CN106879510B - The naked induced spawning method for splitting buttocks fish in Lhasa - Google Patents
The naked induced spawning method for splitting buttocks fish in Lhasa Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/003—Aquaria; Terraria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/60—Fish, e.g. seahorses; Fish eggs
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- A61K38/00—Medicinal preparations containing peptides
- A61K38/04—Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
- A61K38/08—Peptides having 5 to 11 amino acids
- A61K38/09—Luteinising hormone-releasing hormone [LHRH], i.e. Gonadotropin-releasing hormone [GnRH]; Related peptides
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
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Abstract
The present invention provides a kind of naked induced spawning methods for splitting buttocks fish in Lhasa, comprising the following steps: A, parent population selection；B, artificial induced spawning；C, artificial insemination；D, artificial incubation.This method is simple, strong operability, and practical application is good, and the naked scale for splitting buttocks fish in wild Lhasa and standardization artificial induced spawning may be implemented, promote the naked aquaculture development for splitting buttocks fish in Lhasa, effective protection and split buttocks wild resource using Lhasa is naked.
Description
Technical field
The invention belongs to technical field of aquaculture, and in particular to a kind of naked induced spawning method for splitting buttocks fish in Lhasa.
Background technique
Tibet possesses water resource abundant, is the water tower in the world.According to statistics, Tibet differs in size lake more than 2000,
More than 24000 km of the lake gross area2, account for about the 30% of the national lake gross area.On the vast plateau in Tibet, drainage area is greater than
10000km2River have more than 20 items, be greater than 2000km2River it is a plurality of 100, including Jinsha jiang River, the Lancang River, Nujiang, Ya Lu
The rivers such as Zang Bujiang, friend's song, Senge Zangbu rise or flow through Tibet.The fish being distributed in numerous rivers and lake have three
Big monoid is schizothoracin class, Triplophysa and Select-Committee section fish respectively.Wherein Lhasa is naked splits buttocks (Schizopygopsis
Younghusbandi) it is under the jurisdiction of Cypriniformes (Cypriniformes), Cyprinidae (Cyprinidae), Schizothoracinae
(Schizothoracinae), Schizopygopsis (Schizopygopsis) is commonly called as native fish, is the important original inhabitants' warp of Tibet plateau
One of Ji fish, belong to cold water fishes, dry Yarlung Zangbo River middle and upper reaches, tributary and its attached water body are mainly distributed on, for me
State endemic species.Lhasa is naked in nature splits buttocks raun more than milter, belongs to synchronous Type of Spawning in batches, lays eggs 1 time within 1 year, mainly
March~April is concentrated on, breeding period is shorter.In recent years, as Tibet's economy society is fast-developing, transportation network is more convenient,
Consumer market demand constantly increases, and it is extremely serious artificially to excessively capture phenomenon；In addition, being established on Yarlung Zangbo River mainstream and tributary
Power station hinders fish migration channel, and the naked stock number for splitting buttocks fish in Lhasa is forced to tend to decline.
Patent of invention CN201410142435.X discloses that a kind of soft thorn is naked to split the artificial propagation of buttocks fish and hatch fish roe method,
Patent of invention CN201310449845.4 discloses a kind of Yellow River fish artificial fecundation method, urging in two kinds of induced spawning methods
It produces composition of medicine and dosage is not suitable for the naked artificial induced spawning for splitting buttocks fish in Lhasa.
Summary of the invention
In order to realize the full artificial breeding technology of Lhasa schizothoracin, the present invention has carried out that Lhasa is naked to split buttocks artificial propagation techniques
Research, provides a kind of suitable for the naked induced spawning method for splitting buttocks fish in Lhasa, and this method is simple, and strong operability, practice is answered
It is good with property, the naked scale for splitting buttocks fish in wild Lhasa and standardization artificial induced spawning may be implemented.
