CN102742530B - Method for nature spawning and fertilization of scatophagus argus by artificial induction - Google Patents
Method for nature spawning and fertilization of scatophagus argus by artificial induction Download PDFInfo
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- CN102742530B CN102742530B CN201210254368.1A CN201210254368A CN102742530B CN 102742530 B CN102742530 B CN 102742530B CN 201210254368 A CN201210254368 A CN 201210254368A CN 102742530 B CN102742530 B CN 102742530B
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Abstract
The invention discloses a method for nature spawning and fertilization of scatophagus argus by artificial induction. According to the method, for parent fish, the gonad development and the maturation can be promoted by nutrition optimization, adjustment of the breeding condition and the combination with exogenous hormone in a net cage in a natural sea area, and the natural spawning and fertilization can be realized by artificial induction at artificial culture areas such as a spawning net cage or a cement breeding pool of a culture sea area. The effective rate of maturation acceleration of the parent fish is 89.1-100%, the spawning rate is 79.2-94.0%, the maturing rate of the milter is 100% and the total spawning amount of the parent fish is 48 million. The method disclosed by the invention has the advantages that the simpleness and convenience are achieved, convenience is brought for operation control, the technical cost is low, the effect is obvious and stable, the mature time and the spawning time of the parent fish can be more accurately judged according to the gonad development state and the ecological condition of the sea area, and the fertilized egg and the biological study materials are provided for breeding and production of the scatophagus argus fries.
Description
Technical field:
The invention belongs to aquaculture field, be specifically related to a kind of method of Scatophagus argus (Linnaeus) artificial induction natural spawning and fertilization.
Background technology:
Scatophagus argus (Linnaeus) is subordinate to Perciformes (Perciformes), money Gyrinocheilidae (Scatophagidae), Scatophagus argus (Linnaeus) belongs to (Scatophagus), for the torrid zone-subtropics wide temperature eurysalinity kind, be distributed in waters, India-Pacific Ocean, from Kuwait to Vanuatu island country, all there is distribution in South Japan to the vast waters such as butcher bird, the close western Ni Yaqundao in Crow.Scatophagus argus (Linnaeus) is that current China has unique species in these section fish of record, is mainly distributed in the South Sea, the Taiwan Straits and East Sea offshore cay and weed-filled seawater or the degree of saltiness water waters that crosses.
Scatophagus argus (Linnaeus) is a kind of famous and precious marine fish with very high ornamental value and edibility.Grow fast, disease-resistant strong stress resistance, not only can normal growth and growth in seawater and degree of saltiness water, and can be in fresh water breeding production, physiological ecological habit uniqueness, is the good species of high value of sea, freshwater aquiculture.Because of its inclined to one side phytophagy, less demanding to food proteins, aquaculture cost is lower, and economic worth is high, is coastal areas of southern China pond and the new important kind of cage culture in recent years, and cultivation DEVELOPMENT PROSPECT is wide.Because paralic environment worsens increasingly, cruel fishing is excessively captured, and has a strong impact on Scatophagus argus (Linnaeus) fishery resources procreation regeneration, and seedling quantity falls sharply year by year, and price is high, relies on wild seedling can not meet the needs of breeding production, has restricted aquaculture healthy and sustainable development.Carrying out the research and development of the artificial breeding technology of Scatophagus argus (Linnaeus), be not only conducive to protection, recovery and the sustainable utilization of natural resources, is also to solve the measure the most efficiently and effectively of current breeding production seedling scarcity.Realize the artificial propagation of Scatophagus argus (Linnaeus) seedling and the industrialization production of growing seedlings, need to be according to Scatophagus argus (Linnaeus) the adaptability to water environment and reproductive physiology ecosystem characterization, solve parent fish rearing and nutritional need, sexual gland accelerates the ripening, induces the technical problem such as reproduction and natural insemination.Relevant Scatophagus argus (Linnaeus) research data is few both at home and abroad, and germplasm raising technology research report lacks, and there is no can be for the practical and technical methods of artificial induction's reproduction.
