CN109220903A - A kind of point wing Spanish mackerel parent fish rearing and artificial ripening induced spawning method - Google Patents

A kind of point wing Spanish mackerel parent fish rearing and artificial ripening induced spawning method Download PDF

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Publication number
CN109220903A
CN109220903A CN201811123718.4A CN201811123718A CN109220903A CN 109220903 A CN109220903 A CN 109220903A CN 201811123718 A CN201811123718 A CN 201811123718A CN 109220903 A CN109220903 A CN 109220903A
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China
Prior art keywords
parent
fish
spanish mackerel
parent fish
artificial
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CN201811123718.4A
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Chinese (zh)
Inventor
张殿昌
郭华阳
江世贵
刘宝锁
张楠
郭梁
朱克诚
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South China Sea Fisheries Research Institute Chinese Academy Fishery Sciences
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South China Sea Fisheries Research Institute Chinese Academy Fishery Sciences
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Priority to CN201811123718.4A priority Critical patent/CN109220903A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/10Culture of aquatic animals of fish
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Farming Of Fish And Shellfish (AREA)

Abstract

The present invention provides a kind of sharp wing Spanish mackerel parent fish rearing and artificial ripening induced spawning methods, include: (1) parent fish rearing and screening: sharp wing Spanish mackerel seed being put into aquaculture net cage and is cultivated, it selects reserve parent fish to continue to cultivate, obtains parent population to be fortified of hastening parturition;(2) parent strengthens accelerating and cultivates: parent population to be fortified of hastening parturition is carried out reinforcing cultivation;(3) parent population is hastened parturition: will carry out artificial induced spawning by strengthening the parent population injection ocyodinic that accelerating is cultivated;(4) fertilization ovum collecting and processing: the fertilized eggs in bag of laying eggs being collected, are screened to the fertilized eggs of collection, that is, completes sharp wing Spanish mackerel parent fish rearing and artificial ripening is hastened parturition.Sharp wing Spanish mackerel parent fish rearing is cultivated using cage culture in the present invention, is periodically screened to cultivation individual, is obtained high-quality reserve parent fish;Reinforce aquaculture management and fortification simultaneously, promote parent population gonadal maturation, optimizes and determine that parent population is hastened parturition medicament and dosage, is realized sharp wing Spanish mackerel artificial ripening and is hastened parturition.

