CN101637142B - Cross breeding method for squaliobarbus curriculus and elopichthys bambusa - Google Patents
Cross breeding method for squaliobarbus curriculus and elopichthys bambusa Download PDFInfo
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- CN101637142B CN101637142B CN2009101837328A CN200910183732A CN101637142B CN 101637142 B CN101637142 B CN 101637142B CN 2009101837328 A CN2009101837328 A CN 2009101837328A CN 200910183732 A CN200910183732 A CN 200910183732A CN 101637142 B CN101637142 B CN 101637142B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention relates to a cross breeding method for squaliobarbus curriculus and elopichthys bambusa, which belongs to the technical field of cross breeding of fish and comprises the steps of the selection of parent fish, the reinforced breeding, the preparation of the odinagogue, the artificial hastening of parturition, the artificial insemination, the artificial incubation and the cross breeding of fries. The method has the advantages of high growth speed, miscellaneous diet and strong breeding adaptability to meet the requirements of artificial large-scale cultivation. The method is simple and has ideal operability. The suitability of squaliobarbus curriculus and elopichthys bambusa is good, and the fertility rate and the hatchability respectively reach above 76.2% and 25%.
Description
Technical field
The invention belongs to the fish hybridization breeding technical field, be specifically related to a kind of Squaliobarbus curriculus and Bambusa fish cross breeding method.
Background technology
Squaliobarbus curriculus (Squaliobarbus curriculus) belongs to Cyprinidae, graining subfamily, Squaliobarbus curriculus genus, is the fresh water midwater fishes, and it is wide to distribute, and in the freshet lake of the most of province ,city and area of China distribution is arranged all; Feeding habits are assorted, are food with algae and aquatic higher plant mainly, and the hold concurrently living insect of drinking water, small fish and ovum grain etc. are through taming the artificial prescription feed of also ingesting; Individual little, growth is slow, reaches sexual maturity 2 ages, and 2-3 individuality in age is 0.5-1Kg only, and maximum individuality only is 2-2.5Kg; Fine and tender taste, delicious flavour, nutritious, for example every hectogram edible part contains protein 16.1-17.1 gram, fatty 1.3-1.6 gram, carbohydrate 2.4-3.4 gram, heat 90-92 kilocalorie, calcium 64-89 milligram, phosphorus 183-223 milligram and iron 0.6-0.9 milligram, thereby favored by the consumer.In addition, the meat of the Squaliobarbus curriculus using fresh herb of being used as medicine, have warm in and the effect of stomach, cure mainly gastric disorder causing nausea and tell food, deficiency-cold in spleen and stomach and work such as rush down at disease.
Bambusa fish (Elopichthys bambusa) belongs to Cyprinidae, graining subfamily, Bambusa genus, moves in the waters at the middle and upper levels, mainly is distributed in the rivers and lakes of China middle and lower reach of Yangtze River, is one of large-scale economic fish in rivers, the lake; The Bambusa fish is movable rapidly, and property is greedy suddenly, is violent predacious fish, and growth is fast, and 3-4 reaches sexual maturity age, one age the Bambusa fish can reach 0.5-1Kg then, two age the Bambusa fish can reach 4-5Kg, three age the Bambusa fish can reach 10-12Kg; Individual big, maximum individuality can reach 60Kg; Because of its delicious meat, and every hectogram edible part contains 1.7 milligrams of protein 18.1-20.8 gram, fatty 1.3-5.4 gram, heat 84-132 kilocalorie, calcium 11-24 milligram, phosphorus 144-203 milligram, 0.7 milligram of iron, 0.04 milligram of thiamine, 0.15 milligram in vitamin b3 and niacins, so one to being put into large-scale first-class edible fishes; But its individual character ferociousness often is used as the predator fish of aquaculture and is removed, so Bambusa fish resource amount is also fewer and feweri.
As the industry's knowledge of the reason, the fish hybridization breeding is the effective means of carrying out rearing new variety, utilize parent's advantage separately, hybridize and to obtain favourable breed characteristic with seed selection and promote breed, carry out Squaliobarbus curriculus and Bambusa fish inter-genera distant hybridization, utilize Squaliobarbus curriculus and Bambusa fish characteristics separately can obtain to have concurrently not only having the fast advantage of growth but also having the assorted adaptable good breed variety of breed of feeding habits of parents' characteristics.
