CN109220903A - A kind of point wing Spanish mackerel parent fish rearing and artificial ripening induced spawning method - Google Patents
A kind of point wing Spanish mackerel parent fish rearing and artificial ripening induced spawning method Download PDFInfo
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- CN109220903A CN109220903A CN201811123718.4A CN201811123718A CN109220903A CN 109220903 A CN109220903 A CN 109220903A CN 201811123718 A CN201811123718 A CN 201811123718A CN 109220903 A CN109220903 A CN 109220903A
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- 235000019688 fish Nutrition 0.000 title claims abstract description 84
- 241000251468 Actinopterygii Species 0.000 title claims abstract description 81
- 241001622901 Scomberomorus commerson Species 0.000 title claims abstract description 42
- 230000000384 rearing effect Effects 0.000 title claims abstract description 34
- 238000000034 method Methods 0.000 title claims abstract description 22
- 230000005070 ripening Effects 0.000 title claims abstract description 20
- 235000013601 eggs Nutrition 0.000 claims abstract description 30
- 230000032696 parturition Effects 0.000 claims abstract description 29
- 238000012216 screening Methods 0.000 claims abstract description 18
- 210000004681 ovum Anatomy 0.000 claims abstract description 9
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 8
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 8
- 238000009360 aquaculture Methods 0.000 claims abstract description 8
- 244000144974 aquaculture Species 0.000 claims abstract description 8
- 239000007924 injection Substances 0.000 claims abstract description 7
- 238000002347 injection Methods 0.000 claims abstract description 7
- 230000003014 reinforcing effect Effects 0.000 claims abstract description 6
- 238000012545 processing Methods 0.000 claims abstract description 5
- 230000004720 fertilization Effects 0.000 claims abstract description 4
- 238000005728 strengthening Methods 0.000 claims abstract description 4
- 230000006698 induction Effects 0.000 claims description 14
- 230000016087 ovulation Effects 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 230000037396 body weight Effects 0.000 claims description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 11
- 239000013535 sea water Substances 0.000 claims description 11
- 230000017448 oviposition Effects 0.000 claims description 8
- 235000002639 sodium chloride Nutrition 0.000 claims description 8
- 238000009395 breeding Methods 0.000 claims description 7
- 230000001488 breeding effect Effects 0.000 claims description 7
- 230000002196 ecbolic effect Effects 0.000 claims description 7
- 239000005556 hormone Substances 0.000 claims description 7
- 229940088597 hormone Drugs 0.000 claims description 7
- 239000011780 sodium chloride Substances 0.000 claims description 7
- 239000008188 pellet Substances 0.000 claims description 6
- 239000000243 solution Substances 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 5
- 201000010099 disease Diseases 0.000 claims description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 4
- 239000011782 vitamin Substances 0.000 claims description 4
- 229940088594 vitamin Drugs 0.000 claims description 4
- 241000238366 Cephalopoda Species 0.000 claims description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 3
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- 229910052760 oxygen Inorganic materials 0.000 claims description 3
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- 102000004169 proteins and genes Human genes 0.000 claims description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 3
- 230000006399 behavior Effects 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 claims 1
- 230000033822 gland development Effects 0.000 claims 1
- 230000001568 sexual effect Effects 0.000 claims 1
- 229930003231 vitamin Natural products 0.000 claims 1
- 235000013343 vitamin Nutrition 0.000 claims 1
- 150000003722 vitamin derivatives Chemical class 0.000 claims 1
- 239000003814 drug Substances 0.000 abstract 1
- 230000002710 gonadal effect Effects 0.000 abstract 1
- 230000035800 maturation Effects 0.000 abstract 1
- 238000011161 development Methods 0.000 description 4
- 230000018109 developmental process Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 230000026109 gonad development Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 241000045333 Acanthuriformes Species 0.000 description 1
- 241000801108 Ephippus orbis Species 0.000 description 1
- 241000276618 Perciformes Species 0.000 description 1
- 241000357298 Platax teira Species 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The present invention provides a kind of sharp wing Spanish mackerel parent fish rearing and artificial ripening induced spawning methods, include: (1) parent fish rearing and screening: sharp wing Spanish mackerel seed being put into aquaculture net cage and is cultivated, it selects reserve parent fish to continue to cultivate, obtains parent population to be fortified of hastening parturition;(2) parent strengthens accelerating and cultivates: parent population to be fortified of hastening parturition is carried out reinforcing cultivation;(3) parent population is hastened parturition: will carry out artificial induced spawning by strengthening the parent population injection ocyodinic that accelerating is cultivated;(4) fertilization ovum collecting and processing: the fertilized eggs in bag of laying eggs being collected, are screened to the fertilized eggs of collection, that is, completes sharp wing Spanish mackerel parent fish rearing and artificial ripening is hastened parturition.Sharp wing Spanish mackerel parent fish rearing is cultivated using cage culture in the present invention, is periodically screened to cultivation individual, is obtained high-quality reserve parent fish;Reinforce aquaculture management and fortification simultaneously, promote parent population gonadal maturation, optimizes and determine that parent population is hastened parturition medicament and dosage, is realized sharp wing Spanish mackerel artificial ripening and is hastened parturition.
