CN1012333B - Artificial breeding method of trachidermus fasciatus - Google Patents
Artificial breeding method of trachidermus fasciatusInfo
- Publication number
- CN1012333B CN1012333B CN87101107A CN87101107A CN1012333B CN 1012333 B CN1012333 B CN 1012333B CN 87101107 A CN87101107 A CN 87101107A CN 87101107 A CN87101107 A CN 87101107A CN 1012333 B CN1012333 B CN 1012333B
- Authority
- CN
- China
- Prior art keywords
- parent population
- fish
- percentage
- sungkiang weever
- nai
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Farming Of Fish And Shellfish (AREA)
- Fodder In General (AREA)
Abstract
The present invention relates to a series of methods which are formed by that ecological environment is simulated under the condition of laboratories, Songjiang weever oviposits, impregnates and incubates in rooms, incubated larva fishes in different development stages are fed artificially, etc. The normal development rate of incubated larva fry is up to 86.0%, and the misshapen rate is up to 14.0%. After vitellicle is fully absorbed, the larva fry can fully exist on taking food from the outside. The present invention provides practice basis for industrial production of the Songjiang weever.
Description
The present invention is under laboratory condition, and emulated ecological environment makes Sungkiang weever in the indoor serial of methods of laying eggs, be fertilized, hatch and the prelarva that is in development in different stages after hatching being carried out artificial feeding etc.
Sungkiang weever, be four-gill perch again, be a kind of cuisines fish of China, plain in history well-known, be celebrated so that fine and tender taste is delicious, past all produces in the China's Coastal Areas rivers and creeks, because the variation of ecotope, output day subtracts in recent years, and some is historical abounds with the area and also be close to as Songjiang and die out, must adopt an effective measure, in time rescue.And artificial propagation ought be the most thorough effective solution.
1974, units such as plant of Songjiang County She Shan people's commune once carried out the artificial propagation test in She Shan plant.It is reported and picture shown in (" artificial propagation test of Sungkiang weever (four-gill perch) " 1974 the 6th phases of aquatic science and technology information), the young seedling great majority that obtain are deformity, are considered to belong to normal its afterbody of young seedling and also are bent upwards.The whole young seedling survival period of hatching like this is no more than 5 at most, does not all absorb the phase to yolk sac, does not see that more it gets food.According to list of references " investigation of the Sungkiang weever reproductive habit " report of (1980 the 4th the 1st phases of volume of aquatic product journal), normal young seedling form is that afterbody is straight shape.So before the present invention, the artificial propagation of Sungkiang weever is not seen successfully.
The objective of the invention is under laboratory condition, emulated ecological environment is bred Sungkiang weever with manual method.
Content of the present invention is a kind of artificial fecundation method of Sungkiang weever, comprises that raising, the parent population of parent population gonadally accelerated the ripening, the raising of the hastening parturition of parent population, fertilized egg hatching and young seedling.The raising condition of parent population be pH between 6.2～7.2, illumination is at 400～5000Lux, temperature is controlled at 2～15 ℃.The gonad of parent population accelerates the ripening increases sodium chloride salinity 0.02～0.25%(percentage by weight every day), add to 1～3.2%(percentage by weight), add sodium iodide (NaI) 5.0 * 10 simultaneously every day
-4μ g/ml, to concentration be 0.001～0.1 μ g/ml or potassium iodide (KI) 5.5 * 10
-4μ g/ml is to concentration 0.002～0.2 μ g/ml.Parent population injection hormone is in order to hasten parturition.Fertilized egg hatching sodium chloride salinity is at 1.7～3.2%(percentage by weight), temperature is at 4～16 ℃, and pH is 6.2～7.2, and illumination is 400～2000Lux, and water oxygen level is 4-6mg/L.Young seedling is hatched and was fed with artificial feed in back three days, adds then and feeds maritime protozoa, maritime monoplast green alga class, after young seedling yolk sac all absorbs, also to add the naupiar larva and the monoplast green alga of feeding wheel animalcule, cladocera and scratching sufficient class, when young seedling grows to 2cm body length, feed hatching, shrimp seedling gradually.
