CN105766731A - Low-cost propagation method of bass - Google Patents
Low-cost propagation method of bass Download PDFInfo
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- CN105766731A CN105766731A CN201610192284.8A CN201610192284A CN105766731A CN 105766731 A CN105766731 A CN 105766731A CN 201610192284 A CN201610192284 A CN 201610192284A CN 105766731 A CN105766731 A CN 105766731A
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- 238000000034 method Methods 0.000 title claims abstract description 23
- 230000001568 sexual effect Effects 0.000 claims abstract description 9
- 230000012447 hatching Effects 0.000 claims abstract description 7
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- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 6
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- 238000009395 breeding Methods 0.000 claims description 12
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- XUIIKFGFIJCVMT-GFCCVEGCSA-N D-thyroxine Chemical compound IC1=CC(C[C@@H](N)C(O)=O)=CC(I)=C1OC1=CC(I)=C(O)C(I)=C1 XUIIKFGFIJCVMT-GFCCVEGCSA-N 0.000 claims description 6
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- 238000002360 preparation method Methods 0.000 claims description 2
- 230000008901 benefit Effects 0.000 abstract description 3
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- 238000005728 strengthening Methods 0.000 description 4
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- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 1
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- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000007102 metabolic function Effects 0.000 description 1
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- 239000011664 nicotinic acid Substances 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
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- 229910052710 silicon Inorganic materials 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 235000010374 vitamin B1 Nutrition 0.000 description 1
- 239000011691 vitamin B1 Substances 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Feed For Specific Animals (AREA)
- Farming Of Fish And Shellfish (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a low-cost propagation method of bass. The method comprises steps as follows: parent bass culture, parent bass selection, artificial spawning induction and fry hatching. Compared with the prior art, the method has the advantages as follows: at the intensified culture stage of parent bass, male and female parents of the bass are cultured respectively, different management is performed according to physiological characteristics of the male and female parents, so that the sexual maturity period of the parents is advanced, the gonad index of male bass is optimized, the ovum quality and the ovulation amount are increased, the fertilization rate and the hatching rate are increased, the labor intensity and the propagation cost for artificial propagation are reduced, and large-scale popularization of artificial propagation of the bass is facilitated.
Description
Technical field
The invention belongs to technical field of aquaculture, be specifically related to the low cost propagation method of a kind of Lateolabrax japonicus (Cuvier et Va-lenciennes) (Lateolabracis).
Background technology
Lateolabrax japonicus (Cuvier et Va-lenciennes) (Lateolabracis) is subordinate to Perciformes, normal Lateolabrax japonicus (Cuvier et Va-lenciennes) (Lateolabracis) subfamily, eurysaline fishes, China coast all has distribution, aboundresources, there is growth fast, wide adaptability, premunition is strong, the features such as delicious meat, deeply popular to people, but the peak period of breeding of Lateolabrax japonicus (Cuvier et Va-lenciennes) (Lateolabracis) is the 3-5 month, initial stage in this stage, owing to sky temperature is unstable, breeding of Lateolabrax japonicus (Cuvier et Va-lenciennes) (Lateolabracis) is often made to affect, in order to ensure carrying out ahead of time of Lateolabrax japonicus (Cuvier et Va-lenciennes) (Lateolabracis) artificial propagation, breeding potential is improved usually by changing aquaculture water temperature, ectogenetic cost can be considerably increased so again, therefore, need the propagation method of a kind of advantage of lower cost.
Summary of the invention
It is an object of the invention to for the problem that existing strengthening aquaculture cost is high and fertility rate and hatchability is low, it is provided that the low cost propagation method of a kind of Lateolabrax japonicus (Cuvier et Va-lenciennes) (Lateolabracis).