Description
Technical field
The invention belongs to seawater fish culture technical fields, and in particular to a kind of point wing Spanish mackerel parent fish rearing and artificial ripening
Induced spawning method.
Background technique
Sharp wing Spanish mackerel (Platax teira) is also known as long bat, is Perciformes, Acanthuroidei, orbfish section, Spanish mackerel belongs to one
The seawater fish of the kind torrid zone and the life of Temperate Region in China offshore.It is distributed in print too sea area Arabian Sea, Indonesia to the sea of Japan
Sea area, multi-relation clustering is movable in open waters, and shape is peculiar, and especially juvenile fish is more special, can be used as ornamental fish, and adult fish is edible
With, delicious meat, economic value height.For a long time, since overfishing, sea area deterioration of the ecological environment etc. influence, sharp wing Spanish mackerel
Wild naturally increasingly deficient, the market supply is limited, it is difficult to meet the market demand.Therefore, carry out sharp wing Spanish mackerel artificial seed training
It educates and to cultivate work very urgent.
Fertilized eggs are the bases that fish fry is cultivated, and directly affect Technique in Fishes breeding process.Influence fish oosperm matter
The factor of amount includes the parent population age of a fish, nutrition condition, parent population daily management and technology of hastening parturition etc..Currently, for sharp wing Spanish mackerel
Parent fish rearing and accelerating technology of hastening parturition are not yet mature.It would therefore be highly desirable to carry out sharp wing Spanish mackerel parent fish rearing and accelerating technology of hastening parturition is excellent
Change, realize sharp wing Spanish mackerel artificial breeding, provides good seed supply for point wing Spanish mackerel aquaculture industry, promote sharp wing Spanish mackerel cultivation
Industry development, while increasing cultivation new varieties for China's sea-farming.
Summary of the invention
In view of the deficiencies of the prior art, the technical problem to be solved by the present invention is to provide a kind of sharp wing Spanish mackerel parent fish rearing and people
Work accelerating induced spawning method, this method can get sharp wing Spanish mackerel high quality fertilized egg of scophthatmus.
The present invention is that technical solution used by solving its technical problem is:
A kind of point wing Spanish mackerel parent fish rearing and artificial ripening induced spawning method, comprising the following steps:
(1) parent fish rearing and screening: sharp wing Spanish mackerel seed being put into aquaculture net cage and is cultivated, and grows feelings according to seed
Condition is screened, and reserve parent fish is selected;Reserve parent fish is continued to cultivate, obtains parent population to be fortified of hastening parturition;
(2) parent strengthens accelerating and cultivates: parent population to be fortified of hastening parturition obtained by step (1) being carried out reinforcing cultivation, promotes parent population
Gonad development;
(3) parent population is hastened parturition: step (2) being carried out artificial induced spawning by strengthening the parent population injection ocyodinic that accelerating is cultivated, are put
Enter in marine oviposition net cage oviposition bag;
(4) fertilization ovum collecting and processing: the fertilized eggs in bag of laying eggs are collected, the fertilized eggs of collection are sieved
Choosing, that is, complete sharp wing Spanish mackerel parent fish rearing and artificial ripening is hastened parturition.
Parent fish rearing sea environment factor requirements in the step (1): salinity 25 ‰ -33 ‰, dissolved oxygen > 4.0mg/L, pH
7.8-8.5,20-28 DEG C of water temperature, water quality transparency 40-50cm.
Step (1) the initial cultivation density of parent fish rearing phase seed is 200 tails/m3, as fish body growth gradually carries out
Adjustment.
It is artificial expanded pellet feed that parent fish rearing, which feeds feed, in the step (1), feeds 3 times daily, day feeding volume
For the 8%-10% of fish body weight.
