CN107278990A - A kind of sturgeon artificial advance propagation method - Google Patents

A kind of sturgeon artificial advance propagation method Download PDF

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Publication number
CN107278990A
CN107278990A CN201710659980.XA CN201710659980A CN107278990A CN 107278990 A CN107278990 A CN 107278990A CN 201710659980 A CN201710659980 A CN 201710659980A CN 107278990 A CN107278990 A CN 107278990A
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parts
sturgeon
parent population
artificial
water temperature
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周洵
章星明
芮金兵
沈蓓杰
俞爱萍
孙德祥
李正荣
许世富
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Jingxian County Anhui Feng Feng Aquatic Science And Technology Co Ltd
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Jingxian County Anhui Feng Feng Aquatic Science And Technology Co Ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
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    • A01K61/10—Culture of aquatic animals of fish
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00—Culture of aquatic animals
    • A01K61/10—Culture of aquatic animals of fish
    • A01K61/17—Hatching, e.g. incubators
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A23K10/24—Animal feeding-stuffs from material of animal origin from blood
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/174—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

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Abstract

The invention discloses a kind of sturgeon artificial advance propagation method, comprise the following steps：Parent population selects：The parent population of physique healthy and strong, body surface without wound is selected at the beginning of 2 months from the parent population of reinforced cultivating to be put into spawning inducing pool；Artificial stimulation：Parent population, which enters, to be started interval after spawning inducing pool and feeds breeding feed, and breeding is stopped eating for preceding 15 days, and breeding starts the water temperature of raising spawning inducing pool for first 10 days, and the water temperature of spawning inducing pool keeps water temperature after rising to 15 ± 1 DEG C；During the water temperature of spawning inducing pool is improved, 24h opens microcirculation water and carries out current stimulation, while carrying out illumination, circulation of sheltering from heat or light stimulates；Artificial induced spawning：Water temperature is kept after 2 weeks, using luteinizing hormone releasing hormone A3As ocyodinic, the male and female parent population after hastening parturition separates, and continuing current stimulates；Artificial insemination：When finding that brown ovum grain occurs in sturgeon raun spawning inducing pool bottom, start artificial insemination.

Description

A kind of sturgeon artificial advance propagation method
Technical field
The present invention relates to artificial aquatic products reproduction technique field, more particularly to a kind of sturgeon artificial advance propagation method.
Background technology
Most of artificial propagation sturgeons are selected in annual June and December, and this is also the cycle of sturgeon natural propagation, now sturgeon Seedling is largely listed, and causes sturgeon seed price relatively low.Lack one kind at present to breed in advance by a series of stimulating methods in spring Sturgeon seed, can make sturgeon fry kind price be far above the seed of sturgeon artificial propagation under normal cycle.
The content of the invention
The technical problem existed based on background technology, the present invention proposes a kind of sturgeon artificial advance propagation method, uses The mode that breeding feed, water temperature are stimulated, current are stimulated and light stimulation cooperates, promotes sturgeon parent population gonad development, improves The concentration of sex hormone in sturgeon parent population body, so as to promote sturgeon parent population breeding period to shift to an earlier date significantly, improves the fertilization of sturgeon parent population Rate.
A kind of sturgeon artificial advance propagation method proposed by the present invention, comprises the following steps：
A, parent population selection：From the parent population of reinforced cultivating the parent population of physique healthy and strong, body surface without wound is selected at the beginning of 2 months be put into urge Produce in pond；
B, artificial stimulation：Parent population, which enters, to be started interval after spawning inducing pool and feeds breeding feed, and breeding is stopped eating for first 15 days, before breeding Start within 10 days the water temperature of raising spawning inducing pool, the water temperature of spawning inducing pool keeps water temperature after rising to 15 ± 1 DEG C；Improving the water temperature of spawning inducing pool During, 24h opens microcirculation water and carries out current stimulation, while carrying out illumination, circulation of sheltering from heat or light stimulates；
C, artificial induced spawning：Water temperature is kept after 2 weeks, using luteinizing hormone releasing hormone A3It is female after hastening parturition as ocyodinic Male parent population separates, and continuing current stimulates；
D, artificial insemination：When finding that brown ovum grain occurs in sturgeon raun spawning inducing pool bottom, start artificial insemination.
