CN108293924A - A kind of artificial fecundation method of Ptychobarbus dipogon - Google Patents
A kind of artificial fecundation method of Ptychobarbus dipogon Download PDFInfo
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- CN108293924A CN108293924A CN201810265262.9A CN201810265262A CN108293924A CN 108293924 A CN108293924 A CN 108293924A CN 201810265262 A CN201810265262 A CN 201810265262A CN 108293924 A CN108293924 A CN 108293924A
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- ptychobarbus
- dipogon
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses a kind of artificial fecundation methods of Ptychobarbus dipogon, include the following steps：(1) disinfection of culture pond；(2) parent fish rearing；(3) artificial induced spawning；(4) artificial insemination；(5) hatch.The present invention solves the problems, such as that wild Ptychobarbus dipogon is reduced increasingly; pass through artificial propagation; reliable basis can be provided for introduces a collection; Ptychobarbus dipogon is important one of the indigenous economic fish in Tibet; it is distributed mainly on Yarlungzangbo water system upper and middle reaches mainstream and its tributary; with important protection of resources and value of exploiting and utilizing, the present invention produce it is double must leaves palpus juvenile fish for Yarlung Zangbo River middle reaches fishery conservation with utilize it is significant.
Description
Technical field
The present invention relates to the reproduction technique of fish, especially a kind of artificial fecundation method of Ptychobarbus dipogon.
Background technology
Ptychobarbus dipogon belongs to Cypriniformes, Cyprinidae, Schizothoracinae, and Ptychobarbus is commonly called as double palpus skin-carp meats, is Tibet weight
One of indigenous economic fish wanted is distributed mainly on Yarlungzangbo water system upper and middle reaches mainstream and its tributary, is Yarlung Zangbo River leaf
It must be distributed most wide, most common kind in fish category.In recent years, with the fast development of Tibet's economy society, the mankind are to Tibet fishing
The development and utilization intensity of industry resource continues to increase.The Yarlung Tsangpo River Basin of population collection is undoubtedly also by further strong people
Class moving obstacle, Alien Fishes invasion, the construction of water power water conservancy projects, overfishing, water pollution etc..These disturbing factors
Influence to Yarlung Zangbo River ecology of water, the especially stock of fish and its living environment becomes clear day by day.162 kinds of Qinghai-Tibet Plateans
In fish, just there are 35 kinds in pole danger, in imminent danger, easy danger or the field fish that die out, be more than 20%.
In addition, Tibet is located in Qinghai-Tibet Platean, geographical environment is special, ecological environment frailty, ecosystem structure it is simple and
Plateau fish have slow-growing, sexal maturity is late, reproductive capacity is low, the resource supplement period is long, habitat height is adapted to and relied on etc.
Feature so that plateau fish are extremely sensitive to the variation of ecological environment, and resource is once destroyed, by extremely difficult recovery, and
Most of fish are all the endemic species of locality, therefore the protection carried out to the Yarlung Zangbo River stock of fish seems particularly heavy with utilization
Will with it is urgent.Ptychobarbus dipogon has been seriously damaged as the distinctive economic kind in Tibet, stock number, individual become younger and
Miniaturization is on the rise.However, at present the research in relation to Ptychobarbus dipogon it is less, focus mostly on taxology, biology, origin and
Develop and the research of chromosome number etc..Therefore, carry out the artificial propagation research of Ptychobarbus dipogon, it is peculiar for protecting this
The meaning of its Stock resoures of fish and reasonable development has some idea of.
The patent of invention of publication number CN106577389A and CN102077787A respectively disclose a kind of artificial breeding of schizothoracin
Method is grown, disclosed method includes parent population selection and four cultivation, artificial induced spawning, artificial insemination and hatching steps.But it manually awards
Precision method has specific aim, and for different fishes, ocyodinic is also different, and incubation condition has specificity, therefore above method is not
Artificial propagation suitable for Ptychobarbus dipogon.
Invention content
To solve problems of the prior art, the present invention provides a kind of artificial fecundation methods of Ptychobarbus dipogon.
The present invention proposes the artificial fecundation method suitable for Ptychobarbus dipogon, for protecting this Endemic fish and reasonable development its kind
Group's resource is of great significance.
