CN109122419A - A kind of method of loach crossbreed - Google Patents
A kind of method of loach crossbreed Download PDFInfo
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- CN109122419A CN109122419A CN201811060278.2A CN201811060278A CN109122419A CN 109122419 A CN109122419 A CN 109122419A CN 201811060278 A CN201811060278 A CN 201811060278A CN 109122419 A CN109122419 A CN 109122419A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses a kind of method of loach crossbreed, the method for the loach crossbreed, comprising the following steps: Selection parent loach: select that Chinese snake head is male loach, green loach is female loach；Chinese snake head and green loach are respectively put into the storage pond, holding pond sterilized and are cultivated；It hastens parturition；Using HCG, LRH-A3With the parent loach in DOM mixing oxytocin injections agent injection storage pond, holding pond；Using it is artificial squeeze ovum by the way of, the ovum for injecting the green loach of oxytocins is got into hybridization pond, then the sperm for injecting the Chinese snake head of oxytocins is got into hybridization pond again, and stir evenly and make its fertilization, obtains fertilized eggs；Fertilized eggs are put into incubator, hybridization loach seedling is hatched to obtain.The invention has the benefit that it for male loach, blue mud loach is female loach that a kind of loach cross breeding method of the present invention, which uses Chinese snake head, obtained hybridization loach growing way is fast, delicious flavour, and adaptability is stronger, it is suitble to large-scale farming, economic benefits comparison is high.
Description
Technical field
The present invention relates to a kind of cultural methods of aquatic products, and in particular to a kind of method of loach crossbreed.
Background technique
Chinese snake head is one kind of big squama pair loach, and the shoal of the middle and lower reach of Yangtze River and the island of Taiwan northwestward is distributed in China more
River, mostly based on water surface life style, growing way is compared with strong, growth fraction is very fast, but its meat is general, because it is with water surface life
It is main, it is subject to birds and the harm of other natural enemies.
Green loach is a kind of very high fish of nutritive value, lives in the static or slow flow water bodies at mud bottom, often haunts lake
The mud surface layer of pool, pond, irrigation canals and ditches and paddy field bottom rich in canebreak, adaptability is stronger, can be very abundant containing humus
Life in environment, life water temperature are 10-30 DEG C, and optimum water temperature is 25-27 DEG C, and when water temperature is increased to 30 DEG C, loach is slipped into
It aestivates in mud, when water temperature drop is to 5 DEG C or less, that is, it is overwintering to pierce the depths 20-30cm in mud.When water anoxic, intestines can be carried out
Breathing, and after water body is dry, and can pierce in mud and hide, it is food with various meiofaunas, meat is excellent for outlet aquatic products
One of product.
Loach has wide domestic and international market, and at home, loach is considered as nourishing and fit keeping function all the time by Chinese people
Good merchantable brand, the market demand rises every year, and the market price is steadily increasing, and the price of loach is even higher than common eel, also often for not answering
It asks.Therefore, many departments and when the scholarly forecast coming years most promising aquatic products, all using loach breeding as popular object
One of.
The growing way of Chinese snake head is very fast compared with strong, growth fraction but its meat is general, not as good as the soft mouth of feeling well of green loach, and Taiwan
Loach is subject to birds and the harm of other natural enemies, survival rate is relatively low based on being lived with the water surface；Green loach meat relatively feel well it is tender,
It is good in taste, and adaptability is stronger, and growing way does not have Chinese snake head growing way fast, but green loach is mostly to live in underwater sludge
It is main, cause its captures more troublesome, therefore Chinese snake head is hybridized with blueness loach, cultivate a kind of growing way it is good, it is in good taste, be easy to catch
A kind of hybridization loach preyed on by bird is caught and be not easy, is a kind of demand in present market.
Summary of the invention
A kind of breeding for the hybridization loach that the object of the present invention is to provide growing ways is good, in good taste, survival rate is high, is easy to capture
Method.
