CN114831060A - Method for cultivating Micropterus salmoides twice gonadal maturation within one year - Google Patents

Method for cultivating Micropterus salmoides twice gonadal maturation within one year Download PDF

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CN114831060A
CN114831060A CN202210703253.XA CN202210703253A CN114831060A CN 114831060 A CN114831060 A CN 114831060A CN 202210703253 A CN202210703253 A CN 202210703253A CN 114831060 A CN114831060 A CN 114831060A
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micropterus salmoides
fish
water
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parent
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潘正军
朱传坤
常国亮
赵海涛
王辉
丁怀宇
吴楠
李才生
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Huaiyin Normal University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
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    • A01K61/10Culture of aquatic animals of fish
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K63/00Receptacles for live fish, e.g. aquaria; Terraria
    • A01K63/06Arrangements for heating or lighting in, or attached to, receptacles for live fish
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K63/00Receptacles for live fish, e.g. aquaria; Terraria
    • A01K63/06Arrangements for heating or lighting in, or attached to, receptacles for live fish
    • A01K63/065Heating or cooling devices
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    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • A23K10/22Animal feeding-stuffs from material of animal origin from fish
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/80Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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Abstract

The invention relates to the field of aquaculture technology and fishery independent innovation, and discloses a method for cultivating micropterus salmoides twice in one year, which comprises the steps of parent selection temporary cultivation, cultivation water temperature reduction in summer, cultivation water temperature increase in autumn and winter, illumination time adjustment, parent nutrition supplement adjustment, gonad maturity index calculation and the like, induces the micropterus salmoides gonads to grow mature in an out-of-season manner, and meets the requirement of out-of-season seedling on the serious shortage of parents. By means of a water temperature adjusting technology, a natural illumination simulation technology and a nutrition strengthening technology, the water temperature, illumination and nutrition requirements of natural seasonal changes on fish gonad development are simulated, gonads of the micropterus salmoides are induced to mature for the second time in one year, the purpose of providing enough parents needed by out-of-season seedling raising is achieved, and support is provided for expanding the culture scale of the micropterus salmoides and realizing adult fish culture in the same year.

Description

Method for cultivating Micropterus salmoides twice gonadal maturation within one year
Technical Field
The invention relates to the field of aquaculture technology and fishery independent innovation, in particular to a method for cultivating micropterus salmoides twice in gonad maturation within one year.
Background
Micropterus salmoides (C. Merrill.)Micropterus salmoides) The large-mouth black bass is also called carnivorous fish with wide temperature adaptability, the artificial culture of the large-mouth black bass is rapidly developed in recent years, the total yield of the large-mouth black bass is more than 60 million tons in the whole country in 2020, the price is maintained to be more than 10 yuan/500 g, the profitability is larger than or equal to 50 percent, and the large-mouth black bass is higher than other common cultured freshwater fishes (the national fishery statistical yearbook of China, 2021). The weight can be increased by 400 plus 500g in one year, the growth is fast, the catching is easy, the price is high, the culture period is shorter than that of four major Chinese carps, and the feed is popular among farmers. The meat is solid, the meat flavor is fragrant, no muscle pricks exist, and the living body is well popular among consumers, especially young people, and the market prospect is wide. But the water temperature in winter and spring in the area north of the Yangtze river is low and cannot reach the water temperature required by growth of the Micropterus salmoides (C)>10 ℃), the local artificial breeding offspring seeds can be put into a pond after the outdoor water temperature rises to about 15 ℃ after 4 months, the problems that the culture specification is small and the offspring seeds cannot be listed in the current year exist in the situations, and the culture period is longer, the risk is increased and the cost is higher when the offspring seeds are cultured in the second year. The weever in California stops eating and growing in winter, and culturists have the habit of drying and sunning the pond again, so that a fish-out peak exists in autumn and winter, and the price is reduced to some extent. Therefore, out-of-season breeding is becoming a new trend, and out-of-season fry breeding is a bottleneck restricting the development of the micropterus salmoides breeding industry.