Preferably, in step A, the female-male proportion of parent population is 1：1-2, wherein raun age are for 8 more than age, and body weight is 15kg More than, the milter age, body weight was more than 14kg for 7 more than age.
When water temperature is less than 10 DEG C, parent population is selected in progress.It is parent population gonadal maturation degree to select foundation：Raun parent Ovum it is oval, dark brown, ovum grain it is uniform in size, maturation of ovum degree reached for 4 phases；The sexual gland of milter parent is full, extrudes milter , there is off-white color liquefaction liquid semen in the belly of parent.
Preferably, in step B, the raw material of breeding feed includes by weight：35-65 parts of millet powder, 10-18 parts of wheat bran, 4-12 parts of alfalfa meal, 4-8 parts of expanding blood meal, 4-16 parts of soyabean protein powder, 2-8 parts of yellow meal worm powder, 1-2 parts of montmorillonite, chlorination courage 0.01-0.06 parts of alkali, 0.02-0.08 parts of Rhizopus oryzae, 1-2 parts of grape-kernel oil, 1-2 parts of barrenwort, 1-2 parts of cynomorium songaricum, thizoma curculiginis 2-4 Part, 4-8 parts of the fruit of Chinese wolfberry, 1-3 parts of cyclodextrin complexes.
Preferably, in step B, cyclodextrin complexes are prepared using following technique：Honey is heated up, vitamin E is added, gathers Ethylene glycol is stirred, and adds cyclodextrin, and heating stirring, freeze-drying, vacuum drying obtains cyclodextrin complexes.
Preferably, in step B, cyclodextrin complexes are prepared using following technique：Honey is warming up to 34-45 DEG C, addition Vitamin E, polyethylene glycol stirring 5-15min, mixing speed is 1400-1600r/min, adds cyclodextrin, is warming up to 60-70 DEG C 30-50min is stirred, freeze-drying, 40-50 DEG C of vacuum drying obtains cyclodextrin complexes.
Preferably, in step B, cyclodextrin complexes are prepared using following technique：20-40 parts of honey are heated up by weight To 34-45 DEG C, 1-3 parts of vitamin Es, 2-4 parts of polyethylene glycol stirring 5-15min are added, mixing speed is 1400-1600r/min, 1-2 parts of cyclodextrin are added, 60-70 DEG C of stirring 30-50min is warming up to, freeze-drying, 40-50 DEG C of vacuum drying obtains cyclodextrin Compound.
Preferably, in step B, it is as follows that interval feeds breeding feed concrete operations：Feed 2-3 days, stop eating 1 day.
Preferably, in step B, water temperature heating rate is 0.4-0.6 DEG C/day, and light application time is 9-11h, and the time of sheltering from heat or light is 1-3h。
Preferably, in step C, sturgeon milter is using 1 muscle of back injection, and dosage is 0.8-1.2 μ g/kg；Sturgeon Raun is using 2 muscle of back injections, and first time dosage is 0.8-1.2 μ g/kg, is spaced after 11-13h, 1.8- is injected again 2.2μg/kg。
Preferably, in step D, the concrete operations of artificial insemination are as follows：Mature brood fish has been caught put on the skin it is dry after, extrude sturgeon with hand Fish parent fish belly, obtains ripe seminal fluid or mature egg grain；By ripe seminal fluid and mature egg grain mixing, it is placed in clear water and persistently stirs 3-5min, is then filtered, and clear water cleans 4-5 times and obtains embryonated egg.
The present invention is stimulated by using breeding feed, water temperature, current are stimulated and the mode of light stimulation, promotes sturgeon parent population Gonad development, improves the concentration of sex hormone in sturgeon parent population body；Coordinate millet powder, wheat using cyclodextrin complexes in breeding feed Bran, alfalfa meal, expanding blood meal, soyabean protein powder, yellow meal worm powder, albumen, fat and fiber needed for not only can providing sturgeon parent population, And energy is balanced with albumen, green and healthy are cost-effective；And in cyclodextrin complexes, polyethylene glycol is compounded with cyclodextrin, than The higher dispersiveness of former cyclodextrin, it is extremely strong to honey and vitamin encapsulation, and also average grain diameter is small, it is easier to digests and assimilates, Not only it is of high nutritive value, and by high-speed stirred, freezing vacuum processing, and by certain heat treatment, dispersing uniformity is extremely strong, The original local flavor of honey and quality can be ensured, solubility is good, digestion and absorption suitable for sturgeon parent population；Choline Chloride, Rhizopus oryzae, Grape-kernel oil mating reaction, can effectively improve sturgeon parent population immunity, reduce case fatality rate；And barrenwort, cynomorium songaricum, thizoma curculiginis, matrimony vine Son cooperates, and can promote sturgeon parent population gonad development with tonifying kidney and strengthening yang strengthening the essence, subsequent artefacts can be coordinated to stimulate and manually urge Production, further improves artificial insemination success rate.