A kind of method of loach crossbreed, comprising the following steps:
(1) Selection parent loach: select that Chinese snake head is male loach, green loach is female loach；
(2) Chinese snake head and green loach are respectively put into the storage pond, holding pond sterilized and are cultivated；
(3) it hastens parturition: using HCG, LRH-A3With the parent loach in DOM mixing oxytocin injections agent injection storage pond, holding pond；
(4) by the way of artificial crowded ovum, the ovum for injecting the green loach of oxytocins is got into hybridization pond, it then again will note
The sperm for penetrating the Chinese snake head of oxytocins is got into hybridization pond, and is stirred evenly and made its fertilization, and fertilized eggs are obtained；
(5) fertilized eggs are put into incubator, hatch to obtain hybridization loach seedling.
Based on Chinese snake head is mostly lived with the water surface, the comparison of the growth is fast but meat is bad, is easy during the growth process by bird
Class and the harm of other natural enemies, survival rate is relatively low, but it has the advantages that growing way is fast；Green loach mostly based on the life of mud bottom, is fitted
Answering property is stronger, and growing way is slow, delicious meat, because it is movable mostly in mud bottom, causes to capture difficult；By Chinese snake head and blueness
Loach hybridization, it is faster than green loach to obtain growing way, and based on living below the water surface, adaptability is relatively strong, delicious meat hybridization loach,
It overcomes Chinese snake head and is easy the drawbacks of being attacked by birds, also overcoming green loach, life is not easy the drawbacks of capturing at mud bottom；And
Growing way is fast, and delicious flavour, adaptability is stronger, is suitble to large-scale farming, and economic benefits comparison is high.
In addition, the suitable growth temperature of Chinese snake head is 25-32 DEG C, growth temperature is higher, and the growth temperature of green loach is 10-
30 DEG C, Chinese snake head is hybridized with green loach, the loach Adaptable growth temperature after hybridization is wider, i.e., adaptability compares
By force.
Chinese snake head and green loach are respectively put into the storage pond, holding pond sterilized and are cultivated, the storage pond, holding pond selected herein can
Think glass jar.
Further, it in step (2), is carried out disinfection with quick lime to storage pond, holding pond, after 3-5 days to be sterilized, then toward storage pond, holding pond
Middle injection water, and Chinese snake head and green loach are respectively put into storage pond, holding pond and are cultivated.Chinese snake head and green loach are hybridized
Before, it is individually placed to be fed in storage pond, holding pond, it is therefore an objective to make Chinese snake head and green loach adapt to the environment that need to hybridize, be conducive to
It is ovulated successfully.
Further, in step (3), the dosage of every kilogram of Chinese snake head HCG injection is 160-170 international unit；Injection
LRH-A3Dosage be 4.8-5mg；The dosage for injecting DOM is 0.78-0.85mg；The injection dosage of green loach halves.To parent's mud
Loach is raised in storage pond, holding pond, and after the environment before having adapted to hybridize, parent's loach injection oxytocins is hastened parturition；Certainly injection
The time of oxytocins will be selected within the scope of the normal ovulation period of Chinese snake head and green loach.
Further, it in step (4), after Chinese snake head and blue mud loach oxytocin injection 6-9h, then carries out manually squeezing ovum.
Further, it in step (4), is squeezed after the ovum of green loach gets into hybridization pond 8-10min, then by the sperm of Chinese snake head
Into hybridization pond.
Further, Chinese snake head and the quantity ratio of green loach are 1:10-12.
Further, in step (4), after the sperm of the ovum of green loach and Chinese snake head gets into hybridization pond 5-10min, then
Stirring evenly makes its fertilization.It being linked togather because the ovum that the mode of artificial crowded ovum is extruded glues, sperm is viscous to be linked togather, and to blueness
After the ovum of loach and the sperm of Chinese snake head get into hybridization pond, sperm is made to contact and be fertilized with ovum.
Further, in step (4), the speed of the stirring is 20-30r/min.It stirs to make viscous be linked togather
Fertilized eggs are separated from each other, and can make to be sufficiently mixed between unfertilized ovum and sperm.It has to protect when stirring
It holds and mixes slowly, avoid damage to the fertilized eggs generated.