Artificial expanded pellet feed gross protein value >=40%, crude fat >=6%, crude fibre≤4%, ash content≤
14%.
As fry grows up, feed specification is fed according to the adjustment of fish body size, the content including particle size and each component.
Parent population screening is to be screened stage by stage according to fish body size in the step (1);First stage fish body weight is extremely
When 0.5kg, screening 10% reserves seed for planting and makees reserve parent fish, and when second stage fish body weight is to 1.0kg, screening 20%, which is reserved seed for planting, makees standby parent
Fish, when phase III fish body weight is to 2kg, screening 50%, which is reserved seed for planting, makees reserve parent fish.
Parent population to be fortified of hastening parturition is the more than age age of a fish 4 in the step (1), and weight is in 3.0kg or more, disease-free parent population.
Strengthen cultivating 3 months in the step (2), 3 months before breeding season start, it feeds daily bait 3 times,
Each primary in the morning, afternoon and evening, day feeding volume is the 8%-10% of fish body weight.
Further, the bait is formed by chilled squid, chilled trash fish and stripped tuna ratio mixed in equal amounts by weight;Institute
It states bait and multi-vitamins is added by 2g/kg.
Sodium chloride solution (the i.e. physiology salt that ocyodinic is 0.9% by oxytocic hormone and mass fraction in the step (3)
Water) it is formulated, every fish injection volume of the ocyodinic is less than 2mL.
The oxytocic hormone is human chorionic gonadtropin HCG, ovulation induction plain No. 2 (LRH-A2) and ovulation induction are No. 3 plain
(LRH-A3)。
Further, the agent dose of hastening parturition is human chorionic gonadtropin HCG1000IU/kg, ovulation induction element 2
(LRH-A2) 10mg/kg, No. 3 (LRH-A3) 10mg/kg of ovulation induction element.
When parent population is hastened parturition in the step (3) oviposition net cage in every cube of water body 1-2 tail.
Oviposition bag is made by 80 mesh sieve tulles in the step (3), and size is consistent with aquaculture net cage, is placed on culturing net
In case, prevent fertilized eggs from dissipating off-network case.
Fertilized eggs are collected in the step (4) to be collected using 80 mesh screens.
Fertilized eggs is are placed in the fish-egg separating barrel for having seawater by fertilized eggs screening in the step (4), addition sea salt,
Seawater salinity 2-3 ‰ is improved, stirs seawater clockwise, is stood, repeats to stir and stand operation 2-3 times, be collected with 80 mesh screens
Float on the fertilized eggs on water body upper layer.
The invention has the following advantages:
(1) present invention technical optimizations such as is hastened parturition by sharp wing Spanish mackerel parent fish rearing, accelerating, can get sharp wing Spanish mackerel it is high-quality by
Smart ovum provides technical support for sharp wing Spanish mackerel seed breeding and cultivation;
(2) directed screening has been carried out to individual during sharp wing Spanish mackerel parent fish rearing in the present invention, has ensured cultivation parent population
Quality, while by fortification, ensure Fertilized quality;
(3) present invention is that marine fish culture industry development increases new varieties, to sharp wing Spanish mackerel plasm resource protection and is supported
It grows industry development and plays important impetus.
Specific embodiment
The present invention is further explained in the light of specific embodiments, so that those skilled in the art can preferably manage
The solution present invention can be simultaneously practiced, but illustrated embodiment is not as a limitation of the invention.Artificial swelling granular is raised in embodiment
Material, multi-vitamins, human chorionic gonadtropin HCG, ovulation induction plain No. 2 (LRH-A2) and ovulation induction element 3 (LRH-A3) are equal
To buy product in the market.
A kind of point wing Spanish mackerel parent fish rearing and artificial ripening induced spawning method, comprising the following steps:
(1) parent fish rearing and screening
Purchase sharp 1.5 ten thousand tail of wing Spanish mackerel seed of artificial breeding, cultivate in aquaculture net cage, cultivate sea area aquatic environment because
Son requires: salinity 25 ‰ -33 ‰, dissolved oxygen > 4.0mg/L, pH 7.8-8.5, and 20-28 DEG C of water temperature, water quality transparency 40-50cm,
Condition of water quality meets extra large industry water standard GB 11607-89 requirement.With gross protein value >=40%, crude fat >=6%, thick fibre
The artificial expanded pellet feed for tieing up≤4%, ash content≤14% is fed, and is fed day pellet 3 times, day feeding volume is fish body
The 8% of weight feeds feed specification according to the adjustment of fish body size, the content including particle size and each component as fry grows up;
Disease control work is carried out in the daily management for reinforcing seed cultivation simultaneously.Fish body is cultivated to 0.5kg or so, is with Individual Size
Index, 1500 tails of screening carry out emphasis cultivation;When fish body weight is in 1.0kg or so, carry out programmed screening, retain 300 tails into
Row cultivation;2kg or so is grown to, sieve stays 150 tails as reserve parent fish.When parent population cultivates to 4 more than age, weight 3.0kg with
On, parent population can be carried out to strengthen and hastened parturition.
