CN110178761B - Artificial spawning induction method for rhinogobio ventralis - Google Patents
Artificial spawning induction method for rhinogobio ventralis Download PDFInfo
- Publication number
- CN110178761B CN110178761B CN201910522089.0A CN201910522089A CN110178761B CN 110178761 B CN110178761 B CN 110178761B CN 201910522089 A CN201910522089 A CN 201910522089A CN 110178761 B CN110178761 B CN 110178761B
- Authority
- CN
- China
- Prior art keywords
- rhinogobio
- water
- artificial
- female
- ventralis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241000692809 Rhinogobio ventralis Species 0.000 title claims abstract description 58
- 238000000034 method Methods 0.000 title claims abstract description 45
- 230000006698 induction Effects 0.000 title claims abstract description 18
- 241000692785 Rhinogobio Species 0.000 claims abstract description 38
- 235000013601 eggs Nutrition 0.000 claims abstract description 32
- 241000251468 Actinopterygii Species 0.000 claims abstract description 30
- 230000012447 hatching Effects 0.000 claims abstract description 22
- 239000012528 membrane Substances 0.000 claims abstract description 16
- 230000009027 insemination Effects 0.000 claims abstract description 15
- 238000011534 incubation Methods 0.000 claims abstract description 9
- 238000004088 simulation Methods 0.000 claims abstract description 8
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 5
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 92
- 238000009395 breeding Methods 0.000 claims description 19
- 230000001488 breeding effect Effects 0.000 claims description 19
- 210000000582 semen Anatomy 0.000 claims description 16
- 239000007924 injection Substances 0.000 claims description 15
- 238000002347 injection Methods 0.000 claims description 15
- 210000004379 membrane Anatomy 0.000 claims description 15
- 239000003814 drug Substances 0.000 claims description 14
- 241001125879 Gobio Species 0.000 claims description 12
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 12
- 229910052760 oxygen Inorganic materials 0.000 claims description 12
- 239000001301 oxygen Substances 0.000 claims description 12
- 230000032696 parturition Effects 0.000 claims description 10
- 229940079593 drug Drugs 0.000 claims description 9
- 230000002196 ecbolic effect Effects 0.000 claims description 9
- 238000003756 stirring Methods 0.000 claims description 9
- 230000000694 effects Effects 0.000 claims description 8
- 230000004936 stimulating effect Effects 0.000 claims description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 7
- 210000001015 abdomen Anatomy 0.000 claims description 7
- 239000011259 mixed solution Substances 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- 229910052573 porcelain Inorganic materials 0.000 claims description 6
- 210000002969 egg yolk Anatomy 0.000 claims description 5
- 230000004720 fertilization Effects 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 4
- 239000002352 surface water Substances 0.000 claims description 4
- 210000003746 feather Anatomy 0.000 claims description 3
- 210000001035 gastrointestinal tract Anatomy 0.000 claims description 3
- 230000000384 rearing effect Effects 0.000 claims description 3
- 238000005286 illumination Methods 0.000 claims description 2
- 235000013345 egg yolk Nutrition 0.000 claims 3
- 230000013020 embryo development Effects 0.000 claims 1
- 238000001914 filtration Methods 0.000 claims 1
- 210000000936 intestine Anatomy 0.000 claims 1
- 230000001850 reproductive effect Effects 0.000 abstract description 5
- 210000004681 ovum Anatomy 0.000 abstract description 3
- 238000009360 aquaculture Methods 0.000 abstract description 2
- 244000144974 aquaculture Species 0.000 abstract description 2
- 230000035558 fertility Effects 0.000 abstract description 2
- 238000012136 culture method Methods 0.000 abstract 1
- 238000011161 development Methods 0.000 description 5
- 230000018109 developmental process Effects 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- 210000001325 yolk sac Anatomy 0.000 description 4
- 239000000049 pigment Substances 0.000 description 3
- KEQXNNJHMWSZHK-UHFFFAOYSA-L 1,3,2,4$l^{2}-dioxathiaplumbetane 2,2-dioxide Chemical compound [Pb+2].[O-]S([O-])(=O)=O KEQXNNJHMWSZHK-UHFFFAOYSA-L 0.000 description 2
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 description 2
- 241000252185 Cobitidae Species 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 230000037237 body shape Effects 0.000 description 2
- 210000002257 embryonic structure Anatomy 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 210000004602 germ cell Anatomy 0.000 description 2
- 230000002710 gonadal effect Effects 0.000 description 2
- 230000035800 maturation Effects 0.000 description 2
- 210000001672 ovary Anatomy 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 230000013933 post-embryonic development Effects 0.000 description 2
- 230000027272 reproductive process Effects 0.000 description 2
- 230000000638 stimulation Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000723346 Cinnamomum camphora Species 0.000 description 1
- 241000252210 Cyprinidae Species 0.000 description 1
- 241001284786 Gobiocypris Species 0.000 description 1