Summary of the invention
Task of the present invention is to provide a kind of Squaliobarbus curriculus and Bambusa fish cross breeding method, and this method is workable, suitability good, and the filial generation of cultivating has characteristics such as growth is fast, feeding habits are assorted, breed adaptability is strong, is suitable for artificial large-scale cultivation.
Task of the present invention is finished like this, and a kind of Squaliobarbus curriculus and Bambusa fish cross breeding method may further comprise the steps:
I) parent population is selected: the female or male Squaliobarbus curriculus of choosing health and having reached sexual maturing period is as the Squaliobarbus curriculus parent population, male or the female Bambusa fish of choosing health and having reached sexual maturing period is as the Bambusa fish parent fish, and selected described Squaliobarbus curriculus parent population and Bambusa fish parent fish are dropped into respectively in separately the parent fish rearing pond;
II) reinforced cultivating: to Squaliobarbus curriculus and the Bambusa fish different feed of throwing something and feeding respectively, close on breeding period Squaliobarbus curriculus and Bambusa fish and in the pond, wash by water, and control bath rhythm, to stimulate the gonad development of Squaliobarbus curriculus and Bambusa fish;
III) ocyodinic preparation: with fish hypophysis (PG) porphyrize,, mix mutually, obtain ocyodinic with human chorionic gonadtropin (HCG) and luteinizing hormone-releasing hormone analog (LRH-A) with the thinner dilution, stand-by;
IV) artificial induced spawning: female or male Squaliobarbus curriculus and the male or female Bambusa fish that is similarly gonad development to gonad development are injected ocyodinic respectively, and control the injection volume of the dosage of hastening parturition, the spawning inducing pool that Squaliobarbus curriculus behind the injection ocyodinic and Bambusa fish are put into is separately carried out stimulation by running water, observes it and oestruses dynamically;
V) artificial insemination: in spawning inducing pool, catch Squaliobarbus curriculus and the Bambusa fish that is in the state of oestrusing, earlier the seminal fluid of Bambusa fish is clamp-oned in the container, clamp-on in the container with the ovum that is about to Squaliobarbus curriculus, perhaps earlier the seminal fluid of Squaliobarbus curriculus is clamp-oned in the container, clamp-on in the container with the ovum that is about to the Bambusa fish, and the people obtains the exomixis ovum for impelling smart ovum combination;
VI) artificial incubation: the exomixis ovum is put into the hatching of incubation vessel flowing water, obtain hybridizing fry;
VII) hybridization fish seed rearing: shift out incubation vessel when treating waist point to occur by female Squaliobarbus curriculus (♀) and male Bambusa fish (♂) or by the hybridization seedling that the female Bambusa fish (♀) and the exomixis ovum of male Squaliobarbus curriculus (♂) hybridization are hatched, the cultivation of hybridizing fry obtains the hybridization fingerling of female Squaliobarbus curriculus and male Bambusa fish or female Bambusa fish and male Squaliobarbus curriculus.
In a specific embodiment of the present invention, step I) the female Squaliobarbus curriculus (♀) that reaches sexual maturing period described in be two ages or two ages above and body weight be the above female Squaliobarbus curriculus of 1Kg, and the described male Squaliobarbus curriculus (♂) that has reached sexual maturing period be two ages or two ages above and body weight be the above male Squaliobarbus curriculus of 0.5Kg; Described male Bambusa fish (♂) that has reached sexual maturing period or female Bambusa fish (♀) be four ages or four ages above and body weight be the above Bambusa fish of 8Kg.
Squaliobarbus curriculus and the Bambusa fish different feed of throwing something and feeding respectively is meant in another specific embodiment of the present invention, Step II), to Squaliobarbus curriculus throw something and feed wheat straw or prescription feed, and to the Bambusa fish live body low value fish meal of throwing something and feeding; Described month of closing on breeding period and be meant before the breeding; Described control bath rhythm is meant the bath blanking time of shortening gradually with the approaching of breeding period, and washes by water the last week in breeding everyday.
In another specific embodiment of the present invention, described live body low value fish meal is any one or its combination in live crucian, the silver carp bighead.
