CN104904636A - Method for artificially-induced spawning of little yellow croaker - Google Patents

Method for artificially-induced spawning of little yellow croaker Download PDF

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Publication number
CN104904636A
CN104904636A CN201510314903.1A CN201510314903A CN104904636A CN 104904636 A CN104904636 A CN 104904636A CN 201510314903 A CN201510314903 A CN 201510314903A CN 104904636 A CN104904636 A CN 104904636A
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China
Prior art keywords
little yellow
yellow croaker
induced spawning
parent population
injection
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CN104904636B (en
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詹炜
陈睿毅
楼宝
毛国民
徐冬冬
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Zhejiang Marine Fisheries Research Institute
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Zhejiang Marine Fisheries Research Institute
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Farming Of Fish And Shellfish (AREA)
  • Fodder In General (AREA)

Abstract

The invention discloses a method for artificially-induced spawning of little yellow croaker and belongs to the field of agricultural seawater fry rearing. The method includes that little yellow croaker of one year old and 100-200g in body weight is adopted as parent fishes for domestication strengthening; after strengthening, luteinizing hormone releasing hormone analogue number 2 (LHRH-A2) is adopted for dilution, the diluent is injected in one time, and the parent fishes can spawn in a cement pool after 24-26h. By the method, the little yellow croaker can smoothly spawn in the indoor cement pool, induced spawning rate can reach 75%, fertilized egg hatching rate reaches 80%, and needs on artificial fry cultivation of the little yellow croacker can be met.