In the first 4 th to the middle 5 th of the year, the water temperature is 18-26 ℃ which is the breeding season of Micropterus salmoides, and the gonads of 6-10 months are degenerated and can not be bred. Inducing the gonads of the Micropterus salmoides to mature for the second time in one year, and providing enough parents needed by out-of-season seedling culture becomes an urgent problem to be solved.
In the reproductive cycle of teleost, the gonadal development of female individuals is disturbed by various factors, while male individuals are generally rarely subjected to environmental restrictions. The proliferation of the oocytes in the ovaries of female fish, egg yolk accumulation, maturation of the oocytes and their ovulation and oviposition, each of which must be accurate and coordinated to ensure that the fertilising oocytes are produced at the most appropriate time. The formation of cyclic changes in the gonads is influenced by environmental and seasonal variations.
The fish is a temperature-variable animal, and the metabolic strength, gonad development and maturity of the fish are closely related to the water temperature. The temperature of each fish starting to lay eggs in a certain area is constant, and the arrival of the laying temperature is a powerful signal of the laying behavior. Temperature is one of the most important ecological factors and can have a direct effect on all levels of the hypothalamic-pituitary-gonadal axis. The temperature effect in the gonad development and maturation and ovulation processes of teleost is shown as follows: 1) directly acts on gonads, influences the activity and action of some enzymes and hormones; 2) influence the sensitivity of the gonads to gonadotropins; 3) affecting the secretion and synthesis of gonadotropin from the pituitary. The effect of temperature on spawning is significant, with each fish having its own unique spawning temperature range, which is narrow. The different requirements on temperature conditions in the process of gonad cell development of various fishes are the expression of genetic conservation adapted to the living conditions in phylogenetic development.
The illumination cycle is an important environmental factor for regulating the fish reproduction cycle, and the illumination is transmitted to the hypothalamus through the pineal gland and eyes and reaches the pituitary gland to influence the release of gonadotropin, thereby starting a series of endocrine mechanisms, and regulating the gonadal development and ovulation. Generally, increasing the duration of light or continuing light exposure at the beginning of the reproductive cycle advances oocyte maturation and ovulation. Four major Chinese carps all belong to long-day fishes, namely spawning in the long-day illumination period from spring to summer. The salmon and trout belong to short-day fishes, and lay eggs in the short-day period from autumn to winter. It is presumed that the micropterus salmoides should belong to the long-day fish species.
Nutrition is the essential material basis for gonad development. The parent fish culture is enhanced before the breeding period, aiming at storing necessary nutrition to ensure the normal proceeding of reproduction. The nutrition affects not only the development and maturation of the gonads, but also the fertilization rate, the quality of the newly hatched larvae and the like.
Under natural conditions, the development of fish gonads, the maturation of oocytes and the ovulation of fish are all performed under the action of environmental factors, and the change of the environmental factors is a starting factor of the physiological processes. By comprehensively controlling and changing the external environmental factors, the reproductive cycle of the fish can be regulated and controlled. The invention combines the artificial temperature control and the illumination period to simulate the seasonal variation, carries out nutrition enhancement through bait fish culture and feeding, and solves the problem of neck clamping lack of parent fish during the off-season seedling culture of the micropterus salmoides culture industry in California.
Disclosure of Invention
The purpose of the invention is as follows: aiming at the problems in the prior art, the invention provides a method for cultivating the gonad of the micropterus salmoides twice in one year, which comprises the steps of selecting the parent fish after spawning to temporarily cultivate after spring fry cultivation, and artificially inducing the gonad of the micropterus salmoides to secondarily mature in one year in a circulating water temperature-controllable fry cultivating pool, so that large batches of synchronously mature parents can be obtained, and large-scale anti-season fries can be obtained.