The present invention is again using injection luteinizing hormone releasing hormone A3, further promote milter sperm to produce and ripe, promote Raun ovarian follicle secretes estrogen, makes follicle maturity, ovulation and corpus luteum generation, so as to promote sturgeon parent population breeding period to shift to an earlier date significantly； Using artificial insemination, ovum is contacted with seminal fluid directly mixing, substantially increase the rate of fertilization of sturgeon parent population.
Embodiment
Below, technical scheme is described in detail by specific embodiment.
Embodiment 1
A kind of sturgeon artificial advance propagation method, comprises the following steps：
A, parent population selection：From the parent population of reinforced cultivating the parent population of physique healthy and strong, body surface without wound is selected at the beginning of 2 months be put into urge Produce in pond；
B, artificial stimulation：Parent population, which enters, to be started interval after spawning inducing pool and feeds breeding feed, and breeding is stopped eating for first 15 days, before breeding Start within 10 days the water temperature of raising spawning inducing pool, the water temperature of spawning inducing pool keeps water temperature after rising to 15 ± 1 DEG C；Improving the water temperature of spawning inducing pool During, 24h opens microcirculation water and carries out current stimulation, while carrying out illumination, circulation of sheltering from heat or light stimulates；
C, artificial induced spawning：Water temperature is kept after 2 weeks, using luteinizing hormone releasing hormone A3It is female after hastening parturition as ocyodinic Male parent population separates, and continuing current stimulates；
D, artificial insemination：When finding that brown ovum grain occurs in sturgeon raun spawning inducing pool bottom, start artificial insemination.
Embodiment 2
A kind of sturgeon artificial advance propagation method, comprises the following steps：
A, parent population selection：From the parent population of reinforced cultivating the parent population of physique healthy and strong, body surface without wound is selected at the beginning of 2 months be put into urge Produce in pond, the female-male proportion of parent population is 1：1, the wherein raun age is that 8 more than age, body weight is more than 15kg, and the milter age was 7 ages More than, body weight is more than 14kg；
B, artificial stimulation：Parent population starts interval and feeds breeding feed after entering spawning inducing pool, often feeds after 2-3 days, stops eating 1 My god；Breeding is stopped eating for first 15 days, and breeding starts to improve the water temperature of spawning inducing pool for first 10 days, and water temperature heating rate is 0.6 DEG C/day, illumination Time is 9h, and the time of sheltering from heat or light, the water temperature of spawning inducing pool kept water temperature after rising to 14 DEG C for 3h；Improving the water temperature process of spawning inducing pool In, 24h opens microcirculation water and carries out current stimulation, while carrying out illumination, circulation of sheltering from heat or light stimulates；
The raw material of breeding feed includes by weight：35 parts of millet powder, 18 parts of wheat bran, 4 parts of alfalfa meal, 8 parts of expanding blood meal, 4 parts of soyabean protein powder, 8 parts of yellow meal worm powder, 1 part of montmorillonite, 0.06 part of Choline Chloride, 0.02 part of Rhizopus oryzae, 2 parts of grape-kernel oil, 1 part of barrenwort, 2 parts of cynomorium songaricum, 2 parts of thizoma curculiginis, 8 parts of the fruit of Chinese wolfberry, 1 part of cyclodextrin complexes；
Cyclodextrin complexes are prepared using following technique：Honey is warming up to 40 DEG C, vitamin E, polyethylene glycol is added and stirs 10min is mixed, mixing speed is 1500r/min, add cyclodextrin, be warming up to 65 DEG C of stirring 40min, freeze-drying, 45 DEG C of vacuum It is dried to obtain cyclodextrin complexes；
C, artificial induced spawning：Water temperature is kept after 2 weeks, using luteinizing hormone releasing hormone A3As ocyodinic, sturgeon milter is adopted With 1 muscle of back injection, dosage is 0.8 μ g/kg；Sturgeon raun uses 2 muscle of back injections, and first time dosage is After 1.2 μ g/kg, interval 11h, 2.2 μ g/kg are injected again；Male and female parent population after hastening parturition separates, and continuing current stimulates；
D, artificial insemination：When finding that brown ovum grain occurs in sturgeon raun spawning inducing pool bottom, mature brood fish is caught and has put dry on the skin Afterwards, sturgeon parent population belly is extruded with hand, obtains ripe seminal fluid or mature egg grain；By ripe seminal fluid and mature egg grain mixing, it is placed in 3min is persistently stirred in clear water, is then filtered, clear water cleans 5 times and obtains embryonated egg.
