CN109511579A - The artificial propagation techniques of sharp naked carp - Google Patents

Abstract

The invention discloses a kind of artificial propagation techniques of sharp naked carp, comprising the following steps: S1) collection of wild parent population；S2) wild parent population is raised and train；S3) parent fish rearing；S4) Juvenile stage；S5) artificial induced spawning: being hastened parturition using biphasic injection, and injection only injects raun for the first time, is injected respectively after 36 hours to raun and milter, and wherein the amount of the ocyodinic of the injection of milter is the 1/2 of the amount of the ocyodinic of raun injection；S6) artificial insemination；S7) indoor hatching.The invention discloses a kind of artificial propagation techniques of sharp naked carp; parent population acclimatization culture and the artificial propagation of sharp naked carp are realized for the first time; basis is provided for the artificial culture of sharp naked carp, while important technical guarantee is also provided to carry out species conservation by domestication's ectogenesis for endangered species point naked carp.

Description

The artificial propagation techniques of sharp naked carp

Technical field

The present invention relates to aquatic products artificial propagation techniques fields, in particular to the artificial propagation techniques of sharp naked carp.

Background technique

Sharp naked carp (scientific name: Oxygymnocypris stewarti) is the fish that Cypriniformes Cyprinidae splits abdomen subfamily point Gymnocypris Class, popular name Si Shi naked carp fish, is locally commonly called as " whitefish ".In China, Tibet Yarlung Zangbo River middle reaches etc. are distributed in, the Heavenly Stems and Earthly Branches are more common in Flow flowing water water body.The type locality of the species is in Nyang Qu rivers.For China endemic species, it is only distributed in Tibet Yarlung Zangbo River middle reaches. Sharp naked carp is autonomous region first class of protection animal, is the distinctive fingerling in imminent danger in the Yarlung Zangbo River.

Breeding is an important turning point in fish whole life cycle, energy after the sexal maturity time of fish and sexal maturity The features such as the distribution mechanism of amount together constitute its Reproductive Strategy, the Reproductive Strategy of fish generally determine its gonad development, at A series of breeding activities such as ripe and oviposition behavior.The fish inhabited in specific bodies of water environment pass through long-term natural selection, The Reproductive Strategy being adapted with its specific habitat environment is formd, this strategy guarantees kind and its offspring to living environment There is maximum adaptability.

As cold water fishes, summer and autumn point naked carp is liked inhabiting (not average by the shallow water area of slack water of direct sunlight 15.1 DEG C of water temperature, 1~3m of the depth of water)；And winter-spring season like inhabiting the ice-bound profundal zone in water body surface layer (1.0 DEG C of average temperature, 4~5m of the depth of water).Based on gravel, this geology provides the geology of sharp naked carp habitat for the hatching of its sinking property fertilized eggs Refuge.Further, since the primary productivity in Yarlung Zangbo River waters is lower, this makes the food resource of sharp naked carp more deficient It is weary.

The annual March is the peak period of sharp naked carp oviposition, and spawning ground main feature may are as follows: is located at height above sea level 4000m Above Yarlung Zangbo River Lazi County to Angren County section, inhabits shoaling layer during oviposition, water quality is limpid, and substrate is mostly goose ovum Stone, silt is less, and oviposition waters bank is mostly stone and cobblestone.Sharp naked carp produces demersal egg, is in yellow, and ovum diameter 1.59mm~ 2.74mm, water swelling after ovum fertilization, hatches in crack of stone.

Summary of the invention

The purpose of the present invention is to provide a kind of artificial propagation techniques of sharp naked carp, realize the artificial propagation of sharp naked carp, drop The low risk for leading to species extinction because of the variation of living environment due to sharp naked carp.

