CN111109157A - Artificial spawning induction method and agent for micropterus salmoides - Google Patents

Artificial spawning induction method and agent for micropterus salmoides Download PDF

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CN111109157A
CN111109157A CN201911158058.8A CN201911158058A CN111109157A CN 111109157 A CN111109157 A CN 111109157A CN 201911158058 A CN201911158058 A CN 201911158058A CN 111109157 A CN111109157 A CN 111109157A
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fish
spawning
breeding
artificial
micropterus salmoides
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杨其彬
马振华
于刚
杨蕊
胡静
周胜杰
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Sanya Tropical Fisheries Research Institute
South China Sea Fisheries Research Institute Chinese Academy Fishery Sciences
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Sanya Tropical Fisheries Research Institute
South China Sea Fisheries Research Institute Chinese Academy Fishery Sciences
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00—Culture of aquatic animals
    • A01K61/10—Culture of aquatic animals of fish
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/47—Quinolines; Isoquinolines
    • A61K31/475—Quinolines; Isoquinolines having an indole ring, e.g. yohimbine, reserpine, strychnine, vinblastine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/04—Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
    • A61K38/08—Peptides having 5 to 11 amino acids
    • A61K38/09—Luteinising hormone-releasing hormone [LHRH], i.e. Gonadotropin-releasing hormone [GnRH]; Related peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/22—Hormones
    • A61K38/24—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g. HCG; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • A61P15/08—Drugs for genital or sexual disorders; Contraceptives for gonadal disorders or for enhancing fertility, e.g. inducers of ovulation or of spermatogenesis
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish

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Abstract

The invention provides an artificial spawning induction method and an induced spawning agent for micropterus salmoides, which comprise the following steps: (1) indoor cultivation and screening of breeding fishes: putting the micropterus salmoides fry into an indoor culture pond for culture, and screening and protecting the fry to obtain spawning-promoting fishes to be strengthened; (2) intensive maturing and culturing of breeding fish: carrying out intensified culture on the obtained spawning-promoting fingerlings to promote the sexual gland development of the fingerlings; (3) selecting and anaesthetizing breeding fishes: screening the cultured enhanced breeding fishes, and then carrying out anesthesia; (4) spawning induction of the breeding fish: injecting an oxytocic into the anesthetized breeding fish, and then putting the anesthetized breeding fish into an oxytocic pool for artificial oxytocic fertilization. The artificial spawning induction method for the micropterus salmoides adopts indoor water pool cultivation and cultivation, and high-quality post-bred fish is obtained through screening; meanwhile, the nutrition strengthening and the reinforced cultivation management are carried out on the later-prepared fingerlings, the sexual gland development of the fingerlings is promoted, the cultivation mode of the fingerlings is optimized, the spawning induction agent and the dosage of the fingerlings are optimized and determined, and the artificial ripening induction of the micropterus salmoides is realized.