Description

A kind of point wing Spanish mackerel parent fish rearing and artificial ripening induced spawning method
Technical field
The invention belongs to seawater fish culture technical fields, and in particular to a kind of point wing Spanish mackerel parent fish rearing and artificial ripening Induced spawning method.
Background technique
Sharp wing Spanish mackerel (Platax teira) is also known as long bat, is Perciformes, Acanthuroidei, orbfish section, Spanish mackerel belongs to one The seawater fish of the kind torrid zone and the life of Temperate Region in China offshore.It is distributed in print too sea area Arabian Sea, Indonesia to the sea of Japan Sea area, multi-relation clustering is movable in open waters, and shape is peculiar, and especially juvenile fish is more special, can be used as ornamental fish, and adult fish is edible With, delicious meat, economic value height.For a long time, since overfishing, sea area deterioration of the ecological environment etc. influence, sharp wing Spanish mackerel Wild naturally increasingly deficient, the market supply is limited, it is difficult to meet the market demand.Therefore, carry out sharp wing Spanish mackerel artificial seed training It educates and to cultivate work very urgent.
Fertilized eggs are the bases that fish fry is cultivated, and directly affect Technique in Fishes breeding process.Influence fish oosperm matter The factor of amount includes the parent population age of a fish, nutrition condition, parent population daily management and technology of hastening parturition etc..Currently, for sharp wing Spanish mackerel Parent fish rearing and accelerating technology of hastening parturition are not yet mature.It would therefore be highly desirable to carry out sharp wing Spanish mackerel parent fish rearing and accelerating technology of hastening parturition is excellent Change, realize sharp wing Spanish mackerel artificial breeding, provides good seed supply for point wing Spanish mackerel aquaculture industry, promote sharp wing Spanish mackerel cultivation Industry development, while increasing cultivation new varieties for China's sea-farming.
Summary of the invention
In view of the deficiencies of the prior art, the technical problem to be solved by the present invention is to provide a kind of sharp wing Spanish mackerel parent fish rearing and people Work accelerating induced spawning method, this method can get sharp wing Spanish mackerel high quality fertilized egg of scophthatmus.
The present invention is that technical solution used by solving its technical problem is:
A kind of point wing Spanish mackerel parent fish rearing and artificial ripening induced spawning method, comprising the following steps:
(1) parent fish rearing and screening: sharp wing Spanish mackerel seed being put into aquaculture net cage and is cultivated, and grows feelings according to seed Condition is screened, and reserve parent fish is selected;Reserve parent fish is continued to cultivate, obtains parent population to be fortified of hastening parturition;
(2) parent strengthens accelerating and cultivates: parent population to be fortified of hastening parturition obtained by step (1) being carried out reinforcing cultivation, promotes parent population Gonad development;
(3) parent population is hastened parturition: step (2) being carried out artificial induced spawning by strengthening the parent population injection ocyodinic that accelerating is cultivated, are put Enter in marine oviposition net cage oviposition bag;
(4) fertilization ovum collecting and processing: the fertilized eggs in bag of laying eggs are collected, the fertilized eggs of collection are sieved Choosing, that is, complete sharp wing Spanish mackerel parent fish rearing and artificial ripening is hastened parturition.
Parent fish rearing sea environment factor requirements in the step (1): salinity 25 ‰ -33 ‰, dissolved oxygen > 4.0mg/L, pH 7.8-8.5,20-28 DEG C of water temperature, water quality transparency 40-50cm.
Step (1) the initial cultivation density of parent fish rearing phase seed is 200 tails/m3, as fish body growth gradually carries out Adjustment.
It is artificial expanded pellet feed that parent fish rearing, which feeds feed, in the step (1), feeds 3 times daily, day feeding volume For the 8%-10% of fish body weight.
Artificial expanded pellet feed gross protein value >=40%, crude fat >=6%, crude fibre≤4%, ash content≤ 14%.
As fry grows up, feed specification is fed according to the adjustment of fish body size, the content including particle size and each component.
Parent population screening is to be screened stage by stage according to fish body size in the step (1);First stage fish body weight is extremely When 0.5kg, screening 10% reserves seed for planting and makees reserve parent fish, and when second stage fish body weight is to 1.0kg, screening 20%, which is reserved seed for planting, makees standby parent Fish, when phase III fish body weight is to 2kg, screening 50%, which is reserved seed for planting, makees reserve parent fish.
Parent population to be fortified of hastening parturition is the more than age age of a fish 4 in the step (1), and weight is in 3.0kg or more, disease-free parent population.
Strengthen cultivating 3 months in the step (2), 3 months before breeding season start, it feeds daily bait 3 times, Each primary in the morning, afternoon and evening, day feeding volume is the 8%-10% of fish body weight.
Further, the bait is formed by chilled squid, chilled trash fish and stripped tuna ratio mixed in equal amounts by weight;Institute It states bait and multi-vitamins is added by 2g/kg.
Sodium chloride solution (the i.e. physiology salt that ocyodinic is 0.9% by oxytocic hormone and mass fraction in the step (3) Water) it is formulated, every fish injection volume of the ocyodinic is less than 2mL.
The oxytocic hormone is human chorionic gonadtropin HCG, ovulation induction plain No. 2 (LRH-A2) and ovulation induction are No. 3 plain (LRH-A3)。
Further, the agent dose of hastening parturition is human chorionic gonadtropin HCG1000IU/kg, ovulation induction element 2 (LRH-A2) 10mg/kg, No. 3 (LRH-A3) 10mg/kg of ovulation induction element.
When parent population is hastened parturition in the step (3) oviposition net cage in every cube of water body 1-2 tail.
Oviposition bag is made by 80 mesh sieve tulles in the step (3), and size is consistent with aquaculture net cage, is placed on culturing net In case, prevent fertilized eggs from dissipating off-network case.
Fertilized eggs are collected in the step (4) to be collected using 80 mesh screens.
