CN110100769A - A kind of artificial fecundation method of red lapo-lapo - Google Patents
A kind of artificial fecundation method of red lapo-lapo Download PDFInfo
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- CN110100769A CN110100769A CN201910467241.XA CN201910467241A CN110100769A CN 110100769 A CN110100769 A CN 110100769A CN 201910467241 A CN201910467241 A CN 201910467241A CN 110100769 A CN110100769 A CN 110100769A
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- lapo
- pond
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- parent population
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/22—Compounds of alkali metals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Food Science & Technology (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Physiology (AREA)
- Microbiology (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Inorganic Chemistry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Biomedical Technology (AREA)
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- Insects & Arthropods (AREA)
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- Botany (AREA)
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- Farming Of Fish And Shellfish (AREA)
Abstract
The present invention provides a kind of artificial fecundation method of red lapo-lapo, comprising steps of (1) Juvenile stage, (2) cultivate pond treatment, (3) parent fish rearing management, and a. is cultivated the 1st week, alternately irradiates the pond water surface using green light and yellow light；In cultivation the 2nd week~3 weeks, the pond water surface is alternately irradiated using green light and feux rouges；At cultivation 3 weeks or more, the pond water surface is irradiated using green light；B. feed is divided into grouper compound feed, fresh 1~2 part of small fish and shrimp and 5~8 parts of chilled trash fish, artificial soft pellet diet；It cultivates and feeds within first week the first feed, cultivation feeds second of feed for the 2nd week~3 weeks, feeds the third feed within cultivation 3 weeks or more；C. pond water temperature control is made as 23~27 DEG C, water dissolved oxygen > 5mg/L；(4) reinforced cultivating before breeding, (5) artificial propagation, (6) collecting fertilized eggs and hatching；The artificial fecundation method of red lapo-lapo of the invention, not only promotes the grazing rate of parent population, and enhances the fertility of parent population, improves the spawning rate and rate of fertilization of parent population, effectively realizes the red lapo-lapo of scale artificial culture.
Description
Technical field
The present invention relates to technical field of aquaculture, in particular to a kind of artificial fecundation method of red lapo-lapo.
Background technique
Red lapo-lapo, (Cephalopholis sonnerati) are commonly called as reticulate pattern Grouper, cross fish, lithosporic, red rudder, red melon seed
Spot, a kind of fish belonged to for spoke fin net-rope Perciformes perch Ya Mu Sushi section lapo-lapo.Body is red, and there are crineous in head and back front end
Reticulate pattern does not have spot and striped.Without canine tooth in the middle part of lower jaw, anal fin has 3 fin spine, 9 fin ray.Tail fin rear is round, does not have striped.Through
It often inhabits in the shallow sea of rock substrate, is food with invertebrate and fish, be tropical forest insect reef and littoral fish.Red nine spine
Edible fishes of the perch as a kind of top grade on existing market, that is consumed deeply likes, but at present for the artificial of red lapo-lapo
The research of propagation method is less, has not been able to carry out the artificial breeding that effective red lapo-lapo carries out plant layout, and survival rate is low,
Especially in reproductive process, the grazing rate of parent population is unstable, and the growth and development of parent population and fertility are low, spawning rate, by
Smart rate and hatching rate are lower, still cannot achieve the artificial propagation of effective red lapo-lapo, meet in the market to the need of red lapo-lapo
It asks.
Summary of the invention
In consideration of it, the present invention proposes a kind of artificial fecundation method of red lapo-lapo, taking the photograph for parent population can not only be effectively facilitated
Food rate, and the fertility of parent population is greatly strengthened, the spawning rate and rate of fertilization of parent population are improved, scale is effectively performed in realization
Change and propagates red lapo-lapo artificially.
The technical scheme of the present invention is realized as follows:
The present invention provides a kind of artificial fecundation method of red lapo-lapo, includes the following steps:
(1) Juvenile stage
Female parent: normal, disease-free, the energetic red lapo-lapo of selection 6 more than age, 3.5~5.5kg of weight, figure
As female parent；
Male parent: normal, disease-free, the energetic red lapo-lapo of selection 6 more than age, 3.2~5.5kg of weight, figure
As male parent；
(2) pond is cultivated
It selecting to cultivate the area in the pond of parent to be 2~3 mu, the depth of water is 4~6m, and bottom of pond is equipped with inlet and outlet,
LED light light device is provided with above pond；Cultivation density is 100~120 tails/mu, and female-male proportion is 1:(0.5~1)；
(3) parent fish rearing management
A. light control: in cultivation the 1st week, using LED green light and the yellow light alternately irradiation pond water surface, daily every kind photochromic
Continuous irradiation 6~8 hours；In cultivation the 2nd week~3 weeks, the pond water surface is alternately irradiated using LED green light and feux rouges, it is every daily
Kind photochromic Continuous irradiation 8~12 hours；At cultivation 3 weeks or more, the pond water surface, daily Continuous irradiation 12 are irradiated using LED green light
~16 hours；Wherein, the wavelength of green light is 445~465nm, the wavelength of yellow light is 460~480nm, the wavelength 570 of feux rouges~
700nm；
B. feed and feed: feed is divided into three kinds；The first is grouper compound feed, and second is fresh 1~2 part small
Fishes and shrimps and 5~8 parts of chilled trash fish, it with mass ratio is 6 that the third, which is by grouper compound feed, chilled trash fish and spirulina powder:
(3~1): (2~1) mixing, the artificial soft pellet diet that system material is processed into；It is thrown in cultivation first week using the first feed
Hello, second of feedstuff feeding is used when cultivating the 2nd week~3 weeks, the third feedstuff feeding was used at cultivation 3 weeks or more, daily
