CN110178767A - A kind of artificial fecundation method of point naked carp - Google Patents
A kind of artificial fecundation method of point naked carp Download PDFInfo
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- CN110178767A CN110178767A CN201910627176.2A CN201910627176A CN110178767A CN 110178767 A CN110178767 A CN 110178767A CN 201910627176 A CN201910627176 A CN 201910627176A CN 110178767 A CN110178767 A CN 110178767A
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- 241001677838 Cyprinus carpio nudus Species 0.000 title claims abstract description 43
- 238000000034 method Methods 0.000 title claims abstract description 33
- 241000251468 Actinopterygii Species 0.000 claims abstract description 32
- 230000012447 hatching Effects 0.000 claims abstract description 15
- 230000009027 insemination Effects 0.000 claims abstract description 7
- 238000002347 injection Methods 0.000 claims description 34
- 239000007924 injection Substances 0.000 claims description 34
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 26
- 108010000912 Egg Proteins Proteins 0.000 claims description 25
- 102000002322 Egg Proteins Human genes 0.000 claims description 25
- 210000004681 ovum Anatomy 0.000 claims description 25
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 18
- 239000000243 solution Substances 0.000 claims description 15
- 235000013601 eggs Nutrition 0.000 claims description 14
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 claims description 9
- 229930182566 Gentamicin Natural products 0.000 claims description 9
- 229960002518 gentamicin Drugs 0.000 claims description 9
- 239000011780 sodium chloride Substances 0.000 claims description 9
- 230000002051 biphasic effect Effects 0.000 claims description 7
- 230000000694 effects Effects 0.000 claims description 7
- 238000000386 microscopy Methods 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 6
- 210000003097 mucus Anatomy 0.000 claims description 5
- 238000002791 soaking Methods 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- 238000005516 engineering process Methods 0.000 claims description 4
- 239000008103 glucose Substances 0.000 claims description 4
- 230000001360 synchronised effect Effects 0.000 claims description 4
- 241000252233 Cyprinus carpio Species 0.000 claims description 3
- 238000013019 agitation Methods 0.000 claims description 3
- 239000000919 ceramic Substances 0.000 claims description 3
- 238000007689 inspection Methods 0.000 claims description 3
- 230000000366 juvenile effect Effects 0.000 claims description 3
- 210000001161 mammalian embryo Anatomy 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 230000001817 pituitary effect Effects 0.000 claims description 3
- 230000008569 process Effects 0.000 claims description 3
- 238000012797 qualification Methods 0.000 claims description 3
- 239000003488 releasing hormone Substances 0.000 claims description 3
- 230000003068 static effect Effects 0.000 claims description 3
- 238000011161 development Methods 0.000 abstract description 2
- 230000018109 developmental process Effects 0.000 abstract description 2
- 230000004720 fertilization Effects 0.000 description 5
- 230000032696 parturition Effects 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 2
- 230000002196 ecbolic effect Effects 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000003204 osmotic effect Effects 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 230000001850 reproductive effect Effects 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 241000252210 Cyprinidae Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241001275923 Gymnocypris Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 206010021143 Hypoxia Diseases 0.000 description 1
- 241001365789 Oenanthe crocata Species 0.000 description 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 210000000436 anus Anatomy 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 210000001217 buttock Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 238000010835 comparative analysis Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000021060 food property Nutrition 0.000 description 1
- 230000007954 hypoxia Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011160 research Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/13—Prevention or treatment of fish diseases
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The present invention provides a kind of artificial fecundation methods of sharp naked carp, comprising the following steps: A, parent population selection；B, artificial induced spawning；C, artificial insemination；D, hatch.This method is remarkably improved the hatching rate and emergence rate of sharp naked carp, for protecting this Endemic fish and reasonable development its Stock resoures to be of great significance.
Description
Technical field
The present invention relates to the reproduction technique field of fish, especially a kind of artificial fecundation method of sharp naked carp.
