CN102007874A - Artificial propagation method of pelteobagrus fulvdraco - Google Patents
Artificial propagation method of pelteobagrus fulvdraco Download PDFInfo
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- CN102007874A CN102007874A CN2009100442824A CN200910044282A CN102007874A CN 102007874 A CN102007874 A CN 102007874A CN 2009100442824 A CN2009100442824 A CN 2009100442824A CN 200910044282 A CN200910044282 A CN 200910044282A CN 102007874 A CN102007874 A CN 102007874A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention relates to an artificial propagation method of pelteobagrus fulvdraco, comprising the steps of selecting and rearing parent fish, artificially hastening parturition and hatching fish fry. The artificial hastening parturition comprises the optimized technique of parturition hastening time, setting of fish nest, parturition hastening medicine and dose and secondary injection of medicine; and the fish fry hatching comprises the design of a hatching tank, the optimized hatching technique, and the like. The method has high spawning rate, parturition hastening rate and hatching rate, can save manpower, materials and money, ensures batch production of fish fry in a planed way to meet the requirement of fish fry for different users, ensures that the production and development are unified and further avoids serious ecological damage resulting from killing a large number of male parents.
Description
Technical field
The present invention relates to the freshwater fish culturing method, further be meant the yellow cartfish artificial fecundation method.
Background technology
Yellow cartfish is under the jurisdiction of on taxonomy that Silurformes (siluriformes), fish are tasted section (Bagridae), yellow cartfish belongs to (Pseudobagrus), is commonly called as that yellow duck cries, Huang Lading, spotted maigre.Yellow cartfish is a benthic fishes, and is strong to ecotope adaptability, is distributed widely in China's fresh water water body, is a kind of important economic fish in China's rivers, the lake.Yellow cartfish is abundant because of its amino acid content, fine and tender taste, and delicious flavour, nutritious, sting between no flesh, be a kind of all-ages nutrition delicacies; This fish adapts to pond intensification high yielding culture in addition, the big lake extensive cultivation, and cage culture, and the breed proterties is good, has very vast market prospect, is described as the star of the aquatic products of 21st century.Supply falls short of demand for existing market, and price raises up again and again.Particularly wild yellow cartfish is in great shortage especially.Therefore, to yellow cartfish carry out ecology numerous support and comprehensive exploitation of far-reaching significance.
The fry of Silurformes fish such as yellow cartfish breeds, and is artificial induced spawning basically, kills hero and get essence; Annual only be used for the male parent that artificial propagation will be slaughtered up to ten million tail stalwartnesses, thereby cause in the natural waters female-male proportion seriously to be lacked of proper care, cause natural resources seriously depleted.According to the preliminary statistics, the natural amount of fishing of yellow cartfish is not as good as 10% of the sixties at present, and the amount of propagating artificially makes the possibility of yellow cartfish industrialization development almost nil less than the one thousandth of aquaculture total amount.
Moreover hero is got essence and is had a strong impact on food security extremely.Annual artificial breeding yellow cartfish need be slaughtered the male parent population of up to ten million tails, because of containing heavy dose of oxytocic drug, can produce many bad reactions after people are edible in these fishes, and health is had multiple harm.Therefore only make the yellow cartfish natural spawning could thoroughly stop the generation of this type of incident.At the bottom of there is output in existing artificial propagation yellow cartfish method, problem such as cost height, be difficult to apply.
Summary of the invention
The technical problem to be solved in the present invention is, defective at prior art exists proposes a kind of yellow cartfish artificial fecundation method, and this method can be saved human and material resources and financial resources, can in a planned way produce fry in batches to satisfy the fry demand of different user, make to produce and unify mutually with exploitation; Also can avoid causing heavy damage to natural, ecological because of killing the male parent in a large number.