Go back in the specific embodiment of the present invention, Step II I) thinner described in is that mass percent concentration is the physiological saline of 0.6-0.8%, the w/v of fish hypophysis and thinner is 1mg: 0.5-1ml, and it is that fish hypophysis 1-2mg is mixed mutually with human chorionic gonadtropin 800-1200IU and luteinizing hormone-releasing hormone analog 8-10ug that the fish hypophysis after the dilution mixes mutually with chorionic gonadotropin and luteinizing hormone-releasing hormone analog.
More of the present invention and in specific embodiment, it is outstanding and with ruddy female Squaliobarbus curriculus that the female Squaliobarbus curriculus (♀) of the gonad development step IV) is meant that body weight is expanded softness and cloacal aperture greater than 1Kg, belly, and the male Squaliobarbus curriculus (♂) of described gonad development is meant greater than 0.5Kg and the male Squaliobarbus curriculus of gently pressing spermary position adularescent seminal fluid to flow out from gonopore; The male Bambusa fish (♂) of described gonad development is meant body weight greater than 8Kg and the male Bambusa fish of gently pressing spermary position adularescent seminal fluid to flow out from gonopore, and the female Bambusa fish (♀) of described gonad development is meant that body weight expands softness and cloacal aperture is outstanding and with ruddy female Bambusa fish greater than 8Kg, belly.
In of the present invention and then specific embodiment, the injection volume of the control ocyodinic step IV) is: the injection volume to female Squaliobarbus curriculus and female Bambusa fish is heavy for the 0.5-1ml/Kg fish, and heavy for the 0.25-0.5ml/Kg fish to the injection volume of male Squaliobarbus curriculus and male Bambusa fish.
Of the present invention again more and in specific embodiment, step V) Squaliobarbus curriculus that is in the state of oestrusing described in is meant and occurs chasing mutually the Squaliobarbus curriculus of phenomenon between the Squaliobarbus curriculus, the described Bambusa fish that is in the state of oestrusing is meant between the Bambusa fish that it is to impel smart ovum in conjunction with being to add entry for clamp-oning in the same container and container gently being shaken behind the 2-4min with the ovum of the sperm of Bambusa fish and Squaliobarbus curriculus or with the ovum people of the sperm of Squaliobarbus curriculus and Bambusa fish that performance has the Bambusa fish that chases phenomenon mutually, described people.
The incubation temperature of the hatching in again of the present invention and then specific embodiment, step VI) is 22-27 ℃, and brooding time is 120-144h.
In last specific embodiment of the present invention, step VII) cultivation of hybridizing fry described in comprises that one-level is cultivated and secondary is cultivated, it is to the hybridization fry wheel animalcule of throwing something and feeding that described one-level is cultivated, the density of wheel animalcule keeps 10/ml, and at week back increase cladocera, the density of cladocera is 5/ml; Described secondary is cultivated and is meant that the body of waiting to hybridize fry carries out artificial feeding when reaching 3-4cm and tames and dociles food, and moves in the native pond and cultivate; Described cladocera is that individual lengths is the fresh water zooplankton of 0.2-1mm.
The hybridization fingerling of resulting Squaliobarbus curriculus of technical scheme provided by the invention and Bambusa fish is owing to have parents' characteristics of Squaliobarbus curriculus and Bambusa fish concurrently, thereby has the fast and feeding habits of growth and mix and culture adaptable strong point and use and satisfy artificial large-scale cultivation; Method simply has desirable operability, and Squaliobarbus curriculus and Bambusa fish suitability are good, and fertilization rate and incubation rate reach respectively more than 76.2% and 25%.
