CN110063276A - A kind of method of colored perch artificial propagation and its use feed - Google Patents
A kind of method of colored perch artificial propagation and its use feed Download PDFInfo
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- CN110063276A CN110063276A CN201910281757.5A CN201910281757A CN110063276A CN 110063276 A CN110063276 A CN 110063276A CN 201910281757 A CN201910281757 A CN 201910281757A CN 110063276 A CN110063276 A CN 110063276A
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- 239000003814 drug Substances 0.000 claims abstract description 21
- 230000005070 ripening Effects 0.000 claims abstract description 20
- 239000004615 ingredient Substances 0.000 claims abstract description 16
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims abstract description 14
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 14
- 239000000654 additive Substances 0.000 claims abstract description 14
- 230000000996 additive effect Effects 0.000 claims abstract description 14
- 230000012447 hatching Effects 0.000 claims abstract description 14
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- 241000238366 Cephalopoda Species 0.000 claims abstract description 8
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- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 8
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- 241000237502 Ostreidae Species 0.000 claims abstract description 8
- 229930003779 Vitamin B12 Natural products 0.000 claims abstract description 8
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 claims abstract description 8
- 210000004681 ovum Anatomy 0.000 claims abstract description 8
- 235000020636 oyster Nutrition 0.000 claims abstract description 8
- 235000019163 vitamin B12 Nutrition 0.000 claims abstract description 8
- 239000011715 vitamin B12 Substances 0.000 claims abstract description 8
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims abstract description 7
- 229930003268 Vitamin C Natural products 0.000 claims abstract description 7
- 229930003427 Vitamin E Natural products 0.000 claims abstract description 7
- 230000004720 fertilization Effects 0.000 claims abstract description 7
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims abstract description 7
- 235000019154 vitamin C Nutrition 0.000 claims abstract description 7
- 239000011718 vitamin C Substances 0.000 claims abstract description 7
- 235000019165 vitamin E Nutrition 0.000 claims abstract description 7
- 239000011709 vitamin E Substances 0.000 claims abstract description 7
- 229940046009 vitamin E Drugs 0.000 claims abstract description 7
- 238000012216 screening Methods 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 32
- 235000013601 eggs Nutrition 0.000 claims description 16
- 235000021401 pellet diet Nutrition 0.000 claims description 12
- 230000032696 parturition Effects 0.000 claims description 9
- 230000002196 ecbolic effect Effects 0.000 claims description 8
- RRAFCDWBNXTKKO-UHFFFAOYSA-N eugenol Chemical compound COC1=CC(CC=C)=CC=C1O RRAFCDWBNXTKKO-UHFFFAOYSA-N 0.000 claims description 8
- 239000002994 raw material Substances 0.000 claims description 5
- NPBVQXIMTZKSBA-UHFFFAOYSA-N Chavibetol Natural products COC1=CC=C(CC=C)C=C1O NPBVQXIMTZKSBA-UHFFFAOYSA-N 0.000 claims description 4
- 239000005770 Eugenol Substances 0.000 claims description 4
- UVMRYBDEERADNV-UHFFFAOYSA-N Pseudoeugenol Natural products COC1=CC(C(C)=C)=CC=C1O UVMRYBDEERADNV-UHFFFAOYSA-N 0.000 claims description 4
- 229960002217 eugenol Drugs 0.000 claims description 4
- 210000003205 muscle Anatomy 0.000 claims description 4
- 108700012941 GNRH1 Proteins 0.000 claims description 3
- 239000000579 Gonadotropin-Releasing Hormone Substances 0.000 claims description 3
- 230000037396 body weight Effects 0.000 claims description 3
- 230000008859 change Effects 0.000 claims description 3
- 238000002347 injection Methods 0.000 claims description 2
- 239000007924 injection Substances 0.000 claims description 2
- 238000007913 intrathecal administration Methods 0.000 claims description 2
- 230000001737 promoting effect Effects 0.000 claims 1
- 239000003488 releasing hormone Substances 0.000 claims 1
- 235000019688 fish Nutrition 0.000 abstract description 26
- 241001430403 Coilia grayii Species 0.000 abstract description 6
- 241001502129 Mullus Species 0.000 abstract description 6
- 241000736084 Scomber japonicus Species 0.000 abstract description 6
- 230000026109 gonad development Effects 0.000 abstract description 5
- 238000009395 breeding Methods 0.000 abstract description 4