The present invention is achieved by the following technical solutions: the low cost propagation method of a kind of Lateolabrax japonicus (Cuvier et Va-lenciennes) (Lateolabracis), comprises the following steps:
(1) parent fish is cultivated: after the beginning of spring, sexually matured male and female Lateolabrax japonicus (Cuvier et Va-lenciennes) (Lateolabracis) is cultivated respectively, male Lateolabrax japonicus (Cuvier et Va-lenciennes) (Lateolabracis) presses 5-6 tail/cultivation in pond, greenhouse, starts aerator, and pond, the greenhouse depth of water is 0.5 meter, salinity is 2.8-3.2%, pH value is 7.2-8, every water of 4 Tianchong, maintains water temperature at and is stepped up between 8-18 DEG C, regularly throw something and feed agreeable to the taste living bait fish, it is ensured that bait is sufficient;In breeding first half of the month, bath in every two days once, was filled with ozone 1 hour every 3 days by ozonator, keeps water temperature to be 16-20 DEG C, reduces the feeding volume 10% of living bait fish, adopted the made granule of leaf lettuce to replace;
Raun adopts net cage to cultivate, the deep 3m of net cage, square is a dried up 20cm, salinity is 2.3-2.6%, and pH value is 6.5-7.2, breeding density be 6-8 tail/, maintain water temperature at and be stepped up between 10-20 DEG C, a bait of throwing something and feeding every day, described bait is live bait, including the forage fiss 70% and the bait shrimp 30% that are equivalent to Lateolabrax japonicus (Cuvier et Va-lenciennes) (Lateolabracis) this health length 30%, feeding volume is the 10-12% of female Lateolabrax japonicus (Cuvier et Va-lenciennes) (Lateolabracis) TBW;Breeding the previous moon, bait is adding spirulina and the compound of root of Herba Houttuyniae composition of the 3-5% of the day amount of raising, was filled with ozone 1 hour every 2 days by ozonator, keeps water temperature to be 14-16 DEG C;
(2) parent fish selects: select body weight 0.8-1kg in pond, greenhouse, the good male parent fish of sexual maturity;Net cage is selected body weight 0.6-0.8kg, the good female parent fish of sexual maturity;
(3) artificial induced spawning: adopt odinagogue to hasten parturition to therein 1/5 respectively the male parent fish selected and female parent fish, the consumption that makes of described male parent fish odinagogue is luteinizing hormone releasing hormone analog 4-5 μ g/ tail, chorionic gona dotropin 2.2-2.4 μ g/ tail and thyroxine 1.6-1.8 μ g/ tail, the consumption that makes of female parent fish odinagogue is luteinizing hormone releasing hormone analog 4.8-6 μ g/ tail, chorionic gona dotropin 3.2-3.6 μ g/ tail and thyroxine 2.5-3.2 μ g/ tail, then in milter: the ratio that raun is 1:1.5-2 is put in same net cage, stimulate with the current of 0.2-0.25m/s, when the female parent fish abdominal part of light pressure has ovum to flow out, female parent fish and male parent fish are separated, carry out dry method of artificial fertilization;
(4) fry hatching: adopt circuit Lotic hatching, ensure that water quality is pure and fresh, current are unobstructed, control water temperature 15-18 DEG C to keep 8 hours, 13-15 DEG C keeps 16 hours, salinity is 2.2-2.4%, suitable increased water flow speed before and after membrane, removes in time egg membrane and foreign material in circuit, and after nursery is emerged three days and at present the pool is cultivated.
After part parent fish is hastened parturition, it is possible to induction parent fish gonad maturity, what not only reduce odinagogue makes consumption, it is also possible to reduce odinagogue amount in germ cell to a certain extent;
Concrete, pulverize when described leaf lettuce is dried to water content less than 7%, add in granulation machine and process and get final product.
Containing abundant nutritional labeling in leaf lettuce, including protein, fat, saccharide, ash, nicotinic acid, provitamin A, vitamin B1, vitamin B2, vitamin C, microelements of calcium, phosphorus, ferrum, magnesium, potassium, silicon etc., the metabolic function of cell can be promoted, promote the secretion of Fish androgen, contribute to the raising of milter genital gland indices.
Concrete, the mixed proportion of described compound spirulina and root of Herba Houttuyniae is 3:2;The preparation method of compound is: be first ground into powder by spirulina standby, then by Herba Houttuyniae with, after being equivalent to its soak by water of 3-4 times 40-60 minute, filtering, takes filtrate cooling concentrate drying powder process, is mixed with spirulina powder by gained powder, to obtain final product.
Spirulina and root of Herba Houttuyniae are mixed in proportion is strengthening the addition of cultivation later stage, not only facilitate female Lateolabrax japonicus (Cuvier et Va-lenciennes) (Lateolabracis) oogonium mitosis, parent fish ovulation amount is made to increase, it is favorably improved germ cell quantity, additionally aid the precipitation of oocyte nutrition, improve ovum quality, thus improving fertility rate and hatchability.
Concrete, after described ozonator is filled with ozone 1 hour, Measuring Ozone Concentration in Water is 0.4-0.6mg/L, regularly passes into a certain amount of ozone in the reinforcement cultivation later stage, it is possible to stimulate parent fish acceleration ripe, sexual maturity can be promoted to shift to an earlier date 15-20 days than commonsense method.
The present invention has the advantage that the strengthening parent fish cultivates the stage compared to existing technology, the female male parent of Lateolabrax japonicus (Cuvier et Va-lenciennes) (Lateolabracis) is cultivated respectively, physiological characteristics for female male parent carries out different management, make the period of sexual maturity of parent in advance, optimize the genital gland indices of milter, improve ovum quality and ovulation amount, and then improve fertility rate and hatchability, reduce labor intensity and the reproductive-cost of artificial propagation, contribute to the large-scale promotion of Lateolabrax japonicus (Cuvier et Va-lenciennes) (Lateolabracis) artificial propagation.
