CN108142328A - A kind of morning of little yellow croaker is numerous and fast seedling-cultivating method - Google Patents
A kind of morning of little yellow croaker is numerous and fast seedling-cultivating method Download PDFInfo
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- CN108142328A CN108142328A CN201810027530.3A CN201810027530A CN108142328A CN 108142328 A CN108142328 A CN 108142328A CN 201810027530 A CN201810027530 A CN 201810027530A CN 108142328 A CN108142328 A CN 108142328A
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- 108010073521 Luteinizing Hormone Proteins 0.000 description 1
- 101800004705 Mesotocin Proteins 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The present invention relates to marine fish breeding and seedling technical fields, more particularly to a kind of morning of little yellow croaker is numerous and fast seedling-cultivating method, the processes such as fortification, ovulation fertilization, incubating oosperm, nursery cultivation are carried out including preparing parent fish pond and hatching cylinder, selection parent population.Pass through the early seedling-breeding method of the little yellow croaker of the present invention, the spawning and fertilization time of little yellow croaker can be made to shift to an earlier date 1 month compared with natural conditions, spawning rate is 88.2 ± 0.2%, rate of fertilization is 78.4 ± 0.1%, hatching rate is 76.7 ± 0.1%, the fry of the long 5cm of little yellow croaker body bring out the time under natural conditions cultivate compared with shorten 50 days, fry at the beginning of 6 months be averaged body length reach 7.8cm, survival rate improves 20%, realize the early emergence of little yellow croaker, it lists in advance, the seed price of little yellow croaker is high, economic benefit is good, it can alleviate demand of the market to big specification little yellow croaker product simultaneously, and provide the approach of protection East Sea little yellow croaker fishery resources.
Description
Technical field
The present invention relates to marine fish breeding and seedling technical fields, and in particular to a kind of morning of little yellow croaker is numerous and quick
Method for culturing seedlings.
Background technology
Little yellow croaker and Larimichthys crocea, hairtail, cuttlefish and " the four big marine products " that claim China, belong to Osteichthyes, Perciformes, sciaenoid
Section, yellow croaker category are the important economic fish of China coast.Its distribution is wider, be concentrated mainly on Bohai and Yellow Seas, the East Sea and
The Korea peninsula west bank sea area depth of water is less than the marine site of 100m.With the continuous development of fishery equipment and Fishing technology, little yellow croaker money
Source is caused little yellow croaker catch drastically to decline by the influence of the serious threat, the in addition factors such as environmental degradation of overfishing, small
Yellow croaker fishery population structure also occurs becoming younger, minimize, the sexal maturity variations such as in advance.In addition 1 age of little yellow croaker
Sexal maturity, Zhoushan Fishing Ground little yellow croaker annual mid-April start oviposition breeding, now growing by summer fishing moratorium phase
The weight of overwhelming majority little yellow croaker is no more than 100g/ items during city, and specification is smaller, leads to that the market price is relatively low, economic benefits are unknown
It is aobvious, and the market price that a small number of weight are more than the little yellow croaker of 150g/ items turns over 2 ~ 3 times compared with the former.Although existing little yellow croaker is complete now
The method of artificial propagation, but exist and take time and effort, production cost is big, survival rate is low, and seed Time To Market is long, big by seasonal effect
The shortcomings of, it is unfavorable for realizing that early emergence, in advance listing, seed price are high.Therefore cultivation fast growing, specification are larger manually supports
Little yellow croaker listing is grown, is the technical barrier for being badly in need of breaking through at present.
Chinese patent CN201410475021.9, patent name one kind breed ahead of time, cultivate big specification lip seed breeding side
Method is disclosed through the early method bred seedling and breed big specification skin-carp meat seed, including parent fish rearing, parent population accelerating, is manually urged
Production, artificial insemination and the processes such as hatching and seed rearing, but this method is only applicable to skin-carp meat, it is poor for applicability to little yellow croaker.
Invention content
Small for now most of little yellow croaker emergence specifications, the unconspicuous problem of economic benefit, the present invention provides a kind of small
The morning of yellow croaker is numerous and fast seedling-cultivating method, by foundation for the fast seedling-cultivating method of little yellow croaker, to improve the rule of little yellow croaker
Lattice shorten the seedling-growing time of little yellow croaker, realize that the early emergence, in advance listing, seed price of little yellow croaker are high, good so as to obtain
Economic benefit can alleviate demand of the market to big specification little yellow croaker product, can also provide protection East Sea little yellow croaker fishery resources
Approach.