- 230000001488 breeding effect Effects 0.000 abstract description 4
- 230000017448 oviposition Effects 0.000 abstract description 3
- 238000004064 recycling Methods 0.000 abstract 1
- 230000000052 comparative effect Effects 0.000 description 5
- 239000005556 hormone Substances 0.000 description 4
- 229940088597 hormone Drugs 0.000 description 4
- 230000000384 rearing effect Effects 0.000 description 4
- 210000002969 egg yolk Anatomy 0.000 description 3
- 238000009364 mariculture Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 235000012054 meals Nutrition 0.000 description 3
- 241000276618 Perciformes Species 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000004584 weight gain Effects 0.000 description 2
- 235000019786 weight gain Nutrition 0.000 description 2
- 241000922680 Cromileptes altivelis Species 0.000 description 1
- 241000321429 Epinephelus itajara Species 0.000 description 1
- 241000269794 Lateolabrax Species 0.000 description 1
- 241001287943 Lateolabrax maculatus Species 0.000 description 1
- 241001417495 Serranidae Species 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 230000002710 gonadal effect Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
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- 235000016709 nutrition Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 230000016087 ovulation Effects 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Marine Sciences & Fisheries (AREA)
- Environmental Sciences (AREA)
- Birds (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Insects & Arthropods (AREA)
- Farming Of Fish And Shellfish (AREA)
- Fodder In General (AREA)
Abstract
The purpose of the present invention is to provide a kind of method of colored perch artificial propagation and its feed is used, the use of feed includes major ingredient and additive, major ingredient includes the trash fishes such as perch mixed feed, mullet, long-tailed anchovy, chub mackerel, fresh shrimp, squid and oyster；Additive includes that vitamin B12, vitamin C, vitamin E and gonad maturity accelerating are precious；The propagation method is the following steps are included: 1) parent population screening and fortification；2) it accelerates the ripening and artificial induced spawning；3) fertilization ovum collecting and hatching.Parent population constitution can be remarkably reinforced in the present invention, promote gonad development, optimize smart archiblast amount, improve gamete quality, while improving parent population recycling rate of waterused, using appropriate drug dose accelerate the ripening and artificial induced spawning, so that parent population is concentrated oviposition, reduces the labor intensity and reproductive-cost of artificial propagation；The flower perch artificial fecundation method success rate of use is high, can effectively improve colored perch seed yield, meet scale breeding demand.
Description
Technical field
The present invention relates to technical field of aquaculture, and in particular to a kind of method and its use feeding of colored perch artificial propagation
Material.
Background technique
Flower perch (Lateolabrax maculatus) is also known as jewfish, seven-star perch, belongs to Perciformes (Perciformes) , Sushi section
(Serranidae), flower perch category (Lateolabrax).It is its delicious meat, full of nutrition, it is deep to be liked by the majority of consumers；Simultaneously
The flower perch speed of growth is fast, wider to the adaptation range of salinity and temperature, becomes one of important breed variety of Chinse Coastal Area,
2017, whole nation flower perch cultured output was up to 15.66 ten thousand tons.
Based on huge breeding prospect, flower perch scale and the supply of stable seed have become urgent need, spend perch at present
Artificial breeding technique is very mature, but that there are still parent populations is of poor quality for traditional method for culturing seedlings, operates easily dead, and seed constitution is weak
Etc. many defects.
There is Patent document data to disclose the relevant technologies of aquaculture in the prior art, such as CN201711027357.9 mono-
The artificial fecundation method of kind analysis of Cromileptes altivelis and a kind of perch artificial propagation of patent CN201810764021.9 and seed rearing side
Method discloses its corresponding artificial fecundation method, but these technical methods are not suitable for spending the breeding of perch, after study, this
Invention provides a kind of artificial fecundation method suitable for flower perch.
