CN109644902A - A kind of Lhasa schizothoracin artificial breeding method - Google Patents
A kind of Lhasa schizothoracin artificial breeding method Download PDFInfo
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- CN109644902A CN109644902A CN201811601835.7A CN201811601835A CN109644902A CN 109644902 A CN109644902 A CN 109644902A CN 201811601835 A CN201811601835 A CN 201811601835A CN 109644902 A CN109644902 A CN 109644902A
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- lhasa
- schizothoracin
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- 241000252206 Cypriniformes Species 0.000 description 2
- 108700012941 GNRH1 Proteins 0.000 description 2
- 239000000579 Gonadotropin-Releasing Hormone Substances 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- KIPLYOUQVMMOHB-MXWBXKMOSA-L [Ca++].CN(C)[C@H]1[C@@H]2[C@@H](O)[C@H]3C(=C([O-])[C@]2(O)C(=O)C(C(N)=O)=C1O)C(=O)c1c(O)cccc1[C@@]3(C)O.CN(C)[C@H]1[C@@H]2[C@@H](O)[C@H]3C(=C([O-])[C@]2(O)C(=O)C(C(N)=O)=C1O)C(=O)c1c(O)cccc1[C@@]3(C)O Chemical compound [Ca++].CN(C)[C@H]1[C@@H]2[C@@H](O)[C@H]3C(=C([O-])[C@]2(O)C(=O)C(C(N)=O)=C1O)C(=O)c1c(O)cccc1[C@@]3(C)O.CN(C)[C@H]1[C@@H]2[C@@H](O)[C@H]3C(=C([O-])[C@]2(O)C(=O)C(C(N)=O)=C1O)C(=O)c1c(O)cccc1[C@@]3(C)O KIPLYOUQVMMOHB-MXWBXKMOSA-L 0.000 description 2
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- 208000036119 Frailty Diseases 0.000 description 1
- 241001540526 Leptonotus elevatus Species 0.000 description 1
- AFVFQIVMOAPDHO-UHFFFAOYSA-N Methanesulfonic acid Chemical compound CS(O)(=O)=O AFVFQIVMOAPDHO-UHFFFAOYSA-N 0.000 description 1
- 241001275867 Schizothorax Species 0.000 description 1
- 241000066375 Schizothorax waltoni Species 0.000 description 1
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- 206010003549 asthenia Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 210000001136 chorion Anatomy 0.000 description 1
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- KQTVWCSONPJJPE-UHFFFAOYSA-N etridiazole Chemical compound CCOC1=NC(C(Cl)(Cl)Cl)=NS1 KQTVWCSONPJJPE-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The present invention provides a kind of Lhasa schizothoracin artificial breeding method, include the following steps: that parent population must be bred after 1) raising and train wild Lhasa schizothoracin；2) it is divided to the two needles oxytocic hormone that injection is made of human chorionic gonadtropin, luteotropin releasing hormone d-ala analog and dioxone to carry out artificial induced spawning breeding parent population, obtains fertilized eggs；3) artificial incubation is carried out to fertilized eggs using 10~15 DEG C of miniflow water, until hatching emergence, obtains Lhasa schizothoracin seedling；4) Lhasa schizothoracin seedling is put in a suitable place to breed to pond, hatching emergence starts to feed food after 10 days, until growing up to obtain Lhasa schizothoracin.The invention injects oxytocic hormone by the way of being divided to two needles to inject; and the every needle injection amount of strict control; it can obtain well-developed ovum and sperm; incubating oosperm condition is controlled simultaneously; so that 75% or more Lhasa schizothoracin spawning rate, 90% or more rate of fertilization; 85% or more hatching rate realizes the large-scale artificial breeding of Lhasa schizothoracin, the effective protection resource.
Description
Technical field
The invention belongs to fish culture technical fields, and in particular to a kind of Lhasa schizothoracin artificial breeding method.
Background technique
Lhasa schizothoracin (Schizothorax waltoni Regan) is subordinate to Cypriniformes (Cypriniformes) Cyprinidae
(Cyprinidae) Schizothoracinae (Schizothoracinae) Schizothorax (SchizothoraxHeckel), alias Lhasa
Bend fish, bayesian schizothoracin, be commonly called as pipefish, be distributed in each Heavenly Stems and Earthly Branches stream in Yarlung Zangbo River Milin or more to Rikaze, is China
Endemic species, one of Tibet region Main Commercial Fishes have important researching value.