The present invention provides following technical solution：
A kind of morning of little yellow croaker is numerous and fast seedling-cultivating method, includes the following steps：
（1）Parent fish pond and hatching cylinder are sterilized, is then rinsed well with sand filter seawater, reinjects sand filter seawater；
（2）The male parent population and female parent population for 60~100g of weight that early January selection body surface is fed without wound, normally are by 1:1 ratio
It is raised together in parent fish pond, and the artifical compound feed fed before mixed breeding is gradually substituted with clam worm；
（3）Mid-February water temperature is gradually heating to 15.5 DEG C to keep constant, quantitatively replaces seawater in parent fish pond daily and extends photograph
It is bright；
（4）Early March meets the requirements backward parent population internal injection and urges to the sexual gland maturity that parent population is checked during mid-March
The plain parenteral solution of production, makes parent population spawning and fertilization in parent fish pond；
（5）Fertilized eggs in collection parent fish pond are placed in conventional incubation in hatching cylinder and obtain fry；
（6）Ocean temperature in hatching cylinder is gradually increased to 28 DEG C after 10~15 days and kept, while with fry body length by hatching
Increase and carry out a point cylinder nursery.
As a kind of improvement of the method for the present invention, step（2）In in 5 days clam worm substitute artifical compound feed, clam worm completely
Mass ratio is launched daily with artifical compound feed to be followed successively by：1:4、2:3、1:1、4:1 and 5:0.
As a kind of improvement of the method for the present invention, step（3）In heating rate for 0.5 DEG C/day, daily with 16 DEG C of sand
Filter seawater replace parent fish pond in 30%~40% volume seawater, extend illumination to parent fish pond receive including natural lighting
The general ambient light time be 13.5 hours.
As a kind of improvement of the method for the present invention, step（4）The sexual gland maturation journey of middle parent population injectable oxytocin injections liquid
It spends and is：Raun sexual gland is full, the egg cell of visible particulate in sexual gland；Milter sexual gland is full to be creamy white, slight squeeze sexual gland
It can be seen that sperm overflows.
As a kind of improvement of the method for the present invention, step（4）Mesotocin parenteral solution is luteinizing hormone releasing hormone and people
The compound amino acid solution of human chorionic gonadtropin, a concentration of 1.2 μ g/kg of luteinizing hormone releasing hormone, human chorionic gonadotropin
Hormone levels are 500IU/kg, raun injection dosage 0.2ml, milter injection dosage 0.1mL.
As a kind of improvement of the method for the present invention, step（5）Breeding density of the middle fry in cylinder is hatched is 2 tails/L seas
Water.
As a kind of improvement of the method for the present invention, step（4）To parent population oxytocin injection parenteral solution and step（5）Collect by
The time interval of smart ovum is 36~48h.
As a kind of improvement of the method for the present invention, step（5）1 DEG C/day of heating rate, the method for dividing cylinder nursery are：Fry
The long 1cm time-divisions cylinder of body, breeding density are 1 tail/L seawater；Nursery body length is to 3cm time-division cylinders, 1 tail of breeding density/2L seawater.
As a kind of improvement of the method for the present invention, parent fish pond is indoor semi-buried cement pit, the pond deep 1.8 of parent fish pond~
2m, the depth of water 1m, Rong Shui 20m3, parent fish pond top is equipped with insulation film and headlamp, the area in insulation film covering pond face 4/5, illumination
Lamp power is 12W and layout density is 1/10m2。
As a kind of improvement of the method for the present invention, the salinity of sand filter seawater is 28 ‰~32 ‰.
The little yellow croaker fast seedling-cultivating method of the present invention realizes that little yellow croaker is early by early numerous reasonable cultivate with fry of little yellow croaker
Emergence.