The inventor thinks that the reproductive characteristic of Sungkiang weever both had been different from freshwater fish, has differently with general ocean fish again, and its unique physiological ecological requirement is arranged.Its mesohaline variation is the important key of Sungkiang weever gonad maturation, and trace element is the key factor that promotes Sungkiang weever gonad maturation in the seawater, and essential ecotope is the assurance of the normal breeding of Sungkiang weever.As starting with from changing the water salinity gradually, necessary trace element is provided, be aided with the injection of the related hormones of various promotion gonad maturations and ovulation, and the simulate natural ecological environment of in experimentation, trying one's best, can obtain the sperm and the ovum of normal mature, carry out artificial insemination, and obtain the young seedling of normal hatching.If find out water quality and plankton situation in the spawning ground, then further solve young seedling starter diet and raise into the adult fish problem and also belong to possibility.Therefore, since winter in 1981, the inventor studied the artificial propagation of Sungkiang weever.Through the test in 6 years, now solved and laid eggs, fertilization, hatching and a series of problems that the prelarva that is in development in different stages is raised etc.
Serial of methods of the present invention roughly can be divided into five stages: the raising of parent population, parent population is gonadal accelerates the ripening, parent population hasten parturition fertilized egg hatching, the raising of young seedling.Following segmentation is narrated it.
One, parent population is raised: simulated the living condition of Sungkiang weever under extraneous natural conditions.For example gonadal maturation of parent population and ovulation have certain pH, and the requirement of temperature and illumination must be met.PH requires at 6.2-7.2, and the suitableeest is 6.5.Illumination is when being lower than 400Lux, and parent population is difficult to lay eggs, and breadboard illumination condition is 400-5000Lux.Because Sungkiang weever is the low temperature season fish that lay eggs, and gropes through the laboratory, temperature should be controlled at 2-15 ℃, and parent population is very easily dead more than 15 ℃ the time, wherein especially with milter for very.
Two, parent population is gonadal accelerates the ripening: experiment showed, that the salinity increase only can cause that Sungkiang weever parent population internal cells involved form takes place greatly to change in 10 days.As parent population is placed in the fresh water always, very easily dead when mating season.Breadboard way be the parent population reproduction egg-laying period near the time increase sodium chloride salinity in the water gradually to promote gonadal maturation.Concrete increase sodium chloride salinity is to increase the 0.02%-0.25%(percentage by weight every day) for good.Increase the 0.5%(percentage by weight every day) also can.Increase the 1%-2%(percentage by weight every day) then parent population is normal because of not adapting to salinity variation mortality.When the sodium chloride salinity is added to the 1%-3.2%(percentage by weight) scope in, all can hasten the hatching of silkworms and lay eggs.But as the also natural spawning that do not hasten the hatching of silkworms, only the time has prolongation slightly.When the sodium chloride salinity is in the 1.7-2.5%(percentage by weight) in the scope, spawning rate is the highest, and ovum and sperm quality are best, and fertilization rate is the highest.In order to promote the gonadal maturation of Sungkiang weever, in the stage of taking parent population gonad is accelerated the ripening, sodium chloride salt is spent in the journey in increasing water, should increase the concentration of iodine in the water simultaneously, adds sodium iodide (NaI) 5 * 10 every day
-4μ g/ml concentration adds to 0.001-0.1 μ g/ml, or adds potassium iodide (KI) 5.5371 * 10
-4μ g/ml adds to 0.002～0.2 μ g/ml, keeps this concentration then and hastens parturition.In order to satisfy other an amount of in water trace element, when increasing the sodium chloride salinity, add seawater or rough sea salt gradually, its salt content should count in the salinity that increases every day.
