CN108541632A - A kind of sturgeon mating system - Google Patents
A kind of sturgeon mating system Download PDFInfo
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- CN108541632A CN108541632A CN201810481217.7A CN201810481217A CN108541632A CN 108541632 A CN108541632 A CN 108541632A CN 201810481217 A CN201810481217 A CN 201810481217A CN 108541632 A CN108541632 A CN 108541632A
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- sturgeon
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- 241000881711 Acipenser sturio Species 0.000 title claims abstract description 43
- 230000013011 mating Effects 0.000 title claims abstract description 13
- 235000013601 eggs Nutrition 0.000 claims abstract description 26
- 238000009360 aquaculture Methods 0.000 claims abstract description 25
- 244000144974 aquaculture Species 0.000 claims abstract description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 25
- 241000238557 Decapoda Species 0.000 claims abstract description 17
- 210000004027 cell Anatomy 0.000 claims abstract description 14
- 239000003814 drug Substances 0.000 claims abstract description 12
- 229940079593 drug Drugs 0.000 claims abstract description 12
- 230000032696 parturition Effects 0.000 claims abstract description 12
- 230000012447 hatching Effects 0.000 claims abstract description 10
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 8
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 8
- 210000004681 ovum Anatomy 0.000 claims abstract description 8
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 5
- 230000026109 gonad development Effects 0.000 claims abstract description 5
- 229940088597 hormone Drugs 0.000 claims abstract description 5
- 239000005556 hormone Substances 0.000 claims abstract description 5
- 230000009027 insemination Effects 0.000 claims abstract description 5
- 230000001592 luteinising effect Effects 0.000 claims abstract description 5
- 210000000582 semen Anatomy 0.000 claims abstract description 5
- 238000002347 injection Methods 0.000 claims description 19
- 239000007924 injection Substances 0.000 claims description 19
- 241000196324 Embryophyta Species 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 11
- 210000003205 muscle Anatomy 0.000 claims description 6
- 241000241034 Palaemon pugio Species 0.000 claims description 4
- 241001123263 Zostera Species 0.000 claims description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 4
- 229910052760 oxygen Inorganic materials 0.000 claims description 4
- 239000001301 oxygen Substances 0.000 claims description 4
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 claims description 3
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 claims description 3
- 239000013505 freshwater Substances 0.000 claims description 3
- 238000005457 optimization Methods 0.000 abstract 1
- 230000012010 growth Effects 0.000 description 14
- 230000000052 comparative effect Effects 0.000 description 7
- 210000004907 gland Anatomy 0.000 description 7
- 230000001568 sexual effect Effects 0.000 description 7
- 238000009395 breeding Methods 0.000 description 6
- 230000001488 breeding effect Effects 0.000 description 6
- 241000251468 Actinopterygii Species 0.000 description 5
- 241001284241 Metapenaeus bennettae Species 0.000 description 4
- 241001533364 Portunus trituberculatus Species 0.000 description 3
- 210000001015 abdomen Anatomy 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000016087 ovulation Effects 0.000 description 3
- 241000252335 Acipenser Species 0.000 description 2
- 241000276446 Gadiformes Species 0.000 description 2
- 230000004720 fertilization Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 241000251464 Coelacanthiformes Species 0.000 description 1
- 101100136092 Drosophila melanogaster peng gene Proteins 0.000 description 1
- 241001263748 Pityopsis Species 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 206010016165 failure to thrive Diseases 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 210000005239 tubule Anatomy 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
This programme discloses a kind of sturgeon mating system of sturgeon cultivation technical field, includes the following steps：One, the excellent sturgeon parent population of character is chosen；1~2 year before take ovum/essence, parent population is launched by gender and carries out a point pond into aquaculture pond and raises；The bottom of aquaculture pond is laid with silt, and filter screen is covered on silt, the cobblestone of 2~3CM thickness is laid on filter screen；Launch submerged plant and shrimp crab in aquaculture pond, two, after parent population gonad development reaches the IV phases, drug is carried out to parent population using luteinising hormone-releasing hormo and is hastened parturition, dosage is：1.2~1.5 μ g/kg of milter, 2.5~3 μ g/kg of raun；Drug is hastened parturition during the previous moon to egg cell/seminal fluid collecting finishes, and aquaculture pond uses circulating water；Step 3: the egg cell and sperm to collection are carried out using artificial insemination；Then fertilized eggs are put into incubator and are hatched, until seedling incubating is gone out.This programme improves the hatching rate and quality of sturgeon by the mating system of optimization sturgeon.