It feeds feed 1 time, each feeding volume is the 2.8%-3.5% of parent population weight；
C. water temperature control and water quality management: pond water temperature control is made as 23~27 DEG C, and water dissolved oxygen > 5mg/L changes water in every 3 days
1 time, quantity of exchanged water is the 20~23% of pond water deep water amount, keeps water transparency in 35~45cm；
(4) reinforced cultivating before breeding
A. cultivate water temperature: the reinforced cultivating of parent population is carried out from November between March, from November adjust pond water temperature be 20~
23 DEG C, until adjusting pond water temperature is 23~25 DEG C, until pond water temperature is risen to 25~27 DEG C when March when January；
B. feed and dosage in reinforced cultivating: feed is the Three feed in step (3), and Three feed rotation is used,
And every kind of feed is continuously fed 6~8 days, is fed within every 2 days once, each feeding volume is the 2.0~2.5% of parent population weight；
C. water quality management: changing water 1 time for every 5 days, and quantity of exchanged water is the 22%~28% of pond water, and water transparency is kept to exist
50cm or more:
(5) artificial propagation
Start March to progressively reach sexual gland maturation by the parent population of reinforced cultivating, be observed in daily cultivating and managing, once hair
Existing parent population body colour deepens, and can carry out artificial induced spawning: before hastening parturition, by lower water temperature to 26~27 DEG C, by parent population from culture pond
The pool has been caught, and selects that abdomen is big, the micro- red individual of gonopore, injects ocyodinic, injection time to parent population using intraperitoneal injection mode
To be injected when 9:00-11:00 in morning, and parent population is put back into pond immediately, after carrying out 2~3 days of artificial induced spawning, parent population
Start gradually to lay eggs；
(6) collecting fertilized eggs and hatching
A. when collecting fertilized eggs, fertilized eggs is collected using collection ovum net, is rinsed with fresh seawater, removes bad ovum and sundries；
B. fertilized eggs are put into a volume 500L, bottom equipped with hatching in the hatching barrel of drain valve, are put in hatching barrel
The inflation of gas stone is set, incubating oosperm condition is 24-26 DEG C of water temperature, salinity 29.3 ± 0.7, pH8.21 ± 0.3, and hatching density is
Ten thousand fertilized eggs/m of 230-2503, change within every 3 hours that water is primary, each quantity of exchanged water is the half of hatching barrel volume, to keep hatching
Seawater is pure and fresh in bucket.
The present invention proposes a kind of artificial fecundation method of red lapo-lapo, mainly passes through the process in parent fish rearing management
In, it uses LED green light, yellow light and feux rouges and carries out interim alternately irradiation, to substantially increase the food ration of parent population, meanwhile,
It further uses the corresponding stage of Three feed progress to feed, and corresponding water temperature control, and then is not only effectively ensured
The food ration of parent population, and be conducive to the absorption of the nutritional ingredient of parent population, improve the growth and development quality of parent population；And in parent population
Breeding early period reinforced cultivating, also passed through the control of water temperature regulation, feedstuff feeding and water quality, improve parent population growth hair
It educates, promotes the maturation of parent population sexual gland, to enhance the fertility of parent population, improve the spawning rate of red lapo-lapo, rate of fertilization and incubate
Rate.Compared with prior art, the present invention has the advantages that
(1) present invention alternately irradiate using the green light of certain wavelength and yellow light suitable first in parent fish rearing management
When duration, the grazing rate of parent population can be effectively facilitated, promote the growth and development of parent population, and after cultivating to the 2nd week~3 weeks,
The shoal of fish enhances illumination fitness, then carries out alternately irradiation using green light and feux rouges, further the activity of the promotion shoal of fish, and
It after 3 weeks, is then irradiated using single green light, guarantees the stable grazing rate of parent population, on the basis of carrying out light control, into
One step uses the corresponding stage of Three feed progress and feeds, and corresponding water temperature control, so that parent not only be effectively ensured
The food ration of fish, and promote the absorption of the nutritional ingredient of parent population, improves the growth and development quality of parent population, and then after being conducive to
The reinforced cultivating of the parent population of phase.
(2) by breeding early period in parent population, corresponding water temperature regulation, the control of feedstuff feeding and water quality have been carried out, that is,
In the November of parent population cultivation to interim water temperature regulation during March, is carried out, avoids water temperature too low and increase the disease incidence of parent population
Height, meanwhile, rotation nursing is carried out using Three feed, improves parent population immunity, guarantees the higher food ration of parent population, enhances parent population
Adaptive capacity to environment, and the sexual gland for promoting parent population is mature, enhances the fecundity of parent population, improves spawning rate and fertilization
Rate realizes the artificial propagation to the more effective scale of red lapo-lapo.
It further illustrates, it is corresponding to carry out water temperature control during light control in step (3), green using LED
When light and yellow light alternately irradiate the pond water surface, control pond water temperature is 24~25.5 DEG C；It is alternately shone using LED green light and feux rouges
When penetrating the pond water surface, control pond water temperature is 25.5~26 DEG C；The pond water surface is being irradiated using LED green light, is controlling pond water temperature
It is 26~27 DEG C.The corresponding interim water temperature control using lighting process, further ensures the food ration and rush of parent population
Into the absorption of the nutritional ingredient of parent population, the growth and development quality of parent population is improved, the rate of body weight gain of parent population significantly improves.
