CN106879510A - The induced spawning method of the naked row buttocks fish in Lhasa - Google Patents
The induced spawning method of the naked row buttocks fish in Lhasa Download PDFInfo
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- CN106879510A CN106879510A CN201710236310.7A CN201710236310A CN106879510A CN 106879510 A CN106879510 A CN 106879510A CN 201710236310 A CN201710236310 A CN 201710236310A CN 106879510 A CN106879510 A CN 106879510A
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- 241000251468 Actinopterygii Species 0.000 title claims abstract description 54
- 210000001217 buttock Anatomy 0.000 title claims abstract description 47
- 238000000034 method Methods 0.000 title claims abstract description 41
- 230000009027 insemination Effects 0.000 claims abstract description 10
- 238000011534 incubation Methods 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 54
- 238000002347 injection Methods 0.000 claims description 37
- 239000007924 injection Substances 0.000 claims description 37
- 230000012447 hatching Effects 0.000 claims description 27
- 102000002322 Egg Proteins Human genes 0.000 claims description 24
- 108010000912 Egg Proteins Proteins 0.000 claims description 24
- 210000004681 ovum Anatomy 0.000 claims description 24
- 239000011152 fibreglass Substances 0.000 claims description 22
- 239000003814 drug Substances 0.000 claims description 20
- 229940079593 drug Drugs 0.000 claims description 19
- 230000002196 ecbolic effect Effects 0.000 claims description 19
- 239000011521 glass Substances 0.000 claims description 15
- 230000009471 action Effects 0.000 claims description 12
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 12
- 210000003746 feather Anatomy 0.000 claims description 12
- 229910052760 oxygen Inorganic materials 0.000 claims description 12
- 239000001301 oxygen Substances 0.000 claims description 12
- 230000000638 stimulation Effects 0.000 claims description 12
- 239000000919 ceramic Substances 0.000 claims description 11
- 239000012286 potassium permanganate Substances 0.000 claims description 11
- 229910000831 Steel Inorganic materials 0.000 claims description 8
- 239000010959 steel Substances 0.000 claims description 8
- 239000002504 physiological saline solution Substances 0.000 claims description 7
- 210000000582 semen Anatomy 0.000 claims description 7
- 210000000006 pectoral fin Anatomy 0.000 claims description 6
- 239000003488 releasing hormone Substances 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- 241000252233 Cyprinus carpio Species 0.000 claims 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims 1
- 210000004556 brain Anatomy 0.000 claims 1
- 229910052700 potassium Inorganic materials 0.000 claims 1
- 239000011591 potassium Substances 0.000 claims 1
- 238000009360 aquaculture Methods 0.000 abstract description 3
- 244000144974 aquaculture Species 0.000 abstract description 3
- 238000011161 development Methods 0.000 abstract description 3
- 235000013601 eggs Nutrition 0.000 description 9
- 241000252210 Cyprinidae Species 0.000 description 7
- 230000000694 effects Effects 0.000 description 6
- 230000001817 pituitary effect Effects 0.000 description 5
- 238000013019 agitation Methods 0.000 description 4
- 238000009395 breeding Methods 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- 230000032696 parturition Effects 0.000 description 4
- 210000001015 abdomen Anatomy 0.000 description 3
- 241000252206 Cypriniformes Species 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000004720 fertilization Effects 0.000 description 2
- 241000065747 Schizopygopsis Species 0.000 description 1
- 241000065749 Schizopygopsis younghusbandi Species 0.000 description 1
- 241000685317 Triplophysa Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003673 groundwater Substances 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052573 porcelain Inorganic materials 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/003—Aquaria; Terraria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/60—Fish, e.g. seahorses; Fish eggs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/04—Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
- A61K38/08—Peptides having 5 to 11 amino acids
- A61K38/09—Luteinising hormone-releasing hormone [LHRH], i.e. Gonadotropin-releasing hormone [GnRH]; Related peptides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Veterinary Medicine (AREA)
- Biodiversity & Conservation Biology (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Medicinal Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Animal Husbandry (AREA)
- Pharmacology & Pharmacy (AREA)
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- Endocrinology (AREA)
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- Gastroenterology & Hepatology (AREA)
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- Proteomics, Peptides & Aminoacids (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention provides a kind of induced spawning method of the naked row buttocks fish in Lhasa, comprise the following steps：A, parent population selection；B, artificial induced spawning；C, artificial insemination；D, artificial incubation.Simply, workable, practical application is good for the method, it is possible to achieve the scale of the wild naked row buttocks fish in Lhasa and standardization artificial induced spawning, promotes the aquaculture development of the naked row buttocks fish in Lhasa, effectively protects and utilize the naked row buttocks wild resource in Lhasa.