Description

Artificial spawning induction method and agent for micropterus salmoides
Technical Field
The invention relates to an induced spawning method for fish, in particular to an artificial induced spawning method for micropterus salmoides, and also relates to an induced spawning agent used in the method.
Background
Lates cathayensis (Lates calcarfer) is an important economic fish in saline water culture, and lives in tropical and subtropical regions, such as south and east China sea, India, Burmese and the like. The growth speed of the micropterus salmoides is high, the micropterus salmoides can grow to the market specification within 8 months, the general growth speed is 1-2 kg/year, and the micropterus salmoides have many characteristics, so that the micropterus salmoides are suitable for coastal culture, for example, the micropterus salmoides grow well in water with high turbidity and constantly changing salinity, can adapt to rough culture and high-density culture conditions of net cages, are easy to domesticate and the like, and therefore, the micropterus salmoides become one of important objects of seawater net cage culture and pond culture in south China in recent years.
Because the natural distribution of the micropterus salmoides is limited, the micropterus salmoides are excessively fished, the ecological environment of the sea area is deteriorated, and the like, the wild natural resources of the micropterus salmoides are gradually lacked, the market supply is limited, and the sexual maturity of the micropterus salmoides is slow, so that the market demand is difficult to meet. Therefore, the development of artificial fingerling cultivation and breeding work of the acutus micropterus is not slow at all.
Disclosure of Invention
The invention aims to provide an artificial spawning induction method for micropterus salmoides, which solves the technical problems of long culture period and poor quality of fertilized eggs of the existing micropterus salmoides.
It is a further object of the invention to provide an oxytocin for use in the above method.
The purpose of the invention is realized by the following technical scheme:
an artificial spawning induction method for micropterus salmoides comprises the following steps:
(1) indoor cultivation and screening of breeding fishes: putting the micropterus salmoides fry into an indoor culture pond for culture, and screening and protecting the fry to obtain spawning-promoting fishes to be strengthened;
(2) intensive maturing and culturing of breeding fish: carrying out intensified culture on the obtained spawning-promoting fingerlings to promote the sexual gland development of the fingerlings;
(3) selecting and anaesthetizing breeding fishes: screening the cultured enhanced breeding fishes, and then carrying out anesthesia;
(4) spawning induction of the breeding fish: injecting an oxytocic into the anesthetized breeding fish, and then putting the anesthetized breeding fish into an oxytocic pool for artificial induced spawning and fertilization; the oxytocin in the oxytocin comprises chorionic gonadotropin HCG, ovulation-promoting hormone No. 2 (LRH-A2), ovulation-promoting hormone No. 3 (LRH-A3) and Reserpine (RES); the oxytocin dosage is 100-150IU/kg of chorionic gonadotropin HCG, 8-10ug/kg of ovulation-promoting hormone No. 2 (LRH-A2), 8-10ug/kg of ovulation-promoting hormone No. 3 (LRH-A3) and 3-5mg/kg of Reserpine (RES) by weight of fish; the oxytocin is prepared from oxytocin and a sodium chloride solution with the mass fraction of 0.7%; the injection dosage of the oxytocic is 3-5 mL of the injection amount of each female fish, and the injection amount of the male fish is halved.
In the application, the oxytocin increases the content of Gonadotropin (GTH) in the serum of parent fish by adding Reserpine (RES) and adopting the ratio of the oxytocin, plays a role in promoting the development and ovulation of egg cells, can enable the oxytocin to play a pharmacodynamic role in the shortest time, shortens the response time, and has obvious and stable oxytocin effect.
In some embodiments of the invention, the age of the micropterus salmoides fry is 3-5 months. The fry and offspring seed cultivation requires healthy and healthy individual with uniform fish body size and no injury.