Technical scheme of the present invention is that described yellow cartfish artificial fecundation method is:
(1) parent population is selected: select physique stalwartness, body surface smooth, complete, the anosis nothing of fin ray is hindered, the sexually matured individuality of profile, and the natural wild II Yellow cartfish adult in age of raun body weight 75g-100g, milter body weight 125g-150g is as the parent, and is that (be raun bar number: ratio milter bar number=1: 1.1) was put into the pond and carried out special pond and cultivate in 1: 1.1 in the quantity ratio of raun and milter;
The sexually matured parent of profile is, the raun belly is full, and is full of elasticity, upwards hold up from the back, from outside watched tangible ovary profile, the red and swollen and evagination slightly of gonopore; The milter belly is straight, and the obvious projection in gonopore position is mamillary, slightly takes on a red color;
The breeding equipment of hastening parturition can adopt four large Chinese carps (mackerel, grass carp, silver carp, bighead) the breeding facility of prior art to transform; Yellow cartfish was generally for 2 ages in the Hunan area maturation age, and annual ripe 1 time, the egg-laying period generally in the first arrival in May by the end of August.
(2) in the time of more than parent fish rearing: water temperature 12c °, manually throw something and feed with bright wild fishes or Limnodrilus hoffmeisteri, a day feeding volume is the 4%-6% of fish body weight, and parent population breeding density is that per 667 square metres of waters surface are thrown in 200kg, 1.4 meters-1.6 meters of the pond depth of waters; Also visual pond water quality and dissolved oxygen situation are regulated.
(3) artificial induced spawning:
A. hasten parturition the time: treat that the pond water temperature more than 23c ° the time, can carry out artificial induced spawning;
B. the setting of fish nest: build in the bottom of spawning pond and to build the cave, the cave diameter is 40cm-60cm, and the degree of depth is 55cm-65cm, and cave quantity is the 40%-50% of female parent population quantity, and a laying nest is placed in each cave;
Described laying nest can be organized on the iron wire circle by 50 order PEF nets and form;
C. oxytocic drug and dosage: oxytocic drug is selected luteotropin hormone analogs (LHRH-A for use
2), human chorionic gonadtropin (HCG) and DOM (DOM), injected dose is parent population per kilogram of body weight LRH-A
28-10 μ g, HCG 400IU, DOM 4-5mg, i.e. (LRH-A
28-10 μ g+HCG 400IU+DOM 4-5mg)/kg.
D. injecting method and time: adopt muscle of back injection or lumbar injection all can, depth of needle is 0.9cm-1.1cm;
Adopt the biphasic injection method: only inject raun and LH injection RH-A only for the first time
2Being injected in for the second time for the first time and only carrying out after 12 hours of injection, to raun according to dosage HCG injection, DOM, is half of raun injection volume to milter HCG injection, DOM and injection volume; Every tail parent population liquid medicine injection is 1ml;
The raun and the milter of finishing described oxytocic drug injection are put into spawning pond; The effect time is 25 hours-30 hours, 23 ℃ of-26 ℃ of the bests of the water temperature of hastening parturition;
C. get ovum: determine the parent population situation of laying eggs by the laying nest marker number, change nest twice to get ovum, in order to avoid overlong time and parent population food ovum;
(4) fry hatching: laying nest is taken out the back in clear water, wash to the greatest extent, put into the incubation tank hatching, to hatching fry; Wherein:
A. incubation tank design: be rectangular slot, the groove inwall is smooth; The colloid sparge pipe of diameter 1.5cm-2.5cm is installed at two long limit inwalls of flute length, the sparge pipe end is sealed and every 5cm-10cm the hole for water spraying that the aperture is 0.15cm-0.25cm is set on this sparge pipe, and make described hole for water spraying become 40 ° of-50 ° of angles water sprays with the same level face to the center of groove, carry out the flowing water hatching; The elevation of water spray water surface in the groove is not less than 2.5m; The incubation tank delivery port is done barrier with 80 order nylon screen windows, prevents the fry escape;
Incubation tank is generally the cement pit of long 5-8m, wide 1m, and the pond inwall makes it smooth with the ceramic tile veneer;
B. hatching management: the hatching water filters through 60 orders-80 order nylon yarn screen cloth, and water quality is pure and fresh pollution-free;
Should in time check fish-egg, find bad ovum, the ovum of not inseminating, dead ovum is artificial without exception removes; Forbid with drug disinfection in order to avoid influence incubation rate or cause medicament residue to fry.