Further illustrate, in step (3), the light control of parent fish rearing management, in cultivation the 1st week, using LED green light with
Yellow light alternately irradiates the pond water surface, daily every kind photochromic Continuous irradiation 7 hours；In cultivation the 2nd week~3 weeks, using LED green light
The pond water surface is alternately irradiated with feux rouges, daily every kind photochromic Continuous irradiation 10 hours；At cultivation 3 weeks or more, shone using LED green light
Penetrate the pond water surface, daily Continuous irradiation 14 hours.
Further illustrate, in step (3), the wavelength of the green light of the light control of parent fish rearing management be 455~460nm,
The wavelength of yellow light is 600~650nm of wavelength of 465~475nm, feux rouges.
It further illustrates, in step (4), the pond water temperature from November to reinforced cultivating between March is at the uniform velocity risen with 1 DEG C.
Further illustrate, in step (5), the ocyodinic of injection are as follows: oxytocic hormone using human chorionic gonadtropin HCG,
Luteinizing hormone releasing hormone (LHRH), it is 3~5 μ g/kg that wherein HCG dosage, which is 400~800 IU/kg, LHRH dosages, male parent
Fish is hastened parturition using biphasic injection, is spaced 12 hours after injecting for the first time and is halved injection.
It further illustrates, in step (6), the method for collecting fertilized eggs are as follows: before the aerator of culture pond at 5~6m
Setting collection ovum net, the water flow of generation is started using aerator, and fertilized eggs are collected into collection ovum net, is prepared one in advance and is washed ovum basin,
The fishing net that 20~30 mesh are put in basin, fertilized eggs are poured into fishing net, are allowed fertilized eggs under filtering in mesh, are removed write by hand
Then net is lightly stirred clockwise washing in ovum basin with wooden dipper, rotates water in basin, then will be gathered with a hose siphonage
Bad ovum and the sundries removal of collection, repeat this method 1~2 time, are dipped net with 40~50 mesh and pick up fertilized eggs, after fresh seawater is rinsed,
Weighing.Wherein, collect the long 6m of ovum net capsule net, the high 1.2m of capsule mouth, distal opening diameter 0.4m, two sides respectively set the wing lattice of long 6m, in wing lattice
Between high 1.2m, the high 0.9m in end.
Further illustrate, the grouper compound feed include based on parts by weight 10~16 parts of fish meal, 5~9 parts of fish oil,
15~20 parts of flour, 1~5 part of yeast powder, 0.8~1 part of complex vitamin premix and 0.5~1 part of composite trace element premix
Material.Wherein, the complex vitamin premix is based on parts by weight, comprising: 1-2 parts of vitamin As, 15-30 parts of vitamin Es,
0.6-1.2 parts of vitamin Ds, 1-2 parts of vitamin Ks, 2-5 parts of riboflavin, 1-3 parts of niacin, 5-9 parts of pyridoxals, 3-4 parts of calcium pantothenates,
0.02-0.1 parts of biotins, 3-4 parts of thiamines, 0.8-2 parts of folic acid, 0.002-0.005 parts of vitamin B12s, 20-60 parts of Vitamin Cs
Sour condensed phosphate, 45-100 part inositol, 520-600 parts of cornstarch.The composite trace element premix is with parts by weight
Meter, comprising: 28-60 parts of ferrous sulfate, 45-87 parts of zinc sulfate, 0.6-2.5 parts of copper sulphate, 7-10 parts of manganese sulfates, 0.08-0.1 parts
Potassiumiodate, 0.5-1 part cobalt chloride, 0.05-0.2 parts of sodium selenites.
Compared with prior art, the beneficial effects of the present invention are: in the screening and corresponding pond culture by parent population
After the setting of condition, rank further is carried out by during parent fish rearing manages, using LED green light, yellow light and feux rouges
Section property is alternately irradiated, to substantially increase the food ration of parent population, meanwhile, it further uses Three feed and carries out the corresponding stage
Property feed, and corresponding water temperature control, and then the food ration of parent population is not only effectively ensured, and be conducive to the nutrition of parent population at
The absorption divided, improves the growth and development quality of parent population；And in breeding early period of parent population, water temperature regulation is also passed through, feed is thrown
The control with water quality is fed, the growth and development of parent population is improved, promotes the maturation of parent population sexual gland, thus enhance the fertility of parent population,
Improve spawning rate, the fertility rate and hatchability of red lapo-lapo.
Specific embodiment
In order to be best understood from the technology of the present invention content, specific embodiment is provided below, the present invention is described further.
Experimental method used in the embodiment of the present invention is conventional method unless otherwise specified.
Material used in the embodiment of the present invention, reagent etc., are commercially available unless otherwise specified.