- 208000006735 Periostitis Diseases 0.000 description 1
- 241000219000 Populus Species 0.000 description 1
- 241000692790 Rhinogobio typus Species 0.000 description 1
- 210000000436 anus Anatomy 0.000 description 1
- 238000009412 basement excavation Methods 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 238000005266 casting Methods 0.000 description 1
- 210000003555 cloaca Anatomy 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 210000002249 digestive system Anatomy 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000003889 eye drop Substances 0.000 description 1
- 229940012356 eye drops Drugs 0.000 description 1
- 230000026109 gonad development Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 210000000214 mouth Anatomy 0.000 description 1
- 210000003460 periosteum Anatomy 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000009182 swimming Effects 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses an artificial spawning induction method of rhinogobio ventralis, and belongs to the field of aquaculture. The method comprises the steps of rhinogobio parent selection, habitat simulation, artificial spawning induction, artificial insemination, artificial incubation, management after membrane emergence and the like. The method successfully carries out artificial insemination on the gobiocypobius domestically for the first time, breeds gobiocobius fry, and successfully solves the problems that the gobiocobius is insufficient in reproductive ovum maturity, difficult to inseminate and incapable of hatching under artificial conditions. The method is simple and efficient, 8.5 myristolochia gobiocephala eggs produced by artificial insemination are obtained by four groups of parents, the fertility rate is over 80%, the hatchability is over 60%, and 3.9 myristolocephala gobiocephala larva fish is obtained. The method can provide method support for realizing total artificial propagation of the rhinogobio ventralis population damaged by the environment; meanwhile, a large number of gobiocyprisgobiocyprisgobiocypris fry can be cultured, so that the enrichment, releasing and artificial culture of the gobiocyprisgobiocyprisgobiocyprisgobiocypriscus are realized, and the gobiocyprisgobiocyprisgobiocypriscus culture method has great ecological benefit, economic benefit and social benefit.
Description
Technical Field
The invention belongs to the field of aquaculture, and particularly relates to an artificial spawning induction method of rhinogobio ventralis.
Background
Rhinogobio (Rhinogobio typus Bleeker) belongs to Cyprinales, Cyprinidae, gobio subfamily and genus Rhinogobio, and is commonly called as autumn, Populus longnose, eye of Bullychus megalobium, iron rod loach, loach stick and the like. The gobio is a bottom layer fish, is a benthic fish which is fond to inhabit a slow-flow estuary and a bay tuo with high transparency and sediment and crushed stone substrate, generally reaches sexual maturity after 2-3 years of age, spawns on a running water beach, floats eggs and flows along water. However, the natural resources of the rhinogobio ventralis are greatly challenged due to the reasons of hydropower project construction, sand excavation, over-fishing, pollution and the like, and according to the survey data in 2015 and 2016 of this subject group, the weight of Jiangxiangtai, Jian, Xiujiang, camphor tree and Wu city river segment rhinogobio ventralis in 2015 is very low, and is only 2.30%, 1.28%, 1.14%, 0.86% and 0.35%; it has been reported that the proportion of rhinogobio ventralis in the body fluid section of Chongqing is only 2.37%, and the population resource amount is in a descending trend. Therefore, the rhinogobio gobio propagation technology needs to be broken through to achieve the purposes of artificial breeding and fry provision for restoring resources and carrying out propagation and releasing.
However, at present, related research on the gobiocypobios is rare, only a few related research researches on the gobiocobios resource structure are available, and the precedent about the reproduction technology research and the successful reproduction of the gobiocobios is not reported. The main reason is that basic research related to rhinogobio biology and reproduction biology is lacked, and the problems of strong stress, asynchronous gonadal development, low fertilization rate, difficult hatching and seedling emergence and the like are faced when the wild parent is directly caught for reproduction, so that artificial reproduction of the rhinogobio ventralis is never broken through.
Disclosure of Invention
The invention aims to provide an artificial spawning induction method of rhinogobio ventralis, which successfully solves the problems of insufficient maturation of reproductive eggs of the rhinogobio ventralis under artificial conditions, difficult insemination and incapability of hatching. The method uses a habitat simulation method and an artificial spawning and hatching method, can realize the total artificial propagation of the rhinogobio ventralis, and the batch production of the rhinogobio ventralis fries in the propagation and releasing and artificial culture specifications becomes possible.