Logical experimental study in a few years shows, uses the inventive method, and the ripe good parent population of per kilogram can lay eggs about 100,000, and the insemination rate is more than 98%, and incubation rate is more than 80%, and details see the following form 1.
Table 1:2005-2009 breeding information slip
At present the artificial propagation of Silurformes (comprising yellow cartfish, long-snout catfish, southern catfish) is all adopted and killed the male smart original method of getting; With regard to certain province, more than milter 5,000 ten thousand tails of year loss stalwartness, and the balance of a large amount of waste of manpower, material resources, financial resources and heavy damage natural, ecological, huge to the propagation influence of fish natural resources; By adopting technology such as artificial induced spawning that the present invention optimizes and natural spawning, flowing water hatching, can improve seed quantity more than 20 times, survival rate and disease-resistant rate all improve more than 60%.
Yellow cartfish artificial fecundation method of the present invention, it is the integrated practical experience of enriching of being engaged in aquatic products work decades of applicant, on basis by deep theoretical research and analysis, just establish through a large amount of and test repeatedly over a long time, it is the great achievement that fish genetic breeding aspect obtains, at nearly 1.5 hundred million tails of test site successful reproduction fry, spawning rate, spawning rate, incubation rate all maintain the leading position at home.
As known from the above, the present invention is a kind of yellow cartfish artificial fecundation method, has high spawning rate, spawning rate, incubation rate, and it can save human and material resources and financial resources, can in a planned way produce fry in batches to satisfy the fry demand of different user, make to produce and unify mutually with exploitation; Also can avoid causing heavy damage to natural, ecological because of killing the male parent in a large number.
Embodiment
Embodiment 1 yellow cartfish artificial fecundation method is:
(1) parent population is selected: select physique stalwartness, body surface smooth, complete, the anosis nothing of fin ray is hindered, the sexually matured individuality of profile, and the natural wild II Yellow cartfish adult in age of raun body weight 75g-100g, milter body weight 125g-150g is as the parent, and is that 1: 1.1 ratio is put into the pond and carried out special pond and cultivate in the quantity ratio of raun and milter;
The sexually matured parent of profile is, the raun belly is full, and is full of elasticity, upwards hold up from the back, from outside watched tangible ovary profile, the red and swollen and evagination slightly of gonopore; The milter belly is straight, and the obvious projection in gonopore position is mamillary, slightly takes on a red color;
(2) in the time of more than parent fish rearing: water temperature 12c °, manually throw something and feed with bright wild fishes or Limnodrilus hoffmeisteri, a day feeding volume is the 4%-6% of fish body weight, and parent population breeding density is that per 667 square metres of waters surface are thrown in 200kg, about 1.5 meters of the pond depth of water; Also visual pond water quality and dissolved oxygen situation are regulated.
(3) artificial induced spawning:
A. hasten parturition the time: treat that the pond water temperature more than 23c ° the time, can carry out artificial induced spawning;
B. the setting of fish nest: build in the bottom of spawning pond and to build the cave, the cave diameter is about 50cm, and the degree of depth is about 60cm, and cave quantity is the 40%-50% of female parent population quantity, and a laying nest is placed in each cave;
Described laying nest can be organized on the iron wire circle by 50 order PEF nets and form;
C. oxytocic drug and dosage: oxytocic drug is selected luteotropin hormone analogs (LHRH-A for use
2), human chorionic gonadtropin (HCG) and DOM (DOM), injected dose is parent population per kilogram of body weight LRH-A
29 μ g, HCG 400IU, DOM 4.5mg;
D. injecting method and time: adopt muscle of back injection or lumbar injection all can, depth of needle is 1cm;
Adopt the biphasic injection method: only inject raun and LH injection RH-A only for the first time
2Being injected in for the second time for the first time and only carrying out after 12 hours of injection, to raun according to dosage HCG injection, DOM, is half of raun injection volume to milter HCG injection, DOM and injection volume; Every tail parent population liquid medicine injection is 1ml;
The raun and the milter of finishing described oxytocic drug injection are put into spawning pond; The effect time is 25 hours-30 hours, 23 ℃ of-26 ℃ of the bests of the water temperature of hastening parturition;
C. get ovum: determine the parent population situation of laying eggs by the laying nest marker number, change nest twice to get ovum, in order to avoid overlong time and parent population food ovum;
(4) fry hatching: laying nest is taken out the back in clear water, wash to the greatest extent, put into the incubation tank hatching, to hatching fry; Wherein:
A. incubation tank design: be rectangular slot, the groove inwall is smooth; The colloid sparge pipe of diameter 2cm is installed at two long limit inwalls of flute length, the sparge pipe end is sealed and every 5cm-10cm the hole for water spraying that the aperture is 0.2cm is set on this sparge pipe, and described hole for water spraying is sprayed water with same level face angle at 45 to the center of groove, carry out the flowing water hatching; The elevation of water spray water surface in the groove is not less than 2.5m; The incubation tank delivery port is done barrier with 80 order nylon screen windows, prevents the fry escape;
Incubation tank is generally the cement pit of long 5-8m, wide 1m, and the pond inwall makes it smooth with the ceramic tile veneer;
B. hatching management: the hatching water filters through 60 orders-80 order nylon yarn screen cloth, and water quality is pure and fresh pollution-free;
Should in time check fish-egg, find bad ovum, the ovum of not inseminating, dead ovum is artificial without exception removes; Forbid with drug disinfection in order to avoid influence incubation rate or cause medicament residue to fry.
In the embodiment 2 yellow cartfish artificial fecundation methods, oxytocic drug is selected luteotropin hormone analogs (LHRH-A for use
2), human chorionic gonadtropin (HCG) and DOM (DOM), injected dose is parent population per kilogram of body weight LRH-A
28 μ g, HCG 400IU, DOM 4mg; All the other methods are with embodiment 1.
In the embodiment 3 yellow cartfish artificial fecundation methods, oxytocic drug is selected luteotropin hormone analogs (LHRH-A for use
2), human chorionic gonadtropin (HCG) and DOM (DOM), injected dose is parent population per kilogram of body weight LRH-A
210 μ g, HCG 400IU, DOM 5mg; All the other methods are with embodiment 1.
Claims (5)
1. a yellow cartfish artificial fecundation method is characterized in that, this method is:
(1) parent population is selected: select physique stalwartness, body surface smooth, complete, the anosis nothing of fin ray is hindered, the sexually matured individuality of profile, and the natural wild II Yellow cartfish adult in age of raun body weight 75g-100g, milter body weight 125g-150g is as the parent, and is that 1: 1.1 ratio is put into the pond and carried out special pond and cultivate in the quantity ratio of raun and milter;
(2) in the time of more than parent fish rearing: water temperature 12c °, manually throw something and feed with bright wild fishes or Limnodrilus hoffmeisteri, a day feeding volume is the 4%-6% of fish body weight, and parent population breeding density is that per 667 square metres of waters surface are thrown in 200kg, 1.4 meters-1.6 meters of the pond depth of waters;
(3) artificial induced spawning:
A. hasten parturition the time: treat that the pond water temperature more than 23c ° the time, carries out artificial induced spawning;
B. the setting of fish nest: build in the bottom of spawning pond and to build the cave, the cave diameter is 40cm-60cm, and the degree of depth is 55cm-65cm, and cave quantity is the 40%-50% of female parent population quantity, and a laying nest is placed in each cave;
C. oxytocic drug and dosage: oxytocic drug is selected luteotropin hormone analogs LHRH-A for use
2, human chorionic gonadtropin HCG and DOM DOM, injected dose is parent population per kilogram of body weight LRH-A
28-10 μ g, HCG 400IU, DOM 4-5mg;
D. injecting method and time: adopt muscle of back injection or lumbar injection all can, depth of needle is 0.9cm-1.1cm;
Adopt the biphasic injection method: only inject raun and LH injection RH-A only for the first time
2Being injected in for the second time for the first time and only carrying out after 12 hours of injection, to raun according to dosage HCG injection, DOM, is half of raun injection volume to milter HCG injection, DOM and injection volume; Every tail parent population liquid medicine injection is 1ml;
The raun and the milter of finishing described oxytocic drug injection are put into spawning pond; The effect time is 25 hours-30 hours, 23 ℃-26 ℃ of the water temperatures of hastening parturition;
C. get ovum: determine the parent population situation of laying eggs by the laying nest marker number, change nest twice to get ovum, in order to avoid overlong time and parent population food ovum;
(4) fry hatching: laying nest is taken out the back in clear water, wash to the greatest extent, put into the incubation tank hatching, to hatching fry; Wherein:
A. incubation tank design: be rectangular slot, the groove inwall is smooth; The colloid sparge pipe of diameter 1.5cm-2.5cm is installed at two long limit inwalls of flute length, the sparge pipe end is sealed and every 5cm-10cm the hole for water spraying that the aperture is 0.15cm-0.25cm is set on this sparge pipe, and make described hole for water spraying become 40 ° of-50 ° of angles water sprays with the same level face to the center of groove, carry out the flowing water hatching; The elevation of water spray water surface in the groove is not less than 2.5m; The incubation tank delivery port is done barrier with 80 order nylon screen windows, prevents the fry escape;
B. hatching management: the hatching water filters through 60 orders-80 order nylon yarn screen cloth, and water quality is pure and fresh pollution-free.