(2) parent strengthens accelerating and cultivates
It selects 110 normal, energetic, disease-free tails of figure and is placed in 6m × 6m × 3m net cage and carry out reinforcing cultivation.
Chilled bait is fed daily, wherein it is 1:1:1 that chilled squid, chilled trash fish and stripped tuna, which feed ratio, and according to chilled bait
Multi-vitamins are added by 2g/kg in weight, and agreeable to the taste size is made in bait, guarantee that bait is sufficiently ingested, and each 1 time in the morning, afternoon and evening, day throws
The amount of feeding is the 8% of fish body weight.Start within 3 months before breeding season, was cultivated by 3 months, parent population gonad development can be observed obviously
It is obviously swelled to sharp wing Spanish mackerel abdomen, can be used to hasten parturition.
(3) parent population is hastened parturition
Parent population hasten parturition at sea net cage progress, oviposition bag is carried additionally in parent population aquaculture net cage, after parent population is anaesthetized, injection by
The ocyodinic that the sodium chloride solution (i.e. physiological saline) that oxytocic hormone and mass fraction are 0.9% is formulated, oxytocic hormone are adopted
With human chorionic gonadtropin HCG, ovulation induction plain No. 2 (LRH-A2) and No. 3 (LRH-A3) of ovulation induction element.Agent dose of hastening parturition is suede
Chorionic Gonadotropin HCG 1000IU/kg, ovulation induction No. 2 (LRH-A2) 10mg/kg of element, No. 3 (LRH-A3) of ovulation induction element
The amount of 10mg/kg, every fish injection ocyodinic are less than 2mL, and mass fraction is added as 0.9% according to actual amount
The amount of sodium chloride solution adjusts.Parent population is put into oviposition net cage, every cube of water body 1-2 tail, through oxytocic hormone after dorsal muscles is injected
After inducing 30-36h, parent population will discharge smart ovum naturally and be fertilized.
(4) fertilization ovum collecting and processing
Wait observe fertilized eggs in net cage of laying eggs, ovum mesh bag is received with 80 mesh sieve tulles in time, fertilized eggs are collected.It will
Fertilized eggs are placed in the conical fish-egg separating barrel for having seawater, are inflated to development of fertilized ova.Sea salt is added, improves seawater salinity
2-3 ‰ stirs seawater clockwise, stands, and repeats agitation, stands operation 2 times, and bad ovum is discharged using bottom row's method, is received with fishing net
Collection floats on the fertilized eggs on water body upper layer, as normotrophic high quality fertilized egg of scophthatmus, and the fertilized eggs after collection can be used for seed
It cultivates or sells.
110 tail parent populations are selected in the present embodiment to carry out strengthening accelerating cultivation, and parent population is carried out after the cultivation of reinforcing in 3 months
It hastens parturition, collects fertilized eggs 5.0kg, sieved excellent processing acquisition high quality fertilized egg of scophthatmus 4.2kg, hatchability of fertile eggs is up to 94.6%.
Above embodiments are only for section Example of the invention, and the scope of protection of the present invention is not limited to this, this field skill
Art personnel equivalent substitute or transformation made on the basis of present inventive concept, are within the scope of the present invention, and have
Body protection scope is subject to what claims were recorded.
Claims (10)
1. a kind of point wing Spanish mackerel parent fish rearing and artificial ripening induced spawning method, which comprises the following steps:
(1) parent fish rearing and screening: sharp wing Spanish mackerel seed is put into aquaculture net cage and is cultivated, according to seed growing state into
Row screening, selects reserve parent fish;Reserve parent fish is continued to cultivate, obtains parent population to be fortified of hastening parturition;
(2) parent strengthens accelerating and cultivates: parent population to be fortified of hastening parturition obtained by step (1) being carried out reinforcing cultivation, promotes parent population sexual gland
Development;
(3) parent population is hastened parturition: step (2) being carried out artificial induced spawning by strengthening the parent population injection ocyodinic that accelerating is cultivated, are put into sea
In upper oviposition net cage oviposition bag;
(4) fertilization ovum collecting and processing: the fertilized eggs in bag of laying eggs are collected, the fertilized eggs of collection are screened, i.e.,
It completes sharp wing Spanish mackerel parent fish rearing and artificial ripening is hastened parturition.