In recent decades, with the fast development of Tibet's economy society, development and utilization of the mankind to Tibet fishery resources
Intensity continues to increase.The Yarlung Tsangpo River Basin of population collection is undoubtedly also interfered by further strong mankind's activity, such as outer
Come fish invasion, the construction of water power water conservancy projects, overfishing, water pollution etc., these disturbing factors are to Yarlung Zangbo River waters
The influence of ecology, the especially stock of fish and its living environment becomes clear day by day.In 162 kinds of Qinghai-Tibet fish, endanger in pole,
In imminent danger, easy danger or the field fish that die out just have 35 kinds, have been more than 20%, in Yarlung Zangbo River middle reaches, six kinds of common schizothoracins
In class, it just is in Critical Condition there are three types of fish, wherein just including Lhasa schizothoracin.In addition, Tibet is located in Qinghai-Tibet Platean, geographical
Environment is special, ecological environment frailty, and ecosystem structure is simple and plateau fish have slow growth, sexal maturity evening, breeding
Power is low, the resource supplement period is long, the features such as habitat height is adapted to and relied on, so that variation pole of the plateau fish to ecological environment
For sensitivity, resource is once destroyed, and by extremely difficult recovery, and most of fish are all local endemic species, therefore to refined
The river the Lu Zangbu stock of fish, especially schizothoracin class resource take certain safeguard measure be particularly important with it is urgent.
Currently, part research worker has paid effort to Lhasa schizothoracin raising technology, but raising technology is not very
Stablize, germ plasm resource conservation and marketing also have certain risk, and there are still bottleneck problems, such as Lhasa for part raising technology
During the fertilization of schizothoracin artificial induced spawning and incubating oosperm, spawning rate, rate of fertilization and hatching rate are very low, and emergence rate generally exists
30% or so, it is difficult to realize the scale supply of Lhasa schizothoracin seed.
Summary of the invention
It, can not the purpose of the present invention is overcoming existing Lhasa schizothoracin artificial breeding spawning rate, rate of fertilization and hatching rate low
The problem of realizing large-scale artificial breeding.
For this purpose, including the following steps: the present invention provides a kind of Lhasa schizothoracin artificial breeding method
1) preparation of parent population is bred
The wild Lhasa schizothoracin of fishing is transported to raising and train in pond, is uniformly mixed using water earthworm and Salmons feed
It carrying out taming and dociling food, every day feeding volume is the 2~6% of fish body weight, it feeds daily 2 times, until wild Lhasa schizothoracin starts feed of ingesting,
It tames successfully, it is spare as breeding parent population；
2) artificial induced spawning is fertilized
Breeding parent population in step 1) is anaesthetized, the injection of two needles is divided to be released by human chorionic gonadtropin, luteotropin
The oxytocic hormone for putting hormone analogs and dioxone composition carries out artificial induced spawning, and two needle injection intervals 24 hours obtain developing just
Normal ovum and sperm, then dry method fertilization is carried out, obtain fertilized eggs；
Wherein, for breeding parent population in raun, the first needle injection oxytocic hormone in human chorionic gonadtropin 300~
500IU/kg fish body weight, luteotropin releasing hormone d-ala analog 2~5ug/kg fish body weight, dioxone 2~3ug/kg fish body weight, the
Human chorionic gonadtropin 800~1000IU/kg fish body weight in the oxytocic hormone of two needles injection, luteinizing hormone releasing hormone are similar
4~7 μ g/kg fish body weight of object, 5~6 μ g/kg fish body weight of dioxone；It is raun for two needle injection amount of milter in breeding parent population
The half of injection dosage；
3) artificial incubation
Artificial incubation is carried out to the fertilized eggs that step 2) obtains using 10~15 DEG C of miniflow water, until hatching emergence, obtains
Lhasa schizothoracin seedling；
4) seedling culture
The Lhasa schizothoracin seedling that step 3) hatches to be put in a suitable place to breed to pond, hatching emergence starts to feed food after 10 days,
To growing up to obtain Lhasa schizothoracin.