The technical solution adopted by the present invention is：
A kind of artificial fecundation method of Ptychobarbus dipogon, includes the following steps：
(1) disinfection of culture pond：Potassium permanganate is added in the water of culture pond to impregnate 24 hours, then is cleaned and is done with clear water
Only, it is put into natural flow；
(2) parent fish rearing：Parent population, timing, quantitative, fixed point input artifical compound feed and animal are put into culture pond
Property bait；
(3) artificial induced spawning：It hastens parturition to parent population in the April, luteotropin releasing hormone d-ala analog LRH-A2And carp
Pituitary PG mixture hastens parturition to Ptychobarbus dipogon raun using biphasic injection, milter injection time and the second needle of raun
Synchronous, dosage is the one third of raun；
(4) artificial insemination：Raun is preovulatory, collects milter sperm and carries out microscopy, 4 DEG C of preservations of sperm of microscopy qualification are standby
With by 48~60 hours Effect times, after raun ovulation, using the fertilization of anhydrous dry method；
(5) hatch：Fertilized eggs are placed in hatching frame, clear water is filled with and is hatched；In hatching process, using miniflow water
Amount is managed, and fertilized eggs is kept to remain static in hatching frame；On inspection the ovum not being fertilized and death are selected out with point
Embryo；Hatching was demoulding after 7~9 days, then put down trip and become juvenile fish in 23 days.
The present invention hastens parturition to parent population in the April, uses luteotropin releasing hormone d-ala analog LRH-A2And carp brain
Hypophysis PG mixture hastens parturition to Ptychobarbus dipogon raun using biphasic injection as ocyodinic, the injection of the first needle of raun
Afterwards, it is spaced 24 hours and carries out the second needle injection.Milter is only injected once, and injection time is synchronous with the second needle of raun, and dosage is female
The one third of fish.It is hastened parturition mixture using fish, spawning effect is relatively more preferable using single ocyodinic effect, is especially embodied in fertilization
In terms of rate.
The present invention is stored refrigerated for short-term preservation sperm at 4 DEG C, can save sperm a couple of days to dozens of days, during breeding
Be conveniently operated, can with take with.Effect time is influenced by factors such as water temperature and oxytocic hormones, is bred in plateau cold water fishes
Under conditions of 10 DEG C~13 DEG C of water temperature, the Effect time using mixture of hastening parturition is shorter than single hormone, and comprehensive statistics Effect time exists
Between 48~60 hours.
Further, a concentration of 500mg/L of potassium permanganate in the step (1), the concentration are can to ensure to kill big portion
Divide the minimum concentration of pathogen.
Further, the condition of water quality of natural flow is in the step (2)：Water flow is 7200L/h, dissolved oxygen 6.5mg/
L or more, 10 DEG C~13 DEG C of water temperature.The condition of water quality is simulation Ptychobarbus dipogon natural propagation condition.
Further, quantitative feeding amount is the 2%~5% of fish body weight in the step (2).Both it can guarantee Ptychobarbus dipogon
Daily ration demand, and do not cause residual bait to remain, it not only wasted, but also pollution water quality.
Further, the weight of parent population is 500g~1000g in the step (2).
Further, the first injection amount of biphasic injection is 4 μ g/ of luteotropin releasing hormone d-ala analog in the step (3)
The μ of kg~8 g/kg and carp pituitary 2mg/kg~4mg/kg.The effect of first needle is to promote sperm and maturation of ovum.It is above-mentioned
Dosage is excessively high to damage parent population health, and concentration is too low, cannot reach effect of accelerating the ripening.
Further, the second injection amount of biphasic injection is 8 μ g/ of luteotropin releasing hormone d-ala analog in the step (3)
The μ of kg~12 g/kg and carp pituitary 4mg/kg~6mg/kg.The effect of second needle is to promote the row of mature sperm and ovum
Go out.Above-mentioned dosage is excessively high to damage parent population health, and concentration is too low, cannot reach spawning effect.
Further, the stationary state in the step (5) is fertilized eggs forbidden moves before demoulding in hatching frame.Double palpus leaves
There are sensitive periods in hatching process for the fertilized eggs of palpus fish, if strenuous vibration meeting mortality is met in sensor, therefore should be as possible
Remains stationary.
Further, the water temperature hatched in the water temperature and step (5) hastened parturition in the step (3) is 10 DEG C~13 DEG C.
The present invention proposes the artificial fecundation method suitable for Ptychobarbus dipogon, and hatching rate is up to 50%, for protecting this
One Endemic fish and reasonable development its Stock resoures are of great significance.
