belong to cold water fishes, like the flow inlet place of Hui Shuichu and the plateau lake perched at Heavenly Stems and Earthly Branches stream, dwell life in the battalion end, winter survives the winter in cave, crack of stone, and its distribution height can reach height above sea level 3000-4000 rice.Take vegetal bait as food, often on rock, scrape food algae and submerged plant chip etc. with the cutin edge of lower jaw prosperity, also eat the blade of the aquatic microtubule fasolculus plant of part simultaneously.Soft thorn is naked to be split buttocks fish and is distributed in China's Jinsha jiang River middle and upper reaches and Yalongjiang River upstream water system, and be the medium-sized economic fish that in upstream branching flow and plateau lake, output is maximum, its delicious meat, being the kind having cultivation potentiality in cold water fish, is also Upper Yangtze River Endemic fish.
The research work splitting buttocks fish about soft thorn is naked, first carried out Classification And Nomenclature Revision (.2000. Fauna Sinica Osteichthyes Cypriniformes (last volume) such as happy pendant fine jade. Beijing: Science Press: 373-375.), Cao Wenxuan etc. have carried out Morbidity investigation and biological study (1. Cao Wenxuan, 5 offer civilian .1962. western Sichuan Ganzi Aba area biology of fishes and fishing problem. Acta Hydrobiologica Sinica, 2:79-111. 2. Wu Yun flies, Wu Cui precious .1992 Qinghai-Tibet Platean fish. Chengdu: Sichuan science tech publishing house: 1-599. is farsighted .2012. Jinsha jiang River upstream Status of Fisher Resources and protection recklessly 3.. Postgraduate School, Chinese Academy of Sciences's master thesis, 1-91), Shen Danzhou, Hu Rui etc. have carried out Age and growth research (1. Shen Danzhou, He Chunlin, the naked Age Determination splitting buttocks fish of the soft thorn of the refined .2007. of Song Zhao. Sichuan animal, 26 (1): 124-125. 2. Hu Rui, Wang Jianwei, Tan Deqing, Miao Zhiguo, but the naked research splitting buttocks fish Age and growth of the victory soft thorn in upstream, state .2012. Jinsha jiang River. Sichuan animal, 31 (5): 708-712, 719.).Soft thorn is naked, and to split buttocks fish be the fish producing demersal egg in miniflow water, and along with Jinsha jiang River middle and upper reaches Construction of Step Hydropower Station, the atrophy of flowing water habitat, produces the naked existence splitting buttocks fish of soft thorn and have a strong impact on, but have no the research report splitting the artificial propagation of buttocks fish about soft thorn is naked at present.
Summary of the invention
The object of the invention is for above-mentioned Problems existing, provide that a set of soft thorn is naked splits buttocks fish artificial fecundation method, protect that soft thorn is naked splits buttocks fish resource, and realize that soft thorn is naked splits buttocks fish scale artificial culture.The present invention is achieved in that
1, water quality requirement: cultivate water is pure and fresh, pollution-free, and dissolved oxygen is not less than 5mg/L, pH value 6.5-7.5, water temperature 0-20 DEG C;
2, parent population collection and raise and train: collect in the net mode of catching that the wild soft thorn of more than body weight 200g is naked splits buttocks fish parent population in support, rendered to by reserve parent fish to raise and train in pond and carry out domesticating and cultivating, raising and train pool area is 100-1000m
2, at the bottom of pond, ratio reduces to 2-3%, and pond height is 1.5-2.0m, and the depth of water is 1-1.5m, and discharge is 0.1-0.2m
3/ s, every square metre of breeding density is no more than 3kg, and raising and train feed is carp commercial feed, protein content 35-45%, and every day, feeding volume was the 1-2% of fish body weight, and every day respectively throws something and feeds once sooner or later;
3, the screening of parent population and cultivation is bred: select that milter body weight is not less than 300g/ tail, raun body weight is not less than 400g/ tail physique is healthy and strong, anosis splits buttocks fish as breeding parent population without wound without the soft thorn of deformity is naked from naked the splitting buttocks fish reserve parent fish of the wild soft thorn of raising and train through 1 year, parent fish rearing pool area is 20-50m
2, at the bottom of pond, ratio reduces to 1-2%, and pond height is 1.5-2.0m, and the depth of water is 1-1.5m, and discharge is 0.1-0.2m