In order to achieve the purpose, the invention adopts the following technical scheme:
an artificial spawning induction method of rhinogobio ventralis comprises the following steps:
(1) parent selection: selecting a rhinogobio ventralis with the weight of more than 150g, vigorous activity and perfect body surface from a male rhinogobio ventralis parent; selecting a rhinogobio gobio with the weight of more than 200g, vigorous vitality, intact body surface and soft and expanded belly from a female rhinogobio parent;
the method for distinguishing the parents of the female rhinogobio ventralis and the male rhinogobio ventralis comprises the following steps: in the breeding season, the mature male fish is longer than the female fish in body shape, the reproductive process is prominent, and the seminal fluid flows out when the male fish is lightly pressed on the abdomen; the abdomen of the mature female fish is enlarged, the ovary outline is obvious, and the reproductive pore is large and red.
(2) Artificial hastening parturition: comprises 2 steps of habitat simulation and artificial induced spawning;
the method for simulating the habitat comprises the following steps: temporarily breeding the female rhinogobio and the male rhinogobio separately in a temporary breeding water pool, and shading the temporary breeding water pool by using a shading net to keep the water temperature in the temporary breeding water pool stable at 20-23 ℃; stimulating female rhinogobio ventralis with running water at 2:00 a day in the morning for 5h, wherein the running water speed is 0.4-0.6m/s, the flow rate is adjusted to 0.1m/s in the feeding periods of 8:00-9:00 and 16:00-17:00, the running water speed is kept at 0.2m/s in the rest time, the water dissolved oxygen is kept above 8mg/L, the pH is 6.8-8.0, hastening parturition is started after continuously stimulating with the running water for 10 days, and the feeding is stopped 2 days before hastening parturition; male rhinogobio ventralis is not stimulated by running water, the water flow speed in the temporary culture water tank is kept to be 0.2m/s, dissolved oxygen is kept to be more than 8mg/L, the pH value is 6.8-8.0, a sunshade net is used for shading the temporary culture water tank, and the water temperature in the temporary culture water tank is kept to be stable at 20-23 ℃;
furthermore, when the sunshade net is used for shading the temporary rearing water pool, the sunshade of the sunshade net is determined according to the illumination condition, the sunshade time period is 10:00-14:00, and the sunshade net is not covered at night, so that the man-made interference and the sound interference are reduced.
The artificial induced spawning method comprises the following steps: the female rhinogobio parent adopts a two-needle injection method to inject an oxytocic drug, the drug dosage injected by a first needle is 3/4 of the total dosage, the drug dosage injected by a second needle is 1/4 of the total dosage, and the interval between the two-needle injection is 8 hours; the oxytocic dose of the male parent is half of that of the male parent, and the injection is carried out by adopting a one-time injection method, and the injection time is carried out simultaneously with the second needle of the female rhinogobio ventralis parent; the total dosage of the oxytocic is LRH-A injected into female rhinogobio gobio parent fish per kilogram3 6ug、HCG 800IU、DOM 5mg;
Further, the total dose of the induced spawning medicaments is related to the temperature, when the temperature is lower than 20 ℃, the first needle of the female rhinogobio ventralis parent injects 2/3 of the total dose, the second needle injects 1/3 of the total dose, and the total dose of the induced spawning medicaments is LRH-a of each kilogram of the female rhinogobio ventralis parent3 6ug、HCG 800IU、DOM 5mg。
(3) Artificial insemination: after the artificial spawning induction is finished, putting female and male rhinogobio parents together according to the male-female ratio of 2:3, stimulating by running water, wherein the water flow speed is 0.4-0.6m/s, completely shading by using a shading net and keeping quiet; then observing the activity condition of the male and female rhinogobio ventralis, and starting artificial insemination when the male rhinogobio ventralis overtakes the female rhinogobio ventralis and egg grains appear in the water body;
the artificial insemination method comprises the following steps: fishing out the female gobiocypobius parent, wiping water on the body surface of the fish body by using a dry rag, and wiping the fish eyes to squeeze roes into a clean and dry porcelain bowl, wherein the roes have elasticity, are mellow in color and are golden yellow; then fishing out the male gobiocypobius parent, wiping off the body surface water and reproduction holes, then covering the fish eyes to squeeze the semen into another clean and dry porcelain bowl, and then adding 3-5ML 0.65% of normal saline into the semen to obtain a semen normal saline mixed solution, wherein the semen is milk white and is scattered when meeting water; mixing the semen physiological saline solution mixed solution with the eggs according to the weight ratio of 1:50, stirring and uniformly mixing the mixture by using feathers, adding 10ML of clear water while stirring, standing for 2min after uniformly stirring to obtain fertilized eggs, and then pouring the fertilized eggs into a hatching facility for artificial hatching.
