CN104206323A - Taiwan loach hatching method - Google Patents
Taiwan loach hatching method Download PDFInfo
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- CN104206323A CN104206323A CN201410383062.5A CN201410383062A CN104206323A CN 104206323 A CN104206323 A CN 104206323A CN 201410383062 A CN201410383062 A CN 201410383062A CN 104206323 A CN104206323 A CN 104206323A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention provides a Taiwan loach hatching method, and relates to the field of loach culture. The method comprises the following steps that (1) healthy parent loaches with the age being 2 to 3 winters are selected; (2) the parent loaches are subjected to enhanced culture for 25 to 30 days; (3) chorionic gonadotrophin and DOM maleate are injected for the parent loaches, and the injection dosage does not exceed 0.5mm; (4) 0.5kg of fish roes and 1.8 to 2.2ml of sperm are put into 0.8 to 1.2kg of saline solution with the concentration being 0.3 percent to 0.5 percent for fertilization, and then, germ cells are put into a hatching barrel for hatching; (5) the parent loaches are put into a pool with fresh water quality to be fed, and in addition, the wound is subjected to medicine application, or antibacterials is injected; (6) larval fish is put into the pool after being hatched for 70 to 80 hours, cooked egg yolk is thrown for feeding the fish, and before the throwing feeding, the cooked egg yolk residue thrown and fed in the last time needs to be cleaned. The Taiwan loach hatching method provided by the invention has the advantages that the implementation is easy, the fertility rate and the hatchability can reach more than 95 percent, the survival rate is high, and the large-scale hatching culture of the Taiwan loaches can be favorably realized.
Description
Technical field
The present invention relates to loach breeding field, particularly relate to a kind of Chinese snake head hatching m.
Background technology
Chinese snake head is negligible amounts in natural water area, and close loach ovary, testis development are inconsistent, and synchronous spawning amount is on the low side, and hatching process is subject to the external environment impacts such as the on the low side and cyclops infringement of wind-force, temperature, heavy rain and water body dissolved oxygen amount.And during artificial propagation Chinese snake head, water quality and temperature control bad, the feeding volume of feed is held inaccurate, and cultivation density crosses the problems such as high, thus incubation rate and survival rate on the low side.
Summary of the invention
Incubation rate and survival rate problem on the low side when the object of the invention is to solve current artificial propagation Chinese snake head, provide a kind of Chinese snake head hatching m, the method is easy to implement, and fertility rate and hatchability can reach more than 95%, survival rate is high, is conducive to the extensive hatching cultivation realizing Chinese snake head.
For achieving the above object, technical scheme provided by the invention is as follows:
The step of Chinese snake head hatching m provided by the invention is as follows:
(1) close loach is selected
Select 2 ~ 3 age in winter physique healthy and strong, body colour is normal, the bodily form is rectified, energetic, without disability, health parent loach that fin ray is complete, male close loach requires body length 15 ~ 20cm, body weight 12 ~ 40g, and dam loach reaches 40 ~ 60g;
(2) reinforced cultivating of close loach
Dam loach and hero parent loach are divided to open in pond and carries out bath cultivation, time is 25 ~ 30 days, the pond degree of depth is 1.2 ~ 1.5 meters, the pH value of water remains between 7 ~ 8, keep the mud of 8 ~ 14 centimetres at the bottom of pond, breeding density is every mu and puts 100 ~ 150Kg in a suitable place to breed, reinforced cultivating feed of throwing something and feeding every day, morning and afternoon respectively throws once, and each feeding volume is 7% ~ 9% of close loach body weight;
(3) injection is hastened parturition
For dam loach and hero parent loach injection oxytocic drug, the dam loach of every 10 kilograms joins the hero parent loach of 0.4 ~ 0.6 kilogram, injecting method is the shot of dorsal fin base portion muscle, inject time select at night 7 ~ 8 time, oxytocic drug is human chorionic gonadtropin and maleic acid DOM, the human chorionic gonadtropin injection volume of dam loach is 15 ~ 20IU/g, the injection volume of maleic acid DOM is 0.004 ~ 0.006mg/g, the dosage of male close loach reduces by half, and the injected dose of every tail parent loach is no more than 0.5 milliliter; Water temperature controls at 22 DEG C ~ 25 DEG C;