In summer in 6-7 months under natural conditions, winter low temperature is simulated in 8-9 months to perform overwintering treatment on parent fishes, short-day simulated irradiation is used as an auxiliary, spring water temperature rise is simulated in the beginning of 10-11 months, long-day simulated irradiation is used as an auxiliary, bait fish feeding is performed on the parent fishes to perform nutrition enhancement, growth and maturation of gonads of the micropterus salmoides are promoted, gonad maturation indexes are used as indexes to judge the growth conditions of the gonads of the micropterus salmoides, artificial spawning is performed on the parent fishes in the middle and last days of 11 months, and large-batch synchronously mature parents are obtained to obtain large-scale off-season fry.
The technical scheme is as follows: the invention provides a method for cultivating two times of gonad maturation of micropterus salmoides in one year, which comprises the following steps: s1: and (3) breeding bait fish: artificially propagating megalobrama amblycephala in the middle and last ten days of April, artificially propagating silver carp and bighead carp in May, artificially propagating grass carp fry in June, and breeding into summer fingerlings to be used as bait fish for enriching the nutrition of Micropterus salmoides; s2: parent fish selection and temporary rearing: after the normal reproduction of the Micropterus salmoides in spring is finished in 4-5 months every year, putting healthy parent Micropterus salmoides into a separate pond or a net cage according to the proportion of male and female 1:1 for temporary culture, feeding twice in the morning and at the evening according to 3-5% of the weight of the fishes every day, feeding the Micropterus salmoides with special feed 5:30-6:30 in the morning and feeding bait fish 5:30-6:30 in the evening; irradiating natural light, performing conventional management, and performing summer exposure under natural conditions; s3: overwintering simulation: naturally heating and intensively culturing in months 6 and 7, transferring the temporarily cultured parent fishes of the micropterus salmoides into a seedling pond when the parent fishes enter month 8, keeping the water temperature consistent with the outdoor temperature of 23-25 ℃, and setting the illumination time for 13 hours every day; after adapting for 3-5 days, injecting deep river water, deep reservoir water or well water treated in the water storage tank into the seedling raising tank, reducing the temperature of the seedling raising tank to 12-15 ℃ at the rate of 0.5-1.5 ℃ per day, and finishing after 10-13 days; meanwhile, the illumination time per day is shortened from 13 hours to 10 hours, and is shortened by about 15-18 minutes per day; during the period, the feeding amount is reduced from 3-5% of the fish body weight to 1.5-2%, and the feeding frequency and the bait variety are unchanged; maintaining the water temperature, the illumination time and the feeding amount to the bottom of 9 months; s4: simulation of breeding season: in 10 months, raising the temperature of the seedling raising pond to 21-23 ℃ at the rate of 0.5-1.5 ℃ per day, and finishing the process for 8-10 days; meanwhile, the illumination time per day is prolonged from 10 hours to 11 hours, and is prolonged by about 8-10 minutes per day; during the period, the feeding amount is increased from 1.5-2% of the weight of the fish to 3-5%, and the feeding amount of the live bait fish is properly increased; s5: artificially hastening parturition after gonad maturity: randomly extracting 2-3 female fishes every 4-6 days in 11 months, weighing, dissecting and taking ovaries, calculating the gonad maturity index according to the weight/full weight of the ovaries, and obtaining batch parent fishes spawning simultaneously by artificial induced spawning when the index is more than or equal to 5.8 percent for large-scale out-of-season seedling culture.
Furthermore, the water storage pool is sealed and provided with a top cover, can insulate heat and preserve heat, and has the volume of 1/3-1/5 of the seedling raising pool; the water inlet is provided with a filter device and a flow type ultraviolet sterilizer, the extracted deep river water, deep reservoir water or well water is input through a heat insulation pipeline, and the outlet is provided with an air energy heat pump to control the water temperature.