Description
Technical field
The invention belongs to technical field of aquaculture, and in particular to a kind of induced spawning method of the naked row buttocks fish in Lhasa.
Background technology
Tibet possesses abundant water resource, is the water tower in the world.According to statistics, Tibet differs in size lake more than 2000,
Lake gross area km more than 240002, account for the 30% of the national lake gross area.On the vast plateau in Tibet, drainage area is more than
10000km2River have more than 20 bars, more than 2000km2River it is a plurality of 100, including Jinsha jiang River, the Lancang River, Nujiang, Ya Lu
The rivers such as Zang Bujiang, friend's song, Senge Zangbu, rise or flow through Tibet.The fish being distributed in numerous rivers and lake have three
Big monoid, is respectively schizothoracin class, Triplophysa and Select-Committee sections fish.Wherein Lhasa is naked splits buttocks (Schizopygopsis
Younghusbandi) it is under the jurisdiction of Cypriniformes (Cypriniformes), Cyprinidae (Cyprinidae), Schizothoracinae
(Schizothoracinae), naked row buttocks fish category (Schizopygopsis), is commonly called as native fish, is the important original inhabitants' warp of Tibet plateau
One of Ji fish, belong to cold water fishes, dry Yarlung Zangbo River middle and upper reaches, tributary and its attached water body are mainly distributed on, for me
State endemic species.The naked row buttocks raun in Lhasa is more than milter in nature, belongs to synchronous Type of Spawning in batches, lays eggs 1 time within 1 year, mainly
March~April is concentrated on, breeding period is shorter.In recent years, as Tibet's economy society is fast-developing, transportation network is more convenient,
Consumer market demand constantly increases, and artificially excessively captures phenomenon extremely serious；In addition, being established on Yarlung Zangbo River mainstream and tributary
Power station, hinders fish migration passage, forces the stock number of the naked row buttocks fish in Lhasa to tend to declining.
Patent of invention CN201410142435.X discloses that a kind of soft thorn is naked to split the artificial propagation of buttocks fish and hatch fish roe method,
Patent of invention CN201310449845.4 discloses a kind of Yellow River fish artificial fecundation method, urging in two kinds of induced spawning methods
Produce composition of medicine and dosage is not suitable for the artificial induced spawning of the naked row buttocks fish in Lhasa.
The content of the invention
In order to realize the full artificial breeding technology of Lhasa schizothoracin, the present invention has carried out the naked row buttocks artificial propagation techniques in Lhasa
A kind of research, there is provided induced spawning method suitable for the naked row buttocks fish in Lhasa, the method is simple, workable, and practice should
It is good with property, it is possible to achieve the scale of the wild naked row buttocks fish in Lhasa and standardization artificial induced spawning.
Technical scheme is as follows：
A kind of induced spawning method of the naked row buttocks fish in Lhasa, comprises the following steps：
A, parent population selection
Selection it is healthy and strong, physically well develop, without obvious wound and without deformity the naked row buttocks parent population in wild Lhasa, choose parent
Fish is carried out disinfection before pond is entered with potassium permanganate, is then divided in parent population storage pond, holding pond by male and female and temporarily supported 1 day；Storage pond, holding pond is used in advance
Potassium permanganate invades bubble one week, is cleaned up with clear water afterwards；The parent population storage pond, holding pond water temperature be 11~12 DEG C, dissolved oxygen be 6~
8mg/L, pH are 7.2~7.5.
