CN104488760B - Artificial propagation method for mystus macropterus - Google Patents
Artificial propagation method for mystus macropterus Download PDFInfo
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- CN104488760B CN104488760B CN201410675074.5A CN201410675074A CN104488760B CN 104488760 B CN104488760 B CN 104488760B CN 201410675074 A CN201410675074 A CN 201410675074A CN 104488760 B CN104488760 B CN 104488760B
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- digestive
- tract
- water
- big fin
- artificial
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention provides an artificial propagation method for mystus macropterus. The artificial propagation method comprises the following steps of fry feeding, artificial oxytocin injection, spawn taking and sperm collection, fertilization, incubation and temporary rearing and cultivating. LHRH (luteinizing hormone releasing hormone)-A2, DOM (dissolved organic matters) and HCG (human chorionic gonadotropin) serve as the oxytocin, the spawning rate in artificial hastening parturition reaches 74%, a circumstance that propagation is carried out by killing the mystus macropterus is avoided, the spontaneous delivery rate reaches 62%-76%, and the fertility rate reaches 14%-32%. In an incubation process, fertilized spawns are placed in hyperoxic flowing water, the incubation rate reaches 27.5%-76.19%, and the temporary rearing survival rate reaches 87.6%. The fries of the mystus macropterus eat feedstuff with 40% of protein level when the fries are small, the feedstuff is processed by fresh minced fillet and powder, the fries are easy and convenient to feed, and the mystus macropterus can be articially propagated in batches.
Description
Technical field
The invention belongs to fish reproduction technology, especially a kind of without the need for by killing the artificial propagation side that fish takes the big fin Of-digestive-tract of essence
Method.
Background technology
Big fin Of-digestive-tract category Silurformes, is distributed in the Changjiang river to each water system of the Zhujiang River, is bottom dwelling property Fish, and to inhabit current more anxious more, bottom
The bright rivers of many stones of matter are dry, in tributary, like cluster.Prowl at night, with zoobenthos as staple food, such as spiral shell, freshwater mussel, aquatic insect and its
Larva, shrimp, small fish etc., also eat higher plant chip and algae once in a while.The 6-7 months lay eggs on flowing water beach, and ovum is sticked on rock
Developed.The Changjiang river is distributed in each water system of the Zhujiang River, produces more with rivers middle and upper reaches, be one of common edible fish.Big fin
Of-digestive-tract general individuals weigh 0.5 kilogram or so, and maximum individual reachable 5 kilograms, rich in protein, fine and tender taste, taste are also delicious, deep
Liked by consumer, wild big fin Of-digestive-tract can not meet the demand in market at present.
The content of the invention
It is an object of the invention to provide a kind of artificial fecundation method of big fin Of-digestive-tract, need not kill fish can artificial propagation, realize
Breeding scale.
In order to achieve the above object, the present invention provides following technical scheme：
A kind of artificial fecundation method of big fin Of-digestive-tract, comprises the following steps,
1) big fin Of-digestive-tract is fed, if wild big fin Of-digestive-tract, needs to carry out nursing domestication, wild fry with 1.5～
The saline soak of 2% (weight) entered pond after 3～5 minutes, fed Limnodrilus hoffmeisteri within 5～15 days before entering pond, which was fed after 15 days with newly
Raw fish meat paste and the soft particulate material that protein content made by powder is 40% (weight)；If artificial culture fry out, directly
Connect the soft particulate material that 40% (weight) protein content being processed into together with powder with fresh minced fish is fed to which；Day feeding volume is
The 0.1%～4% of body weight, when water temperature is less than 18 degree, reduces feeding volume, and less than 12 degree do not feed, and timing is washed by water daily, opens
Aerator oxygen supplement is opened, water is monthly regularly changed, until which reaches sexual maturity, the ovary of raun reaches fourth stage, milter gonad development
III phase；
2) in spawning pond, 25～33 degree of water temperature is controlled, to big fin Of-digestive-tract manual injection's oxytocic hormones, is spaced 12～15 hours
Afterwards, oxytocic hormone is injected again；
3) raun is carried out squeezing ovum in 20 hours after injecting at second, spermary operation is manually taken to milter；
4) by the spermary collected mortar grinder pulp, the ovum of extrusion is subsequently adding, add water activation, and ceaselessly
Agitation water a few minutes, germ cell is layered in hatchery and is hatched；
5) in hatching process, hatchery control intensity of illumination is covered with double-deck sun net, and add gas exhausting stone aeration to increase
Oxygen is hatched, while water temperature is controlled at 24～27 degree, changes water daily once, changes water twice, and change every time within one day during germ cell membrane
Water half, it is ensured that the oxygen content in pond；
6) temporarily supported after fry membrane, changed water daily once, and water oxygen supplement is stirred with gas exhausting stone inflation, until fry vigor
Strengthen.
