CN102007875A - Leiocassis longirostris intensive artificial propagation method - Google Patents

Leiocassis longirostris intensive artificial propagation method Download PDF

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CN102007875A
CN102007875A CN2009100442839A CN200910044283A CN102007875A CN 102007875 A CN102007875 A CN 102007875A CN 2009100442839 A CN2009100442839 A CN 2009100442839A CN 200910044283 A CN200910044283 A CN 200910044283A CN 102007875 A CN102007875 A CN 102007875A
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water
cave
hatching
long
milter
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杨述奇
徐德平
贾建成
李文
杨术平
刘勇
万铁海
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王国平
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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Abstract

A Leiocassis longirostris intensive artificial propagation method, comprises parent fish selection, induced spawning, hatching. According to the present invention, individuals of Leiocassis longirostris whose bodies weigh more than 2.5 kilogram and body surfaces are smooth as parents, and the Leiocassis longirostris parents are disease-free and injurt-free, healthy, and appear sexually mature are selected. The ratio of female fishes and male fishes is 1:1 to 1:1.5. The induced spawning includes the installation of a specific oviposition pool and an oviposition-nest, preparation of oxytocic in different doses, and spawn taking. The hatching includes the determination of a hatching groove and the hatching condition. The method of the present invention saves manpower, material resources and financial resources, and can produce fry by bulk according to a plan in order to satisfy the requirements for fry of different customers, makes production and development be unified; and avoids severe destruction of nature ecology caused by killing the male parents in a large number.