Embodiment
Embodiment 1:
I) parent population is selected: choose propagate artificially healthy anosis and the two ages entering maturing stage are above and body weight is that female Squaliobarbus curriculus more than the 1Kg is as Squaliobarbus curriculus parent population (also can claim female Squaliobarbus curriculus parent), choose four ages in the healthy anosis same entering maturing stage of propagating artificially above male Bambusa fishes as Bambusa fish parent fish (the also property held sway over a region Bambusa fish parent), selected female Squaliobarbus curriculus and selected male Bambusa fish are dropped into respectively separately in the cultivating pool, more particularly, female Squaliobarbus curriculus is dropped in the cultivating pool of female Squaliobarbus curriculus, male Bambusa fish is dropped in the cultivating pool of male Bambusa fish (breed of the branch pool), obtained by fill the post of maternal of female Squaliobarbus curriculus and the male parent filled the post of by male Bambusa fish;
Throw something and feed in the pond of the female Squaliobarbus curriculus of breed II) reinforced cultivating: to step I) as wheat straw or the wheat straw and the prescription feed of Squaliobarbus curriculus feed, in culturing the pool, male Bambusa fishpond, throw something and feed simultaneously as the low value fish of Bambusa fish meal, the low value fish is not limited to live crucian and silver carp flathead, because the breeding period of Squaliobarbus curriculus and Bambusa fish is close, basically all during the 5-7 month, therefore wash by water in the pond in two months before the breeding period of Squaliobarbus curriculus and Bambusa fish, to stimulate the gonad development of Squaliobarbus curriculus and Bambusa fish, and control bath rhythm, here the bath rhythm of being said is meant the bath blanking time of closing on and shorten gradually with breeding period, bred preceding 2 months, every first quarter moon bath was once bred previous month, bath once weekly, breed first half of the month, bath is every other day once bred the last week, bath every day once, each bath 3-4 hour, bath mode are the water pump water inlet, surpass the automatic draining of water level;
III) ocyodinic preparation: earlier fish hypophysis (PG) is ground with glass mortar, be the dilution of 0.7% physiological saline with mass percent concentration again, the w/v of PG and physiological saline is 1mg: 0.5ml, PG after the dilution is mixed by PG1mg+HCG800IU+LRH-A8ug with human chorionic gonadtropin (HCG) and luteinizing hormone-releasing hormone analog (LRH-A), obtain ocyodinic, stand-by, PG described in this step can directly act on the fish sexual gland, has the effect of inducing the fish ovulation, also have simultaneously effects such as the gonad development of promotion and the generation of promotion male and female piscinity hormone, fresh hypophysis is through acetone or alcohol dewatering and defatting and drying in the shade, place the drying receptacle sealing to preserve PG, HCG, all can buy with LRH-A from market;
IV) artificial induced spawning: to body weight greater than 1Kg, belly expands soft and cloacal aperture outstanding and the female Squaliobarbus curriculus (♀) that has a ruddy gonad development is injected by Step II I) resulting ocyodinic, specifically: carry out the thoracic cavity injection from the pectoral fin base portion of Squaliobarbus curriculus, injected dose is the body weight injection 1ml (the 1ml/Kg fish is heavy) of per kilogram Squaliobarbus curriculus, to body weight greater than 8Kg, less and male Bambusa fish (♂) injection of gently pressing the gonad development that spermary position adularescent seminal fluid flows out from gonopore of belly is equally by Step II I) ocyodinic that obtains, specifically: carry out the thoracic cavity injection from the pectoral fin portion of Bambusa fish, injected dose reduces by half for aforesaid Squaliobarbus curriculus, it is the male Bambusa fish of per kilogram body weight injection 0.5ml ocyodinic (the 0.5ml/Kg fish is heavy), put into separately spawning inducing pool respectively with having injected the female Squaliobarbus curriculus of ocyodinic and male Bambusa fish, that is to say the Squaliobarbus curriculus of having injected ocyodinic is put into the Squaliobarbus curriculus spawning inducing pool, the Bambusa fish of having injected ocyodinic is put into Bambusa fish spawning inducing pool, and carrying out miniflow water separately stimulates, the stimulation by running water mode is that the water pump water inlet carries out circulation in spawning inducing pool, observes it and oestruses dynamically;