In some embodiments of the invention, the fry and offspring seed cultivation mode adopts micro-flow water cultivation, the cultivation time is 3-5 years, and the initial stocking density of the cultivation is 150 tails/m3-200 tails/m3(ii) a Artificial puffed pellet feed is fed in the breeding process. The stocking density of the fish body is gradually adjusted along with the growth of the fish body. Further, the daily water change amount of the micro-flow water cultureIs 100-200%.
Feeding the artificial expanded pellet feed for 2 times every day, wherein the daily feeding amount is 10-15% of the weight of the fish.
Preferably, the content of crude protein in the artificially expanded pellet feed is more than or equal to 40 percent, the content of crude fat is more than or equal to 6 percent, the content of crude fiber is less than or equal to 4 percent, and the content of ash is less than or equal to 14 percent.
And (2) the seed screening and seed preservation in the step (1) is to screen out the later-stage fingerlings according to the growth condition of the seeds, then to carry out breeding and screening on the later-stage fingerlings, and the steps are repeated. The growth conditions of the fries comprise the health, vitality and size of the fish body.
Further, seed screening and seed conservation are carried out twice every year; screening 20% of fish reserved for postspawn in the first year, screening 40% of fish reserved for postspawn in the second year, and screening 50% of fish reserved for postspawn in the third year.
In the step (1), the weight of the spawning-promoting fish to be strengthened is 4kg-8kg, and no diseases exist.
In some embodiments of the invention, the intensive culture cultivation time is 2 months, and the iced fresh trash fish bait is fed during the cultivation process.
Furthermore, the bait for the iced fresh trash fish is fed for 2 times every day, and is fed for once in the morning and evening, wherein the daily feeding amount is 8% -10% of the weight of the fish.
Furthermore, the compound vitamin is added into the iced fresh trash fish bait according to the proportion of 2-5 g/kg.
In some embodiments of the invention, the intensive breeding fish is selected from mature female fish with plump and mellow abdomen, protruding oviduct and sunken vas deferens, and mature male fish with milk white semen can be extruded by lightly pressing the abdomen. The female fish and the male fish are required to have good fullness, strong physique, no diseases, complete body and strong activity.
Further, the average egg diameter of the eggs of the mature female fish is 0.4 mm.
Preferably, the ratio of the screened female fish to the screened male fish is 1: 1.
In some embodiments of the invention, the method for anesthetizing the breeding fish adopts a soaking method, and the anesthetic is eugenol.
Preferably, the concentration of eugenol in the water body is 0.5-0.8mg/L during the anesthesia process of the breeding fish, and the soaking time is 13-15 min.
In some embodiments of the invention, the injection of the oxytocin is by either intrathoracic injection or dorsal fin intramuscular injection.
Furthermore, the injection depth of the thoracic cavity is 0.5-0.6 cm, and the injection depth of dorsal fin muscle is 0.7-0.8 cm.
In the invention, artificial induced spawning and fertilization are carried out by stimulating the spawning pond with running water after the spawning of the breeding fish is induced in the induced spawning pond for 3-4 hours, so that the water flow in the water pond forms a loop, stimulating the spawning of the breeding fish with running water for 1-2 hours, and naturally fertilizing.
Further, the density of the breeding fish in the spawning inducing pond is 1-2 fish per cubic water body, the male-female ratio in the total fish number is 1: 1.
an oxytocin for artificial spawning of Perciformis latus, wherein the oxytocin comprises chorionic gonadotropin (HCG), ovulation-promoting hormone No. 2 (LRH-A2), ovulation-promoting hormone No. 3 (LRH-A3) and Reserpine (RES); the oxytocin dosage is 100-150IU/kg of chorionic gonadotropin HCG, 8-10ug/kg of ovulation-promoting hormone No. 2 (LRH-A2), 8-10ug/kg of ovulation-promoting hormone No. 3 (LRH-A3) and 3-5mg/kg of Reserpine (RES) in terms of fish body weight.
Compared with the prior art, the invention has the following beneficial effects:
(1) the artificial spawning induction method for the micropterus salmoides adopts indoor pool culture and cultivation, and periodically screens cultured individuals to obtain high-quality afterculture fish; meanwhile, the nutrition strengthening and the reinforced cultivation management are carried out on the later-prepared fingerlings, the sexual gland development of the fingerlings is promoted, the cultivation mode of the fingerlings is optimized, the spawning induction agent and the dosage of the fingerlings are optimized and determined, and the artificial ripening induction of the micropterus salmoides is realized.
(2) According to the artificial spawning induction method for the micropterus salmoides, the parent fishes are subjected to intensive maturing-promoting cultivation, the spawning rate after artificial spawning induction is higher than 70%, the average fertilization rate is higher than 85%, the obtained fertilized eggs are excellent in quality, and the hatching rate reaches 95.3%.
(3) The method of the invention has the advantages of fast sexual maturity of the parent fish, shortened culture period of the acutus micropterus, good economic benefit and larger development space.
Detailed Description
The present invention is further described below in conjunction with specific examples to better understand and implement the technical solutions of the present invention for those skilled in the art.
An artificial spawning induction method for micropterus salmoides comprises the following steps:
(1) breeding and screening of breeding fish
Purchasing 1.5 million artificially cultured micropterus salmoides fry with the age of 3-5 months, screening healthy and healthy individuals with uniform fish body size and no injury, and culturing in a culture room for 20m2The water depth of the circular water pool is 1.5-2m, the water pool is provided with middle sewage discharge, and the water quality condition of the cultivated sea area meets the requirement of the water quality standard GB 11607-89 of the marine industry. The requirement of environmental factors of the culture water quality is as follows: pH value of 2.5-8.5, dissolved oxygen>4ppm, salinity of 10-33, water temperature of 26-32 ℃, NH3<1ppm，H2S<0.3 ppm. The fry and offspring seed cultivation mode adopts micro-flow water cultivation, and the daily water change amount is 100 plus 200 percent. The initial stocking density of the culture is 150 tails/m3-200 tails/m3Gradually adjusting along with the growth of the fish body; feeding with artificial expanded pellet feed, wherein the content of crude protein in the artificial expanded pellet feed is more than or equal to 40 percent, the content of crude fat is more than or equal to 6 percent, the content of crude fiber is less than or equal to 4 percent, the ash content is less than or equal to 14 percent, the artificial expanded pellet feed is fed for 2 times a day, the daily feeding amount is 10 to 15 percent of the weight of the fish, the actual feeding amount is increased or reduced according to the influence of conditions such as weather, water temperature and the like, and the specification of the fed feed including the particle size and the content of each component is adjusted according to the size of the fish along with; meanwhile, daily management of seedling culture is enhanced, and disease control work is well done. The fish body culture is screened 2 times every year, with the individual size as an index, 20% of fish reserved for postspawn are screened in the first year, 40% of fish reserved for postspawn are screened in the second year, and 50% of fish reserved for postspawn are screened in the third year. Growing to about 2kg, and screening 150 fish tails to be used as later-bred fish. When the breeding fish is cultured to be more than 4 years old and the weight is 4kg-8kg, the spawning induction and intensified culture can be carried out on the breeding fish.
(2) Parent ripening and strengthening cultivation
Selecting 150 tails with normal body type, strong vitality and no diseases, and placing the tails at 20m2And carrying out intensified culture in a circular water pool with the water depth of 2 m. Each timeThe fresh baits are fed every day, the fresh baits are mainly fresh trash fishes and are added with compound vitamins according to the weight of the fresh baits, the baits are made into palatable size, the baits are guaranteed to be fully ingested, the baits are respectively fed for 1 time in the morning and at night, the daily feeding amount is 8 percent of the weight of the fishes, and the actual feeding amount is increased and decreased under the influence of conditions such as weather, water temperature and the like. The reinforcement is carried out 2 months before the breeding season, and the obvious abdominal swelling of the micropterus salmoides can be obviously observed after 2 months of reinforcement cultivation, so that the reinforcement can be used for spawning induction.