Summary of the invention
Against the above technical problems, the purpose of the present invention is to provide a kind of methods of colored perch artificial propagation and its use to raise
Material solves traditional propagation method and there is the defects of cultivation fish of poor quality, operation dead, fingerling constitution is weak.
Technical scheme is as follows:
A kind of use feed of colored perch artificial propagation, is soft pellet diet, including major ingredient and additive, major ingredient includes that perch is matched
Close trash fishes, fresh shrimp, squid and the oysters such as feed, mullet, long-tailed anchovy, chub mackerel；Additive includes vitamin B12, vitamin C, vitamin E
And gonad maturity accelerating is precious；
In major ingredient, as mass fraction, perch mixed feed accounts for 30% ~ 40%, and the trash fishes such as mullet, long-tailed anchovy, chub mackerel account for 20% ~ 30%, fresh shrimp
10% ~ 15% is accounted for, squid accounts for 10% ~ 15% and oyster 5% ~ 10%；Additive component is based on the mass fraction for accounting for major ingredient, vitamin
B12, which accounts for 0.1% ~ 0.2%, vitamin C and accounts for 0.2% ~ 0.3%, vitamin E and account for 0.3% ~ 0.5%, gonad maturity accelerating treasured, accounts for 0.5% ~ 1%, wherein
Perch mixed feed is that the 9# purchased from Fujian heavenly steed group is good for horse board perch mixed feed, and gonad maturity accelerating treasured is dynamic purchased from Dalian Austria peptide
Object nutrition Co., Ltd；
Proportionally all raw materials are rubbed and are mixed, are pelletized by soft pellet diet blender, partial size is 10 ~ 13mm, is raised
3~5 DEG C of preservations after material is made.
A kind of method of colored perch artificial propagation, comprising the following steps:
1) parent population screening and fortification: 22 ~ 24 DEG C of water temperature, select that physically fit, disease-free and injury-free, gonad development is good 9 ~ October
Good cage culture individual is reserve parent fish, it is desirable that more than age male 3, weight 3.5kg or more；Female 4 is more than age, weight 5kg
More than；Reserve parent fish is individually cultivated in marine net cage, mariculture area requires water quality good, and daily two meal feeds the soft particle of parent population
Feed carries out fortification, and day feeding volume is the 8 ~ 10% of fish body weight, and the fortification period is no less than 8 weeks, specific to regard parent population property
Depending on gland developmental state；
2) accelerate the ripening and artificial induced spawning: in 11 ~ December, water temperature can carry out parent population lower than 20 DEG C and accelerate the ripening and artificial induced spawning, in net cage
Canvas water carrier, water inlet plus eugenol are hung, parent population is fished out in batches into wherein anaesthetizing；Select the good individual of gonadal maturation degree
Muscle of back or intrathecal is carried out to accelerate the ripening drug and oxytocic drug；
3) fertilization ovum collecting and hatching: parent population, which is hastened parturition, to lay eggs after 60h, collects fertilized eggs in hatching barrel with 80 ~ 100 mesh trawlnets,
Micro- inflation is hatched；
Canvas water carrier can be used if pond culture, the fertilized eggs for collecting floating are put into water bag, and micro- inflation is hatched, per acre may be used
1 ~ 2kg fertilized eggs are put, prelarva yolk bag absorbing state is observed after hatching, water bag is unlocked before it is fully absorbed and releases fry, with
Enter seed rearing link；
Net cage puts on 80 ~ 100 mesh yarn tulles in step 1), is placed at 6 ~ 10m of the depth of water, 0.4 ~ 0.8m/s of water velocity；
The drug that accelerates the ripening of step 2 includes Luteinizing hormone releasing-hormone A3 (LHRH-A3), is accelerated the ripening drug 7 ~ 10 days to parent population injection
Inject oxytocic drug again afterwards, oxytocic drug includes Luteinizing hormone releasing-hormone A3 (LHRH-A3) and human chorionic gonadtropin
(HCG) be used in mixed way；
The drug dose that accelerates the ripening in step 2 is that every kilogram of fish injects 1 ~ 2 μ g LHRH-A3, and oxytocic drug dosage is every kilogram of fish note
10 ~ 20 μ g LHRH-A3 and 100 ~ 200IU HCG are penetrated, male dosage halves；
The female-male proportion of artificial induced spawning parent population is 2 ~ 3:1 in step 2.
