CN104160986A - Mountain area sturgeon parent breeding and artificial spawning method - Google Patents
Mountain area sturgeon parent breeding and artificial spawning method Download PDFInfo
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- CN104160986A CN104160986A CN201410257306.5A CN201410257306A CN104160986A CN 104160986 A CN104160986 A CN 104160986A CN 201410257306 A CN201410257306 A CN 201410257306A CN 104160986 A CN104160986 A CN 104160986A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses a mountain area sturgeon parent breeding and artificial spawning method. The method comprises the steps that running water breeding is carried out on mountain area sturgeon parents, selection and male and female parent classification are carried out on the sturgeon parents two months before the breeding season, the selected male and female sturgeon parents are bred in different pools with controlled temperature, luteinizing hormone releasing hormone A3 is adopted as oxytocin to facilitate artificial spawning, and male sturgeon parents and female sturgeon parents are respectively and temporarily fed after spawning is finished. The mountain area sturgeon parent breeding and artificial spawning method is easy to operate and low in cost, the fertilization rate of sturgeon reaches 90.48%, 163.2 thousand sturgeon fry are hatched, the hatching rate is 66.55%, and the effect is quite obvious.
Description
Technical field
The present invention relates to a kind of mountain area sturgeon parent culture and induced spawning method, belong to agriculture freshwater aquiculture technical field.
Background technology
1999, Guizhou Province marine Lives Research Academy introduced first sturgeon at our province and carries out development test.For many years, Guizhou Province marine Lives Research Academy has formed the intensive high yielding culture supporting technology of the sturgeon system of applicable our province resources characteristic in sturgeon cultivation technical research, has created 262.7kg/m
2the highest per unit area yield record of national sturgeon cultivation, the fast development of sturgeon cultivation industry has formed the wilderness demand to seed, but due to the factors such as the sturgeon maturing stage is long, technical difficulty is large impact, the artificial propagation of our province sturgeon is for a long time in blank, 5,000,000 tail left and right sturgeon fries, 1,000 ten thousand sturgeon ovum approximately need be introduced from (even external) outside the province every year in the whole province various places, quality, the supply etc. of seed are limited by other people, have greatly limited the fast development of our province mountain area sturgeon cultivation.
Sturgeon artificial breeding technology reaches its maturity.Application number is that the Chinese invention patent of CN201110147872.7 discloses a kind of sturgeon artificial fecundation method, but this invention one is to adopt sign to observe to differentiate female male parent, cannot thoroughly solve female male parent in parent population selection and be difficult to resolution problems; The 2nd, this patent adopts the method that parent is directly proceeded to low temperature environment in parent fish rearing, and the method does not meet the cooling mode under natural conditions, does not illustrate how the rear temperature of low temperature cultivation raises simultaneously; The 3rd, this patent cannot accurately select of the right age parent to carry out artificial breeding.
At present, although the research of sturgeon artificial propagation report is a lot, but for Guizhou mountainous area sturgeon artificial breeding, the one, Guizhou Province is typical Karst Mountain Areas, water resource is abundant, all continues the long period compare with north of china in winter freeze-up and southern Summer High-Temperature Times, has special water resources characteristic, water temperature remains on 15～22 DEG C throughout the year, has therefore caused mountain area sturgeon spy to take different cultivation strategy; The 2nd, the research of artificial breeding for many years learns, indiscriminately imitating sturgeon artificial breeding technology is outside the province impracticable for mountain area sturgeon artificial breeding.
Summary of the invention
The object of the invention is: a kind of mountain area sturgeon parent culture and induced spawning method are provided, thoroughly solve sturgeon parent male and female individuality and be difficult to a difficult problem of differentiating and the age is selected, grope to have set up mountain area sturgeon artificial hypothermia and cultivate flow process and artificial induced spawning strategy, to overcome the deficiencies in the prior art.
Technical scheme of the present invention;
A kind of mountain area sturgeon parent culture and induced spawning method, the method adopts carries out flowing water cultivation to mountain area sturgeon parent, mating season the first two months sturgeon parent is selected and is realized male and female Parent cluster, the trip temperature regulation and control of going forward side by side are cultivated in male and female sturgeon parent point pond after selecting, adopt Luteinizing hormone releasing-hormone A3 (LHRH-A3) as the meticulous artificial induced spawning of ocyodinic, rear female, the male parent of having hastened parturition is supported respectively temporarily, stimulation by running water, collect ripe essence, ovum, adopt dry method and wet method to combine and carry out artificial insemination.
