CN104904636A - Method for artificially-induced spawning of little yellow croaker - Google Patents
Method for artificially-induced spawning of little yellow croaker Download PDFInfo
- Publication number
- CN104904636A CN104904636A CN201510314903.1A CN201510314903A CN104904636A CN 104904636 A CN104904636 A CN 104904636A CN 201510314903 A CN201510314903 A CN 201510314903A CN 104904636 A CN104904636 A CN 104904636A
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- little yellow
- yellow croaker
- induced spawning
- parent population
- injection
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- 241000356842 Larimichthys polyactis Species 0.000 title claims abstract description 23
- 238000000034 method Methods 0.000 title claims abstract description 15
- 241000251468 Actinopterygii Species 0.000 claims abstract description 9
- 230000037396 body weight Effects 0.000 claims abstract description 6
- 239000004568 cement Substances 0.000 claims abstract description 6
- 239000012895 dilution Substances 0.000 claims abstract description 5
- 238000010790 dilution Methods 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- 229940090044 injection Drugs 0.000 claims description 16
- 239000007924 injection Substances 0.000 claims description 16
- 238000002347 injection Methods 0.000 claims description 16
- 230000032696 parturition Effects 0.000 claims description 12
- 229940079593 drug Drugs 0.000 claims description 11
- 239000003814 drug Substances 0.000 claims description 11
- 230000002196 ecbolic effect Effects 0.000 claims description 7
- 235000013601 eggs Nutrition 0.000 claims description 7
- 210000004907 gland Anatomy 0.000 claims description 5
- 230000001568 sexual effect Effects 0.000 claims description 5
- 206010002091 Anaesthesia Diseases 0.000 claims description 4
- 241000238366 Cephalopoda Species 0.000 claims description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- 241000237502 Ostreidae Species 0.000 claims description 4
- 210000001015 abdomen Anatomy 0.000 claims description 4
- DPWSRXJWCYEGIV-CWMZTGDLSA-N acetic acid;n-[(2s)-1-[[(2s)-1-[[(2s)-1-[[(2s)-1-[[(2r)-1-[[(2s)-1-[[(2s)-5-(diaminomethylideneamino)-1-[(2s)-2-(ethylcarbamoyl)pyrrolidin-1-yl]-1-oxopentan-2-yl]amino]-4-methyl-1-oxopentan-2-yl]amino]-1-oxopropan-2-yl]amino]-3-(4-hydroxyphenyl)-1-oxoprop Chemical compound CC(O)=O.CC(O)=O.CCNC(=O)[C@@H]1CCCN1C(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](C)NC(=O)[C@@H](NC(=O)[C@H](CO)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H](CC=1NC=NC=1)NC(=O)C1NC(=O)CC1)CC1=CC=C(O)C=C1 DPWSRXJWCYEGIV-CWMZTGDLSA-N 0.000 claims description 4
- 230000037005 anaesthesia Effects 0.000 claims description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 4
- 229940093181 glucose injection Drugs 0.000 claims description 4
- 239000005556 hormone Substances 0.000 claims description 4
- 229940088597 hormone Drugs 0.000 claims description 4
- 238000005286 illumination Methods 0.000 claims description 4
- 235000013372 meat Nutrition 0.000 claims description 4
- 229910052760 oxygen Inorganic materials 0.000 claims description 4
- 239000001301 oxygen Substances 0.000 claims description 4
- 235000020636 oyster Nutrition 0.000 claims description 4
- 239000003182 parenteral nutrition solution Substances 0.000 claims description 4
- 239000003488 releasing hormone Substances 0.000 claims description 4
- 210000000582 semen Anatomy 0.000 claims description 4
- 239000013535 sea water Substances 0.000 abstract description 2
- 238000005728 strengthening Methods 0.000 abstract 2
- 108700012941 GNRH1 Proteins 0.000 abstract 1
- 239000000579 Gonadotropin-Releasing Hormone Substances 0.000 abstract 1
- 239000003085 diluting agent Substances 0.000 abstract 1
- 230000012447 hatching Effects 0.000 abstract 1
- 230000000384 rearing effect Effects 0.000 abstract 1
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 241000276618 Perciformes Species 0.000 description 2
- 241001417494 Sciaenidae Species 0.000 description 2
- 230000004720 fertilization Effects 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 238000007726 management method Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 241001596954 Larimichthys Species 0.000 description 1
- 241001596950 Larimichthys crocea Species 0.000 description 1
- 241001663378 Sepiella maindroni Species 0.000 description 1
- 241001125843 Trichiuridae Species 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000017448 oviposition Effects 0.000 description 1
- 210000000006 pectoral fin Anatomy 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a method for artificially-induced spawning of little yellow croaker and belongs to the field of agricultural seawater fry rearing. The method includes that little yellow croaker of one year old and 100-200g in body weight is adopted as parent fishes for domestication strengthening; after strengthening, luteinizing hormone releasing hormone analogue number 2 (LHRH-A2) is adopted for dilution, the diluent is injected in one time, and the parent fishes can spawn in a cement pool after 24-26h. By the method, the little yellow croaker can smoothly spawn in the indoor cement pool, induced spawning rate can reach 75%, fertilized egg hatching rate reaches 80%, and needs on artificial fry cultivation of the little yellow croacker can be met.