V) artificial insemination: behind the 8h of injection ocyodinic, spawning inducing pool is observed, to be in the state of oestrusing promptly produce chase mutually state or title chase the female Squaliobarbus curriculus of phenomenon and male Bambusa fish with net from step IV) described in spawning inducing pool separately in pick up, earlier the mass percent concentration to container (present embodiment employing basin) the adding 3-5ml of the ovum of seminal fluid that is used for holding the Bambusa fish and Squaliobarbus curriculus is the physiological saline of 0.6-0.8%, again the seminal fluid of Bambusa fish is clamp-oned in the basin, also clamp-on in the basin of having clamp-oned Bambusa milt liquid with the ovum that is about to Squaliobarbus curriculus, rock basin while squeezing, and after having squeezed, again basin is jiggled 3min, impel smart ovum combination, obtain the exomixis ovum;
VI) artificial incubation: the exomixis ovum is put into the hatching of incubation vessel (present embodiment employing hatching pail) flowing water, fertilized egg keeps suspended state in hatching pail, the water inlet of hatching pail bottom, the top spilling water, gap stops with 60 Muchuang gauzes, prevent that fertilized egg and squab fry from outflowing with water, incubation temperature is 26 ℃, through the 22h incubation of membrane, again through 100-120h hatch hybridization fry, fertilization rate reached 76.2%, incubation rate reaches 25.33%;
VII) hybridization fish seed rearing: when treating waist point to occur and promptly fish glue occurs by the hybridization fry that the exomixis ovum of Squaliobarbus curriculus (♀) and Bambusa fish (♂) hybridization is hatched, shift out incubation vessel and promptly shift out hatching pail, the cultivation of hybridizing fry, the cultivation of hybridization fry comprises that one-level is cultivated and secondary is cultivated, one-level is cultivated and is carried out in cement pit, give the hybridization fry wheel animalcule of throwing something and feeding, 10/ml of wheel animalcule density, (cladocera is commonly called as red worm at 5/ml of a week back increase cladocera, a kind of individual lengths is the zooplankton of 0.2-1mm), fry to be hybridized carries out secondary when reaching 3cm (body is long) and cultivates, promptly move into the tame and docile food of artificial feeding in the native pond, in native pond routinely be that the fry rearing mode of generally using in the prior art is cultivated, obtain hybridization fingerling by female Squaliobarbus curriculus and male Bambusa fish.
Embodiment 2:
I) parent population is selected: choose propagate artificially healthy anosis and the two ages entering maturing stage are above and body weight is that male Squaliobarbus curriculus more than the 0.5Kg is as Squaliobarbus curriculus parent population (the also property held sway over a region Squaliobarbus curriculus parent), choose four ages in the healthy anosis same entering maturing stage of propagating artificially above female Bambusa fishes as Bambusa fish parent fish (also can claim female Bambusa fish parent), selected male Squaliobarbus curriculus and selected female Bambusa fish are dropped into respectively separately in the cultivating pool, more particularly, male Squaliobarbus curriculus is dropped in the cultivating pool of male Squaliobarbus curriculus, female Bambusa fish is dropped in the cultivating pool of female Bambusa fish (breed of the branch pool), obtained male parent of filling the post of by male Squaliobarbus curriculus and the female parent of filling the post of by female Bambusa fish;
Throw something and feed in the pond of the male Squaliobarbus curriculus of breed II) reinforced cultivating: to step I) as wheat straw or the wheat straw and the prescription feed of Squaliobarbus curriculus feed, in culturing the pool, female Bambusa fishpond, throw something and feed simultaneously as the low value fish of Bambusa fish meal, the low value fish is not limited to live crucian and silver carp flathead, because the breeding period of Squaliobarbus curriculus and Bambusa fish is close, basically all during the 5-7 month, therefore wash by water in the pond in month before the breeding period of Squaliobarbus curriculus and Bambusa fish, to stimulate the gonad development of Squaliobarbus curriculus and Bambusa fish, and control bath rhythm, here the bath rhythm of being said is meant the bath blanking time of closing on and shorten gradually with breeding period, bred preceding 2 months, every first quarter moon bath was once bred previous month, bath once weekly, breed first half of the month, bath is every other day once bred the last week, bath every day once, each bath 3-4 hour, bath mode are the water pump water inlet, surpass the automatic draining of water level;