Body surface selected to be fed without wound, normally in early January, move active little yellow croaker hero parent population and female parent population hydrophilic
Fortification is carried out in pond.Gradually replace the artifical compound feed fed before mixed breeding with clam worm during fortification, so as to
Enhance parent population constitution, promote little yellow croaker growth, carry out getting fat weightening.In mid-February with the heating rate of 0.5 DEG C/day by parent population
The seawater in pond is warming up to 15.5 DEG C of further hot housing 10~15 days, and in early March to during the middle ten days, little yellow croaker sexual gland reaches
It hastens parturition to maturation, collects fertilized eggs and hatched.Natural conditions offshore sea waters in lower January water temperature is 10 DEG C, and little yellow croaker protects
Hold certain active degree, into 2 months after seawater water temperature be reduced to 8 DEG C, movement and the feed further reduction of little yellow croaker, no
Conducive to the fortification of little yellow croaker, therefore select to carry out little yellow croaker parent population fortification nursing in January.Select little yellow croaker parent population
Weight for 60~100g, the small little yellow croaker constitution of weight is relatively weak, and weight is excessive, may be 2 age fishes.During by one section
Between feed strengthen after in mid-February parent fish pond water temperature is warming up to 15.5 DEG C with the gradient of 0.5 DEG C/day, made by slowly heating up
The body of little yellow croaker gradually adapts to water temperature and reaches full growth, and extends by light application time and the continuation nutrition of 10~15 days is strong
When changing to early or mid March, the sexual gland of little yellow croaker reaches ripe, and then oxytocin injection parenteral solution progress little yellow croaker hastens parturition and is fertilized
Form fertilized eggs.With little yellow croaker under natural conditions compared with mid-April starts spawning and fertilization, fertilization time shifts to an earlier date 1 month or so.
Oxytocin injections liquid used is luteinizing hormone releasing hormone（Lhrh-A2）With human chorionic gonadotrophin（HGG）Compound
Amino acid solution, wherein HCG directly stimulate the sexual gland of little yellow croaker to discharge follicular stimulating hormone and lutropin, promote ovum and essence
The maturation of son and discharge, Lhrh-A2 act on ovulation rate by the hypophysis to little yellow croaker, and Lhrh-A2 and HGG compoundings can be effective
The spawning rate of the insensitive parent population of exogenous hormone is improved, the spawning rate of the little yellow croaker obtained through the above process is 88%, and rate of fertilization is
78%, it is 76% that fertilized eggs are transferred to the hatching rate hatched in hatching cylinder.Nature seawater temperature reaches 11 after hatching 10~15 days
DEG C, at this time with ocean temperature in the rate of 1 DEG C/day heating hatching cylinder to 28 DEG C, the time is mid-April, keeps little Huang at 28 DEG C
The fry fast-growth of fish, and carry out a point cylinder in growth course and cultivate.By above-mentioned morning is numerous and seedling raising process, during early June
The fry of little yellow croaker is averaged a length of 7.8cm of body, and compared under natural conditions, the time that brings out of the long 5cm fries of body shortens 50 days, deposits
Motility rate improves 20%, so as to shorten the seedling-growing time of little yellow croaker, realizes that the early emergence, in advance listing, seed price of little yellow croaker are high
Target.
Beneficial effects of the present invention are as follows：
The present invention provides a kind of artificial early seedling-breeding method for little yellow croaker, the spawning and fertilization time of little yellow croaker and natural item
For part compared to 1 month in advance, spawning rate was 88.2 ± 0.2%, and rate of fertilization is 78.4 ± 0.1%, and hatching rate is 76.7 ± 0.1%, June
The fry in the first tenday period of a month body length that is averaged reaches 7.8cm, and the fry of the long 5cm of little yellow croaker body brings out the time with being cultivated under natural conditions compared to contracting
50 days short, survival rate improves 20%, the target early emerge so as to fulfill little yellow croaker, listed in advance, and the seed price of little yellow croaker is high,
Economic benefit is good, while can alleviate demand of the market to big specification little yellow croaker product, and provides protection East Sea little yellow croaker fishing
The approach of industry resource.
Description of the drawings
Fig. 1 is the Technology Roadmap of the method for the present invention.
Fig. 2 is the fry body length comparison diagram at a temperature of different cultivations.
Fig. 3 is the statistics diagram that different cultivation temperature influence fry body length.
Specific embodiment
Just the specific embodiment of the present invention is described further below.
Unless otherwise instructed, the raw material employed in the present invention is commercially available or commonly used in the art, such as
Without special instruction, the method in following embodiments is the conventional method of this field.
The salinity of sand filter seawater used in the method for the present invention is 28 ‰~32 ‰.