(4) artificial insemination and hatching
After injection oxytocic drug after 12 ~ 15 hours, manual compression goes out the sperm of the fish-egg of dam loach and hero parent loach, and it is be fertilized in the salt solution of 0.3% ~ 0.5% that the sperm of the fish-egg of 0.5 kilogram and 1.8 ~ 2.2 milliliters is put into 0.8 ~ 1.2 kilogram of concentration;
After 8 ~ 12 minutes, wash away salt solution, then hatching pail fertilized egg putting into being equipped with clear water is hatched, hatching density is generally in every premium on currency puts 300 ~ 500, fertilized egg, and by the tracheae that is connected with the air pump bottom delivery air from hatching pail, air is up emerged from the bottom of hatching pail, and incubation temperature controls, at 24 DEG C ~ 26 DEG C, after 25 ~ 30 hours, to hatch to obtain prelarva;
(5) nursing of close loach
The close loach being extruded fish-egg and sperm is put in the pure and fresh pond of water quality and feeds, for injured close loach, according to injured degree in wound coating medicine or injection antibacterials: wound embrocated by the following medicine of selecting of wound that close loach is slight: liquor potassic permanganate, sulfanilamide (SN) ointment, penicillin ointment or nitrofurazone ointment; Badly-wounded close loach, except embrocating anti-inflammation drugs, also will inject sulfanilamide (SN) azoles sodium or penicillin for animals; Injured very serious close loach does to eliminate process;
(6) larvae cultivation
Continue after prelarva hatching to cultivate 70 ~ 80 hours in hatching pail, then prelarva is put into pond, and start ripe yolk of throwing something and feeding, every 100,000 tail prelarvas are thrown something and fed a ripe yolk, morning and afternoon respectively throws once, ripe yolk hand is crumbed and to be thrown something and fed after 120 order silk cover filterings again, will remove residue before within second day, throwing something and feeding.
Especially, described reinforced cultivating feed be earthworm, fly maggot, poultry offcuts, dregs of beans, wheat bran or the protein content loach material that is greater than 40%.
Beneficial effect of the present invention:
Chinese snake head hatching m provided by the invention strictly selects healthy close loach, control the feeding volume of temperature, breeding density and the feed cultivated, result of study shows: fertility rate and hatchability can reach more than 95%, 3 centimetres can be grown to after prelarva cultivates 15 ~ 20 days in pond, survival rate is high, and the method is easy to implement, be conducive to the extensive hatching cultivation realizing Chinese snake head.
Embodiment
The present invention is described further below, and the present embodiment only represents one or more preferred forms, should not be construed as limiting the invention.
Embodiment 1:
The step of Chinese snake head hatching m provided by the invention is as follows:
(1) close loach is selected
Select 2 ~ 3 age in winter physique healthy and strong, body colour is normal, the bodily form is rectified, energetic, without disability, health parent loach that fin ray is complete, male close loach requires body length 15 ~ 20cm, body weight 12 ~ 40g, and dam loach reaches 40 ~ 60g.
(2) reinforced cultivating of close loach
Dam loach and hero parent loach are divided to open in pond and carries out bath cultivation, time is 25 days, the pond degree of depth is 1.2 meters, the pH value of water remains between 7 ~ 8, keeps the mud of 14 centimetres at the bottom of pond, and breeding density is every mu and puts 100Kg in a suitable place to breed, to throw something and feed every day reinforced cultivating feed, the loach material that reinforced cultivating feed is earthworm, fly maggot, poultry offcuts, dregs of beans, wheat bran or protein content are greater than 40%, morning and afternoon respectively throws once, and each feeding volume is 7% of close loach body weight.
(3) injection is hastened parturition
For dam loach and hero parent loach injection oxytocic drug, the dam loach of every 10 kilograms joins the hero parent loach of 0.4 kilogram, injecting method is the shot of dorsal fin base portion muscle, inject time select at night 7 ~ 8 time, oxytocic drug is human chorionic gonadtropin and maleic acid DOM, and the human chorionic gonadtropin injection volume of dam loach is 15IU/g, and the injection volume of maleic acid DOM is 0.004mg/g, the dosage of male close loach reduces by half, and the injected dose of every tail parent loach is no more than 0.5 milliliter; Water temperature controls at 22 DEG C.
