CN104082213A - Artificial propagation method for Taiwan mud fishes - Google Patents
Artificial propagation method for Taiwan mud fishes Download PDFInfo
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- CN104082213A CN104082213A CN201410333609.0A CN201410333609A CN104082213A CN 104082213 A CN104082213 A CN 104082213A CN 201410333609 A CN201410333609 A CN 201410333609A CN 104082213 A CN104082213 A CN 104082213A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses an artificial propagation method for Taiwan mud fishes and belongs to the propagation technologies for fresh water mud fishes. The method includes the following steps that (1), parent mud fish fries are selected; (2), artificial spawning induction is performed; (3), artificial fertilization is performed; (4), hatching is performed. According to the technology, the method that human chorionic gonadotropin (HCG), hypophyses or luteotropin releasing hormone d-ala analogs and other spawning induction medicine are injected to the backs of the parent mud fishes is adopted, so that the spawning induction rate of the parent mud fishes is increased over 91.0%; according to the technology, the fertilization rate of the parent mud fishes is increased over 96.6% through artificial fertilization, and the hatching rate of the parent mud fishes is increased over 98.0% through still water hatching; the survival rate of the parent mud fishes after artificial propagation reaches 90.9%. Propagation is performed by the utilization of the method, so that the survival rate, the spawning induction rate, the fertilization rate and the hatching rate of the Taiwan mud fishes are much higher than the corresponding rates through natural propagation and traditional propagation method for the mud fishes, industrialized production of the Taiwan mud fishes can be achieved, the fries are provided for large-scale farming, and high economical benefits are obtained.
Description
Technical field
The invention belongs to fresh water bullhead propagation technique, particularly relate to a kind of loach artificial fecundation method.
Background technology
Chinese snake head is the small-sized fresh water bullhead in Cypriniformes, due to its fine and tender taste, light, delicious, and has nourishing medicinal function, so always for people like food.Chinese snake head is nutritious, contains rich in protein in its meat, fat, vitamin, calcium, iodine, iron etc., and there is higher unsaturated fatty acid.
Because the market demand increases, agricultural chemicals uses in a large number and catching intensity increase and the rise of the special aquatic products using Chinese snake head as feed, cause Chinese snake head natural resources far can not meet people's needs.Chinese snake head vitality is stronger, be suitable for carrying out propagating artificially, but current China does not also form the cultural technique system of a set of maturation.Also exist problems to cause the injured or dead ratio of loach up to 50% at close loach cultivation, the aspect such as mode, incubating oosperm of hastening parturition.The breeding problem of Chinese snake head has become the bottleneck of restriction Chinese snake head industry development.
Summary of the invention
The present invention is directed to Chinese snake head breeding the deficiencies in the prior art, provide that a kind of method is reasonable, workable, the artificial propagation new method of the Chinese snake head seed that is produced on a large scale.The present invention, by optimizing the technology such as close loach cultivation, artificial induced spawning, fertilization and hatching, particularly uses formula and the dosage of three kinds of different oxytocic drugs, thereby has reduced the lethality of breeding close loach, has improved spawning rate, fertilization rate, incubation rate.
To achieve these goals, the technical solution adopted in the present invention is as follows:
An artificial fecundation method for Chinese snake head, the method comprises the following steps:
(1) close loach seed selection
Select the close loach in above contiguous egg-laying period in 1 age half, female loach requires belly full, soft, and gonopore mastoid process is given prominence to greatly, and hole circle becomes blush, long 25～30 centimetres of body, body weight is more than 100 grams, and male loach requires body colour bright-coloured, have obvious nuptial coloration, long 25～30 centimetres of body, more than 120 grams;
(2) artificial induced spawning
Constant water temperature is at 20～26 degree, and requiring male and female parent loach is the ratio of 1:2 or 1:3, to putting into net cage after close loach injection oxytocic drug; Described oxytocic drug is human chorionic gonadtropin (HCG), hypophysis or luteotropin releasing hormone d-ala analog (LRH～A);
(3) artificial insemination
After hastening parturition, after 8～15 hours, first the seminal fluid of male loach is extruded, then ovum is expressed in vessel, sperm is watered on ovum, stir gently and make it to mix, stirred for several adds a small amount of clear water to strengthen sperm viability after second, improve fertilization rate; Obtain rinsing with clear water after fertilized egg;
(4) hatching
Fertilized egg after rinsing is put into hatching pail, Lentic hatching, Spawning water requires pure and fresh, water nonpollution, dissolved oxygen content at 6mg/L～7mg/L, pH between 7～8, and every 1mL water body is put 2～3, fertilized egg in a suitable place to breed.Suitable water temperature when hatching is 18 DEG C～28 DEG C, within 30 hours～35 hours, can hatch young loach.