(4) Artificial incubation: hatching by adopting a hatching loop, controlling water flow at 0.2-0.4m/s, observing the development condition of fertilized egg embryos by using a microscope, and counting the fertilization rate in the midgut; after the fertilized eggs are deciduated, the water flow is adjusted to be 0.1-0.2 m/s; the time from fertilization to membrane emergence of the gobiocypobius is 14h, and fry breeding can be started after fry can sink to the water bottom; the hatching water is filtered in advance, the water temperature is maintained at 22.5 +/-0.6 ℃, the pH is 6.8-8.0, and the dissolved oxygen is more than 8 mg/L.
Further, the fertilized egg membrane-outlet post-treatment method comprises the following steps: after the fertilized eggs are emerged from the membrane, the yolk seedlings float in an incubation loop, the water flow speed of the loop is adjusted to be 0.1-0.2m/s, the egg membrane and dead eggs are removed, the water quality is kept stable, the water temperature is maintained at 22.5 +/-0.6 ℃, the pH is 6.8-8.0, and the dissolved oxygen is 5-8mg/L, the yolk sac disappears after 3 days, the fry starts to swim smoothly, the water flow is stabilized at 0.2m/s, the fry sinks after 2 days, and the transfer is carried out after the digestive tract is completely developed, so that the fry culture is carried out.
Compared with the prior art, the artificial propagation method of the rhinogobio ventralis has the following beneficial effects:
(1) the invention successfully solves the problems of insufficient maturation of the reproductive ovum of the rhinogobio ventralis under the artificial condition, difficult insemination and incapability of hatching.
(2) The method for artificially inducing the rhinogobio ventralis provided by the invention is simple and efficient, more than 8.5 million gobio ventralis spawns are artificially inseminated once, the fertility rate is more than 85%, the hatchability is more than 60%, and the emergence rate is about 3.9 million tail.
(3) According to the artificial propagation method of the gobiocypobius, the germ cell is deciduated and then incubated into the gobiocobius fry, the growth speed is high, and the relative time of the post-embryonic development is as follows:
(4) the invention successfully carries out artificial spawning induction on the rhinogobio ventralis domestically for the first time, obtains the fry, and has important effect on protecting the wild resources of the rhinogobio ventralis.
(5) The invention successfully promotes the gonadal development of the gobiocypobius gobiocypris by using a habitat simulation method, so that the egg maturity is over 90 percent, and synchronous spawning is realized. Compared with other fish breeding modes, the invention emphasizes management after film emergence, and needs fine management for 5 days after film emergence, so that fry can be transferred for fry breeding after sinking, which is important for improving the success rate of later-stage fry breeding.
Drawings
FIG. 1: the rhinogobio ventralis is born for 11 hours;
FIG. 2: the rhinogobio ventralis is born for 35 hours;
FIG. 3: the rhinogobio is born for 40 hours after the rhinogobio emerges from the membrane;
FIG. 4: the rhinogobio is born for 52 hours after the rhinogobio emerges from the membrane;
FIG. 5: 76h after the rhinogobio is born;
FIG. 6: the rhinogobio is born for 100 hours;
FIG. 7: and (4) leading the gobiocypobius to emerge from the membrane for 124 h.
Detailed Description
The technical process of the present invention is described in detail below by way of examples:
an artificial spawning induction method of rhinogobio ventralis comprises the following steps:
(1) parent selection: selecting a rhinogobio ventralis with the weight of more than 150g, vigorous activity and perfect body surface from a male rhinogobio ventralis parent; selecting a rhinogobio gobio with the weight of more than 200g, vigorous vitality, intact body surface and soft and expanded belly from a female rhinogobio parent;
the method for distinguishing the parents of the rhinogobio ventralis: in the breeding season, the mature male fish is longer than the female fish in body shape, the reproductive process is prominent, and the seminal fluid flows out when the male fish is lightly pressed on the abdomen; the abdomen of the mature female fish is enlarged, the ovary outline is obvious, and the reproductive pore is large and red.