Description

Long-snout catfish large-scale artificial propagation method
Technical field
The present invention relates to the freshwater fish culturing method, further be meant long-snout catfish large-scale artificial propagation method.
Background technology
Long-snout catfish belongs to Silurformes, Chang Ke, Catfish genus on taxonomy, gain the name than other fish are long because of its kiss, is commonly called as " river group ", " fertile a small bay in a river ", " white mute ".Long-snout catfish mainly is distributed in the Yangtze river basin and each downstream, big tributary, and Dongting Lake is one of main distributed areas.Long-snout catfish contains the meat rate up to more than 70%, has 13 kinds to be higher than grass carp in 18 seed amino acids, and especially glutamic acid and aspartic acid content are respectively up to 12.05% and 8.51%.Therefore, the long-snout catfish fine and tender taste, the meat flavour deliciousness is a kind of all-ages nutrient excellent product.The good reputation that " self-sufficient and strategically located region first fish " more arranged in Sichuan.Particularly Pekinese " steamed river group " and " river group raw fish " and with " fish maw " that its fish glue is made are treasure delicacies both domestic and external.Obtained unanimously praising of world's cuisines circle.
Long-snout catfish belongs to temperate water fishes, and its existence temperature is 1 ℃-38 ℃.Be adapted to big extremely waters and culture, but because protection of resources is in recent decades taken effective measures, cruel fishing is excessively captured and added environmental pollution and excessively slaughter milter, and natural production is fallen sharply.The fisherman is difficult to catch long-snout catfish now.The artificial induced spawning of the most employing of long-snout catfish artificial fecundation method at present kills hero and gets essence and carry out artificial insemination.The tens million of tails of male parent population of slaughtering the rivers stalwartness every year cause direct economic loss to cross hundred million yuan.Natural waters female-male proportion is seriously lacked of proper care, and the natural resources amount falls sharply, and makes this kind be endangered breed, has caused the common concern of aquatic products circle.Therefore, improve artificial fecundation method, the large-scale production fry and fingerling realizes that artificial releasing and protection of resources just can make this kind form dominant population, and then carries out industrialization development that its meaning is very far-reaching.
Summary of the invention
The technical problem to be solved in the present invention is, deficiency at prior art exists proposes a kind of long-snout catfish large-scale artificial propagation method, and this method can be saved human and material resources and financial resources, can in a planned way produce fry in batches to satisfy the fry demand of different user, make to produce and unify mutually with exploitation; Also can avoid causing heavy damage to natural, ecological because of killing the male parent in a large number.
Technical scheme of the present invention is that described long-snout catfish large-scale artificial propagation method is:
(1) parent population is selected: selective body focuses on more than the 2.5kg, and body surface smooth, anosis nothing wound, physique stalwartness, the sexually matured individuality of profile are as the parent, and (bar number) proportioning of raun and milter is a raun: milter=1: 1-1.5; The only long 0.4cm-0.6cm of the sexually matured raun gonapophysis of profile, gonopore is wide and justify, and color and luster is ruddy, and belly expands, and hand catches fish and makes the visible significantly ovary outline of belly up, and the hand belly is spongy; The sexually matured milter gonapophysis of profile is long and sharp, long 0.9cm-1.1cm;
Selecting sexually matured parent for use is the key of artificial propagation, and this piscinity maturation age was generally for 4 ages;
(2) hasten parturition:
A. the construction of spawning pond: the bionical attitude construction cave of laying eggs in general spawning pond, at every 100m 2Spawning pond in the 30-50 cave of laying eggs is set, the sparge pipe of diameter 10cm-20cm and pipe end sealing is set up in each bottom, cave, the hole for water spraying of one aperture 0.2cm-0.4cm is set every 10cm on the tube wall of sparge pipe, and pipe is outer relatively in the sparge pipe keeps positive hydraulic pressure to increase the cave dissolved oxygen from the outside injection water of hole for water spraying; Keep the spawning pond depth of water to be not less than 1.2 meters when laying eggs, making spawning pond keep flow velocity is that the miniflow water of 0.08 meter per second-0.12 meter per second is laid eggs to stimulate parent population;