III) ocyodinic preparation: earlier fish hypophysis (PG) is ground with glass mortar, be the dilution of 0.7% physiological saline with mass percent concentration again, the w/v of PG and physiological saline is 1mg: 0.6ml, PG after the dilution is mixed by PG2mg+HCG1200IU+LRH-A10ug with chorionic gonadotropin (HCG) and luteinizing hormone-releasing hormone analog (LRH-A), obtain ocyodinic, stand-by, PG described in this step can directly act on the fish sexual gland, has the effect of inducing the fish ovulation, also have simultaneously effects such as the gonad development of promotion and the generation of promotion male and female piscinity hormone, fresh hypophysis is through acetone or alcohol dewatering and defatting and drying in the shade, place the drying receptacle sealing to preserve PG, HCG, all can buy with LRH-A from market;
IV) artificial induced spawning: to body weight greater than 8Kg, belly expands soft and cloacal aperture outstanding and the female Bambusa fish (♀) that has a ruddy gonad development is injected by Step II I) resulting ocyodinic, specifically: carry out the thoracic cavity injection from the pectoral fin base portion of Bambusa fish, injected dose is the body weight injection 0.5ml (the 0.5ml/Kg fish is heavy) of per kilogram Bambusa fish, to body weight greater than 0.5Kg, less and male Squaliobarbus curriculus (♂) injection of gently pressing the gonad development that spermary position adularescent seminal fluid flows out from gonopore of belly is equally by Step II I) ocyodinic that obtains, specifically: carry out the thoracic cavity injection from the pectoral fin portion of Squaliobarbus curriculus, injected dose reduces by half for aforesaid female Bambusa fish, it is the male Squaliobarbus curriculus body weight injection of per kilogram 0.25ml ocyodinic (the 0.25ml/Kg fish is heavy), put into separately spawning inducing pool respectively with having injected the male Squaliobarbus curriculus of ocyodinic and female Bambusa fish, that is to say the male Squaliobarbus curriculus of having injected ocyodinic is put into male Squaliobarbus curriculus spawning inducing pool, the female Bambusa fish of having injected ocyodinic is put into female Bambusa fish spawning inducing pool, and carrying out miniflow water separately stimulates, miniflow water stimulation mode is that the water pump water inlet carries out circulation in spawning inducing pool, observes it and oestruses dynamically;
V) artificial insemination: behind the 8h of injection ocyodinic, spawning inducing pool is observed, to be in the state of oestrusing promptly produce chase mutually state or title chase the male Squaliobarbus curriculus of phenomenon and female Bambusa fish with net from step IV) described in spawning inducing pool separately in pick up, earlier the mass percent concentration to container (present embodiment employing basin) the adding 3-5ml of the ovum of seminal fluid that is used for holding Squaliobarbus curriculus and Bambusa fish is the physiological saline of 0.6-0.8%, again the seminal fluid of Squaliobarbus curriculus is clamp-oned in the basin, also clamp-on in the basin of having clamp-oned the Squaliobarbus curriculus seminal fluid with the ovum that is about to the Bambusa fish, rock basin while squeezing, and after having squeezed, will jiggle 3min by basin, impel smart ovum combination, obtain the exomixis ovum;
VI) artificial incubation: the exomixis ovum is put into the hatching of incubation vessel (present embodiment employing hatching pail) flowing water, fertilized egg keeps suspended state in hatching pail, the water inlet of hatching pail bottom, top spilling water, gap have 60 Muchuang gauzes to stop, prevent that fertilized egg and squab fry from outflowing with water, incubation temperature is 23 ℃, through the 26h incubation of membrane, obtains the hybridization fry through the 100-120h hatching again, fertilization rate reached 45.9%, incubation rate reaches 18.22%;
VII) hybridization fish seed rearing: when treating waist point to occur and promptly fish glue occurs by the hybridization fry that the exomixis ovum of female Bambusa fish (♀) and male Squaliobarbus curriculus (♂) hybridization is hatched, shift out incubation vessel and promptly shift out hatching pail, the cultivation of hybridizing fry, the cultivation of hybridization fry comprises that one-level is cultivated and secondary is cultivated, one-level is cultivated and is carried out in cement pit, give the hybridization fry wheel animalcule of throwing something and feeding, 10/ml of wheel animalcule density, (cladocera is commonly called as red worm at 5/ml of a week back increase cladocera, a kind of individual lengths is the zooplankton of 0.2-1mm), fry to be hybridized carries out secondary and cultivates when reaching 5cm (length), promptly move into the tame and docile food of artificial feeding in the native pond, in native pond routinely be that the fry rearing mode of generally using in the prior art is cultivated, obtain hybridization fingerling by male Squaliobarbus curriculus and female Bambusa fish.