Further, described to raise and train in pond using dissolved oxygen not less than 6mg/L, pH value 7.8~8.4,10~15 DEG C of water temperature
Mountain stream water, and 24 hours flowing water changes.
Further, gill net is taken in the step 1), is casted net and is split abdomen with the wild Lhasa of micro-current fishing style fishing
Fish, and the wild Lhasa schizothoracin of injury caught smears wound using terramycin ointment, erythromycin ointment or salt, or wild
Penicillin is injected intraperitoneally in Lhasa schizothoracin.
Further, the wild Lhasa schizothoracin first Balance Water Quality water temperature before entering to raise and train pond, makes to transport wild drawing
The water temperature of Sa schizothoracin device and raise and train water temperature unanimously；After wild Lhasa schizothoracin enters to raise and train pond, not feeding in 3 days, and
Raise and train vitamin C of splashing in pond.
Further, the MS-222 anesthesia liquid of 100mg/L is used in the step 2) to breeding parent population anesthesia.
Further, the process of micro- Lotic hatching fertilized eggs is hatching frame to be put into hatching cylinder in the step 3), is added
Fertilized eggs are put into after water, it is 3~5 fertilized eggs/cm that each hatching frame, which is put into density,2Hatched, hatches frame depth of water 10cm.
Further, the pond of the seedling culture uses the streams water through precipitating aeration.
Further, schizothoracin seedling breeding density in Lhasa is every square metre 1000~1500 of spray seedling in the step 4)
Tail, long 1.5 centimetres of 800~1000 tail of every square metre of seedling of body；Lhasa schizothoracin seedling put in a suitable place to breed the time be the morning 8~9 when or
When afternoon 16~17.
Further, feeding cooking cycle in the step 4) after hatching emergence is to hatch 10 days, opening feed, opening food
Object feeds cooked egg yolk, and egg yolk is fed 6 times daily；Opening starts to feed micro-encapsulated diet S1 after 7 days, feeds 5 daily
It is secondary；Opening feeds micro-encapsulated diet S1 and fairy shrimp after 15 days, opening uses micro-encapsulated diet S1 and micro-encapsulated diet after 20 days
S2 feedstuff feeding is fed 4 times daily.
Compared with prior art, beneficial effects of the present invention:
This Lhasa schizothoracin artificial breeding method provided by the invention using be divided to two needles inject by the way of inject hasten parturition it is sharp
Element, and the every needle injection amount of strict control, can obtain well-developed ovum and sperm, improve Lhasa schizothoracin spawning rate and
Rate of fertilization；Fertilized eggs are hatched using micro- pipeline mode simultaneously, both can guarantee the discharge condition of incubating oosperm, and can protect
The water surface is static under card discharge condition, and dead ovum phenomenon, improves the hatching rate of fertilized eggs caused by avoiding fertilized eggs mobile.
Specific embodiment
The following is a clear and complete description of the technical scheme in the embodiments of the invention, it is clear that described embodiment
Only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, the common skill in this field
Art personnel all other embodiment obtained without making creative work belongs to the model that the present invention protects
It encloses.
A kind of Lhasa schizothoracin artificial breeding method is present embodiments provided, is included the following steps:
(1) preparation of parent population is bred
The wild Lhasa schizothoracin of fishing is transported to raising and train in pond, using water earthworm and Salmons feed (Chengdu lattice
Lan company production, specification be 0.5# and 0.8# Salmons feed, protein content 58%) be uniformly mixed carry out tame and docile food, often
Day feeding volume is the 2~6% of fish body weight, feeds 2 times, until wild Lhasa schizothoracin starts feed of ingesting, that is, tames successfully daily,
It is spare as breeding parent population.
Specifically, wild Lhasa schizothoracin takes gill net during fishing, casts net and fish with modes such as micro-current fishings；
And the fish collected is started shipment after temporarily supporting to improve transportation survival rate, injured fish use in time terramycin ointment, erythromycin ointment,
Salt etc. smears wound, and penicillin is injected intraperitoneally in part fish.