Stop the supple of gas or steam after 2 ~ 3h of micro- inflation 20 ~ 30min in step 3), inhales bottom and removes dead ovum and impurity, and it is primary to change water by every 6h later,
Quantity of exchanged water is 1/3；20 DEG C of water temperature, fertilized eggs 50h or so incubation of membrane.
Beneficial effects of the present invention are as follows:
The method of flower perch artificial propagation of the invention and feed is used, the feed of the enriched nutritive used compares traditional skill
Parent population constitution can be remarkably reinforced in art, promote gonad development, optimize smart archiblast amount, improve gamete quality, while improving parent population repetition
Utilization rate, using appropriate drug dose accelerate the ripening and artificial induced spawning, so that parent population is concentrated oviposition, reduce artificial propagation
Labor intensity and reproductive-cost；
The flower perch artificial fecundation method success rate that the present invention uses is high, can effectively improve colored perch seed yield, meet scale and educate
Seedling demand.
Specific embodiment
Below by way of specific case study on implementation, the present invention is described in further detail, it should be understood that these embodiments are only used
In illustrating the present invention rather than limit the scope of the invention, after the present invention has been read, those skilled in the art couple
It is as defined in the appended claims that the modification of various equivalent forms of the invention falls within the application.
Embodiment 1
A kind of use feed of colored perch artificial propagation:
It is soft pellet diet, including major ingredient and additive, in major ingredient, takes perch mixed feed 40%, the trash fishes such as mullet, long-tailed anchovy, chub mackerel
25%, fresh shrimp 10%, squid 15% and oyster 10%；
In additive, vitamin B12 0.1%, vitamin C 0.3%, vitamin E 0.4%, gonad maturity accelerating treasured 1% are taken,；
Proportionally all raw materials are rubbed and are mixed, are pelletized by soft pellet diet blender, partial size 13mm, feed system
It is saved at latter 4 DEG C.
Embodiment 2
A kind of use feed of colored perch artificial propagation:
It is soft pellet diet, including major ingredient and additive, in major ingredient, takes perch mixed feed 30%, the trash fishes such as mullet, long-tailed anchovy, chub mackerel
20%, fresh shrimp 15%, squid 10% and oyster 10%；
In additive, vitamin B12 0.2%, vitamin C 0.2%, vitamin E 0.3%, gonad maturity accelerating treasured 0.5% are taken,；
Proportionally all raw materials are rubbed and are mixed, are pelletized by soft pellet diet blender, partial size 10mm, feed system
It is saved at latter 5 DEG C.
Embodiment 3
A kind of use feed of colored perch artificial propagation:
It is soft pellet diet, including major ingredient and additive, in major ingredient, takes perch mixed feed 35%, the trash fishes such as mullet, long-tailed anchovy, chub mackerel
30%, fresh shrimp 10%, squid 10% and oyster 5%；
In additive, vitamin B12 0.1%, vitamin C 0.2%, vitamin E 0.5%, gonad maturity accelerating treasured 1% are taken,；
Proportionally all raw materials are rubbed and are mixed, are pelletized by soft pellet diet blender, partial size 12mm, feed system
It is saved at latter 3 DEG C.
Embodiment 4
A kind of method of colored perch artificial propagation:
(1) parent population screening and fortification
At the beginning of in Septembers, 2018, selecting 10 tail, 4 age male and 30 tail, 5 age female cage culture flower perch is reserve parent fish；Selected male flower
Perch average weight 4.6kg, female average weight 5.9kg.By reserve parent fish, at sea net cage is individually cultivated, and mariculture area water quality is good
It is good, 6.5 ~ 9m of the depth of water, 0.4 ~ 0.6m/s of water velocity.The soft pellet diet that daily two meal feeds the preparation of parent population embodiment 1 carries out
Fortification, day feeding volume are the 8 ~ 10% of fish body total weight；
(2) it accelerates the ripening and artificial induced spawning
Parent population fortification 10 weeks, until by the end of November, water temperature is down to 19 ~ 20 DEG C, start to carry out parent population accelerate the ripening and artificial induced spawning.?