Wherein, oxytocic drug injection volume is every gram of body weight injection 20～40 international unit HCG of female loach, every gram of body weight injection 10～20 international unit HCG of male loach;
Wherein, oxytocic drug injection volume is female spade injection 300～400 international unit HCG, or 1 of carp loach hypophysis, or LRH～A approximately 5 μ g～10 μ g, and male loach injection volume reduces by half;
Wherein, oxytocic drug injection volume is 2 of the hypophysis that female loach injecting body focuses on 0.5 kilogram of left and right carp loach, and male loach injection volume reduces by half.
Wherein, described injecting method is the shot of back of the body eel base portion muscle, and when injection, syringe and axon are 30 ° of angles, by backward front insertion.
Beneficial effect of the present invention:
This technology can significantly improve spawning rate, fertilization rate, incubation rate and close loach survival rate in postpartum.Utilize this artificial fecundation method can realize the seedling industrialized production of Chinese snake head, for large-scale farming provides seed, obtain good economic benefit.Be 90.9% by this artificial fecundation method parent in postpartum loach survival rate, spawning rate is 91.0%, and fertilization rate is 96.6%, and incubation rate is 98.0%, and above-mentioned ratio is far away higher than the ratio of loach natural propagation and conventional method breeding.
Embodiment
The present invention is described further below, and the present embodiment only represents a kind of preferred forms, should not be construed as limiting the invention.
Embodiment mono-:
An artificial fecundation method for Chinese snake head, the method comprises the following steps:
Select above close loach in 1 age half, female loach 100 tails, belly is full, soft, and gonopore mastoid process is given prominence to greatly, and hole circle becomes blush, long 25～30 centimetres of body, body weight is more than 100 grams; Male loach 120 tails, body colour is bright-coloured, has obvious nuptial coloration, long 25～30 centimetres of body, body weight is more than 120 grams.Constant water temperature is at 20 DEG C, and male and female parent loach is 1:2 ratio, and to female loach injection injection 300 international unit HCG, male loach is injected 150 international unit HCG, hastens parturition, and after hastening parturition, puts into net cage.Hasten parturition after 8 hours, first the seminal fluid of male loach is extruded, then ovum is expressed in vessel, sperm is watered to ovum, stir gently and make it to mix, stirred for several adds a small amount of clear water after second, obtain fertilized egg.Rinse fertilized egg 3 times with clear water, after flushing, fertilized egg is put into hatching pail, Lentic hatching, the dissolved oxygen content of Spawning water is 7 at 6mg/L, pH, every 1mL water body is put 2, fertilized egg in a suitable place to breed.Suitable water temperature when hatching is 18 DEG C, after 30 hours, hatches young loach.
From hatching pail, select 1000 of 3 point samplings, check that feritilization of ovum rate is respectively 95%, 98%, 97%, average out to 96.6%.Check, record the Ci Qiu mantissa of laying eggs, calculating spawning rate is 91.0%.Postpartum parent loach is transferred in pond, recorded the number of surviving in 15 days, result is: female loach 91 tails, and male loach 109 tails, the close loach survival rate of hastening parturition is 90.9%.