Detailed description of the invention
Embodiment 1
A kind of low cost propagation method of Lateolabrax japonicus (Cuvier et Va-lenciennes) (Lateolabracis), after the beginning of spring, being cultivated respectively by sexually matured male and female Lateolabrax japonicus (Cuvier et Va-lenciennes) (Lateolabracis), male Lateolabrax japonicus (Cuvier et Va-lenciennes) (Lateolabracis) presses 5-6 tail/cultivation in pond, greenhouse, start aerator, pond, the greenhouse depth of water is 0.5 meter, and salinity is 2.8-3.2%, and pH value is 7.2-8, every water of 4 Tianchong, maintain water temperature at and be stepped up between 8-18 DEG C, agreeable to the taste living bait fish of regularly throwing something and feeding, it is ensured that bait is sufficient;In breeding first half of the month, bath in every two days once, was filled with ozone 1 hour every 3 days by ozonator, keeps water temperature to be 16-20 DEG C, reduces the feeding volume 10% of living bait fish, adopted the made granule of leaf lettuce to replace;Raun adopts net cage to cultivate, the deep 3m of net cage, square is a dried up 20cm, salinity is 2.3-2.6%, and pH value is 6.5-7.2, breeding density be 6-8 tail/, maintain water temperature at and be stepped up between 10-20 DEG C, a bait of throwing something and feeding every day, described bait is live bait, including the forage fiss 70% and the bait shrimp 30% that are equivalent to Lateolabrax japonicus (Cuvier et Va-lenciennes) (Lateolabracis) this health length 30%, feeding volume is the 10-12% of female Lateolabrax japonicus (Cuvier et Va-lenciennes) (Lateolabracis) TBW;Breeding the previous moon, in bait, the spirulina of the 5% of the interpolation day amount of raising and the compound of root of Herba Houttuyniae composition, be filled with ozone 1 hour every 2 days by ozonator, keeps water temperature to be 14-16 DEG C;
Pond, greenhouse is selected body weight 1.5-2kg, the good male parent fish of sexual maturity;Net cage is selected body weight 2-3kg, the good female parent fish of sexual maturity;
Odinagogue is adopted to hasten parturition to therein 1/5 respectively the male parent fish selected and female parent fish, the consumption that makes of described male parent fish odinagogue is luteinizing hormone releasing hormone analog 4-5 μ g/ tail, chorionic gona dotropin 2.2-2.4 μ g/ tail and thyroxine 1.6-1.8 μ g/ tail, the consumption that makes of female parent fish odinagogue is luteinizing hormone releasing hormone analog 4.8-6 μ g/ tail, chorionic gona dotropin 3.2-3.6 μ g/ tail and thyroxine 2.5-3.2 μ g/ tail, then in milter: the ratio that raun is 1:1.5 is put in same net cage, stimulate with the current of 0.2-0.25m/s, when the female parent fish abdominal part of light pressure has ovum to flow out, female parent fish and male parent fish are separated, carry out dry method of artificial fertilization;
Adopt circuit Lotic hatching, it is ensured that water quality is pure and fresh, current are unobstructed, control water temperature 15-18 DEG C and keep 8 hours, 13-15 DEG C keeps 16 hours, and salinity is 2.2-2.4%, suitable increased water flow speed before and after membrane, removing in time egg membrane and foreign material in circuit, after nursery is emerged three days and at present the pool is cultivated.
Embodiment 2
A kind of low cost propagation method of Lateolabrax japonicus (Cuvier et Va-lenciennes) (Lateolabracis), substantially the same manner as Example 1, it is different in that:
(1) the previous moon is being bred, the compound that the spirulina of the 3% of the interpolation day amount of raising forms in bait with root of Herba Houttuyniae;
(2) after hastening parturition, milter: raun is 1:2.
When other management methods are identical, the adding proportion properly increasing compound can make female Gunther parent fish spawning rate be greatly increased, and therefore can reduce the ratio of raun accordingly.
Set up matched group, in strengthening cultivation stage male and female parent fish mixed breed according to a conventional method management, after hastening parturition, milter: raun is 1:1, after the beginning of spring, unified beginning strengthens cultivation, carries out nursery after incubating oosperm respectively under identical condition, add up and record corresponding data, being concluded that
Group | Hasten parturition the phase | Spawning rate (%) | Rate of fertilization (%) | Incubation rate (%) | Survival rate of fish fry (%) |
Matched group | The last ten-days period in March | 81.3 | 76.5 | 74.3 | 76.4 |
Embodiment 1 | The first ten-day period of the March | 85.6 | 83.3 | 88.5 | 85.6 |
Embodiment 2 | The first ten-day period of the March | 86.8 | 82.6 | 86.6 | 87.8 |
Table 1
Decrease female parent fish in an embodiment accordingly, reduce certain reproductive-cost, reduce the use of catalyst simultaneously, it is ensured that the health of fry.