2. according to the described yellow cartfish artificial fecundation method of claim 1, it is characterized in that the sexually matured parent of profile is, the raun belly is full, and is full of elasticity, upwards hold up from the back, from outside watched tangible ovary profile, the red and swollen and evagination slightly of gonopore; The milter belly is straight, and the obvious projection in gonopore position is mamillary, slightly takes on a red color.
3. according to the described yellow cartfish artificial fecundation method of claim 1, it is characterized in that incubation tank is the cement pit of long 5-8m, wide 1m, the pond inwall makes it smooth with the ceramic tile veneer.
4. according to the described yellow cartfish artificial fecundation method of claim 1, it is characterized in that described laying nest is organized on the iron wire circle by 50 order PEF nets and forms.
5. according to the described yellow cartfish artificial fecundation method of claim 1, it is characterized in that, in the hatching process, in time check fish-egg, find bad ovum, the ovum of not inseminating, dead ovum is artificial without exception removes.
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103004663A (en) * | 2012-12-21 | 2013-04-03 | 苏州市水产养殖场有限公司 | Pelteobagrus fulvidraco artificial propagation process |
| CN103461250A (en) * | 2013-09-27 | 2013-12-25 | 苏州市阳澄湖现代农业发展有限公司 | Method for culturing pelteobagrus fulvidraco in rice field |
| CN103583431A (en) * | 2013-11-10 | 2014-02-19 | 华中农业大学 | Method for mainly culturing yellow catfishes and carrying out polyculture on other feeding fishes in pond |
| CN104904637A (en) * | 2015-06-11 | 2015-09-16 | 刘有文 | Method of cultivating fry of Pelteobagrus fulvidraco |
| CN104938385A (en) * | 2015-06-23 | 2015-09-30 | 周天赐 | Hemibarbus maculatus bleeker artificial reproduction method |
| CN105409851A (en) * | 2015-12-15 | 2016-03-23 | 黄伟 | Feeding method for yellow catfishes |
| CN104705232B (en) * | 2015-04-10 | 2017-01-11 | 宁国市金东坊农业开发有限公司 | Ecology-simulated artificial tor (foliter) brevifilis brevifilis breeding method by virtue of earthen pond |
| CN106804482A (en) * | 2016-12-21 | 2017-06-09 | 湖州市水产技术推广站 | A kind of artificial fecundation method of autumn Pelteobagrus fulvidraco seedling |
| CN106804477A (en) * | 2015-11-30 | 2017-06-09 | 宁陕县绿康农业生态科技有限公司 | A kind of artificial fecundation method of Pelteobagrus fulvidraco |
| CN107278981A (en) * | 2017-07-07 | 2017-10-24 | 佛山市蓝科养殖科技有限公司 | A kind of mating system for hybridizing yellow bone fish |
| CN107736280A (en) * | 2017-11-29 | 2018-02-27 | 安徽金阳光农业服务有限公司 | A kind of Pelteobagrus fulvidraco parent population reinforced cultivating and biological control technology |
| CN109220910A (en) * | 2018-11-23 | 2019-01-18 | 安岳县佳兴水产养殖专业合作社 | A kind of breeding method for increasing of white snakeheaded fish |