Description

The induced spawning method of a kind of little yellow croaker
Invention field
The present invention relates to the induced spawning method of a kind of fish, belong to agriculture seawater offspring and breed field.
Background technology
Little yellow croaker ( pseudosciaena polyactisbleeker), have another name called little fresh, yellow croaker etc., be under the jurisdiction of Perciformes (Perciformes), Sciaenidae (Sciaenidae).Little yellow croaker is distributed widely in the offshore sea waters of East China Sea, the Huanghai Sea and the Bohai Sea and west bank, the Korea peninsula, belongs to warm warm nature near-bottom fish.Tool critical role in Chinese tradition sea fishery, once with large yellow Crocker, inkfish (Sepiella maindroni), hairtail being called " four large marine products ".But since the seventies in last century, by the impact of the factor such as overfishing and environmental deterioration, little yellow croaker output reduces year by year, force its adult fish price constantly to rise violently, the market price of current chilled fish reaches more than 80 yuan/kilogram, and supply falls short of demand.Meanwhile, the feature of little yellow croaker tool fast growth, is particularly suitable for propagating artificially.Therefore, the artificial induced spawning carrying out little yellow croaker is the primary problem solved of development artificial breeding and cultivation.
Summary of the invention
In order to solve the above-mentioned technical problem existed in prior art, the invention provides the induced spawning method of a kind of little yellow croaker, the method comprises reinforced cultivating and the injection of exogenous oxytocic drug of parent population, little yellow croaker parent population sexual gland is impelled to grow further, and at indoor water mud sump natural spawning, obtain high quality fertilized egg of scophthatmus.For the enforcement of little yellow croaker artificial breeding provides technical guarantee.
In order to realize above-mentioned object, present invention employs following technical scheme:
The induced spawning method of little yellow croaker, comprises the steps:
Step one, the indoor reinforced cultivating of parent population: parent population select 1 age fish, female and male ratio 2:1;
Step 2, artificial induced spawning: the oxytocic drug of employing is Leuteinizing hormone releasing hormone analogue No. 2 (LHRH-A 2), with medical 6% glucose injection dissolved dilution, injected dose is raun 1.25 μ g/kg, and milter dosage reduces by half; 5-6 point starts injection at dusk, and parenteral solution consumption of hastening parturition is every tail 0.3-0.5mL, disposable injection, covers full pond after injection with black puggaree, and keep surrounding environment quiet, after 24-26 hour, parent population can lay eggs in cement pit.
Further, in step one, reinforced cultivating initial time is the annual 2-3 month, and indoor water temperature reaches more than 10 DEG C.
Further, reinforced cultivating is as follows: water temperature 10-13 DEG C, illumination 1200LX, salinity 30-35, dissolved oxygen 5-8mg/L, day quantity of exchanged water 40%; Feed is that oyster meat adds squid block 1:1 ratio, and within one day, throw something and feed at twice, feeding volume accounts for 3% of the heavy body weight of parent population.
Further, the opportunity of hastening parturition in step 2 is: water temperature 14-16 DEG C, and milter extruding belly can flow out white seminal fluid, and raun anatomic observation sexual gland was developed to for IV phase; Before hastening parturition, the parent population MS-222 of 15-20 ppm concentration carries out drug anesthesia.
The present invention adopts with after technique scheme, and little yellow croaker can be made to lay eggs smoothly in indoor, solve indoor culture 1 age parent population cannot within the egg-laying period problem of natural spawning, simultaneously, spawning rate can reach 75%, and fertilization rate incubation rate reaches 80%, can meet the needs of little yellow croaker artificial breeding.
Embodiment
the invention will be further described below.
The induced spawning method of little yellow croaker of the present invention, comprises the steps:
1, the indoor reinforced cultivating of parent population
Reinforced cultivating initial time is the annual 2-3 month, and indoor water temperature reaches more than 10 DEG C.Parent population select 1 age fish, female and male ratio 2:1.Tighten management method: water temperature 10-13 DEG C, illumination 1200LX, salinity 30-35, dissolved oxygen 5-8mg/L, day quantity of exchanged water 40%; Feed is that oyster meat adds squid block 1:1 ratio, and within one day, throw something and feed at twice, feeding volume accounts for 3% of the heavy body weight of parent population.
2, artificial induced spawning
Hasten parturition opportunity: water temperature 14-16 DEG C, milter extruding belly can flow out white seminal fluid, and raun anatomic observation sexual gland was developed to for IV phase; Before hastening parturition, the parent population MS-222 of 15-20 ppm concentration carries out drug anesthesia.
The preparation of oxytocic drug and injection: the oxytocic drug of employing is Leuteinizing hormone releasing hormone analogue No. 2 (LHRH-A 2), with medical 6% glucose injection dissolved dilution, injected dose is raun 1.25 μ g/kg, and milter dosage reduces by half; 5-6 point starts injection at dusk, and parenteral solution consumption of hastening parturition is every tail 0.3-0.5mL, female and male ratio 2:1, disposable injection, covers full pond after injection with black puggaree, and keep surrounding environment quiet, after 24-26 hour, parent population can lay eggs in cement pit.
Embodiment
First arrival in March, 2015 in by the end of April, 2015, dodge in the indoor water mud sump of 20 squares, test site in Zhejiang Prov. Inst. of Marine Products west and carried out parent population reinforced cultivating and artificial induced spawning, the 200 tail parent populations of body weight 100-200g, obtain high-quality little yellow croaker fertilized egg 800mL altogether, are specifically implemented as follows:
1, the indoor reinforced cultivating of parent population
At the beginning of 3 months, indoor water temperature reaches more than 10 DEG C.Select 1 age fish little yellow croaker, female and male ratio 2:1 carries out reinforced cultivating.Tighten management method: water temperature 10-13 DEG C, illumination 1200LX, salinity 30-35, dissolved oxygen 5-8mg/L, day quantity of exchanged water 40%; Feed is that oyster meat adds squid block 1:1 ratio, and within one day, throw something and feed at twice, feeding volume accounts for 3% of the heavy body weight of parent population.
2, artificial induced spawning
Mid-April, water temperature reaches 14 DEG C, and milter extruding belly can flow out white seminal fluid, and get 3 tail raun anatomic observations, sexual gland was developed to for IV phase, can hasten parturition.Before hastening parturition, the parent population MS-222 of 15-20 ppm concentration carries out drug anesthesia.
The preparation of oxytocic drug and injection: the oxytocic drug of employing is Leuteinizing hormone releasing hormone analogue No. 2 (LHRH-A 2), with medical 6% glucose injection dissolved dilution, injected dose is raun 1.25 μ g/kg, and milter dosage reduces by half; 5-6 point starts injection at dusk, injection site is pectoral fin base portion, parenteral solution consumption of hastening parturition is every tail 0.3-0.5mL, female and male ratio 2:1, disposable injection, full pond is covered with black puggaree after injection, keep surrounding environment quiet, after 24-26 hour, parent population can lay eggs in cement pit, spawning rate is 75%, and fertilization rate incubation rate is 80%.