Description
Invention field
The present invention relates to the induced spawning method of a kind of fish, belong to agriculture seawater offspring and breed field.
Background technology
Little yellow croaker (
pseudosciaena polyactisbleeker), have another name called little fresh, yellow croaker etc., be under the jurisdiction of Perciformes (Perciformes), Sciaenidae (Sciaenidae).Little yellow croaker is distributed widely in the offshore sea waters of East China Sea, the Huanghai Sea and the Bohai Sea and west bank, the Korea peninsula, belongs to warm warm nature near-bottom fish.Tool critical role in Chinese tradition sea fishery, once with large yellow Crocker, inkfish (Sepiella maindroni), hairtail being called " four large marine products ".But since the seventies in last century, by the impact of the factor such as overfishing and environmental deterioration, little yellow croaker output reduces year by year, force its adult fish price constantly to rise violently, the market price of current chilled fish reaches more than 80 yuan/kilogram, and supply falls short of demand.Meanwhile, the feature of little yellow croaker tool fast growth, is particularly suitable for propagating artificially.Therefore, the artificial induced spawning carrying out little yellow croaker is the primary problem solved of development artificial breeding and cultivation.
Summary of the invention
In order to solve the above-mentioned technical problem existed in prior art, the invention provides the induced spawning method of a kind of little yellow croaker, the method comprises reinforced cultivating and the injection of exogenous oxytocic drug of parent population, little yellow croaker parent population sexual gland is impelled to grow further, and at indoor water mud sump natural spawning, obtain high quality fertilized egg of scophthatmus.For the enforcement of little yellow croaker artificial breeding provides technical guarantee.
In order to realize above-mentioned object, present invention employs following technical scheme:
The induced spawning method of little yellow croaker, comprises the steps:
Step one, the indoor reinforced cultivating of parent population: parent population select 1 age fish, female and male ratio 2:1;
Step 2, artificial induced spawning: the oxytocic drug of employing is Leuteinizing hormone releasing hormone analogue No. 2 (LHRH-A
2), with medical 6% glucose injection dissolved dilution, injected dose is raun 1.25 μ g/kg, and milter dosage reduces by half; 5-6 point starts injection at dusk, and parenteral solution consumption of hastening parturition is every tail 0.3-0.5mL, disposable injection, covers full pond after injection with black puggaree, and keep surrounding environment quiet, after 24-26 hour, parent population can lay eggs in cement pit.
Further, in step one, reinforced cultivating initial time is the annual 2-3 month, and indoor water temperature reaches more than 10 DEG C.
Further, reinforced cultivating is as follows: water temperature 10-13 DEG C, illumination 1200LX, salinity 30-35, dissolved oxygen 5-8mg/L, day quantity of exchanged water 40%; Feed is that oyster meat adds squid block 1:1 ratio, and within one day, throw something and feed at twice, feeding volume accounts for 3% of the heavy body weight of parent population.
Further, the opportunity of hastening parturition in step 2 is: water temperature 14-16 DEG C, and milter extruding belly can flow out white seminal fluid, and raun anatomic observation sexual gland was developed to for IV phase; Before hastening parturition, the parent population MS-222 of 15-20 ppm concentration carries out drug anesthesia.