Technical scheme is as follows:
A kind of naked induced spawning method for splitting buttocks fish in Lhasa, comprising the following steps:
A, parent population selects
Select it is healthy and strong, physically well develop, without obvious wound and without deformity wild Lhasa it is naked split buttocks parent population, choose parent
Fish carries out disinfection entering Chi Qianyong potassium permanganate, is then divided in parent population storage pond, holding pond by male and female and temporarily supports 1 day；Storage pond, holding pond is used in advance
It potassium permanganate soaking one week, is cleaned up later with clear water；The parent population storage pond, holding pond water temperature be 11~12 DEG C, dissolved oxygen be 6~
8mg/L, pH are 7.2~7.5.
By many years Yarlung Zangbo River fishery resources survey, it is found that Luo Liekao spawning ground water temperature in Lhasa is 11 DEG C or so, it is molten
Oxygen is higher than 6mg/L, and pH is to simulate its aquatic environment laid eggs under field conditions (factors) as far as possible between 7.1~7.6.
Preferably, the proportion of male and female parent population is 1:1.2, splits buttocks fish raun more than milter, institute since Lhasa is naked in nature
It is as much as possible to make good use of milter with when artificial induced spawning is bred, and can guarantee that female fish egg is preferably fertilized, guarantee it
Scale hasten parturition artificial induced spawning breeding.
B, artificial induced spawning
Using pectoral fin base portion injection method, injection dosage are as follows: 5 μ g luteotropin releasing hormone d-ala analogs (LRH-A2)/kg+
2.5mg carp pituitary (PG)/kg+1ml fish physiological saline；
The concentration of the fish physiological saline is 0.65 ﹪.
Raun is injected 2 times, for the first time injection 30%, interval second of injection 70% after 24 hours；Milter is injected 1 time, with
Second of injection of raun is synchronous to be carried out, and injection dosage halves.Female milter is put into togerther after second of oxytocic drug injection
In the spawning pond of glass reinforced plastic, parent population is observed every 4 hours, checks that circulation runs well, while opening water pump,
Carry out 20 minutes stimulation by running water；
Preferably, when injecting oxytocic drug, obstacle is wrapped up above syringe needle, exposed needle length is 0.5cm, in order to avoid advising
Syringe needle insertion is too deep when modelling artificial induced spawning, injures the vitals such as parent population heart.
Preferably, the flow velocity of the stimulation by running water is 0.1-0.2m/s, identical as nature spawning ground water velocity.
C, artificial insemination
The injection of second oxytocic drug for 24 hours after, check artificial induced spawning parent population, inseminated using dry method；
Raun body surface moisture is dried with dry towel first, movement is soft, prevents scratch, ovum, which is squeezed into diameter, is
In the white ceramic bowl of 30cm, milter sperm is extruded on ovum by same movement and method, is gently agitated for 10 seconds with feather, with wooden dipper along porcelain
Tub wall slowly adds a little clear water, has just flooded ovum preferably, is gently agitated for 20 seconds with feather again, later plus clear water is washed three times；
D, artificial incubation
Fertilized eggs are put into hatching frame, micro- Lotic hatching is carried out, control water temperature is 11~12 DEG C, and dissolved oxygen is 6~8mg/
L, pH are 7.2~7.5.
Preferably, it is the wooden hatching frame of 10 purposes, the hatching suitable for fish-egg that the hatching frame, which is mesh,.
Preferably, the ovum density of putting hatched in frame is 4.17 ten thousand Li/㎡.
Preferably, micro- Lotic hatching refers to that water flow is 1.5 cubes ms/h, and hatching effect is preferable.