Summary of the invention:
The object of the invention is to store up under condition and to regulate and necessary environment optimization by nutrition at net cage, impel Scatophagus argus (Linnaeus) sexual gland normal mature to grow, and under exogenous hormone effect, bring out the Scatophagus argus (Linnaeus) artificial induction natural spawning of egg mother cell Rapid development, maturation and natural spawning and fertilization and the method for fertilization, the method effect significantly, with stable, can adapt to the demand of Scatophagus argus (Linnaeus) aquaculture to artificial breeding seedling.
The method of Scatophagus argus (Linnaeus) artificial induction natural spawning of the present invention and fertilization, is characterized in that, comprises the following steps:
(1) in first 2 months of breeding period of Scatophagus argus (Linnaeus), select the parent population of physical health, under shading, sea area, offshore bay floating raft type net cage carries out fortification cultivation, mariculture area seawater velocity 5 ~ 20cm.s
-1, to feed with manufactured feed and high-quality marine alga, the total amount of throwing something and feeding the day of manufactured feed and high-quality marine alga is 2 ~ 4% of parent population gross mass;
(2) Scatophagus argus (Linnaeus) artificial induction natural spawning and fertilization: the Scatophagus argus (Linnaeus) parent population through nutrition improvement and reinforced cultivating in step (1), the 5 ~ August in reproduction period, choose the slightly completely parent population more than 2 ages of expansion of physical health, belly, egg mother cell is full, the tiny even compact of yolk protein, and ovum footpath 425 μ m ~ 500 μ m, body weight 400g are above, gonad development is to raun more than the IV phase and gently squeeze belly and have milky seminal fluid smoothness, more than body weight 200g ripe milter; At raun pectoral fin base portion injection hormone, hormone is the one in the middle of a class or b class, and its kind and dosage are: a parahormone: luteinizing hormone releasing hormone analog (Luteinzing Hormone Releasing Hormone-analogue, LHRH-A
2) 10 ~ 20.0 μ g/Kg BW(body weight), human chorionic gonadtropin (Human Chorionic Gonadotropin, HCG) 500 ~ 1500IU/Kg BW(body weight), maleic acid DOM (Domperidone, DOM) 2 ~ 5mg/Kg BW(body weight), injecting method is by luteinizing hormone releasing hormone analog (LHRH-A
2), human chorionic gonadtropin (HCG) and maleic acid DOM (DOM), inject according to above-mentioned dosage mix suspending in animal physiological salt solution; B parahormone, compound salmon gonadotropin releasing hormone analogues (Compound S-GnRHa for Injection) 10 ~ 15 μ g/Kg BW(body weight), compound salmon gonadotropin releasing hormone analogues (S-GnRHa) is also suspended in animal physiological salt solution according to its dosage, disposable injection; At the pectoral fin base portion injection a of milter parahormone, its hormone kind is identical with raun, dosage is 0 ~ 1/6 of raun, injecting method is identical with raun, and the reproduction environment of raun and milter is: in the net cage in sea area or breeding pond, and its water temperature is 25 DEG C ~ 29 DEG C, salinity is 22 ‰ ~ 28 ‰, in effector phase after injection, every day is to breeding water surface water spray in region, then milter ejaculation, raun spawning and fertilization.
The parent population of selecting physical health in described step (1), preferably the manufactured feed of throwing something and feeding is raised its juvenile fish stage.
The described injection of the pectoral fin base portion raun a parahormone, its injecting method is by luteinizing hormone releasing hormone analog (LHRH-A
2), human chorionic gonadtropin (HCG) and maleic acid DOM (DOM) according to above-mentioned dosage mix suspending in animal physiological salt solution, point 2 injections, shot to shot turnaround is 24 hours, injected dose is total amount 1/3 for the first time, is surplus for the second time.
The pectoral fin base portion injection a parahormone of described milter is preferably by luteinizing hormone releasing hormone analog (LHRH-A
2), human chorionic gonadtropin (HCG) and maleic acid DOM (DOM) according to above-mentioned dosage mix suspending in animal physiological salt solution, disposable injection.
The described injection of the pectoral fin base portion raun and milter hormone is being preferably 20mg/L nitrofurazone antibiotic and 150 ~ 200 × 10 as raun and the milter of parent population containing concentration
-6(v/v) in the seawater of ethylene glycol phenyl ether, anaesthetize.Alleviate the reaction of parent population distress and body injury and infect, be conducive to reproduction operation and keep parent population health.