In aforesaid mountain area sturgeon parent culture and induced spawning method, described flowing water is cultivated and is referred to that water exchange capacity is 1.5～2 times/h, water body water temperature remains on 16～22 DEG C, pH value is 7.0～8.0, dissolved oxygen is greater than 6.0mg/L, cultivation density is 5～8kg/m3, and feedstuff feeding amount is that every day is by 1～1.5% of sturgeon parent body weight.
In aforesaid mountain area sturgeon parent culture and induced spawning method, described sturgeon parent selection and sorting technique are for to be implanted into tagging chip at parent population body, set up parent population archives, record the body weight of parent's individuality, the information such as age, at the first two months mating season, pick out physically fit by parent population archives, body surface is bred without the parent population of wound, raun body weight is more than 16kg, milter body weight is more than 12kg, use is got egg apparatus 15cm side before gonopore and is got sexual gland by abdominocentesis and differentiate female male parent and gonad development degree, select female parent's Oocyte maturity to reach the IV phase, ovum ellipse, be dark brown, ovum grain size evenly, the obvious parent population of pigment circle and animal pole, select male parent's testis development to IV or V phase, take out a little spermary tissue and be sprinkled upon the parent population that scatters immediately in water as artificial breeding is used then parent with getting egg apparatus, and by female, male parent population divides pond to cultivate.
In aforesaid mountain area sturgeon parent culture and induced spawning method, parent being carried out to before abdominocentesis is got sexual gland that sturgeon parent is stopped throwing something and feeding 12～20 days.
In aforesaid mountain area sturgeon parent culture and induced spawning method, described temperature adjusting method is that cultivation density ensures at 1～2 kg/m3, water body circulation is 40～60 m3/h, keep oxygen supply, according to the cooling rate of 2 DEG C/d, cultivation water temperature is controlled to below 7 DEG C from normal temperature, after keeping 7 DEG C of following water temperatures to cultivate more than parent population 60d, according to the programming rate of 1 DEG C/d, cultivation water temperature is increased to 14 DEG C, and continue to cultivate after 7d, start parent population to carry out artificial induced spawning.
In aforesaid mountain area sturgeon parent culture and induced spawning method, the concrete steps of described artificial induced spawning adopt double injection method to carry out muscle of back injection to the female parent of sturgeon, while hastening parturition for the first time, injected dose is that 1kg parent injects 1 μ g oxytocins, while injection for the second time, injected dose is that 1kg parent injects 2 μ g oxytocins, and be 12h interval time; Adopt shot method to carry out muscle of back injection to sturgeon male parent, injected dose is that 1kg parent injects 1 μ g oxytocins.
In aforesaid mountain area sturgeon parent culture and induced spawning method, adopting the artificial insemination method that dry method and wet method engage is that maturation essence, ovum are mixed and stir 3～5min, the water that contains seminal fluid is outwelled, with clear water cleaning 3 times, again by fertilized egg containing unsticking 50min in talcous water, incubator fertilized egg putting into agitating function is hatched, when hatching, controlling water temperature is 14～18 DEG C, current are controlled at and can ensure that fish-egg can normally stir, more than when hatching, dissolved oxygen content is controlled at 7.0mg/L, brooding time is 100h left and right.
Owing to having adopted technique scheme, compared with prior art, the method that the present invention adopts flowing water to cultivate is carried out sturgeon parent culture, and by getting by abdominocentesis that sexual gland is differentiated female male parent and gonad development degree and then to sturgeon parent labeled bracketing, form archives, the parent population that adopts spermary tissue to be sprinkled upon to scatter immediately in water is as artificial breeding is used then parent, and the parent who selects is carried out to low temperature cultivation and then carry out artificial breeding, the present invention successfully breeds out first sturgeon fry in Guizhou Province marine Lives Research Academy in proving ground, Huishui, obtain altogether 27.10 ten thousand, fertilized egg, fertilization rate reached 90.48%.Hatch altogether sturgeon fry 16.32 ten thousand tails, incubation rate is 66.55%, and effect is very obvious.
Embodiment;
Below the present invention is further described in detail, but not as any limitation of the invention.
Embodiments of the invention: a kind of mountain area sturgeon parent culture and induced spawning method, the method adopts carries out flowing water cultivation to mountain area sturgeon parent, mating season the first two months sturgeon parent is selected and is realized male and female Parent cluster, the trip temperature regulation and control of going forward side by side are cultivated in male and female sturgeon parent point pond after selecting, adopt Luteinizing hormone releasing-hormone A3 (LHRH-A3) as the meticulous artificial induced spawning of ocyodinic, rear female, the male parent of having hastened parturition is supported respectively temporarily, stimulation by running water, collect ripe essence, ovum, adopt dry method and wet method to combine and carry out artificial insemination.