A kind of artificial fecundation method of red lapo-lapo of embodiment 1-, includes the following steps:
(1) Juvenile stage
Female parent: select 6 more than age, red lapo-lapo that weight 3.5kg, figure are normal, disease-free, energetic is as female
Property parent；
Male parent: select 6 more than age, red lapo-lapo that weight 3.5kg, figure are normal, disease-free, energetic is as hero
Property parent；
(2) pond is cultivated
It selects to cultivate the area in the pond of parent to be 2 mu, depth of water 4m, bottom of pond is equipped with inlet and outlet, pond
Top is provided with LED light light device；Cultivation density is 100 tails/mu, female-male proportion 1:0.5；
(3) parent fish rearing management
A. light control: in cultivation the 1st week, using LED green light and the yellow light alternately irradiation pond water surface, daily every kind photochromic
Continuous irradiation 6 hours；In cultivation the 2nd week~3 weeks, the pond water surface, daily every kind of light are alternately irradiated using LED green light and feux rouges
Color Continuous irradiation 12 hours；At cultivation 3 weeks or more, the pond water surface, daily Continuous irradiation 16 hours are irradiated using LED green light；Its
In, the wavelength of green light is 445~465nm, the wavelength of yellow light is 460~480nm, 570~700nm of wavelength of feux rouges；
B. feed and feed: feed is divided into three kinds；The first is grouper compound feed, and second is fresh 1 portion of small fish
Shrimp and 5 parts of chilled trash fish, it with mass ratio is that 6:3:1 is mixed that the third, which is by grouper compound feed, chilled trash fish and spirulina powder,
It closes, the artificial soft pellet diet that system material is processed into；Wherein, grouper compound feed includes 10 parts of fish meal, 9 parts based on parts by weight
Fish oil, 15 parts of flour, 5 parts of yeast powders, 0.8 part of complex vitamin premix and 0.5 part of composite trace element premix；It is cultivating
The first feedstuff feeding was used in first week, second of feedstuff feeding is used when cultivating the 2nd week~3 weeks, at cultivation 3 weeks or more
It using the third feedstuff feeding, feeds daily feed 1 time, each feeding volume is the 2.8% of parent population weight；
C. water temperature control and water quality management: pond water temperature control is made as 23 DEG C, and water dissolved oxygen > 5mg/L is changed water 1 time for every 3 days,
Quantity of exchanged water is the 20% of pond water deep water amount, keeps water transparency in 35cm；
(4) reinforced cultivating before breeding
A. cultivate water temperature: the reinforced cultivating of parent population is carried out from November between March, and adjusting pond water temperature from November is 20
DEG C, until adjusting pond water temperature is 23 DEG C, until pond water temperature is risen to 25 DEG C when March when January；The pond of reinforced cultivating
Water temperature is at the uniform velocity risen with 1 DEG C；
B. feed and dosage in reinforced cultivating: feed is the Three feed in step (3), and Three feed rotation is used,
And every kind of feed is continuously fed 6 days, is fed within every 2 days once, each feeding volume is the 2.0% of parent population weight；
C. water quality management: changing water 1 time for every 5 days, quantity of exchanged water be pond water 22%, keep water transparency 50cm with
It is upper:
(5) artificial propagation
Start March to progressively reach sexual gland maturation by the parent population of reinforced cultivating, be observed in daily cultivating and managing, once hair
Existing parent population body colour deepens, and can carry out artificial induced spawning: before hastening parturition, by lower water temperature to 26 DEG C, parent population being caught from pond is cultivated
It rises, selects that abdomen is big, the micro- red individual of gonopore, ocyodinic is injected to parent population using intraperitoneal injection mode, injection time is early
It is injected when upper 9:00-11:00, the ocyodinic of injection are as follows: oxytocic hormone uses human chorionic gonadtropin HCG, rush corpus luteum
Hormone-releasing hormone (LHRH), it is 3 μ g/kg, male parent population, using secondary injection that wherein HCG dosage, which is 400 IU/kg, LHRH dosages,
It penetrates and hastens parturition, be spaced 12 hours after injecting for the first time and halve injection；And parent population is put back into pond immediately, carrying out the 2 of artificial induced spawning
After it, parent population starts gradually to lay eggs；
(6) collecting fertilized eggs and hatching
A. when collecting fertilized eggs, fertilized eggs is collected using collection ovum net, collection is set at 5~6m before the aerator of culture pond
Ovum net starts the water flow of generation using aerator, and fertilized eggs are collected into collection ovum net, prepares one in advance and washes ovum basin, puts in basin
The fishing net of one 20~30 mesh, fertilized eggs are poured into fishing net, are allowed fertilized eggs under filtering in mesh, are removed fishing net, so
It is lightly stirred clockwise with wooden dipper washing in ovum basin afterwards, rotates water in basin, then with a hose siphonage by aggregation
Bad ovum and sundries removal, repeat this method 1~2 time, are dipped net with 40~50 mesh and pick up fertilized eggs, after fresh seawater is rinsed, weighing；
B. fertilized eggs are put into a volume 500L, bottom equipped with hatching in the hatching barrel of drain valve, are put in hatching barrel
Set gas stone inflation, incubating oosperm condition be 24 DEG C of water temperature, salinity 28.6 ± 0.7, pH8.17, hatching density be 2,300,000 by
Smart ovum/m3, change within every 3 hours that water is primary, each quantity of exchanged water is the half of hatching barrel volume, to keep the clear of seawater in hatching barrel
Newly.