Description
Technical field
The invention belongs to sturgeon cultivation technical field, more particularly to a kind of sturgeon mating system.
Background technology
Sturgeon refers to the fish of Gadiformes, and sturgeon is under the jurisdiction of Osteichthyes, Actinopterygii, hard shell catalogue, Gadiformes.Sturgeon
Individual big, growth is fast, not only has edible, nutrition, nourishing, health care, medicinal and ornamental etc. important economic value, but also have
There is important scientific research value.
With the fast development of sturgeon industry, sturgeon artificial breeding technology reaches its maturity.Such as application No. is
The Chinese invention patent of CN201110147872.7 discloses a kind of sturgeon artificial fecundation method, but the invention one is to use sign
Female male parent is differentiated in observation, can not thoroughly be solved female male parent in parent population selection and is difficult to resolution problems；Second is that the patent is in parent population
The cooling mode under natural conditions is not met using the method that parent is directly transferred to low temperature environment, the method in cultivation, simultaneously
How raised temperature is after not illustrating low temperature cultivation；Third, the patent can not accurately select of the right age parent to carry out artificial breeding
It educates.
For such case, application No. is the patents of " CN201410257306.5 " to disclose a kind of " mountain area sturgeon parent
Cultivation and induced spawning method ", this method emphatically distinguishes sturgeon parent population, and is marked in detail to the growing state of parent population
Note, and reinforce artificial induced spawning and artificial insemination, and then improve the fertility rate and hatchability of sturgeon.
Although aforesaid way all improves the hatching rate of sturgeon to a certain extent, sturgeon hatching rate and hatch
Nursery health condition, it is closely bound up with the quality of sturgeon parent population egg cell and sperm.And above-mentioned breeding way lacks to sturgeon
Fish parent fish cultivation is paid close attention to.
Invention content
The invention is intended to provide a kind of sturgeon mating system, by the breeding way for optimizing sturgeon parent population, to improve sturgeon
Parent population egg cell and sperm quality, and then improve the hatching rate and quality of sturgeon.
A kind of sturgeon mating system in this programme, includes the following steps：
Step 1: choosing the excellent sturgeon parent population of character；1~2 year before take ovum/essence, parent population is launched by gender
It is raised to a point pond is carried out in aquaculture pond；Parent population injected volume is 4~5kg/ ㎡；Aquaculture pond bottom is in 8~12 ° of inclination angle, aquaculture pond
Bottom be equipped with the silt of 5~6CM thickness, filter screen is covered on silt, the cobblestone of 2~3CM thickness is laid on filter screen；It is supporting
It grows and launches submerged plant and shrimp crab, aquaculture pond 1.8~2m of the depth of water, 7~10mg/L of dissolved oxygen in pond；Keep water temperature 4~October
15~18 DEG C, other months keep 11~14 DEG C of water temperature；Feedstuff feeding amount is to press the 1~1.5% of parent population weight daily；
Step 2: after parent population gonad development reaches the IV phases, drug is carried out to parent population using luteinising hormone-releasing hormo and is urged
Production, specially：Muscle of back injection is carried out using double injection method to female parent population, injection dosage is 1 μ g/ when hastening parturition for the first time
Kg, injection dosage is 1.5~2 μ g/kg when injecting for the second time, and interval time is 12~14h；Isotope labeled leucine is used to male parent population
Muscle of back injection is carried out, injection dosage is 1.2~1.5 μ g/kg.；The drug previous moon of hastening parturition is complete to egg cell/seminal fluid collecting
During finishing, aquaculture pond uses circulating water；
Step 3: the egg cell and sperm to collection are carried out using artificial insemination；Then fertilized eggs are put into incubator
Hatched, until seedling incubating is gone out；During incubating oosperm, in the premise for ensureing no ovum outflow incubator
Under, keep fertilized eggs to roll up and down using flow.