2. a kind of sharp wing Spanish mackerel parent fish rearing and artificial ripening induced spawning method according to claim 1, which is characterized in that described
Parent fish rearing sea area aquatic environment factor requirements in step (1): salinity 25 ‰ -33 ‰, dissolved oxygen > 4.0mg/L, pH 7.8-
8.5,20-28 DEG C of water temperature, water quality transparency 40-50cm.
3. a kind of sharp wing Spanish mackerel parent fish rearing and artificial ripening induced spawning method according to claim 1, which is characterized in that described
It is artificial expanded pellet feed that parent fish rearing, which feeds feed, in step (1), is fed daily 3 times, and day feeding volume is fish body weight
8%-10%;Artificial expanded pellet feed gross protein value >=40%, crude fat >=6%, crude fibre≤4%, ash content
≤ 14%.
4. a kind of sharp wing Spanish mackerel parent fish rearing and artificial ripening induced spawning method according to claim 1, which is characterized in that described
Parent population screening is to be screened stage by stage according to fish body size in step (1);When first stage fish body weight is to 0.5kg, screening
10%, which reserves seed for planting, makees reserve parent fish, and when second stage fish body weight is to 1.0kg, screening 20%, which is reserved seed for planting, makees reserve parent fish, the phase III
When fish body weight is to 2.0kg, screening 50%, which is reserved seed for planting, makees reserve parent fish.
5. a kind of sharp wing Spanish mackerel parent fish rearing and artificial ripening induced spawning method according to claim 1, which is characterized in that strong
It is the age of a fish 4 more than age for changing parent population of hastening parturition, and weight is in 3.0kg or more, disease-free parent population.
6. a kind of sharp wing Spanish mackerel parent fish rearing and artificial ripening induced spawning method according to claim 1, which is characterized in that described
Strengthen cultivating 3 months in step (2), 3 months before breeding season start, feed bait 3 times daily, in the morning, afternoon and evening each one
Secondary, day feeding volume is the 8%-10% of fish body weight.
7. a kind of sharp wing Spanish mackerel parent fish rearing and artificial ripening induced spawning method according to claim 6, which is characterized in that described
Bait is formed by chilled squid, chilled trash fish and stripped tuna ratio mixed in equal amounts by weight;The bait is added compound by 2g/kg
Vitamin.
8. a kind of sharp wing Spanish mackerel parent fish rearing and artificial ripening induced spawning method according to claim 1, which is characterized in that described
Ocyodinic is formulated by the sodium chloride solution that oxytocic hormone is 0.9% with mass fraction in step (3), the ocyodinic every
Fish injection volume is less than 2mL;The oxytocic hormone is human chorionic gonadtropin HCG, ovulation induction element 2 and ovulation induction are No. 3 plain.
9. a kind of sharp wing Spanish mackerel parent fish rearing and artificial ripening induced spawning method according to claim 8, which is characterized in that described
Agent dose of hastening parturition is human chorionic gonadtropin HCG 1000IU/kg, ovulation induction No. 2 10mg/kg of element, No. 3 10mg/ of ovulation induction element
kg。
10. a kind of sharp wing Spanish mackerel parent fish rearing and artificial ripening induced spawning method according to claim 1, which is characterized in that institute
Collection fertilized eggs in step (4) are stated to be collected using 80 mesh screens;Fertilized eggs screening has seawater to be placed in fertilized eggs
In fish-egg separating barrel, sea salt is added, improves seawater salinity 2-3 ‰, stirs seawater clockwise, stands, repeats to stir and stand behaviour
Make 2-3 times, the fertilized eggs for floating on water body upper layer are collected with 80 mesh screens.
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CN111109157A (en) * | 2019-11-22 | 2020-05-08 | 中国水产科学研究院南海水产研究所 | Artificial spawning induction method and agent for micropterus salmoides |
CN111440877A (en) * | 2020-02-27 | 2020-07-24 | 中国水产科学研究院南海水产研究所 | SSR multiple PCR primer for Swallow piniperis and application thereof |
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