A kind of artificial fecundation method of red lapo-lapo of embodiment 2-, includes the following steps:
(1) Juvenile stage
Female parent: select 6 more than age, red lapo-lapo that weight 5.5kg, figure are normal, disease-free, energetic is as female
Property parent；
Male parent: select 6 more than age, red lapo-lapo that weight 5.5kg, figure are normal, disease-free, energetic is as hero
Property parent；
(2) pond is cultivated
It selects to cultivate the area in the pond of parent to be 3 mu, depth of water 6m, bottom of pond is equipped with inlet and outlet, pond
Top is provided with LED light light device；Cultivation density is 120 tails/mu, female-male proportion 1:1；
(3) parent fish rearing management
A. light control: in cultivation the 1st week, using LED green light and the yellow light alternately irradiation pond water surface, daily every kind photochromic
Continuous irradiation 8 hours；In cultivation the 2nd week~3 weeks, the pond water surface, daily every kind of light are alternately irradiated using LED green light and feux rouges
Color Continuous irradiation 8 hours；At cultivation 3 weeks or more, the pond water surface, daily Continuous irradiation 12 hours are irradiated using LED green light；Its
In, the wavelength of green light is 445~465nm, the wavelength of yellow light is 460~480nm, 570~700nm of wavelength of feux rouges；
B. feed and feed: feed is divided into three kinds；The first is grouper compound feed, and second is fresh 2 portions of small fish
Shrimp and 8 parts of chilled trash fish, it with mass ratio is that 6:1:2 is mixed that the third, which is by grouper compound feed, chilled trash fish and spirulina powder,
It closes, the artificial soft pellet diet that system material is processed into；The grouper compound feed includes 16 parts of fish meal, 5 parts based on parts by weight
Fish oil, 20 parts of flour, 1 part of yeast powder, 1 part of complex vitamin premix and 1 part of composite trace element premix；In cultivation first
The first feedstuff feeding is used in all, second of feedstuff feeding is used when cultivating the 2nd week~3 weeks, is used at cultivation 3 weeks or more
The third feedstuff feeding is fed feed 1 time daily, and each feeding volume is the 3.5% of parent population weight；
C. water temperature control and water quality management: it is corresponding to carry out water temperature control during light control, green using LED
When light and yellow light alternately irradiate the pond water surface, control pond water temperature is 25.5 DEG C；Pond is alternately being irradiated using LED green light and feux rouges
When the water surface of the pool, control pond water temperature is 26 DEG C；The pond water surface is being irradiated using LED green light, control pond water temperature is 27 DEG C, water-soluble
Oxygen > 5mg/L is solved, is changed within every 3 days water 1 time, quantity of exchanged water is the 23% of pond water deep water amount, keeps water transparency in 45cm；
(4) reinforced cultivating before breeding
A. cultivate water temperature: the reinforced cultivating of parent population is carried out from November between March, and adjusting pond water temperature from November is 23
DEG C, until adjusting pond water temperature is 25 DEG C, until pond water temperature is risen to 27 DEG C, the pond of reinforced cultivating when March when January
Water temperature is at the uniform velocity risen with 1 DEG C；
B. feed and dosage in reinforced cultivating: feed is the Three feed in step (3), and Three feed rotation is used,
And every kind of feed is continuously fed 8 days, is fed within every 2 days once, each feeding volume is the 2.5% of parent population weight；
C. water quality management: changing water 1 time for every 5 days, quantity of exchanged water be pond water 28%, keep water transparency 50cm with
It is upper:
(5) artificial propagation
Start March to progressively reach sexual gland maturation by the parent population of reinforced cultivating, be observed in daily cultivating and managing, once hair
Existing parent population body colour deepens, and can carry out artificial induced spawning: before hastening parturition, by lower water temperature to 28 DEG C, parent population being caught from pond is cultivated
It rises, selects that abdomen is big, the micro- red individual of gonopore, ocyodinic is injected to parent population using intraperitoneal injection mode, injection time is early
It is injected when upper 9:00-11:00, the ocyodinic of injection are as follows: oxytocic hormone uses human chorionic gonadtropin HCG, rush corpus luteum
Hormone-releasing hormone (LHRH), it is 5 μ g/kg, male parent population, using secondary injection that wherein HCG dosage, which is 800 IU/kg, LHRH dosages,
It penetrates and hastens parturition, be spaced 12 hours after injecting for the first time and halve injection；And parent population is put back into pond immediately, carrying out the 2 of artificial induced spawning
After it, parent population starts gradually to lay eggs；
(6) collecting fertilized eggs and hatching
A. when collecting fertilized eggs, fertilized eggs is collected using collection ovum net, collection is set at 5~6m before the aerator of culture pond
Ovum net starts the water flow of generation using aerator, and fertilized eggs are collected into collection ovum net, prepares one in advance and washes ovum basin, puts in basin
The fishing net of one 20~30 mesh, fertilized eggs are poured into fishing net, are allowed fertilized eggs under filtering in mesh, are removed fishing net, so
It is lightly stirred clockwise with wooden dipper washing in ovum basin afterwards, rotates water in basin, then with a hose siphonage by aggregation
Bad ovum and sundries removal, repeat this method 1~2 time, are dipped net with 40~50 mesh and pick up fertilized eggs, after fresh seawater is rinsed, weighing；
B. fertilized eggs are put into a volume 500L, bottom equipped with hatching in the hatching barrel of drain valve, are put in hatching barrel
The inflation of gas stone is set, incubating oosperm condition is 26 DEG C of water temperature, salinity 30 ± 0.7, pH8.24, and hatching density is 2,500,000 fertilizations
Ovum/m3, change within every 3 hours that water is primary, each quantity of exchanged water is the half of hatching barrel volume, to keep the pure and fresh of seawater in hatching barrel.