The operation principle and advantage of this programme：1~2 year before parent population artificial breeding, parent population is put into cultivation
It is cultivated in pond, there is silt, silt to contribute to the growth of submerged plant due to being launched in aquaculture pond；And submerged plant carries for aquaculture pond
Oxygen is conducive to parent population growth.At the same time, submerged plant and silt can promote the growth of microorganism and planktonic organism, float
Trip biology and microorganism provide nutrient for shrimp crab, promote growth, the addition of shrimp crab is conducive to parent population activity, and then increases its fortune
Momentum improves parent population constitution.In addition, the whole lower temperature at 11~18 DEG C of water temperature, helps avoid submerged plant and shrimp crab
Mass propagation, help to ensure that the integrated environment of aquaculture pond.And increase filter screen on silt, it is big on the one hand to avoid silt
Amount flow to above cobblestone and is polluted to water body, while limiting the growth of submerged plant, and excessive submerged plant is avoided to cause
Water pollution；Cobblestone is while limiting submerged plant growth, since sturgeon is liked moving about in the bottom of pond or pool wall of aquaculture pond,
In the contact process of cobblestone and sturgeon, is conducive to the germ for removing abdomen, while stimulating sexual gland, is conducive to the normal of its sexual gland
Growth.
And in 4~October, 15~18 DEG C of water temperature is kept, is more than the consumption master of sturgeon nutriment after 15 DEG C in water temperature
If the growth of individual, but too fast growth can lead to sexual gland growth failure, and keeping the temperature at suitable temperature makes sturgeon
The speed of growth controls in suitable section, and it is too fast or excessively slow to avoid growth；Other months keep 11~14 DEG C of water temperature；To sturgeon
The sexual gland of fish parent fish is stimulated, and ensures its sexual gland healthy growth.
It hastens parturition during the previous moon to egg cell/seminal fluid collecting finishes in drug, aquaculture pond uses circulating water, circulating water to have
The development for helping promote parent population sexual gland, makes its gonad development accelerate；Along with the drug of luteinising hormone-releasing hormo is hastened parturition, make
It obtains parent population ovulation/essence to shift to an earlier date, avoids parent population ovulation/essence too late, cause parent population in later stage backing off of physical function ovulation/essence, shadow
The problem of ringing egg cell/sperm quality occurs.
This programme increases its amount of exercise, ensures the normal development of parent population sexual gland, to carry by improving parent population growing environment
The quality of high parent population egg cell/sperm, and then improve the hatching rate and quality of sturgeon.
Further, in step 1, the submerged plant is at least one of Mo Si and eel grass.Not in silk and eel grass extremely
Few one kind all having preferable water purification effect, and inhibited to the growth of germ.
Further, in step 1, the shrimp is at least one of freshwater shrimp, river prawn, grass shrimp and prawn.Freshwater shrimp, river prawn, grass
Shrimp and prawn are omnivorousness shrimps, can be food with water plant, while can also reduce feed extra in aquaculture pond.
Further, in step 1, the crab is to close at least one of public crab, swimming crab and river crab.Close public crab, swimming crab
It is smaller with river crab build, at the same suitable for grown in river water；It has purified water quality, together durings edible microorganismus etc.
When but also as sturgeon food.
Further, the drug the specific steps are：Muscle of back injection is carried out using double injection method to female parent population, the
Primary injection dosage when hastening parturition is 1 μ g/kg, and injection dosage is 1.5~2 μ g/kg when injecting for second, interval time is 12~
14h；Muscle of back injection is carried out using isotope labeled leucine to male parent population, injection dosage is 1.2~1.5 μ g/kg.Female parent population injection
It measures more, takes double injection, be conducive to the healthy growth of female parent population.