Embodiment 3
A kind of sturgeon artificial advance propagation method, comprises the following steps：
A, parent population selection：From the parent population of reinforced cultivating the parent population of physique healthy and strong, body surface without wound is selected at the beginning of 2 months be put into urge Produce in pond, the female-male proportion of parent population is 1：2, the wherein raun age is that 8 more than age, body weight is more than 15kg, and the milter age was 7 ages More than, body weight is more than 14kg；
B, artificial stimulation：Parent population starts interval and feeds breeding feed after entering spawning inducing pool, often feeds after 2-3 days, stops eating 1 My god；Breeding is stopped eating for first 15 days, and breeding starts to improve the water temperature of spawning inducing pool for first 10 days, and water temperature heating rate is 0.4 DEG C/day, illumination Time is 11h, and the time of sheltering from heat or light, the water temperature of spawning inducing pool kept water temperature after rising to 16 DEG C for 1h；Improving the water temperature process of spawning inducing pool In, 24h opens microcirculation water and carries out current stimulation, while carrying out illumination, circulation of sheltering from heat or light stimulates；
The raw material of breeding feed includes by weight：50 parts of millet powder, 14 parts of wheat bran, 8 parts of alfalfa meal, 6 parts of expanding blood meal, 10 parts of soyabean protein powder, 5 parts of yellow meal worm powder, 1.5 parts of montmorillonite, 0.035 part of Choline Chloride, 0.05 part of Rhizopus oryzae, grape-kernel oil 1.5 parts, 1.5 parts of barrenwort, 1.5 parts of cynomorium songaricum, 3 parts of thizoma curculiginis, 6 parts of the fruit of Chinese wolfberry, 2 parts of cyclodextrin complexes；
Cyclodextrin complexes are prepared using following technique：20 parts of honey are warming up to 45 DEG C by weight, 1 part of dimension life is added Plain E, 4 parts of polyethylene glycol stirring 5min, mixing speed is 1600r/min, adds 1 part of cyclodextrin, is warming up to 70 DEG C of stirrings 30min, freeze-drying, 50 DEG C of vacuum drying obtain cyclodextrin complexes；
C, artificial induced spawning：Water temperature is kept after 2 weeks, using luteinizing hormone releasing hormone A3As ocyodinic, sturgeon milter is adopted With 1 muscle of back injection, dosage is 1.2 μ g/kg；Sturgeon raun uses 2 muscle of back injections, and first time dosage is After 0.8 μ g/kg, interval 13h, 1.8 μ g/kg are injected again；Male and female parent population after hastening parturition separates, and continuing current stimulates；
D, artificial insemination：When finding that brown ovum grain occurs in sturgeon raun spawning inducing pool bottom, mature brood fish is caught and has put dry on the skin Afterwards, sturgeon parent population belly is extruded with hand, obtains ripe seminal fluid or mature egg grain；By ripe seminal fluid and mature egg grain mixing, it is placed in 5min is persistently stirred in clear water, is then filtered, clear water cleans 4 times and obtains embryonated egg.