Preferably, day feeding volume for body weight 2%.
Further, when carrying out feeding domestication process to wild big fin Of-digestive-tract, using chlorine dioxide to the big fin Of-digestive-tract that has wound
Preventing and treating.
Further, the fry that artificial propagation goes out is put in the culture pond for having cultivated zooplankton, when fry grows to 4cm
During～8cm, which is fed with fresh minced fish and the soft particulate material that protein content made by powder is 40% (weight), day feeding volume
For the 0.1%～4% of body weight.
Further, during nursing domestication being carried out to wild big fin Of-digestive-tract, irregularly adopt strong chlorine oil and DBDMH
Chlorinated product, protozoon are net, metrifonate and avilamycin carry out parasite killing preventing and treating, dosage the using according to the medicament for being used of employing
Bright book is using.
Further, injection oxytocic hormone parent in, raun weight be 220～270 grams, milter weight be 400～
500 grams, the ratio of female milter quantity is 3～5:1.
Further, mixture of the oxytocic hormone for LHRH-A2, DOM, HCG composition, the dosage point of per kilogram of fish injection
Wei not 20～40ug, 20～40mg, 3000～6000IU.
Preferably, the oxytocic hormone dosage of raun is LHRH-A2, DOM, HCG according to every kilogram of 40ug, 40mg, 6000IU
Proportions, the oxytocic hormone dosage of milter is the ratio of LHRH-A2, DOM, HCG according to every kilogram of 20ug, 20mg, 3000IU
Example proportioning.
Further, in step 2) in, spawning pond is flowing water spawning pond, and carries out soaking lixiviating three times, and makes pool wall long
, there is scratch to reduce big fin Of-digestive-tract in spawning pond in periphyton.
Further, in step 4) in, the hatching frame that the screen cloth of setting batten and 30 mesh is made in hatchery, germ cell
It is layered on hatching frame.
Further, in step 5) in the hatching process of germ cell, dead ovum to be absorbed, after fertilization starts on the 4th day,
Dead ovum and dead seedling are absorbed daily, and change water once.
Beneficial effects of the present invention are：
1) using LHRH-A2, DOM, HCG as oxytocic hormone, in making artificial induced spawning, spawning rate reaches 74%；
2) to raun using ovum is squeezed, spermary operation is manually taken to milter, is bred without the need for by killing big fin Of-digestive-tract, natural labor
Rate 62～76%, rate of fertilization 14%～32%；
3) in hatching process, germ cell is placed in the circulating water of high dissolved oxygen, incubation rate reaches 27.5～76.19%,
Motility rate 87.6% is temporarily formed, breeding scale is realized；
4) fry of big fin Of-digestive-tract contains in 40% (weight) albumen by made by fresh minced fish is with powder of more just ingesting
The feedstuff of amount, feeds simple and convenient.
Description of the drawings
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
Accompanying drawing to be used needed for having technology description is briefly described, it should be apparent that, drawings in the following description are only this
Some embodiments of invention, for those of ordinary skill in the art, on the premise of not paying creative work, can be with
Other accompanying drawings are obtained according to these accompanying drawings.
Fig. 1 is the flow chart of the present invention；
Specific embodiment
Below in conjunction with the accompanying drawing of the present invention, technical scheme is clearly and completely described, it is clear that institute
The embodiment of description is only a part of embodiment of the invention, rather than the embodiment of whole.Based on the embodiment in the present invention,
The every other embodiment obtained under the premise of creative work is not made by those of ordinary skill in the art, belongs to this
The scope of bright protection.
Embodiment 1
It is with reference to shown in Fig. 1, following for one embodiment of the present of invention：
1) big fin Of-digestive-tract fries are fed, until which reaches sexual maturity
If wild big fin Of-digestive-tract, in addition it is also necessary to carry out nursing domestication.In this process, fry with 1.5～2% salt water logging
Bubble entered pond after 3～5 minutes, fed Limnodrilus hoffmeisteri within 5-15 days before entering pond.Its fry is fed after 15 days with fresh minced fish and powder one
Act the soft particulate material of 40% (weight) protein content being processed into, day feeding volume for body weight 2%.When water temperature is less than 18 degree,
Feeding volume is gradually decreased, less than 12 degree do not feed.Fishpond timing to feeding daily is washed by water, and every month, water was changed in timing, it is ensured that water
Matter is good.Meanwhile, daily time opening aerator, it is ensured that parent fish not anoxia.So feed, until the ovary of raun reaches the IVth
Phase, III phase of milter gonad development.
If artificial culture fry out, 40% be processed into together with powder with fresh minced fish is directly fed to which
The soft particulate material of (weight) protein content.