Described manufactured feed is feed of the prior art, can buy from the market.
Described every day, preferably its water spraying time was 60 minutes to breeding water surface water spray in region, and during hormone ecbolic effect, sprinkle water 60 minutes every day to the water surface in breeding region, is conducive to stimulate the last maturation of raun egg mother cell and natural spawning fertilization.
Described animal physiological salt solution is mass fraction 0.9%NaCl solution, above-mentioned 4 kinds of hormones are uniformly mixed to form milky suspension after all adopting the preparation of 0.9%NaCl animal physiological salt solution by a certain percentage, temporarily refrigerate in curling stone for subsequent use after injection of hormone liquid matching while using or preparation.When use, fully shake up, avoid precipitation.
Scatophagus argus (Linnaeus) parent population enters breeding period in annual May conventionally, Scatophagus argus (Linnaeus) parent population nutrition improvement and hot housing are generally carried out in the time that water temperature starts rising, the February before Scatophagus argus (Linnaeus) parent population mating season the parent population of physical health is carried out to nutrient component improvement and hot housing.
Advantage of the present invention be parent population can be in the net cage of natural waters adjusting by nutrition optimization, Reproduction Conditions and exogenous hormone combine and impel Scatophagus argus (Linnaeus) gonad development and maturation, can be in net cage be laid eggs in mariculture area or cement breeding pond etc. propagate artificial induction's natural spawning and fertilization in region artificially.Female parent population is accelerated the ripening and efficiently reaches 89.1 ~ 100%, spawning rate 79.2 ~ 94.0%, and milter maturing rate reaches 100%, Gunther parent fish spawning sum reaches 48,000,000.The method is easy, convenient operation and control, technical costs is low, effect significantly and stable, and can more accurately judge according to gonad development state and sea area ecological condition maturation and spawning time of parent population, produces fertilized egg and biological study material are provided for Scatophagus argus (Linnaeus) seedling breeding.
Embodiment:
Following examples are of the present invention further illustrating, instead of limitation of the present invention.
Embodiment 1:
(1) parent population is that wild seed forms through artificial culture, and the juvenile fish stage manufactured feed of throwing something and feeding is main.In first 2 months of breeding period of Scatophagus argus (Linnaeus), select the parent population of physical health, under shading, sea area, offshore bay floating raft type net cage carries out fortification cultivation, mariculture area seawater velocity 5 ~ 20cm.s
-1between, feed sea, Guangdong board A series fish manufactured feed and the high-quality marine alga Enteromorpha produced with Yue Hai feed group of Guangdong Province, the mass ratio of manufactured feed and high-quality marine alga is 3:1, the total amount of throwing something and feeding the day of manufactured feed and high-quality marine alga is 2 ~ 4% of parent population gross mass;
(2) Scatophagus argus (Linnaeus) artificial induction natural spawning and fertilization: the Scatophagus argus (Linnaeus) parent population through nutrition improvement and reinforced cultivating in step (1), the May in reproduction period, choose slightly completely raun 46 tails in 2 ages of expansion of physical health, belly, egg mother cell is full, the tiny even compact of yolk protein, between ovum footpath 425 ~ 475 μ m, body weight 400g ~ 450g, the development of ovary has the ripe milter between milky seminal fluid smoothness, body weight 200g ~ 250g to more than the IV phase and gently squeezing belly; At raun pectoral fin base portion injection hormone, the kind of hormone and dosage are: luteinizing hormone releasing hormone analog (Luteinzing Hormone Releasing Hormone-analogue, LHRH-A
2) 10 μ g/Kg BW(body weight), human chorionic gonadtropin (Human Chorionic Gonadotropin, HCG) 1000IU/Kg BW(body weight), maleic acid DOM (Domperidone, DOM) 5mg/Kg BW(body weight); Injecting method is by luteinizing hormone releasing hormone analog (LHRH-A
2), human chorionic gonadtropin (HCG) and maleic acid DOM (DOM) according to above-mentioned dosage mix suspending in animal physiological salt solution, then divide 2 injections, shot to shot turnaround is 24 hours, and injected dose is total amount 1/3 for the first time, is surplus for the second time; At the pectoral fin base portion injection hormone of milter, its hormone kind is identical with raun, and dosage is raun 1/6, and injecting method is identical with raun, is luteinizing hormone releasing hormone analog (LHRH-A