Background technique
Sharp naked carp, is subordinate to Cyprinidae, Schizothoracinae, and sharp Gymnocypris is important one of the indigenous economic fish in Tibet.Point is naked
Carp belongs to the eggcase grade in schizothoracin class three grades monoid, be distributed only over 3600 meters of Yarlung Zangbo River middle reaches height above sea level with
Upper major dry tributary area.Since height above sea level, annual water temperature are low, special ecological environment is formed, sharp naked carp is adapted to drop
Water and high mountain snow-broth are source, and the limpider environment of water flow is mainly food with other fish and aquatic insect etc..In recent years, it is given birth to
The influence of state environment and mankind's activity, sharp naked carp inhabites to be deteriorated increasingly with living environment, and Yarlung Zangbo River step power station is repaired
Building compresses its living space greatly, causes its germ plasm resource amount sharply to reduce, has been put into Chinese animals on the brink of extinction
Red Data Book and Chinese vertebrate Red List.
Sharp naked carp fish belongs to plateau cold water fishes, and with slow growth, sexal maturity evening, reproductive capacity is low, resource supplement is all
The features such as phase is long, to the adaptation of living environment height and dependence, so that sharp naked carp is extremely sensitive to the variation of ecological environment, ecological ring
The change in border is larger to the procreation of its population and survival effect.Therefore, while protecting its habitat, artificial propagation is to increase
Its Natural Population quantity studies a kind of row generallyd use in the work of the species diversity conservations such as its reproductive biology and the history of life
Effective scientific and technical method, this be also the most of rare aquatic animals Natural Populations proliferation in current China with protect it is effective
One of approach.
Sharp naked carp is China's endemic species, and work on hand mainly includes qautobiology, muscle nutrition, Feeding organ and food
Property, biological community structure, resource investigation of fish and protection and chromosome multiformity, the comparative analysis of isodynamic enzyme, identified for genes
Etc., the research of more rare point naked carp artificial propagation techniques.
The patent of invention of publication number CN106577389A and CN102077787A, respectively disclose a kind of artificial propagation side of schizothoracin
Method, disclosed method include parent population selection and four cultivation, artificial induced spawning, artificial insemination and hatching steps.But artificial insemination side
Method has specific aim, and for different fishes, ocyodinic is also different, and incubation condition has specificity, therefore above method is not applicable
In the artificial propagation of sharp naked carp.
The patent of invention of notification number CN106879510B discloses a kind of naked induced spawning method for splitting buttocks fish in Lhasa, packet
Include: A, parent population selects；B, artificial induced spawning；C, artificial insemination；D, artificial incubation.Due to being directed to different fishes, oxytocic hormone and agent
Amount has differences, and the oxytocic hormone of the patent is not suitable for sharp naked carp.
Summary of the invention
Aiming at the problem that sharp naked carp inhabites and deteriorates increasingly with living environment, the invention proposes a kind of suitable for sharp naked carp
Artificial fecundation method, this method is remarkably improved the hatching rate and emergence rate of sharp naked carp, for protecting this Endemic fish and conjunction
Reason develops its Stock resoures and is of great significance.
The technical solution adopted by the present invention is that:
A kind of artificial fecundation method of point naked carp, comprising the following steps:
A, parent population selects: the April, and fishing developmental stage enters the wild sharp naked carp of IV phase as artificial propagation parent, to parent
After carrying out disinfection, separately female, milter is temporarily supported spare；
B, artificial induced spawning: luteotropin releasing hormone d-ala analog LRH-A is used2With carp pituitary PG mixture as ocyodinic,
LRH-A2Dosage be 10ug/kg, the dosage of PG mixture is 8mg/kg；Using biphasic injection, sharp naked carp raun is urged
It produces；
C, artificial insemination: being inseminated using anhydrous dry method, and raun passes through 48~72 hours Effect times, is manually squeezed to raun
Ovum is squeezed into sharp naked carp ovum grain in dry ceramic whiteware bowl, after pouring into the sperm of 3~4 drop milters, is sufficiently stirred 30 seconds, is added clear
Water stands 1 minute after gentle agitation；
Effect time 48~72 hours, started since raun injects the second needle, and Effect time is too short, and ovum grain does not dissociate,
It squeezes and does not come out, Effect time is too long, and ovum grain is overdone, and extruding most of is dead ovum.Stand be within 1 minute in order to allow ovum sufficiently by
Essence is to be fertilized since ovum touches that time of water just, the time is too short, and ovum fertilization rate is low, the time because dry method is fertilized
Too long, fertilized eggs will appear hypoxia death.