Parent fish pond used in the method for the present invention is located at the western pavilion fishery science and technology island of Zhoushan sea area, for indoor semi-buried cement
Pond, the pond deep 1.8~2m, the depth of water 1m, Rong Shui 20m of parent fish pond3, equipped with insulation film and headlamp, insulation film covers at parent fish pond top
The area in Gai Chi faces 4/5, insulation film are two layers of black mulch film, and headlamp power is 12W and layout density is 1/10m2。
Heating unit used in the method for the present invention is heating rod, and layout density is 1 heating rod/2m in parent fish pond2。
In the method for the present invention to parent population oxytocin injection parenteral solution with collect fertilized eggs time interval be 36~48h.
As shown in Figure 1, the technology path of the method for the present invention is：Early January select little yellow croaker hero parent population and female parent population according to
Quantity 1:1 raises together in parent fish pond, keeps seawater salinity 28 ‰~32 ‰ in parent fish pond, and fortification is carried out by feeding clam worm
Culture, being then warming up to 15.5 DEG C and extended light application time and reach 13.5 hours water temperature with the velocity gradient of 0.5 DEG C/day makes parent
Fish forms Zao Fan groups, shifts to an earlier date fertilization in 1 month with nature compared with, by fertilized eggs in cylinder is hatched hatching, cultivating, and will be extra large
Coolant-temperature gage is warming up to 28 DEG C of cultivations with the velocity gradient of 1 DEG C/day, is cultivated through two months to early or mid June, nature seawater temperature reaches
Aquaculture pond routine culture is transferred to during to 28 DEG C and forms little yellow croaker adult fish, the specification of little yellow croaker adult fish is big compared with natural conditions,
Then movement is may select to enliven, feed normal little yellow croaker raun and milter as little yellow croaker hero parent population and female parent population in parent
Fortification culture is carried out in fishpond again, carry out next round little yellow croaker is early numerous and fast seedling growing is cultivated.
Embodiment
A kind of morning of little yellow croaker is numerous and fast seedling-cultivating method, includes the following steps：
（1）Annual late December prepares parent fish pond and hatching cylinder, and 700mg/L sodium hypochlorite of splashing in parent fish pond and hatching cylinder is molten
Then liquid disinfectant is rinsed well with sand filter seawater, reinject sand filter seawater；
（2）Early January Zhoushan sea area ocean temperature is 10 DEG C, and body surface is selected to feed and move active hero parent without wound, normally
Fish and female parent population press 1:1 ratio is raised together in parent fish pond, parent population weight 80g/ items, 600 tails of breeding density/parent fish pond, with clam worm 5
The artifical compound feed fed before mixed breeding is gradually substituted in it, clam worm launches mass ratio with artifical compound feed and is followed successively by daily：
1:4、2:3、1:1、4:1 and full clam worm, it is satiated with food feeds 3 times daily, feed that total amount is little yellow croaker weight 1.5%；
（3）Mid-February, Zhoushan sea area ocean temperature was 8 DEG C, and water temperature is warming up to 15.5 DEG C through 15 days with the rate of 0.5 DEG C/day
It keeps constant, replaces the seawater of 30% volume in parent fish pond with 16 DEG C of sand filter seawater daily, and open headlamp extension illumination to make
The general ambient light time including natural lighting that parent fish pond receives is 13.5 hours；
（4）Mid-March checks the sexual gland maturity of parent population, when raun sexual gland is full, the egg cell of visible particulate in sexual gland
With milter sexual gland is full is creamy white, slight squeeze sexual gland visible sperm meets requirement of hastening parturition when overflowing, first day 18:When 00
To parent population pectoral fin base portion oxytocin injection parenteral solution, 6 in third day:00 collects fertilized eggs, and oxytocin injections liquid is promotees corpus luteum
The compound amino acid solution of hormone-releasing hormone and human chorionic gonadotrophin, a concentration of 1.2 μ g/ of luteinizing hormone releasing hormone
Kg, a concentration of 500IU/kg of human chorionic gonadotrophin, raun injection dosage are 0.2mL, and milter injection dosage is 0.1mL；
（5）The fertilized eggs that parent fish pond is collected are placed in conventional incubation in hatching cylinder and obtain fry, fry breeding density is 2 tails/L seas
Water；
（6）After hatching 10 days, the temperature for hatching seawater in cylinder is risen into 28 DEG C of cultivation fries, while with fry body with 1 DEG C/day
Long increase and carry out a point cylinder, method is：The long 1cm time-divisions cylinder of fry body is primary, and breeding density is 1 tail/L seawater；Nursery body is long
It is primary to 3cm time-division cylinders, 1 tail of breeding density/2L seawater；
（7）June, fry was gone to the conventional cultivation change of aquaculture pond progress by the water temperature of Zhoushan sea area nature seawater up at 28 DEG C
Into little yellow croaker adult fish, and can as next little yellow croaker early numerous and fast seedling growing parent population source.