By Yarlung Zangbo River fishery resources survey for many years, it is found that Lhasa Luo Liekao spawning grounds water temperature is 11 DEG C or so, it is molten
Oxygen be higher than 6mg/L, pH be 7.1~7.6 between, its water quality environment laid eggs under field conditions (factors) is simulated as far as possible.
Preferably, the proportioning of male and female parent population is 1:1.2, because the naked row buttocks fish raun in Lhasa is more than milter, institute in nature
It is as much as possible to make good use of milter with when artificial induced spawning is bred, can guarantee that female fish egg is preferably fertilized again, it is ensured that its
Scale hasten parturition artificial induced spawning breeding.
B, artificial induced spawning
Using pectoral fin base portion injection, injection dosage is：5 μ g luteotropin releasing hormone d-ala analogs (LRH-A2)/kg+
2.5mg carps pituitary (PG)/kg+1ml fish physiological saline；
The concentration of the fish physiological saline is 0.65 ﹪.
Raun is injected 2 times, for the first time injection 30%, interval second injection 70% after 24 hours；Milter is injected 1 time, with
Raun is injected for second and synchronously carries out, and injection dosage halves.Together be put into female milter after terminating by second oxytocic drug injection
In the spawning pond of fiberglass, parent population is observed every 4 hours, check that circulation runs well, while water pump is opened,
Carry out 20 minutes stimulation by running water；
Preferably, when injecting oxytocic drug, obstacle is wrapped up above syringe needle, exposed needle length is 0.5cm, in order to avoid in rule
Syringe needle insertion is too deep during modelling artificial induced spawning, injures the vitals such as parent population heart.
Preferably, the flow velocity of described stimulation by running water is 0.1-0.2m/s, identical with nature spawning ground water velocity.
C, artificial insemination
After second 24h of oxytocic drug injection, artificial induced spawning parent population is checked, inseminated using dry method；
Raun body surface moisture is dried with dry towel first, action is soft, prevents scratch, ovum is clamp-oned a diameter of
In the white ceramic bowl of 30cm, be extruded into milter seminal fluid on ovum by same action and method, is gently agitated for 10 seconds with feather, with wooden dipper along porcelain
Tub wall slowly adds a little clear water, has just flooded ovum preferably, is gently agitated for 20 seconds with feather again, adds clear water to wash afterwards three times；
D, artificial incubation
Embryonated egg is put into hatching inframe, micro- Lotic hatching is carried out, it is 11~12 DEG C to control water temperature, and dissolved oxygen is 6~8mg/
L, pH are 7.2~7.5.
Preferably, the hatching frame is that mesh is the wooden hatching frame of 10 purposes, is suitable to the hatching of fish-egg.
Preferably, the ovum density of putting of hatching inframe is 4.17 ten thousand Li/㎡.
Preferably, micro- Lotic hatching refers to that water-carrying capacity is 1.5 cubes ms/h, and hatching effect is preferable.
The parent population storage pond, holding pond of step A of the present invention is 1.2m fiberglass round tanks, and the spawning pond of step B is a diameter of
The fiberglass circle spawning pond of 2.5m.Fiberglass round tank surface is smooth first, and wild Lhasa schizothoracin has certain after being put into
Stress effect, prevent in response to swash and abrade fish body body surface, a diameter of 1.2m separately temporarily supports the naked row buttocks fish in Lhasa, be conducive to behaviour
Make, convenient management；The fiberglass circle spawning pond of 2.5m is selected after second pin, because male and female parent population now is placed on into one
Rise, in addition, spawning pond has boost pump, certain current can be carried out to parent population to stimulate.So in order to reach more preferable effect and side
Convenient to operate just selects the spawning pond of diameter 1.2m storage pond, holding ponds and diameter 2.5m.