2), human chorionic gonadtropin (HCG) and maleic acid DOM (DOM) be disposable injection, is to be 20mg/L nitrofurazone antibiotic and 150 × 10 as raun and the milter of parent population containing concentration at the pectoral fin base portion injection hormone of raun and milter
-6(v/v) injection after anesthesia in the seawater of ethylene glycol phenyl ether, can alleviate like this reaction of parent population distress and body injury and infect, and is conducive to reproduction operation and keeps parent population health.The breeding environment of raun and milter is: in the 40 order aperture sieve tulle casees of sea area, they are under 27.8 ~ 29.0 DEG C of sea area water temperatures, salinity 23 ‰ conditions, in effector phase after injection, spray water 60 minutes every day to the breeding region Intranet case water surface, after 24 hours effects, Oocyte Development is to diameter 450 ~ 500 μ m, injects for the second time 28 hours raun spawning and fertilizations after hormone.Reproduction in net cage is laid eggs in natural waters, raun spawning rate 82.7%, accelerating the ripening efficiently reaches 89.1%.
Embodiment 2:
(1) parent population is that wild seed forms through artificial culture, and the juvenile fish stage manufactured feed of throwing something and feeding is main.In first 2 months of breeding period of Scatophagus argus (Linnaeus), select the parent population of physical health, under shading, sea area, offshore bay floating raft type net cage carries out fortification cultivation, mariculture area seawater velocity 5 ~ 20cm.s
-1between, feed sea, Guangdong board A series fish manufactured feed and the high-quality marine alga Enteromorpha produced with Yue Hai feed group of Guangdong Province, the mass ratio of manufactured feed and high-quality marine alga is 3:1, the total amount of throwing something and feeding the day of manufactured feed and high-quality marine alga is 2 ~ 4% of parent population gross mass; (2) Scatophagus argus (Linnaeus) artificial induction natural spawning and fertilization: the Scatophagus argus (Linnaeus) parent population through nutrition improvement and reinforced cultivating in step (1), the May in reproduction period, choose slightly completely raun 48 tails in 3 ages of expansion of physical health, belly, egg mother cell is full, the tiny even compact of yolk protein, between ovum footpath 425 ~ 500 μ m, body weight 400g ~ 550g, the development of ovary has the ripe milter between milky seminal fluid smoothness, body weight 200g ~ 250g to more than the IV phase and gently squeezing belly; At raun pectoral fin base portion injection hormone, the kind of hormone and dosage are: luteinizing hormone releasing hormone analog (Luteinzing Hormone Releasing Hormone-analogue, LHRH-A
2) 20 μ g/Kg BW(body weight), human chorionic gonadtropin (Human Chorionic Gonadotropin, HCG) 1500IU/Kg BW(body weight), maleic acid DOM (Domperidone, DOM) 3mg/Kg BW(body weight); Injecting method is by luteinizing hormone releasing hormone analog (LHRH-A
2), human chorionic gonadtropin (HCG) and maleic acid DOM (DOM) according to above-mentioned dosage mix suspending in animal physiological salt solution, then divide 2 injections, shot to shot turnaround is 24 hours, and injected dose is total amount 1/3 for the first time, is surplus for the second time; At milter pectoral fin base portion injection hormone, its hormone kind is identical with raun, and dosage is raun 1/6, and injecting method is identical with raun, is luteinizing hormone releasing hormone analog (LHRH-A
2), human chorionic gonadtropin (HCG) and maleic acid DOM (DOM) be disposable injection, is to be 20mg/L nitrofurazone antibiotic and 150 × 10 as raun and the milter of parent population containing concentration at the pectoral fin base portion injection hormone of raun and milter
-6(v/v) injection after anesthesia in the seawater of ethylene glycol phenyl ether, can alleviate like this reaction of parent population distress and body injury and infect, and is conducive to reproduction operation and keeps parent population health.The reproduction environment of raun and milter is: in the 40 order aperture sieve tulle casees of sea area, it is under 26.0 ~ 28.0 DEG C of sea area water temperatures, salinity 25 ‰-28 ‰ condition, in effector phase after injection, to breeding region Intranet case water surface water spray 60 minutes, inject for the second time 20 hours raun spawning and fertilizations after hormone every day.Reproduction in net cage is laid eggs in natural waters, raun spawning rate 87.5%, accelerating the ripening efficiently reaches 91.7%.