The cave of laying eggs can be built with conventional building materials (brick, cement, tile etc.), and the hole diameter in each cave of laying eggs is 48cm-52cm, hole degree of depth 90cm-110cm, and cave inside diameter 70-80cm, the cave inwall is smooth, and the hole is towards differing;
Available iron wire is bundled into size and shape and the corresponding wire netting of described cave inner chamber, is attached on the wire netting as laying nest with 30-50 order PEF net, and each hangs on sign board and numbering with line each laying nest, and a laying nest is inserted in each cave of laying eggs;
B. medicine is hastened parturition: oxytocic drug is selected carp brain pendant (PG) and luteotropin releasing hormone d-ala analog (LRH-A for use 2), drug use dosage is per kilogram fish body weight medication LRH-A 230-40 μ g and PG1-1.5 are individual, i.e. LRH-A 230-40 μ g+PG1-1.5)/kg;
Used oxytocic drug is taked double injection:
For the first time: 25% of raun injectable drug accumulated dose, milter is not injected;
For the second time: raun has been annotated remaining dosage, and the milter injected dose is half of described medicine accumulated dose; Every tail parent population liquid medicine injection is 2ml;
The double injection time was separated by 12 hours, and the raun and the milter of finishing described oxytocic drug injection are put into spawning pond; The effect time is about 20 hours, 23-25 ℃ of the best of the water temperature of hastening parturition;
C. get ovum: determine the parent population situation of laying eggs by the laying nest marker number, change nest twice to get ovum, in order to avoid overlong time and parent population food ovum;
(3) hatching: laying nest is taken out, in clear water, wash to the greatest extent, put into the incubation tank hatching, to hatching fry; Incubation tank is generally the cement pit of long 4m-6m, wide 1.5m-2.5m, (colloid) water pipe of diameter 10cm-20cm is installed at the dual-side of groove, on water pipe, hole for water spraying is set every 5cm-10cm, and the water spraying direction that makes hole for water spraying becomes 40 ° of-50 ° of angles with the groove horizontal plane, implement water spray, water spray head (peak) and cistern horizontal plane drop 2.5m-3.5m; The hatching water requires water quality pure and fresh pollution-free, dissolved oxygen amount 6-7mg/L,
The incubation tank water inlet is provided with 40 orders-50 order nylon cloth screen pack filtered water inlet, and the delivery port of incubation tank groove is done fence with 60 order nylon cloths and run away to prevent fry;
Checked fish-egg and current in the hatching process every 30 minutes; As find that bad ovum, dead ovum in time remove.
Above-mentioned long-snout catfish large-scale artificial propagation method of the present invention; it is the integrated aquatic products work practical experience of decades of being engaged in of applicant; on basis by deep theoretical research and analysis; just establish through a large amount of and test repeatedly over a long time; it is the great achievement that fish genetic breeding aspect obtains; at nearly 1.5 hundred million tails of test site successful reproduction fry, spawning rate, spawning rate, incubation rate all maintain the leading position at home.
The present invention adopts the propagation technique of artificial induced spawning, natural spawning, promotes the back and can kill the male parent less more than 1,000 ten thousand kilograms every year, except that having reduced the direct economic loss that causes thus, has also avoided the destruction to natural, ecological; Breed fry with the inventive method, can satisfy the fry demand of different user; Also can work out long-term artificial releasing's plan in real time, pass through the artificial releasing, in 10 years, make the natural output of Dongting Lake and Yangtze river basin long-snout catfish return to the level fifties, according to the market demand it is carried out industrialization development, make long-snout catfish " fish maw ", " fishbone ", " shark's lip " etc. form brand, make " the fertile a small bay in a river in Dongting Lake " walk out the Hunan, go to the world.
As known from the above, the present invention is a kind of long-snout catfish large-scale artificial propagation method, and it can save human and material resources and financial resources, can in a planned way produce fry in batches to satisfy the fry demand of different user, makes to produce with exploitation to unify mutually; Also can avoid causing heavy damage to natural, ecological because of killing the male parent in a large number.