(4) artificial insemination and hatching
After injection oxytocic drug after 15 hours, manual compression goes out the sperm of the fish-egg of dam loach and hero parent loach, and it is be fertilized in the salt solution of 0.4% that the sperm of the fish-egg of 0.5 kilogram and 1.8 milliliters is put into 0.8 kilogram of concentration.
After 12 minutes, wash away salt solution, then hatching pail fertilized egg putting into being equipped with clear water is hatched, hatching density is generally in every premium on currency puts 300, fertilized egg, and by the tracheae that is connected with the air pump bottom delivery air from hatching pail, air is up emerged from the bottom of hatching pail, and incubation temperature controls, at 24 DEG C, after 30 hours, to hatch to obtain prelarva.
Wherein, the bottom of hatching pail is funnel-form, and one end that lower diameter is larger is the openend of bottom, the top of hatching pail is cylindrical, and the one end on top is connected with the openend of bottom, and the diameter on top is 2m, be highly 50 centimeters, the air-out of described tracheae is positioned at the bottom of hatching pail.
(5) nursing of close loach
The close loach being extruded fish-egg and sperm is put in the pure and fresh pond of water quality and feeds, for injured close loach, medicine or injection antibacterials should be applied according to injured degree in wound: wound embrocated by the following medicine of can selecting of wound that close loach is slight: liquor potassic permanganate, sulfanilamide (SN) ointment, penicillin ointment or nitrofurazone ointment; Badly-wounded close loach, except embrocating anti-inflammation drugs, also will inject sulfanilamide (SN) azoles sodium or penicillin for animals; Injured very serious close loach does to eliminate process.
(6) larvae cultivation
Continue after prelarva hatching to cultivate 70 hours in hatching pail, then prelarva is put into pond, and start ripe yolk of throwing something and feeding, every 100,000 tail prelarvas are thrown something and fed a ripe yolk, morning and afternoon respectively throws once, and ripe yolk hand is crumbed and to be thrown something and fed after 120 order silk cover filterings again, will remove residue before within second day, throwing something and feeding.
Embodiment 2:
The step of Chinese snake head hatching m provided by the invention is as follows:
(1) close loach is selected
Select 2 ~ 3 age in winter physique healthy and strong, body colour is normal, the bodily form is rectified, energetic, without disability, health parent loach that fin ray is complete, male close loach requires body length 15 ~ 20cm, body weight 12 ~ 40g, and dam loach reaches 40 ~ 60g.
(2) reinforced cultivating of close loach
Dam loach and hero parent loach are divided to open in pond and carries out bath cultivation, time is 30 days, the pond degree of depth is 1.5 meters, the pH value of water remains between 7 ~ 8, keeps the mud of 10 centimetres at the bottom of pond, and breeding density is every mu and puts 150Kg in a suitable place to breed, to throw something and feed every day reinforced cultivating feed, the loach material that reinforced cultivating feed is earthworm, fly maggot, poultry offcuts, dregs of beans, wheat bran or protein content are greater than 40%, morning and afternoon respectively throws once, and each feeding volume is 8% of close loach body weight.
(3) injection is hastened parturition
For dam loach and hero parent loach injection oxytocic drug, the dam loach of every 10 kilograms joins the hero parent loach of 0.5 kilogram, injecting method is the shot of dorsal fin base portion muscle, inject time select at night 7 ~ 8 time, oxytocic drug is human chorionic gonadtropin and maleic acid DOM, and the human chorionic gonadtropin injection volume of dam loach is 18IU/g, and the injection volume of maleic acid DOM is 0.005mg/g, the dosage of male close loach reduces by half, and the injected dose of every tail parent loach is no more than 0.5 milliliter; Water temperature controls at 25 DEG C.
(4) artificial insemination and hatching
After injection oxytocic drug after 12 hours, manual compression goes out the sperm of the fish-egg of dam loach and hero parent loach, and it is be fertilized in the salt solution of 0.4% that the sperm of the fish-egg of 0.5 kilogram and 2 milliliters is put into 1 kilogram of concentration.
