CN105432513B - A kind of method of She Minnow artificial induced spawnings - Google Patents

A kind of method of She Minnow artificial induced spawnings Download PDF

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Publication number
CN105432513B
CN105432513B CN201510703792.3A CN201510703792A CN105432513B CN 105432513 B CN105432513 B CN 105432513B CN 201510703792 A CN201510703792 A CN 201510703792A CN 105432513 B CN105432513 B CN 105432513B
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China
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minnow
parent
spawning
female
parturition
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Chinese (zh)
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CN105432513A (en
Inventor
张云龙
樊启学
吴巧婉
胡伟华
汪帆
杨瑞斌
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Huazhong Agricultural University
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Huazhong Agricultural University
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00—Culture of aquatic animals
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish

Abstract

The invention discloses a kind of method of She Minnow artificial induced spawnings, steps：A, it after ripe She Minnow parents being caught water outlet, is put into indoor spawning pond；B, hasten parturition beginning when, She Minnow parents are pulled out, oxytocic drug is injected after She Minnow are fixed with hand, using pectoral fin base portion inject；C, liquid of hastening parturition is injected per tail female parent, male parent injects liquid of hastening parturition, and the parent for injecting completion is put into spawning pond；D, after reaching Effect time, after pulling raun out spawning pond, body surface is dried with dry towel, ovum is squeezed into dry plastic tub；E, the sperm of fish is obtained, the spermary of milter is removed, is put on filter paper and removes moisture, be put into mortar grind later；F, it using artificial dry fertilization, is fertilized with physiological saline, female-male proportion 10:1.Easy to implement the method, easy to operate, spawning effect is good, effectively solves the problems, such as She Minnow artificial induced spawnings, and Shi She Minnow spawning rates can reach 85% or so, more than 80% fertilization rate reached to.