The present invention is achieved through the following technical solutions: the artificial propagation techniques of sharp naked carp, comprising the following steps:

S1) the collection of wild parent population: selecting that constitution is strong, injured lighter wild parent population, and low-temperature transport is to fish farm after disinfection；

S2) wild parent population is raised and train: being raised and train using bait small fish and man-made feeds；

S3) parent fish rearing: selection is put into running water technology in outdoor fishpond by the wild parent population raised and train, alternately close Fish；

S4) Juvenile stage: the selected reproduction parent population from alternative parent population is put into storage pond, holding pond, increases daily bait injected volume, Continue flowing water culture；

S5) artificial induced spawning: being hastened parturition using biphasic injection, and injection only injects raun for the first time, right after 36 hours Raun and milter are injected respectively, and wherein the amount of the ocyodinic of the injection of milter is the 1/2 of the amount of the ocyodinic of raun injection；

S6) artificial insemination: when the closing effect time, checking raun and press its abdomen, if the smooth outflow of fish-egg energy, that is, open The water with parent population is dried in beginning artificial insemination, is squeezed into a clean container ovum under the conditions of being protected from light, is then squeezed into again Sperm, and container is shaken, sperm and ovum are come into full contact with, a small amount of clear water is added and stirs 1 minute, complete insemination；

S7) indoor hatching: fertilized eggs are placed in incubator, Lotic hatching；

Wherein step S5) in, the ocyodinic of injection is 2~3mg/kg+LRH-A2 of Dom, 1~2ug/kg+ for the first time 500~650IU/kg of HCG500~650IU/kg or PG 2~3mg/kg+LRH-A2,1~2ug/kg+HCG；Second of injection Ocyodinic are as follows: 4~6mg/kg+ of Dom 4~6mg/kg+LRH-A2 3~4ug/kg+HCG 800~1000IU/kg or PG 800~1000IU/kg of LRH-A23~4ug/kg+HCG.

In breeding season, raun abdomen expands, flexible, and gonopore is micro- red slightly prominent, milter gonopore invagination.It is general to select Body colour is preferable, physical strong, the preferable parent population of maturity.When artificial induced spawning, injected using body cavity, pectoral fin base portion without At squama, syringe needle is inserted into 0.3~0.5 centimetre at 40~60 degree of angles towards fish head direction and body axis, is slowly injected into ocyodinic.It has injected Breeding parent population after is put into spawning pond.Preferably, when injection time first time for breeding parent population is afternoon 5~7, to control The egg-laying time of system breeding parent population is morning.

The milter is identical with the injecting method of raun, and the formula of ocyodinic is identical, but injection volume is second of note of raun The 1/2 of the injection volume penetrated.

Further, step S1) specifically include that screening constitution is strong, injured lighter wild parent population, with penicillin and food Salt mixed aqueous solution impregnates one minute, is put into parent population bag, is filled with oxygen, transports under low-temperature condition；It is put behind transport to fish farm Enter and raises and train in pond wait raise and train；The transport temperature of wild parent population is 10~15 DEG C, and cool-down method uses and is put into frost in fish bag Ice bag.

Low-temperature condition can reduce the metabolism of sharp naked carp parent population, and water quality is made to be not easy to degenerate, and can also reduce fish to oxygen Consumption makes wild parent population be not easy the sick, condition of the injury during transportation and aggravates, reduces the death of wild parent population.

Further, the step S1) in, wild parent population is packed into before parent population bag, and 800,000 units are added in parent population bag Penicillin, carry out disinfection sterilization to the water in parent population bag and parent population bag, avoid wild parent population sick during transportation；Together When, also there is certain bactericidal anti-inflammatory effect to the wild parent population per se with wound, making it, the condition of the injury does not aggravate during transportation, Improve survival rate.

Further, the step S2) it is specifically included in and is put into raising and train in pond for wild parent population and is put by disinfection The quantity ratio of bait small fish, bait small fish and wild parent population is 1:20, is searched for food and is finished to bait small fish, and timing puts into artificial raise Material, and daily pull the man-made feeds that do not search for food out, continue 4~5 months, completes to raise and train.

Further, the step S2) in, man-made feeds include Microparticle diets and water earthworm.

Further, the Microparticle diets include following component: fish meal, dregs of beans, soy protein concentrate, cereal, Yeast cell wall, refined fish oil, calcium dihydrogen phosphate, sodium chloride, vitamin, microelement, mould inhibitor.