After 10 minutes, wash away salt solution, then hatching pail fertilized egg putting into being equipped with clear water is hatched, hatching density is generally in every premium on currency puts 400, fertilized egg, and by the tracheae that is connected with the air pump bottom delivery air from hatching pail, air is up emerged from the bottom of hatching pail, and incubation temperature controls, at 25 DEG C, after 28 hours, to hatch to obtain prelarva.
Wherein, the bottom of hatching pail is funnel-form, and one end that lower diameter is larger is the openend of bottom, the top of hatching pail is cylindrical, and the one end on top is connected with the openend of bottom, and the diameter on top is 2m, be highly 50 centimeters, the air-out of described tracheae is positioned at the bottom of hatching pail.
(5) nursing of close loach
The close loach being extruded fish-egg and sperm is put in the pure and fresh pond of water quality and feeds, for injured close loach, medicine or injection antibacterials should be applied according to injured degree in wound: wound embrocated by the following medicine of can selecting of wound that close loach is slight: liquor potassic permanganate, sulfanilamide (SN) ointment, penicillin ointment or nitrofurazone ointment; Badly-wounded close loach, except embrocating anti-inflammation drugs, also will inject sulfanilamide (SN) azoles sodium or penicillin for animals; Injured very serious close loach does to eliminate process.
(6) larvae cultivation
Continue after prelarva hatching to cultivate 75 hours in hatching pail, then prelarva is put into pond, and start ripe yolk of throwing something and feeding, every 100,000 tail prelarvas are thrown something and fed a ripe yolk, morning and afternoon respectively throws once, and ripe yolk hand is crumbed and to be thrown something and fed after 120 order silk cover filterings again, will remove residue before within second day, throwing something and feeding.
Embodiment 3:
The step of Chinese snake head hatching m provided by the invention is as follows:
(1) close loach is selected
Select 2 ~ 3 age in winter physique healthy and strong, body colour is normal, the bodily form is rectified, energetic, without disability, health parent loach that fin ray is complete, male close loach requires body length 15 ~ 20cm, body weight 12 ~ 40g, and dam loach reaches 40 ~ 60g.
(2) reinforced cultivating of close loach
Dam loach and hero parent loach are divided to open in pond and carries out bath cultivation, time is 25 ~ 30 days, the pond degree of depth is 1.5 meters, the pH value of water remains between 7 ~ 8, keeps the mud of 8 centimetres at the bottom of pond, and breeding density is every mu and puts 150Kg in a suitable place to breed, to throw something and feed every day reinforced cultivating feed, the loach material that reinforced cultivating feed is earthworm, fly maggot, poultry offcuts, dregs of beans, wheat bran or protein content are greater than 40%, morning and afternoon respectively throws once, and each feeding volume is 9% of close loach body weight.
(3) injection is hastened parturition
For dam loach and hero parent loach injection oxytocic drug, injecting method is the shot of dorsal fin base portion muscle, inject time select at night 7 ~ 8 time, oxytocic drug is human chorionic gonadtropin and maleic acid DOM, the human chorionic gonadtropin injection volume of dam loach is 20IU/g, the injection volume of maleic acid DOM is 0.006mg/g, and the dosage of male close loach reduces by half, and the injected dose of every tail parent loach is no more than 0.5 milliliter; Water temperature controls at 25 DEG C, and the dam loach of every 10 kilograms joins the hero parent loach of 0.6 kilogram.
(4) artificial insemination and hatching
After injection oxytocic drug after 14 hours, manual compression goes out the sperm of the fish-egg of dam loach and hero parent loach, and it is be fertilized in the salt solution of 0.4% that the sperm of the fish-egg of 0.5 kilogram and 2.2 milliliters is put into 1.2 kilograms of concentration.
After 8 minutes, wash away salt solution, then hatching pail fertilized egg putting into being equipped with clear water is hatched, hatching density is generally in every premium on currency puts 500, fertilized egg, and by the tracheae that is connected with the air pump bottom delivery air from hatching pail, air is up emerged from the bottom of hatching pail, and incubation temperature controls, at 26 DEG C, after 25 hours, to hatch to obtain prelarva.
Wherein, the bottom of hatching pail is funnel-form, and one end that lower diameter is larger is the openend of bottom, the top of hatching pail is cylindrical, and the one end on top is connected with the openend of bottom, and the diameter on top is 2m, be highly 50 centimeters, the air-out of described tracheae is positioned at the bottom of hatching pail.