D, hatch: the fertilized eggs after standing are washed 2~3 times with clear water repeatedly, remove the mucus in fertilized eggs, then will
Fertilized eggs after unsticking are laid in hatching frame, are hatched；It in hatching process, is managed, is kept using miniflow water
Fertilized eggs remain static in hatching frame;The ovum not being fertilized and dead embryo are selected out with point on inspection；Water temperature 10~
Under conditions of 12 DEG C, after hatching 10~13 days, i.e., rupture of membranes becomes juvenile fish.
The invention also includes parent population postnatal cares: having adopted essence and has squeezed the parent population after ovum, has first been invaded with 1% glucose solution
Bubble 1 hour, allows it to regain one's strength, and then every fish injects gentamicin, and the parent population after injection is transferred to running water technology pond
In, lure it to ingest using small-sized cold water fishes as bait fish.
Postnatal care is that can be used as reserve parent fish to improve parent population postpartum survival rate, reduces the coming year to wild parent population
It relies on.
Preferably, the dosage of the injection gentamicin is 0.2mg/kg weight.The parent population in postpartum can generate by extruding
Wound, mucus fall off, and injection gentamicin can enhance fish body premunition, prevention and treatment water mold infection.
Parent population is impregnated 30 minutes with the NaCl solution of concentration 2~3% and is sterilized by step A of the present invention.
Different fishes is different to the tolerance of NaCl solution, adjustable by 2~3% NaCl solution soaking disinfection
Fish osmotic pressure supplements a part of salinity being lost because squeezing ovum damage, also can be relieved the stress reaction of fish.
Biphasic injection of the present invention are as follows: raun is injected 2 times, and injection accounts for the 20% of accumulated dose for the first time, and 24 hours
Afterwards, second of injection accounts for the 80% of accumulated dose；Milter is injected 1 time, and injection time is synchronous with second of injection time of raun, dosage
It is identical as second of injection dosage of raun.
Step C of the present invention before squeezing ovum to raun, first collects milter sperm and carries out microscopy, the sperm of microscopy qualification
It is kept in dark place at 4 DEG C spare.Microscopy is to determine sperm quality, and to improve rate of fertilization, sperm quality is good, and rate of fertilization is just
It is high.
The beneficial effects of the present invention are:
1, the artificial fecundation method is simple, strong operability, and practical application is good, may be implemented wild sharp naked carp scale and
Artificial propagation is standardized, the aquaculture development of Tibet point naked carp, effective protection and utilization Tibet point naked carp wild resource are promoted.
2, postnatal care is made to the parent population in postpartum, effectively improves parent population postpartum survival rate, can be used as reserve parent fish, reduce and
Dependence of the year to wild parent population；The parent population in postpartum can generate wound by extruding, and mucus falls off, and injection gentamicin can increase
Strong fish body premunition, prevention and treatment water mold infection.
3, it is impregnated by 2~3% NaCl solution and the parent population of fishing is sterilized, the osmotic pressure of adjustable wild sharp naked carp,
A part of salinity being lost because squeezing ovum damage is supplemented, also can be relieved the stress reaction of fish.
Specific embodiment
In order to it is clearer, explain purpose of the present invention technical solution in detail, below by related embodiment to this hair
It is bright to be described further.Following embodiment is only to illustrate implementation method of the invention, does not limit protection of the invention
Range.