Hang canvas water carrier in net cage, water inlet plus eugenol fish out parent population into wherein anaesthetizing.Muscle of back injects luteotropin release
Hormone A3(LHRH-A3) it accelerates the ripening, dosage is that every kilogram of fish injects 2 μ g；It hastens parturition after 7d, drug is discharged using luteotropin
Hormone A3(LHRH-A3) and human chorionic gonadtropin (HCG) be used in mixed way, dosage is that every kilogram of fish injects 20 μ g LHRH-A3
+ 200IU HCG, milter dosage halve；
(3) fertilization ovum collecting and hatching
80 mesh yarn tulles are covered in former net cage, the parent population after hastening parturition is put into wherein, start to lay eggs successively after parent population 64h, with 80
Mesh trawlnet collects fertilized eggs in hatching barrel, and filtering cleaning is transferred to pond hatching water bag after removing sundries, and micro- inflation is hatched, often
Mu puts 1.5kg fertilized eggs, and prelarva yolk bag absorbing state is observed after hatching, and water bag is unlocked before it is fully absorbed and releases fry,
Enter seed rearing link immediately.
Embodiment 5
A kind of method of colored perch artificial propagation:
(1) parent population screening and fortification
At the beginning of in Septembers, 2018, selecting 10 tail, 4 age male and 20 tail, 5 age female cage culture flower perch is reserve parent fish；Selected male flower
Perch average weight 4.8kg, female average weight 5.6kg.By reserve parent fish, at sea net cage is individually cultivated, and mariculture area water quality is good
It is good, 6 ~ 10m of the depth of water, 0.5 ~ 0.8m/s of water velocity.The soft pellet diet that daily two meal feeds the preparation of parent population embodiment 1 is sought
It supports and strengthens, day feeding volume is the 8 ~ 10% of fish body total weight；
(2) it accelerates the ripening and artificial induced spawning
Parent population fortification 10 weeks, until by the end of November, water temperature is down to 19 ~ 20 DEG C, start to carry out parent population accelerate the ripening and artificial induced spawning.?
Hang canvas water carrier in net cage, water inlet plus eugenol fish out parent population into wherein anaesthetizing.Muscle of back injects luteotropin release
Hormone A3(LHRH-A3) it accelerates the ripening, dosage is that every kilogram of fish injects 1 μ g；It hastens parturition after 10d, drug is released using luteotropin
Put hormone A3(LHRH-A3) and human chorionic gonadtropin (HCG) be used in mixed way, dosage is that every kilogram of fish injects 10 μ g LHRH-
A3+200IU HCG, milter dosage halve；
(3) fertilization ovum collecting and hatching
100 mesh yarn tulles are covered in former net cage, the parent population after hastening parturition is put into wherein, start to lay eggs successively after parent population 58h, are used
100 mesh trawlnets collect fertilized eggs in hatching barrel, and filtering cleaning is transferred to pond hatching water bag after removing sundries, and micro- inflation is incubated
Change, put 1.4kg fertilized eggs per acre, prelarva yolk bag absorbing state is observed after hatching, water bag releasing is unlocked before it is fully absorbed
Fry enters seed rearing link immediately.
The present invention also sets up comparative confirmatory experiment:
Comparative example 1
Comparative example 1 and embodiment 4 are the parent population selected with a batch, male 10 tails, average weight 4.5kg, female 20 tails, average body
Weight 5.8kg；
The two difference is in 1 step of comparative example (1) that parent population feeds trash fish, day feeding volume is not through fortification sooner or later daily
The 8 ~ 10% of fish body total weight；
As a result:
4 parent population of embodiment weight gain after fortification in 10 weeks is obvious, and gonad development is good, after hastening parturition parent population ovulation period compared with
To concentrate, 90% or more, prelarva is physical strong for ovum fertilization rate and hatchability of fertile eggs, and travelling ability is stronger.Comparative example 1
Parent population weight gain is obvious, but gonad development is general, and 96h has not yet to see oviposition after hastening parturition for the first time, carries out same drug agent immediately
Flow control is secondary to hasten parturition, and lays eggs successively after 72h, the fertilized eggs negligible amounts being collected into, ovum fertilization rate 60 ~ 70%, incubating oosperm
Rate 80% or so, fry quality is poor, and survival rate is low.