Embodiment 4
A kind of sturgeon artificial advance propagation method, comprises the following steps：
A, parent population selection：From the parent population of reinforced cultivating the parent population of physique healthy and strong, body surface without wound is selected at the beginning of 2 months be put into urge Produce in pond, the female-male proportion of parent population is 1：The raun age is that 8 more than age, body weight is more than 15kg in 1.5, and the milter age was 7 ages More than, body weight is more than 14kg；
B, artificial stimulation：Parent population starts interval and feeds breeding feed after entering spawning inducing pool, often feeds after 2-3 days, stops eating 1 My god；Breeding is stopped eating for first 15 days, and breeding starts to improve the water temperature of spawning inducing pool for first 10 days, and water temperature heating rate is 0.5/ day, during illumination Between be 10 times of sheltering from heat or light be 2, the water temperature of spawning inducing pool keeps water temperature after rising to 15 DEG C；During the water temperature of spawning inducing pool is improved, 24h Open microcirculation water and carry out current stimulation, while carrying out illumination, circulation of sheltering from heat or light stimulates；
The raw material of breeding feed includes by weight：65 parts of millet powder, 10 parts of wheat bran, 12 parts of alfalfa meal, expanding blood meal 4 Part, 16 parts of soyabean protein powder, 2 parts of yellow meal worm powder, 2 parts of montmorillonite, 0.01 part of Choline Chloride, 0.08 part of Rhizopus oryzae, grape-kernel oil 1 Part, 2 parts of barrenwort, 1 part of cynomorium songaricum, 4 parts of thizoma curculiginis, 4 parts of the fruit of Chinese wolfberry, 3 parts of cyclodextrin complexes；
Cyclodextrin complexes are prepared using following technique：40 parts of honey are warming up to 34 DEG C by weight, 3 parts of dimension lifes are added Plain E, 2 parts of polyethylene glycol stirring 15min, mixing speed is 1400r/min, adds 2 parts of cyclodextrin, is warming up to 60 DEG C of stirrings 50min, freeze-drying, 40 DEG C of vacuum drying obtain cyclodextrin complexes；
C, artificial induced spawning：Water temperature is kept after 2 weeks, using luteinizing hormone releasing hormone A3As ocyodinic, sturgeon milter is adopted With 1 muscle of back injection, dosage is 1g/kg；Sturgeon raun is using 2 muscle of back injections, and first time dosage is 1g/ After kg, interval 11-13h, 2g/kg is injected again；Male and female parent population after hastening parturition separates, and continuing current stimulates；
D, artificial insemination：When finding that brown ovum grain occurs in sturgeon raun spawning inducing pool bottom, mature brood fish is caught and has put dry on the skin Afterwards, sturgeon parent population belly is extruded with hand, obtains ripe seminal fluid or mature egg grain；By ripe seminal fluid and mature egg grain mixing, it is placed in 4min is persistently stirred in clear water, is then filtered, clear water cleans 5 times and obtains embryonated egg.
Using the method for the embodiment of the present invention 4, sturgeon breeding period is set to have done sth. in advance 3-4 months, and the fertilization rate reached of sturgeon 90%.
The foregoing is only a preferred embodiment of the present invention, but protection scope of the present invention be not limited thereto, Any one skilled in the art the invention discloses technical scope in, technique according to the invention scheme and its Inventive concept is subject to equivalent substitution or change, should all be included within the scope of the present invention.

Claims (10)

1. a kind of sturgeon artificial advance propagation method, it is characterised in that comprise the following steps：
A, parent population selection：The parent population of physique healthy and strong, body surface without wound is selected from the parent population of reinforced cultivating be put into spawning inducing pool at the beginning of 2 months In；
B, artificial stimulation：Parent population, which enters, to be started interval after spawning inducing pool and feeds breeding feed, and breeding is stopped eating for preceding 15 days, first 10 days of breeding Start the water temperature of raising spawning inducing pool, the water temperature of spawning inducing pool keeps water temperature after rising to 15 ± 1 DEG C；Improving the water temperature process of spawning inducing pool In, 24h opens microcirculation water and carries out current stimulation, while carrying out illumination, circulation of sheltering from heat or light stimulates；
C, artificial induced spawning：Water temperature is kept after 2 weeks, using luteinizing hormone releasing hormone A3As ocyodinic, the male and female parent population after hastening parturition Separate, continuing current stimulates；
D, artificial insemination：When finding that brown ovum grain occurs in sturgeon raun spawning inducing pool bottom, start artificial insemination.
2. sturgeon artificial advance propagation method according to claim 1, it is characterised in that in step A, the female-male proportion of parent population For 1：1-2, wherein raun age be 8 more than age, and body weight is more than 15kg, and the milter age is 7 more than age, body weight be 14kg with On.