Concrete grammar is:
One, parent fish rearing;
(1) flowing water is cultivated: adopt the simple and easy flowing water in mountain area efficiently to support sturgeon technology, it is long 20m × wide 6m × dark 1.4m that rectangle is cultivated pond specification, and water temperature remains on 16～22 DEG C, pH 7.0～8.0 throughout the year, dissolved oxygen is greater than 6.0mg/L, 1.5～2 times/h of pond water exchange capacity.
(2) feedstuff feeding: the 6～December of 2011, the reserve parent fish in base, Huishui is carried out to fortification cultivation, the nurturing period sturgeon parent population special feed of throwing something and feeding, cultivating pool cultivated density is 5～8kg/m3, throw something and feed every day by the forage volume of fish body weight 1～1.5%, when first half of the month in the time parent being carried out to the inspection of selected reproduction parent population, stop the parent that throws something and feeds, to prevent because puncture is that damage enteron aisle causes infecting.
Two, parent population is selected;
By breeding archives (being implanted into tagging chip at parent population body), in the reserve parent fish of proving ground, Huishui, select physically fit, without the of the right age parent population hindering, (sturgeon reaches sexually matured asynchronism(-nization) to body surface under normal circumstances, Siberia sturgeon (containing hybridization) needs to cultivate 6～7 years, acipenser schrencki (containing hybridization) needs to cultivate 7～8 years, Da Shi huso sturgeon (containing hybridization) needs to cultivate more than 12 years, require raun body weight more than 16kg, milter body weight is more than 12kg), use is got egg apparatus 15cm side before gonopore and is got sexual gland observation gonad development degree by abdominocentesis, select female parent's Oocyte maturity to reach parent population 17 tails of IV phase, body weight 16.80～21.51kg, all more than 10 ages, chip number is respectively C3C1, 2E11, C3BA, DEDC, BD05, D1E8, E940, C9FD, D48A, DED8, CD2A, C6D1, 2E11, CE31, DED8, E8CA, E8DO, select male parent's testis development to IV or the milter of V phase 7 tails, body weight 12.86～15.20kg/ tail, all, more than 8 ages, chip number is respectively DC35,328B, C976,579C, 680E, 355B, CF3B.Divide pond to cultivate female, male parent population.Three, temperature adjusting;
Female, the male parent that choose are transferred to the cooling tank of automatically controlling temp. controllable equipment, cultivating pool cultivated density is 1～2kg/m3, every group of cooling tank has the compressor of 3 7.5kw, water circulating pump 2.2kw, and water body circulation is 50 m3/h, the oxygen supply of 1.1kw air pump, according to the cooling rate of 2 DEG C/d, cultivation water temperature is controlled to 7 DEG C, keeps 7 DEG C of water temperatures to cultivate after parent population 65d, according to the programming rate of 1 DEG C/d, cultivation water temperature is increased to 18 DEG C, and continue to cultivate after 7d, start to carry out artificial induced spawning.
Four, artificial induced spawning;
Artificial induced spawning adopts Luteinizing hormone releasing-hormone A3 (LHRH-A3) as ocyodinic, adopt double injection method to carry out muscle of back injection to the female parent of sturgeon, while hastening parturition for the first time, injected dose is that 1kg parent injects 1 μ g oxytocins, while injection for the second time, injected dose is that 1kg parent injects 2 μ g oxytocins, and be 12h interval time; Adopt shot method to carry out muscle of back injection to sturgeon male parent, injected dose is that 1kg parent injects 1 μ g oxytocins, supports temporarily respectively stimulation by running water through female, the male parent of injecting the medicine of promoting the sexual maturity.
Five, spawning and hatching;
While having auburn free ovum grain to occur at the bottom of finding raun cultivation pond, adopt sturgeon artificial propagation manually to obtain ripe essence, ovum with device by live egg-fetching technology, fish-egg is put into dry washbasin, add seminal fluid, the artificial insemination that adopts dry method and wet method to engage, after uniform stirring 3～5min, outwells the water that contains seminal fluid, clean 3 times with clear water, then by fertilized egg containing unsticking 50min in talcous water.The good hatching density of bottle formula incubator hatching GPRS finally fertilized egg putting into agitating function, regulates current, removes in time dead ovum, mould ovum.When hatching, controlling water temperature is 14～18 DEG C, and current are controlled at and can ensure that fish-egg can normally stir, and more than when hatching, dissolved oxygen content is controlled at 7.0mg/L, brooding time is 100h left and right.