Further, it in step 3, during incubating oosperm, under the premise of ensureing that no ovum flows out incubator, utilizes
Flow keeps fertilized eggs to roll up and down.It is rolled by flow cavitation result fertilized eggs, advantageously reduces the adhesive between fertilized eggs, simultaneously
The probability that the germs such as water mo(u)ld adhere on fertilized eggs is reduced, and then improves the hatching rate of fertilized eggs.
Specific implementation mode
It is further described below by specific implementation mode：
Embodiment 1：
A kind of sturgeon mating system, it is characterised in that：Include the following steps：
Step 1: choosing the excellent acipenser schrencki parent population of character；1~2 year before take ovum/essence, parent population is thrown by gender
It is put into aquaculture pond and carries out a point pond and raise；Parent population injected volume is 4~5kg/ ㎡；Aquaculture pond bottom is in 8~12 ° of inclination angle, cultivation
The bottom in pond is equipped with the silt of 5~6CM thickness, and filter screen is covered on silt, the cobblestone of 2~3CM thickness is laid on filter screen；
Launched in aquaculture pond not silk, eel grass, river prawn, grass shrimp, river prawn, prawn, close public crab, swimming crab and river crab, the aquaculture pond depth of water 1.8~
2m, 7~10mg/L of dissolved oxygen；15~18 DEG C of water temperature, other months is kept to keep 11~14 DEG C of water temperature 4~October；Feed is thrown
Amount is fed to press the 1~1.5% of parent population weight daily；
Step 2: parent population gonad development reaches the IV phases, (IV phases, male spermaries have an apparent vascular distribution, in fine tubule
There are sperm mother cells at different levels.Female abdomen Peng is big, and it is hard to press abdomen feel) after, using luteinising hormone-releasing hormo to parent
Fish carries out drug and hastens parturition, and dosage is：1.2~1.5 μ g/kg of milter, 2.5~3 μ g/kg of raun；Drug hastens parturition the previous moon to ovum
During cell/seminal fluid collecting finishes, aquaculture pond uses circulating water；
Step 3: the egg cell and sperm to collection are carried out using artificial insemination；Then fertilized eggs are put into incubator
Hatched, until seedling incubating is gone out.
Embodiment 2 the difference is that only that parent population is siberia platform with embodiment 1.
Comparative example 1, using application No. is method progress disclosed in embodiment in the patent of " CN201410257306.5 " is numerous
It educates, parent population is acipenser schrencki.
Comparative example 2 the difference is that only that parent population is siberia platform with comparative example 1.
Applicant breeds sturgeon, in 2018 in September, 2016 by the way of Examples 1 to 2 and comparative example 1~2
April in year breeds out fry.Situations such as period, acquisition fertilized eggs and hatching rate, is as follows：
| Group | Fertilized eggs (ten thousand) | Rate of fertilization (%) | Hatch nursery (ten thousand tails) | Hatching rate (%) |
| Embodiment 1 | 18.65 | 91.54 | 13.75 | 73.72 |
| Embodiment 2 | 12.31 | 92.31 | 8.69 | 70.60 |
| Comparative example 1 | 18.61 | 89.86 | 12.27 | 65.93 |
| Comparative example 2 | 12.29 | 90.07 | 7.95 | 64.69 |
As can be known from the above table, the mode mode of reproduction of Examples 1 to 2 is good compared with comparative example 1~2, main reason is that parent population
In breeding process so that the constitution of parent population is more preferable, the egg cell and sperm quality of collection more preferably so that rate of fertilization and hatching
Rate increases.