2) to big fin Of-digestive-tract manual injection's oxytocic hormones
Parent is selected, raun weight is 220～270 grams, and milter weight is 400～500 grams, and the ratio of female milter quantity exists
3～5:It is optimal between 1.In the present embodiment, raun is 22, and milter is 6.27 degree of water temperature of control, urges to big fin Of-digestive-tract manual injections
Produce hormone.
The oxytocic hormone dosage of raun is matched somebody with somebody according to the ratio of every kilogram of 40ug, 40mg, 6000IU for LHRH-A2, DOM, HCG
Than the oxytocic hormone dosage of milter is the proportions of LHRH-A2, DOM, HCG according to every kilogram of 20ug, 20mg, 3000IU.Between
After 12 hours, the oxytocic hormone of identical type and same dose is injected again.
3) squeeze ovum and take essence
Spawning pond is flowing water spawning pond, and carries out soaking lixiviating three times, and allows the long periphyton of pool wall, to reduce big fin Of-digestive-tract
There is scratch in spawning pond.In 20 hours after second injection oxytocic hormone, raun is carried out squeezing ovum, while to hero
Fish is manually taken spermary operation.
4) it is fertilized
By the spermary collected mortar grinder pulp, the ovum of extrusion is subsequently adding, add water activation, and ceaselessly stirs
Dynamic water a few minutes, form germ cell；
5) hatch
Germ cell is layered on and is made in the hatching frame of 70cm*40cm*5cm with the screen cloth of batten and 30 mesh, and frame will be hatched
It is placed in hatchery.Hatchery control intensity of illumination is covered with double-deck sun net.Hatching plate keeps flat, one in the middle of two blocks of hatching plates
Gas exhausting stone aeration.Water temperature is controlled at 24～27 degree simultaneously, is changed water daily once, is changed water, germ cell membrane using micro- pipeline mode
Shi Yitian changes water twice, equally changes water using micro- pipeline mode, and changes water half every time, it is ensured that the oxygen content in pond.
Behind three days of fertilization, absorb to hatching the dead ovum in frame.Hereafter, absorb daily the dead ovum of inframe with it is dead
Seedling, and micro- pipeline mode changes water once.
6) temporarily support and cultivate
Temporarily supported after fry membrane, changed water daily once, and water oxygen supplement is stirred with gas exhausting stone inflation, until fry vigor increases
By force.Then the fry of artificial propagation is put in the culture pond for having cultivated zooplankton.When fry grows to 4cm～more than 8cm,
The soft particulate material of 40% (weight) protein content being processed into together with powder with fresh minced fish is fed to which, day feeding volume is body
The 2% of weight.
Embodiment 2
In the present embodiment, from embodiment 1 1) to unlike 6), step 5) in, germ cell is placed in hatching barrel and is flowed
Water is hatched.
The above, the only specific embodiment of the present invention, but protection scope of the present invention is not limited thereto, any
Those familiar with the art the invention discloses technical scope in, change or replacement can be readily occurred in, should all be contained
Cover within protection scope of the present invention.Therefore, protection scope of the present invention described should be defined by scope of the claims.
Claims (9)
1. a kind of artificial fecundation method of big fin Of-digestive-tract, it is characterised in that comprise the following steps：
1) big fin Of-digestive-tract is fed, if wild big fin Of-digestive-tract, needs to carry out nursing domestication, wild fry is with 1.5～2%
The saline soak of (weight) entered pond after 3～5 minutes, feeds Limnodrilus hoffmeisteri within 5～15 days before entering pond, which is fed after 15 days and use fresh fish
Soft particulate material of the rotten and protein content made by powder for 40% (weight)；It is if artificial culture fry out, directly right
Which feeds the soft particulate material of 40% (weight) protein content being processed into together with powder with fresh minced fish；Day feeding volume is body weight
0.1%～4%, when water temperature is less than 18 degree, reduce feeding volume, less than 12 degree do not feed, and daily timing is washed by water, unlatching increasing
Oxygen machine oxygen supplement, monthly regularly changes water, and until which reaches sexual maturity, the ovary of raun reaches fourth stage, III phase of milter gonad development；
2) in spawning pond, 25～33 degree of water temperature is controlled, to big fin Of-digestive-tract manual injection's oxytocic hormones, after being spaced 12～15 hours,
Oxytocic hormone is injected again；
3) raun is carried out squeezing ovum in 20 hours after injecting at second, spermary handss is manually taken to the milter
Art；
4) by the spermary collected mortar grinder pulp, the ovum of extrusion is subsequently adding, add water activation, and ceaselessly stirs
Water a few minutes, germ cell is layered in hatchery and is hatched；
5) in hatching process, hatchery control intensity of illumination is covered with double-deck sun net, and add gas exhausting stone aeration oxygen replenishing to incubate
Change, while water temperature is controlled at 24～27 degree, change water daily once, change water twice within one day during germ cell membrane, change water half every time；
6) temporarily supported after fry membrane, changed water daily once, and water oxygen supplement is stirred with gas exhausting stone inflation, until fry vigor increases
By force.