Embodiment 3:
(1) parent population is that wild seed forms through artificial culture, and the juvenile fish stage manufactured feed of throwing something and feeding is main.In first 2 months of breeding period of Scatophagus argus (Linnaeus), select the parent population of physical health, under shading, sea area, offshore bay floating raft type net cage carries out fortification cultivation, mariculture area seawater velocity 5 ~ 20cm.s
-1between, feed sea, Guangdong board A series fish manufactured feed and the high-quality marine alga Enteromorpha produced with Yue Hai feed group of Guangdong Province, the mass ratio of manufactured feed and high-quality marine alga is 3:1, the total amount of throwing something and feeding the day of manufactured feed and high-quality marine alga is 2 ~ 4% of parent population gross mass;
(2) Scatophagus argus (Linnaeus) artificial induction natural spawning and fertilization: the Scatophagus argus (Linnaeus) parent population through nutrition improvement and reinforced cultivating in step (1), the June in reproduction period, choose slightly completely raun 34 tails in 2 ages of expansion of physical health, belly, egg mother cell is full, the tiny even compact of yolk protein, between ovum footpath 450 ~ 475 μ m, body weight 400g ~ 450g, the development of ovary has the ripe milter between milky seminal fluid smoothness, body weight 200g ~ 225g to more than the IV phase and gently squeezing belly; At raun pectoral fin base portion injection hormone, the kind of hormone and dosage are: luteinizing hormone releasing hormone analog (Luteinzing Hormone Releasing Hormone-analogue, LHRH-A
2) 15 μ g/Kg BW(body weight), human chorionic gonadtropin (Human Chorionic Gonadotropin, HCG) 1000IU/Kg BW(body weight), maleic acid DOM (Domperidone, DOM) 2mg/Kg BW(body weight); Injecting method is by luteinizing hormone releasing hormone analog (LHRH-A
2), human chorionic gonadtropin (HCG) and maleic acid DOM (DOM) according to above-mentioned dosage mix suspending in animal physiological salt solution, then divide 2 injections, shot to shot turnaround is 24 hours, and injected dose is total amount 1/3 for the first time, is surplus for the second time; Milter is not injected hormone, is to contain as the raun of parent population the nitrofurazone antibiotic and 200 × 10 that concentration is 20mg/L at the pectoral fin base portion injection hormone of raun
-6(v/v) injection after anesthesia in the seawater of ethylene glycol phenyl ether, so just can alleviate the reaction of parent population distress and body injury and infect, and is conducive to reproduction operation and keeps parent population health.The reproduction environment of raun and milter is: in the 40 order aperture sieve tulle casees of sea area, they are under 26.5 ~ 28.0 DEG C of sea area water temperatures, salinity 26 ‰ conditions, in effector phase after injection, to the net cage water surface water spray in breeding region 60 minutes, inject for the second time 30 hours raun spawning and fertilizations after hormone every day.Reproduction in net cage is laid eggs in natural waters, raun spawning rate 85.2%, accelerating the ripening efficiently reaches 94.1%.