(2) Artificial hastening parturition: comprises 2 steps of habitat simulation and artificial induced spawning;
the method for simulating the habitat comprises the following steps: temporarily breeding the female rhinogobio and the male rhinogobio separately in a temporary breeding water pool, and shading the temporary breeding water pool by using a shading net to keep the water temperature in the temporary breeding water pool stable at 20-23 ℃; stimulating female rhinogobio ventralis with running water at 2:00 a day in the morning for 5h, wherein the running water speed is 0.5m/s, the flow rate is adjusted to 0.1m/s in bait casting periods of 8:00-9:00 and 16:00-17:00, the running water speed is kept at 0.2m/s in the rest time, the dissolved oxygen in water is kept above 8mg/L, the pH value is 6.8-8.0, hastening parturition is started after continuously stimulating with running water for 10 days, and the feed feeding is stopped 2 days before hastening parturition; male rhinogobio ventralis is not stimulated by running water, the water flow speed in the temporary culture water tank is kept to be 0.2m/s, dissolved oxygen is kept to be more than 8mg/L, pH is kept to be 6.8-8.0, the temporary culture water tank is shaded by using a shading net, and the water temperature in the temporary culture water tank is kept to be stable at 20-23 ℃;
the sun-shading time period is 10:00-14:00, and the sun-shading net is not covered at night, so that the man-made interference and the sound interference are reduced.
The artificial induced spawning method comprises the following steps: the female rhinogobio parent adopts a two-needle injection method to inject an oxytocic drug, the drug dosage injected by a first needle is 3/4 of the total dosage, the drug dosage injected by a second needle is 1/4 of the total dosage, and the interval between the two-needle injection is 8 hours; the oxytocic dose of the male parent is half of that of the male parent, and the injection is carried out by adopting a one-time injection method, and the injection time is carried out simultaneously with the second needle of the female rhinogobio ventralis parent; the total dosage of the oxytocic is LRH-A injected into female rhinogobio gobio parent fish per kilogram3 6ug、HCG 800IU、DOM 5mg。
(3) Artificial insemination: after the artificial spawning induction is finished, putting female and male rhinogobio parents together according to the female-male ratio of 2:3, carrying out running water stimulation, wherein the water flow speed is 0.5m/s, then observing the activity condition of the female rhinogobio, and starting artificial insemination when the male rhinogobio knocks the tail of the female rhinogobio and egg grains appear in the water body;
the artificial insemination method comprises the following steps: fishing out the female gobiocypobius parent, wiping water on the body surface of the fish body by using a dry rag, and wiping the fish eyes to squeeze roes into a clean and dry porcelain bowl, wherein the roes have elasticity, are mellow in color and are golden yellow; then fishing out the male gobiocypobius parent, wiping off the body surface water and reproduction holes, then covering the fish eyes to squeeze the semen into another clean and dry porcelain bowl, and then adding 3-5ML 0.65% of normal saline into the semen to obtain a semen normal saline mixed solution, wherein the semen is milk white and is scattered when meeting water; mixing the semen physiological saline solution mixed solution with the eggs according to the weight ratio of 1:50, stirring and uniformly mixing the mixture by using feathers, adding 10ML of clear water while stirring, standing for 2min after uniformly stirring to obtain fertilized eggs, and then pouring the fertilized eggs into a hatching facility for hatching.
(4) Artificial incubation: hatching by adopting a hatching loop, controlling water flow at 0.2-0.4m/s, observing the development condition of fertilized egg embryos by using a microscope, and counting the fertilization rate in the midgut; after the fertilized eggs are deciduated, the water flow is adjusted to be 0.1-0.2m/s, and the fry can be cultivated after being sunk into the water; the hatching water is filtered in advance, the water temperature is maintained at 22.5 +/-0.6 ℃, the pH is 6.8-8.0, and the dissolved oxygen is more than 8 mg/L.
The fertilized egg membrane-emergence post-treatment method comprises the following steps: after fertilized eggs are emerged from membranes, yolk seedlings float in an incubation circular channel, the water flow speed of the circular channel is adjusted to be 0.15m/s, the egg membranes and dead eggs are removed, the water quality is kept stable, the water temperature is maintained to be 22.5 +/-0.6 ℃, the pH value is 6.8-8.0, and the dissolved oxygen is 5-8mg/L, yolk sacs disappear after 3 days, the fish seedlings start to swim smoothly, the water flow is stabilized to be about 0.2m/s, the fish seedlings sink after 2 days, and the development of the digestive tract can be completely transferred for fish seedling cultivation.