The transport for breeding parent population can select different transports according to the distance and convenient, parent population specification and quantity of distance
Mode.One is live fish car transports, are transported using big iron sheet case, are mainly used for for wild fish being transported to and temporarily increase soil fertility, the built-in Qing Dynasty is clean
Water, gas tube is installed, external oxygen increasing equipment by gas tube inflate by the water body into case, guarantees that dissolved oxygen fills in water body in case
Foot；It is no more than 60 kilograms per cubic meter with the density that live fish car transports breeding parent population, each haulage time is no more than 24 hours.Separately
One is oxygen bag transports, are mainly used for wild parent population in situ conservation, and wild parent population is packed into the oxygen bag being filled with water, and are oxygenated, will
Oxygen bag is put bubble chamber into and is packaged, then bubble chamber is put into carton, and packaging carton can transport.
And since wild Lhasa schizothoracin is after fishing and transport, there is certain stress reaction, thus, optimization, it is wild
Raw Lhasa schizothoracin needs Balance Water Quality water temperature, its adaptation is waited to raise and train pool environment about 30 minutes after transport is reached and raised and train on pool side,
Transport the water temperature of wild Lhasa schizothoracin device (live fish car or oxygen bag) with raise and train water temperature it is consistent after fish is slowly poured into again
It raises and train in pond, it is possible to reduce because dead caused by transport stress and water temperature change dramatically.Further, fish enters raise and train pond after, 3 days
Inside not feeding, full pool spilling head vitamin C, reduction stress, while paying attention to observing.
In addition, wild Lhasa schizothoracin raises and train pond and is disappeared with the potassium permanganate that concentration is 15mg/L before being thrown to and raising and train pond
Poison.The 10m that is about in pond is raised and train, 4 meters wide, a height of 1.50m, the depth of water is 1m, substrate pan soil or cement in pond, cement plate slope protection,
Chi Zhongshui is raised and train using dissolved oxygen not less than 6mg/L, pH value 7.8~8.4,10~15 DEG C of water temperature of mountain stream water, and 24 hours flow
Move water, 10~15 tails of breeding density/m2.Wild Lhasa schizothoracin in raising and train pond using tame and docile food scheme as shown in Table 1 into
Row is raised and train, and wild Lhasa schizothoracin can start ingest feed (i.e. Salmons feed) after raising and train, and tame successfully, Ji Kezuo
To breed parent population.
Table 1
(2) artificial induced spawning is fertilized
The apparent milter of head pearl star and the soft and elastic raun of abdomen, are urging in the breeding parent population of screening step (1)
It is antenatal that first breeding parent population is anaesthetized using MS-222 (gavaculine ethyl ester mesylate) anesthesia liquid of 100mg/L,
Avoid the breeding subsequent fertilization of hastening parturition of parent population from water manipulation process because stress reaction leads to that premunition decline, survival rate is low asks
Topic.
When carrying out artificial induced spawning to postanesthetic breeding parent population, using by human chorionic gonadtropin (HCG), rush corpus luteum
The oxytocic hormone that hormone-releasing hormone analog (LRH-A) and dioxone are constituted is divided to two needles to inject, and two needle injection intervals 24 hours obtain
To normotrophic ovum and sperm, then dry method fertilization is carried out, obtains fertilized eggs.Wherein, for raun in breeding parent population, the first needle
300~500IU/kg of human chorionic gonadtropin fish body weight in the oxytocic hormone of injection, luteotropin releasing hormone d-ala analog 2~
5ug/kg fish body weight, dioxone 2~3ug/kg fish body weight, the second needle injection oxytocic hormone in human chorionic gonadtropin 800
~1000IU/kg fish body weight, 4~7 μ g/kg fish body weight of luteotropin releasing hormone d-ala analog, 5~6 μ g/kg fish body of dioxone
Weight；It is the half of raun injection dosage for two needle injection amount of milter in breeding parent population.
(3) artificial incubation
Artificial incubation is carried out to the fertilized eggs that step (2) obtains using 10~15 DEG C of miniflow water, until hatching emergence, obtains
Lhasa schizothoracin seedling.