During the parent fish rearing, alternative parent population cultivates feed using early period, using in above-mentioned Microparticle diets Each components matching form, early period cultivate feed in crude protein >=40%, coarse ash≤16%, crude fibre≤6.0%, crude fat >=4.5%, total phosphorus >=1.2%, lysine >=2.0%, moisture≤12.5.The breeding parent fish rearing picked out in alternative parent population When, using antenatal reinforced cultivating feed, the ingredient of term reinforced cultivating feed is consistent with feed ingredient is cultivated early period, but its component Difference, by adjusting fish meal, dregs of beans, soy protein concentrate, cereal, yeast cell wall, refined fish oil, calcium dihydrogen phosphate, chlorination Sodium, vitamin, the ratio between microelement, obtain crude protein >=55%, coarse ash≤18%, crude fibre≤4.0%, thick rouge Fat >=4.5%, total phosphorus >=1.5%, lysine >=2.9%, the high protein feed of moisture≤12.5.

Further, the step S3) it is specifically included in by selection body surface in the wild parent population raised and train without wound, weight 0.75kg or more milter and 1.5kg or more raun alternately parent population, alternative parent population is thrown and uses running water technology in outdoor fishpond, It is 100 tails/12m that it, which cultivates density,³；Daily dispensing man-made feeds are primary, and every day feeding volume is the 1~2% of alternative parent population weight, Feed artifical compound feed twice daily.

Further, the step S3) in, the inflow in the fishpond of alternative parent population is 0.6m/s, cultivates the pH for using water For 6.8~8.2, dissolved oxygen be 10~12mg/L, water temperature is 12-17 DEG C.

Further, the step S4) it is specifically included in and screens mature alternative parent population in alternative parent population as breeding parent Fish, wherein raun selection abdomen expands, is soft, flexible, is gently pressed with sense of movement, the micro- red individual of gonopore；Milter selection Body is active, and figure is slightly smaller, and the styloid individual of gonopore, the ratio of the raun and milter that select is 1:2.5；Breeding parent population is put Enter in storage pond, holding pond, and carry out running water technology, increase the flow velocity of water flow, daily bait injected volume be breed parent population weight 3~ 5%, artifical compound feed is fed twice daily.

Further, the step S4) in breeding parent population storage pond, holding pond in inflow be 1.5m/s, increase flowing water to numerous The effect of stimulation of parent population is grown, the pH of cultivation water is 6.8~8.2, dissolved oxygen is 10~12mg/L, water temperature is 13.0 ± 0.5 DEG C.

Since sharp naked carp keeps its temperature requirement higher breeding, temperature need to be constant at 13.0 ± 0.5 DEG C, and temperature is excessively high Or too low the spawning rate of sharp naked carp and the hatching rate of fertilized eggs can be produced bigger effect.

Further, the step S7) in, the quantity of circulating water per hour in incubator is in 50m3, incubator size 60cm* 80cm*3cm controls 10~12 DEG C of water temperature, and dissolved oxygen is controlled in 4mg/L or more, pH7.0~8.0.

Compared with prior art, the present invention have the following advantages that and the utility model has the advantages that

(1) artificial propagation techniques of a kind of sharp naked carp disclosed by the invention realize the parent population domestication training of sharp naked carp for the first time It educates and artificial propagation, the artificial culture for sharp naked carp provides basis, while also passing through domestication's training for endangered species point naked carp It educates breeding and provides important technical guarantee to carry out species conservation.

(2) artificial propagation techniques of a kind of sharp naked carp disclosed by the invention, carry out people to sharp naked carp using biphasic injection Work is hastened parturition, and spawning rate is high, and efficiency of hastening parturition is up to 93%.

(3) in a kind of artificial propagation techniques of sharp naked carp disclosed by the invention, the domestication survival rate of wild parent population is high, fish-egg Hatching rate can reach 62.54%.

Specific embodiment

The embodiment of the present invention is described below in detail.The embodiments described below is exemplary, it is intended to for explaining this Invention, and be not considered as limiting the invention.

When wild parent population is collected, wild parent population resource investigation is carried out based on the sex investigation of region by visiting local fisherman, Fixed sample section is not set, is set according to river section situation and is faced times transforming acquisition fishes snd.

The gonad development and maturation of parent population have apparent stage, and therefore, as shown in table 1, the development of sexual gland is divided into Following six period, and alternative parent population and breeding parent population are selected according to the characteristics of gonad development.