The beneficial effects of the present invention are：
1st, the method is simple, and workable, practical application is good, it is possible to achieve the scale of the wild naked row buttocks fish in Lhasa
With standardization artificial induced spawning, promote the aquaculture development of the naked row buttocks fish in Lhasa, effectively protect and utilize the naked wild money of row buttocks in Lhasa
Source.The artificial the mean spawning rate of the naked row buttocks fish in Lhasa reaches 90.4 ﹪, average fertilization rate reached to 87 ﹪.
2nd, be divided among for male and female parent population temporarily to be supported 1 day in parent fish pond by the present invention, to adapt to artificial induced spawning environment, second injection
After, then female milter is put together, may advantageously facilitate parent population sperm and ovum reaches maturation.
3rd, the present invention have selected the specific oxytocic drug for being suitable to the naked row buttocks fish in Lhasa according to the characteristic of the naked row buttocks fish in Lhasa
Combination and dosage, 5 μ g luteotropin releasing hormone d-ala analogs/kg+2.5mg carps pituitary/kg+1ml fish physiological saline, obtain
To best spawning effect.
4th, the proportioning of male and female parent population is set to 1:1.2, be due in nature the naked row buttocks fish raun in Lhasa be more than milter,
So when artificial induced spawning is bred, it is as much as possible to make good use of milter, can guarantee that female fish egg is preferably fertilized again, it is ensured that
Its scale hasten parturition artificial induced spawning breeding.
5th, parent population storage pond, holding pond of the invention is 1.2m fiberglass round tanks, and spawning pond is that the fiberglass of a diameter of 2.5m is circular
Spawning pond.Fiberglass round tank surface is smooth first, wild Lhasa schizothoracin be put into after have certain stress effect, prevent because
Stress and abrade fish body body surface, a diameter of 1.2m separately temporarily supports the naked row buttocks fish in Lhasa, is conducive to operation, convenient management；Second
The fiberglass circle spawning pond of 2.5m is selected after pin, because now male and female parent population is put together, in addition, spawning pond has
Boost pump, certain current can be carried out to parent population to stimulate.So just selecting diameter to reach more preferable effect and convenient operation
The spawning pond of 1.2m storage pond, holding ponds and diameter 2.5m.
Specific embodiment
Essentiality content of the invention is described in further detail with reference to specific embodiment.
Embodiment 1
A kind of induced spawning method of the naked row buttocks fish in Lhasa, comprises the following steps：
A, parent population selection
Selection it is healthy and strong, physically well develop, without obvious wound and without deformity the naked row buttocks parent population in wild Lhasa, choose parent
Fish is carried out disinfection before pond is entered with potassium permanganate, is then divided in parent population storage pond, holding pond by male and female and temporarily supported 1 day；Storage pond, holding pond is used in advance
Potassium permanganate invades bubble one week, is cleaned up with clear water afterwards；The parent population storage pond, holding pond water temperature is 11 DEG C, and dissolved oxygen is 6mg/L, and pH is
7.2；
B, artificial induced spawning
Using pectoral fin base portion injection, injection dosage is：5 μ g luteotropin releasing hormone d-ala analogs/kg+2.5mg carps
Pituitary/kg+1ml fish physiological saline；
Raun is injected 2 times, for the first time injection 30%, interval second injection 70% after 24 hours；Milter is injected 1 time, with
Raun is injected for second and synchronously carries out, and injection dosage halves.Together be put into female milter after terminating by second oxytocic drug injection
In the spawning pond of fiberglass, parent population is observed every 4 hours, check that circulation runs well, while water pump is opened,
Carry out 20 minutes stimulation by running water；
C, artificial insemination
After second 24h of oxytocic drug injection, artificial induced spawning parent population is checked, inseminated using dry method；
D, artificial incubation
Embryonated egg is put into hatching inframe, micro- Lotic hatching is carried out, controls water temperature for 11 DEG C, dissolved oxygen is 6mg/L, and pH is
7.2。
Embodiment 2
A kind of induced spawning method of the naked row buttocks fish in Lhasa, comprises the following steps：