Specific implementation mode
In order to it is clearer, explain purpose of the present invention technical solution in detail, below by related embodiment to this hair
It is bright to be described further.Following embodiment is only to illustrate the implementation of the present invention, does not limit the protection of the present invention
Range.
Embodiment 1
A kind of artificial fecundation method of Ptychobarbus dipogon, includes the following steps：
(1) disinfection of culture pond：Potassium permanganate is added in the water of culture pond to impregnate culture pond, then uses clear water
Clean up, it is to be sterilized after after 24 hours, be put into natural flow later；
(2) parent fish rearing：Parent population is put into natural flow, timing, quantitative, fixed point input are with artifical compound feed
It is main, it is aided with yellow meal worm, fly maggot class animal bait；
(3) artificial induced spawning：It hastens parturition to parent population in the April, selects luteotropin releasing hormone d-ala analog (LRH-A2)
Hastened parturition to Ptychobarbus dipogon raun using biphasic injection with carp pituitary (PG) mixture, milter injection time with it is female
The second needle of fish synchronizes, and dosage is the one third of raun；
(4) artificial insemination：Raun is preovulatory, collects milter sperm and carries out microscopy, 4 DEG C of preservations of sperm of microscopy qualification are standby
With by 48 hours Effect times, after raun ovulation, using the fertilization of anhydrous dry method；
(5) hatch：Fertilized eggs are placed in hatching frame, clear water is filled with and is hatched；In hatching process, using miniflow water
Amount is managed, and fertilized eggs is kept to remain static in hatching frame；
Running check and point select out the ovum not being fertilized and dead embryo；
Hatching was demoulding after 7 days, then put down trip and become juvenile fish in 23 days.
Embodiment 2
A kind of artificial fecundation method of Ptychobarbus dipogon, includes the following steps：
(1) disinfection of culture pond：Water level 20cm~30cm is reserved in culture pond, is added in the water of culture pond later
Potassium permanganate impregnates culture pond, then is cleaned up with clear water, it is to be sterilized after after 24 hours, be put into natural flow later；
(2) parent fish rearing：Parent population is put into natural flow, timing, quantitative, fixed point input are with artifical compound feed
It is main, it is aided with yellow meal worm, fly maggot class animal bait；
(3) artificial induced spawning：It hastens parturition to parent population in the April, selects luteotropin releasing hormone d-ala analog (LRH-A2)
Hastened parturition to Ptychobarbus dipogon raun using biphasic injection with carp pituitary (PG) mixture, milter injection time with it is female
The second needle of fish synchronizes, and dosage is the one third of raun；
(4) artificial insemination：Raun is preovulatory, collects milter sperm and carries out microscopy, 4 DEG C of preservations of sperm of microscopy qualification are standby
With by 60 hours Effect times, after raun ovulation, using the fertilization of anhydrous dry method；
(5) hatch：Fertilized eggs are placed in hatching frame, clear water is filled with and is hatched；In hatching process, using miniflow water
Amount is managed, and fertilized eggs is kept to remain static in hatching frame；
Running check and point select out the ovum not being fertilized and dead embryo；
Hatching was demoulding after 9 days, then put down trip and become juvenile fish in 23 days.
A concentration of 500mg/L of potassium permanganate in the step (1).
Quantitative feeding amount is the 2% of fish body weight in the step (2).
The weight of parent population is 500g in the step (2).
The water temperature hatched in the water temperature and step (5) hastened parturition in the step (3) is 10 DEG C.
Embodiment 3
A kind of artificial fecundation method of Ptychobarbus dipogon, includes the following steps：
(1) disinfection of culture pond：Potassium permanganate is added in the water of culture pond to impregnate culture pond, then uses clear water
Clean up, it is to be sterilized after after 24 hours, be put into natural flow later；
(2) parent fish rearing：Parent population is put into natural flow, timing, quantitative, fixed point input are with artifical compound feed
It is main, it is aided with yellow meal worm, fly maggot class animal bait；
(3) artificial induced spawning：It hastens parturition to parent population in the April, selects luteotropin releasing hormone d-ala analog (LRH-A2)
Hastened parturition to Ptychobarbus dipogon raun using biphasic injection with carp pituitary (PG) mixture, milter injection time with it is female
The second needle of fish synchronizes, and dosage is the one third of raun；
(4) artificial insemination：Raun is preovulatory, collects milter sperm and carries out microscopy, 4 DEG C of preservations of sperm of microscopy qualification are standby
With by 55 hours Effect times, after raun ovulation, using the fertilization of anhydrous dry method；
(5) hatch：Fertilized eggs are placed in hatching frame, clear water is filled with and is hatched；In hatching process, using miniflow water
Amount is managed, and fertilized eggs is kept to remain static in hatching frame；
Running check and point select out the ovum not being fertilized and dead embryo；
Hatching was demoulding after 8 days, then put down trip and become juvenile fish in 23 days.