A kind of artificial fecundation method of red lapo-lapo of embodiment 3-, includes the following steps:
(1) Juvenile stage
Female parent: select 6 more than age, red lapo-lapo that weight 4.5kg, figure are normal, disease-free, energetic is as female
Property parent；
Male parent: select 6 more than age, red lapo-lapo that weight 4.5kg, figure are normal, disease-free, energetic is as hero
Property parent；
(2) pond is cultivated
It selects to cultivate the area in the pond of parent to be 2.5 mu, depth of water 5m, bottom of pond is equipped with inlet and outlet, pond
Top be provided with LED light light device；Cultivation density is 115 tails/mu, female-male proportion 1:0.8；
(3) parent fish rearing management
A. light control: in cultivation the 1st week, using LED green light and the yellow light alternately irradiation pond water surface, daily every kind photochromic
Continuous irradiation 7 hours；In cultivation the 2nd week~3 weeks, the pond water surface, daily every kind of light are alternately irradiated using LED green light and feux rouges
Color Continuous irradiation 10 hours；At cultivation 3 weeks or more, the pond water surface, daily Continuous irradiation 14 hours are irradiated using LED green light；Its
In, the wavelength of green light is 455~460nm, the wavelength of yellow light is 465~475nm, 600~650nm of wavelength of feux rouges；
B. feed and feed: feed is divided into three kinds；The first is grouper compound feed, and second is fresh 1 portion of small fish
Shrimp and 6 parts of chilled trash fish, it with mass ratio is that 6:2:1 is mixed that the third, which is by grouper compound feed, chilled trash fish and spirulina powder,
It closes, the artificial soft pellet diet that system material is processed into；The grouper compound feed includes 13 parts of fish meal, 8 parts based on parts by weight
Fish oil, 17 parts of flour, 3 parts of yeast powders, 0.9 part of complex vitamin premix and 0.8 part of composite trace element premix；It is cultivating
The first feedstuff feeding was used in first week, second of feedstuff feeding is used when cultivating the 2nd week~3 weeks, at cultivation 3 weeks or more
It using the third feedstuff feeding, feeds daily feed 1 time, each feeding volume is the 3.0% of parent population weight；
C. water temperature control and water quality management: it is corresponding to carry out water temperature control during light control, green using LED
When light and yellow light alternately irradiate the pond water surface, control pond water temperature is 24 DEG C；Pond is alternately being irradiated using LED green light and feux rouges
When the water surface, control pond water temperature is 25.5 DEG C；The pond water surface is being irradiated using LED green light, control pond water temperature is 26 DEG C, water-soluble
Oxygen > 5mg/L is solved, is changed within every 3 days water 1 time, quantity of exchanged water is the 22% of pond water deep water amount, keeps water transparency in 42cm；
(4) reinforced cultivating before breeding
A. cultivate water temperature: the reinforced cultivating of parent population is carried out from November between March, and adjusting pond water temperature from November is 22
DEG C, until adjusting pond water temperature is 24 DEG C, until pond water temperature is risen to 26 DEG C when March when January；The pond of reinforced cultivating
Water temperature is at the uniform velocity risen with 1 DEG C；
B. feed and dosage in reinforced cultivating: feed is the Three feed in step (3), and Three feed rotation is used,
And every kind of feed is continuously fed 7 days, is fed within every 2 days once, each feeding volume is the 2.3% of parent population weight；
C. water quality management: changing water 1 time for every 5 days, quantity of exchanged water be pond water 25%, keep water transparency 50cm with
It is upper:
(5) artificial propagation
Start March to progressively reach sexual gland maturation by the parent population of reinforced cultivating, be observed in daily cultivating and managing, once hair
Existing parent population body colour deepens, and can carry out artificial induced spawning: before hastening parturition, by lower water temperature to 27 DEG C, parent population being caught from pond is cultivated
It rises, selects that abdomen is big, the micro- red individual of gonopore, ocyodinic is injected to parent population using intraperitoneal injection mode, injection time is early
It is injected when upper 9:00-11:00, the ocyodinic of injection are as follows: oxytocic hormone uses human chorionic gonadtropin HCG, rush corpus luteum
Hormone-releasing hormone (LHRH), it is 4 μ g/kg, male parent population, using secondary injection that wherein HCG dosage, which is 600 IU/kg, LHRH dosages,
It penetrates and hastens parturition, be spaced 12 hours after injecting for the first time and halve injection；And parent population is put back into pond immediately, carrying out the 3 of artificial induced spawning
After it, parent population starts gradually to lay eggs；
(6) collecting fertilized eggs and hatching
A. when collecting fertilized eggs, fertilized eggs is collected using collection ovum net, collection is set at 5~6m before the aerator of culture pond
Ovum net starts the water flow of generation using aerator, and fertilized eggs are collected into collection ovum net, prepares one in advance and washes ovum basin, puts in basin
The fishing net of one 20~30 mesh, fertilized eggs are poured into fishing net, are allowed fertilized eggs under filtering in mesh, are removed fishing net, so
It is lightly stirred clockwise with wooden dipper washing in ovum basin afterwards, rotates water in basin, then with a hose siphonage by aggregation
Bad ovum and sundries removal, repeat this method 1~2 time, are dipped net with 40~50 mesh and pick up fertilized eggs, after fresh seawater is rinsed, weighing；
B. fertilized eggs are put into a volume 500L, bottom equipped with hatching in the hatching barrel of drain valve, are put in hatching barrel
Set gas stone inflation, incubating oosperm condition be 25 DEG C of water temperature, salinity 29.3, pH8.21, hatching density be 2,450,000 fertilized eggs/
m3, change within every 3 hours that water is primary, each quantity of exchanged water is the half of hatching barrel volume, to keep the pure and fresh of seawater in hatching barrel.
Comparative example 1- is only changed the step in (3), parent population according to a kind of artificial fecundation method of red lapo-lapo of embodiment 3
The light control of cultivating and managing, from cultivation only with LED green light irradiate the pond water surface, daily Continuous irradiation 14 hours, remaining
Step is same as Example 3.