The oviposition of the little yellow croaker obtained through the above method and fertilization time shift to an earlier date 1 month compared with natural conditions, spawning rate
It is 88.2 ± 0.2%, rate of fertilization is 78.4 ± 0.1%, and hatching rate is 76.7 ± 0.1%, when the fry of the long 5cm of little yellow croaker body brings out
Between with being cultivated under natural conditions compared to shortening 50 days, the fry of the early June body that is averaged is grown and reaches 7.8cm, survival rate raising 20%.
It should be noted that in the above-described embodiments and being not limited to the above embodiments, step（2）Middle parent fish pond launches parent population
Other suitable breeding density be less than 600 tails/parent fish pond and not less than 400 tails/parent fish pond, other suitable parent population weight are
Less than 80g/ items but not less than 60g/ items or more than 80g/ items but no more than 100g/ items, suitable are satiated with food other daily feed
Number is 2 times；Step（3）In to replace other appropriate volumes of parent fish pond seawater daily be more than 30% but no more than 40%；Step
（4）In other check parent populations sexual gland maturity time be early March；Little yellow croaker early breeding seedling is carried out using above-mentioned parameter
The difference of the spawning rate of gained parent population, the statistical value of fertility rate and hatchability and embodiment is no more than 2%.Step（6）Middle hatching 10
Nature seawater water temperature after~15 days is 11 DEG C, chooses 11 days, 12 days, 13 days, 14 days or the 15 days speed with 1 DEG C/day after hatching
Rate heating seawater is no more than 1% with influence of 10 days heating seawater to fry growth after hatching.Step（7）In can also be in nature
Fry is transferred to aquaculture pond when the water temperature of seawater reaches 22 DEG C and carries out conventional cultivation.
The influence that fry rearing temperature grows fish body
Control group：With above-described embodiment（Temperature is cultivated after hatching 10 with the heating of 1 DEG C/day rate up to 28 DEG C and to keep）Difference
It is in ocean temperature is synchronous with the holding of nature seawater temperature in hatching cylinder after fry hatching；
16 DEG C of groups：With above-described embodiment（Temperature is cultivated after hatching 10 with the heating of 1 DEG C/day rate up to 28 DEG C and to keep）Difference
It is in ocean temperature in hatching cylinder is risen to 16 DEG C with 1 DEG C/day after 10 days and keeps cultivating fry by fry hatching；
22 DEG C of groups：Above-described embodiment（Temperature is cultivated after hatching 10 with the heating of 1 DEG C/day rate up to 28 DEG C and to keep）Difference
It is, ocean temperature in hatching cylinder is risen to 22 DEG C with 1 DEG C/day after 10 days and keeps cultivating fry by fry hatching.
Fig. 2 is shown in individual body length comparison of the fry of embodiment and control group, 16 DEG C of groups and 22 DEG C of groups after hatching 40 days, no
Statistical analysis with the long feature of body for cultivating temperature group is shown in Fig. 3, and statistical method uses one-way analysis of variance（one-
way ANOVA）Duncan ' the s post hoc detections followed.
From figure 1 it appears that hatching is warming up to the long longest of body of the little yellow croaker fry individual of 28 DEG C of cultivations after 10 days,
Secondly the secondary fry individual to be warming up to 22 DEG C of cultivations is the fry individual for keeping nature seawater temperature, is warming up to 16 DEG C of cultivations
Fry individual body length it is minimum.
Figure it is seen that the body length of the little yellow croaker fry of 28 DEG C of cultivations is significantly higher than the little yellow croaker fry of 22 DEG C of cultivations
Body it is long；The body that the body length of the little yellow croaker fry of 22 DEG C of cultivations is significantly higher than control group fry is long；The body length of control group fry is aobvious
It writes long higher than the body of the little yellow croaker fry of 16 DEG C of cultivations；Different lowercases on error bar represent statistics in P<0.05
It is worth variant.