A, parent population selection
Selection it is healthy and strong, physically well develop, without obvious wound and without deformity the naked row buttocks parent population in wild Lhasa, choose parent
Fish is carried out disinfection before pond is entered with potassium permanganate, is then divided in parent population storage pond, holding pond by male and female and temporarily supported 1 day；Storage pond, holding pond is used in advance
Potassium permanganate invades bubble one week, is cleaned up with clear water afterwards；The parent population storage pond, holding pond water temperature is 12 DEG C, and dissolved oxygen is 8mg/L, and pH is
7.5；
B, artificial induced spawning
Using pectoral fin base portion injection, injection dosage is：5 μ g luteotropin releasing hormone d-ala analogs/kg+2.5mg carps
Pituitary/kg+1ml fish physiological saline；
Raun is injected 2 times, for the first time injection 30%, interval second injection 70% after 24 hours；Milter is injected 1 time, with
Raun is injected for second and synchronously carries out, and injection dosage halves.Together be put into female milter after terminating by second oxytocic drug injection
In the spawning pond of fiberglass, parent population is observed every 4 hours, check that circulation runs well, while water pump is opened,
Carry out 20 minutes stimulation by running water；
C, artificial insemination
After second 24h of oxytocic drug injection, artificial induced spawning parent population is checked, inseminated using dry method；
D, artificial incubation
Embryonated egg is put into hatching inframe, micro- Lotic hatching is carried out, controls water temperature for 12 DEG C, dissolved oxygen is 8mg/L, and pH is
7.5。
Embodiment 3
A kind of induced spawning method of the naked row buttocks fish in Lhasa, comprises the following steps：
A, parent population selection
Selection it is healthy and strong, physically well develop, without obvious wound and without deformity the naked row buttocks parent population in wild Lhasa, choose parent
Fish is carried out disinfection before pond is entered with potassium permanganate, is then divided in parent population storage pond, holding pond by male and female and temporarily supported 1 day；Storage pond, holding pond is used in advance
Potassium permanganate invades bubble one week, is cleaned up with clear water afterwards；The parent population storage pond, holding pond water temperature is 11.5 DEG C, and dissolved oxygen is 7mg/L, pH
It is 7.3；
B, artificial induced spawning
Using pectoral fin base portion injection, injection dosage is：5 μ g luteotropin releasing hormone d-ala analogs/kg+2.5mg carps
Pituitary/kg+1ml fish physiological saline；
Raun is injected 2 times, for the first time injection 30%, interval second injection 70% after 24 hours；Milter is injected 1 time, with
Raun is injected for second and synchronously carries out, and injection dosage halves.Together be put into female milter after terminating by second oxytocic drug injection
In the spawning pond of fiberglass, parent population is observed every 4 hours, check that circulation runs well, while water pump is opened,
Carry out 20 minutes stimulation by running water；
C, artificial insemination
After second 24h of oxytocic drug injection, artificial induced spawning parent population is checked, inseminated using dry method；
D, artificial incubation
Embryonated egg is put into hatching inframe, micro- Lotic hatching is carried out, controls water temperature for 11.5 DEG C, dissolved oxygen is 7mg/L, and pH is
7.3。
Embodiment 4
The present embodiment is on the basis of embodiment 1：
The proportioning of male and female parent population is 1:1.2.
Embodiment 5
The present embodiment is on the basis of embodiment 1：
The proportioning of male and female parent population is 1:1.2.
The parent population storage pond, holding pond of the step A is 1.2m fiberglass round tanks, and the spawning pond of step B is the glass of a diameter of 2.5m
Glass steel circle spawning pond.
Embodiment 6
The present embodiment is on the basis of embodiment 1：
The proportioning of male and female parent population is 1:1.2.
The parent population storage pond, holding pond of the step A is 1.2m fiberglass round tanks, and the spawning pond of step B is the glass of a diameter of 2.5m
Glass steel circle spawning pond.
The step B wraps up obstacle when oxytocic drug is injected above syringe needle, exposed needle length is 0.5cm.
Embodiment 7
The present embodiment is on the basis of embodiment 1：
The proportioning of male and female parent population is 1:1.2.