Comparative example 2- is only changed the step in (3), parent population according to a kind of artificial fecundation method of red lapo-lapo of embodiment 3
The light control of cultivating and managing irradiates the pond water surface, daily Continuous irradiation 14 hours, remaining step only with incandescence from cultivation
It is rapid same as Example 3.
Comparative example 3- is only changed the step in (3), parent population according to a kind of artificial fecundation method of red lapo-lapo of embodiment 3
It the feed of cultivating and managing and feeds, uses and single be with mass ratio by grouper compound feed, chilled trash fish and spirulina powder
6:2:1 mixing, the artificial soft pellet diet that system material is processed into are fed, are fed daily feed 1 time, and each feeding volume is parent population
The 3.0% of weight, remaining step are same as Example 3.
Comparative example 4- is only changed the step in (4), parent population according to a kind of artificial fecundation method of red lapo-lapo of embodiment 3
Reinforced cultivating before breeding, from November between March, by the water temperature control in pond at 25 DEG C ± 0.5, remaining step is and embodiment
3 is identical.
Comparative example 5- is only changed the step in (4), parent population according to a kind of artificial fecundation method of red lapo-lapo of embodiment 3
Reinforced cultivating before breeding, use it is single by grouper compound feed, chilled trash fish and spirulina powder with mass ratio for 6:2:1
Mixing, the artificial soft pellet diet that system material is processed into are fed, and are fed within every 2 days once, and each feeding volume is 2.3%, remaining
Step is same as Example 3.
The case where artificial propagation by the red lapo-lapo in embodiment above-described embodiment 1~3 and comparative example 1~5, is carried out
It statistics and compares, the result is as follows:
According to upper table it is found that the artificial fecundation method of the red lapo-lapo by the embodiment of the present invention 1~3, can effectively improve
Parent population grazing rate, and the nutritional ingredient for being conducive to parent population absorbs, and the rate of body weight gain of parent population improves, especially in embodiment 2 and 3
In, it carries out corresponding stage using LED green light, yellow light and the interim alternately irradiation of feux rouges progress and Three feed and feeds base
On plinth, further using the interim water temperature control of lighting process, it not only ensure that the food ration of parent population and promote parent population
Nutritional ingredient absorption, and further increase the growth and development quality of parent population, the rate of body weight gain of parent population significantly improves.And
In comparative example 1, due to using only single LED green light irradiation, the grazing rate of parent population and the rate of body weight gain of parent population are reduced, and
The pond rearing survival rate and spawning rate of parent population also relative reduction；In comparative example 2, irradiated using single incandescence, often
It will affect parent population growth and development, and the survival rate of parent population caused to reduce, spawning rate, fertility rate and hatchability are substantially reduced；
In comparative example 3, during parent fish rearing management, is fed using single feed, not only drop the grazing rate of parent population
It is low, and the immunity degradation of parent population, survival rate obviously drop；In comparative example 4 and 5, in the reinforced cultivating before the breeding of parent population,
Change pond waters temperature and maintain 25 DEG C ± 0.5 always, or by the way of single feedstuff feeding, the sexual gland of parent population at
Ripe to delay, the spawning rate and hatching rate of parent population are substantially reduced, and newly hatched larvae abnormal rate significantly improves.Therefore, by this hair
The artificial fecundation method of bright red lapo-lapo, can not only effectively facilitate the grazing rate of parent population, and greatly strengthen parent population
Fertility, improves the spawning rate and rate of fertilization of parent population, and the red lapo-lapo of scale artificial culture is effectively performed in realization.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Within mind and principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (8)
1. a kind of artificial fecundation method of red lapo-lapo, characterized by the following steps:
(1) Juvenile stage
Female parent: select 6 more than age, red lapo-lapo that 3.5~5.5kg of weight, figure are normal, disease-free, energetic as
Female parent；
Male parent: select 6 more than age, red lapo-lapo that 3.5~5.5kg of weight, figure are normal, disease-free, energetic as
Male parent；
(2) pond is cultivated
It selects to cultivate the area in the pond of parent to be 2~3 mu, the depth of water is 4~6m, and bottom of pond is equipped with inlet and outlet, pond
Top be provided with LED light light device；Cultivation density is 100~120 tails/mu, and female-male proportion is 1:(0.5~1)；
(3) parent fish rearing management
A. light control: in cultivation the 1st week, using LED green light and the yellow light alternately irradiation pond water surface, daily every kind it is photochromic continuously
Irradiation 6~8 hours；In cultivation the 2nd week~3 weeks, the pond water surface, daily every kind of light are alternately irradiated using LED green light and feux rouges
Color Continuous irradiation 8~12 hours；At cultivation 3 weeks or more, the pond water surface, daily Continuous irradiation 12~16 are irradiated using LED green light
Hour；Wherein, the wavelength of green light is 445~465nm, the wavelength of yellow light is 460~480nm, 570~700nm of wavelength of feux rouges；
B. feed and feed: feed is divided into three kinds；The first is grouper compound feed, and second is fresh 1~2 part of small fish and shrimp
With 5~8 parts of chilled trash fish, the third be by grouper compound feed, chilled trash fish and spirulina powder with mass ratio for 6:(3~
1): (2~1) mixing, the artificial soft pellet diet that system material is processed into；The first feedstuff feeding is used in cultivation first week,