The present invention adopts with after technique scheme, and little yellow croaker can be made to lay eggs smoothly in indoor, solve indoor culture 1 age parent population cannot within the egg-laying period problem of natural spawning, simultaneously, spawning rate can reach 75%, and fertilization rate incubation rate reaches 80%, can meet the needs of little yellow croaker artificial breeding.
Embodiment
the invention will be further described below.
The induced spawning method of little yellow croaker of the present invention, comprises the steps:
1, the indoor reinforced cultivating of parent population
Reinforced cultivating initial time is the annual 2-3 month, and indoor water temperature reaches more than 10 DEG C.Parent population select 1 age fish, female and male ratio 2:1.Tighten management method: water temperature 10-13 DEG C, illumination 1200LX, salinity 30-35, dissolved oxygen 5-8mg/L, day quantity of exchanged water 40%; Feed is that oyster meat adds squid block 1:1 ratio, and within one day, throw something and feed at twice, feeding volume accounts for 3% of the heavy body weight of parent population.
2, artificial induced spawning
Hasten parturition opportunity: water temperature 14-16 DEG C, milter extruding belly can flow out white seminal fluid, and raun anatomic observation sexual gland was developed to for IV phase; Before hastening parturition, the parent population MS-222 of 15-20 ppm concentration carries out drug anesthesia.
The preparation of oxytocic drug and injection: the oxytocic drug of employing is Leuteinizing hormone releasing hormone analogue No. 2 (LHRH-A
2), with medical 6% glucose injection dissolved dilution, injected dose is raun 1.25 μ g/kg, and milter dosage reduces by half; 5-6 point starts injection at dusk, and parenteral solution consumption of hastening parturition is every tail 0.3-0.5mL, female and male ratio 2:1, disposable injection, covers full pond after injection with black puggaree, and keep surrounding environment quiet, after 24-26 hour, parent population can lay eggs in cement pit.
Embodiment
First arrival in March, 2015 in by the end of April, 2015, dodge in the indoor water mud sump of 20 squares, test site in Zhejiang Prov. Inst. of Marine Products west and carried out parent population reinforced cultivating and artificial induced spawning, the 200 tail parent populations of body weight 100-200g, obtain high-quality little yellow croaker fertilized egg 800mL altogether, are specifically implemented as follows:
1, the indoor reinforced cultivating of parent population
At the beginning of 3 months, indoor water temperature reaches more than 10 DEG C.Select 1 age fish little yellow croaker, female and male ratio 2:1 carries out reinforced cultivating.Tighten management method: water temperature 10-13 DEG C, illumination 1200LX, salinity 30-35, dissolved oxygen 5-8mg/L, day quantity of exchanged water 40%; Feed is that oyster meat adds squid block 1:1 ratio, and within one day, throw something and feed at twice, feeding volume accounts for 3% of the heavy body weight of parent population.
2, artificial induced spawning
Mid-April, water temperature reaches 14 DEG C, and milter extruding belly can flow out white seminal fluid, and get 3 tail raun anatomic observations, sexual gland was developed to for IV phase, can hasten parturition.Before hastening parturition, the parent population MS-222 of 15-20 ppm concentration carries out drug anesthesia.
The preparation of oxytocic drug and injection: the oxytocic drug of employing is Leuteinizing hormone releasing hormone analogue No. 2 (LHRH-A
2), with medical 6% glucose injection dissolved dilution, injected dose is raun 1.25 μ g/kg, and milter dosage reduces by half; 5-6 point starts injection at dusk, injection site is pectoral fin base portion, parenteral solution consumption of hastening parturition is every tail 0.3-0.5mL, female and male ratio 2:1, disposable injection, full pond is covered with black puggaree after injection, keep surrounding environment quiet, after 24-26 hour, parent population can lay eggs in cement pit, spawning rate is 75%, and fertilization rate incubation rate is 80%.
Claims (4)
1. the induced spawning method of little yellow croaker, comprises the steps:
Step one, the indoor reinforced cultivating of parent population: parent population select 1 age fish, female and male ratio 2:1;
Step 2, artificial induced spawning: the oxytocic drug of employing is Leuteinizing hormone releasing hormone analogue No. 2 (LHRH-A
2), with medical 6% glucose injection dissolved dilution, injected dose is raun 1.25 μ g/kg, and milter dosage reduces by half; 5-6 point starts injection at dusk, and parenteral solution consumption of hastening parturition is every tail 0.3-0.5mL, disposable injection, covers full pond after injection with black puggaree, and keep surrounding environment quiet, after 24-26 hour, parent population can lay eggs in cement pit.