Embodiment 4:
(1) parent population is that wild seed forms through artificial culture, and the juvenile fish stage manufactured feed of throwing something and feeding is main.In first 2 months of breeding period of Scatophagus argus (Linnaeus), select the parent population of physical health, under shading, sea area, offshore bay floating raft type net cage carries out fortification cultivation, mariculture area seawater velocity 5 ~ 20cm.s
-1between, feed sea, Guangdong board A series fish manufactured feed and the high-quality marine alga Enteromorpha produced with Yue Hai feed group of Guangdong Province, the mass ratio of manufactured feed and high-quality marine alga is 3:1, the total amount of throwing something and feeding the day of manufactured feed and high-quality marine alga is 2 ~ 4% of parent population gross mass;
(2) Scatophagus argus (Linnaeus) artificial induction natural spawning and fertilization: the Scatophagus argus (Linnaeus) parent population through nutrition improvement and reinforced cultivating in step (1), the June in reproduction period, choose slightly completely raun 53 tails in 2 ages of expansion of physical health, belly, egg mother cell is full, the tiny even compact of yolk protein, between ovum footpath 450 ~ 485 μ m, body weight 400g ~ 450g, the development of ovary has the ripe milter between milky seminal fluid smoothness, body weight 200g ~ 225g to more than the IV phase and gently squeezing belly; At raun pectoral fin base portion injection hormone, the kind of hormone and dosage are: luteinizing hormone releasing hormone analog (Luteinzing Hormone Releasing Hormone-analogue, LHRH-A
2) 10 μ g/Kg BW(body weight), human chorionic gonadtropin (Human Chorionic Gonadotropin, HCG) 500IU/Kg BW(body weight), maleic acid DOM (Domperidone, DOM) 3mg/Kg BW(body weight); Injecting method is by luteinizing hormone releasing hormone analog (LHRH-A
2), human chorionic gonadtropin (HCG) and maleic acid DOM (DOM) according to above-mentioned dosage mix suspending in animal physiological salt solution, then disposable injection; Milter is not injected hormone, is to contain as the raun of parent population the nitrofurazone antibiotic and 200 × 10 that concentration is 20mg/L at the pectoral fin base portion injection hormone of raun
-6(v/v) injection after anesthesia in the seawater of ethylene glycol phenyl ether, can alleviate like this reaction of parent population distress and body injury and infect, and is conducive to reproduction operation and keeps parent population health.The reproduction environment of raun and milter is: deeply for 1.7m, effective water body are 30m
3circular cement pit in breed, under 25.5 ~ 27.0 DEG C, the condition of salinity 25 ‰ ~ 27 ‰, in the effector phase after injection every day to water surface water spray in breeding region 60 minutes, 36 hours effector phases, raun spawning rate 79.2%, accelerating the ripening efficiently reaches 90.5%.
Embodiment 5:
(1) parent population is that wild seed forms through artificial culture, and the juvenile fish stage manufactured feed of throwing something and feeding is main.In first 2 months of breeding period of Scatophagus argus (Linnaeus), select the parent population of physical health, under shading, sea area, offshore bay floating raft type net cage carries out fortification cultivation, mariculture area seawater velocity 5 ~ 20cm.s
-1between, feed sea, Guangdong board A series fish manufactured feed and the high-quality marine alga Enteromorpha produced with Yue Hai feed group of Guangdong Province, the mass ratio of manufactured feed and high-quality marine alga is 3:1, the total amount of throwing something and feeding the day of manufactured feed and high-quality marine alga is 2 ~ 4% of parent population gross mass;
(2) Scatophagus argus (Linnaeus) artificial induction natural spawning and fertilization: the Scatophagus argus (Linnaeus) parent population through nutrition improvement and reinforced cultivating in step (1), the May in reproduction period, choose physical health, full 2 ages of belly summary expansion and raun totally 60 tails in 3 ages, egg mother cell is full, the tiny even compact of yolk protein, between ovum footpath 450 ~ 475 μ m, body weight 400g ~ 500g, the development of ovary has the ripe milter between milky seminal fluid smoothness, body weight 200g ~ 250g to more than the IV phase and gently squeezing belly; At raun pectoral fin base portion injection hormone, the kind of hormone and dosage are: compound salmon gonadotropin releasing hormone analogues 10 μ g/Kg BW; Injecting method is for to be suspended in compound salmon gonadotropin releasing hormone analogues in animal physiological salt solution, then disposable injection; Milter is not injected hormone, is to be 20mg/L nitrofurazone antibiotic and 200 × 10 as the raun of parent population containing concentration at the pectoral fin base portion injection hormone of raun
-6(v/v) injection after anesthesia in the seawater of ethylene glycol phenyl ether, can alleviate like this reaction of parent population distress and body injury and infect, and is conducive to reproduction operation and keeps parent population health.The growing environment of raun and milter is: in the 40 order aperture sieve tulle casees of sea area, it is in 28.0 ~ 28.5 DEG C of sea area water temperatures, salinity 22 ‰ ~ 24 ‰ condition, in effector phase after injection, spray water 60 minutes every day to the breeding region Intranet case water surface, 32 hours effector phases, raun spawning rate 86.7%, accelerating the ripening efficiently reaches 96.6%.