Test example:
according to the specific embodiment method, the rhyncobio gobio ventralis parents (male fish 12 tails and female fish 8 tails) are temporarily cultured in a closed circulating water system and are divided into four groups for artificial spawning induction test from 12 days 4 and 25 days 4 and 2019. Before the experiment begins, male and female parents are separately cultured, the gobio propagation habitat is simulated to stimulate the gonad development of the female fish, and the temporary culture condition is shown in the following table 1. The artificial spawning can be induced after 10 days of the habitat simulation stimulation, the artificial spawning situation and the hatching situation are shown in the table 2, and the postembryonic development situation of the rhinogobio ventralis after the periosteum emergence is shown in the tables 1-7.
TABLE 1 rhinogobio habitat simulation
TABLE 2 spawning and hatching conditions
Specific spawning, insemination, hatching and emergence are shown in table 3.
TABLE 3 hastening parturition
As can be seen from Table 3, by adopting the artificial spawning induction method of the invention, the germ cell rate of the rhinogobio ventralis ova is all 85% or more, the hatching rate is all 60% or more, the spawning number of four groups of rhinogobio ventralis is 8.5 thousands, the number of seedlings is 3.97 thousands, and good spawning induction effect is obtained. As shown in FIGS. 1 to 7, eyedrops appear 11h after the rhinogobio ventralis goes out of the membrane, and the alimentary canal begins to appear 35h after the rhinogobio ventralis goes out of the membrane; fin rays appear 40 hours after film emergence, and cloaca, anus and pigment begin to appear; swimming bladder and oral cavity appear 52h after the membrane emerges, and the pigment deepens; after 76h of membrane emergence, the blood starts to swim smoothly, the pigment on the body surface is increased, the jaw appears, and part of yolk sac appears; the lumbar points are obvious 100 hours after the membrane is removed, the digestive system is completely developed, and the yolk sac disappears; after film discharging, the film was smoothly moved for 124h and sunk.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive.
Claims (3)
1. An artificial spawning induction method of rhinogobio ventralis is characterized by comprising the following steps:
(1) parent selection: selecting a rhinogobio ventralis with the weight of more than 150g, vigorous activity and perfect body surface from a male rhinogobio ventralis parent; selecting a rhinogobio gobio with the weight of more than 200g, vigorous vitality, intact body surface and soft and expanded belly from a female rhinogobio parent;
(2) artificial hastening parturition: comprises 2 steps of habitat simulation and artificial induced spawning;
the method for simulating the habitat comprises the following steps: temporarily breeding the female rhinogobio and the male rhinogobio separately in a temporary breeding water pool, and shading the temporary breeding water pool by a shading net to keep the water temperature in the temporary breeding water pool stable at 20-23 ℃; stimulating female rhinogobio ventralis with running water at 2:00 a day in the morning for 5h, wherein the running water speed is 0.4-0.6m/s, the flow rate is adjusted to 0.1m/s in the feeding periods of 8:00-9:00 and 16:00-17:00, the running water speed is kept at 0.2m/s in the rest time, the water dissolved oxygen is kept above 8mg/L, the pH is 6.8-8.0, hastening parturition is started after continuously stimulating with the running water for 10 days, and the feeding is stopped 2 days before hastening parturition; male rhinogobio ventralis is not stimulated by running water, the water flow speed in the temporary culture water tank is kept to be 0.2m/s, dissolved oxygen is kept to be more than 8mg/L, the pH value is 6.8-8.0, a sunshade net is used for shading the temporary culture water tank, and the water temperature in the temporary culture water tank is kept to be stable at 20-23 ℃;
the artificial induced spawning method comprises the following steps: the female rhinogobio parent adopts a two-needle injection method to inject an oxytocic drug, the drug dosage injected by a first needle is 3/4 of the total dosage, the drug dosage injected by a second needle is 1/4 of the total dosage, and the interval between the two-needle injection is 8 hours; the oxytocic dose of the male parent is half of that of the male parent, and the injection is carried out by adopting a one-time injection method, and the injection time is carried out simultaneously with the second needle of the female rhinogobio ventralis parent; the total dosage of the oxytocic is LRH-A injected into female rhinogobio gobio parent fish per kilogram3 6ug、HCG 800IU、DOM 5mg;