Embodiment bis-:
An artificial fecundation method for Chinese snake head, the method comprises the following steps:
Select above close loach in 1 age half, female loach 100 tails, belly is full, soft, and gonopore mastoid process is given prominence to greatly, and hole circle becomes blush, long 25～30 centimetres of body, body weight is more than 100 grams; Male loach 130 tails, body colour is bright-coloured, has obvious nuptial coloration, long 25～30 centimetres of body, body weight is more than 120 grams.Constant water temperature is at 26 DEG C, and male and female parent loach is 1:2 ratio, and to female loach injection injection 300 international unit HCG, male loach is injected 150 international unit HCG, hastens parturition, and after hastening parturition, puts into net cage.Hasten parturition after 15 hours, first the seminal fluid of male loach is extruded, then ovum is expressed in vessel, sperm is watered to ovum, stir gently and make it to mix, stirred for several adds a small amount of clear water after second, obtain fertilized egg.Rinse fertilized egg 5 times with clear water, after flushing, fertilized egg is put into hatching pail, Lentic hatching, the dissolved oxygen content of Spawning water is 8 at 7mg/L, pH, every 1mL water body is put 3, fertilized egg in a suitable place to breed.Suitable water temperature when hatching is 28 DEG C, after 35 hours, hatches young loach.
In hatching pail, select 1000 of 3 point samplings, check that feritilization of ovum rate is respectively 97%, 96%, 97%, average out to 96.6%.Check, record the Ci Qiu mantissa of laying eggs, calculating spawning rate is 91.8%.Postpartum parent loach is transferred in pond, recorded the number of surviving in 15 days, result is: female loach 89 tails, and male loach 124 tails, the close loach survival rate of hastening parturition is 92.6%.
Claims (4)
1. an artificial fecundation method for Chinese snake head, the method comprises the following steps:
(1) close loach seed selection
Select the close loach in above contiguous egg-laying period in 1 age half, female loach requires belly full, soft, and gonopore mastoid process is given prominence to greatly, and hole circle becomes blush, long 25～30 centimetres of body, body weight is more than 100 grams, and male loach requires body colour bright-coloured, have obvious nuptial coloration, long 25～30 centimetres of body, more than 120 grams;
Artificial induced spawning
Constant water temperature is at 20～26 degree, and requiring male and female parent loach is the ratio of 1:2 or 1:3, to putting into net cage after close loach injection oxytocic drug; Described oxytocic drug is human chorionic gonadtropin (HCG), hypophysis or luteotropin releasing hormone d-ala analog (LRH～A);
Artificial insemination
After hastening parturition, after 8～15 hours, first the seminal fluid of male loach is extruded, then ovum is expressed in vessel, sperm is watered on ovum, stir gently and make it to mix, stirred for several adds a small amount of clear water to strengthen sperm viability after second, improve fertilization rate; Obtain rinsing with clear water after fertilized egg;
Hatching
Fertilized egg after rinsing is put into hatching pail, Lentic hatching, Spawning water requires pure and fresh, water nonpollution, dissolved oxygen content at 6mg/L～7mg/L, pH between 7～8, and every 1mL water body is put 2～3, fertilized egg in a suitable place to breed.
2. the suitable water temperature while hatching is 18 DEG C～28 DEG C, within 30 hours～35 hours, can hatch young loach.
3. the artificial fecundation method of a kind of Chinese snake head as claimed in claim 1, is characterized in that: described oxytocic drug injection volume is every gram of body weight injection 20～40 international unit HCG of female loach, every gram of body weight injection 10～20 international unit HCG of male loach;
The artificial fecundation method of a kind of Chinese snake head as claimed in claim 1, it is characterized in that: described oxytocic drug injection volume is female spade injection 300～400 international unit HCG, or 1 of carp loach hypophysis, or LRH～A is 5 μ g～10 μ g, male loach injection volume reduces by half;
The artificial fecundation method of a kind of Chinese snake head as claimed in claim 1, is characterized in that: described oxytocic drug injection volume is 2 of the hypophysis that female loach injecting body focuses on 0.5 kilogram of left and right carp loach, and male loach injection volume reduces by half.
4. the artificial fecundation method of a kind of Chinese snake head as claimed in claim 1, is characterized in that: described injecting method is the shot of back of the body eel base portion muscle, and when injection, syringe and axon are 30 ° of angles, by backward front insertion.