Embodiment 6:
(1) parent population is that wild seed forms through artificial culture, and the juvenile fish stage manufactured feed of throwing something and feeding is main.In first 2 months of breeding period of Scatophagus argus (Linnaeus), select the parent population of physical health, under shading, sea area, offshore bay floating raft type net cage carries out fortification cultivation, mariculture area seawater velocity 5 ~ 20cm.s
-1between, feed sea, Guangdong board A series fish manufactured feed and the high-quality marine alga Enteromorpha produced with Yue Hai feed group of Guangdong Province, the mass ratio of manufactured feed and high-quality marine alga is 3:1, the total amount of throwing something and feeding the day of manufactured feed and high-quality marine alga is 2 ~ 4% of parent population gross mass;
(2) Scatophagus argus (Linnaeus) artificial induction natural spawning and fertilization: the Scatophagus argus (Linnaeus) parent population through nutrition improvement and reinforced cultivating in step (1), the June in reproduction period, choose slightly completely raun totally 50 tails in 2 ages of expansion of physical health, belly, egg mother cell is full, the tiny even compact of yolk protein, between ovum footpath 450 ~ 500 μ m, body weight 400g ~ 450g, the development of ovary has the ripe milter between milky seminal fluid smoothness, body weight 200g ~ 250g to more than the IV phase and gently squeezing belly; At raun pectoral fin base portion injection hormone, the kind of hormone and dosage are: compound salmon gonadotropin releasing hormone analogues 15 μ g/KgBW; Injecting method is for to be suspended in compound salmon gonadotropin releasing hormone analogues in animal physiological salt solution, then disposable injection; Milter is not injected hormone, is to contain as the raun of parent population the nitrofurazone antibiotic and 200 × 10 that concentration is 20mg/L at the pectoral fin base portion injection hormone of raun
-6(v/v) injection after anesthesia in the seawater of ethylene glycol phenyl ether, can alleviate like this reaction of parent population distress and body injury and infect, and is conducive to reproduction operation and keeps parent population health.The reproduction environment of raun and milter is: in the 40 order aperture sieve tulle casees of sea area, it is in 28.0 ~ 29.0 DEG C of sea area water temperatures, salinity 22 ‰ ~ 25 ‰ condition, in effector phase after injection, spray water 60 minutes every day to the net cage water surface in breeding region, 30 hours effector phases, raun spawning rate 94.0%, accelerating the ripening efficiently reaches 100%.