The parent population storage pond, holding pond of step A of the present invention is 1.2m glass reinforced plastic round tank, and the spawning pond of step B is that diameter is
The glass reinforced plastic circle spawning pond of 2.5m.Glass reinforced plastic round tank surface is smooth first, and wild Lhasa schizothoracin has certain after being put into
Stress effect, prevent because stress due to abrade fish body body surface, diameter be 1.2m by Lhasa it is naked split buttocks fish separate temporarily support, be conducive to grasp
Make, facilitates management；The glass reinforced plastic circle spawning pond that 2.5m is selected after second needle is because male and female parent population is placed on one at this time
It rises, in addition, spawning pond has boost pump, certain water flow stimulation can be carried out to parent population.So in order to reach more preferable effect and side
Just operation just selects the spawning pond of diameter 1.2m storage pond, holding pond and diameter 2.5m.
The beneficial effects of the present invention are:
1, this method is simple, strong operability, and practical application is good, and the naked scale for splitting buttocks fish in wild Lhasa may be implemented
With standardization artificial induced spawning, promotes the naked aquaculture development for splitting buttocks fish in Lhasa, effective protection and split the wild money of buttocks using Lhasa is naked
Source.The naked artificial the mean spawning rate for splitting buttocks fish in Lhasa reaches 90.4 ﹪, average fertilization rate reached to 87 ﹪.
2, male and female parent population is separated and is temporarily supported 1 day in parent fish pond by the present invention, to adapt to artificial induced spawning environment, second of injection
After, then female milter put together, it may advantageously facilitate parent population sperm and ovum reach mature.
3, the present invention has been selected according to the naked characteristic for splitting buttocks fish in Lhasa suitable for the naked specific oxytocic drug for splitting buttocks fish in Lhasa
Combination and dosage, 5 μ g luteotropin releasing hormone d-ala analogs/kg+2.5mg carp pituitary/kg+1ml fish physiological saline obtain
To best spawning effect.
4,1:1.2 is set by the proportion of male and female parent population, is to split buttocks fish raun more than milter since Lhasa is naked in nature,
So when artificial induced spawning breeding, it is as much as possible to make good use of milter, and can guarantee that female fish egg is preferably fertilized, guarantee
Its scale hasten parturition artificial induced spawning breeding.
5, parent population storage pond, holding pond of the invention is 1.2m glass reinforced plastic round tank, and spawning pond is that the glass reinforced plastic that diameter is 2.5m is round
Spawning pond.Glass reinforced plastic round tank surface is smooth first, wild Lhasa schizothoracin be put into after have certain stress effect, prevent because
Stress and abrade fish body body surface, diameter be 1.2m by Lhasa it is naked split buttocks fish separate temporarily support, be conducive to operate, facilitate management；Second
The glass reinforced plastic circle spawning pond that 2.5m is selected after needle is because male and female parent population is put together at this time, in addition, spawning pond has
Boost pump can carry out certain water flow stimulation to parent population.So in order to reach more preferable effect and operation be facilitated just to select diameter
The spawning pond of 1.2m storage pond, holding pond and diameter 2.5m.
Specific embodiment
Property content is described in further detail for the essence of the present invention With reference to embodiment.