2. the induced spawning method of little yellow croaker as claimed in claim 1, is characterized in that: in step one, reinforced cultivating initial time is the annual 2-3 month, and indoor water temperature reaches more than 10 DEG C.
3. the induced spawning method of little yellow croaker as claimed in claim 2, is characterized in that: reinforced cultivating is as follows: water temperature 10-13 DEG C, illumination 1200LX, salinity 30-35, dissolved oxygen 5-8mg/L, day quantity of exchanged water 40%; Feed is that oyster meat adds squid block 1:1 ratio, and within one day, throw something and feed at twice, feeding volume accounts for 3% of the heavy body weight of parent population.
4. the induced spawning method of little yellow croaker as claimed in claim 1, is characterized in that:
The opportunity of hastening parturition in step 2 is: water temperature 14-16 DEG C, and milter extruding belly can flow out white seminal fluid, and raun anatomic observation sexual gland was developed to for IV phase; Before hastening parturition, the parent population MS-222 of 15-20 ppm concentration carries out drug anesthesia.
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Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106551180A (en) * | 2016-10-26 | 2017-04-05 | 宁波大学 | Feedstuff additive for sea water fish compositionss and application thereof |
CN106577382A (en) * | 2016-11-29 | 2017-04-26 | 浙江省海洋水产研究所 | Pseudosciaenapolyactis family establishment and superior family selection method |
CN106900604A (en) * | 2017-02-23 | 2017-06-30 | 四川省农业科学院水产研究所 | A kind of wild Hucho bleekeri induced spawning method |
CN110810285A (en) * | 2019-12-09 | 2020-02-21 | 浙江省农业科学院 | Device and method for directional mating of small yellow croakers |
CN112243896A (en) * | 2020-10-20 | 2021-01-22 | 中国水产科学研究院淡水渔业研究中心 | Artificial breeding method for freshwater grouper |
CN112586408A (en) * | 2020-11-19 | 2021-04-02 | 浙江省农业科学院 | Method for identifying yellow croaker sexual maturity |
CN114208733A (en) * | 2022-01-12 | 2022-03-22 | 江苏省海洋水产研究所 | Spawning induction method for parent fish of little yellow croaker |
CN114731973A (en) * | 2022-05-20 | 2022-07-12 | 浙江省农业科学院 | Rapid breeding method for small yellow croakers |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108142328B (en) * | 2018-01-11 | 2020-05-05 | 浙江省海洋水产研究所 | Early propagation and rapid seedling raising method for small yellow croakers |
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Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106551180A (en) * | 2016-10-26 | 2017-04-05 | 宁波大学 | Feedstuff additive for sea water fish compositionss and application thereof |
CN106577382A (en) * | 2016-11-29 | 2017-04-26 | 浙江省海洋水产研究所 | Pseudosciaenapolyactis family establishment and superior family selection method |
CN106577382B (en) * | 2016-11-29 | 2019-08-20 | 浙江省海洋水产研究所 | A kind of building of little yellow croaker family and breeding superior families method |
CN106900604A (en) * | 2017-02-23 | 2017-06-30 | 四川省农业科学院水产研究所 | A kind of wild Hucho bleekeri induced spawning method |
CN110810285A (en) * | 2019-12-09 | 2020-02-21 | 浙江省农业科学院 | Device and method for directional mating of small yellow croakers |
CN112243896A (en) * | 2020-10-20 | 2021-01-22 | 中国水产科学研究院淡水渔业研究中心 | Artificial breeding method for freshwater grouper |
CN112586408A (en) * | 2020-11-19 | 2021-04-02 | 浙江省农业科学院 | Method for identifying yellow croaker sexual maturity |
CN114208733A (en) * | 2022-01-12 | 2022-03-22 | 江苏省海洋水产研究所 | Spawning induction method for parent fish of little yellow croaker |
CN114208733B (en) * | 2022-01-12 | 2023-09-05 | 江苏省海洋水产研究所 | Spawning induction method for parent fish of small yellow croaker |
CN114731973A (en) * | 2022-05-20 | 2022-07-12 | 浙江省农业科学院 | Rapid breeding method for small yellow croakers |
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