Further, in step (4), after stopping 30-40min to be mixed, fertilized eggs is rinsed with clear water, make to be bonded together
Fertilized eggs separate to form individual, after stopping 30-40min to be mixed, fertilized eggs are rinsed with clear water, in order to make viscous be linked togather
Fertilized eggs separate, and form single individual, convenient for hatching.
Further, the incubation temperature of the incubator be 23-28 DEG C, brooding time 60-72h.When hatching, it will hybridize
Pond moves directly to be hatched in incubator.
The invention has the benefit that a kind of loach cross breeding method of the present invention uses Chinese snake head for male mud
Loach, blue mud loach are female loach, and Chinese snake head is hybridized with green loach, and it is faster than green loach to obtain growing way, are with life below the water surface
Main, adaptability is relatively strong, delicious meat hybridization loach, and which overcome Chinese snake heads to be easy the drawbacks of being attacked by birds, also overcomes
Green loach is not easy the drawbacks of capturing in the life of mud bottom；And growing way is fast, and delicious flavour, adaptability is stronger, it is suitble to large-scale farming,
Economic benefits comparison is high.
In addition, a kind of loach cross breeding method of the present invention, Technical comparing is simple, and Chinese snake head and green loach are carried out
Manually crowded ovum, is then again hatched fertilized eggs, Technical comparing is simple, and the higher suitable people of survival rate support on a large scale
It grows.
Specific embodiment
Embodiment one
A kind of method of loach crossbreed, comprising the following steps:
(1) Selection parent loach: select that Chinese snake head is male loach, green loach is female loach；
(2) Chinese snake head and green loach are respectively put into the storage pond, holding pond sterilized and are cultivated；
(3) it hastens parturition: using HCG, LRH-A3With the parent loach in DOM mixing oxytocin injections agent injection storage pond, holding pond；
(4) by the way of artificial crowded ovum, the ovum for injecting the green loach of oxytocins is got into hybridization pond, it then again will note
The sperm for penetrating the Chinese snake head of oxytocins is got into hybridization pond, and is stirred evenly and made its fertilization, and fertilized eggs are obtained；
(5) fertilized eggs are put into incubator, hatch to obtain hybridization loach seedling.
Embodiment two
A kind of method of loach crossbreed, comprising the following steps:
Selection parent loach: select that Chinese snake head is male loach, green loach is female loach；Chinese snake head is mostly lived with the water surface
Based on, the comparison of the growth is fast but meat is bad, is easy to be endangered by birds and other natural enemies during the growth process, survival rate compares
It is low, but it has the advantages that growing way is fast；Green loach is mostly based on the life of mud bottom, and adaptability is stronger, and growing way is slow, and meat is fresh
Beauty causes to capture difficult because it is movable mostly in mud bottom；Chinese snake head is hybridized with green loach, it is faster than green loach to obtain growing way,
Based on living below the water surface, adaptability is relatively strong, delicious meat hybridization loach, is easy to be attacked by birds which overcome Chinese snake head
The drawbacks of hitting, also overcoming green loach, life is not easy the drawbacks of capturing at mud bottom；And growing way is fast, delicious flavour, adaptability is stronger,
It is suitble to large-scale farming, economic benefits comparison is high.
Chinese snake head and green loach are respectively put into the glass jar storage pond, holding pond sterilized and are cultivated, glass jar storage pond, holding pond makes
It is carried out disinfection with quick lime, and after disinfection 3 days, then injects water into storage pond, holding pond, be then respectively put into Chinese snake head and green loach
It is cultivated in storage pond, holding pond, to adapt it to the environment before hybridization, convenient for its oviposition；After being cultivated 2 days in storage pond, holding pond, respectively
Hasten parturition processing, i.e. oxytocin injection are carried out to Chinese snake head and green loach, the amount of oxytocin injection is every kilogram of Chinese snake head injection
The dosage of HCG is 160 international units；Inject LRH-A3Dosage be 4.8mg；The dosage for injecting DOM is 0.78mg；The note of green loach
Dosage is penetrated to halve；The time of certain oxytocin injection will be selected within the scope of the normal ovulation period of Chinese snake head and green loach；And
The Chinese snake head of oxytocin injection and the quantity ratio of green loach are 1:10.