3/ s, every square metre of breeding density is no more than 3kg, and raising and train feed is carp commercial feed, protein content 35-45%, and every day, feeding volume was the 1-4% of fish body weight, and every day respectively throws something and feeds once sooner or later;
4, to hasten parturition preparation: the breeding parent population cultivated through 1 year, in the 3-5 month, the sexual maturity situation of inspection 1 parent population is drawn in the net weekly in parent fish rearing pond, inspection method is: by the raun fish body of maturation upwards, slightly dew face, ovary profile is obviously seen in both sides, presses belly with have gentle hands, and soft high resilience is sexual maturity; Milter " pearl star " is obvious, and the gap between anal fin 4-6 root branch fin ray is strengthened, and be significantly hook-shaped in end specialization, hand touches body surface very strong harsh feeling, and upwards, light pressure belly both sides, have milky dense thick seminal fluid to flow out for sexual maturity to fish body belly; Before hastening parturition, strengthen parent fish rearing Chi Shui turnover amount, Chi Shui exchange per hour once, promotes parent population sexual maturity;
5, artificial induced spawning: select sexually matured raun, at its pectoral fin base portion injection fish ocyodinic, fish ocyodinic divides 1-4 injection, if 2-4 injection, with the 1st injected dose for benchmark, 2nd time injected dose is higher than the 1st time 1 times, 3rd time injected dose is higher than the 2nd time 1 times, the 4th injected dose is higher than the 3rd time 1 times, 24 hours double injection time intervals, the active situation of raun is observed after per injection, if there is phenomenon of laying eggs, just carry out artificial insemination, no longer carry out injection below, milter is also injected at its pectoral fin base portion, fish ocyodinic consumption is the half of raun, the no longer injection fish ocyodinic that milter sexual maturity is good, Central-South No. 3 of fish PG, LRH, DOM, HCG, fish hormone selected by fish ocyodinic, according to developmental state two kinds or three kinds of fish ocyodinic compatibilities of parent population, various fish ocyodinic injected dose is respectively PG:1-20mg/kg, LRH:1-20 μ g/kg, DOM:1-20mg/kg, HCG:500-1000IU/kg, fish hormone Central-South No. 3: 1-10ml/kg, as 1 injection, injection compatibility is LRH10 μ g/kg, HCG1000IU/kg, DOM5mg/kg, as point 3 injections, first time injection fish LRH1 μ g/kg, DOM1mg/kg, second time injection fish LRH2 μ g/kg, DOM2mg/kg, third time injection fish LRH4 μ g/kg, DOM4mg/kg, HCG1000IU/kg,
6, artificial insemination, puts into spawning pond by the parent population of having injected fish ocyodinic, spawning pond area 1-3m
2, pond height 0.7-1.0m, about depth of water 0.5m, the same pond of male and female, ceaselessly put stimulation by running water, observe parent population active situation at any time, when raun oestrus reach a climax time, if light pressure raun belly, fish-egg flows out, raun is mentioned, wipe body surface moisture, squeeze in ovum engagement, exist side by side and to mention by milter, wipe body surface moisture, rapidly seminal fluid is clamp-oned in basin, fish-egg 1-2 minute is stirred with feather, smart ovum is fully mixed, add a small amount of clear water again, stir slightly, leave standstill 1-2 minute, then ovum is washed 3-6 time with clear water, wash away the water of seminal fluid, add water and be placed on lucifuge place, sunlight and light direct projection should be avoided in whole operating process, prevent ultraviolet to the injury of sperm and fertilized egg, after artificial insemination, rehabilitation cultivation is carried out to parent population in postpartum,
7, artificial incubation: adopt Lotic hatching, incubator box is put into incubation tank, puts into fertilized egg after adding water, it is 3-6 Li/㎝ that each incubator box puts into density
2hatch, incubator box depth of water 10-15cm, hatching water quality requirement is with step 1, and at water temperature 12-18 DEG C, through the hatching of 149-183 hour, fertilized egg just starts rupture of membranes and emerges.
Using method of the present invention to carry out, soft thorn is naked splits the artificial propagation of buttocks fish, and soft thorn is naked splits that buttocks fish spawning rate reaches 80.00%, fertilization rate 63.48%, incubation rate 68.15%.Can realize that soft thorn is naked splits buttocks fish scale artificial culture, the soft thorn of available protecting is naked splits buttocks fish resource.