Six, experimental result;
Through great efforts, in by the end of May, 2012, and first sturgeon fry is successfully bred out in proving ground, Huishui by Guizhou Province marine Lives Research Academy, obtains altogether 27.10 ten thousand, fertilized egg, and fertilization rate reached 90.48% hatches sturgeon fry 16.32 ten thousand tails altogether, and incubation rate is 66.55%.
Claims (7)
1. a mountain area sturgeon parent culture and induced spawning method, it is characterized in that: the method adopts carries out flowing water cultivation to mountain area sturgeon parent, mating season the first two months sturgeon parent is carried out artificial selection and realizes male and female Parent cluster, the trip temperature regulation and control of going forward side by side are cultivated in male and female sturgeon parent point pond after selecting, adopt luteinizing hormone releasing hormone A
3(LHRH-A
3) as the meticulous artificial induced spawning of ocyodinic, rear female, male parent temporary the supporting respectively of having hastened parturition, stimulation by running water, collects ripe essence, ovum, adopts dry method and wet method to combine and carries out artificial insemination.
2. mountain area according to claim 1 sturgeon parent culture and induced spawning method, it is characterized in that: described flowing water is cultivated and referred to that water exchange capacity is 1.5～2 times/h, and water body water temperature remains on 16～22 DEG C, and pH value is 7.0～8.0, dissolved oxygen is greater than 6.0mg/L, and cultivation density is 5～8kg/m
3, feedstuff feeding amount is that every day is by 1～1.5% of sturgeon parent body weight.
3. mountain area according to claim 1 sturgeon parent culture and induced spawning method, it is characterized in that: described sturgeon parent selection and sorting technique are for to be implanted into tagging chip at parent population body, set up parent population archives, record the body weight of parent's individuality, the information such as age, at the first two months mating season, pick out physically fit by parent population archives, body surface is bred without the parent population of wound, raun body weight is more than 16kg, milter body weight is more than 12kg, use is got egg apparatus 15cm side before gonopore and is got sexual gland by abdominocentesis and differentiate female male parent and gonad development degree, select female parent's Oocyte maturity to reach the IV phase, ovum ellipse, be dark brown, ovum grain size evenly, the obvious parent population of pigment circle and animal pole, select male parent's testis development to IV or V phase, take out a little spermary tissue and be sprinkled upon the parent population that scatters immediately in water as artificial breeding is used then parent with getting egg apparatus, and by female, male parent population divides pond to cultivate.
4. mountain area according to claim 3 sturgeon parent culture and induced spawning method, is characterized in that: parent being carried out to before abdominocentesis is got sexual gland that sturgeon parent is stopped throwing something and feeding 12～20 days.
5. mountain area according to claim 1 sturgeon parent culture and induced spawning method, is characterized in that: described temperature adjusting method is that cultivation density ensures at 1～2 kg/m
3water body circulation is 40～60 m3/h, keep oxygen supply, according to the cooling rate of 2 DEG C/d, cultivation water temperature is controlled to below 7 DEG C from normal temperature, after keeping 7 DEG C of following water temperatures to cultivate more than parent population 60d, according to the programming rate of 1 DEG C/d, cultivation water temperature is increased to 14 DEG C, and continues to cultivate after 7d, start parent population to carry out artificial induced spawning.
6. mountain area according to claim 1 sturgeon parent culture and induced spawning method, it is characterized in that: the concrete steps of described artificial induced spawning adopt double injection method to carry out muscle of back injection to the female parent of sturgeon, while hastening parturition for the first time, injected dose is that 1kg parent injects 1 μ g oxytocins, while injection for the second time, injected dose is that 1kg parent injects 2 μ g oxytocins, and be 12h interval time; Adopt shot method to carry out muscle of back injection to sturgeon male parent, injected dose is that 1kg parent injects 1 μ g oxytocins.
7. mountain area according to claim 1 sturgeon parent culture and induced spawning method, it is characterized in that: adopting the artificial insemination method that dry method and wet method engage is by maturation essence, ovum mixes and stirs 3～5min, the water that contains seminal fluid is outwelled, with clear water cleaning 3 times, again by fertilized egg containing unsticking 50min in talcous water, incubator fertilized egg putting into agitating function is hatched, when hatching, controlling water temperature is 14～18 DEG C, current are controlled at and can ensure that fish-egg can normally stir, more than when hatching, dissolved oxygen content is controlled at 7.0mg/L, brooding time is 100h left and right.