Description

A kind of method of She Minnow artificial induced spawnings
Technical field
The present invention relates to aquaculture field more particularly to a kind of induced spawning methods of She Minnow, it belongs to suitable for She Minnow The artificial propagation of other kind of fish.
Background technology
She Minnow (Saurogobio dabryi) are under the jurisdiction of Cypriniformes, Cyprinidae, Minnow subfamily, She Minnow belong to, and are a kind of small-sized economy Fish, it is widely distributed in China, it is to the south up to each water system of the Zhujiang River is distributed with She Minnow from Heilungkiang.She Minnow are the small-sized fish of middle lower floor Class, using aquatic insect and Copepods as Major Foods.She Minnow mating periods are the 3-5 months, and ovum has microviscosity, ovum diameter 1.0-1.1mm. She Minnow dressing percentages are high, pierce small between flesh, and meat fertilizer is tender, and delicious flavour, nutritive value is higher, and the market price is high, demand is big.She Minnow As local name spy breed variety, the exploitation of , She Minnow industries is paid close attention to by government and scientific and technical research personnel more and more in recent years It is taken seriously.But since catching intensity is excessive, the supply of , She Minnow can not meet market demand due to environmental pollution etc.. Therefore the artificial breeding of , She Minnow is imperative, but the method that existing document and patent nothing are related to She Minnow artificial induced spawnings, this Problem can not solve to limit the development of She Minnow aquacultures.Compared with other fish the mating period of , She Minnow relatively early, breeding Water temperature is relatively low, this causes the Effect time of its artificial induced spawning relatively long.We are according to the particularity of She Minnow, by multiple examination It tests and gropes, provide the induced spawning method of a set of She Minnow, the development of Wei She Minnow artificial breeding industry lays the foundation.
Invention content
It is to be the provision of a kind of side of She Minnow artificial induced spawnings the purpose of the present invention is being to overcome the shortage of prior art Method, easy to implement the method, easy to operate, spawning effect is good, effectively solves the problems, such as She Minnow artificial induced spawnings, Shi She Minnow spawning rates can Reach 85% or so, more than 80% fertilization rate reached to.
In order to realize above-mentioned purpose, the present invention uses following technical measures：
A kind of method of She Minnow artificial induced spawnings, step are：
A, it after ripe She Minnow parents being caught water outlet, is put into indoor spawning pond and temporarily supports adaptation 2-4h.
B, hasten parturition beginning when, She Minnow parents are pulled out, oxytocic drug are injected after gently She Minnow are fixed with hand, using pectoral fin Base portion is injected, and injection angles, in 45° angle or so, enter needle 1.0cm or so, needle injection with fish body；
C, per tail female parent, injection 0.2ml hastens parturition liquid, and male parent injects 0.1ml and hastens parturition liquid, and when injecting Between be later than female parent 2h, the parent for injecting completion is immediately placed in spawning pond, and long-time operation is avoided to cause stress reaction.
D, after reaching Effect time, after pulling raun out spawning pond, body surface is dried with dry towel, ripe ovum is squeezed into dry In dry plastic tub；
E, the sperm of fish is obtained, the spermary of milter is removed, is put on filter paper and removes moisture, be put into mortar grind later Mill.
F, it using artificial dry fertilization, is fertilized with the physiological saline of 0.5% (mass volume ratio).Female-male proportion is 10:1.
The oxytocic drug selects carp pituitary (PG), luliberin analog A2(LHRH-A2) and Malaysia Three kinds of drug combinations of dioxone (DOM), dosage of hastening parturition use carp pituitary (PG) 3-5mg, luteotropin for every kilogram of parent Liberin analog A2(LHRH-A2) 5-8 μ g and Malaysia dioxone (DOM) 2-3mg, milter dosage halves, the selection of specific dosage It is 12-26 DEG C to be hastened parturition according to parent's maturation situation and water temperature (She Minnow suitable for water temperature) suitably adjustment.
Parent's male and female after injection separate, and are placed in two spawning ponds, the micro- stimulation by running water of discontinuity, promote sexual gland into It is ripe.
The injection oxytocic drug is completed using common injector for medical purpose, cuts off syringe needle headgear before injection It is put on again again behind part so that syringe needle exposes 1.0cm, facilitates injection.
Using this method She Minnow spawning rates up to 85% or so, rate of fertilization 80% or so.
The relationship of the different water temperature Yu She Minnow Effect times of table 1
Water temperature (DEG C) Effect time (h)
Compared with prior art, the beneficial effects of the invention are as follows：
She Minnow induced spawning methods provided by the invention, it is easy to operation, avoid excessive operation should caused by She Minnow parents Swash damage, this method spawning effect is good, can improve the spawning rate and rate of fertilization of She Minnow parents, and Wei She Minnow propagate the development of industry artificially It lays a good foundation.Shi She Minnow spawning rates can reach more than 85%, fertilization rate reached to 80% or so.
Specific embodiment
Embodiment 1：
The technological means of the invention realized is specifically described with reference to example.
A kind of method of She Minnow artificial induced spawnings, step are：
A, morning April 8 seine obtains snake Minnow parents, selects 49 tails maternal, 10 tail male parents.Raun weight 40-50g, phase To fish brood amount 200ind/g or so.Parent is temporarily supported in spawning pond, carries out artificial induced spawning at night.
B, evening 21 April 8:, start artificial induced spawning at 00 point.She Minnow parents are pulled out, are noted after gently She Minnow are fixed with hand Oxytocic drug to be penetrated, is injected using pectoral fin base portion, injection angles are in 45° angle or so with fish body, enter needle 1.0cm or so, and a needle is injected, Per tail female parent injection 0.2ml liquids.The oxytocic drug selects carp pituitary (PG), luliberin similar Object A2(LHRH-A2) and three kinds of drug combinations of Malaysia dioxone (DOM), dosage of hastening parturition is every kilogram of parent using PG 5mg, LHRH-A27 μ g, DOM 3mg, the parent for injecting completion are immediately placed in spawning pond, long-time operation are avoided to cause stress reaction. 21 DEG C of spawning pond water temperature.
C, evening 23 April 8:00, start to inject male parent, operating method (step B) is consistent with raun, the male parent per tail This injection 0.1ml liquids, liquid of hastening parturition are identical with raun (step B).
D, afternoon 13 April 9:00, female She Minnow parents start to lay eggs, and 13:30 ovipositions terminate, Effect time 16.5h.
E, after reaching Effect time, after pulling raun out spawning pond, body surface is dried with dry towel, ripe ovum is squeezed into dry In dry plastic tub.
F, the sperm of fish is obtained, the spermary of milter is removed, is put on filter paper and removes moisture, be put into mortar grind later Mill.Using artificial dry fertilization, it is fertilized with 0.5% physiological saline.
Statistics shares 43 tail raun natural labors after the completion of fertilization, and spawning rate 87.8% obtains ovum amount 31000 altogether.
Evening 23 April 9:00 point of inspection rate of fertilization, 500 ovum of stochastical sampling, test under microscope fertilized eggs number are 396 Grain, rate of fertilization 79.2%.
Embodiment 2：
Next year, we expanded the scale of production, and will be advanced to the middle ten days and the last ten days in March mating period, and general effect is good.
A kind of method of She Minnow artificial induced spawnings, step are：
A drew in the net to obtain snake Minnow parents in morning March 20, had chosen 536 tails female parent, 96 tail male parents, the same day 21 altogether:00 Point starts artificial induced spawning.Raun weight 40-50g, with respect to fish brood amount 220ind/g or so.
Due to only 19 DEG C of spawning pond water temperature, we properly increase the agent dose that will hasten parturition B, every kilogram of parent using PG 5mg, LHRH-A28 μ g, DOM 4mg；That night 21:00 start artificial induced spawning, hasten parturition liquid configuration, injection volume, injecting method etc. with Embodiment 1 is identical.
21 days 16 March of C:00 or so , She Minnow female parents start to lay eggs, and 17:00 or so completion, Effect time 19h.Oviposition side Method, Insemination procedures are same as Example 1.
Random sampling counts 472 tail female parent natural labors, spawning rate 88.1%, 330,000, common property ovum, secondary daily inspection rate of fertilization 83.0%.
D natural conditions Xia She Minnow sexal maturity peak about in or so mid-March, parent's fish brood amount, the bosom ovum in this season Amount is higher, therefore compared with Example 1, the spawning rate and rate of fertilization of example 2 are higher, but since water temperature is relatively low, Effect time It is corresponding to extend.