| CN112335582A (en) * | 2020-11-05 | 2021-02-09 | 安徽省农业科学院水产研究所 | Artificial breeding method for hybrid pelteobagrus fulvidraco |
| CN113925958A (en) * | 2021-09-30 | 2022-01-14 | 南方海洋科学与工程广东省实验室(湛江) | Oxytocic for Seriola quinqueradiata and oxytocic method |
| CN114868676A (en) * | 2022-03-08 | 2022-08-09 | 西双版纳云博水产养殖开发有限公司 | Spawning induction method suitable for male meinan silurus meridionalis |
-
2009
- 2009-09-07 CN CN2009100442824A patent/CN102007874A/en active Pending
Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103004663A (en) * | 2012-12-21 | 2013-04-03 | 苏州市水产养殖场有限公司 | Pelteobagrus fulvidraco artificial propagation process |
| CN103461250A (en) * | 2013-09-27 | 2013-12-25 | 苏州市阳澄湖现代农业发展有限公司 | Method for culturing pelteobagrus fulvidraco in rice field |
| CN103461250B (en) * | 2013-09-27 | 2015-07-29 | 苏州市阳澄湖现代农业发展有限公司 | A kind of method of paddy field aquaculture yellow cartfish |
| CN103583431A (en) * | 2013-11-10 | 2014-02-19 | 华中农业大学 | Method for mainly culturing yellow catfishes and carrying out polyculture on other feeding fishes in pond |
| CN104705232B (en) * | 2015-04-10 | 2017-01-11 | 宁国市金东坊农业开发有限公司 | Ecology-simulated artificial tor (foliter) brevifilis brevifilis breeding method by virtue of earthen pond |
| CN104904637A (en) * | 2015-06-11 | 2015-09-16 | 刘有文 | Method of cultivating fry of Pelteobagrus fulvidraco |
| CN104938385A (en) * | 2015-06-23 | 2015-09-30 | 周天赐 | Hemibarbus maculatus bleeker artificial reproduction method |
| CN106804477A (en) * | 2015-11-30 | 2017-06-09 | 宁陕县绿康农业生态科技有限公司 | A kind of artificial fecundation method of Pelteobagrus fulvidraco |
| CN105409851A (en) * | 2015-12-15 | 2016-03-23 | 黄伟 | Feeding method for yellow catfishes |
| CN106804482A (en) * | 2016-12-21 | 2017-06-09 | 湖州市水产技术推广站 | A kind of artificial fecundation method of autumn Pelteobagrus fulvidraco seedling |
| CN107278981A (en) * | 2017-07-07 | 2017-10-24 | 佛山市蓝科养殖科技有限公司 | A kind of mating system for hybridizing yellow bone fish |
| CN107736280A (en) * | 2017-11-29 | 2018-02-27 | 安徽金阳光农业服务有限公司 | A kind of Pelteobagrus fulvidraco parent population reinforced cultivating and biological control technology |
| CN109220910A (en) * | 2018-11-23 | 2019-01-18 | 安岳县佳兴水产养殖专业合作社 | A kind of breeding method for increasing of white snakeheaded fish |
| CN112335582A (en) * | 2020-11-05 | 2021-02-09 | 安徽省农业科学院水产研究所 | Artificial breeding method for hybrid pelteobagrus fulvidraco |
| CN113925958A (en) * | 2021-09-30 | 2022-01-14 | 南方海洋科学与工程广东省实验室(湛江) | Oxytocic for Seriola quinqueradiata and oxytocic method |
| CN114868676A (en) * | 2022-03-08 | 2022-08-09 | 西双版纳云博水产养殖开发有限公司 | Spawning induction method suitable for male meinan silurus meridionalis |
| CN114868676B (en) * | 2022-03-08 | 2022-11-11 | 西双版纳云博水产养殖开发有限公司 | Spawning induction method suitable for male meinan silurus meridionalis |
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Application publication date: 20110413 |