2. the artificial fecundation method of big fin Of-digestive-tract according to claim 1, it is characterised in that：In step 6) in, by artificial breeding
Grow the fry for be put in the culture pond for having cultivated zooplankton, when fry grows to 4cm～8cm, which is fed and uses fresh fish
The rotten soft particulate material being processed into together with the powder that protein content made by powder is 40% (weight), day feeding volume are body weight
0.1%～4%.
3. the artificial fecundation method of big fin Of-digestive-tract according to claim 2, it is characterised in that：In step 1) in wild big fin
When Of-digestive-tract carries out feeding domestication process, the day feeding volume for body weight 2% is prevented to the big fin Of-digestive-tract for having wound using chlorine dioxide
Control, while irregularly carry out parasite killing using the chlorinated product of strong chlorine oil and DBDMH, net protozoon, metrifonate and avilamycin preventing
Control.
4. the artificial fecundation method of big fin Of-digestive-tract according to claim 1 and 2, it is characterised in that：In step 2) inject and hasten parturition
In the parent of hormone, raun weight is 220～270 grams, and milter weight is 400～500 grams, and the ratio of the female milter quantity is
3～5:1.
5. the artificial fecundation method according to the arbitrary described big fin Of-digestive-tract of claim 1-3, it is characterised in that：In step 2) in, institute
The oxytocic hormone stated be LHRH-A2, DOM, HCG composition mixture, dosage be per kilogram fish injection amount be respectively 20～
40ug, 20～40mg, 3000～6000IU.
6. the artificial fecundation method of big fin Of-digestive-tract according to claim 4, it is characterised in that：In step 2) in, spawning pond is
Flowing water spawning pond, carries out soaking lixiviating three times to pond, and allows the long periphyton of pool wall.
7. the artificial fecundation method of big fin Of-digestive-tract according to claim 5, it is characterised in that：The oxytocic hormone agent of the raun
Measure the proportions according to every kilogram of 40ug, 40mg, 6000IU for LHRH-A2, DOM, HCG, the oxytocic hormone agent of the milter
Measure the proportions according to every kilogram of 20ug, 20mg, 3000IU for LHRH-A2, DOM, HCG.
8. the artificial fecundation method according to the arbitrary described big fin Of-digestive-tract of claim 1-3, it is characterised in that：In step 4) in, institute
The hatching frame that the screen cloth of setting batten and 30 mesh in hatchery is made is stated, germ cell is layered on hatching frame.
9. the artificial fecundation method of big fin Of-digestive-tract according to claim 8, it is characterised in that：In step 5) hatching of germ cell
During, dead ovum to be absorbed, after fertilization starts on the 4th day, absorbs dead ovum and dead seedling daily, and changes water once.
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| CN201410675074.5A CN104488760B (en) | 2014-11-21 | 2014-11-21 | Artificial propagation method for mystus macropterus |
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| CN104488760B true CN104488760B (en) | 2017-04-12 |
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| CN105248322A (en) * | 2015-10-14 | 2016-01-20 | 南宁学院 | Artificial seedling growing method of mystus guttatus |
| CN111587818B (en) * | 2020-05-27 | 2021-12-28 | 武汉中科瑞华生态科技股份有限公司 | Artificial propagation method of mystus |
| CN112243895B (en) * | 2020-10-15 | 2022-04-15 | 日照市海洋水产资源增殖有限公司 | Efficient artificial spawning induction method for female cynoglossus semilaevis |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103283657A (en) * | 2013-06-09 | 2013-09-11 | 官广青 | Culture method of mystus macropterus |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103283657A (en) * | 2013-06-09 | 2013-09-11 | 官广青 | Culture method of mystus macropterus |
Non-Patent Citations (4)
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| 大鳍鳠的人工繁殖与苗种培育研究;马跃岗等;《重庆水产》;20120630(第2期);第1.4-1.5节 * |
| 大鳍鳠的生物学特性和人工繁养殖前景研究;朱成科等;《水利渔业》;20070531;第27卷(第5期);全文 * |
| 大鳍鳠的繁殖生物学研究;王德寿等;《水产学报》;19920331;第16卷(第1期);全文 * |
| 长江大鳍鳠的人工繁殖;杨德国等;《中国水产科学》;19980630;第5卷(第2期);第1.1-3.3节 * |
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