Fertilized eggs is are placed in the fish-egg separating barrel for having seawater by fertilized eggs screening in the step (4), addition sea salt, Seawater salinity 2-3 ‰ is improved, stirs seawater clockwise, is stood, repeats to stir and stand operation 2-3 times, be collected with 80 mesh screens Float on the fertilized eggs on water body upper layer.
The invention has the following advantages:
(1) present invention technical optimizations such as is hastened parturition by sharp wing Spanish mackerel parent fish rearing, accelerating, can get sharp wing Spanish mackerel it is high-quality by Smart ovum provides technical support for sharp wing Spanish mackerel seed breeding and cultivation;
(2) directed screening has been carried out to individual during sharp wing Spanish mackerel parent fish rearing in the present invention, has ensured cultivation parent population Quality, while by fortification, ensure Fertilized quality;
(3) present invention is that marine fish culture industry development increases new varieties, to sharp wing Spanish mackerel plasm resource protection and is supported It grows industry development and plays important impetus.
Specific embodiment
The present invention is further explained in the light of specific embodiments, so that those skilled in the art can preferably manage The solution present invention can be simultaneously practiced, but illustrated embodiment is not as a limitation of the invention.Artificial swelling granular is raised in embodiment Material, multi-vitamins, human chorionic gonadtropin HCG, ovulation induction plain No. 2 (LRH-A2) and ovulation induction element 3 (LRH-A3) are equal To buy product in the market.
A kind of point wing Spanish mackerel parent fish rearing and artificial ripening induced spawning method, comprising the following steps:
(1) parent fish rearing and screening
Purchase sharp 1.5 ten thousand tail of wing Spanish mackerel seed of artificial breeding, cultivate in aquaculture net cage, cultivate sea area aquatic environment because Son requires: salinity 25 ‰ -33 ‰, dissolved oxygen > 4.0mg/L, pH 7.8-8.5, and 20-28 DEG C of water temperature, water quality transparency 40-50cm, Condition of water quality meets extra large industry water standard GB 11607-89 requirement.With gross protein value >=40%, crude fat >=6%, thick fibre The artificial expanded pellet feed for tieing up≤4%, ash content≤14% is fed, and is fed day pellet 3 times, day feeding volume is fish body The 8% of weight feeds feed specification according to the adjustment of fish body size, the content including particle size and each component as fry grows up; Disease control work is carried out in the daily management for reinforcing seed cultivation simultaneously.Fish body is cultivated to 0.5kg or so, is with Individual Size Index, 1500 tails of screening carry out emphasis cultivation;When fish body weight is in 1.0kg or so, carry out programmed screening, retain 300 tails into Row cultivation;2kg or so is grown to, sieve stays 150 tails as reserve parent fish.When parent population cultivates to 4 more than age, weight 3.0kg with On, parent population can be carried out to strengthen and hastened parturition.
(2) parent strengthens accelerating and cultivates
It selects 110 normal, energetic, disease-free tails of figure and is placed in 6m × 6m × 3m net cage and carry out reinforcing cultivation. Chilled bait is fed daily, wherein it is 1:1:1 that chilled squid, chilled trash fish and stripped tuna, which feed ratio, and according to chilled bait Multi-vitamins are added by 2g/kg in weight, and agreeable to the taste size is made in bait, guarantee that bait is sufficiently ingested, and each 1 time in the morning, afternoon and evening, day throws The amount of feeding is the 8% of fish body weight.Start within 3 months before breeding season, was cultivated by 3 months, parent population gonad development can be observed obviously It is obviously swelled to sharp wing Spanish mackerel abdomen, can be used to hasten parturition.
(3) parent population is hastened parturition
Parent population hasten parturition at sea net cage progress, oviposition bag is carried additionally in parent population aquaculture net cage, after parent population is anaesthetized, injection by The ocyodinic that the sodium chloride solution (i.e. physiological saline) that oxytocic hormone and mass fraction are 0.9% is formulated, oxytocic hormone are adopted With human chorionic gonadtropin HCG, ovulation induction plain No. 2 (LRH-A2) and No. 3 (LRH-A3) of ovulation induction element.Agent dose of hastening parturition is suede Chorionic Gonadotropin HCG 1000IU/kg, ovulation induction No. 2 (LRH-A2) 10mg/kg of element, No. 3 (LRH-A3) of ovulation induction element The amount of 10mg/kg, every fish injection ocyodinic are less than 2mL, and mass fraction is added as 0.9% according to actual amount The amount of sodium chloride solution adjusts.Parent population is put into oviposition net cage, every cube of water body 1-2 tail, through oxytocic hormone after dorsal muscles is injected After inducing 30-36h, parent population will discharge smart ovum naturally and be fertilized.
(4) fertilization ovum collecting and processing
Wait observe fertilized eggs in net cage of laying eggs, ovum mesh bag is received with 80 mesh sieve tulles in time, fertilized eggs are collected.It will Fertilized eggs are placed in the conical fish-egg separating barrel for having seawater, are inflated to development of fertilized ova.Sea salt is added, improves seawater salinity 2-3 ‰ stirs seawater clockwise, stands, and repeats agitation, stands operation 2 times, and bad ovum is discharged using bottom row's method, is received with fishing net Collection floats on the fertilized eggs on water body upper layer, as normotrophic high quality fertilized egg of scophthatmus, and the fertilized eggs after collection can be used for seed It cultivates or sells.
110 tail parent populations are selected in the present embodiment to carry out strengthening accelerating cultivation, and parent population is carried out after the cultivation of reinforcing in 3 months It hastens parturition, collects fertilized eggs 5.0kg, sieved excellent processing acquisition high quality fertilized egg of scophthatmus 4.2kg, hatchability of fertile eggs is up to 94.6%.
Above embodiments are only for section Example of the invention, and the scope of protection of the present invention is not limited to this, this field skill Art personnel equivalent substitute or transformation made on the basis of present inventive concept, are within the scope of the present invention, and have Body protection scope is subject to what claims were recorded.