Embodiment
1, water quality requirement: cultivate water is pure and fresh, pollution-free, and dissolved oxygen is not less than 5mg/L, pH value 6.5-7.5, water temperature 0-20 DEG C, springwater, underground water, streams water, reservoir bottom water can be selected in water source;
2, parent population collection and raise and train: collect in the net mode of catching that the wild soft thorn of more than body weight 200g is naked splits buttocks fish parent population in support, rendered to by reserve parent fish to raise and train in pond and carry out domesticating and cultivating, raising and train pool area is 100-1000m
2, at the bottom of pond, ratio reduces to 2-3%, is convenient to release water, and pond height is 1.5-2.0m, and the depth of water is 1-1.5m, and discharge is 0.1-0.2m
3/ s, every square metre of breeding density is no more than 3kg, and raising and train pond can be cement pit, also can be the fishpond of natural material, build up and prevent escaping facility, be equipped with culvert system.Raising and train feed is carp commercial feed, and protein content 35-45% can select the carp commercial feed that Tong Wei feed companies produces; Every day, feeding volume was the 1-2% of fish body weight, and every day respectively throws something and feeds once sooner or later;
3, the screening of parent population and cultivation is bred: select that milter body weight is not less than 300g/ tail, raun body weight is not less than 400g/ tail physique is healthy and strong, anosis splits buttocks fish as breeding parent population without wound without the soft thorn of deformity is naked from naked the splitting buttocks fish reserve parent fish of the wild soft thorn of raising and train through 1 year, parent fish rearing pool area is 20-50m
2, at the bottom of pond, ratio reduces to 1-2%, is convenient to release water, and pond height is 1.5-2.0m, and the depth of water is 1-1.5m, and be equipped with and prevent escaping facility and culvert system, discharge is 0.1-0.2m
3/ s, every square metre of breeding density is no more than 3kg, and raising and train feed is carp commercial feed, and protein content 35-45% can select the carp commercial feed that Tong Wei feed companies produces; Every day, feeding volume was the 1-4% of fish body weight, and every day respectively throws something and feeds once sooner or later;
4, to hasten parturition preparation: the breeding parent population cultivated through 1 year, in the 3-5 month, the sexual maturity situation of inspection 1 parent population is drawn in the net weekly in parent fish rearing pond, inspection method is: by the raun fish body of maturation upwards, slightly dew face, ovary profile is obviously seen in both sides, presses belly with have gentle hands, and soft high resilience is sexual maturity; Milter " pearl star " is obvious, and the gap between anal fin 4-6 root branch fin ray is strengthened, and is significantly hook-shaped in end specialization, hand touches body surface very strong harsh feeling, and fish body belly upwards, gently presses belly both sides, have milky dense thick seminal fluid to flow out for sexual maturity, it is best for meeting the water person that do not scatter rapidly; Before hastening parturition, strengthen parent fish rearing Chi Shui turnover amount, Chi Shui exchange per hour once, promotes parent population sexual maturity;
5, artificial induced spawning: select sexually matured raun, at its pectoral fin base portion injection fish ocyodinic, fish ocyodinic divides 1-4 injection, if 2-4 injection, with the 1st injected dose for benchmark, 2nd time injected dose is higher than the 1st time 1 times, 3rd time injected dose is higher than the 2nd time 1 times, the 4th injected dose is higher than the 3rd time 1 times, 24 hours double injection time intervals, the active situation of raun is observed after per injection, if there is phenomenon of laying eggs, just carry out artificial insemination, no longer carry out injection below, milter is also injected at its pectoral fin base portion, fish ocyodinic consumption is the half of raun, the no longer injection fish ocyodinic that milter sexual maturity is good, fish ocyodinic can select fish PG (carp brain hypophysis-Peng Liuyang road, wuchang, wuhan district aquatic products fishing estovers business department provides), LRH (injection luteotropin analog releasing hormone-Ningbo Sansheng Pharmaceutical Co., Ltd. produces), DOM (maleic acid DOM-Ningbo Sansheng Pharmaceutical Co., Ltd. produces), HCG (pregnyl ampoule-Ningbo Sansheng Pharmaceutical Co., Ltd. produces), fish is with Central-South No. 3 of hormone (Guangzhou Central-South Bioisystech Co., Ltd produce), according to developmental state two kinds or three kinds of fish ocyodinic compatibilities of parent population, various fish ocyodinic injected dose is respectively PG:1-20mg/kg, LRH:1-20 μ g/kg, DOM:1-20mg/kg, HCG:500-1000IU/kg, fish hormone Central-South No. 3: 1-10ml/kg,