Preferably, the breeding pond is closed by a plastic film before parent micropterus salmoides are transplanted into the breeding pond, the distance between the ground and the ceiling is 2.5-3.0m, and every 30m 2 One 40W white light fluorescent tube is arranged.
Preferably, the method of artificial induction comprises the following steps: artificially hastening parturition by injecting oxytocin for 1-2 times; when 2 injections are adopted, the two injections are separated by 9 to 10 hours.
Preferably, the nursery pond is provided with an indoor circulating water system.
Has the advantages that: the invention aims to solve the technical problems that simulated fishes overwinter in summer and autumn and simulated fishes in autumn and winter are heated up to spring and enter the spawning season, and meanwhile, the special requirements of gonad development of micropterus salmoides on nutrition are met.
The method comprises the steps of selecting spawned parent fishes for temporary culture after spring fry culture, beginning summer in 6-7 months under natural conditions, simulating winter at low temperature in 8-9 months to perform overwintering treatment on the parent fishes, assisting short-day simulated irradiation, simulating spring water temperature rise in 10-11 months and assisting long-day simulated irradiation, meanwhile feeding bait fishes to the parent fishes for nutrition enhancement, promoting gonad development and maturation of the Micropterus salmoides, judging gonad development conditions of the Micropterus salmoides parent fishes by taking gonad maturation indexes as indexes, and performing artificial spawning on the parent fishes in the middle and the last ten days of 11 months to realize artificial induction of secondary gonad maturation of the Micropterus salmoides within one year, so that large-scale anti-season fish fries can be obtained.
One, simulation of overwintering and simulation of breeding season save energy, and season change is obvious
(1) In the prior art, an air conditioner compressor and an aquaculture constant-temperature heat pump are mostly used for cooling in summer, long-term startup is needed, and energy consumption is high. The invention uses deep river water, deep reservoir water or well water to cool the water in the seedling raising pond in summer, thereby saving a large amount of energy. The water temperature of deep river water, deep reservoir water or well water in winter is higher than that of tap water and surface water, and energy can be saved even if the deep river water, the deep reservoir water or the well water needs to be heated by an air energy heat pump. (2) A white light fluorescent lamp is arranged above the breeding pond, the illumination time of each day is accurately controlled, the sunshine duration changing in seasons under the natural state is simulated, parent fishes are stimulated, and sunshine response is generated. Through the treatment of adjusting the water temperature and the illumination time length, the season change of summer, winter and spring can be completed within half a year, and the method is suitable for the rhythm of physiological adjustment of the micropterus salmoides.
Secondly, the nutrient enrichment meets the requirements of gonadal development
There is a prevailing statement in the micropterus salmoides farming industry that "micropterus salmoides are naturally diabetic". Since micropterus salmoides, a carnivorous fish, do not use carbohydrates as an energy source in the long-term evolution process, but mainly use proteins and fats as an energy source, have poor ability to utilize carbohydrates, have a small number of insulin receptors and a low affinity compared to omnivorous fish. However, starch is inevitably existed in the production process of the expanded feed, the excessive level of carbohydrate in the feed can inhibit the growth of fish bodies, and objective conditions are created for the generation of diabetes of micropterus salmoides, so that the micropterus salmoides are susceptible to hepatobiliary syndrome. Therefore, the requirement of gonad development of the micropterus salmoides on nutrition such as protein, fat and the like cannot be met by simple feed feeding, and the problem can be effectively solved by carrying out nutrition enhancement through bait fish cultivation and feeding. There are companies or farmers who use iced fish or surimi as a nutrition-fortifying material, but there are the following problems: (1) as the country implements the ecological priority strategy, the policies of fishing in the quarter and fishing prohibition in rivers and lakes are realized, the prices of the iced fish and the surimi are high, and the cost is high. The cost can be effectively reduced by cultivating bait fish by oneself. (2) The residual bait is easy to cause the pollution of the aquaculture water body. (3) In summer, the temperature is high, the iced fresh fish or minced fillet is possibly rotten, and the risk of bringing germs is caused. The live baits are used as bait fishes in the application, and the Micropterus salmoides can be exercised when snatching, so that the physical fitness is enhanced, the risk of infection of germs is avoided, and multiple purposes are achieved.