Quantitative feeding amount is the 5% of fish body weight in the step (2).
The weight of parent population is 1000g in the step (2).
Stationary state in the step (5) is fertilized eggs forbidden moves before hatching frame kind demoulding.
The water temperature hatched in the water temperature and step (5) hastened parturition in the step (3) is 13 DEG C.
Embodiment 4
A kind of artificial fecundation method of Ptychobarbus dipogon, includes the following steps：
(1) disinfection of culture pond：Potassium permanganate is added in the water of culture pond to impregnate culture pond, then uses clear water
Clean up, it is to be sterilized after after 24 hours, be put into natural flow later；
(2) parent fish rearing：Parent population is put into natural flow, timing, quantitative, fixed point input are with artifical compound feed
It is main, it is aided with yellow meal worm, fly maggot class animal bait；
(3) artificial induced spawning：It hastens parturition to parent population in the April, selects luteotropin releasing hormone d-ala analog (LRH-A2)
Hastened parturition to Ptychobarbus dipogon raun using biphasic injection with carp pituitary (PG) mixture, milter injection time with it is female
The second needle of fish synchronizes, and dosage is the one third of raun；
(4) artificial insemination：Raun is preovulatory, collects milter sperm and carries out microscopy, 4 DEG C of preservations of sperm of microscopy qualification are standby
With by 55 hours Effect times, after raun ovulation, using the fertilization of anhydrous dry method；
(5) hatch：Fertilized eggs are placed in hatching frame, clear water is filled with and is hatched；In hatching process, using miniflow water
Amount is managed, and fertilized eggs is kept to remain static in hatching frame；
Running check and point select out the ovum not being fertilized and dead embryo；
Hatching was demoulding after 8 days, then put down trip and become juvenile fish in 23 days.
Quantitative feeding amount is the 3% of fish body weight in the step (2).
The weight of parent population is 800g in the step (2).
Stationary state in the step (5) is fertilized eggs forbidden moves before hatching frame kind demoulding.
The water temperature hatched in the water temperature and step (5) hastened parturition in the step (3) is 11 DEG C.
Embodiment 5
On the basis of embodiment 1, the condition of water quality of natural flow is the present embodiment in the step (2)：Water flow is
700L/h, dissolved oxygen 7mg/L, 10 DEG C of water temperature.
Embodiment 6
On the basis of embodiment 2, the condition of water quality of natural flow is the present embodiment in the step (2)：Water flow is
700L/h, dissolved oxygen 8mg/L, 13 DEG C of water temperature.
Embodiment 7
On the basis of embodiment 3, the condition of water quality of natural flow is the present embodiment in the step (2)：Water flow is
700L/h, dissolved oxygen 10mg/L, 12 DEG C of water temperature.
Embodiment 8
For the present embodiment on the basis of embodiment 1, the first injection amount of biphasic injection is luteotropin in the step (3)
Releasing hormone analog 4 μ g/kg and carp pituitary 2mg/kg.
Embodiment 9
For the present embodiment on the basis of embodiment 2, the first injection amount of biphasic injection is luteotropin in the step (3)
Releasing hormone analog 8 μ g/kg and carp pituitary 4mg/kg.
Embodiment 10
For the present embodiment on the basis of embodiment 3, the first injection amount of biphasic injection is luteotropin in the step (3)
Releasing hormone analog 6 μ g/kg and carp pituitary 3mg/kg.
Embodiment 11
For the present embodiment on the basis of embodiment 1, the second injection amount of biphasic injection is luteotropin in the step (3)
Releasing hormone analog 8 μ g and carp pituitary 4mg/kg.
Embodiment 12
For the present embodiment on the basis of embodiment 2, the second injection amount of biphasic injection is luteotropin in the step (3)
Releasing hormone analog 12 μ g/kg and carp pituitary 6mg/kg.
Embodiment 13
For the present embodiment on the basis of embodiment 3, the second injection amount of biphasic injection is luteotropin in the step (3)
Releasing hormone analog 10 μ g/kg and carp pituitary 5mg/kg.