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| CN201410333609.0A CN104082213A (en) | 2014-07-15 | 2014-07-15 | Artificial propagation method for Taiwan mud fishes |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104381168A (en) * | 2014-11-04 | 2015-03-04 | 广安市前锋区华瑞泥鳅养殖有限责任公司 | Rapid propagation method for mud fish |
| CN104542398A (en) * | 2015-01-04 | 2015-04-29 | 铜陵县博陵农业生态园 | Spring and autumn two-season propagation method for loaches |
| CN105532520A (en) * | 2015-12-07 | 2016-05-04 | 内江师范学院 | Sinibotiareevesa seedling breeding method |
| CN105557563A (en) * | 2014-10-10 | 2016-05-11 | 龙门县新瑶种养专业合作社 | Taiwan loach high-efficiency industrialized breeding method |
| CN105746399A (en) * | 2016-03-07 | 2016-07-13 | 五河县金满塘生态种养殖家庭农场 | Crucian breeding quality improvement method |
| CN106550910A (en) * | 2016-11-18 | 2017-04-05 | 黄郴 | A kind of Misgurni anguillicaudati artificial breeding method |
| CN107711611A (en) * | 2017-10-17 | 2018-02-23 | 铜仁职业技术学院 | A kind of low damage of Chinese snake head, high-efficiency artificial propagation method |
| CN108308071A (en) * | 2018-01-10 | 2018-07-24 | 广西平南县平原农牧有限公司 | A kind of efficient industrialized propagation method of Chinese snake head |
| CN108719146A (en) * | 2018-05-04 | 2018-11-02 | 铜仁市万山区水产站 | A kind of loach breeding method |
| CN108739541A (en) * | 2018-06-14 | 2018-11-06 | 吴林 | A kind of Misgurnus auguillicaudatus autumn and winter propagation method |
| CN108739533A (en) * | 2018-05-04 | 2018-11-06 | 铜仁市万山区水产站 | A kind of loach breeding method |
| CN109122419A (en) * | 2018-09-12 | 2019-01-04 | 河南昌佳农业发展有限公司 | A kind of method of loach crossbreed |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105557563A (en) * | 2014-10-10 | 2016-05-11 | 龙门县新瑶种养专业合作社 | Taiwan loach high-efficiency industrialized breeding method |
| CN104381168A (en) * | 2014-11-04 | 2015-03-04 | 广安市前锋区华瑞泥鳅养殖有限责任公司 | Rapid propagation method for mud fish |
| CN104542398A (en) * | 2015-01-04 | 2015-04-29 | 铜陵县博陵农业生态园 | Spring and autumn two-season propagation method for loaches |
| CN105532520B (en) * | 2015-12-07 | 2019-06-04 | 内江师范学院 | A kind of expanded letter sand loach seedlings kind propagation method |
| CN105532520A (en) * | 2015-12-07 | 2016-05-04 | 内江师范学院 | Sinibotiareevesa seedling breeding method |
| CN105746399A (en) * | 2016-03-07 | 2016-07-13 | 五河县金满塘生态种养殖家庭农场 | Crucian breeding quality improvement method |
| CN106550910A (en) * | 2016-11-18 | 2017-04-05 | 黄郴 | A kind of Misgurni anguillicaudati artificial breeding method |
| CN107711611A (en) * | 2017-10-17 | 2018-02-23 | 铜仁职业技术学院 | A kind of low damage of Chinese snake head, high-efficiency artificial propagation method |
| CN108308071A (en) * | 2018-01-10 | 2018-07-24 | 广西平南县平原农牧有限公司 | A kind of efficient industrialized propagation method of Chinese snake head |
| CN108719146A (en) * | 2018-05-04 | 2018-11-02 | 铜仁市万山区水产站 | A kind of loach breeding method |
| CN108739533A (en) * | 2018-05-04 | 2018-11-06 | 铜仁市万山区水产站 | A kind of loach breeding method |
| CN108739541A (en) * | 2018-06-14 | 2018-11-06 | 吴林 | A kind of Misgurnus auguillicaudatus autumn and winter propagation method |
| CN109122419A (en) * | 2018-09-12 | 2019-01-04 | 河南昌佳农业发展有限公司 | A kind of method of loach crossbreed |
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Application publication date: 20141008 |