Embodiment 1
A kind of artificial fecundation method of point naked carp, comprising the following steps:
A, parent population selects: the April, and fishing developmental stage enters the wild sharp naked carp of IV phase as artificial propagation parent, to parent
After carrying out disinfection, separately female, milter is temporarily supported spare；
B, artificial induced spawning: luteotropin releasing hormone d-ala analog LRH-A is used2With carp pituitary PG mixture as ocyodinic,
LRH-A2Dosage be 10ug/kg, the dosage of PG mixture is 8mg/kg；Using biphasic injection, sharp naked carp raun is urged
It produces；
C, artificial insemination: being inseminated using anhydrous dry method, and raun passes through 48~72 hours Effect times, is manually squeezed to raun
Ovum is squeezed into sharp naked carp ovum grain in dry ceramic whiteware bowl, after pouring into the sperm of 3~4 drop milters, is sufficiently stirred 30 seconds, is added clear
Water stands 1 minute after gentle agitation；
D, hatch: the fertilized eggs after standing are washed 2~3 times with clear water repeatedly, the mucus in fertilized eggs are removed, then by unsticking
Fertilized eggs afterwards are laid in hatching frame, are hatched；Hatch bolting silk mesh size 1.0mm in frame, in hatching process, uses
Miniflow water is managed, and fertilized eggs is kept to remain static in hatching frame;The ovum not being fertilized is selected out with point on inspection
And dead embryo；Under conditions of 10~12 DEG C of water temperature, after hatching 10~13 days, i.e., rupture of membranes becomes juvenile fish.
The wild sharp naked carp of IV phase: abdomen expands, is soft, has apparent ovary profile, anus is slightly prominent, rubescent, light to press
Postabdomen, cloacal aperture have the outflow of ovum grain.
Embodiment 2
The present embodiment is on the basis of embodiment 1:
Further include parent population postnatal care: having adopted essence and squeezed the parent population after ovum, first with glucose solution soaking 1 hour of 1%, so
Every fish injects gentamicin afterwards, and the parent population after injection is transferred in running water technology pond, using small-sized cold water fishes as
Bait fish lures it to ingest.
Embodiment 3
The present embodiment is on the basis of embodiment 1:
Further include parent population postnatal care: having adopted essence and squeezed the parent population after ovum, first with glucose solution soaking 1 hour of 1%, so
Every fish injects gentamicin afterwards, and the parent population after injection is transferred in running water technology pond, using small-sized cold water fishes as
Bait fish lures it to ingest.
The dosage of the injection gentamicin is 0.2mg/kg weight.
Embodiment 4
The present embodiment is on the basis of embodiment 1:
Parent population is impregnated 30 minutes with the NaCl solution of concentration 2~3% and is sterilized by the step A.
Embodiment 5
The present embodiment is on the basis of embodiment 1:
Parent population is impregnated 30 minutes with the NaCl solution of concentration 2~3% and is sterilized by the step A.
Biphasic injection are as follows: raun is injected 2 times, after injection accounts for the 20% of accumulated dose, 24 hours for the first time, second of injection
Account for the 80% of accumulated dose；Milter is injected 1 time, and injection time is synchronous with second of injection time of raun, second of note of dosage and raun
It is identical to penetrate dosage.
Embodiment 6
The present embodiment is on the basis of embodiment 1:
Parent population is impregnated 30 minutes with the NaCl solution of concentration 2~3% and is sterilized by the step A.
Biphasic injection are as follows: raun is injected 2 times, after injection accounts for the 20% of accumulated dose, 24 hours for the first time, second of injection
Account for the 80% of accumulated dose；Milter is injected 1 time, and injection time is synchronous with second of injection time of raun, second of note of dosage and raun
It is identical to penetrate dosage.
The step C before squeezing ovum to raun, first collects milter sperm and carries out microscopy, the sperm of microscopy qualification is at 4 DEG C
It is kept in dark place spare.
The selection of parent population of the present invention: selection is healthy and strong, physically well develops, the parent population without obvious wound and without deformity, selects
0.50~1.00 kg of raun specification, 0.52 kg of average out to；Milter 0.25~0.56kg of specification, 0.31 kg of average out to；It will
Selected point naked carp parent population uses the NaCl solution soaking disinfection of concentration 2~3% before hastening parturition, then separate by male and female and temporarily support.