The parent population storage pond, holding pond of the step A is 1.2m fiberglass round tanks, and the spawning pond of step B is the glass of a diameter of 2.5m
Glass steel circle spawning pond.
The step B wraps up obstacle when oxytocic drug is injected above syringe needle, exposed needle length is 0.5cm.
Described step B, the flow velocity of stimulation by running water is 0.1m/s.
Embodiment 8
The present embodiment is on the basis of embodiment 1：
The proportioning of male and female parent population is 1:1.2.
The parent population storage pond, holding pond of the step A is 1.2m fiberglass round tanks, and the spawning pond of step B is the glass of a diameter of 2.5m
Glass steel circle spawning pond.
The step B wraps up obstacle when oxytocic drug is injected above syringe needle, exposed needle length is 0.5cm.
Described step B, the flow velocity of stimulation by running water is 0.2m/s.
The dry method insemination refers to dry raun body surface moisture with dry towel first, and action is soft, prevents from abrading skin
Skin, ovum is clamp-oned in the white ceramic bowl of a diameter of 30cm, and be extruded into milter seminal fluid on ovum by same action and method, with feather gently
Agitation 10 seconds, a little clear water is slowly added with wooden dipper along ceramic bowl wall, has just flooded ovum preferably, is gently agitated for 20 seconds with feather again, afterwards
Plus clear water is washed three times.
Embodiment 9
The present embodiment is on the basis of embodiment 1：
The proportioning of male and female parent population is 1:1.2.
The parent population storage pond, holding pond of the step A is 1.2m fiberglass round tanks, and the spawning pond of step B is the glass of a diameter of 2.5m
Glass steel circle spawning pond.
The step B wraps up obstacle when oxytocic drug is injected above syringe needle, exposed needle length is 0.5cm.
Described step B, the flow velocity of stimulation by running water is 0.15m/s.
The dry method insemination refers to dry raun body surface moisture with dry towel first, and action is soft, prevents from abrading skin
Skin, ovum is clamp-oned in the white ceramic bowl of a diameter of 30cm, and be extruded into milter seminal fluid on ovum by same action and method, with feather gently
Agitation 10 seconds, a little clear water is slowly added with wooden dipper along ceramic bowl wall, has just flooded ovum preferably, is gently agitated for 20 seconds with feather again, afterwards
Plus clear water is washed three times.
Described D steps, the ovum density of putting of hatching inframe is 4.1 ten thousand Li/㎡.
Embodiment 10
The present embodiment is on the basis of embodiment 1：
The proportioning of male and female parent population is 1:1.2.
The parent population storage pond, holding pond of the step A is 1.2m fiberglass round tanks, and the spawning pond of step B is the glass of a diameter of 2.5m
Glass steel circle spawning pond.
The step B wraps up obstacle when oxytocic drug is injected above syringe needle, exposed needle length is 0.5cm.
Described step B, the flow velocity of stimulation by running water is 0.16m/s.
The dry method insemination refers to dry raun body surface moisture with dry towel first, and action is soft, prevents from abrading skin
Skin, ovum is clamp-oned in the white ceramic bowl of a diameter of 30cm, and be extruded into milter seminal fluid on ovum by same action and method, with feather gently
Agitation 10 seconds, a little clear water is slowly added with wooden dipper along ceramic bowl wall, has just flooded ovum preferably, is gently agitated for 20 seconds with feather again, afterwards
Plus clear water is washed three times.
Described D steps, the ovum density of putting of hatching inframe is 4.2 ten thousand Li/㎡.
Micro- Lotic hatching of the D steps refers to that water-carrying capacity is 1.5 cubes ms/h.
Embodiment 11
The present embodiment is on the basis of embodiment 1：
The proportioning of male and female parent population is 1:1.2.
The parent population storage pond, holding pond of the step A is 1.2m fiberglass round tanks, and the spawning pond of step B is the glass of a diameter of 2.5m
Glass steel circle spawning pond.
The step B wraps up obstacle when oxytocic drug is injected above syringe needle, exposed needle length is 0.5cm.