Claims (8)
1. Squaliobarbus curriculus and Bambusa fish cross breeding method is characterized in that may further comprise the steps:
I) parent population is selected: the female or male Squaliobarbus curriculus of choosing health and having reached sexual maturing period is as the Squaliobarbus curriculus parent population, male or the female Bambusa fish of choosing health and having reached sexual maturing period is as the Bambusa fish parent fish, and selected described Squaliobarbus curriculus parent population and Bambusa fish parent fish are dropped into respectively in separately the parent fish rearing pond;
II) reinforced cultivating: to Squaliobarbus curriculus and the Bambusa fish different feed of throwing something and feeding respectively, close on breeding period Squaliobarbus curriculus and Bambusa fish and in the pond, wash by water, and control bath rhythm, to stimulate the gonad development of Squaliobarbus curriculus and Bambusa fish;
III) ocyodinic preparation: with the fish hypophysis porphyrize,, mix mutually, obtain ocyodinic with human chorionic gonadtropin and luteinizing hormone-releasing hormone analog with the thinner dilution, stand-by;
IV) artificial induced spawning: female or male Squaliobarbus curriculus and the male or female Bambusa fish that is similarly gonad development to gonad development are injected ocyodinic respectively, and control the injection volume of the dosage of hastening parturition, the spawning inducing pool that Squaliobarbus curriculus behind the injection ocyodinic and Bambusa fish are put into is separately carried out stimulation by running water, observes it and oestruses dynamically;
V) artificial insemination: in spawning inducing pool, catch Squaliobarbus curriculus and the Bambusa fish that is in the state of oestrusing, earlier the seminal fluid of Bambusa fish is clamp-oned in the container, clamp-on in the container with the ovum that is about to Squaliobarbus curriculus, perhaps earlier the seminal fluid of Squaliobarbus curriculus is clamp-oned in the container, clamp-on in the container with the ovum that is about to the Bambusa fish, and the people obtains the exomixis ovum for impelling smart ovum combination;
VI) artificial incubation: the exomixis ovum is put into the hatching of incubation vessel flowing water, obtain hybridizing fry;
VII) hybridization fish seed rearing: shift out incubation vessel when treating waist point to occur by female Squaliobarbus curriculus and male Bambusa fish or by the hybridization seedling that the exomixis ovum of female Bambusa fish and the hybridization of male Squaliobarbus curriculus is hatched, the cultivation of hybridizing fry, obtain the hybridization fingerling of female Squaliobarbus curriculus and male Bambusa fish or female Bambusa fish and male Squaliobarbus curriculus, Step II I) thinner described in is that mass percent concentration is the physiological saline of 0.6-0.8%, the w/v of fish hypophysis and thinner is 1mg: 0.5-1ml, it is that fish hypophysis 1-2mg is mixed mutually with human chorionic gonadtropin 800-1200IU and luteinizing hormone-releasing hormone analog 8-10ug that fish hypophysis after the dilution mixes mutually with chorionic gonadotropin and luteinizing hormone-releasing hormone analog, step VII) cultivation of hybridizing fry described in comprises that one-level is cultivated and secondary is cultivated, it is to the hybridization fry wheel animalcule of throwing something and feeding that described one-level is cultivated, the density of wheel animalcule keeps 10/ml, and at week back increase cladocera, the density of cladocera is 5/ml; Described secondary is cultivated and is meant that the body of waiting to hybridize fry carries out artificial feeding when reaching 3-4cm and tames and dociles food, and moves in the native pond and cultivate; Described cladocera is that individual lengths is the fresh water zooplankton of 0.2-1mm.
2. Squaliobarbus curriculus according to claim 1 and Bambusa fish cross breeding method, it is characterized in that step I) described in the female Squaliobarbus curriculus that reaches sexual maturing period be two ages or two ages above and body weight be the above female Squaliobarbus curriculus of 1Kg, and the described male Squaliobarbus curriculus that has reached sexual maturing period be two ages or two ages above and body weight be the above male Squaliobarbus curriculus of 0.5Kg; Described male Bambusa fish that has reached sexual maturing period or female Bambusa fish be four ages or four ages above and body weight be the above Bambusa fish of 8Kg.