Three, hastening parturition of parent population: adopted fish to use luteotropin releasing hormone d-ala analog (LRH-A), fish is used human chorionic gonadtropin (HCG), four kinds of medicines of thyroxine and sodium iodide (NaI) are hastened parturition, each every tail raun injection volume is: fish is used luteotropin releasing hormone d-ala analog (LRH-A) 4.8-5.8 μ g/ tail, fish is used human chorionic gonadtropin (HCG) 2.1-3.1 unit/tail, sodium iodide (NaI) 0.001-0.003 μ g/ tail, thyroxine 4.3-6.3 μ g/ tail, milter reduces by half.General injection can be laid eggs for 1～2 time.Also can hasten parturition with human chorionic gonadtropin (HCG) with luteotropin releasing hormone d-ala analog (LRH-A) and fish as the fish of only injecting above-mentioned dosage.And for example only inject the sodium iodide (NaI) of above-mentioned dosage, also can hasten parturition.But use fish to use luteotropin releasing hormone d-ala analog (LRH-A), fish with human chorionic gonadtropin (HCG) though the mixed reagent method and the spawning rate height that uses the independent reagent method of NaI when not injecting reagent are all effective not as laying eggs of four kinds of medicine hybrid injections.
Four, fertilized egg hatching: fertilization and hatching is at the 1.7%-3.2%(percentage by weight) but equal incubation of membrane under the salinity, but normal incubation rate is the highest during with 1.9%-3% sodium chloride salinity.Incubation temperature is 4-16 ℃, the highlyest is no more than 18 ℃.The pH of hatching water is 6.2-7.2.Illumination during hatching is 400-2000Lux.The oxygen scope that contains during hatching in the water is 4-6mg/l(10-15 ℃).
Five, the raising of young seedling: young seedling is hatched and can be fed with artificial feed in back 3 days, maritime protozoa, and maritime monoplast green alga class is for the nutrition supply of yolk sac after absorbing fully got ready.After the whole absorptions of young seedling yolk sac, rely on the exogenous nutrition life fully, feed with wheel animalcule cladocera and the naupiar larva and the monoplast green alga of scratching sufficient class gradually this moment except that giving above-mentioned feed.When young seedling grows to 2cm body length, feeds such as shrimp seedling, or artificial feed can be fed gradually with hatching.
Owing to adopted the present invention in the method in five stages, make the young seedling figure of Sungkiang weever normal, nothings that afterbody is straight is crooked, and be healthy and strong, hatches then just can vertically rotatablely move in water, can get food voluntarily after a few days.The young seedling normal development rate of hatching reaches 86.0%, abnormal rate about 14.0%.It is 77% that yolk sac absorbs the phase survival rate.Hatched the back 18-24 days, yolk sac all absorbs, and also can live by getting food from the external world this moment fully.To calculating the day that the present invention is proposed patent application, young seedling has been survived 99 days, and the long 2.5-3cm of body, health have presented black decorative pattern and the figure of adult fish.The present invention provides the serial of methods of carrying out artificial propagation and cultivation in the laboratory for solving the endangered problem of Sungkiang weever, also produces Sungkiang weever for batch production practical basis is provided.
Claims (5)
1, a kind of artificial fecundation method of Sungkiang weever comprises the raising of parent population, and parent population is gonadal accelerates the ripening, the hastening parturition of parent population, and five steps of the raising of fertilized egg hatching and young seedling is characterized in that:
(1) the raising condition of parent population be pH between 6.2～7.2, illumination is controlled at 2～15 ℃ in 400～5000Lux, temperature;
(2) parent population increases sodium chloride salinity 0.02～0.25% (percentage by weight) the gonadal every day of accelerating the ripening, and adds to 1～3.2% (percentage by weight), adds sodium iodide (NaI) 5.0 * 10 simultaneously every day
-4μ g/ml, to concentration be 0.001～0.1 μ g/ml or potassium iodide (KI) 5.5 * 10
-1μ g/ml is to concentration 0.002～0.2 μ g/ml;
(3) parent population injection hormone is in order to hasten parturition;
(4) fertilized egg hatching sodium chloride salinity is at 1.7～3.2% (percentage by weights), and temperature is at 4～16 ℃, and pH is 6.2～7.2, and illumination is 400～2000Lux, and water oxygen level is 4～6mg/L;
(5) young seedling is hatched and was fed in back three days with artificial feed, add then and feed maritime protozoa, maritime monoplast green alga class, after young seedling yolk sac all absorbs, also to add the naupiar larva and the monoplast green alga of feeding wheel animalcule, cladocera and copepoda, when young seedling grows to 2cm body length, feed hatching, shrimp seedling gradually.