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105028269A (en) * | 2015-06-25 | 2015-11-11 | 遵义绥阳县芙蓉江鲟鱼繁养有限公司 | Seedling raising method for sturgeon |
| CN105210950A (en) * | 2015-10-27 | 2016-01-06 | 贵阳市金阳新区谷纳冷水养殖有限公司 | The artificial culturing method of a kind of sturgeon |
| CN105900879A (en) * | 2016-04-25 | 2016-08-31 | 石家庄市环境监测中心 | Efficient oryzias latipe breeding and hatching method |
| CN106069899A (en) * | 2016-06-15 | 2016-11-09 | 中国水产科学研究院长江水产研究所 | Artificial regulatory realizes the method that acipenser dabryanus natural mating is laid eggs |
| CN106719170A (en) * | 2016-12-30 | 2017-05-31 | 贵州锦润水产品有限责任公司 | A kind of sturgeon hatching method |
| CN107047401A (en) * | 2017-04-28 | 2017-08-18 | 贵州省水产研究所 | A kind of mountain area loach parent culture and induced spawning method and hatching system |
| CN107278990A (en) * | 2017-08-04 | 2017-10-24 | 安徽省泾县丰瑞水产科技有限公司 | A kind of sturgeon artificial advance propagation method |
| CN107549063A (en) * | 2017-09-28 | 2018-01-09 | 临汾市尧都区云盛水产养殖有限公司 | A kind of sturgeon artificial propagation tank and propagation method |
| CN107581107A (en) * | 2017-09-28 | 2018-01-16 | 临汾市尧都区云盛水产养殖有限公司 | A kind of sturgeon propagation method |
| CN107624684A (en) * | 2017-09-28 | 2018-01-26 | 临汾市尧都区云盛水产养殖有限公司 | A kind of female sturgeon ovum artificial collection method |
| CN108251356A (en) * | 2017-12-29 | 2018-07-06 | 中山大学 | A kind of fish-egg cleaning solution of anti-fish nervous necrosis virus |
| CN108541632A (en) * | 2018-05-18 | 2018-09-18 | 贵州龙源冷水渔业有限公司 | A kind of sturgeon mating system |
| CN109511581A (en) * | 2018-11-07 | 2019-03-26 | 湖北清江鲟鱼谷特种渔业有限公司 | A kind of sturgeon parent culture method producing caviar |
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| CN105900879A (en) * | 2016-04-25 | 2016-08-31 | 石家庄市环境监测中心 | Efficient oryzias latipe breeding and hatching method |
| CN105900879B (en) * | 2016-04-25 | 2018-08-21 | 石家庄市环境监测中心 | A kind of medaka efficiently breeding and hatching method |
| CN106069899A (en) * | 2016-06-15 | 2016-11-09 | 中国水产科学研究院长江水产研究所 | Artificial regulatory realizes the method that acipenser dabryanus natural mating is laid eggs |
| CN106719170A (en) * | 2016-12-30 | 2017-05-31 | 贵州锦润水产品有限责任公司 | A kind of sturgeon hatching method |
| CN107047401A (en) * | 2017-04-28 | 2017-08-18 | 贵州省水产研究所 | A kind of mountain area loach parent culture and induced spawning method and hatching system |
| CN107278990A (en) * | 2017-08-04 | 2017-10-24 | 安徽省泾县丰瑞水产科技有限公司 | A kind of sturgeon artificial advance propagation method |
| CN107581107A (en) * | 2017-09-28 | 2018-01-16 | 临汾市尧都区云盛水产养殖有限公司 | A kind of sturgeon propagation method |
| CN107624684A (en) * | 2017-09-28 | 2018-01-26 | 临汾市尧都区云盛水产养殖有限公司 | A kind of female sturgeon ovum artificial collection method |
| CN107549063A (en) * | 2017-09-28 | 2018-01-09 | 临汾市尧都区云盛水产养殖有限公司 | A kind of sturgeon artificial propagation tank and propagation method |
| CN108251356A (en) * | 2017-12-29 | 2018-07-06 | 中山大学 | A kind of fish-egg cleaning solution of anti-fish nervous necrosis virus |
| CN108251356B (en) * | 2017-12-29 | 2021-06-22 | 中山大学 | Fish egg cleaning solution for resisting fish nervous necrosis virus |
| CN108541632A (en) * | 2018-05-18 | 2018-09-18 | 贵州龙源冷水渔业有限公司 | A kind of sturgeon mating system |
| CN109511581A (en) * | 2018-11-07 | 2019-03-26 | 湖北清江鲟鱼谷特种渔业有限公司 | A kind of sturgeon parent culture method producing caviar |
| CN109511581B (en) * | 2018-11-07 | 2021-08-24 | 湖北清江鲟鱼谷特种渔业有限公司 | Sturgeon parent breeding method for producing caviar |
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