Detailed Description
The present invention will be described in detail with reference to specific examples.
Embodiment 1:
the method for cultivating the micropterus salmoides twice in gonad maturation within one year is implemented according to the following steps:
1. parent fish selection and temporary rearing: although the Micropterus salmoides can be matured in one year, the Micropterus salmoides are not suitable for being used as breeding parents, and 2-3-year-old large individuals are selected as parent fishes. After the normal reproduction of the weever in California in spring is finished in 4-5 months every year, healthy and active parent fish with fresh body color and no trauma is selected, the weight of the parent fish is 0.8-1.1 kg/tail, the parent fish is put into a pond with a clear pond according to the ratio of male and female 1:1 for temporary culture, and the parent fish is fed twice in the morning and at night according to 3-5% of the weight of the fish every day. If no suitable pond exists, 60-90m is used 2 And (4) cage culture. The water depth is more than 2m, thereby ensuring that summer heat is avoided when the summer is hot.
2. And (3) bait fish cultivation: the megalobrama amblycephala is artificially propagated in the middle and last ten days of April, the silver carp and the bighead carp are artificially propagated in May, and the grass carp fry is artificially propagated in June and cultivated into summer fingerlings to be used as bait fish for enriching the nutrition of the Micropterus salmoides. The growth rate of the bait fish can be controlled by increasing and decreasing the feeding times and the feeding amount, and the bait fish with proper size is kept to be supplied.
3. Summer long-day feeding and management: the special feed for Micropterus salmoides is fed at 6:00 in the morning in 6-7 months, and bait fish is fed at 6:00 in the evening, wherein the feeding amount is 3-5% of the weight of the fish. Irradiating natural light and performing conventional management.
4. Simulating overwintering: when the method enters 8 months, temporarily-cultured micropterus salmoides are pulled to a net to avoid fish body injury, and the fish is quickly transferred into a seedling raising pond with an indoor circulating water system, the water temperature is kept consistent with that of the outdoor water (23-25 ℃), and the illumination is carried out for 13 hours/day. After adapting for 3-5 days, injecting filtered and disinfected deep river water, deep reservoir water or well water into the nursery pond, reducing the temperature of the nursery pond to 12-15 ℃ at the rate of 1 ℃ per day, and completing the cultivation after 10-13 days. Meanwhile, the illumination time per day is shortened from 13 hours to 10 hours, and is shortened by about 15-18 minutes per day. During the period, the feeding amount is reduced from 3-5% of the fish body weight, and the feeding frequency and the bait type are unchanged. Maintaining the water temperature (12-15 deg.C), illumination (10 hr) and feeding amount (1.5-2%) to the end of 9 months.
5. Simulation of breeding season: and (5) in 10 months, starting the air energy heat pump, raising the water temperature of the seedling raising pool to 21-23 ℃ at the rate of 1 ℃ per day, and finishing the process for 8-10 days. Meanwhile, the illumination time per day is prolonged from 10 hours to 11 hours, and is prolonged by about 8-10 minutes per day. During the period, the feeding amount is increased from 1.5-2% to 3-5% of the fish body weight, the abdominal change of the female fish is observed every day, and the feeding amount of the bait fish can be properly increased according to the development condition of the ovary of the female fish.
6. Gonad maturity index was examined and recorded: randomly extracting 2-3 female fish every 5 days in 11 months, weighing, dissecting and taking ovaries, calculating gonad maturity index according to weight/full weight of the ovaries, and when the index is more than or equal to 5.8%, the gonad maturity index can be used for artificial spawning induction.