Claims (6)
1. a method for Scatophagus argus (Linnaeus) artificial induction natural spawning and fertilization, is characterized in that, comprises the following steps:
(1) in first 2 months of breeding period of Scatophagus argus (Linnaeus), select the parent population of physical health, under shading, sea area, offshore bay floating raft type net cage carries out fortification cultivation, mariculture area seawater velocity 5 ~ 20cm.s
-1, to feed with manufactured feed and high-quality marine alga, the total amount of throwing something and feeding the day of manufactured feed and high-quality marine alga is 2 ~ 4% of parent population gross mass;
(2) Scatophagus argus (Linnaeus) artificial induction natural spawning and fertilization: the Scatophagus argus (Linnaeus) parent population through nutrition improvement and reinforced cultivating in step (1), the 5 ~ August in reproduction period, choose the slightly completely parent population more than 2 ages of expansion of physical health, belly, egg mother cell is full, the tiny even compact of yolk protein, and ovum footpath 425 μ m ~ 500 μ m, body weight 400g are above, gonad development is to raun more than the IV phase and gently squeeze belly and have milky seminal fluid smoothness, more than body weight 200g ripe milter; At raun pectoral fin base portion injection hormone, hormone is the one in the middle of a class or b class, its kind and dosage are: a parahormone: luteinizing hormone releasing hormone analog 10 ~ 20.0 μ g/Kg BW(body weight), human chorionic gonadtropin 500 ~ 1500IU/Kg BW(body weight), maleic acid DOM 2 ~ 5mg/Kg BW(body weight), injecting method be by luteinizing hormone releasing hormone analog, human chorionic gonadtropin and maleic acid DOM according to above-mentioned dosage mix suspending in animal physiological salt solution, inject; B parahormone, compound salmon gonadotropin releasing hormone analogues 10 ~ 15 μ g/Kg BW(body weight), compound salmon gonadotropin releasing hormone analogues is also suspended in animal physiological salt solution according to its dosage, disposable injection; At the pectoral fin base portion injection a of milter parahormone, its hormone kind is identical with raun, dosage is 0 ~ 1/6 of raun, injecting method is identical with raun, and the reproduction environment of raun and milter is: in the net cage in sea area or breeding pond, and its water temperature is 25 DEG C ~ 29 DEG C, salinity is 22 ‰ ~ 28 ‰, in effector phase after injection, every day is to breeding water surface water spray in region, then milter ejaculation, raun spawning and fertilization.
2. the method for Scatophagus argus (Linnaeus) artificial induction natural spawning according to claim 1 and fertilization, is characterized in that, the parent population of selecting physical health in described step (1), and the juvenile fish stage of its parent population the manufactured feed of throwing something and feeding is raised.
3. the method for Scatophagus argus (Linnaeus) artificial induction natural spawning according to claim 1 and fertilization, it is characterized in that, the described injection of the pectoral fin base portion raun a parahormone, its injecting method be by luteinizing hormone releasing hormone analog, human chorionic gonadtropin and maleic acid DOM according to above-mentioned dosage mix suspending in animal physiological salt solution, divide 2 injections, shot to shot turnaround is 24 hours, and injected dose is total amount 1/3 for the first time, is surplus for the second time.
4. the method for Scatophagus argus (Linnaeus) artificial induction natural spawning according to claim 1 and fertilization, it is characterized in that, the pectoral fin base portion injection a parahormone of described milter be by luteinizing hormone releasing hormone analog, human chorionic gonadtropin and maleic acid DOM according to above-mentioned dosage mix suspending in animal physiological salt solution, disposable injection.
5. the method for Scatophagus argus (Linnaeus) artificial induction natural spawning according to claim 1 and fertilization, it is characterized in that, the described injection of the pectoral fin base portion raun and milter hormone is to be 20mg/L nitrofurazone antibiotic and 150 ~ 200 × 10 as the raun of parent population and milter containing concentration
-6(v/v) anesthesia injection in the seawater of ethylene glycol phenyl ether.
6. the method for Scatophagus argus (Linnaeus) artificial induction natural spawning according to claim 1 and fertilization, is characterized in that, described every day, its water spraying time was 60 minutes to breeding water surface water spray in region.
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| CN107549064B (en) * | 2017-10-26 | 2019-08-02 | 上海市水产研究所 | Cement pit artificial fecundation method in a kind of Scatophagus argus (Linnaeus) room |
| CN107711621B (en) * | 2017-11-07 | 2019-12-24 | 华南师范大学 | Breeding method of scatophagus argus |
| CN108967278A (en) * | 2018-08-10 | 2018-12-11 | 深圳大学 | A kind of artificial fecundation method of Scatophagus argus (Linnaeus) |
| CN111000987B (en) * | 2019-12-18 | 2022-09-09 | 广东海洋大学 | Hormone kit for inducing spawning of scatophagus argus and artificial induced spawning and insemination method for obtaining fertilized eggs of scatophagus argus in batches |
| CN113303251A (en) * | 2021-04-21 | 2021-08-27 | 中国水产科学研究院东海水产研究所 | Efficient artificial reinforcement cultivation method for siganus longipes parents |
| CN113615608B (en) * | 2021-08-31 | 2023-01-03 | 陵水晨海种业有限公司 | Artificial breeding method of salangid |
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