After Chinese snake head and blue mud loach oxytocin injection 6h, i.e., its this when being ovulated, using it is artificial squeeze ovum by the way of,
The ovum for injecting the green loach of oxytocins is got into hybridization pond, was urged after the ovum of green loach gets into hybridization pond 8min, then by injecting
The sperm for producing the Chinese snake head of element is got into hybridization pond；Hybridization pond 5min is got into the ovum of green loach and the sperm of Chinese snake head
Afterwards, it is stirred for uniformly making its fertilization；Speed when stirring will maintain 20r/min slowly under normal circumstances, prevent mixing speed mistake
Fast to destroy fertilized eggs, mixing slowly can be such that the viscous fertilized eggs being linked togather separate, and can make sperm and ovum more fully
It contacts, after stopping 30min to be mixed, rinses fertilized eggs with clear water, make the viscous fertilized eggs being linked togather separate to form individual, be convenient for
Hatching；
Fertilized eggs are put into incubator and are hatched, fertilization pond can be directly placed into incubator carry out for convenience
Hatching, at 23 DEG C, the time is controlled in 60h for the temperature control of hatching；After hatching, loach seedling, which is carried out normal feed, is
It can.
Embodiment three
A kind of method of loach crossbreed, comprising the following steps:
Selection parent loach: select that Chinese snake head is male loach, green loach is female loach；Chinese snake head is mostly lived with the water surface
Based on, the comparison of the growth is fast but meat is bad, is easy to be endangered by birds and other natural enemies during the growth process, survival rate compares
It is low, but it has the advantages that growing way is fast；Green loach is mostly based on the life of mud bottom, and adaptability is stronger, and growing way is slow, and meat is fresh
Beauty causes to capture difficult because it is movable mostly in mud bottom；Chinese snake head is hybridized with green loach, it is faster than green loach to obtain growing way,
Based on living below the water surface, adaptability is relatively strong, delicious meat hybridization loach, is easy to be attacked by birds which overcome Chinese snake head
The drawbacks of hitting, also overcoming green loach, life is not easy the drawbacks of capturing at mud bottom；And growing way is fast, delicious flavour, adaptability is stronger,
It is suitble to large-scale farming, economic benefits comparison is high
Chinese snake head and green loach are respectively put into the glass jar storage pond, holding pond sterilized and are cultivated, glass jar storage pond, holding pond makes
It is carried out disinfection with quick lime, and after disinfection 4 days, then injects water into storage pond, holding pond, be then respectively put into Chinese snake head and green loach
It is cultivated in storage pond, holding pond, to adapt it to the environment before hybridization, convenient for its oviposition；After being cultivated 2 days in storage pond, holding pond, respectively
Hasten parturition processing, i.e. oxytocin injection are carried out to Chinese snake head and green loach, the amount of oxytocin injection is every kilogram of Chinese snake head injection
The dosage of HCG is 165 international units；Inject LRH-A3Dosage be 4.9mg；The dosage for injecting DOM is 0.80mg；The note of green loach
Dosage is penetrated to halve；The time of certain oxytocin injection will be selected within the scope of the normal ovulation period of Chinese snake head and green loach；And
The Chinese snake head of oxytocin injection and the quantity ratio of green loach are 1:11.