Embodiment 1
A kind of naked induced spawning method for splitting buttocks fish in Lhasa, comprising the following steps:
A, parent population selects
Select it is healthy and strong, physically well develop, without obvious wound and without deformity wild Lhasa it is naked split buttocks parent population, choose parent
Fish carries out disinfection entering Chi Qianyong potassium permanganate, is then divided in parent population storage pond, holding pond by male and female and temporarily supports 1 day；Storage pond, holding pond is used in advance
It potassium permanganate soaking one week, is cleaned up later with clear water；The parent population storage pond, holding pond water temperature is 11 DEG C, and dissolved oxygen 6mg/L, pH are
7.2；
B, artificial induced spawning
Using pectoral fin base portion injection method, injection dosage are as follows: 5 μ g luteotropin releasing hormone d-ala analogs/kg+2.5mg carp
Pituitary/kg+1ml fish physiological saline；
Raun is injected 2 times, for the first time injection 30%, interval second of injection 70% after 24 hours；Milter is injected 1 time, with
Second of injection of raun is synchronous to be carried out, and injection dosage halves.Female milter is put into togerther after second of oxytocic drug injection
In the spawning pond of glass reinforced plastic, parent population is observed every 4 hours, checks that circulation runs well, while opening water pump,
Carry out 20 minutes stimulation by running water；
C, artificial insemination
The injection of second oxytocic drug for 24 hours after, check artificial induced spawning parent population, inseminated using dry method；
D, artificial incubation
Fertilized eggs are put into hatching frame, micro- Lotic hatching is carried out, control water temperature is 11 DEG C, and dissolved oxygen 6mg/L, pH are
7.2。
Embodiment 2
A kind of naked induced spawning method for splitting buttocks fish in Lhasa, comprising the following steps:
A, parent population selects
Select it is healthy and strong, physically well develop, without obvious wound and without deformity wild Lhasa it is naked split buttocks parent population, choose parent
Fish carries out disinfection entering Chi Qianyong potassium permanganate, is then divided in parent population storage pond, holding pond by male and female and temporarily supports 1 day；Storage pond, holding pond is used in advance
It potassium permanganate soaking one week, is cleaned up later with clear water；The parent population storage pond, holding pond water temperature is 12 DEG C, and dissolved oxygen 8mg/L, pH are
7.5；
B, artificial induced spawning
Using pectoral fin base portion injection method, injection dosage are as follows: 5 μ g luteotropin releasing hormone d-ala analogs/kg+2.5mg carp
Pituitary/kg+1ml fish physiological saline；
Raun is injected 2 times, for the first time injection 30%, interval second of injection 70% after 24 hours；Milter is injected 1 time, with
Second of injection of raun is synchronous to be carried out, and injection dosage halves.Female milter is put into togerther after second of oxytocic drug injection
In the spawning pond of glass reinforced plastic, parent population is observed every 4 hours, checks that circulation runs well, while opening water pump,
Carry out 20 minutes stimulation by running water；
C, artificial insemination
The injection of second oxytocic drug for 24 hours after, check artificial induced spawning parent population, inseminated using dry method；
D, artificial incubation
Fertilized eggs are put into hatching frame, micro- Lotic hatching is carried out, control water temperature is 12 DEG C, and dissolved oxygen 8mg/L, pH are
7.5。
Embodiment 3
A kind of naked induced spawning method for splitting buttocks fish in Lhasa, comprising the following steps:
A, parent population selects
Select it is healthy and strong, physically well develop, without obvious wound and without deformity wild Lhasa it is naked split buttocks parent population, choose parent
Fish carries out disinfection entering Chi Qianyong potassium permanganate, is then divided in parent population storage pond, holding pond by male and female and temporarily supports 1 day；Storage pond, holding pond is used in advance
It potassium permanganate soaking one week, is cleaned up later with clear water；The parent population storage pond, holding pond water temperature is 11.5 DEG C, dissolved oxygen 7mg/L, pH
It is 7.3；
B, artificial induced spawning
Using pectoral fin base portion injection method, injection dosage are as follows: 5 μ g luteotropin releasing hormone d-ala analogs/kg+2.5mg carp
Pituitary/kg+1ml fish physiological saline；
Raun is injected 2 times, for the first time injection 30%, interval second of injection 70% after 24 hours；Milter is injected 1 time, with
Second of injection of raun is synchronous to be carried out, and injection dosage halves.Female milter is put into togerther after second of oxytocic drug injection
In the spawning pond of glass reinforced plastic, parent population is observed every 4 hours, checks that circulation runs well, while opening water pump,
Carry out 20 minutes stimulation by running water；
C, artificial insemination
The injection of second oxytocic drug for 24 hours after, check artificial induced spawning parent population, inseminated using dry method；
D, artificial incubation
Fertilized eggs are put into hatching frame, micro- Lotic hatching is carried out, control water temperature is 11.5 DEG C, and dissolved oxygen 7mg/L, pH are
7.3。
Embodiment 4
The present embodiment is on the basis of embodiment 1:
The proportion of male and female parent population is 1:1.2.