(5) nursing of close loach
The close loach being extruded fish-egg and sperm is put in the pure and fresh pond of water quality and feeds, for injured close loach, medicine or injection antibacterials should be applied according to injured degree in wound: wound embrocated by the following medicine of can selecting of wound that close loach is slight: liquor potassic permanganate, sulfanilamide (SN) ointment, penicillin ointment or nitrofurazone ointment; Badly-wounded close loach, except embrocating anti-inflammation drugs, also will inject sulfanilamide (SN) azoles sodium or penicillin for animals; Injured very serious close loach does to eliminate process.
(6) larvae cultivation
Continue after prelarva hatching to cultivate 80 hours in hatching pail, then prelarva is put into pond, and start ripe yolk of throwing something and feeding, every 100,000 tail prelarvas are thrown something and fed a ripe yolk, morning and afternoon respectively throws once, and ripe yolk hand is crumbed and to be thrown something and fed after 120 order silk cover filterings again, will remove residue before within second day, throwing something and feeding.
Result of study shows: fertility rate and hatchability can reach more than 95%, and can grow to 3 centimetres after prelarva cultivates 15 ~ 20 days in pond, survival rate is high.
Claims (3)
1. a Chinese snake head hatching m, is characterized in that: step is as follows:
(1) close loach is selected
Select 2 ~ 3 age in winter physique healthy and strong, body colour is normal, the bodily form is rectified, energetic, without disability, health parent loach that fin ray is complete, male close loach requires body length 15 ~ 20cm, body weight 12 ~ 40g, and dam loach reaches 40 ~ 60g;
(2) reinforced cultivating of close loach
Dam loach and hero parent loach are divided to open in pond and carries out bath cultivation, time is 25 ~ 30 days, the pond degree of depth is 1.2 ~ 1.5 meters, the pH value of water remains between 7 ~ 8, keep the mud of 8 ~ 14 centimetres at the bottom of pond, breeding density is every mu and puts 100 ~ 150Kg in a suitable place to breed, reinforced cultivating feed of throwing something and feeding every day, morning and afternoon respectively throws once, and each feeding volume is 7% ~ 9% of close loach body weight;
(3) injection is hastened parturition
For dam loach and hero parent loach injection oxytocic drug, the dam loach of every 10 kilograms joins the hero parent loach of 0.4 ~ 0.6 kilogram, injecting method is the shot of dorsal fin base portion muscle, inject time select at night 7 ~ 8 time, oxytocic drug is human chorionic gonadtropin and maleic acid DOM, the human chorionic gonadtropin injection volume of dam loach is 15 ~ 20IU/g, the injection volume of maleic acid DOM is 0.004 ~ 0.006mg/g, the dosage of male close loach reduces by half, and the injected dose of every tail parent loach is no more than 0.5 milliliter; Water temperature controls at 22 DEG C ~ 25 DEG C;
(4) artificial insemination and hatching
After injection oxytocic drug after 12 ~ 15 hours, manual compression goes out the sperm of the fish-egg of dam loach and hero parent loach, and it is be fertilized in the salt solution of 0.3% ~ 0.5% that the sperm of the fish-egg of 0.5 kilogram and 1.8 ~ 2.2 milliliters is put into 0.8 ~ 1.2 kilogram of concentration;
After 8 ~ 12 minutes, wash away salt solution, then hatching pail fertilized egg putting into being equipped with clear water is hatched, hatching density is generally in every premium on currency puts 300 ~ 500, fertilized egg, and by the tracheae that is connected with the air pump bottom delivery air from hatching pail, air is up emerged from the bottom of hatching pail, and incubation temperature controls, at 24 DEG C ~ 26 DEG C, after 25 ~ 30 hours, to hatch to obtain prelarva;
(5) nursing of close loach
The close loach being extruded fish-egg and sperm is put in the pure and fresh pond of water quality and feeds, for injured close loach, according to injured degree in wound coating medicine or injection antibacterials: wound embrocated by the following medicine of selecting of wound that close loach is slight: liquor potassic permanganate, sulfanilamide (SN) ointment, penicillin ointment or nitrofurazone ointment; Badly-wounded close loach, except embrocating anti-inflammation drugs, also will inject sulfanilamide (SN) azoles sodium or penicillin for animals; Injured very serious close loach does to eliminate process;
(6) larvae cultivation
Continue after prelarva hatching to cultivate 70 ~ 80 hours in hatching pail, then prelarva is put into pond, and start ripe yolk of throwing something and feeding, every 100,000 tail prelarvas are thrown something and fed a ripe yolk, morning and afternoon respectively throws once, ripe yolk hand is crumbed and to be thrown something and fed after 120 order silk cover filterings again, will remove residue before within second day, throwing something and feeding.