Embodiment
Embodiment 1: a kind of Wen Catfish large-scale artificial propagation method comprises:
(1) parent population is selected: selective body focuses on more than the 2.5kg, body surface smooth, anosis nothing wound, physique stalwartness, the sexually matured individuality of profile are as the parent, the proportioning of raun and milter is a raun: milter=1: 1.2, about the only long 0.5m of the sexually matured raun gonapophysis of profile, the hole is wide and justify, and color and luster is ruddy, and belly expands, hand catches fish and makes the visible significantly ovary outline of belly up, and the hand belly is spongy; The sexually matured milter gonapophysis of profile is long and sharp, is about 1cm;
(2) hasten parturition:
A. the construction of spawning pond: the bionical attitude construction cave of laying eggs in general spawning pond, at 100m 2Spawning pond in the 30-50 cave of laying eggs is set, build with conventional building materials (brick, cement, tile etc.) in the cave of laying eggs, the about 50m of hole diameter in each cave of laying eggs, the about 100cm of the hole degree of depth, cave inside diameter 70-80cm, the cave inwall is smooth, the hole is towards differing, the sparge pipe of diameter 15cm5 and pipe end sealing is set up in each bottom, cave, and the hole for water spraying of an aperture 0.3cm is set every 10cm on the tube wall of sparge pipe, increases the cave dissolved oxygen from the outside injection water of hole for water spraying; Keep 1.2 meters of the spawning pond depth of waters when laying eggs, making spawning pond keep flow velocity is that the miniflow water of 0.1 meter per second is laid eggs to stimulate parent population;
Be bundled into size and shape and the corresponding wire netting of described cave inner chamber (be the size in the size of wire netting and shape and cave and shape is close and can put into the cave) with iron wire, be attached on the wire netting as laying nest with 40 order PEF nets, each hangs on sign board and numbering with line each laying nest, and a laying nest is inserted in each cave of laying eggs;
B. medicine is hastened parturition: oxytocic drug is selected carp brain pendant (PG) and luteotropin releasing hormone d-ala analog (LRH-A for use 2), drug use dosage is per kilogram fish body weight medication LRH-A 235 μ g and PG1.2 are individual, i.e. LRH-A 235 μ g+PG1-1.2)/kg;
Used oxytocic drug is taked double injection:
For the first time: 25% of raun injectable drug accumulated dose, milter is not injected;
For the second time: raun has been annotated remaining dosage, and the milter injected dose is half of described medicine accumulated dose; Every tail parent population liquid medicine injection is 2ml;
The double injection time was separated by 12 hours, and the raun and the milter of finishing described oxytocic drug injection are put into spawning pond; The effect time is about 20 hours, 23-25 ℃ of the best of the water temperature of hastening parturition;
C. get ovum: determine the parent population situation of laying eggs by the laying nest marker number, change nest twice to get ovum, in order to avoid overlong time and parent population food ovum;
(3) hatching: laying nest is taken out, in clear water, wash to the greatest extent, put into the incubation tank hatching, to hatching fry; Incubation tank is generally the cement pit of long 5m, wide 2m, at the dual-side of groove the colloid water pipe of diameter 15cm is installed, and every 5cm-10cm hole for water spraying is set on water pipe, and makes water spraying direction and the groove horizontal plane angle at 45 of hole for water spraying, implements to spray water; The hatching water requires water quality pure and fresh pollution-free, dissolved oxygen amount 6-7mg/L, water spray head and cistern horizontal plane drop 3m;
The incubation tank water inlet is provided with 40 orders-50 order nylon cloth screen pack filtered water inlet, and the delivery port of incubation tank groove is done fence with 60 order nylon cloths and run away to prevent fry;
Checked fish-egg and current in the hatching process every 30 minutes; As find that bad ovum, dead ovum in time remove; Ban use of various kinds of drug to soak fish-egg, in order to avoid reduce the hatch fish roe rate or fry is caused medicament residue.
Embodiment 2: the oxytocic drug using dosage is per kilogram fish body weight medication LRH-A 230 μ g and PG1; All the other are with embodiment 1.
Embodiment 3: drug use dosage is per kilogram fish body weight medication LRH-A 240 μ g and PG1.5; All the other are with embodiment 1.