Table 1

Sharp naked carp is of short duration to be in for VI phase after oviposition, and hereafter 5-7 month ovary was returned to for III phase, finally develops to IV phase It passes the winter.Spermary rested on for III-IV phase after breeding period, and in the present embodiment, the sharp naked carp that sexual gland was in for III-VI phase is defined To have reached sexal maturity individual.

Embodiment 1

The artificial propagation techniques of sharp naked carp, the artificial propagation techniques of sharp naked carp, comprising the following steps:

S1) the collection of wild parent population: screening constitution is strong, injured lighter wild parent population, is mixed with penicillin and salt It aqueous solution soaking one minute, is put into parent population bag, is filled with oxygen, is transported under low-temperature condition；It is put into and raises and train behind transport to fish farm Wait raise and train in pond；Wild parent population is packed into before parent population bag, and the penicillin of 800,000 units is added in parent population bag, to parent population bag and parent Water in fish bag carries out disinfection sterilization, avoids wild parent population sick during transportation；Meanwhile to per se with the wild of wound Parent population also has certain bactericidal anti-inflammatory effect, and making it, the condition of the injury does not aggravate during transportation, improves survival rate.

In the wild parent population of collection, whose body weight reaches the individual of 1kg or more, is analyzed according to anatomy, these fishes Most of gonad development reaches parent population specification to IV, so parent population is raised and train and cultivates while carrying out.

S2) parent population is raised and train: raising and train the bait small fish being put into pond by disinfection be put into wild parent population, bait is small The quantity ratio of fish and wild parent population is 1:20, is searched for food and is finished to bait small fish, and timing puts into man-made feeds, and will not adopt daily The man-made feeds of food are pulled out, are continued 4~5 months, are completed to raise and train；Man-made feeds include Microparticle diets and water earthworm.Institute Stating Microparticle diets includes following component: fish meal, dregs of beans, soy protein concentrate, cereal, yeast cell wall, refined fish oil, Calcium dihydrogen phosphate, sodium chloride, vitamin, microelement, mould inhibitor.

S3) parent fish rearing: through selection body surface in wild parent population that raising and train without wound, the above milter of weight 0.75kg and Alternative parent population is thrown and uses running water technology in outdoor fishpond by 1.5kg or more raun alternately parent population, and cultivating density is 100 Tail/12m³；Daily dispensing man-made feeds are primary, and every day feeding volume is the 1~2% of alternative parent population weight, feeds manually match daily Close feed twice；

During the parent fish rearing, alternative parent population cultivates feed using early period, using in above-mentioned Microparticle diets Each components matching form, early period cultivate feed in crude protein >=40%, coarse ash≤16%, crude fibre≤6.0%, crude fat >=4.5%, total phosphorus >=1.2%, lysine >=2.0%, moisture≤12.5.The breeding parent fish rearing picked out in alternative parent population When, using antenatal reinforced cultivating feed, the ingredient of term reinforced cultivating feed is consistent with feed ingredient is cultivated early period, but its component Difference, by adjusting fish meal, dregs of beans, soy protein concentrate, cereal, yeast cell wall, refined fish oil, calcium dihydrogen phosphate, chlorination Sodium, vitamin, the ratio between microelement, obtain crude protein >=55%, coarse ash≤18%, crude fibre≤4.0%, thick rouge Fat >=4.5%, total phosphorus >=1.5%, lysine >=2.9%, the high protein feed of moisture≤12.5.

Inflow in the fishpond of alternative parent population is 0.6m/s, the pH of cultivation water is 6.8~8.2, dissolved oxygen be 10~ 12mg/L, water temperature are 12-17 DEG C.

S4) Juvenile stage: the alternative parent population of 80 tails maturation is screened in alternative parent population as breeding parent population, wherein raun selects It selects abdomen expand, is soft, flexible, is gently pressed with sense of movement, the micro- red individual of gonopore；Milter selection individual is active, and figure is slightly Small, the styloid individual of gonopore, the ratio of the raun and milter that select is 1:2.5；Breeding parent population is put into storage pond, holding pond, and Running water technology is carried out, the flow velocity of water flow is increased, daily bait injected volume is breed parent population weight 3~5%, artifical compound feed It feeds twice daily.Breeding the inflow in the storage pond, holding pond of parent population is 1.5m/s, and cultivating with the pH of water is 6.8~8.2, dissolved oxygen It is 13.0 ± 0.5 DEG C for 10~12mg/L, water temperature.