Claims (4)

1. the induced spawning method of little yellow croaker, comprises the steps:
Step one, the indoor reinforced cultivating of parent population: parent population select 1 age fish, female and male ratio 2:1;
Step 2, artificial induced spawning: the oxytocic drug of employing is Leuteinizing hormone releasing hormone analogue No. 2 (LHRH-A 2), with medical 6% glucose injection dissolved dilution, injected dose is raun 1.25 μ g/kg, and milter dosage reduces by half; 5-6 point starts injection at dusk, and parenteral solution consumption of hastening parturition is every tail 0.3-0.5mL, disposable injection, covers full pond after injection with black puggaree, and keep surrounding environment quiet, after 24-26 hour, parent population can lay eggs in cement pit.
2. the induced spawning method of little yellow croaker as claimed in claim 1, is characterized in that: in step one, reinforced cultivating initial time is the annual 2-3 month, and indoor water temperature reaches more than 10 DEG C.
3. the induced spawning method of little yellow croaker as claimed in claim 2, is characterized in that: reinforced cultivating is as follows: water temperature 10-13 DEG C, illumination 1200LX, salinity 30-35, dissolved oxygen 5-8mg/L, day quantity of exchanged water 40%; Feed is that oyster meat adds squid block 1:1 ratio, and within one day, throw something and feed at twice, feeding volume accounts for 3% of the heavy body weight of parent population.
4. the induced spawning method of little yellow croaker as claimed in claim 1, is characterized in that:
The opportunity of hastening parturition in step 2 is: water temperature 14-16 DEG C, and milter extruding belly can flow out white seminal fluid, and raun anatomic observation sexual gland was developed to for IV phase; Before hastening parturition, the parent population MS-222 of 15-20 ppm concentration carries out drug anesthesia.
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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106551180A (en) * 2016-10-26 2017-04-05 宁波大学 Feedstuff additive for sea water fish compositionss and application thereof
CN106577382A (en) * 2016-11-29 2017-04-26 浙江省海洋水产研究所 Pseudosciaenapolyactis family establishment and superior family selection method
CN106900604A (en) * 2017-02-23 2017-06-30 四川省农业科学院水产研究所 A kind of wild Hucho bleekeri induced spawning method
CN110810285A (en) * 2019-12-09 2020-02-21 浙江省农业科学院 Device and method for directional mating of small yellow croakers
CN112243896A (en) * 2020-10-20 2021-01-22 中国水产科学研究院淡水渔业研究中心 Artificial breeding method for freshwater grouper
CN112586408A (en) * 2020-11-19 2021-04-02 浙江省农业科学院 Method for identifying yellow croaker sexual maturity
CN114208733A (en) * 2022-01-12 2022-03-22 江苏省海洋水产研究所 Spawning induction method for parent fish of little yellow croaker
CN114731973A (en) * 2022-05-20 2022-07-12 浙江省农业科学院 Rapid breeding method for small yellow croakers

Families Citing this family (1)

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CN108142328B (en) * 2018-01-11 2020-05-05 浙江省海洋水产研究所 Early propagation and rapid seedling raising method for small yellow croakers

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CN101283677A (en) * 2008-06-04 2008-10-15 厦门大学 Artificial insemination process of large yellow croaker
CN101606502A (en) * 2009-01-05 2009-12-23 浙江海洋学院 A kind of method of breeding low temperature-resistant large yellow croakers

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CN101606502A (en) * 2009-01-05 2009-12-23 浙江海洋学院 A kind of method of breeding low temperature-resistant large yellow croakers

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Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106551180A (en) * 2016-10-26 2017-04-05 宁波大学 Feedstuff additive for sea water fish compositionss and application thereof
CN106577382A (en) * 2016-11-29 2017-04-26 浙江省海洋水产研究所 Pseudosciaenapolyactis family establishment and superior family selection method
CN106577382B (en) * 2016-11-29 2019-08-20 浙江省海洋水产研究所 A kind of building of little yellow croaker family and breeding superior families method
CN106900604A (en) * 2017-02-23 2017-06-30 四川省农业科学院水产研究所 A kind of wild Hucho bleekeri induced spawning method
CN110810285A (en) * 2019-12-09 2020-02-21 浙江省农业科学院 Device and method for directional mating of small yellow croakers
CN112243896A (en) * 2020-10-20 2021-01-22 中国水产科学研究院淡水渔业研究中心 Artificial breeding method for freshwater grouper
CN112586408A (en) * 2020-11-19 2021-04-02 浙江省农业科学院 Method for identifying yellow croaker sexual maturity
CN114208733A (en) * 2022-01-12 2022-03-22 江苏省海洋水产研究所 Spawning induction method for parent fish of little yellow croaker
CN114208733B (en) * 2022-01-12 2023-09-05 江苏省海洋水产研究所 Spawning induction method for parent fish of small yellow croaker
CN114731973A (en) * 2022-05-20 2022-07-12 浙江省农业科学院 Rapid breeding method for small yellow croakers

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