After Chinese snake head and blue mud loach oxytocin injection 8h, i.e., its this when being ovulated, using it is artificial squeeze ovum by the way of,
The ovum for injecting the green loach of oxytocins is got into hybridization pond, was urged after the ovum of green loach gets into hybridization pond 9min, then by injecting
The sperm for producing the Chinese snake head of element is got into hybridization pond；Hybridization pond 8min is got into the ovum of green loach and the sperm of Chinese snake head
Afterwards, it is stirred for uniformly making its fertilization；Speed when stirring will maintain 25r/min slowly under normal circumstances, prevent mixing speed mistake
Fast to destroy fertilized eggs, mixing slowly can be such that the viscous fertilized eggs being linked togather separate, and can make sperm and ovum more fully
It contacts, after stopping 35min to be mixed, rinses fertilized eggs with clear water, make the viscous fertilized eggs being linked togather separate to form individual, be convenient for
Hatching；
Fertilized eggs are put into incubator and are hatched, fertilization pond can be directly placed into incubator carry out for convenience
Hatching, at 25 DEG C, the time is controlled in 68h for the temperature control of hatching；After hatching, loach seedling, which is carried out normal feed, is
It can.
Example IV
A kind of method of loach crossbreed, comprising the following steps:
Selection parent loach: select that Chinese snake head is male loach, green loach is female loach；Chinese snake head is mostly lived with the water surface
Based on, the comparison of the growth is fast but meat is bad, is easy to be endangered by birds and other natural enemies during the growth process, survival rate compares
It is low, but it has the advantages that growing way is fast；Green loach is mostly based on the life of mud bottom, and adaptability is stronger, and growing way is slow, and meat is fresh
Beauty causes to capture difficult because it is movable mostly in mud bottom；Chinese snake head is hybridized with green loach, it is faster than green loach to obtain growing way,
Based on living below the water surface, adaptability is relatively strong, delicious meat hybridization loach, is easy to be attacked by birds which overcome Chinese snake head
The drawbacks of hitting, also overcoming green loach, life is not easy the drawbacks of capturing at mud bottom；And growing way is fast, delicious flavour, adaptability is stronger,
It is suitble to large-scale farming, economic benefits comparison is high.
Chinese snake head and green loach are respectively put into the glass jar storage pond, holding pond sterilized and are cultivated, glass jar storage pond, holding pond makes
It is carried out disinfection with quick lime, and after disinfection 5 days, then injects water into storage pond, holding pond, be then respectively put into Chinese snake head and green loach
It is cultivated in storage pond, holding pond, to adapt it to the environment before hybridization, convenient for its oviposition；After being cultivated 3 days in storage pond, holding pond, respectively
Hasten parturition processing, i.e. oxytocin injection are carried out to Chinese snake head and green loach, the amount of oxytocin injection is every kilogram of Chinese snake head injection
The dosage of HCG is 170 international units；Inject LRH-A3Dosage be 5mg；The dosage for injecting DOM is 0.85mg；The injection of green loach
Dosage halves；The time of certain oxytocin injection will be selected within the scope of the normal ovulation period of Chinese snake head and green loach；And it infuses
The quantity ratio of the Chinese snake head and green loach of penetrating oxytocins is 1:12.
After Chinese snake head and blue mud loach oxytocin injection 9h, i.e., its this when being ovulated, using it is artificial squeeze ovum by the way of,
The ovum for injecting the green loach of oxytocins is got into hybridization pond, was urged after the ovum of green loach gets into hybridization pond 10min, then by injecting
The sperm for producing the Chinese snake head of element is got into hybridization pond；Hybridization pond 10min is got into the ovum of green loach and the sperm of Chinese snake head
Afterwards, it is stirred for uniformly making its fertilization；Speed when stirring will maintain 30r/min slowly under normal circumstances, prevent mixing speed mistake
Fast to destroy fertilized eggs, mixing slowly can be such that the viscous fertilized eggs being linked togather separate, and can make sperm and ovum more fully
It contacts, after stopping 40min to be mixed, rinses fertilized eggs with clear water, make the viscous fertilized eggs being linked togather separate to form individual, be convenient for
Hatching；
Fertilized eggs are put into incubator and are hatched, fertilization pond can be directly placed into incubator carry out for convenience
Hatching, at 28 DEG C, the time is controlled in 72h for the temperature control of hatching；After hatching, loach seedling, which is carried out normal feed, is
It can.