Described step B, the flow velocity of stimulation by running water is 0.12m/s.
The dry method insemination refers to dry raun body surface moisture with dry towel first, and action is soft, prevents from abrading skin
Skin, ovum is clamp-oned in the white ceramic bowl of a diameter of 30cm, and be extruded into milter seminal fluid on ovum by same action and method, with feather gently
Agitation 10 seconds, a little clear water is slowly added with wooden dipper along ceramic bowl wall, has just flooded ovum preferably, is gently agitated for 20 seconds with feather again, afterwards
Plus clear water is washed three times.
Described D steps, the ovum density of putting of hatching inframe is 4.17 ten thousand Li/㎡.
Micro- Lotic hatching of the D steps refers to that water-carrying capacity is 1.5 cubes ms/h.
The hatching frame of the D steps is that mesh is the wooden hatching frame of 10 purposes.
The selection of parent population of the present invention
Selection it is healthy and strong, physically well develop, without obvious wound and without deformity parent population, select 0.25~0.65kg of specification,
Average out to 0.42kg；The tail of milter 230,0.25~0.56kg of specification, average out to 0.31kg, male and female proportioning are 1:1.2；To choose
The naked row buttocks parent population in wild Lhasa enter pond before carried out disinfection with potassium permanganate, concentration for 20g/m3 invade bubble 10 minutes, then
Separately temporarily supported in a diameter of 1.2m fiberglass round tank by male and female, storage pond, holding pond invades bubble one with potassium permanganate (20g/m3) in advance
In week, cleaned up with clear water afterwards, parent population storage pond, holding pond water temperature is 11~12 DEG C, dissolved oxygen is 6~8mg/L, pH is 7.2~7.5.
Parent population Sex judging of the present invention
Raun:Raun individuality is relatively large, and hand touches that body is smooth, and belly substantially expands, soft, flexible, gonopore is bright
Aobvious protrusion is mellow and full, anal fin least significant end fin ray not bifurcated.
Milter：Milter individuality is relatively small, and belly is flat, and it is coarse that hand touches body, and star-pursuing is furnished with pectoral fin to tail fin trunk,
Anal fin fin ray is flourishing, and with obvious zigzag, gently extruding belly has milky seminal fluid to flow out, and can quickly be dissipated in water
Open.
On April 2nd, 2016, April 8, April 12, hastened parturition in three batches.The preferable raun 51 of selection development for the first time
Tail, the tail of milter 68；Second selection tail of raun 82, the tail of milter 90；The third time selection tail of raun 59, milter 72.
Raun of wherein hastening parturition amounts to 174 tails, and the mean spawning rate reaches 90.4 ﹪, and 700,000, embryonated egg is obtained altogether, averagely receives
Smart rate reaches 87 ﹪.
The naked row buttocks spawning rate in the Lhasa of table 1.
The naked row buttocks rate of fertilization in the Lhasa of table 2.
Embodiment described above only expresses specific embodiment of the invention, and its description is more specific and detailed, but simultaneously
Therefore the limitation to the scope of the claims of the present invention can not be interpreted as.It should be pointed out that for one of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to guarantor of the invention
Shield scope.