Claims (10)

1. a kind of point wing Spanish mackerel parent fish rearing and artificial ripening induced spawning method, which comprises the following steps:
(1) parent fish rearing and screening: sharp wing Spanish mackerel seed is put into aquaculture net cage and is cultivated, according to seed growing state into Row screening, selects reserve parent fish;Reserve parent fish is continued to cultivate, obtains parent population to be fortified of hastening parturition;
(2) parent strengthens accelerating and cultivates: parent population to be fortified of hastening parturition obtained by step (1) being carried out reinforcing cultivation, promotes parent population sexual gland Development;
(3) parent population is hastened parturition: step (2) being carried out artificial induced spawning by strengthening the parent population injection ocyodinic that accelerating is cultivated, are put into sea In upper oviposition net cage oviposition bag;
(4) fertilization ovum collecting and processing: the fertilized eggs in bag of laying eggs are collected, the fertilized eggs of collection are screened, i.e., It completes sharp wing Spanish mackerel parent fish rearing and artificial ripening is hastened parturition.
2. a kind of sharp wing Spanish mackerel parent fish rearing and artificial ripening induced spawning method according to claim 1, which is characterized in that described Parent fish rearing sea area aquatic environment factor requirements in step (1): salinity 25 ‰ -33 ‰, dissolved oxygen > 4.0mg/L, pH 7.8- 8.5,20-28 DEG C of water temperature, water quality transparency 40-50cm.
3. a kind of sharp wing Spanish mackerel parent fish rearing and artificial ripening induced spawning method according to claim 1, which is characterized in that described It is artificial expanded pellet feed that parent fish rearing, which feeds feed, in step (1), is fed daily 3 times, and day feeding volume is fish body weight 8%-10%;Artificial expanded pellet feed gross protein value >=40%, crude fat >=6%, crude fibre≤4%, ash content ≤ 14%.
4. a kind of sharp wing Spanish mackerel parent fish rearing and artificial ripening induced spawning method according to claim 1, which is characterized in that described Parent population screening is to be screened stage by stage according to fish body size in step (1);When first stage fish body weight is to 0.5kg, screening 10%, which reserves seed for planting, makees reserve parent fish, and when second stage fish body weight is to 1.0kg, screening 20%, which is reserved seed for planting, makees reserve parent fish, the phase III When fish body weight is to 2.0kg, screening 50%, which is reserved seed for planting, makees reserve parent fish.
5. a kind of sharp wing Spanish mackerel parent fish rearing and artificial ripening induced spawning method according to claim 1, which is characterized in that strong It is the age of a fish 4 more than age for changing parent population of hastening parturition, and weight is in 3.0kg or more, disease-free parent population.
6. a kind of sharp wing Spanish mackerel parent fish rearing and artificial ripening induced spawning method according to claim 1, which is characterized in that described Strengthen cultivating 3 months in step (2), 3 months before breeding season start, feed bait 3 times daily, in the morning, afternoon and evening each one Secondary, day feeding volume is the 8%-10% of fish body weight.
7. a kind of sharp wing Spanish mackerel parent fish rearing and artificial ripening induced spawning method according to claim 6, which is characterized in that described Bait is formed by chilled squid, chilled trash fish and stripped tuna ratio mixed in equal amounts by weight;The bait is added compound by 2g/kg Vitamin.
8. a kind of sharp wing Spanish mackerel parent fish rearing and artificial ripening induced spawning method according to claim 1, which is characterized in that described Ocyodinic is formulated by the sodium chloride solution that oxytocic hormone is 0.9% with mass fraction in step (3), the ocyodinic every Fish injection volume is less than 2mL;The oxytocic hormone is human chorionic gonadtropin HCG, ovulation induction element 2 and ovulation induction are No. 3 plain.
9. a kind of sharp wing Spanish mackerel parent fish rearing and artificial ripening induced spawning method according to claim 8, which is characterized in that described Agent dose of hastening parturition is human chorionic gonadtropin HCG 1000IU/kg, ovulation induction No. 2 10mg/kg of element, No. 3 10mg/ of ovulation induction element kg。
10. a kind of sharp wing Spanish mackerel parent fish rearing and artificial ripening induced spawning method according to claim 1, which is characterized in that institute Collection fertilized eggs in step (4) are stated to be collected using 80 mesh screens;Fertilized eggs screening has seawater to be placed in fertilized eggs In fish-egg separating barrel, sea salt is added, improves seawater salinity 2-3 ‰, stirs seawater clockwise, stands, repeats to stir and stand behaviour Make 2-3 times, the fertilized eggs for floating on water body upper layer are collected with 80 mesh screens.
CN201811123718.4A 2018-09-26 2018-09-26 A kind of point wing Spanish mackerel parent fish rearing and artificial ripening induced spawning method Pending CN109220903A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110692551A (en) * 2019-09-23 2020-01-17 中国水产科学研究院南海水产研究所 Pond-level ecological breeding method for young ostriches acutifolius
CN111109157A (en) * 2019-11-22 2020-05-08 中国水产科学研究院南海水产研究所 Artificial spawning induction method and agent for micropterus salmoides
CN111440877A (en) * 2020-02-27 2020-07-24 中国水产科学研究院南海水产研究所 SSR multiple PCR primer for Swallow piniperis and application thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2390428B1 (en) * 2011-04-15 2013-10-01 Universidad De Almería PREPARED FOR PROBIOTIC BACTERIA FOR ORAL ADMINISTRATION TO CULTIVATED FISH BASED ON THE ENCAPSULATION IN ALGINATE HYDROGELS.
CN103782939A (en) * 2014-02-17 2014-05-14 威海圣航水产科技有限公司 Manual induced spawning method for platichthys stellatus parental fishes
TW201741978A (en) * 2016-05-20 2017-12-01 古高爾控股有限公司 Eel aquaculture system using ICT
CN107581106A (en) * 2017-09-27 2018-01-16 海南晨海水产有限公司 A kind of artificial fecundation method of platax orbicularis