The comparison of hybridization loach of the present invention and Chinese snake head, green loach, as shown in table 1:
Table 1
As it can be seen from table 1 the life temperature of hybridization loach is more extensive, more adaptable；And hybridization loach avoids
Chinese snake head the water surface live, be easy food is invaded by bird the drawbacks of, greatly reduce the anti-bird management cost in incubation, also at
It avoids to function green loach to live in mud under water, captures difficult problem；In addition, hybridization loach combines Chinese snake head and blueness
The advantages of loach, growing way is moderate and meat taste is relatively slided tender tasty and refreshing, and price is higher than Chinese snake head and green loach in the market, i.e., simple
Changed feeding manner, value itself that reduce feeding cost and loach is improved, there are very big market prospects.
The present invention is not limited to above-mentioned preferred forms, anyone can show that other are various under the inspiration of the present invention
The product of form, however, make any variation in its details, it is all that there is technical solution identical or similar to the present application,
It is within the scope of the present invention.
Claims (10)
1. a kind of method of loach crossbreed, which comprises the following steps:
(1) Selection parent loach: select that Chinese snake head is male loach, green loach is female loach；
(2) Chinese snake head and green loach are respectively put into the storage pond, holding pond sterilized and are cultivated；
(3) it hastens parturition: using HCG, LRH-A3With the parent loach in DOM mixing oxytocin injections agent injection storage pond, holding pond；
(4) by the way of artificial crowded ovum, the ovum for injecting the green loach of oxytocins is got into hybridization pond, then will be injected again
The sperm of the Chinese snake head of oxytocins is got into hybridization pond, and is stirred evenly and made its fertilization, and fertilized eggs are obtained；
(5) fertilized eggs are put into incubator, hatch to obtain hybridization loach seedling.
2. a kind of method of loach crossbreed according to claim 1, which is characterized in that in step (2), with quick lime pair
Storage pond, holding pond carries out disinfection, and after 3-5 days to be sterilized, then water is injected into storage pond, holding pond, and Chinese snake head and green loach are respectively put into temporarily
It supports and is cultivated in pond.
3. a kind of method of loach crossbreed according to claim 1, which is characterized in that in step (3), every kilogram of Taiwan
The dosage of loach injection HCG is 160-170 international unit；Inject LRH-A3Dosage be 4.8-5mg；Injection DOM dosage be
0.78-0.85mg；The injection dosage of green loach halves.
4. a kind of method of loach crossbreed according to claim 1, which is characterized in that in step (4), to Chinese snake head
After blue mud loach oxytocin injection 6-9h, then carry out manually squeezing ovum.
5. a kind of method of loach crossbreed according to claim 1, which is characterized in that the ovum in step (4), to green loach
After getting into hybridization pond 8-10min, then the sperm of Chinese snake head is got into and hybridizes pond.
6. a kind of method of loach crossbreed according to claim 5, which is characterized in that the quantity of Chinese snake head and green loach
Than for 1:10-12.
7. a kind of method of loach crossbreed according to claim 1, which is characterized in that the ovum in step (4), to green loach
After getting into hybridization pond 5-10min with the sperm of Chinese snake head, it is stirred for uniformly making its fertilization.
8. a kind of loach cross breeding method according to claim 7, which is characterized in that in step (4), the speed of the stirring
Degree is 20-30r/min.
9. a kind of loach cross breeding method according to claim 1, which is characterized in that in step (4), stopping to be mixed
After 30-40min, fertilized eggs are rinsed with clear water, the fertilized eggs being bonded together is made to separate to form individual.
10. a kind of loach cross breeding method according to claim 1, which is characterized in that the ovum temperature of the ovum case
For 23-28 DEG C, the ovum time be 60-72h.
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Inventor after: Yang Ye Inventor after: Chen Bingsen Inventor after: Yang Yinbai Inventor after: Li Mingqiang Inventor after: Wang Tao Inventor after: Qiao Yanwen Inventor before: Yang Ye Inventor before: Chen Bingsen Inventor before: Yang Yinbai Inventor before: Wang Tao Inventor before: Qiao Yanwen |
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Application publication date: 20190104 |