(3) Selection and anaesthesia of breeding fish
Selecting the breeding fish with good fullness, strong physique, no diseases, complete body and strong activity; mature female fish is full and round in belly and has protruding oviduct, female breeding fish can take eggs for inspection, the average egg diameter of the eggs is 0.4mm, mature male fish has sunken vas deferens, and milk white semen can be squeezed out by lightly pressing the belly; the ratio of male to female of the breeding fish is 1: 1; draining the water level of the culture breeding fish pond, then soaking the micropterus salmoides with anesthetic, and soaking the micropterus salmoides with eugenol with the concentration of 0.8mg/L for 13 min; and (5) carrying out the injection operation of the oxytocic when the breeding fish is in a semi-anesthesia state.
(4) Spawning induction of breeding fish
Spawning induction is carried out on the breeding fishes at the side of an indoor water pool, after the breeding fishes are anesthetized, oxytocin uses chorionic gonadotropin HCG, ovulation-promoting hormone No. 2 (LRH-A2), ovulation-promoting hormone No. 3 (LRH-A3) and Reserpine (RES), the injection dosage of the oxytocin is 100IU/kg of chorionic gonadotropin HCG, 10mg/kg of ovulation-promoting hormone No. 2 (LRH-A2), 10mg/kg of ovulation-promoting hormone No. 3 (LRH-A3) and 5mg/kg of Reserpine (RES), and the injection is an oxytocin prepared from the oxytocin and a sodium chloride solution (namely physiological saline water) with the mass fraction of 0.7 percent, the amount of the oxytocin injected into each female fish is 3-5 mL, and the amount of male fish is halved. The oxytocic prepared by the invention is added with Reserpine (RES), so that the content of Gonadotropin (GTH) in the serum of parent fish can be increased, the Gonadotropin (GTH) has promotion effect on the development and ovulation of egg cells, the effect time of the oxytocic is shortened, and the oxytocic effect is obvious and stable.
The method comprises the following steps that (1) breeding fish is injected through dorsal fin muscle, the injection depth is 0.7-0.8 cm, the breeding fish is placed into an induced spawning pond with shading measures after injection for spawning, the surrounding environment of the induced spawning pond is kept quiet in the spawning process, 1-2 fishes per cubic water body are cultured, and the male-female ratio in the total fish number is 1:1, stimulating the water flowing in the oxytocic pool after inducing for 3-4 hours by the oxytocic hormone to make the water flow in the pool form a loop, stimulating the water flowing for 1-2 hours, and naturally discharging sperm and eggs and fertilizing after inducing for 30-38 hours by the oxytocic hormone. The sperm and fertilized eggs collected after artificial induced spawning are as follows, the spawning rate of the breeding fish is higher than 70%, the average fertilization rate is higher than 85%, 65kg of fertilized eggs are collected during spawning, 56kg of high-quality fertilized eggs are obtained through screening optimization treatment, and the hatching rate of the fertilized eggs reaches 95.3%.
The above embodiment is a preferred embodiment of the invention, and in other embodiments of the invention, the oxytocic dose is 100-150IU/kg of chorionic gonadotropin HCG, 8-10ug/kg of ovulation-promoting hormone No. 2 (LRH-A2), 8-10ug/kg of ovulation-promoting hormone No. 3 (LRH-A3), and 3-5mg/kg of Reserpine (RES), and other point values in a numerical range can be selected, including end points, so that the purpose of artificial spawning induction of the acutus micropteres can be realized, and high-quality fertilized eggs can be obtained.
The above embodiments illustrate various embodiments of the present invention in detail, but the embodiments of the present invention are not limited thereto, and those skilled in the art can achieve the objectives of the present invention based on the disclosure of the present invention, and any modifications and variations based on the concept of the present invention fall within the scope of the present invention, which is defined by the claims.