Embodiment 5
The present embodiment is on the basis of embodiment 1:
The proportion of male and female parent population is 1:1.2.
The parent population storage pond, holding pond of the step A is 1.2m glass reinforced plastic round tank, and the spawning pond of step B is the glass that diameter is 2.5m
Glass steel circle spawning pond.
Embodiment 6
The present embodiment is on the basis of embodiment 1:
The proportion of male and female parent population is 1:1.2.
The parent population storage pond, holding pond of the step A is 1.2m glass reinforced plastic round tank, and the spawning pond of step B is the glass that diameter is 2.5m
Glass steel circle spawning pond.
The step B wraps up obstacle when injecting oxytocic drug above syringe needle, exposed needle length is 0.5cm.
Embodiment 7
The present embodiment is on the basis of embodiment 1:
The proportion of male and female parent population is 1:1.2.
The parent population storage pond, holding pond of the step A is 1.2m glass reinforced plastic round tank, and the spawning pond of step B is the glass that diameter is 2.5m
Glass steel circle spawning pond.
The step B wraps up obstacle when injecting oxytocic drug above syringe needle, exposed needle length is 0.5cm.
The step B, the flow velocity of stimulation by running water are 0.1m/s.
Embodiment 8
The present embodiment is on the basis of embodiment 1:
The proportion of male and female parent population is 1:1.2.
The parent population storage pond, holding pond of the step A is 1.2m glass reinforced plastic round tank, and the spawning pond of step B is the glass that diameter is 2.5m
Glass steel circle spawning pond.
The step B wraps up obstacle when injecting oxytocic drug above syringe needle, exposed needle length is 0.5cm.
The step B, the flow velocity of stimulation by running water are 0.2m/s.
The dry method insemination refers to, dries raun body surface moisture with dry towel first, and movement is soft, prevents scratch skin
Skin is squeezed into ovum in the white ceramic bowl that diameter is 30cm, and milter sperm is extruded on ovum by same movement and method, gently with feather
Agitation 10 seconds, slowly adds a little clear water along ceramic bowl wall with wooden dipper, has just flooded ovum preferably, is gently agitated for 20 seconds with feather again, later
Clear water is added to wash three times.
Embodiment 9
The present embodiment is on the basis of embodiment 1:
The proportion of male and female parent population is 1:1.2.
The parent population storage pond, holding pond of the step A is 1.2m glass reinforced plastic round tank, and the spawning pond of step B is the glass that diameter is 2.5m
Glass steel circle spawning pond.
The step B wraps up obstacle when injecting oxytocic drug above syringe needle, exposed needle length is 0.5cm.
The step B, the flow velocity of stimulation by running water are 0.15m/s.
The dry method insemination refers to, dries raun body surface moisture with dry towel first, and movement is soft, prevents scratch skin
Skin is squeezed into ovum in the white ceramic bowl that diameter is 30cm, and milter sperm is extruded on ovum by same movement and method, gently with feather
Agitation 10 seconds, slowly adds a little clear water along ceramic bowl wall with wooden dipper, has just flooded ovum preferably, is gently agitated for 20 seconds with feather again, later
Clear water is added to wash three times.
The D step, the ovum density of putting hatched in frame is 4.1 ten thousand Li/㎡.
Embodiment 10
The present embodiment is on the basis of embodiment 1:
The proportion of male and female parent population is 1:1.2.
The parent population storage pond, holding pond of the step A is 1.2m glass reinforced plastic round tank, and the spawning pond of step B is the glass that diameter is 2.5m
Glass steel circle spawning pond.
The step B wraps up obstacle when injecting oxytocic drug above syringe needle, exposed needle length is 0.5cm.
The step B, the flow velocity of stimulation by running water are 0.16m/s.