Above-described is only the embodiment of the present invention, and the common sense such as well known concrete structure and characteristic are not made herein in scheme
Excessive description.It, without departing from the structure of the invention, can be with it should be pointed out that for those skilled in the art
Several modifications and improvements are made, these should also be considered as protection scope of the present invention, these all do not interfere with what the present invention was implemented
Effect and patent practicability.The scope of protection required by this application should be based on the content of the claims, in specification
The records such as specific implementation mode can be used for explaining the content of claim.
Claims (5)
1. a kind of sturgeon mating system, it is characterised in that：Include the following steps：
Step 1: choosing the excellent sturgeon parent population of character；1~2 year before take ovum/essence, parent population is launched by gender to supporting
It grows and carries out a point pond in pond and raise；Parent population injected volume is 4~5kg/ ㎡；Aquaculture pond bottom is in 8~12 ° of inclination angle, the bottom of aquaculture pond
Portion is equipped with the silt of 5~6CM thickness, and filter screen is covered on silt, the cobblestone of 2~3CM thickness is laid on filter screen；In aquaculture pond
Middle dispensing submerged plant and shrimp crab, aquaculture pond 1.8~2m of the depth of water, 7~10mg/L of dissolved oxygen；4~October, holding water temperature 15~
18 DEG C, other months keep 11~14 DEG C of water temperature；Feedstuff feeding amount is to press the 1~1.5% of parent population weight daily；
Step 2: after parent population gonad development reaches the IV phases, drug is carried out to parent population using luteinising hormone-releasing hormo and is hastened parturition, is used
Amount is：1.2~1.5 μ g/kg of milter, 2.5~3 μ g/kg of raun；The drug previous moon to egg cell/seminal fluid collecting of hastening parturition finishes
Period, aquaculture pond use circulating water；
Step 3: the egg cell and sperm to collection are carried out using artificial insemination；Then fertilized eggs are put into incubator and are carried out
Hatching, until seedling incubating is gone out.
2. sturgeon mating system according to claim 1, it is characterised in that：In step 1, the submerged plant is not silk
At least one of with eel grass.
3. sturgeon mating system according to claim 1 or 2, it is characterised in that：In step 1, the shrimp is freshwater shrimp, river
At least one of shrimp, grass shrimp and prawn.
4. sturgeon mating system according to claim 3, it is characterised in that：The drug the specific steps are：To female parent
Fish carries out muscle of back injection using double injection method, and injection dosage is 1 μ g/kg when hastening parturition for the first time, is noted when injecting for the second time
It is 1.5~2 μ g/kg to penetrate dosage, and interval time is 12~14h；Muscle of back injection is carried out using isotope labeled leucine to male parent population,
Injection dosage is 1.2~1.5 μ g/kg.
5. sturgeon mating system according to claim 4, it is characterised in that：In step 3, during incubating oosperm,
Under the premise of ensureing no ovum outflow incubator, fertilized eggs are kept to roll up and down using flow.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201810481217.7A CN108541632A (en) | 2018-05-18 | 2018-05-18 | A kind of sturgeon mating system |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810481217.7A CN108541632A (en) | 2018-05-18 | 2018-05-18 | A kind of sturgeon mating system |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109511581A (en) * | 2018-11-07 | 2019-03-26 | 湖北清江鲟鱼谷特种渔业有限公司 | A kind of sturgeon parent culture method producing caviar |
| CN110178766A (en) * | 2019-07-10 | 2019-08-30 | 新疆伊河鲟业科技股份有限公司 | A kind of artificial breeding method of naked abdomen sturgeon |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109511581A (en) * | 2018-11-07 | 2019-03-26 | 湖北清江鲟鱼谷特种渔业有限公司 | A kind of sturgeon parent culture method producing caviar |
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| CN110178766A (en) * | 2019-07-10 | 2019-08-30 | 新疆伊河鲟业科技股份有限公司 | A kind of artificial breeding method of naked abdomen sturgeon |
| CN110178766B (en) * | 2019-07-10 | 2021-11-09 | 新疆伊河鲟业科技股份有限公司 | Artificial breeding method of Acipenser lapipenser |
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