Claims (1)

1. a kind of method of She Minnow artificial induced spawnings, step are：
After ripe She Minnow parents are caught water outlet, it is put into indoor spawning pond and temporarily supports adaptation 2-4h；
Hasten parturition beginning when, She Minnow parents are pulled out, oxytocic drug is injected after She Minnow are fixed with hand, using pectoral fin base portion inject, Injection angles, in 45° angle, enter 1.0 cm of needle, needle injection with fish body；
It injects 0.2 ml per tail female parent to hasten parturition liquid, male parent injects 0.1 ml and hastens parturition liquid, and injection time is later than female Property 2 h of parent, the parent for injecting completion are put into spawning pond；
After reaching Effect time, after pulling raun out spawning pond, body surface is dried with dry towel, ovum is squeezed into dry plastic tub In；
The sperm of fish is obtained, the spermary of milter is removed, is put on filter paper and removes moisture, be put into mortar grind later；
Using artificial dry fertilization, it is fertilized with the physiological saline of 0.5 % mass volume ratios, female-male proportion 10:1；
The oxytocic drug selects carp pituitary, luliberin analog A2It is closed with three kinds of drugs of Malaysia dioxone With dosage of hastening parturition uses carp pituitary 3-5 mg, luliberin analog A for every kilogram of parent25-8 μ g and Malaysia dioxone 2-3 mg, milter dosage halve；
Parent's male and female after the injection separate, and are placed in two spawning ponds, discontinuity stimulation by running water, promote sexual gland into It is ripe.
CN201510703792.3A 2015-10-26 2015-10-26 A kind of method of She Minnow artificial induced spawnings Expired - Fee Related CN105432513B (en)

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CN109548713A (en) * 2018-11-20 2019-04-02 安徽省农业科学院水产研究所 A kind of artificial fecundation method of stick flower fish
CN110178761B (en) * 2019-06-17 2021-10-29 江西省水产科学研究所 Artificial spawning induction method for rhinogobio ventralis

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CN102301972A (en) * 2011-08-05 2012-01-04 句容市句容水库管理所 Method for artificially breeding Plagiognathops microlepis

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KR20120003961U (en) * 2010-11-27 2012-06-07 신경철 Assembly fish spawning box
KR101059515B1 (en) * 2011-05-25 2011-08-26 전라남도 Spawning induction by water temperature stimulus of larimichthys polyactis

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CN101999331A (en) * 2010-11-26 2011-04-06 中国水产科学研究院长江水产研究所 Breeding method of snakehead by artificial insemination
CN102301972A (en) * 2011-08-05 2012-01-04 句容市句容水库管理所 Method for artificially breeding Plagiognathops microlepis

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