S5) artificial induced spawning: being hastened parturition using biphasic injection, and injection only injects raun for the first time, right after 36 hours Raun and milter are injected respectively, and wherein the amount of the ocyodinic of the injection of milter is the 1/2 of the amount of the ocyodinic of raun injection； Ocyodinic has pituitary (PG) luteotropin releasing hormone d-ala analog (LRH-A), maleic acid dioxone (DOM) and chorion rush property Glandular hormone (HCG).

The ocyodinic of injection is Dom 2mg/kg+LRH-A2 2ug/kg+HCG 500IU/kg, Effect time for the first time It is 36 hours；The ocyodinic of second of injection are as follows: Dom 5mg/kg+LRH-A2 3ug/kg+HCG 800IU/kg (please be supplemented and be urged Produce the injection volume of agent), Effect time is 12 hours.

In breeding season, raun abdomen expands, flexible, and gonopore is micro- red slightly prominent, milter gonopore invagination.It is general to select Body colour is preferable, physical strong, the preferable parent population of maturity.When artificial induced spawning, injected using body cavity, pectoral fin base portion without At squama, syringe needle is inserted into 0.3~0.5cm at 40~60 ° of angles towards fish head direction and body axis, is slowly injected into ocyodinic.Preferably, needle The body axis in head towards fish head direction and breeding parent population is inserted into 0.4cm in 50~55 ° of angles.Breeding parent population after the completion of injection is put into oviposition Chi Zhong.Preferably, when injection time first time for breeding parent population is afternoon 5~7, it is to control the egg-laying time of breeding parent population Morning.

S6) artificial insemination: when the closing effect time, checking raun and press its abdomen, if the smooth outflow of fish-egg energy, that is, open The water with parent population is dried in beginning artificial insemination, is squeezed into a clean container ovum under the conditions of being protected from light, is then squeezed into again Sperm, and container is shaken, sperm and ovum are come into full contact with, a small amount of clear water is added and stirs 1 minute, complete insemination；

S7) indoor hatching: fertilized eggs are placed in incubator, Lotic hatching.Quantity of circulating water per hour in incubator exists 50m3, incubator size 60cm*80cm*3cm control 10~12 DEG C of water temperature, dissolved oxygen control in 4mg/L or more, pH7.0~ 8.0.In hatching process, it is protected from light hatching.

Embodiment 2:

The present embodiment is further improved on the basis of embodiment 1, is theed improvement is that: in step S5), first The ocyodinic of secondary injection is PG 2mg/kg+LRH-A2 2ug/kg+HCG 500IU/kg；Its Effect time is 36 hours；Second The ocyodinic of secondary injection are as follows: PG 5mg/kg+LRH-A2 3ug/kg+HCG 800IU/kg, Effect time are 12 hours.

The present embodiment rest part is substantially the same manner as Example 1, no longer repeats one by one.

Embodiment 3:

The present embodiment is further improved on the basis of embodiment 1, is theed improvement is that: in step S5), first The ocyodinic of secondary injection is PG 3mg/kg+LRH-A2 1ug/kg+HCG 650IU/kg；Its Effect time is 36 hours；Second The ocyodinic of secondary injection are as follows: PG 4mg/kg+LRH-A2 4ug/kg+HCG 1000IU/kg, Effect time are 12 hours.

The present embodiment rest part is substantially the same manner as Example 1, no longer repeats one by one.

Embodiment 4:

The present embodiment is further improved on the basis of embodiment 1, is theed improvement is that: in step S5), first The ocyodinic of secondary injection is PG 3mg/kg+LRH-A2 1ug/kg+HCG 650IU/kg；Its Effect time is 36 hours；Second The ocyodinic of secondary injection are as follows: PG 4mg/kg+LRH-A2 4ug/kg+HCG 1000IU/kg, Effect time are 12 hours.

The present embodiment rest part is substantially the same manner as Example 1, no longer repeats one by one.