The total dose of the induced spawning medicament is related to the temperature, when the temperature is lower than 20 ℃, the total dose of the induced spawning medicament is 2/3 of the total dose injected by the first needle of the female rhinogobio ventralis parent, the total dose of the total dose injected by the second needle of the female rhinogobio ventralis parent is 1/3 of the total dose, and the total dose of the induced spawning medicament is LRH-A of each kilogram of the female rhinogobio ventralis parent3 6ug、HCG 800IU、DOM 5mg;
(3) Artificial insemination: after the artificial spawning induction is finished, putting female and male rhinogobio parents together according to the male-female ratio of 2:3, stimulating by running water, wherein the water flow speed is 0.4-0.6m/s, completely shading by using a shading net and keeping quiet; then observing the activity conditions of the female and male rhinogobio ventralis, and starting artificial insemination when the male rhinogobio ventralis overtakes the female rhinogobio ventralis and egg grains appear in the water body;
the artificial insemination method comprises the following steps: fishing out the female gobiocypobius parent, wiping the body surface water of the fish body by using a dry rag, and wiping the fish eyes to squeeze the eggs into a clean and dry porcelain bowl; then fishing out male rhinogobio ventralis parents, wiping off body surface water and reproduction holes, then covering fish eyes to squeeze semen into another clean and dry porcelain bowl, then adding 3-5ML 0.65% of normal saline into the semen to obtain semen normal saline mixed solution, then mixing the semen normal saline mixed solution with eggs according to the weight ratio of 1:50, stirring and uniformly mixing the mixture by using feathers, adding 10ML of clear water while stirring, standing for 2min after stirring uniformly, and then pouring the mixture into an incubation facility for artificial incubation;
(4) artificial incubation: hatching by adopting a hatching loop, controlling water flow to be 0.2-0.4m/s, observing the embryo development condition of the fertilized eggs by using a microscope, counting the fertilization rate at the midphase of the primitive intestines, and adjusting the water flow to be 0.1-0.2m/s after the fertilized eggs are deciduated; after the fry can sink to the bottom, fry cultivation can be started; filtering hatching water at 22.5 + -0.6 deg.C, pH of 6.8-8.0, and dissolved oxygen above 8 mg/L.
2. The artificial spawning induction method of rhinogobio according to claim 1, characterized in that: in the step (2), the temporary rearing water pool is shaded by using the shading net, the shading requirement of the shading net is determined according to the illumination condition, the shading time period is 10:00-14:00, and the temporary rearing water pool is not covered at night, so that the man-made interference and the sound interference are reduced.
3. The artificial spawning induction method of rhinogobio according to claim 1, characterized in that: the fertilized egg membrane-emergence post-treatment method in the step (4) comprises the following steps: after the egg yolk sprouts emerge from the membrane, the egg yolk sprouts float in an incubation circular channel, the water flow speed of the circular channel is adjusted to be 0.1-0.2m/s, egg membranes and dead eggs are removed, the water quality is kept stable, the water temperature is maintained at 22.5 +/-0.6 ℃, the pH is 6.8-8.0, and the dissolved oxygen is 5-8mg/L, the egg yolk sacs disappear after 3 days, the fry starts to swim smoothly, the water flow is stabilized at 0.2m/s, the fry sinks after 2 days, and the fry is transferred for cultivation after the digestive tract is completely developed.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910522089.0A CN110178761B (en) | 2019-06-17 | 2019-06-17 | Artificial spawning induction method for rhinogobio ventralis |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910522089.0A CN110178761B (en) | 2019-06-17 | 2019-06-17 | Artificial spawning induction method for rhinogobio ventralis |
Publications (2)
Publication Number | Publication Date |
---|---|
CN110178761A CN110178761A (en) | 2019-08-30 |
CN110178761B true CN110178761B (en) | 2021-10-29 |
Family
ID=67722131
