CN110235818B - Method for cultivating loach fry in skeg plateau - Google Patents
Method for cultivating loach fry in skeg plateau Download PDFInfo
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- 239000012286 potassium permanganate Substances 0.000 claims description 5
- CPKVUHPKYQGHMW-UHFFFAOYSA-N 1-ethenylpyrrolidin-2-one;molecular iodine Chemical compound II.C=CN1CCCC1=O CPKVUHPKYQGHMW-UHFFFAOYSA-N 0.000 claims description 4
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- IWVCMVBTMGNXQD-UHFFFAOYSA-N terramycin dehydrate Natural products C1=CC=C2C(O)(C)C3C(O)C4C(N(C)C)C(O)=C(C(N)=O)C(=O)C4(O)C(O)=C3C(=O)C2=C1O IWVCMVBTMGNXQD-UHFFFAOYSA-N 0.000 description 2
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/13—Prevention or treatment of fish diseases
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
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- Biodiversity & Conservation Biology (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a method for cultivating loach fries on an anterior fin plateau, which comprises the following steps: (1) temporary fry rearing, (2) fry opening, (3) live bait feeding stage, (4) tubificidae feeding stage, (5) feeding training stage, (6) fingerling rearing stage, and (7) disease control; according to the invention, through the accurate control of the 7 steps, the artificial cultivation of the loach fry on the forefin plateau is successfully realized for the first time in China, the fry survival rate reaches more than 92%, and the method is simple and easy to popularize.
Description
Technical Field
The invention belongs to the technical field of fish culture, and particularly relates to a method for cultivating loach fries on an anterior fin plateau.
Background
A fish of the genus Tinospora of the subfamily Tinospora of the family Tinospora of the order Cyprina is elongated in body, gradually becomes flat at the rear part, and is almost unchanged in the height of the tail handle. Short head, blunt front end, slightly depressed. The length of the kiss is equal to or slightly longer than the length of the posterior head of the eye. In the lower part of the mouth, there are papillae on the upper lip and shallow wrinkles on the lower lip. The lower jaw is in a shovel shape and is exposed outside the lip. Has 3 pairs of whiskers. The dorsal fin is longer, the outer edge is truncated, the non-branching fin ray is soft, the starting point is forward, the distance from the starting point to the osculating end is 0.8-0.9 times of the distance from the base of the tail fin, the pectoral fin is longer, the backward extension approaches or reaches the starting point of the ventral fin, and the starting point is opposite to the base of the 1 st-2 th branch fin rays of the dorsal fin. The posterior edge of the tail fin is slightly concave, and the anus is close to the starting point of the hip fin. The body is naked without scale, the lateral line is obvious and complete, and the lateral line is from the lower corner of the branchial hole to the middle part of the caudal peduncle.
Because the cascade development of the Jinshajiang midstream hydropower has certain influence on the wild fish resources of the Profin plateau loaches, in order to protect the wild fishery resources of the Profin plateau loaches, the Wuhanzhongke Ruihua ecological science and technology limited company is entrusted by the Datang Guanyin rock hydropower development limited company to jointly carry out related scientific research work on the Profin plateau loaches, the Profin plateau loaches are successfully bred for the first time in the Guanyin rock fish breeding station in 12 months in 2018, and the fry breeding is successfully cultured in 3 months in 2019. Through literature search, the same public reports as the invention are not found.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide the method for cultivating the plateau loach fries, which successfully realizes artificial cultivation of the plateau loach fries for the first time, and has the advantages of high fry survival rate, simple method and easy popularization.
In order to solve the problems, the invention adopts the technical scheme that:
a method for cultivating loach fry in the sketching plateau comprises the following steps:
(1) temporarily culturing the fish fry: temporarily culturing the fry in a large plastic basin with the diameter of 50cm at the water temperature of 15-16 ℃, keeping the water temperature at 15-16 ℃ by matching 2 heating rods with 300 watts in the culture tank, wherein the depth of the water in the basin is 10cm, the plastic basin is floated and placed in a culture tank with the diameter of 3m and the depth of 1m, and the day and night temperature difference caused by too small water in the plastic basin is prevented from being too large, the temporary culture time is 5-6 days, and opening the fry after the yolk sac disappears to one line;
(2) opening the fry: adopting cultured chlorella to perform fry shedding, putting the chlorella into water, dispersing the chlorella into the water, feeding for 2 times every day, wherein the feeding amount of each time is 0.1g/L of water, changing water once after 6 hours after feeding, the water changing amount is 30% of the culture water amount, taking a small amount of fries after the first feeding, observing under a dissecting mirror, if food is found in a digestive tract, indicating that the shedding is successful, then continuously feeding for 5-6 days, and entering a live bait feeding stage when the fries grow more than 6 mm;
(3) and (3) live bait feeding stage: transferring the fry to a 120 x 50 x 40cm culture tank for culture, wherein an oxygen increasing device and a water inlet and outlet pipe are arranged in the culture tank, the water inlet adopts micro-flow water, the water flow is 1-2L/min, the water outlet pipe is overflow water drainage, and the overflow hole is caught by an 80-mesh net to prevent escape; the live bait is hatched fairy shrimp larva, and is fedSoaking fairy shrimp in 10mg/L vitamin C and 10mg/L terramycin solution for 10min, and feeding 3 times per day with a feeding amount of 3g/m3Culturing the water body for 15 days, and entering a tubificidae feeding stage when the length of the fish fry reaches more than 12 mm;
(4) and (3) feeding tubificidae: increasing the water inlet flow of the culture tank to 3-4L/min, adjusting the mesh of overflow hole anti-escape net to 60 meshes, soaking the fed tubificidae in 3% NaCl solution for 30min for sterilization, rinsing with clear water, soaking in 20ppm terramycin for 10min, grinding the tubificidae with mortar, and feeding at a feeding amount of 8-10g/m each time3Feeding 3 times a day in a water body, sucking residual baits by using a siphon after feeding for 4 hours each time to prevent the water quality deterioration caused by the putrefaction of the residual baits, feeding the water earthworms for 8-10 days, and entering a feeding training stage when the body length of the fish fry reaches more than 16 mm;
(5) a food training stage: the method is carried out by adopting a gradient feeding training method, and specifically comprises the steps of gradually increasing the proportion of artificial feed in the tubificidae until all the fry eat the artificial feed, wherein the artificial feed is powdery microcapsule feed, the protein content is more than 40%, after 8-10 days of feeding training, the fry can completely eat the artificial feed, the body length of the fry can reach more than 20mm, and then the fry is transferred to a fry culture stage;
(6) and (3) breeding the fish seeds: adding water into the microcapsule feed, stirring, kneading into long strips, feeding, adding 0.3% of vitamin C and 0.6% of fish oil into the microcapsule feed, feeding for 3 times a day, controlling the daily feeding rate at 3%, absorbing residual bait with a siphon tube every day, controlling the water temperature at 16-18 ℃ and the dissolved oxygen at 8mg/L or more, and feeding for 2 months until the body length of the fry can reach more than 30 mm;
(7) disease control: the main diseases during seedling stage are obliquitosis and bacterial diseases;
the method for preventing and controlling the pipe worms comprises the following steps: fishing out the fry, soaking and disinfecting the fry for 10min by 10mg/L povidone iodine, reducing and detoxifying the fry by 10g/L vitamin C, draining the culture water in the culture tank, disinfecting the culture tank for 20min by 50mg/L potassium permanganate, and then boiling the water to place the fry in the culture tank for normal culture;
methods of treatment of bacterial diseases: after soaking tubificidae in 1g/L of terramycin for 20min, feeding the fry for 1 time every day for 4 days continuously.
Compared with the prior art, the invention has the following obvious beneficial effects:
the method successfully realizes the artificial cultivation of the plateau loach fry in the forefin for the first time in China, comprises temporary fry cultivation, fry opening, live bait feeding stage, tubificidae feeding stage, food training stage, fry cultivation stage and disease control, and realizes the high survival rate cultivation of the plateau loach fry through the accurate control of the 7 steps; in the method, a heating rod is adopted to maintain the constant temperature of the culture water body when the fry is temporarily cultured, the fry is prevented from being stressed and dead caused by overlarge temperature difference between day and night, chlorella with rich protein content is selected when the fry is opened, compared with the traditional yolk liquid, fairy shrimp and microcapsule feed, the chlorella is opened, the chlorella is not easy to die and deteriorate to pollute the water body, the redundant chlorella can also purify the water body to release oxygen, the survival rate of the fry is favorably improved, before live baits are fed, 10mg/L vitamin C and 10mg/L oxytetracycline solution with good effects are selected through experiments to soak fairy shrimps, the fairy shrimp treated by the method can greatly improve the disease resistance of the fry and reduce the death rate of the fry, and in the same stage of feeding the water earthworms, the water earthworms soaked by the oxytetracycline can kill pathogenic bacteria carried by the water earthworms, the feed can also prevent early bacterial diseases of fish fries, 0.3 percent of vitamin C and 0.6 percent of fish oil are creatively added into the microcapsule feed in the fish fry breeding stage, the microcapsule feed fed in the way can obviously improve the resistance of fish bodies and reduce the morbidity of the fish fries, and finally in the pest control stage, 10mg/L of potassium permanganate is adopted for soaking and disinfecting for 10min, and then 10g/L of vitamin C is used for reducing and detoxifying; according to the method, the fry of the loach on the plateau of the forefin is successfully cultivated for the first time through the improvement of various details, and the survival rate of the fry reaches more than 92 percent.
Detailed Description
In order that the objects and advantages of the invention will be more clearly understood, the following description is given in conjunction with the accompanying examples. It is to be understood that the following text is merely illustrative of one or more specific embodiments of the invention and does not strictly limit the scope of the invention as specifically claimed.
Example 1
A method for cultivating loach fry in the sketching plateau comprises the following steps:
(1) temporarily culturing the fish fry: temporarily culturing 500 fries in a large plastic basin with the diameter of 50cm at the water temperature of 16 ℃, wherein the water depth in the basin is 10cm, the plastic basin is floated and placed in a culture tank with the diameter of 3m and the water depth of 1m, 2 heating rods with 300 watts are matched in the culture tank, the water temperature is kept at 16 ℃, and the phenomenon that the temperature difference between day and night is too large due to too small water amount in the plastic basin is prevented, the temporary culture time is 6 days, and opening of the fries is carried out when yolk sacs disappear to one line;
(2) opening the fry: adopting cultured chlorella to perform fry shedding, putting the chlorella into water, dispersing the chlorella into the water, feeding for 2 times every day, wherein the feeding amount of each time is 0.1g/L of water, changing water once after 6 hours after feeding, the water changing amount is 30% of the culture water amount, taking a small amount of fries after the first feeding, observing under a dissecting mirror, if food is found in a digestive tract, indicating that the shedding is successful, then continuously feeding for 6 days, and entering a live bait feeding stage when the fries grow more than 6 mm;
(3) and (3) live bait feeding stage: transferring the fry to a 120 x 50 x 40cm culture tank for culture, wherein an oxygen increasing device and a water inlet and outlet pipe are arranged in the culture tank, the water inlet adopts micro flowing water, the water flow is 1.8L/min, the water outlet pipe is used for overflow drainage, and the overflow holes are caught by a 80-mesh net to prevent escape; the live bait is hatched fairy shrimp larva, and is prepared by soaking fairy shrimp in 10mg/L vitamin C and 10mg/L terramycin solution for 10min before feeding, and feeding 3 times per day with a feeding amount of 3g/m3Culturing the water body for 15 days, and entering a tubificidae feeding stage when the length of the fish fry reaches more than 12 mm;
(4) and (3) feeding tubificidae: the water inlet flow of the culture tank is increased to 3.6L/min and overflowsAdjusting the mesh of the flow hole anti-escape net to be 60 meshes, soaking the fed tubificidae in 3% NaCl solution for 30min for disinfection, then rinsing the tubificidae clean with clear water, soaking the tubificidae in 20ppm terramycin for 10min, crushing the tubificidae with a mortar, and feeding the tubificidae, wherein the feeding amount is 9g/m each time3Feeding 3 times a day in a water body, sucking residual baits by using a siphon after feeding for 4 hours each time to prevent the water quality deterioration caused by the putrefaction of the residual baits, feeding the water earthworms for 9 days, and entering a feeding training stage when the body length of the fish fry reaches more than 16 mm;
(5) a food training stage: the method is carried out by adopting a gradient feeding training method, and specifically comprises the steps of gradually increasing the proportion of artificial feed in tubificidae until the fry completely ingests the artificial feed, wherein the artificial feed is powdery microcapsule feed (purchased from research center of feeds for Shandong Shengsuo fishing, crude protein is more than 50%, crude fat is more than 8%, crude fiber is less than 3%, crude ash is less than 16.5%, calcium is less than 5%, total phosphorus is more than 1%, water is less than 12%, lysine is more than 2%), and the protein content is more than 40%, after 10 days of feeding training, the fry can completely ingest the artificial feed, the body length of the fry can reach more than 20mm, and then the fry is transferred to a fry culture stage;
(6) and (3) breeding the fish seeds: adding water into the microcapsule feed, stirring and kneading the microcapsule feed into long strips for feeding, adding 0.3 wt% of vitamin C and 0.6 wt% of fish oil into the microcapsule feed, feeding for 3 times a day, controlling the daily feeding rate to be 3%, absorbing residual bait by a siphon tube every day, controlling the water temperature to be 17 ℃, controlling the dissolved oxygen to be more than 8mg/L, and feeding for 2 months until the body length of the fry can reach more than 30 mm;
(7) disease control: the main diseases during seedling stage are obliquitosis and bacterial diseases;
the method for preventing and controlling the pipe worms comprises the following steps: fishing out the fry, soaking and disinfecting the fry for 10min by 10mg/L povidone iodine, reducing and detoxifying the fry by 10g/L vitamin C, draining the culture water in the culture tank, disinfecting the culture tank for 20min by 50mg/L potassium permanganate, and then boiling the water to place the fry in the culture tank for normal culture;
methods of treatment of bacterial diseases: after soaking tubificidae in 1g/L of terramycin for 20min, feeding the fry for 1 time every day for 4 days continuously.
After the cultivation of the steps, 468 surviving seedlings are finally obtained, and the survival rate reaches 93.6%.
Example 2
A method for cultivating loach fry in the sketching plateau comprises the following steps:
(1) temporarily culturing the fish fry: temporarily culturing 500 fries in a large plastic basin with the diameter of 50cm at the water temperature of 15 ℃, wherein the water depth in the basin is 10cm, the plastic basin is floated and placed in a culture tank with the diameter of 3m and the water depth of 1m, 2 heating rods with 300 watts are matched in the culture tank, the water temperature is kept at 15 ℃, and the phenomenon that the temperature difference between day and night is too large due to too small water amount in the plastic basin is prevented, the temporary culture time is 5 days, and opening of the fries is carried out when yolk sacs disappear to one line;
(2) opening the fry: adopting cultured chlorella to perform fry shedding, putting the chlorella into water, dispersing the chlorella into the water, feeding for 2 times every day, wherein the feeding amount of each time is 0.1g/L of water, changing water once after 6 hours after feeding, the water changing amount is 30% of the culture water amount, taking a small amount of fries after the first feeding, observing under a dissecting mirror, if food is found in a digestive tract, indicating that the shedding is successful, then continuously feeding for 6 days, and entering a live bait feeding stage when the fries grow more than 6 mm;
(3) and (3) live bait feeding stage: transferring the fry to a 120 x 50 x 40cm culture tank for culture, wherein an oxygen increasing device and a water inlet and outlet pipe are arranged in the culture tank, the water inlet adopts micro flowing water, the water flow is 2L/min, the water outlet pipe adopts overflow drainage, and the overflow holes are caught by a 80-mesh net to prevent escape; the live bait is hatched fairy shrimp larva, and is prepared by soaking fairy shrimp in 10mg/L vitamin C and 10mg/L terramycin solution for 10min before feeding, and feeding 3 times per day with a feeding amount of 3g/m3Culturing the water body for 15 days, and entering a tubificidae feeding stage when the length of the fish fry reaches more than 12 mm;
(4) and (3) feeding tubificidae: increasing the water inlet flow of the culture tank to 4L/min, adjusting the mesh of the overflow hole anti-escape net to 60 meshes, soaking the fed tubificidae in 3% NaCl solution for 30min for sterilization, then rinsing with clear water, soaking in 20ppm terramycin for 10min, grinding the tubificidae with a mortar, and feeding, wherein the feeding amount is 10g/m each time3Feeding 3 times a day in water 4h each timeSucking residual bait by a siphon to prevent water quality deterioration caused by the putrefaction of the residual bait, feeding the residual bait by using water earthworms for 10 days, and entering a feeding training stage when the body length of the fry reaches more than 16 mm;
(5) a food training stage: the method is carried out by adopting a gradient feeding training method, and specifically comprises the steps of gradually increasing the proportion of artificial feed in tubificidae until the fry completely ingests the artificial feed, wherein the artificial feed is powdery microcapsule feed (purchased from research center of feeds for Shandong Shengsuo fishing, crude protein is more than 50%, crude fat is more than 8%, crude fiber is less than 3%, crude ash is less than 16.5%, calcium is less than 5%, total phosphorus is more than 1%, water is less than 12%, lysine is more than 2%), and the protein content is more than 40%, after 10 days of feeding training, the fry can completely ingest the artificial feed, the body length of the fry can reach more than 20mm, and then the fry is transferred to a fry culture stage;
(6) and (3) breeding the fish seeds: adding water into the microcapsule feed, stirring and kneading the microcapsule feed into long strips for feeding, adding 0.3% of vitamin C and 0.6% of fish oil into the microcapsule feed, feeding for 3 times a day, controlling the daily feeding rate to be 3%, absorbing residual bait by a siphon tube every day, controlling the water temperature to be 18 ℃ and the dissolved oxygen to be more than 8mg/L, and feeding for 2 months to ensure that the body length of the fry can reach more than 30 mm;
(7) disease control: the main diseases during seedling stage are obliquitosis and bacterial diseases;
the method for preventing and controlling the pipe worms comprises the following steps: fishing out the fry, soaking and disinfecting the fry for 10min by 10mg/L povidone iodine, reducing and detoxifying the fry by 10g/L vitamin C, draining the culture water in the culture tank, disinfecting the culture tank for 20min by 50mg/L potassium permanganate, and then boiling the water to place the fry in the culture tank for normal culture;
methods of treatment of bacterial diseases: after soaking tubificidae in 1g/L of terramycin for 20min, feeding the fry for 1 time every day for 4 days continuously.
After the cultivation of the steps, 461-tailed surviving offspring seeds are finally obtained, and the survival rate reaches 92.2 percent.
Finally, it is to be noted that: the above embodiments do not limit the invention in any way, and it is obvious to those skilled in the art that modifications and improvements can be made on the basis of the present invention. Accordingly, any modification made without departing from the spirit of the invention is within the scope of the claims.
Claims (1)
1. A method for cultivating the fry of the loach on the skeg plateau is characterized by comprising the following steps:
(1) temporarily culturing the fish fry: temporarily culturing the fry in a large plastic basin with the diameter of 50cm at the water temperature of 15-16 ℃, keeping the water temperature at 15-16 ℃ by matching 2 heating rods with 300 watts in the culture tank, wherein the depth of the water in the basin is 10cm, the plastic basin is floated and placed in a culture tank with the diameter of 3m and the depth of 1m, and the day and night temperature difference caused by too small water in the plastic basin is prevented from being too large, the temporary culture time is 5-6 days, and opening the fry after the yolk sac disappears to one line;
(2) opening the fry: adopting cultured chlorella to perform fry shedding, putting the chlorella into water, dispersing the chlorella into the water, feeding for 2 times every day, wherein the feeding amount of each time is 0.1g/L of water, changing water once after 6 hours after feeding, the water changing amount is 30% of the culture water amount, taking a small amount of fries after the first feeding, observing under a dissecting mirror, if food is found in a digestive tract, indicating that the shedding is successful, then continuously feeding for 5-6 days, and entering a live bait feeding stage when the fries grow more than 6 mm;
(3) and (3) live bait feeding stage: transferring the fry to a 120 x 50 x 40cm culture tank for culture, wherein an oxygen increasing device and a water inlet and outlet pipe are arranged in the culture tank, the water inlet adopts micro-flow water, the water flow is 1-2L/min, the water outlet pipe is overflow water drainage, and the overflow hole is caught by an 80-mesh net to prevent escape; the live bait is hatched fairy shrimp larva, and is prepared by soaking fairy shrimp in 10mg/L vitamin C and 10mg/L terramycin solution for 10min before feeding, and feeding 3 times per day with a feeding amount of 3g/m3Culturing the water body for 15 days, and entering a tubificidae feeding stage when the length of the fish fry reaches more than 12 mm;
(4) and (3) feeding tubificidae: increasing the water inlet flow of the culture tank to 3-4L/min, adjusting the mesh of overflow hole anti-escape net to 60 meshes, soaking the fed tubificidae in 3% NaCl solution for 30min for sterilization, rinsing with clear water, soaking in 20ppm terramycin for 10min, grinding the tubificidae with mortar, and feeding at a feeding amount of 8-1 per time0g/m3Feeding 3 times a day in a water body, sucking residual baits by using a siphon after feeding for 4 hours each time to prevent the water quality deterioration caused by the putrefaction of the residual baits, feeding the water earthworms for 8-10 days, and entering a feeding training stage when the body length of the fish fry reaches more than 16 mm;
(5) a food training stage: the method is carried out by adopting a gradient feeding training method, and specifically comprises the steps of gradually increasing the proportion of artificial feed in the tubificidae until all the fry eat the artificial feed, wherein the artificial feed is powdery microcapsule feed, the protein content is more than 40%, after 8-10 days of feeding training, the fry can completely eat the artificial feed, the body length of the fry can reach more than 20mm, and then the fry is transferred to a fry culture stage;
(6) and (3) breeding the fish seeds: adding water into the microcapsule feed, stirring, kneading into long strips, feeding, adding 0.3% of vitamin C and 0.6% of fish oil into the microcapsule feed, feeding for 3 times a day, controlling the daily feeding rate at 3%, absorbing residual bait with a siphon tube every day, controlling the water temperature at 16-18 ℃ and the dissolved oxygen at 8mg/L or more, and feeding for 2 months until the body length of the fry can reach more than 30 mm;
(7) disease control: the main diseases during seedling stage are obliquitosis and bacterial diseases;
the method for preventing and controlling the pipe worms comprises the following steps: fishing out the fry, soaking and disinfecting the fry for 10min by 10mg/L povidone iodine, reducing and detoxifying the fry by 10g/L vitamin C, draining the culture water in the culture tank, disinfecting the culture tank for 20min by 50mg/L potassium permanganate, and then boiling the water to place the fry in the culture tank for normal culture;
methods of treatment of bacterial diseases: after soaking tubificidae in 1g/L of terramycin for 20min, feeding the fry for 1 time every day for 4 days continuously.
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