6, artificial insemination, puts into spawning pond by the parent population of having injected fish ocyodinic, spawning pond area 1-3m
2, pond height 0.7-1.0m, about depth of water 0.5m, the same pond of male and female, ceaselessly put stimulation by running water, observe parent population active situation at any time, when raun oestrus reach a climax time, if light pressure raun belly, fish-egg flows out, raun is mentioned, wipe body surface moisture, squeeze in ovum engagement, exist side by side and to mention by milter, wipe body surface moisture, rapidly seminal fluid is clamp-oned in basin, fish-egg 1-2 minute is stirred with feather, smart ovum is fully mixed, add a small amount of clear water again, stir slightly, leave standstill 1-2 minute, then ovum is washed 3-6 time with clear water, wash away the water of seminal fluid, add water and be placed on lucifuge place, sunlight and light direct projection should be avoided in whole operating process, prevent ultraviolet to the injury of sperm and fertilized egg, after artificial insemination, to parent population injection compound gentamicine sulphate in postpartum (4000IU/kg) or penicillin (10000IU/kg), carry out rehabilitation cultivation,
7, artificial incubation: adopt Lotic hatching, incubator box is put into incubation tank, puts into fertilized egg after adding water, it is 3-6 Li/㎝ that each incubator box puts into density
2hatch, incubator box depth of water 10-15cm, hatching water quality requirement is with step 1, and at water temperature 12-18 DEG C, through the hatching of 149-183 hour, fertilized egg just starts rupture of membranes and emerges.Incubation tank and incubator box are that glass-reinforced plastic material is made, and specification is: hatching flute length 2-3m, wide 0.4m, dark 0.17m; The long 0.4m of incubator box, wide 0.4m, dark 0.15m, at incubator box liner 40 object screen cloth, water freely can exchange at in-out-snap.
Claims (2)
1. soft thorn is naked splits a buttocks fish artificial fecundation method, it is characterized in that:
(1) water quality requirement: cultivate water is pure and fresh, pollution-free, and dissolved oxygen is not less than 5mg/L, pH value 6.5-7.5, water temperature 0-20 DEG C;
(2) parent population collection and raise and train: collect in the net mode of catching that the wild soft thorn of more than body weight 200g is naked splits buttocks fish parent population in support, rendered to by reserve parent fish to raise and train in pond and carry out domesticating and cultivating, raising and train pool area is 100-1000m
2, at the bottom of pond, ratio reduces to 2-3%, and pond height is 1.5-2.0m, and the depth of water is 1-1.5m, and discharge is 0.1-0.2m
3/ s, every square metre of breeding density is no more than 3kg, and raising and train feed is carp commercial feed, protein content 35-45%, and every day, feeding volume was the 1-2% of fish body weight, and every day respectively throws something and feeds once sooner or later;
(3) screening of parent population and cultivation is bred: select that milter body weight is not less than 300g/ tail, raun body weight is not less than 400g/ tail physique is healthy and strong, anosis splits buttocks fish as breeding parent population without wound without the soft thorn of deformity is naked from naked the splitting buttocks fish reserve parent fish of the wild soft thorn of raising and train through 1 year, parent fish rearing pool area is 20-50m
2, at the bottom of pond, ratio reduces to 1-2%, and pond height is 1.5-2.0m, and the depth of water is 1-1.5m, and discharge is 0.1-0.2m
3/ s, every square metre of breeding density is no more than 3kg, and raising and train feed is carp commercial feed, protein content 35-45%, and every day, feeding volume was the 1-4% of fish body weight, and every day respectively throws something and feeds once sooner or later;