Comparative example 1

It is added without bait small fish as bait using Microparticle diets and water earthworm always, other steps and implementation Step S2 in example 1) it is identical.When 5d, the small fish quantity in pond of raising and train of embodiment is 17 tails, and has no small fish corpse, is tentatively sentenced Break to be searched for food by wild parent population；7d small fish quantity is 5 tails, has no small fish corpse, determines that small fish is preyed on by parent population, parent population starts Restore constitution, mental alertness；8d small fish quantity is 0 tail, starts to feed 2.0 artifical compound feeds, and wild parent population does not search for food feeding Material；It pulls feed of not searching for food out daily, feeds quantitative mixed feed, 13d has individual wild parent populations to start man-made feeds of searching for food, adopts Appetite reduces；It persistently feeds, wild feeding is normal after 20d, and growing state is good.Comparative example 1 is raised and train in pond, wild parent population Always do not occur obviously feeding, constitution is poor, and figure is thin, domestication failure.

Comparative example 2

Choose through step S1 in embodiment 1)~step S4) in breeding parent population, using isotope labeled leucine to breeding parent Fish carries out artificial induced spawning, and the ocyodinic of injection is DOM 7mg/kg+LRH-A2 5ug/kg+HCG 1300IU/kg, milter Injection volume and raun injection volume it is same as Example 1.The present embodiment rest part is substantially the same manner as Example 1, not further One repeats.

First day interval checks primary that every 6 hours check once after second day, have at the 60th hour for every 12 hours after injection Individual rauns collect mature ovum.

Hastening parturition for embodiment and comparative example is as shown in table 2 with hatch results.

Serial number

Parent population quantity

Gross weight

It hastens parturition success

Collect fertilized eggs

Spawning rate

Egg laying amount/kg

Hatching rate

Embodiment 1

30 tails

49.3kg

27 tails

238050 pieces

90.00%

4828 pieces

62.63%

Embodiment 2

30 tails

47.6kg

28 tails

244160 pieces

93.33%

5129 pieces

62.54%

Comparative example 2

30 tails

50.5kg

8 tails

69200 pieces

26.67%

1370 pieces

63.55%

Table 2

As shown in table 2, under mantissa's same case of hastening parturition, using spawning rate the urging far below biphasic injection of one injection Yield, and fertilized eggs, the egg laying amount being collected into also are far below the spawning rate of biphasic injection, the spray fish being collected into embodiment 1 Seedling number is 149091 tails, and the hatching rate of fertilized eggs is 62.63%, and the spray fry number that embodiment 2 is collected into is 152700 tails, The hatching rate of its fertilized eggs is 63.54%；The spray fry number that comparative example 2 is collected into is 43980 tails, the hatching rate of fertilized eggs It is 63.55%, is not much different with embodiment 1 and embodiment 2.It is biggish in the fertilized eggs quantity difference that spawning rate and collection reach In the case of, it is hastened parturition using biphasic injection more preferable to the spawning effect of sharp naked carp, the artificial propagation of more conducively sharp naked carp.Together When, using PG+LRH-A2+HCG as ocyodinic and DOM+LRH-A2+HCG as ocyodinic, spawning effect is suitable, equal energy Reach preferable spawning effect.

The above is only presently preferred embodiments of the present invention, not does limitation in any form to the present invention, it is all according to According to technical spirit any simple modification to the above embodiments of the invention, equivalent variations, protection of the invention is each fallen within Range.

Claims (9)

1. the artificial propagation techniques of sharp naked carp, the artificial propagation for sharp naked carp, it is characterised in that: the following steps are included:

S1) the collection of wild parent population: selecting that constitution is strong, injured lighter wild parent population, and low-temperature transport is to fish farm after disinfection；

S2) wild parent population is raised and train: being raised and train using bait small fish and man-made feeds；

S3) parent fish rearing: selection is put into running water technology in outdoor fishpond, alternately parent population by the wild parent population raised and train；

S4) Juvenile stage: the selected reproduction parent population from alternative parent population is put into storage pond, holding pond, increases daily bait injected volume, continues Carry out flowing water culture；

S5) artificial induced spawning: being hastened parturition using biphasic injection, and injection only injects raun for the first time, to raun after 36 hours It is injected respectively with milter, wherein the amount of the ocyodinic of the injection of milter is the 1/2 of the amount of the ocyodinic of raun injection；