2, Sungkiang weever artificial fecundation method according to claim 1 is characterized in that parent population increases the sodium chloride salinity preferably to 1.7～2.5%(percentage by weight the gonadal every day of accelerating the ripening).
3, Sungkiang weever artificial fecundation method according to claim 1, it is characterized in that hastening parturition of parent population, the injection fish is used human chorionic gonadtropin (HCG) 2.1～3.1 units/tail with luteotropin releasing hormone d-ala analog (LRH-A) 4.8～5.8 μ g/ tails and fish, or injection sodium iodide (NaI) 0.001～0.003 μ g/ tail, milter reduces by half.
4, Sungkiang weever artificial fecundation method according to claim 1, hasten parturition employing LRH-A, the HCG that it is characterized in that parent population, four kinds of medicines of thyroxine and sodium iodide (NaI) mix, the per injection amount is that fish is used luteotropin releasing hormone d-ala analog (LRH-A) 4.8～5.8 μ g/ tails, fish is used human chorionic gonadtropin (HCG) 2.1～3.1 units/tail, sodium iodide (NaI) 0.001～0.003 μ g/ tail, thyroxine 4.3～6.3 μ g/ tails, milter reduces by half.
5, Sungkiang weever artificial fecundation method according to claim 1 is characterized in that the fertilized egg hatching salinity is 1.9～3%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN87101107A CN1012333B (en) | 1987-09-26 | 1987-09-26 | Artificial breeding method of trachidermus fasciatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN87101107A CN1012333B (en) | 1987-09-26 | 1987-09-26 | Artificial breeding method of trachidermus fasciatus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1031465A CN1031465A (en) | 1989-03-08 |
| CN1012333B true CN1012333B (en) | 1991-04-17 |
Family
ID=4813189
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN87101107A Expired CN1012333B (en) | 1987-09-26 | 1987-09-26 | Artificial breeding method of trachidermus fasciatus |
Country Status (1)
| Country | Link |
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| CN (1) | CN1012333B (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101138327B (en) * | 2007-10-18 | 2011-02-09 | 复旦大学 | Sungkiang weever desalination fingerling cultivating method |
| CN101411311B (en) * | 2008-12-05 | 2011-09-14 | 河北农业大学 | Production method of trachidermus fasciatus fry |
| CN104206317B (en) * | 2013-06-04 | 2016-08-24 | 江苏中洋集团股份有限公司 | The cultural method of perennial Trachi dermus Fasciatus Heckel formed by a kind of annual Trachi dermus Fasciatus Heckel |
| CN104041449B (en) * | 2014-04-04 | 2016-01-13 | 复旦大学 | The Sungkiang weever of simulate natural ecological environment breeds cultivating workshop system |
| CN107581106B (en) * | 2017-09-27 | 2018-11-30 | 海南晨海水产有限公司 | A kind of artificial fecundation method of platax orbicularis |
| CN109349171A (en) * | 2018-11-30 | 2019-02-19 | 河南省水产科学研究院 | A kind of quasi- Chang Ecology propagation method |
| CN114073232B (en) * | 2021-11-30 | 2022-11-11 | 中国水产科学研究院北戴河中心实验站 | Breeding method and application of bred parent trachidermus fasciatus |
-
1987
- 1987-09-26 CN CN87101107A patent/CN1012333B/en not_active Expired
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| Publication number | Publication date |
|---|---|
| CN1031465A (en) | 1989-03-08 |
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