(4) to hasten parturition preparation: the breeding parent population cultivated through 1 year, in the 3-5 month, the sexual maturity situation of inspection 1 parent population is drawn in the net weekly in parent fish rearing pond, inspection method is: by the raun fish body of maturation upwards, slightly dew face, ovary profile is obviously seen in both sides, presses belly with have gentle hands, and soft high resilience is sexual maturity; Milter " pearl star " is obvious, and the gap between anal fin 4-6 root branch fin ray is strengthened, and be significantly hook-shaped in end specialization, hand touches body surface very strong harsh feeling, and upwards, light pressure belly both sides, have milky dense thick seminal fluid to flow out for sexual maturity to fish body belly; Before hastening parturition, strengthen parent fish rearing Chi Shui turnover amount, Chi Shui exchange per hour once, promotes parent population sexual maturity;
(5) artificial induced spawning: select sexually matured raun, at its pectoral fin base portion injection fish ocyodinic, fish ocyodinic divides 1-4 injection, if 2-4 injection, with the 1st injected dose for benchmark, 2nd time injected dose is higher than the 1st time 1 times, 3rd time injected dose is higher than the 2nd time 1 times, the 4th injected dose is higher than the 3rd time 1 times, 24 hours double injection time intervals, the active situation of raun is observed after per injection, if there is phenomenon of laying eggs, just carry out artificial insemination, no longer carry out injection below, milter is also injected at its pectoral fin base portion, fish ocyodinic consumption is the half of raun, the no longer injection fish ocyodinic that milter sexual maturity is good, Central-South No. 3 of fish PG, LRH, DOM, HCG, fish hormone selected by fish ocyodinic, according to developmental state two kinds or three kinds of fish ocyodinic compatibilities of parent population, various fish ocyodinic injected dose is respectively PG:1-20mg/kg, LRH:1-20 μ g/kg, DOM:1-20mg/kg, HCG:500-1000IU/kg, fish hormone Central-South No. 3: 1-10ml/kg,
(6) artificial insemination, puts into spawning pond by the parent population of having injected fish ocyodinic, spawning pond area 1-3m
2, pond height 0.7-1.0m, about depth of water 0.5m, the same pond of male and female, ceaselessly put stimulation by running water, observe parent population active situation at any time, when raun oestrus reach a climax time, if light pressure raun belly, fish-egg flows out, raun is mentioned, wipe body surface moisture, squeeze in ovum engagement, exist side by side and to mention by milter, wipe body surface moisture, rapidly seminal fluid is clamp-oned in basin, fish-egg 1-2 minute is stirred with feather, smart ovum is fully mixed, add a small amount of clear water again, stir slightly, leave standstill 1-2 minute, then ovum is washed 3-6 time with clear water, wash away the water of seminal fluid, add water and be placed on lucifuge place, sunlight and light direct projection should be avoided in whole operating process, prevent ultraviolet to the injury of sperm and fertilized egg, after artificial insemination, rehabilitation cultivation is carried out to parent population in postpartum,
(7) artificial incubation: adopt Lotic hatching, incubator box is put into incubation tank, puts into fertilized egg after adding water, it is 3-6 Li/㎝ that each incubator box puts into density
2hatch, incubator box depth of water 10-15cm, the hatching same step of water quality requirement (1), at water temperature 12-18 DEG C, through the hatching of 149-183 hour, fertilized egg just starts rupture of membranes and emerges.
2. soft thorn according to claim 1 is naked splits buttocks fish artificial fecundation method, and it is characterized in that incubation tank and incubator box are that glass-reinforced plastic material is made, specification is: hatching flute length 2-3m, wide 0.4m, dark 0.17m; The long 0.4m of incubator box, wide 0.4m, dark 0.15m, at incubator box liner 40 object screen cloth.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410537312.6A CN104255615B (en) | 2014-10-13 | 2014-10-13 | It is a kind of that soft thorn is naked splits buttocks fish artificial fecundation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410537312.6A CN104255615B (en) | 2014-10-13 | 2014-10-13 | It is a kind of that soft thorn is naked splits buttocks fish artificial fecundation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104255615A CN104255615A (en) | 2015-01-07 |
| CN104255615B true CN104255615B (en) | 2016-03-02 |
Family
ID=52147302
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410537312.6A Active CN104255615B (en) | 2014-10-13 | 2014-10-13 | It is a kind of that soft thorn is naked splits buttocks fish artificial fecundation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104255615B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104585095A (en) * | 2015-01-20 | 2015-05-06 | 青岛龙宫海洋牧场生态修复工程有限公司 | Culturing method of aurelia scyphistoma |