Claims (10)
1. a kind of morning of little yellow croaker is numerous and fast seedling-cultivating method, include the following steps：
（1）Parent fish pond and hatching cylinder are sterilized, is then rinsed well with sand filter seawater, reinjects sand filter seawater；
（2）The male parent population and female parent population for 60~100g of weight that early January selection body surface is fed without wound, normally are by 1:1 ratio
It is raised together in parent fish pond, and the artifical compound feed fed before mixed breeding is gradually substituted with clam worm；
（3）Mid-February water temperature is gradually heating to 15.5 DEG C to keep constant, quantitatively replaces seawater in parent fish pond daily and extends photograph
It is bright；
（4）Early March meets the requirements backward parent population internal injection and urges to the sexual gland maturity that parent population is checked during mid-March
The plain parenteral solution of production, makes parent population spawning and fertilization in parent fish pond；
（5）Fertilized eggs in collection parent fish pond are placed in conventional incubation in hatching cylinder and obtain fry；
（6）Ocean temperature in hatching cylinder is gradually increased to 28 DEG C after 10~15 days and kept, while with fry body length by hatching
Increase and carry out a point cylinder nursery.
2. the morning of little yellow croaker according to claim 1 is numerous and fast seedling-cultivating method, which is characterized in that step（2）In in 5 days
Clam worm substitutes artifical compound feed completely, and clam worm launches mass ratio with artifical compound feed and is followed successively by daily：1:4、2:3、1:1、
4:1 and 5:0.
3. the morning of little yellow croaker according to claim 1 is numerous and fast seedling-cultivating method, which is characterized in that step（3）In liter
Warm rate is 0.5 DEG C/day, replaces the seawater of 30%~40% volume in parent fish pond with 16 DEG C of sand filter seawater daily, extends illumination
The general ambient light time including natural lighting received to parent fish pond is 13.5 hours.
4. the morning of little yellow croaker according to claim 1 is numerous and fast seedling-cultivating method, which is characterized in that step（4）Middle parent population
The sexual gland maturity of injectable oxytocin injections liquid is：Raun sexual gland is full, the egg cell of visible particulate in sexual gland；Milter
Sexual gland is full to be creamy white, and the visible sperm of slight squeeze sexual gland overflows.
5. the morning of the little yellow croaker according to claim 1 or 4 is numerous and fast seedling-cultivating method, which is characterized in that step（4）In urge
The plain parenteral solution of production is luteinizing hormone releasing hormone and the compound amino acid solution of human chorionic gonadotrophin, and luteotropin discharges
Hormone concentration be 1.2 μ g/kg, a concentration of 500IU/kg of human chorionic gonadotrophin, raun injection dosage 0.2ml, milter note
Penetrate dosage 0.1mL.
6. the morning of little yellow croaker according to claim 1 is numerous and fast seedling-cultivating method, which is characterized in that step（5）Middle fry
Breeding density in cylinder is hatched is 2 tails/L seawater.
7. the morning of the little yellow croaker according to step 1 or 4 or 6 is numerous and fast seedling-cultivating method, which is characterized in that step（4）To parent
Fish oxytocin injection parenteral solution and step（5）The time interval for collecting fertilized eggs is 36~48h.
8. the morning of little yellow croaker according to claim 1 is numerous and fast seedling-cultivating method, which is characterized in that step（6）Heating
1 DEG C/day of rate, the method for dividing cylinder nursery are：The long 1cm time-divisions cylinder of fry body, breeding density are 1 tail/L seawater；Nursery body grow to
3cm time-division cylinders, 1 tail of breeding density/2L seawater.
9. the morning of little yellow croaker according to claim 1 is numerous and fast seedling-cultivating method, which is characterized in that parent fish pond is indoor half
Buried cement pit, the pond deep 1.8~2m, the depth of water 1m, Rong Shui 20m of parent fish pond3, insulation film and headlamp are equipped at the top of parent fish pond,
Insulation film covers the area in pond face 4/5, and headlamp power is 12W and layout density is 1/10m2。
10. little yellow croaker according to claim 1 is early numerous and fast seedling-cultivating method, which is characterized in that the salinity of sand filter seawater
It is 28 ‰~32 ‰.
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