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2390428B1 (en) * 2011-04-15 2013-10-01 Universidad De Almería PREPARED FOR PROBIOTIC BACTERIA FOR ORAL ADMINISTRATION TO CULTIVATED FISH BASED ON THE ENCAPSULATION IN ALGINATE HYDROGELS.
CN103782939A (en) * 2014-02-17 2014-05-14 威海圣航水产科技有限公司 Manual induced spawning method for platichthys stellatus parental fishes
TW201741978A (en) * 2016-05-20 2017-12-01 古高爾控股有限公司 Eel aquaculture system using ICT
CN107581106A (en) * 2017-09-27 2018-01-16 海南晨海水产有限公司 A kind of artificial fecundation method of platax orbicularis

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
王明学: "《罗非鱼养殖》", 31 August 1997, 科学技术文献出版社 *
雷霁霖: "《海水鱼类养殖理论与技术》", 31 March 2005, 中国农业出版社 *
黎祖福 等: "《南方海水鱼类繁殖与养殖技术》", 30 April 2006, 海洋出版社 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110692551A (en) * 2019-09-23 2020-01-17 中国水产科学研究院南海水产研究所 Pond-level ecological breeding method for young ostriches acutifolius
CN111109157A (en) * 2019-11-22 2020-05-08 中国水产科学研究院南海水产研究所 Artificial spawning induction method and agent for micropterus salmoides
CN111440877A (en) * 2020-02-27 2020-07-24 中国水产科学研究院南海水产研究所 SSR multiple PCR primer for Swallow piniperis and application thereof

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