Specific implementation mode of the invention above described embodiment only expresses, the description thereof is more specific and detailed, but simultaneously
Cannot the limitation to the scope of the claims of the present invention therefore be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the guarantor of the present invention
Protect range.
Claims (9)
1. a kind of artificial fecundation method of Ptychobarbus dipogon, which is characterized in that include the following steps：
(1) disinfection of culture pond：Potassium permanganate is added in the water of culture pond to impregnate 24 hours, then is cleaned up with clear water,
It is put into natural flow；
(2) parent fish rearing：Parent population, timing, quantitative, fixed point input artifical compound feed and animality bait are put into culture pond
Material；
(3) artificial induced spawning：It hastens parturition to parent population in the April, luteotropin releasing hormone d-ala analog LRH-A2It hangs down with carp brain
Body PG mixture hastens parturition to Ptychobarbus dipogon raun using biphasic injection, and milter injection time and the second needle of raun are same
Step, dosage are the one third of raun；
(4) artificial insemination：Raun is preovulatory, collects milter sperm and carries out microscopy, and 4 DEG C of the sperm of microscopy qualification saves backup, and passes through
48~60 hours Effect times are crossed to be fertilized using anhydrous dry method after raun ovulation；
(5) hatch：Fertilized eggs are placed in hatching frame, clear water is filled with and is hatched；In hatching process, using miniflow water into
Row management keeps fertilized eggs to remain static in hatching frame；On inspection the ovum not being fertilized and dead embryo are selected out with point；
Hatching was demoulding after 7~9 days, then put down trip and become juvenile fish in 23 days.
2. a kind of artificial fecundation method of Ptychobarbus dipogon according to claim 1, which is characterized in that the step (1)
In a concentration of 500mg/L of potassium permanganate.
3. a kind of artificial fecundation method of Ptychobarbus dipogon according to claim 1, which is characterized in that the step (2)
In the condition of water quality of natural flow be：Water flow is 700L/h, and dissolved oxygen is 6.5mg/L or more, 10 DEG C~13 DEG C of water temperature.
4. a kind of artificial fecundation method of Ptychobarbus dipogon according to claim 1, which is characterized in that the step (2)
In quantitative feeding amount be the 2%~5% of fish body weight.
5. a kind of artificial fecundation method of Ptychobarbus dipogon according to claim 1, which is characterized in that the step (3)
First injection amount of middle biphasic injection is 4 μ of μ g/kg~8 g/kg of luteotropin releasing hormone d-ala analog and carp pituitary
2mg/kg~4mg/kg.
6. a kind of artificial fecundation method of Ptychobarbus dipogon according to claim 1, which is characterized in that the step (3)
Second injection amount of middle biphasic injection is 8 μ of μ g/kg~12 g/kg of luteotropin releasing hormone d-ala analog and carp pituitary
4mg/kg~6mg/kg.
7. a kind of artificial fecundation method of Ptychobarbus dipogon according to claim 1, which is characterized in that the step (5)
In stationary state refer to fertilized eggs forbidden moves before demoulding in hatching frame.
8. a kind of artificial fecundation method of Ptychobarbus dipogon according to claim 1, which is characterized in that the step (3)
In the water temperature hastened parturition and the water temperature hatched in step (5) be 10 DEG C~13 DEG C.
9. a kind of artificial fecundation method of Ptychobarbus dipogon according to claim 1, which is characterized in that the miniflow flow
Amount is 700L/h.
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| CN112655847A (en) * | 2020-12-30 | 2021-04-16 | 西藏自治区农牧科学院水产科学研究所 | Food calling feed additive and feed for Leptospira bifidus |
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| CN112293316A (en) * | 2020-11-13 | 2021-02-02 | 西藏自治区农牧科学院水产科学研究所 | Artificial large-scale hatching method and system for Tibet double-whisker-leaf-whisker-fish eggs |
| CN112293316B (en) * | 2020-11-13 | 2022-06-17 | 西藏自治区农牧科学院水产科学研究所 | Artificial large-scale hatching method and system for Tibet double-whisker spiraea sinensis roes |
| CN112655847A (en) * | 2020-12-30 | 2021-04-16 | 西藏自治区农牧科学院水产科学研究所 | Food calling feed additive and feed for Leptospira bifidus |
| CN113331087A (en) * | 2021-06-10 | 2021-09-03 | 武汉中科瑞华生态科技股份有限公司 | Artificial propagation method of Gymnocypris ventricosa |
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