7. Artificial hastening parturition: the mature female fish is thicker and shorter in body, darker in body color, smooth in gill cover part, round in pectoral fin, enlarged and soft in hind abdomen part, obvious in ovary outline and prominent in reproductive pore swelling. The mature male fish is long and narrow in body, dark and bright in body color, slightly rough in gill cover, long and narrow in chest fin, concave in genital hole, and small in abdomen compared with female fish. The artificial induced spawning can adopt 1 injection or 2 injections. The oxytocic can be used alone or in combination. When 2 injections are used, the intervals are generally 9 to 10 hours. In general, the male fish only needs to be injected with the oxytocic once. Therefore, the mass parent fish which lay eggs simultaneously can be obtained, and strong support is provided for realizing large-scale out-of-season fry breeding.
Embodiment 2:
this embodiment is substantially the same as embodiment 1, and is different only in that deep river water, deep reservoir water, or deep well water is not used in the simulated overwintering step, and the water temperature is controlled directly using the air-source heat pump.
Embodiment 3:
this embodiment is substantially the same as embodiment 1, and is different only in that natural lighting is performed every day in the breeding workshop in the simulated overwintering step, and the lighting time is shortened by adopting an artificial shielding door and window to reach the required lighting time.
Embodiment 4:
this embodiment is substantially the same as embodiment 1, and is different only in that deep river water, deep reservoir water, or deep well water is not used in the breeding season simulation step, and the water temperature is controlled directly using the air-source heat pump.
Embodiment 5:
this embodiment is substantially the same as embodiment 1, except that the self-farming bait fish is not used in the breeding season simulation step, but the self-farming bait fish is fed using purchased iced fresh fish or minced fillet. .
Comparative example 1:
this comparative example is substantially the same as embodiment 1, and is different only in that the bait fish is not fed to the temporarily-cultured parent fish, and the bait fish is fed to the parent fish exclusively for micropterus salmoides.
Comparative example 2:
the comparative example adopts common natural conditions for feeding, a certain sample is taken for temporary culture at the end of the spring breeding season, the breeding workshop is not switched in 8 months, the special feed for the micropterus salmoides is used for feeding in the whole process, the parent cultured in 11 months and the factory is simultaneously sampled, and the gonad maturity index and the survival rate are calculated.
The following table is made for the results of the incubation methods of the above embodiments 1 to 5 and comparative examples 1 and 2:
example 2019:
Figure 877361DEST_PATH_IMAGE001
the embodiment in 2020:
Figure DEST_PATH_IMAGE003
as can be seen from the above table, the gonad maturation indexes required for propagation can be achieved according to embodiments 1, 2, 4 and 5, but embodiment 1 has the highest survival rate and the lowest cost. Embodiment 3 demonstrates the regulatory effect of light on gonad development in cases of insufficient light intensity, which results in insufficient gonad development maturity. Embodiment 5 saves labor costs, but increases feed costs and decreases survival rates, which is not cost effective. Comparative example 1 the whole course of using the special feed for micropterus salmoides can not satisfy the nutrition required by the ovary development of parent fish, the ovary development is insufficient, and the induced spawning can not be carried out. The parent gonad of comparative example 2 did not enter the reproductive stage morphology at all and could not be used as a reproductive parent.
The above embodiments are merely illustrative of the technical concepts and features of the present invention, and the purpose of the embodiments is to enable those skilled in the art to understand the contents of the present invention and implement the present invention, and not to limit the protection scope of the present invention. All equivalent changes and modifications made according to the spirit of the present invention should be covered within the protection scope of the present invention.