S6) artificial insemination: when the closing effect time, checking raun and press its abdomen, if the smooth outflow of fish-egg energy, that is, start people Work insemination, dries the water with parent population, squeezes into a clean container ovum under the conditions of being protected from light, then squeeze into essence again Liquid, and container is shaken, sperm and ovum are come into full contact with, a small amount of clear water is added and stirs 1 minute, complete insemination；

S7) indoor hatching: fertilized eggs are placed in incubator, Lotic hatching；

Wherein step S5) in, for the first time injection ocyodinic be 2 ~ 3mg/kg+LRH-A2 of Dom, 1 ~ 2ug/kg+HCG 500 ~ 500 ~ 650IU/kg of 650IU/kg or PG 2 ~ 3mg/kg+LRH-A2,1 ~ 2ug/kg+HCG；The ocyodinic of second of injection are as follows: 3 ~ 4ug of Dom 4 ~ 6mg/kg+LRH-A2,3 ~ 4ug/kg+HCG 800 ~ 1000IU/kg or PG 4 ~ 6mg/kg+ LRH-A2/kg +HCG 800~1000IU/kg。

2. the artificial propagation techniques of point naked carp according to claim 1, it is characterised in that: the step S1) it specifically includes It screens that constitution is strong, injured lighter wild parent population, is impregnated one minute with penicillin and salt mixed aqueous solution, be put into parent population bag In, it is filled with oxygen, is transported under low-temperature condition；It is put into and is raised and train in pond wait raise and train behind transport to fish farm；The transport temperature of wild parent population Degree is 10 ~ 15 DEG C, and cool-down method uses the ice bag that frost is put into fish bag.

3. the artificial propagation techniques of point naked carp according to claim 2, it is characterised in that: the step S1) in, wild parent Fish is packed into before parent population bag, and the penicillin of 800,000 units is added in parent population bag.

4. the artificial propagation techniques of point naked carp according to claim 1, it is characterised in that: the step S2) it specifically includes In the bait small fish being put into pond by disinfection of raising and train for being put into wild parent population, bait small fish and the quantity ratio of wild parent population are 1:20 is searched for food to bait small fish and is finished, and timing puts into man-made feeds, and daily pulls the man-made feeds that do not search for food out, continues 4 It ~ 5 months, completes to raise and train.

5. the artificial propagation techniques of point naked carp according to claim 1, it is characterised in that: the step S3) it specifically includes Through selection body surface in wild parent population that raising and train without wound, the above milter of weight 0.75kg and 1.5kg or more raun alternately Alternative parent population is thrown and uses running water technology in outdoor fishpond by parent population, and cultivation density is 100 tails/12m³；It is daily to launch artificial raise Material is primary, and every day feeding volume is the 1 ~ 2% of alternative parent population weight, feeds artifical compound feed daily twice.

6. the artificial propagation techniques of point naked carp according to claim 5, it is characterised in that: the step S3) in, alternative parent Inflow in the fishpond of fish is 0.6m/s, and it is 10 ~ 12mg/L, water temperature 12- that the pH of cultivation water, which is 6.8 ~ 8.2, dissolved oxygen, 17℃。

7. the artificial propagation techniques of point naked carp according to claim 1, it is characterised in that: the step S4) it specifically includes Mature alternative parent population is screened in alternative parent population as breeding parent population, wherein raun selection abdomen expands, is soft, flexible, Gently it is pressed with sense of movement, the micro- red individual of gonopore；Milter selection individual is active, and figure is slightly smaller, the styloid individual of gonopore, choosing The ratio of the raun and milter selected is 1:2.5；Breeding parent population is put into storage pond, holding pond, and carries out running water technology, increases water flow Flow velocity, daily bait injected volume are breed parent population weight 3 ~ 5%, and artifical compound feed is fed twice daily.

8. it is according to claim 7 point naked carp artificial propagation techniques, it is characterised in that: the step S4) in breeding parent Inflow in the storage pond, holding pond of fish is 1.5m/s, cultivate water pH be 6.8 ~ 8.2, dissolved oxygen be 10 ~ 12mg/L, water temperature is 13.0±0.5℃。

9. the artificial propagation techniques of point naked carp according to claim 1, it is characterised in that: the step S7) in, incubator In quantity of circulating water per hour in 50m³, 10~12 DEG C of water temperature are controlled, dissolved oxygen is controlled in 4mg/L or more, pH7.0~8.0.