Claims (4)
1. the low cost propagation method of a Lateolabrax japonicus (Cuvier et Va-lenciennes) (Lateolabracis), it is characterised in that comprise the following steps:
(1) parent fish is cultivated: after the beginning of spring, sexually matured male and female Lateolabrax japonicus (Cuvier et Va-lenciennes) (Lateolabracis) is cultivated respectively, male Lateolabrax japonicus (Cuvier et Va-lenciennes) (Lateolabracis) presses 5-6 tail/cultivation in pond, greenhouse, starts aerator, and pond, the greenhouse depth of water is 0.5 meter, salinity is 2.8-3.2%, pH value is 7.2-8, every water of 4 Tianchong, maintains water temperature at and is stepped up between 8-18 DEG C, regularly throw something and feed agreeable to the taste living bait fish, it is ensured that bait is sufficient;In breeding first half of the month, bath in every two days once, was filled with ozone 1 hour every 3 days by ozonator, keeps water temperature to be 16-20 DEG C, reduces the feeding volume 10% of living bait fish, adopted the made granule of leaf lettuce to replace;
Raun adopts net cage to cultivate, the deep 3m of net cage, square is a dried up 20cm, salinity is 2.3-2.6%, and pH value is 6.5-7.2, breeding density be 6-8 tail/, maintain water temperature at and be stepped up between 10-20 DEG C, a bait of throwing something and feeding every day, described bait is live bait, including the forage fiss 70% and the bait shrimp 30% that are equivalent to Lateolabrax japonicus (Cuvier et Va-lenciennes) (Lateolabracis) this health length 30%, feeding volume is the 10-12% of female Lateolabrax japonicus (Cuvier et Va-lenciennes) (Lateolabracis) TBW;Breeding the previous moon, bait is adding spirulina and the compound of root of Herba Houttuyniae composition of the 3-5% of the day amount of raising, was filled with ozone 1 hour every 2 days by ozonator, keeps water temperature to be 14-16 DEG C;
(2) parent fish selects: select body weight 0.8-1kg in pond, greenhouse, the good male parent fish of sexual maturity;Net cage is selected body weight 0.6-0.8kg, the good female parent fish of sexual maturity;
(3) artificial induced spawning: adopt odinagogue to hasten parturition to therein 1/5 respectively the male parent fish selected and female parent fish, the consumption that makes of described male parent fish odinagogue is luteinizing hormone releasing hormone analog 4-5 μ g/ tail, chorionic gona dotropin 2.2-2.4 μ g/ tail and thyroxine 1.6-1.8 μ g/ tail, the consumption that makes of female parent fish odinagogue is luteinizing hormone releasing hormone analog 4.8-6 μ g/ tail, chorionic gona dotropin 3.2-3.6 μ g/ tail and thyroxine 2.5-3.2 μ g/ tail, then in milter: the ratio that raun is 1:1.5-2 is put in same net cage, stimulate with the current of 0.2-0.25m/s, when the female parent fish abdominal part of light pressure has ovum to flow out, female parent fish and male parent fish are separated, carry out dry method of artificial fertilization;
(4) fry hatching: adopt circuit Lotic hatching, ensure that water quality is pure and fresh, current are unobstructed, control water temperature 15-18 DEG C to keep 8 hours, 13-15 DEG C keeps 16 hours, salinity is 2.2-2.4%, suitable increased water flow speed before and after membrane, removes in time egg membrane and foreign material in circuit, and after nursery is emerged three days and at present the pool is cultivated.
2. the low cost propagation method of a kind of Lateolabrax japonicus (Cuvier et Va-lenciennes) (Lateolabracis) as claimed in claim 1, it is characterised in that pulverize when described leaf lettuce is dried to water content less than 7%, adds in granulation machine and processes and get final product.
3. the low cost propagation method of a kind of Lateolabrax japonicus (Cuvier et Va-lenciennes) (Lateolabracis) as claimed in claim 1, it is characterised in that the mixed proportion of described compound spirulina and root of Herba Houttuyniae is 3:2;The preparation method of compound is: be first ground into powder by spirulina standby, then by Herba Houttuyniae with, after being equivalent to its soak by water of 3-4 times 40-60 minute, filtering, takes filtrate cooling concentrate drying powder process, is mixed with spirulina powder by gained powder, to obtain final product.
4. the low cost propagation method of a kind of Lateolabrax japonicus (Cuvier et Va-lenciennes) (Lateolabracis) as claimed in claim 1, it is characterised in that after described ozonator is filled with ozone 1 hour, Measuring Ozone Concentration in Water is 0.4-0.6mg/L.
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