2. Chinese snake head hatching m according to claim 1, it is characterized in that: the bottom of described hatching pail is funnel-form, one end that lower diameter is larger is the openend of bottom, the top of hatching pail is cylindrical, the one end on top is connected with the openend of bottom, the diameter on top is 2m, is highly 50 centimeters, and the air-out of described tracheae is positioned at the bottom of hatching pail.
3. Chinese snake head hatching m according to claim 1, is characterized in that: the loach material that described reinforced cultivating feed is earthworm, fly maggot, poultry offcuts, dregs of beans, wheat bran or protein content are greater than 40%.
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| CN105532520A (en) * | 2015-12-07 | 2016-05-04 | 内江师范学院 | Sinibotiareevesa seedling breeding method |
| CN105532533A (en) * | 2015-12-29 | 2016-05-04 | 太和县旭升水产养殖有限公司 | Fry cultivating method for loaches |
| CN105594636A (en) * | 2015-12-19 | 2016-05-25 | 太和县顺发养殖有限公司 | Efficient artificial culture method for good-quality loach |
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| CN107711611A (en) * | 2017-10-17 | 2018-02-23 | 铜仁职业技术学院 | A kind of low damage of Chinese snake head, high-efficiency artificial propagation method |
| CN108094265A (en) * | 2017-11-16 | 2018-06-01 | 孝昌县建新泥鳅专业合作社 | A kind of method of Chinese snake head seed rearing |
| CN109122419A (en) * | 2018-09-12 | 2019-01-04 | 河南昌佳农业发展有限公司 | A kind of method of loach crossbreed |
| CN109699530A (en) * | 2019-01-25 | 2019-05-03 | 沧州北通达海洋工程机械科技有限公司 | Loach breeding induced spawning method |
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| CN105532520A (en) * | 2015-12-07 | 2016-05-04 | 内江师范学院 | Sinibotiareevesa seedling breeding method |
| CN105532520B (en) * | 2015-12-07 | 2019-06-04 | 内江师范学院 | A kind of expanded letter sand loach seedlings kind propagation method |
| CN105475197A (en) * | 2015-12-19 | 2016-04-13 | 太和县顺发养殖有限公司 | Loach larva incubation and cultivation method |
| CN105594636A (en) * | 2015-12-19 | 2016-05-25 | 太和县顺发养殖有限公司 | Efficient artificial culture method for good-quality loach |
| CN105613366A (en) * | 2015-12-19 | 2016-06-01 | 太和县顺发养殖有限公司 | Efficient spawning induction method of misgurnus anguillicaudatus |
| CN105532533A (en) * | 2015-12-29 | 2016-05-04 | 太和县旭升水产养殖有限公司 | Fry cultivating method for loaches |
| CN107711611A (en) * | 2017-10-17 | 2018-02-23 | 铜仁职业技术学院 | A kind of low damage of Chinese snake head, high-efficiency artificial propagation method |
| CN108094265A (en) * | 2017-11-16 | 2018-06-01 | 孝昌县建新泥鳅专业合作社 | A kind of method of Chinese snake head seed rearing |
| CN109122419A (en) * | 2018-09-12 | 2019-01-04 | 河南昌佳农业发展有限公司 | A kind of method of loach crossbreed |
| CN109699530A (en) * | 2019-01-25 | 2019-05-03 | 沧州北通达海洋工程机械科技有限公司 | Loach breeding induced spawning method |
| CN109699530B (en) * | 2019-01-25 | 2021-09-07 | 佛山市嘉沃农业科技合伙企业（有限合伙） | Artificial spawning induction method for loach culture |
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