Specifically, hatching frame is put into hatching cylinder, fertilized eggs are put into after adding water, it is 3 that density is put into each hatching frame
~5 fertilized eggs/cm2Hatched, hatch frame depth of water 10cm, under the conditions of 12~15 DEG C, hatches 221~242 hours membranes.
There is bubble illness if hatching gas supersaturation in water and will lead to the Lhasa schizothoracin seedling hatched, optimization, it can
Water is changed Spawning water in time and in water splashing 2ppm salt water.
(4) seedling culture
The Lhasa schizothoracin seedling that step (3) hatches to be put in a suitable place to breed to pond, hatching emergence starts to feed food after 10 days,
To growing up to obtain Lhasa schizothoracin.Optimization, the streams water after precipitating aeration is used with water in pond.
Wherein, spray seedling lesser for the specification hatched is put in a suitable place to breed with the density of every square metre of 1000~1500 tails, to
Start a point Chi Peiyu when 3 centimetres of aquation Miao Changzhi body length or more；1.5 centimetres of seedling long for the body hatched is with every square metre
The density of 800~1000 tails is put in a suitable place to breed.The Lhasa schizothoracin seedling put in a suitable place to breed the time be the morning 8~9 when or be preferred when afternoon 16~17.
In Lhasa, schizothoracin seedling is hatched about 10 days, and opening feed, opening food feeds cooked egg yolk, and egg yolk is every
Day feeds 6 times；Opening starts to feed micro-encapsulated diet S1 after 7 days, feeds daily 5 times；Opening fed micro-encapsulated diet after 15 days
S1 and fairy shrimp, opening, using micro-encapsulated diet S1 and micro-encapsulated diet S2 feedstuff feeding, are fed 4 times daily after 20 days.
75% or more the Lhasa schizothoracin spawning rate that is obtained using above-mentioned Lhasa schizothoracin artificial breeding method, rate of fertilization
90% or more, 85% or more hatching rate.
In addition, for artificial induced spawning in above-mentioned Lhasa schizothoracin artificial breeding method oxytocic hormone injection dosage to hastening parturition
Rate and the influence of parent population survival rate are investigated, and the results are shown in Table 2.
Table 2:
As shown in Table 2, raun uses injection dosage in the present embodiment during artificial induced spawning, i.e. the first needle injects chorion
Promoting sexual gland hormone 300~500IU/kg fish body weight, luteotropin releasing hormone d-ala analog 2~5ug/kg fish body weight, dioxone 2~
3ug/kg fish body weight, the second needle inject 800~1000IU/kg of human chorionic gonadtropin fish body weight, luteinizing hormone releasing hormone
4~7 μ g/kg fish body weight of analog, spawning rate can reach 70% or more when 5~6 μ g/kg fish body weight of dioxone, parent population survival rate
Reach 95% or more, and when injection dosage is too low substantially reduces spawning rate, spawning rate and parent population survival rate when injection dosage is excessively high
It is lower.
The foregoing examples are only illustrative of the present invention, does not constitute the limitation to protection scope of the present invention, all
It is within being all belonged to the scope of protection of the present invention with the same or similar design of the present invention.
Claims (9)
1. a kind of Lhasa schizothoracin artificial breeding method, characterized by the following steps:
1) preparation of parent population is bred
The wild Lhasa schizothoracin of fishing is transported to raising and train in pond, is uniformly mixed and is carried out using water earthworm and Salmons feed
Food is tamed and dociled, every day feeding volume is the 2~6% of fish body weight, is fed daily 2 times, until wild Lhasa schizothoracin starts feed of ingesting, that is, is tamed and dociled
It is melted into function, it is spare as breeding parent population；
2) artificial induced spawning is fertilized
Breeding parent population in step 1) is anaesthetized, is divided to the injection of two needles to be discharged by human chorionic gonadtropin, luteotropin and swashs
The oxytocic hormone that plain analog and dioxone are constituted carries out artificial induced spawning, and two needle injection intervals 24 hours obtain normotrophic
Ovum and sperm, then dry method fertilization is carried out, obtain fertilized eggs；
Wherein, for raun in breeding parent population, 300~500IU/ of human chorionic gonadtropin in the oxytocic hormone that the first needle is injected
Kg fish body weight, luteotropin releasing hormone d-ala analog 2~5ug/kg fish body weight, dioxone 2~3ug/kg fish body weight, the second needle note
800~1000IU/kg of human chorionic gonadtropin fish body weight in the oxytocic hormone penetrated, luteotropin releasing hormone d-ala analog 4~7
μ g/kg fish body weight, 5~6 μ g/kg fish body weight of dioxone；It is raun injection for two needle injection amount of milter in breeding parent population
The half of amount；
3) artificial incubation
Artificial incubation is carried out to the fertilized eggs that step 2) obtains using 10~15 DEG C of miniflow water, until hatching emergence, obtains Lhasa
Schizothoracin seedling；
4) seedling culture
The Lhasa schizothoracin seedling that step 3) hatches is put in a suitable place to breed to pond, hatching emergence starts to feed food after 10 days, until long
At obtaining Lhasa schizothoracin.