Claims (10)

1. An artificial spawning induction method for micropterus salmoides is characterized by comprising the following steps:
(1) indoor cultivation and screening of breeding fishes: putting the micropterus salmoides fry into an indoor culture pond for culture, and screening and protecting the fry to obtain spawning-promoting fishes to be strengthened, the weight of which is 4kg-8 kg;
(2) intensive maturing and culturing of breeding fish: carrying out intensified culture on the obtained spawning-promoting fingerlings to promote the sexual gland development of the fingerlings;
(3) selecting and anaesthetizing breeding fishes: screening the cultured enhanced breeding fishes, and then carrying out anesthesia;
(4) spawning induction of the breeding fish: injecting an oxytocic into the anesthetized breeding fish, and then putting the anesthetized breeding fish into an oxytocic pool for artificial induced spawning and fertilization; the oxytocin in the oxytocin comprises chorionic gonadotropin HCG, ovulation-promoting hormone No. 2 (LRH-A2), ovulation-promoting hormone No. 3 (LRH-A3) and Reserpine (RES); the oxytocin dosage is 100-150IU/kg of chorionic gonadotropin HCG, 8-10ug/kg of ovulation-promoting hormone No. 2 (LRH-A2), 8-10ug/kg of ovulation-promoting hormone No. 3 (LRH-A3) and 3-5mg/kg of Reserpine (RES) by weight of fish; the oxytocin is prepared from oxytocin and a sodium chloride solution with the mass fraction of 0.7%; the injection dosage of the oxytocic is 3-5 mL of the injection amount of each female fish, and the injection amount of the male fish is halved.
2. The artificial spawning method for micropterus salmoides according to claim 1, wherein the fry of micropterus salmoides is 3-5 months old.
3. The artificial spawning induction method for the micropterus salmoides according to claim 2, wherein the fry and fry breeding mode adopts micro-flow water breeding, the breeding time is 3-5 years, and the initial stocking density of the breeding is 150 tails/m3-200 tails/m3(ii) a Artificial puffed pellet feed is fed in the breeding process.
4. The artificial spawning method for the micropterus salmoides according to claim 3, wherein the step (1) of fry screening and breed conservation is to screen out the later-bred fishes in stages according to the growth conditions of the fries, then to breed and screen the later-bred fishes, and the steps are repeated.
5. The artificial spawning method for the Perolabrax micropterus according to any one of claims 1 to 4, wherein the intensive culture cultivation time is 2 months, and the culture process is fed with the baits for the iced fresh trash fish; feeding the baits for the iced fresh trash fish for 2 times every day, and feeding the baits for the iced fresh trash fish for once every morning and evening, wherein the daily feeding amount is 8-10% of the weight of the fish.
6. The artificial spawning method of claim 5, wherein the intensive cultured adult fish is selected from mature female fish with plump and mellow abdomen, protruding oviduct and sunken vas deferens, and mature male fish with milk white semen squeezed out by lightly pressing abdomen.
7. The artificial spawning induction method for micropterus salmoides according to claim 6, wherein the method for narcotizing the micropterus salmoides adopts a soaking method, and the anesthetic is eugenol; during the anesthesia process of the breeding fish, the concentration of the eugenol in the water body is 0.5-0.8mg/L, and the soaking time is 13-15 min.
8. The artificial spawning induction method for the micropterus salmoides according to claim 7, wherein the injection of the oxytocic adopts two modes of chest injection or dorsal fin intramuscular injection; the injection depth of the thoracic cavity is 0.5-0.6 cm, and the injection depth of dorsal fin muscles is 0.7-0.8 cm.
9. The artificial spawning induction method for the micropterus salmoides according to claim 8, wherein the artificial spawning induction and fertilization are implemented by stimulating the spawning pond with running water after the spawning induction of the micropterus salmoides in the spawning induction pond is carried out for 3-4 hours, so that the water flow in the spawning induction pond forms a loop, and stimulating the spawning of the micropterus salmoides in the running water for 1-2 hours, and then carrying out natural fertilization.
10. An oxytocin for artificial spawning of Perciformis latus, wherein the oxytocin comprises chorionic gonadotropin (HCG), ovulation-promoting hormone No. 2 (LRH-A2), ovulation-promoting hormone No. 3 (LRH-A3) and Reserpine (RES); the oxytocin dosage is 100-150IU/kg of chorionic gonadotropin HCG, 8-10ug/kg of ovulation-promoting hormone No. 2 (LRH-A2), 8-10ug/kg of ovulation-promoting hormone No. 3 (LRH-A3) and 3-5mg/kg of Reserpine (RES) in terms of fish body weight.
CN201911158058.8A 2019-11-22 2019-11-22 Artificial spawning induction method and agent for micropterus salmoides Pending CN111109157A (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060169216A1 (en) * 2002-10-08 2006-08-03 Shields Robert J Isolation, culture, and use of marine copepods in aquaculture
CN107581106A (en) * 2017-09-27 2018-01-16 海南晨海水产有限公司 A kind of artificial fecundation method of platax orbicularis
CN109220903A (en) * 2018-09-26 2019-01-18 中国水产科学研究院南海水产研究所 A kind of point wing Spanish mackerel parent fish rearing and artificial ripening induced spawning method
CN110063276A (en) * 2019-04-09 2019-07-30 中国水产科学研究院南海水产研究所 A kind of method of colored perch artificial propagation and its use feed

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060169216A1 (en) * 2002-10-08 2006-08-03 Shields Robert J Isolation, culture, and use of marine copepods in aquaculture
CN107581106A (en) * 2017-09-27 2018-01-16 海南晨海水产有限公司 A kind of artificial fecundation method of platax orbicularis
CN109220903A (en) * 2018-09-26 2019-01-18 中国水产科学研究院南海水产研究所 A kind of point wing Spanish mackerel parent fish rearing and artificial ripening induced spawning method
CN110063276A (en) * 2019-04-09 2019-07-30 中国水产科学研究院南海水产研究所 A kind of method of colored perch artificial propagation and its use feed

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
区又君: "尖吻鲈的人工繁育", 《海洋与渔业》 *
周检 主编: "《池塘科学养鱼实用技术》", 30 November 2017, 四川科学技术出版社 *
陈永乐 等: "池养尖吻鲈人工繁殖的研究", 《水产科技情报》 *
雷慧僧 等: "《池塘养鱼新技术》", 30 September 2000, 金盾出版社 *

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