The dry method insemination refers to, dries raun body surface moisture with dry towel first, and movement is soft, prevents scratch skin
Skin is squeezed into ovum in the white ceramic bowl that diameter is 30cm, and milter sperm is extruded on ovum by same movement and method, gently with feather
Agitation 10 seconds, slowly adds a little clear water along ceramic bowl wall with wooden dipper, has just flooded ovum preferably, is gently agitated for 20 seconds with feather again, later
Clear water is added to wash three times.
The D step, the ovum density of putting hatched in frame is 4.2 ten thousand Li/㎡.
Micro- Lotic hatching of the D step refers to that water flow is 1.5 cubes ms/h.
Embodiment 11
The present embodiment is on the basis of embodiment 1:
The proportion of male and female parent population is 1:1.2.
The parent population storage pond, holding pond of the step A is 1.2m glass reinforced plastic round tank, and the spawning pond of step B is the glass that diameter is 2.5m
Glass steel circle spawning pond.
The step B wraps up obstacle when injecting oxytocic drug above syringe needle, exposed needle length is 0.5cm.
The step B, the flow velocity of stimulation by running water are 0.12m/s.
The dry method insemination refers to, dries raun body surface moisture with dry towel first, and movement is soft, prevents scratch skin
Skin is squeezed into ovum in the white ceramic bowl that diameter is 30cm, and milter sperm is extruded on ovum by same movement and method, gently with feather
Agitation 10 seconds, slowly adds a little clear water along ceramic bowl wall with wooden dipper, has just flooded ovum preferably, is gently agitated for 20 seconds with feather again, later
Clear water is added to wash three times.
The D step, the ovum density of putting hatched in frame is 4.17 ten thousand Li/㎡.
Micro- Lotic hatching of the D step refers to that water flow is 1.5 cubes ms/h.
The hatching frame of the D step is that mesh is the wooden hatching frame of 10 purposes.
The selection of parent population of the present invention
Select it is healthy and strong, physically well develop, without obvious wound and without deformity parent population, select 0.25~0.65kg of specification,
Average out to 0.42kg；230 tail of milter, 0.25~0.56kg of specification, average out to 0.31kg, male and female proportion are 1:1.2；It will choose
Wild Lhasa it is naked split buttocks parent population and enter Chi Qianyong potassium permanganate carry out disinfection, the use of concentration is 20g/m3 soaking 10 minutes, then
It separates and is temporarily supported in diameter as in 1.2m glass reinforced plastic round tank, storage pond, holding pond uses potassium permanganate (20g/m3) soaking one in advance by male and female
It is cleaned up later with clear water in week, parent population storage pond, holding pond water temperature is 11~12 DEG C, and dissolved oxygen is 6~8mg/L, and pH is 7.2~7.5.
Parent population Sex judging of the present invention
Raun: raun individual is relatively large, and hand touches that body is smooth, and abdomen obviously expands, is soft, is flexible, gonopore is bright
Aobvious protrusion is mellow and full, anal fin least significant end fin ray not bifurcated.
Milter: milter individual is relatively small, and abdomen is flat, and it is coarse that hand touches body, is furnished with star-pursuing on pectoral fin to tail fin trunk,
Anal fin fin ray is flourishing, has apparent zigzag, and gently squeezing abdomen has the outflow of milky sperm, and can dissipate quickly in water
It opens.
On April 2nd, 2016, April 8, hasten parturition at April 12 in three batches.Preferable raun 51 is developed in selection for the first time
Tail, 68 tail of milter；Second of selection 82 tail of raun, 90 tail of milter；Third time selection 59 tail of raun, milter 72.
Raun of wherein hastening parturition amounts to 174 tails, and the mean spawning rate reaches 90.4 ﹪, obtains fertilized eggs 700,000 altogether, it is average by
Smart rate reaches 87 ﹪.
1. Lhasa of table is naked to split buttocks spawning rate
2. Lhasa of table is naked to split buttocks rate of fertilization
A specific embodiment of the invention above described embodiment only expresses, the description thereof is more specific and detailed, but simultaneously
Limitations on the scope of the patent of the present invention therefore cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to guarantor of the invention
Protect range.