3. Squaliobarbus curriculus according to claim 1 and Bambusa fish cross breeding method, it is characterized in that Step II) described in Squaliobarbus curriculus and the Bambusa fish different feed of throwing something and feeding respectively is meant, to Squaliobarbus curriculus throw something and feed wheat straw or prescription feed, and to the Bambusa fish live body low value fish meal of throwing something and feeding; Described month of closing on breeding period and be meant before the breeding; Described control bath rhythm is meant the bath blanking time of shortening gradually with the approaching of breeding period, and washes by water the last week in breeding everyday.
4. Squaliobarbus curriculus according to claim 3 and Bambusa fish cross breeding method is characterized in that described live body low value fish meal is any one or its combination in live crucian, the silver carp bighead.
5. Squaliobarbus curriculus according to claim 1 and Bambusa fish cross breeding method, it is characterized in that step IV) described in the female Squaliobarbus curriculus of gonad development be meant that body weight expands softness and cloacal aperture is outstanding and with ruddy female Squaliobarbus curriculus greater than 1Kg, belly, and the male Squaliobarbus curriculus of described gonad development is meant greater than 0.5Kg and the male Squaliobarbus curriculus of gently pressing spermary position adularescent seminal fluid to flow out from gonopore; The male Bambusa fish of described gonad development is meant body weight greater than 8Kg and the male Bambusa fish of gently pressing the spermary position to have the milky seminal fluid to flow out from gonopore, and the female Bambusa fish of described gonad development is meant that body weight expands softness and cloacal aperture is outstanding and with ruddy female Bambusa fish greater than 8Kg, belly.
6. Squaliobarbus curriculus according to claim 1 and Bambusa fish cross breeding method, it is characterized in that step IV) described in the injection volume of control ocyodinic be: the injection volume to female Squaliobarbus curriculus and female Bambusa fish is that the 0.5-1ml/Kg fish is heavy, and is that the 0.25-0.5ml/Kg fish is heavy to the injection volume of male Squaliobarbus curriculus and male Bambusa fish.
7. Squaliobarbus curriculus according to claim 1 and Bambusa fish cross breeding method, it is characterized in that step V) described in the Squaliobarbus curriculus that is in the state of oestrusing be meant between the Squaliobarbus curriculus and the Squaliobarbus curriculus of phenomenon to occur chasing mutually, the described Bambusa fish that is in the state of oestrusing is meant between the Bambusa fish that it is to impel smart ovum in conjunction with being to add entry for clamp-oning in the same container and container gently being shaken behind the 2-4min with the ovum of the sperm of Bambusa fish and Squaliobarbus curriculus or with the ovum people of the sperm of Squaliobarbus curriculus and Bambusa fish that performance has the Bambusa fish that chases phenomenon mutually, described people.
8. Squaliobarbus curriculus according to claim 1 and Bambusa fish cross breeding method is characterized in that step VI) described in the incubation temperature of hatching be 22-27 ℃, brooding time is 120-144h.
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| CN102124966B (en) * | 2010-12-13 | 2013-01-09 | 中国水产科学研究院淡水渔业研究中心 | Method for treating hybridized combination based on fish composite hybridization target character predicted value |
| CN103348936A (en) * | 2013-06-17 | 2013-10-16 | 句容市句容水库管理所 | Farming method for squaliobarbus curriculus |
| CN103348937B (en) * | 2013-06-17 | 2015-12-02 | 句容市句容水库管理所 | The artificial fecundation method of a kind of cute conjunctivitis trout |
| CN106719151B (en) * | 2016-12-19 | 2021-03-26 | 武汉市农业科学技术研究院水产科学研究所 | Large-scale cultivation method for squaliobarbus curriculus large-specification fingerlings |
| CN108925472B (en) * | 2018-07-02 | 2020-11-13 | 华中农业大学 | Cross breeding method for elopichthys bambusa and megalobrama amblycephala |
| CN115251010B (en) * | 2022-07-29 | 2024-01-30 | 宁波大学 | Hybridization breeding method for female salmon and male wide fin |
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