The present invention hastens parturition in three batches.Preferable 11 tail of raun, 18 tail of milter are developed in selection for the first time；Second of selection
42 tail of raun, 50 tail of milter；Third time selection 39 tail of raun, milter 46.
Raun of wherein hastening parturition amounts to 92 tails, and the mean spawning rate reaches 91.96 ﹪, obtains fertilized eggs 1,200,000 altogether, averagely
Fertilization rate reached is to 62.87 ﹪.
The sharp naked carp spawning rate of table 1.
The sharp naked carp rate of fertilization of table 2.
A specific embodiment of the invention above described embodiment only expresses, the description thereof is more specific and detailed, but can not
Therefore limitations on the scope of the patent of the present invention are interpreted as.It should be pointed out that for those of ordinary skill in the art,
Without departing from the inventive concept of the premise, various modifications and improvements can be made, and these are all within the scope of protection of the present invention.
Claims (6)
1. a kind of artificial fecundation method of point naked carp, which comprises the following steps:
A, parent population selects: the April, and fishing developmental stage enters the wild sharp naked carp of IV phase as artificial propagation parent, to parent
After carrying out disinfection, separately female, milter is temporarily supported spare；
B, artificial induced spawning: luteotropin releasing hormone d-ala analog LRH-A is used2With carp pituitary PG mixture as ocyodinic,
LRH-A2Dosage be 10ug/kg, the dosage of PG mixture is 8mg/kg；Using biphasic injection, sharp naked carp raun is urged
It produces；
C, artificial insemination: being inseminated using anhydrous dry method, and raun passes through 48~72 hours Effect times, is manually squeezed to raun
Ovum is squeezed into sharp naked carp ovum grain in dry ceramic whiteware bowl, after pouring into the sperm of 3~4 drop milters, is sufficiently stirred 30 seconds, is added clear
Water stands 1 minute after gentle agitation；
D, hatch: the fertilized eggs after standing are washed 2~3 times with clear water repeatedly, the mucus in fertilized eggs are removed, then by unsticking
Fertilized eggs afterwards are laid in hatching frame, are hatched；Hatch bolting silk mesh size 1.0mm in frame, in hatching process, uses
Miniflow water is managed, and fertilized eggs is kept to remain static in hatching frame;The ovum not being fertilized is selected out with point on inspection
And dead embryo；Under conditions of 10~12 DEG C of water temperature, after hatching 10~13 days, i.e., rupture of membranes becomes juvenile fish.
2. the artificial fecundation method of sharp naked carp according to claim 1, which is characterized in that further include parent population postnatal care: adopting
Complete essence and the parent population after ovum is squeezed, first used glucose solution soaking 1 hour of 1%, then every fish injects gentamicin, injects
After parent population be transferred in running water technology pond, lure it to ingest using small-sized cold water fishes as bait fish.
3. the artificial fecundation method of sharp naked carp according to claim 2, which is characterized in that the dosage of the injection gentamicin
For 0.2mg/kg weight.
4. the artificial fecundation method of sharp naked carp according to claim 1, which is characterized in that the step A, with concentration 2~
3% NaCl solution, which impregnates parent population 30 minutes, to be sterilized.
5. the artificial fecundation method of sharp naked carp according to claim 1, which is characterized in that biphasic injection are as follows: raun injection 2
Secondary, after injection accounts for the 20% of accumulated dose, 24 hours for the first time, second of injection accounts for the 80% of accumulated dose；Milter is injected 1 time, injection
Time is synchronous with second of injection time of raun, and dosage is identical as second of injection dosage of raun.
6. the artificial fecundation method of sharp naked carp according to claim 1, which is characterized in that the step C squeezes ovum to raun
Before, it first collects milter sperm and carries out microscopy, the sperm of microscopy qualification is kept in dark place spare at 4 DEG C.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112293336A (en) * | 2020-12-07 | 2021-02-02 | 西藏自治区农牧科学院水产科学研究所 | Domestication system and method for adult wild cyprinus carpio |
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