3. sturgeon artificial advance propagation method according to claim 1 or claim 2, it is characterised in that in step B, breeding feed Raw material includes by weight：35-65 parts of millet powder, 10-18 parts of wheat bran, 4-12 parts of alfalfa meal, 4-8 parts of expanding blood meal, soybean egg 4-16 parts of white powder, 2-8 parts of yellow meal worm powder, 1-2 parts of montmorillonite, 0.01-0.06 parts of Choline Chloride, 0.02-0.08 parts of Rhizopus oryzae, Portugal 1-2 parts of grape seed oil, 1-2 parts of barrenwort, 1-2 parts of cynomorium songaricum, 2-4 parts of thizoma curculiginis, 4-8 parts of the fruit of Chinese wolfberry, 1-3 parts of cyclodextrin complexes.
4. sturgeon artificial advance propagation method according to claim 3, it is characterised in that in step B, cyclodextrin complexes are adopted Prepared with following technique：Honey is heated up, vitamin E, polyethylene glycol stirring is added, cyclodextrin is added, heating stirring, freezing is dry Dry, vacuum drying obtains cyclodextrin complexes.
5. the sturgeon artificial advance propagation method according to claim 3 or 2, it is characterised in that in step B, cyclodextrin is combined Thing is prepared using following technique：Honey is warming up to 34-45 DEG C, vitamin E, polyethylene glycol stirring 5-15min, stirring speed is added Spend for 1400-1600r/min, add cyclodextrin, be warming up to 60-70 DEG C of stirring 30-50min, freeze-drying, 40-50 DEG C of vacuum It is dried to obtain cyclodextrin complexes.
6. according to any one of the claim 3-5 sturgeon artificial advance propagation methods, it is characterised in that in step B, cyclodextrin Compound is prepared using following technique：20-40 parts of honey are warming up to 34-45 DEG C by weight, 1-3 parts of vitamin Es, 2- are added 4 parts of polyethylene glycol stir 5-15min, and mixing speed is 1400-1600r/min, add 1-2 parts of cyclodextrin, are warming up to 60-70 DEG C 30-50min is stirred, freeze-drying, 40-50 DEG C of vacuum drying obtains cyclodextrin complexes.
7. according to any one of the claim 1-6 sturgeon artificial advance propagation methods, it is characterised in that in step B, interval is thrown Feed breeding feed concrete operations as follows：Feed 2-3 days, stop eating 1 day.
8. according to any one of the claim 1-7 sturgeon artificial advance propagation methods, it is characterised in that in step B, water temperature liter Warm speed is 0.4-0.6 DEG C/day, and light application time is 9-11h, and the time of sheltering from heat or light is 1-3h.
9. according to any one of the claim 1-8 sturgeon artificial advance propagation methods, it is characterised in that in step C, sturgeon is male Fish is using 1 muscle of back injection, and dosage is 0.8-1.2 μ g/kg；Sturgeon raun is using 2 muscle of back injections, first Secondary dosage is 0.8-1.2 μ g/kg, is spaced after 11-13h, and 1.8-2.2 μ g/kg are injected again.
10. according to any one of the claim 1-9 sturgeon artificial advance propagation methods, it is characterised in that in step D, manually The concrete operations of insemination are as follows：Mature brood fish has been caught put on the skin it is dry after, extrude sturgeon parent population belly with hand, obtain ripe seminal fluid or into Ripe ovum grain；By ripe seminal fluid and mature egg grain mixing, it is placed in clear water and persistently stirs, then filter, cleaning obtains embryonated egg.
CN201710659980.XA 2017-08-04 2017-08-04 A kind of sturgeon artificial advance propagation method Pending CN107278990A (en)

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CN108925478A (en) * 2018-09-11 2018-12-04 安徽涂远农业有限公司 A kind of cultural method improving freshwater crayfish survival rate
CN109511581A (en) * 2018-11-07 2019-03-26 湖北清江鲟鱼谷特种渔业有限公司 A kind of sturgeon parent culture method producing caviar

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CN102165929A (en) * 2011-06-02 2011-08-31 广州市先步农业发展有限公司 Artificial propagation method for sturgeon
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CN104160986A (en) * 2014-06-11 2014-11-26 贵州省水产研究所 Mountain area sturgeon parent breeding and artificial spawning method
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Application publication date: 20171024