Claims (5)

1. long-snout catfish large-scale artificial propagation method is characterized in that this method is:
(1) parent population is selected: selective body focuses on more than the 2.5kg, and body surface smooth, anosis nothing wound, physique stalwartness, the sexually matured individuality of profile are as the parent; The proportioning of raun and milter is raun: milter=1: 1-1.5;
(2) hasten parturition:
A. the construction of spawning pond: the bionical attitude construction cave of laying eggs in general spawning pond, at every 100m 2Spawning pond in the 30-50 cave of laying eggs is set, the sparge pipe of diameter 10cm-20cm and pipe end sealing is set up in each bottom, cave, the hole for water spraying of one aperture 0.2cm-0.4cm is set every 10cm on the tube wall of sparge pipe, and pipe is outer relatively in the sparge pipe keeps positive hydraulic pressure to increase the cave dissolved oxygen from the outside injection water of hole for water spraying; Keep the spawning pond depth of water to be not less than 1.2 meters when laying eggs, making spawning pond keep flow velocity is that the miniflow water of 0.08 meter per second-0.12 meter per second is laid eggs to stimulate parent population; A laying nest is inserted in each cave of laying eggs;
B. medicine is hastened parturition: oxytocic drug is selected carp brain pendant PG and luteotropin releasing hormone d-ala analog LRH-A for use 2, drug use dosage is per kilogram fish body weight medication LRH-A 230-40 μ g and PG1-1.5;
Used oxytocic drug is taked double injection:
For the first time: 25% of raun injectable drug accumulated dose, milter is not injected;
For the second time: raun has been annotated remaining dosage, and the milter injected dose is half of described medicine accumulated dose; Every tail parent population liquid medicine injection is 2ml;
The double injection time was separated by 12 hours, and the raun and the milter of finishing described oxytocic drug injection are put into spawning pond; The effect time is 20 hours, 23-25 ℃ of the water temperature of hastening parturition;
C. get ovum: determine the parent population situation of laying eggs by the laying nest marker number, change nest and get ovum for twice;
(3) hatching: laying nest is taken out, in clear water, wash to the greatest extent, put into the incubation tank hatching, to hatching fry; Incubation tank is generally the cement pit of long 4m-6m, wide 1.5m-2.5m, (colloid) water pipe of diameter 10cm-20cm is installed at the dual-side of groove, on water pipe, hole for water spraying is set, and makes the water spraying direction of hole for water spraying become 40 ° of-50 ° of angles with the groove horizontal plane, implement water spray every 5cm-10cm; The hatching water requires water quality pure and fresh pollution-free, dissolved oxygen amount 6-7mg/L, water spray head and cistern horizontal plane drop 2.5m-3.5m;
The incubation tank water inlet is provided with 40 orders-50 order nylon cloth screen pack filtered water inlet, and the delivery port of incubation tank groove is done fence with 60 order nylon cloths.
2. according to the described long-snout catfish large-scale artificial of claim 1 propagation method, it is characterized in that the only long 0.4cm-0.6cm of the sexually matured raun gonapophysis of profile, gonopore is wide and justify, and color and luster is ruddy, and belly expands, hand catches fish and makes the visible significantly ovary outline of belly up, and the hand belly is spongy; The sexually matured milter gonapophysis of profile is long and sharp, long 0.9cm-1.1cm.
3. according to the described long-snout catfish large-scale artificial of claim 1 propagation method, it is characterized in that the hole diameter in each cave of laying eggs is 48cm-52cm, hole degree of depth 90cm-110cm, cave inside diameter 70-80cm, the cave inwall is smooth, and the hole is towards differing.
4. according to the described long-snout catfish large-scale artificial of claim 1 propagation method; it is characterized in that; be bundled into size and shape and the corresponding wire netting of described cave inner chamber with iron wire, be attached on the wire netting as laying nest with 30-50 order PEF net, each hangs on sign board and numbering with line each laying nest.
5. according to the described long-snout catfish large-scale artificial of claim 1 propagation method, it is characterized in that, in the hatching process, checked the flow velocity of fish-egg and water every 30 minutes; In time remove bad ovum, dead ovum.
CN2009100442839A 2009-09-07 2009-09-07 Leiocassis longirostris intensive artificial propagation method Pending CN102007875A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103651193A (en) * 2013-11-11 2014-03-26 青岛佰众化工技术有限公司 Artificial induced spawning fertilization method of leiocassis longirostris
CN104938385A (en) * 2015-06-23 2015-09-30 周天赐 Hemibarbus maculatus bleeker artificial reproduction method
CN105900879A (en) * 2016-04-25 2016-08-31 石家庄市环境监测中心 Efficient oryzias latipe breeding and hatching method
CN112335582A (en) * 2020-11-05 2021-02-09 安徽省农业科学院水产研究所 Artificial breeding method for hybrid pelteobagrus fulvidraco
CN115669579A (en) * 2022-12-05 2023-02-03 安徽省农业科学院水产研究所 Leiocassis longirostris parent breeding method

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103651193A (en) * 2013-11-11 2014-03-26 青岛佰众化工技术有限公司 Artificial induced spawning fertilization method of leiocassis longirostris
CN104938385A (en) * 2015-06-23 2015-09-30 周天赐 Hemibarbus maculatus bleeker artificial reproduction method
CN105900879A (en) * 2016-04-25 2016-08-31 石家庄市环境监测中心 Efficient oryzias latipe breeding and hatching method
CN105900879B (en) * 2016-04-25 2018-08-21 石家庄市环境监测中心 A kind of medaka efficiently breeding and hatching method
CN112335582A (en) * 2020-11-05 2021-02-09 安徽省农业科学院水产研究所 Artificial breeding method for hybrid pelteobagrus fulvidraco
CN115669579A (en) * 2022-12-05 2023-02-03 安徽省农业科学院水产研究所 Leiocassis longirostris parent breeding method

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Application publication date: 20110413