Claims (5)

1. A method for cultivating Micropterus salmoides twice in gonad maturation within one year is characterized by comprising the following steps:
s1: and (3) breeding bait fish: artificially propagating megalobrama amblycephala in the middle and last ten days of April, artificially propagating silver carp and bighead carp in May, artificially propagating grass carp fry in June, and breeding into summer fingerlings to be used as bait fish for enriching the nutrition of Micropterus salmoides;
s2: parent fish selection and temporary rearing: after the normal reproduction of the Micropterus salmoides in spring is finished in 4-5 months every year, putting healthy parent Micropterus salmoides into a separate pond or a net cage according to the proportion of male and female 1:1 for temporary culture, feeding twice in the morning and at the evening according to 3-5% of the weight of the fishes every day, feeding the Micropterus salmoides with special feed 5:30-6:30 in the morning and feeding bait fish 5:30-6:30 in the evening; irradiating natural light, performing conventional management, and performing summer exposure under natural conditions;
s3: overwintering simulation: naturally heating and intensively culturing in months 6 and 7, transferring the temporarily cultured parent fishes of the micropterus salmoides into a seedling pond when the parent fishes enter month 8, keeping the water temperature consistent with the outdoor temperature of 23-25 ℃, and setting the illumination time for 13 hours every day; after adapting for 3-5 days, injecting deep river water, deep reservoir water or well water treated in the water storage tank into the seedling raising tank, reducing the temperature of the seedling raising tank to 12-15 ℃ at the rate of 0.5-1.5 ℃ per day, and finishing after 10-13 days; meanwhile, the illumination time per day is shortened from 13 hours to 10 hours, and is shortened by about 15-18 minutes per day; during the period, the feeding amount is reduced from 3-5% of the fish body weight to 1.5-2%, and the feeding frequency and the bait variety are unchanged; maintaining the water temperature, the illumination time and the feeding amount to the bottom of 9 months;
s4: simulation of breeding season: in 10 months, raising the temperature of the seedling raising pond to 21-23 ℃ at the rate of 0.5-1.5 ℃ per day, and finishing the process for 8-10 days; meanwhile, the illumination time per day is prolonged from 10 hours to 11 hours, and is prolonged by about 8-10 minutes per day; during the period, the feeding amount is increased from 1.5-2% of the weight of the fish to 3-5%, and the feeding amount of the live bait fish is properly increased;
s5: artificially hastening parturition after gonad maturity: randomly extracting 2-3 female fishes every 4-6 days in 11 months, weighing, dissecting and taking ovaries, calculating the gonad maturity index according to the weight/full weight of the ovaries, and obtaining batch parent fishes spawning simultaneously by artificial induced spawning when the index is more than or equal to 5.8 percent for large-scale out-of-season seedling culture.
2. The method for cultivating micropterus salmoides twice gonadal maturation in one year according to claim 1, wherein the water storage tank is sealed, provided with a top cover, can insulate heat and preserve heat, and has a volume of 1/3-1/5 of a nursery pond; the water inlet is provided with a filter device and a flow type ultraviolet sterilizer, the extracted deep river water, deep reservoir water or well water is input through a heat insulation pipeline, and the outlet is provided with an air energy heat pump to control the water temperature.
3. The method as claimed in claim 1, wherein the rearing pond is closed by a plastic film before the parent micropterus salmoides are transplanted into the rearing pond, the distance between the ground and the ceiling is 2.5-3.0m, and every 30m 2 One 40W white light fluorescent tube is arranged.
4. The method for cultivating weever twice gonadal maturation in one year as claimed in claim 1, wherein the artificial induced spawning method comprises the following steps:
artificially hastening parturition by injecting oxytocin for 1-2 times; when 2 injections are adopted, the two injections are separated by 9 to 10 hours.
5. The method as claimed in any one of claims 1 to 4, wherein the rearing pond is provided with an indoor circulating water system.
CN202210703253.XA 2022-06-21 2022-06-21 Method for cultivating Micropterus salmoides twice gonadal maturation within one year Pending CN114831060A (en)

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CN111758620A (en) * 2020-07-20 2020-10-13 淮阴师范学院 Out-of-season breeding method for micropterus salmoides
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CN116941551B (en) * 2023-08-25 2023-12-29 中国科学院水生生物研究所 Temperature treatment method for inducing annual maturation of female carp of yellow river

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