Claims (10)
1. a kind of colored perch artificial propagation uses feed, which is characterized in that it is soft pellet diet, including major ingredient and additive,
Major ingredient includes perch mixed feed, trash fish, fresh shrimp, squid and oyster；Additive includes vitamin B12, vitamin C, dimension
Raw element E and gonad maturity accelerating are precious.
2. using feed by colored perch artificial propagation described in claim 1, which is characterized in that in the major ingredient of soft pellet diet,
As mass fraction, perch mixed feed accounts for 30% ~ 40%, and trash fish accounts for 20% ~ 30%, and fresh shrimp accounts for 10% ~ 15%, and squid accounts for 10% ~
15% and oyster account for 5% ~ 10%；For additive component based on the mass fraction for accounting for major ingredient, vitamin B12 accounts for 0.1% ~ 0.2%, dimension life
Plain C, which accounts for 0.2% ~ 0.3%, vitamin E and accounts for 0.3% ~ 0.5%, gonad maturity accelerating treasured, accounts for 0.5% ~ 1%.
3. using feed by colored perch artificial propagation of any of claims 1 or 2, which is characterized in that all raw materials, which pass through, to be rubbed,
The partial size of obtained feed is 10 ~ 13mm.
4. a kind of method of colored perch artificial propagation, which comprises the following steps:
1) parent population screening and fortification: reserve parent fish is selected, marine net cage is placed in and individually cultivates, feed parent population claim 1
Described in any item using feed progress fortification to 3, day feeding volume position is the 8% ~ 10% of fish body weight；
2) it accelerates the ripening and artificial induced spawning: anaesthetizing parent population with eugenol, select that parent population carries out muscle of back or intrathecal accelerates the ripening drug
And oxytocic drug；
3) fertilization ovum collecting and hatching: parent population lays eggs after being hastened parturition, micro- to fill with 80 ~ 100 mesh trawlnets collection fertilized eggs in hatching barrel
Gas is hatched.
5. by the method for colored perch artificial propagation as claimed in claim 4, which is characterized in that reserve parent fish requires male in step 1)
3 more than age, weight 3.5kg or more；Female 4 is more than age, weight 5kg or more；Net cage puts on 80 ~ 100 mesh yarn tulles, is placed in the depth of water
At 6 ~ 10m, 0.4 ~ 0.8m/s of water velocity.
6. by the method for colored perch artificial propagation as claimed in claim 4, which is characterized in that the drug that accelerates the ripening of step 2 includes promoting Huang
Voxel releasing hormone A3 LHRH-A3 injects oxytocic drug after accelerating the ripening drug 7 ~ 10 days to parent population injection again.
7. the method for flower perch artificial propagation as described in claim 4 or 6, which is characterized in that oxytocic drug includes in step 2
Luteinizing hormone releasing-hormone A3 LHRH-A3's and human chorionic gonadtropin HCG is used in mixed way.
8. by the method for colored perch artificial propagation as claimed in claim 7, which is characterized in that the drug dose that accelerates the ripening in step 2 is every
Kilogram fish injects 1 ~ 2 μ g LHRH-A3, oxytocic drug dosage be every kilogram of fish inject 10 ~ 20 μ g LHRH-A3 and 100 ~
200IU HCG。
9. the method for flower perch artificial propagation as described in claim 4 or 6, which is characterized in that artificial induced spawning parent population in step 2
Female-male proportion be 2 ~ 3:1.
10. by the method for colored perch artificial propagation as claimed in claim 4, which is characterized in that stop after 2 ~ 3h of micro- inflation in step 3)
20 ~ 30min of gas, it is primary to change water by every 6h later.
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