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201910522089.0A Active CN110178761B (en) | 2019-06-17 | 2019-06-17 | Artificial spawning induction method for rhinogobio ventralis |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN110178761B (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111316937A (en) * | 2020-04-08 | 2020-06-23 | 上海市水产研究所 | Method for improving sexual gland development efficiency of parabramis guichenoti female parent |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103960188A (en) * | 2014-05-20 | 2014-08-06 | 中国水产科学研究院长江水产研究所 | Artificial spawning induction method for rhinogobio ventralis |
CN104067963A (en) * | 2014-06-19 | 2014-10-01 | 中国长江三峡集团公司中华鲟研究所 | Rhinogobio ventralis manual propagation method |
CN104663547A (en) * | 2015-03-11 | 2015-06-03 | 中国水产科学研究院长江水产研究所 | Percocypris scaled artificial propagation method |
CN105432513A (en) * | 2015-10-26 | 2016-03-30 | 华中农业大学 | Method for artificial propagation of longnose gudgeon |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102165929A (en) * | 2011-06-02 | 2011-08-31 | 广州市先步农业发展有限公司 | Artificial propagation method for sturgeon |
CN104054611B (en) * | 2014-06-19 | 2015-11-18 | 中国长江三峡集团公司中华鲟研究所 | A kind of long Qi Wen Minnow seed cultural method |
CN104221958B (en) * | 2014-08-19 | 2016-04-20 | 中国水产科学研究院长江水产研究所 | A kind of C. guichenoti induced spawning method |
CN105284676B (en) * | 2015-09-24 | 2018-03-02 | 中国水产科学研究院长江水产研究所 | A kind of artificial culturing method of long Qi Wen Minnow parent populations |
CN105766732A (en) * | 2016-03-30 | 2016-07-20 | 邵侠 | Artificial reproduction method of mandarin fish |
-
2019
- 2019-06-17 CN CN201910522089.0A patent/CN110178761B/en active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103960188A (en) * | 2014-05-20 | 2014-08-06 | 中国水产科学研究院长江水产研究所 | Artificial spawning induction method for rhinogobio ventralis |
CN104067963A (en) * | 2014-06-19 | 2014-10-01 | 中国长江三峡集团公司中华鲟研究所 | Rhinogobio ventralis manual propagation method |
CN104663547A (en) * | 2015-03-11 | 2015-06-03 | 中国水产科学研究院长江水产研究所 | Percocypris scaled artificial propagation method |
CN105432513A (en) * | 2015-10-26 | 2016-03-30 | 华中农业大学 | Method for artificial propagation of longnose gudgeon |
Non-Patent Citations (2)
Title |
---|
"长江上游长鳍吻鮈繁殖生物学研究";姚建伟;《中国优秀硕士学位论文农业科技辑》;20160215(第2期);第23页 * |
"长鳍吻鮈人工繁育的初步研究";管敏 等;《水产科学》;20150531;第34卷(第5期);第294-299页第1.1节,第2.3节 * |
Also Published As
Publication number | Publication date |
---|---|
CN110178761A (en) | 2019-08-30 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN100464633C (en) | Erythtoculer ilishaefornis and megajobrama amblycephala crossbreeding method | |
CN105104263A (en) | Manual schizothorax griseus pellegrin breeding method | |
KR101037876B1 (en) | Method for production of artificial seedlings and culture of boleophthalmus pectinirostris | |
CN102077806A (en) | Method for artificial propagation of giant salamanders and cultivation of baby giant salamanders | |
CN104642212A (en) | Artificial breeding method for onychostoma simus | |
CN105850807B (en) | A kind of artificial fecundation method of La Shi Minnow fishes | |
CN113711953B (en) | Propagation and seedling raising method for hippocampus blossoming | |
KR101465586B1 (en) | Method for mass producing artificial seed of Abudefduf vaigiensis | |
CN108617558A (en) | A kind of artificial breeding method of juvenile stichopus | |
CN101647412B (en) | Method for breeding tridentiger trigonocephalus by artificial induced spawning and insemination | |
CN104041457A (en) | Method for artificial reproduction of brachymystax lenok tsinlingensis | |
CN103493767A (en) | Cross breeding method of odontobutis potamophila and odontobutis yaluensis | |
CN103651220A (en) | Barbodes fuxianensis artificial propagation technology | |
CN114651751A (en) | Artificial fry breeding method for yellow lip fish | |
CN110178761B (en) | Artificial spawning induction method for rhinogobio ventralis | |
CN113841641A (en) | Artificial propagation method of gobiocypris rarus | |
CN101743915B (en) | Cultivation method for enhancing physique of weak young Chinese sturgeon | |
CN113317241A (en) | Method for gonadotropic development and artificial induced spawning of parent fish of takifugu hexamaculatus | |
CN107155956B (en) | Sebastes roseus artificial fry cultivation method | |
CN112167118A (en) | Artificial propagation method of zier whitefish | |
CN1391797A (en) | Artificial breeding and cultivating method for erythroculter ilishaeformis | |
CN112472354A (en) | Artificial insemination method for fertilized fish sebastes schlegeli hilgendorf in ying and building | |
CN107173298B (en) | Phoxinus lagowskii breeding farm and phoxinus lagowskii breeding method | |
CN109197715A (en) | A kind of artificial breeding of Penaeus vannamei method | |
Steyn et al. | Notes on the induced reproduction and development of the tigerfish, Hydrocynus vittatus (Characidae), embryos and larvae |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |