CN102007873A - Intensive artificial propagation method for Myxocyprinus asiaticus - Google Patents

Intensive artificial propagation method for Myxocyprinus asiaticus Download PDF

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CN102007873A
CN102007873A CN200910044281XA CN200910044281A CN102007873A CN 102007873 A CN102007873 A CN 102007873A CN 200910044281X A CN200910044281X A CN 200910044281XA CN 200910044281 A CN200910044281 A CN 200910044281A CN 102007873 A CN102007873 A CN 102007873A
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parent population
female parent
brown sheet
spawning pond
laying nest
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王国平
廖伏初
曾南华
高四新
周曙光
陈满
王浩
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杨述奇
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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Abstract

An intensive artificial propagation method for Myxocyprinus asiaticus comprises the steps of parent fish selection, induced spawning and hatching. Wherein the induced spawning comprises preparing oxytocic in different doses, administering the oxytocic timely, and designing an oviposition-nest and an oviposition pool, and the hatching comprises designing a hatching pool and determining the special hatching condition. The method of the present invention allows the fry of Myxocyprinus asiaticus to be bred in a large number, the original germplasm to be ensured, the incidence of disease to be reduced, and the survival rate of Myxocyprinus asiaticus to be improved.

Description

Mullet large-scale artificial propagation method
Technical field
The present invention relates to the freshwater fish culturing method, further be meant mullet large-scale artificial propagation method.
Background technology
Mullet belongs to Cypriniformes, Catostomidae, Myxocyprinus.Be commonly called as and burn bream, yellow row, wooden leaf dish, snapper, blood row, powder row etc., mainly be distributed in ground such as the Changjiang river, Jinsha jiang River.Dongting Lake fattening is gone in feeding migration, lives in lake, the river.The young and adult come in every shape, habitat and biological habit are not quite similar, the juvenile fish happiness collects dwells between the gravel that current delay, multi-activity is in the water body upper strata, the adult happiness is at the spacious pool of rivers, omnivorousness, staple food invertebrate and insect larvae, also organic substance at the bottom of the drinking water is sucked the diatom and the phytoclasts that adhere to.Because the change of ecotope and cruel fish are excessively captured, its resource slump of disastrous proportions now is national second class protection animal.The mullet lovely luster, fine figure, nutritious, both had a sight, be again high-grade dining table delicacies.Mullet adapts to various breed waters, particularly large tracts of land, the foster best kind of pond cover, has good breed, DEVELOPMENT PROSPECT.Because mullet germ plasm resource is degenerated, and the seed breeding rate is low, caused very big influence at present to artificial ecology breed and artificial releasing's scale.
Summary of the invention
The technical problem to be solved in the present invention is; defective at the prior art existence; propose a kind of mullet large-scale artificial propagation method, can breed the kermes fry in a large number and guarantee the original seedization of its germplasm, can also reduce disease, improve the survival rate of mullet by this method.
Technical scheme of the present invention is that described mullet large-scale artificial propagation method is:
(1) parent population is selected: select that scale is complete, the proportioning of female parent population and male parent population (quantity compares) is not 1: 1 to body weight in the mature brood fish of 8kg at the end;
Mature brood fish is: male parent population gently presses belly to have the milky seminal fluid to flow out, and entry is promptly loose; Female parent population belly expands, and the ovary profile can be seen up by fish belly portion, and the ovum grain size that egg apparatus digs out is neatly full with digging, and disperses, and diameter surpasses 2mm;
(2) hasten parturition:
A. oxytocic drug: the mixture of human chorionic gonadtropin (HCG), luteotropin releasing hormone d-ala analog (LRH-A), maleic acid DOM (DOM), carp, four kinds of medicines of crucian carp brain hypophysis (PG);
B. the total consumption of oxytocic drug of the female parent population body weight of per kilogram:
Figure B200910044281XD0000021
The oxytocic drug consumption of the male parent population body weight of per kilogram is half of above-mentioned consumption;
C. injection system: adopt three injections, injection for the first time and only inject female parent population for the second time; Female parent population carried out first time injection in preceding 30 days hastening parturition, drug dose is 1/10th of the total consumption of described medicine; When hastening parturition female parent population is carried out the injection second time, drug dose is 2/10ths of the total consumption of described medicine; Female parent population injected for the third time in back 24 hours apart from injecting for the second time, the surplus medicine has all been annotated; When injecting for the third time, male parent population is injected by described drug dose (half of the total consumption of female parent population oxytocic drug); Per kilogram parent population injection drug liquid measure is 2.5ml;
D. will finish the parent population of above-mentioned oxytocic drug injection and put into spawning pond, keeping the flow velocity of water in the spawning pond is 0.08 meter per second-0.12 meter per second, to stimulate the parent population gonad development; Place laying nest in the spawning pond;
A kind of embodiment preparation method of laying nest is: tack brown sheet with cotton rope (as the PEF line), more brown sheet is hung on the bamboo bar, adjacent two cotton ropes are spaced apart 25cm-35cm on the bamboo bar, and brown sheet (laying nest) quantity is 10 times of female parent population number;
E. parent population after laying eggs and finishing catches out parent population, takes out laying nest (attached ovum palm fibre sheet), laying nest (attached ovum palm fibre sheet) is put into the hatchery carry out the flowing water hatching;
(3) hatching: after will cleaning with clear water by the laying nest that takes out in the described spawning pond (attached ovum palm fibre sheet), used 3ppm potassium permanganate solution soaking again 8 seconds-12 seconds, use cotton rope (as the PEF line) to be hung on the steady arm in the hatchery then and laying nest is suspended in the water, and the spacing that makes adjacent two laying nests on the steady arm is 28cm-32cm, and the hatchery water velocity remains on 0.08 meter per second-0.12 meter per second;
Flow into downstream after fry hatches and connect the seedling case, after fry reaches some in the case, promptly drag for into the conventional incubation groove that water plant is arranged and continue hatching, when the disappearance of fry yolk sac is in flat trip, can emerge.
Mullet large-scale artificial propagation method of the present invention; it is the integrated practical experience of enriching of being engaged in aquatic products work decades of applicant; on basis by deep theoretical research and analysis; just establish through a large amount of and test repeatedly over a long time; be the important achievement that fish genetic breeding aspect obtains, test site successful reproduction fry in enormous quantities.The inventive method improves parent population spawning rate 50% than commonsense method, improve incubation rate 45%, improve seed and culture survival rate 50%, improve adult fish culture survival rate 48%, spawning rate, incubation rate, seed are cultured survival rate, the adult fish culture survival rate all maintains the leading position in the same industry.
As known from the above, the present invention is a mullet large-scale artificial propagation method, and this method can be bred the kermes fry in a large number and be guaranteed the original seedization of its germplasm, can also reduce disease, improve the survival rate of mullet.
Embodiment
Embodiment 1: mullet large-scale artificial propagation method is:
(1) parent population is selected: select that scale is complete, the proportioning of female parent population and male parent population (quantity compares) is not 1: 1 to body weight in the mature brood fish of 8kg at the end;
Mature brood fish is: male parent population gently presses belly to have the milky seminal fluid to flow out, and entry is promptly loose; Female parent population belly expands, and the ovary profile can be seen up by fish belly portion, and the ovum grain size that egg apparatus digs out is neatly full with digging, and disperses, and diameter surpasses 2mm;
(2) hasten parturition:
A. oxytocic drug: the mixture of human chorionic gonadtropin (HCG), luteotropin releasing hormone d-ala analog (LRH-A), maleic acid DOM (DOM), carp, four kinds of medicines of crucian carp brain hypophysis (PG);
B. the total consumption of oxytocic drug of the female parent population body weight of per kilogram:
Figure B200910044281XD0000041
The oxytocic drug consumption of the male parent population body weight of per kilogram is half of above-mentioned consumption;
C. injection system: adopt three injections, injection for the first time and only inject female parent population for the second time; Female parent population carried out first time injection in preceding 30 days hastening parturition, drug dose is 1/10th of the total consumption of described medicine; When hastening parturition female parent population is carried out the injection second time, drug dose is 2/10ths of the total consumption of described medicine; Female parent population injected for the third time in back 24 hours apart from injecting for the second time, the surplus medicine has all been annotated; When injecting for the third time, male parent population is injected by described drug dose (half of the total consumption of female parent population oxytocic drug); Per kilogram parent population injection drug liquid measure is 2.5ml;
D. will finish the parent population of above-mentioned oxytocic drug injection and put into spawning pond, keeping the flow velocity of water in the spawning pond is 0.1 meter per second, to stimulate the parent population gonad development; Place laying nest in the spawning pond;
A kind of embodiment preparation method of laying nest is: tack brown sheet with cotton rope (as the PEF line), more brown sheet is hung on the bamboo bar, adjacent two cotton ropes are spaced apart 30cm on the bamboo bar, and brown sheet (laying nest) quantity is 10 times of female parent population number;
The length of described bamboo bar decide on spawning pond size, and brown sheet size generally is the size of the brown sheet of nature, during use the hard part of brown sheet is removed to get final product;
For preventing the loss of ovum grain, also can place brown sheet, in order to connect ovum in the spawning pond bottom;
E. parent population after laying eggs and finishing catches out parent population, takes out laying nest (attached ovum palm fibre sheet), laying nest (attached ovum palm fibre sheet) is put into the hatchery carry out the flowing water hatching;
(3) hatching: after will cleaning with clear water by the laying nest that takes out in the described spawning pond (attached ovum palm fibre sheet), use 3ppm potassium permanganate solution soaking 10 seconds again, use cotton rope (as the PEF line) to be hung on the steady arm in the hatchery then and laying nest is suspended in the water, and the spacing that makes adjacent two laying nests on the steady arm is 28cm-32cm, and the hatchery water velocity remains on 0.1 meter per second;
Flow into downstream after fry hatches and connect the seedling case, after fry reaches some in the case, promptly drag for into the conventional incubation groove that water plant is arranged and continue hatching, when the disappearance of fry yolk sac is in flat trip, can emerge.
Generally, when general water temperature was 13 ℃-15 ℃, the incubation of membrane time was 9 days-11 days; Water temperature is in the time of 18 ℃-20 ℃, and the incubation of membrane time is 5 days-7 days.What pay special attention to is to rise in the previous day of membrane to stop to use any medicine, avoids poisoning.
Steady arm in the described hatchery can be made annular with the galvanized pipe of 15cm-20cm caliber, in spawning pond, is used to hang laying nest with three stent support.
Embodiment 2: in the mullet large-scale artificial propagation method, and the total consumption of oxytocic drug of the female parent population body weight of per kilogram:
Figure B200910044281XD0000051
The oxytocic drug consumption of the male parent population body weight of per kilogram is half of above-mentioned consumption;
All the other processes are identical with embodiment 1.
Embodiment 3: in the mullet large-scale artificial propagation method, and the total consumption of oxytocic drug of the female parent population body weight of per kilogram:
The oxytocic drug consumption of the male parent population body weight of per kilogram is half of above-mentioned consumption; All the other processes are identical with embodiment 1.

Claims (4)

1. mullet large-scale artificial propagation method is characterized in that this method is:
(1) parent population is selected: select that scale is complete, the proportioning of female parent population and male parent population (quantity compares) is not 1: 1 to body weight in the mature brood fish of 8kg at the end;
Mature brood fish is: male parent population gently presses belly to have the milky seminal fluid to flow out, and entry is promptly loose; Female parent population belly expands, and the ovary profile can be seen up by fish belly portion, and the ovum grain size that egg apparatus digs out is neatly full with digging, and disperses, and diameter surpasses 2mm;
(2) hasten parturition:
A. oxytocic drug: the mixture of human chorionic gonadtropin HCG, luteotropin releasing hormone d-ala analog LRH-A, maleic acid DOM DOM, carp, four kinds of medicines of crucian carp brain hypophysis PG;
B. the total consumption of oxytocic drug of the female parent population body weight of per kilogram:
Figure F200910044281XC0000011
The oxytocic drug consumption of the male parent population body weight of per kilogram is half of above-mentioned consumption;
C. injection system: adopt three injections, injection for the first time and only inject female parent population for the second time; Female parent population carried out first time injection in preceding 30 days hastening parturition, drug dose is 1/10th of the total consumption of described medicine; When hastening parturition female parent population is carried out the injection second time, drug dose is 2/10ths of the total consumption of described medicine; Female parent population injected for the third time in back 24 hours apart from injecting for the second time, the surplus medicine has all been annotated; When injecting for the third time, male parent population is injected by described drug dose (half of the total consumption of female parent population oxytocic drug); Per kilogram parent population injection drug liquid measure is 2.5ml;
D. will finish the parent population of above-mentioned oxytocic drug injection and put into spawning pond, keeping the flow velocity of water in the spawning pond is 0.08 meter per second-0.12 meter per second, to stimulate the parent population gonad development; Place laying nest in the spawning pond;
A kind of embodiment preparation method of laying nest is: tack brown sheet with cotton rope, more brown sheet is hung on the bamboo bar, adjacent two PEF lines are spaced apart 25cm-35cm on the bamboo bar, and brown sheet quantity is 10 times of female parent population number;
The length of described bamboo bar decide on spawning pond size, and brown sheet size generally is the size of the brown sheet of nature, during use the hard part of brown sheet is removed to get final product;
For preventing the loss of ovum grain, also can place brown sheet, in order to connect ovum in the spawning pond bottom;
E. parent population after laying eggs and finishing catches out parent population, takes out laying nest, laying nest is put into the hatchery carry out the flowing water hatching;
(3) hatching: after will cleaning with clear water by the laying nest that takes out in the described spawning pond, used 3ppm potassium permanganate solution soaking again 8 seconds-12 seconds, be hung on the steady arm in the hatchery with cotton rope then and laying nest is suspended in the water, and the spacing that makes adjacent two laying nests on the steady arm is 28cm-32cm, and the hatchery water velocity remains on 0.08 meter per second-0.12 meter per second;
Flow into downstream after fry hatches and connect the seedling case, after fry reaches some in the case, promptly drag for into the conventional incubation groove that water plant is arranged and continue hatching, when the disappearance of fry yolk sac is in flat trip, can emerge.
2. according to the described mullet large-scale artificial of claim 1 propagation method, it is characterized in that the steady arm in the described hatchery is made annular with the galvanized pipe of 15cm-20cm caliber, in spawning pond, be used to hang laying nest with three stent support.
3. according to the described mullet large-scale artificial of claim 1 propagation method; it is characterized in that; a kind of embodiment preparation method of described laying nest is: tack brown sheet with cotton rope; again brown sheet is hung on the bamboo bar; adjacent two cotton ropes are spaced apart 30cm on the bamboo bar, brown sheet quantity is 10 times of female parent population number.
4. according to the described mullet large-scale artificial of claim 1 propagation method, it is characterized in that,, place brown sheet in the spawning pond bottom, in order to connect ovum for preventing the loss of ovum grain.
CN200910044281XA 2009-09-07 2009-09-07 Intensive artificial propagation method for Myxocyprinus asiaticus Pending CN102007873A (en)

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102218133A (en) * 2011-05-26 2011-10-19 浙江省淡水水产研究所 Injection oxytocin for loach
CN102228681A (en) * 2011-06-09 2011-11-02 吴江市水产养殖有限公司 Fish spawning inducer and spawning induction method
CN103392658A (en) * 2013-08-22 2013-11-20 苏州市相城区新时代特种水产养殖场 Mullet culture method
CN103461252A (en) * 2013-09-29 2013-12-25 四川省农业科学院水产研究所 Method for rearing and maturing myxocyprinus asiaticus parent fish
CN104170778A (en) * 2014-08-18 2014-12-03 永丰一帆水产科技有限公司 Large-scale common sucker breeding method capable of controlling temperature of circulating water
CN104621016A (en) * 2014-11-17 2015-05-20 苏州市相城区盛胡特种养殖专业合作社 Breeding method for buffalo perches
CN105994066A (en) * 2016-06-06 2016-10-12 苏州福德龙水产养殖有限公司 Myxocyprinus asiaticus yield-increase breeding method

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102218133A (en) * 2011-05-26 2011-10-19 浙江省淡水水产研究所 Injection oxytocin for loach
CN102228681A (en) * 2011-06-09 2011-11-02 吴江市水产养殖有限公司 Fish spawning inducer and spawning induction method
CN103392658A (en) * 2013-08-22 2013-11-20 苏州市相城区新时代特种水产养殖场 Mullet culture method
CN103461252A (en) * 2013-09-29 2013-12-25 四川省农业科学院水产研究所 Method for rearing and maturing myxocyprinus asiaticus parent fish
CN104170778A (en) * 2014-08-18 2014-12-03 永丰一帆水产科技有限公司 Large-scale common sucker breeding method capable of controlling temperature of circulating water
CN104621016A (en) * 2014-11-17 2015-05-20 苏州市相城区盛胡特种养殖专业合作社 Breeding method for buffalo perches
CN105994066A (en) * 2016-06-06 2016-10-12 苏州福德龙水产养殖有限公司 Myxocyprinus asiaticus yield-increase breeding method

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Application publication date: 20110413