Second of feedstuff feeding is used when cultivating the 2nd week~3 weeks, was used the third feedstuff feeding at cultivation 3 weeks or more, is fed feeding daily
Material 1 time, each feeding volume are the 2.8%-3.5% of parent population weight；
C. water temperature control and water quality management: pond water temperature control is made as 23~27 DEG C, and water dissolved oxygen > 5mg/L is changed water 1 time for every 3 days,
Quantity of exchanged water is the 20~23% of pond water deep water amount, keeps water transparency in 35~45cm；
(4) reinforced cultivating before breeding
A. cultivate water temperature: the reinforced cultivating of parent population is carried out from November between March, and adjusting pond water temperature from November is 20~23
DEG C, until adjusting pond water temperature is 23~25 DEG C, until pond water temperature is risen to 25~27 DEG C when March when January；
B. feed and dosage in reinforced cultivating: feed is the Three feed in step (3), and Three feed rotation is used, and every
Kind feed is continuously fed 6~8 days, is fed within every 2 days once, and each feeding volume is the 2.0~2.5% of parent population weight；
C. water quality management: changing water 1 time for every 5 days, and quantity of exchanged water is the 22%~28% of pond water, keeps water transparency in 50cm
More than:
(5) artificial propagation
Start March to progressively reach sexual gland maturation by the parent population of reinforced cultivating, be observed in daily cultivating and managing, once discovery parent
Fish body discoloration is deep, can carry out artificial induced spawning: before hastening parturition, by lower water temperature to 26~27 DEG C, parent population being caught from pond is cultivated
It rises, selects that abdomen is big, the micro- red individual of gonopore, ocyodinic is injected to parent population using intraperitoneal injection mode, injection time is early
It is injected when upper 9:00-11:00, and parent population is put back into pond immediately, after carrying out 2~3 days of artificial induced spawning, parent population starts
Gradually lay eggs；
(6) collecting fertilized eggs and hatching
A. when collecting fertilized eggs, fertilized eggs is collected using collection ovum net, is rinsed with fresh seawater, removes bad ovum and sundries；
B. fertilized eggs are put into a volume 500L, bottom equipped with hatching in the hatching barrel of drain valve, gas is placed in hatching barrel
Stone inflation, incubating oosperm condition are 24-26 DEG C of water temperature, salinity 29.3 ± 0.7, pH8.21 ± 0.3, hatching density 230-
2500000 fertilized eggs/m3, change within every 3 hours that water is primary, each quantity of exchanged water is the half of hatching barrel volume, to keep in hatching barrel
Seawater it is pure and fresh.
2. a kind of artificial fecundation method of red lapo-lapo according to claim 1, it is characterised in that: in step (3), in light
It is corresponding to carry out water temperature control during according to control, when using LED green light and the yellow light alternately irradiation pond water surface, control pond
Pool water temperature is 24~25.5 DEG C；When using LED green light and the feux rouges alternately irradiation pond water surface, control pond water temperature is 25.5~
26℃；The pond water surface is being irradiated using LED green light, control pond water temperature is 26~27 DEG C.
3. a kind of artificial fecundation method of red lapo-lapo according to claim 2, it is characterised in that: in step (3), parent population
The light control of cultivating and managing alternately irradiates the pond water surface using LED green light and yellow light, daily every kind photochromic in cultivation the 1st week
Continuous irradiation 7 hours；In cultivation the 2nd week~3 weeks, the pond water surface, daily every kind of light are alternately irradiated using LED green light and feux rouges
Color Continuous irradiation 10 hours；At cultivation 3 weeks or more, the pond water surface, daily Continuous irradiation 14 hours are irradiated using LED green light.
4. a kind of artificial fecundation method of red lapo-lapo according to claim 3, it is characterised in that: in step (3), parent population
The wavelength of the green light of the light control of cultivating and managing is 455~460nm, the wavelength of yellow light is 465~475nm, the wavelength of feux rouges
600~650nm.
5. a kind of artificial fecundation method of red lapo-lapo according to claim 1, it is characterised in that: in step (4), from 11
The pond water temperature of the moon to reinforced cultivating between March is at the uniform velocity risen with 1 DEG C.
6. a kind of artificial fecundation method of red lapo-lapo according to claim 1, it is characterised in that: in step (5), injection
Ocyodinic are as follows: oxytocic hormone uses human chorionic gonadtropin HCG, luteinizing hormone releasing hormone (LHRH), wherein HCG dosage
Be 3~5 μ g/kg for 400~800IU/kg, LHRH dosage, male parent population is hastened parturition using biphasic injection, for the first time inject after between
Halved injection every 12 hours.
7. a kind of artificial fecundation method of red lapo-lapo according to claim 1, it is characterised in that: described in step (6)
The method for collecting fertilized eggs are as follows: the setting collection ovum net at 5~6m before the aerator of culture pond starts the water of generation using aerator
Stream, fertilized eggs are collected into collection ovum net, are prepared one in advance and are washed ovum basin, the fishing net of 20~30 mesh is put in basin, will be fertilized
Ovum pours into fishing net, allows fertilized eggs under filtering in mesh, removes fishing net, is then being washed in ovum basin clockwise lightly with wooden dipper
Agitation, rotates water in basin, then is removed the bad ovum of aggregation and sundries with a hose siphonage, repeats this method 1~2
It is secondary, it is dipped net with 40~50 mesh and picks up fertilized eggs, after fresh seawater is rinsed, weighing.
8. a kind of artificial fecundation method of red lapo-lapo according to claim 1, it is characterised in that: the grouper cooperation
Feed includes 10~16 parts of fish meal, 5~9 parts of fish oil, 15~20 parts of flour, 1~5 part of yeast powder, 0.8~1 based on parts by weight
Part complex vitamin premix and 0.5~1 part of composite trace element premix.
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