2. schizothoracin artificial breeding method in Lhasa as described in claim 1, it is characterised in that: described to raise and train in pond using dissolution
Oxygen is not less than 6mg/L, pH value 7.8~8.4,10~15 DEG C of water temperature of mountain stream water, and 24 hours flowing water changes.
3. schizothoracin artificial breeding method in Lhasa as described in claim 1, it is characterised in that: take thorn in the step 1)
Net, the wild Lhasa schizothoracin casted net with the fishing of micro-current fishing style, and the wild Lhasa schizothoracin of injury caught is using soil
Mycin ointment, erythromycin ointment or salt smear wound, or penicillin is injected intraperitoneally in wild Lhasa schizothoracin.
4. schizothoracin artificial breeding method in Lhasa as described in claim 1, it is characterised in that: the wild Lhasa schizothoracin exists
First Balance Water Quality water temperature before entering to raise and train pond makes the water temperature for transporting wild Lhasa schizothoracin device and raises and train water temperature unanimously；?
Wild Lhasa schizothoracin enters raise and train pond after, not feeding in 3 days, and raise and train vitamin C of splashing in pond.
5. schizothoracin artificial breeding method in Lhasa as described in claim 1, it is characterised in that: to breeding parent in the step 2)
Fish anesthesia uses the MS-222 anesthesia liquid of 100mg/L.
6. schizothoracin artificial breeding method in Lhasa as described in claim 1, it is characterised in that: miniflow water is incubated in the step 3)
The process for changing fertilized eggs is hatching frame to be put into hatching cylinder, adds and is put into fertilized eggs after water, each hatching frame be put into density for 3~
5 fertilized eggs/cm2Hatched, hatches frame depth of water 10cm.
7. schizothoracin artificial breeding method in Lhasa as described in claim 1, it is characterised in that: adopt in the pond of the seedling culture
With the streams water through precipitating aeration.
8. schizothoracin artificial breeding method in Lhasa as described in claim 1, it is characterised in that: abdomen is split in Lhasa in the step 4)
Fish seedling breeding density is every square metre of 1000~1500 tails of spray seedling, long 1.5 centimetres of every square metre of the seedling 800~1000 of body
Tail；Lhasa schizothoracin seedling put in a suitable place to breed the time be the morning 8~9 when or when afternoon 16~17.