Claims (9)
1. a kind of induced spawning method of the naked row buttocks fish in Lhasa, comprises the following steps：
A, parent population selection
Selection it is healthy and strong, physically well develop, without obvious wound and without deformity the naked row buttocks parent population in wild Lhasa, choose parent population and exist
Carried out disinfection with potassium permanganate before entering pond, be then divided in parent population storage pond, holding pond by male and female and temporarily supported 1 day；Storage pond, holding pond uses Gao Meng in advance
Sour potassium invades bubble one week, is cleaned up with clear water afterwards；The parent population storage pond, holding pond water temperature is 11~12 DEG C, and dissolved oxygen is 6~8mg/L,
PH is 7.2~7.5；
B, artificial induced spawning
Using pectoral fin base portion injection, injection dosage is：5 μ g luteotropin releasing hormone d-ala analogs/kg+2.5mg carp brains hang down
Body/kg+1ml fish physiological saline；
Raun is injected 2 times, for the first time injection 30%, interval second injection 70% after 24 hours；Milter is injected 1 time, with raun
Injecting for second is synchronously carried out, and injection dosage halves.Female milter is put into glass by second oxytocic drug injection together after terminating
In the spawning pond of steel, parent population is observed every 4 hours, check that circulation runs well, while opening water pump, carry out
20 minutes stimulation by running water；
C, artificial insemination
After second 24h of oxytocic drug injection, artificial induced spawning parent population is checked, inseminated using dry method；
D, artificial incubation
Embryonated egg is put into hatching inframe, micro- Lotic hatching is carried out, it is 11~12 DEG C to control water temperature, and dissolved oxygen is 6~8mg/L, pH
It is 7.2~7.5.
2. the induced spawning method of the naked row buttocks fish in Lhasa according to claim 1, it is characterised in that：The proportioning of male and female parent population is
1:1.2。
3. the induced spawning method of the naked row buttocks fish in Lhasa according to claim 1, it is characterised in that：The parent population of the step A
Storage pond, holding pond is 1.2m fiberglass round tanks, and the spawning pond of step B is the fiberglass circle spawning pond of a diameter of 2.5m.
4. the induced spawning method of the naked row buttocks fish in Lhasa according to claim 1, it is characterised in that：The step B is in injection
During oxytocic drug, obstacle is wrapped up above syringe needle, exposed needle length is 0.5cm.
5. the induced spawning method of the naked row buttocks fish in Lhasa according to claim 1, it is characterised in that：Described step B, flowing water
The flow velocity of stimulation is 0.1-0.2m/s.
6. the induced spawning method of the naked row buttocks fish in Lhasa according to claim 1, it is characterised in that：The dry method is inseminated
Refer to, dry raun body surface moisture with dry towel first, action is soft, prevents scratch, and ovum is clamp-oned into a diameter of 30cm's
In white ceramic bowl, be extruded into milter seminal fluid on ovum by same action and method, is gently agitated for 10 seconds with feather, slow along ceramic bowl wall with wooden dipper
Slow plus a little clear water, has just flooded ovum preferably, is gently agitated for 20 seconds with feather again, adds clear water to wash afterwards three times.
7. the induced spawning method of the naked row buttocks fish in Lhasa according to claim 1, it is characterised in that：Described D steps, hatching
The ovum density of putting of inframe is the Li/㎡ of 4.1-4.2 ten thousand.
8. the induced spawning method of the naked row buttocks fish in Lhasa according to claim 1, it is characterised in that：The miniflow of the D steps
Water hatching refers to that water-carrying capacity is 1.5 cubes ms/h.
9. the induced spawning method of the naked row buttocks fish in Lhasa according to claim 1, it is characterised in that：The hatching of the D steps
Frame is that mesh is the wooden hatching frame of 10 purposes.
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| CN110178767A (en) * | 2019-07-12 | 2019-08-30 | 西藏自治区农牧科学院水产科学研究所 | A kind of artificial fecundation method of point naked carp |
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| CN104255615A (en) * | 2014-10-13 | 2015-01-07 | 丽江滇江鱼类繁育有限公司 | Artificial propagation method of schizopygopsis malacanthus |
| CN106035167A (en) * | 2016-06-24 | 2016-10-26 | 西藏自治区农牧科学院 | Wild schizopygopsis younghusbandi domesticating method |
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| CN103461253A (en) * | 2013-09-29 | 2013-12-25 | 四川省农业科学院水产研究所 | Artificial breeding method for Triplophysa siluroides |
| CN103875572A (en) * | 2014-04-10 | 2014-06-25 | 中国科学院昆明动物研究所 | Artificial propagation and roe incubation method for schizopygopsis malacanthus |
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| CN110178767A (en) * | 2019-07-12 | 2019-08-30 | 西藏自治区农牧科学院水产科学研究所 | A kind of artificial fecundation method of point naked carp |
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