| CN106538429A (en) * | 2016-11-15 | 2017-03-29 | 丽江林昊生态养殖有限公司 | A kind of naked cultural method for splitting buttocks fish of soft thorn |
| CN106818550A (en) * | 2016-12-28 | 2017-06-13 | 贵州锦润水产品有限责任公司 | A kind of naked cultural method for splitting buttocks fish of soft thorn |
| CN106879510B (en) * | 2017-04-12 | 2019-04-19 | 西藏自治区农牧科学院水产科学研究所 | The naked induced spawning method for splitting buttocks fish in Lhasa |
| CN116287289B (en) * | 2023-01-09 | 2023-10-13 | 中国长江三峡集团有限公司中华鲟研究所 | Identification method of white turtle germplasm and application of white turtle germplasm identification method in polyculture method of white turtle, soft-thorn nojirimi fish and red eye trout |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103875572B (en) * | 2014-04-10 | 2015-08-05 | 中国科学院昆明动物研究所 | It is a kind of that soft thorn is naked splits the artificial propagation of buttocks fish and hatch fish roe method |
-
2014
- 2014-10-13 CN CN201410537312.6A patent/CN104255615B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN104255615A (en) | 2015-01-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104255614B (en) | A kind of schizothoracin hard Juniperus formosana Pan naked carp artificial fecundation method | |
| CN101919359B (en) | Method for comprehensively regulating and controlling gonad mature ovulation of cheilinus undulates | |
| CN102077787B (en) | Artificial propagation technology for schizothorax grahami | |
| CN105075940B (en) | A kind of analysis of Cromileptes altivelis and the artificial hybridization breeding method of epinephelus lanceolatus fish | |
| CN104255615B (en) | It is a kind of that soft thorn is naked splits buttocks fish artificial fecundation method | |
| CN103931525B (en) | The artificial propagation of the naked skin-carp meat in Xinjiang and fry rearing method | |
| CN104221958B (en) | A kind of C. guichenoti induced spawning method | |
| CN107581106B (en) | A kind of artificial fecundation method of platax orbicularis | |
| CN103444610B (en) | Artificial propagation method for acrossocheilus yunnanensis | |
| CN105360028B (en) | The artificial fecundation method of east Triplophysa | |
| CN103960184B (en) | A kind of loach industrial artificial breeding improves the seedling-cultivating method of survival rate | |
| CN104642212A (en) | Artificial breeding method for onychostoma simus | |
| CN103461253B (en) | Yellow River fish artificial fecundation method | |
| CN101960996A (en) | Artificial breeding method of Scaphesthes macrolepis fry | |
| CN105284679A (en) | Ancherythroculter nigrocauda artificial breeding method | |
| CN106577393A (en) | Artificial propagation method for breeding acrossocheilus fasciatus | |
| CN106359195B (en) | A kind of offspring breeding method of vacricorhinus gerlachi | |
| CN101878741B (en) | Interbreeding method of hemibarbus labeo and hemibarbus maculatus | |
| CN106212333A (en) | A kind of artificial fecundation method of north palpus loach | |
| CN108967278A (en) | A kind of artificial fecundation method of Scatophagus argus (Linnaeus) | |
| CN106942096A (en) | A kind of many squamas shovel the artificial propagation breeding method of jaw fish | |
| CN104396813A (en) | Gymnocypris przewalskii full artificial breeding method | |
| CN104285855A (en) | Large-scale xenocypris davidi bleeker artificial reproduction method | |
| CN105265362A (en) | Cross breeding method for improving growth traits of slender mandarinfish | |
| CN112167118A (en) | Artificial propagation method of zier whitefish |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| ASS | Succession or assignment of patent right |
Owner name: YULONG CHONGJIANG HEZHONG CULTIVATION DEVELOPMENT Effective date: 20150114 |
|
| C10 | Entry into substantive examination | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20150114 Address after: Shigu Town, Lijiang city of Yunnan Province, 674199 ERON County Luwacun Gao Mingzu Applicant after: LIJIANG ZHENJIANG FISH BREEDING Co.,Ltd. Applicant after: YULONG COUNTY CHONGJIANGHE SPECIES BREEDING DEVELOPMENT CO.,LTD. Address before: Shigu Town, Lijiang city of Yunnan Province, 674199 ERON County Luwacun Gao Mingzu Applicant before: LIJIANG ZHENJIANG FISH BREEDING Co.,Ltd. |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20200929 Address after: 430000 Dongchuang Creative Park 3-104-1, No. 2 Xudong Second Road, Wuchang District, Wuhan City, Hubei Province Patentee after: WUHAN SINOECO TECHNOLOGY Co.,Ltd. Address before: Shigu Town, Lijiang city of Yunnan Province, 674199 ERON County Luwacun Gao Mingzu Patentee before: LIJIANG ZHENJIANG FISH BREEDING Co.,Ltd. Patentee before: YULONG COUNTY CHONGJIANGHE SPECIES BREEDING DEVELOPMENT Co.,Ltd. |
|
| TR01 | Transfer of patent right |