Claims (9)
1. a kind of naked induced spawning method for splitting buttocks fish in Lhasa, comprising the following steps:
A, parent population selects
Select it is healthy and strong, physically well develop, without obvious wound and without deformity wild Lhasa it is naked split buttocks parent population, choose parent population and exist
Enter Chi Qianyong potassium permanganate to carry out disinfection, is then divided in parent population storage pond, holding pond by male and female and temporarily supports 1 day；Storage pond, holding pond uses Gao Meng in advance
Sour potassium soaking one week, is cleaned up with clear water later；The parent population storage pond, holding pond water temperature is 11~12 DEG C, and dissolved oxygen is 6~8mg/L,
PH is 7.2~7.5；
B, artificial induced spawning
Using pectoral fin base portion injection method, injection dosage are as follows: 5 μ g luteotropin releasing hormone d-ala analogs/kg+2.5mg carp brain hangs down
Body/kg+1ml fish physiological saline；
Raun is injected 2 times, for the first time injection 30%, interval second of injection 70% after 24 hours；Milter is injected 1 time, with raun
Second of injection is synchronous to be carried out, and injection dosage halves；Female milter is put into togerther glass after second of oxytocic drug injection
In the spawning pond of steel, parent population is observed every 4 hours, checks that circulation runs well, while opening water pump, carries out
20 minutes stimulation by running water；
C, artificial insemination
The injection of second oxytocic drug for 24 hours after, check artificial induced spawning parent population, inseminated using dry method；
D, artificial incubation
Fertilized eggs are put into hatching frame, micro- Lotic hatching is carried out, control water temperature is 11~12 DEG C, and dissolved oxygen is 6~8mg/L, pH
It is 7.2~7.5.
2. the naked induced spawning method for splitting buttocks fish in Lhasa according to claim 1, it is characterised in that: the proportion of male and female parent population is
1:1.2。
3. the naked induced spawning method for splitting buttocks fish in Lhasa according to claim 1, it is characterised in that: the parent population of the step A
Storage pond, holding pond is 1.2m glass reinforced plastic round tank, and the spawning pond of step B is the glass reinforced plastic circle spawning pond that diameter is 2.5m.
4. the naked induced spawning method for splitting buttocks fish in Lhasa according to claim 1, it is characterised in that: the step B is being injected
When oxytocic drug, obstacle is wrapped up above syringe needle, exposed needle length is 0.5cm.
5. the naked induced spawning method for splitting buttocks fish in Lhasa according to claim 1, it is characterised in that: the step B, flowing water
The flow velocity of stimulation is 0.1-0.2m/s.
6. the naked induced spawning method for splitting buttocks fish in Lhasa according to claim 1, it is characterised in that: the dry method, which is inseminated, is
Refer to, dry raun body surface moisture with dry towel first, movement is soft, prevents scratch, and it is 30cm's that ovum, which is squeezed into diameter,
In white ceramic bowl, milter sperm is extruded on ovum by same movement and method, is gently agitated for 10 seconds with feather, slow along ceramic bowl wall with wooden dipper
Slow plus a little clear water, has just flooded ovum preferably, has been gently agitated for 20 seconds with feather again, later plus clear water is washed three times.
7. the naked induced spawning method for splitting buttocks fish in Lhasa according to claim 1, it is characterised in that: the D step, hatching
Ovum density of putting in frame is ten thousand Li/㎡ of 4.1-4.2.
8. the naked induced spawning method for splitting buttocks fish in Lhasa according to claim 1, it is characterised in that: the miniflow of the D step
Water hatching refers to that water flow is 1.5 cubes ms/h.
9. the naked induced spawning method for splitting buttocks fish in Lhasa according to claim 1, it is characterised in that: the hatching of the D step
Frame is that mesh is the wooden hatching frame of 10 purposes.
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