9. schizothoracin artificial breeding method in Lhasa as described in claim 1, it is characterised in that: hatching emergence in the step 4)
After feed cooking cycle be hatch 10 days, opening feed, opening food feed cooked egg yolk, egg yolk is fed 6 times daily；
Opening starts to feed micro-encapsulated diet S1 after 7 days, feeds daily 5 times；Opening fed micro-encapsulated diet S1 and fairy shrimp after 15 days,
Opening, using micro-encapsulated diet S1 and micro-encapsulated diet S2 feedstuff feeding, is fed 4 times daily after 20 days.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110235818A (en) * | 2019-07-10 | 2019-09-17 | 武汉中科瑞华生态科技股份有限公司 | A kind of breeding method of preceding fin Triplophysa seed |
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Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1561704A (en) * | 2004-03-31 | 2005-01-12 | 马直科 | Artificial breeding method for schizothorax |
| CN102599095A (en) * | 2012-04-12 | 2012-07-25 | 玉龙县龙泉水产养殖科技有限责任公司 | Estrus and spawning induction method for increasing rate of artificial insemination of Schizothorax davidi (Sauvage) |
| CN103734044A (en) * | 2013-12-18 | 2014-04-23 | 华中农业大学 | Parturition hastening method for plateau-fish schizothorax oconnori |
| CN104472400A (en) * | 2014-11-11 | 2015-04-01 | 重庆市大足区炫天养殖有限责任公司 | Artificial breeding technology for schizothorax grahami |
| CN105409845A (en) * | 2015-12-01 | 2016-03-23 | 中国科学院昆明动物研究所 | Artificial reproduction method for schizothorax wangchiachii |
| CN106069927A (en) * | 2016-06-24 | 2016-11-09 | 西藏自治区农牧科学院 | A kind of domesticating method of wild Lhasa schizothoracin |
| CN107593536A (en) * | 2017-11-13 | 2018-01-19 | 张荣富 | A kind of schizothoracin breeds cultural method |
| CN108283152A (en) * | 2018-03-28 | 2018-07-17 | 武汉中科瑞华生态科技股份有限公司 | A kind of long filament schizothoracin artificial fecundation method |
| CN108324708A (en) * | 2018-03-29 | 2018-07-27 | 中国水产科学研究院长江水产研究所 | A kind of Lhasa schizothoracin anesthesia |
| CN208144206U (en) * | 2018-03-28 | 2018-11-27 | 西藏自治区农牧科学院水产科学研究所 | A kind of Lhasa schizothoracin incubating oosperm device |
-
2018
- 2018-12-26 CN CN201811601835.7A patent/CN109644902B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1561704A (en) * | 2004-03-31 | 2005-01-12 | 马直科 | Artificial breeding method for schizothorax |
| CN102599095A (en) * | 2012-04-12 | 2012-07-25 | 玉龙县龙泉水产养殖科技有限责任公司 | Estrus and spawning induction method for increasing rate of artificial insemination of Schizothorax davidi (Sauvage) |
| CN103734044A (en) * | 2013-12-18 | 2014-04-23 | 华中农业大学 | Parturition hastening method for plateau-fish schizothorax oconnori |
| CN104472400A (en) * | 2014-11-11 | 2015-04-01 | 重庆市大足区炫天养殖有限责任公司 | Artificial breeding technology for schizothorax grahami |
| CN105409845A (en) * | 2015-12-01 | 2016-03-23 | 中国科学院昆明动物研究所 | Artificial reproduction method for schizothorax wangchiachii |
| CN106069927A (en) * | 2016-06-24 | 2016-11-09 | 西藏自治区农牧科学院 | A kind of domesticating method of wild Lhasa schizothoracin |
| CN107593536A (en) * | 2017-11-13 | 2018-01-19 | 张荣富 | A kind of schizothoracin breeds cultural method |
| CN108283152A (en) * | 2018-03-28 | 2018-07-17 | 武汉中科瑞华生态科技股份有限公司 | A kind of long filament schizothoracin artificial fecundation method |
| CN208144206U (en) * | 2018-03-28 | 2018-11-27 | 西藏自治区农牧科学院水产科学研究所 | A kind of Lhasa schizothoracin incubating oosperm device |
| CN108324708A (en) * | 2018-03-29 | 2018-07-27 | 中国水产科学研究院长江水产研究所 | A kind of Lhasa schizothoracin anesthesia |
Non-Patent Citations (1)
| Title |
|---|
| 张驰: "野生拉萨裂腹鱼驯化技术初步研究", 《水产养殖》 * |
Cited By (13)
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|---|---|---|---|---|
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| CN110521643A (en) * | 2019-10-08 | 2019-12-03 | 西藏自治区农牧科学院水产科学研究所 | A kind of Lhasa schizothoracin method for incubating oosperm |
| CN111919797A (en) * | 2020-08-20 | 2020-11-13 | 西南大学 | Method for improving survival rate of fertilized eggs of schizothorax laggera |
| CN111919796A (en) * | 2020-08-20 | 2020-11-13 | 西南大学 | Method for adjusting propagation cycle of Lasa schizothorax prenanti |
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