CN110178761B - Artificial spawning induction method for rhinogobio ventralis - Google Patents
Artificial spawning induction method for rhinogobio ventralis Download PDFInfo
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses an artificial spawning induction method of rhinogobio ventralis, and belongs to the field of aquaculture. The method comprises the steps of rhinogobio parent selection, habitat simulation, artificial spawning induction, artificial insemination, artificial incubation, management after membrane emergence and the like. The method successfully carries out artificial insemination on the gobiocypobius domestically for the first time, breeds gobiocobius fry, and successfully solves the problems that the gobiocobius is insufficient in reproductive ovum maturity, difficult to inseminate and incapable of hatching under artificial conditions. The method is simple and efficient, 8.5 myristolochia gobiocephala eggs produced by artificial insemination are obtained by four groups of parents, the fertility rate is over 80%, the hatchability is over 60%, and 3.9 myristolocephala gobiocephala larva fish is obtained. The method can provide method support for realizing total artificial propagation of the rhinogobio ventralis population damaged by the environment; meanwhile, a large number of gobiocyprisgobiocyprisgobiocypris fry can be cultured, so that the enrichment, releasing and artificial culture of the gobiocyprisgobiocyprisgobiocyprisgobiocypriscus are realized, and the gobiocyprisgobiocyprisgobiocypriscus culture method has great ecological benefit, economic benefit and social benefit.
Description
Technical Field
The invention belongs to the field of aquaculture, and particularly relates to an artificial spawning induction method of rhinogobio ventralis.
Background
Rhinogobio (Rhinogobio typus Bleeker) belongs to Cyprinales, Cyprinidae, gobio subfamily and genus Rhinogobio, and is commonly called as autumn, Populus longnose, eye of Bullychus megalobium, iron rod loach, loach stick and the like. The gobio is a bottom layer fish, is a benthic fish which is fond to inhabit a slow-flow estuary and a bay tuo with high transparency and sediment and crushed stone substrate, generally reaches sexual maturity after 2-3 years of age, spawns on a running water beach, floats eggs and flows along water. However, the natural resources of the rhinogobio ventralis are greatly challenged due to the reasons of hydropower project construction, sand excavation, over-fishing, pollution and the like, and according to the survey data in 2015 and 2016 of this subject group, the weight of Jiangxiangtai, Jian, Xiujiang, camphor tree and Wu city river segment rhinogobio ventralis in 2015 is very low, and is only 2.30%, 1.28%, 1.14%, 0.86% and 0.35%; it has been reported that the proportion of rhinogobio ventralis in the body fluid section of Chongqing is only 2.37%, and the population resource amount is in a descending trend. Therefore, the rhinogobio gobio propagation technology needs to be broken through to achieve the purposes of artificial breeding and fry provision for restoring resources and carrying out propagation and releasing.
However, at present, related research on the gobiocypobios is rare, only a few related research researches on the gobiocobios resource structure are available, and the precedent about the reproduction technology research and the successful reproduction of the gobiocobios is not reported. The main reason is that basic research related to rhinogobio biology and reproduction biology is lacked, and the problems of strong stress, asynchronous gonadal development, low fertilization rate, difficult hatching and seedling emergence and the like are faced when the wild parent is directly caught for reproduction, so that artificial reproduction of the rhinogobio ventralis is never broken through.
Disclosure of Invention
The invention aims to provide an artificial spawning induction method of rhinogobio ventralis, which successfully solves the problems of insufficient maturation of reproductive eggs of the rhinogobio ventralis under artificial conditions, difficult insemination and incapability of hatching. The method uses a habitat simulation method and an artificial spawning and hatching method, can realize the total artificial propagation of the rhinogobio ventralis, and the batch production of the rhinogobio ventralis fries in the propagation and releasing and artificial culture specifications becomes possible.
In order to achieve the purpose, the invention adopts the following technical scheme:
an artificial spawning induction method of rhinogobio ventralis comprises the following steps:
(1) parent selection: selecting a rhinogobio ventralis with the weight of more than 150g, vigorous activity and perfect body surface from a male rhinogobio ventralis parent; selecting a rhinogobio gobio with the weight of more than 200g, vigorous vitality, intact body surface and soft and expanded belly from a female rhinogobio parent;
the method for distinguishing the parents of the female rhinogobio ventralis and the male rhinogobio ventralis comprises the following steps: in the breeding season, the mature male fish is longer than the female fish in body shape, the reproductive process is prominent, and the seminal fluid flows out when the male fish is lightly pressed on the abdomen; the abdomen of the mature female fish is enlarged, the ovary outline is obvious, and the reproductive pore is large and red.
(2) Artificial hastening parturition: comprises 2 steps of habitat simulation and artificial induced spawning;
the method for simulating the habitat comprises the following steps: temporarily breeding the female rhinogobio and the male rhinogobio separately in a temporary breeding water pool, and shading the temporary breeding water pool by using a shading net to keep the water temperature in the temporary breeding water pool stable at 20-23 ℃; stimulating female rhinogobio ventralis with running water at 2:00 a day in the morning for 5h, wherein the running water speed is 0.4-0.6m/s, the flow rate is adjusted to 0.1m/s in the feeding periods of 8:00-9:00 and 16:00-17:00, the running water speed is kept at 0.2m/s in the rest time, the water dissolved oxygen is kept above 8mg/L, the pH is 6.8-8.0, hastening parturition is started after continuously stimulating with the running water for 10 days, and the feeding is stopped 2 days before hastening parturition; male rhinogobio ventralis is not stimulated by running water, the water flow speed in the temporary culture water tank is kept to be 0.2m/s, dissolved oxygen is kept to be more than 8mg/L, the pH value is 6.8-8.0, a sunshade net is used for shading the temporary culture water tank, and the water temperature in the temporary culture water tank is kept to be stable at 20-23 ℃;
furthermore, when the sunshade net is used for shading the temporary rearing water pool, the sunshade of the sunshade net is determined according to the illumination condition, the sunshade time period is 10:00-14:00, and the sunshade net is not covered at night, so that the man-made interference and the sound interference are reduced.
The artificial induced spawning method comprises the following steps: the female rhinogobio parent adopts a two-needle injection method to inject an oxytocic drug, the drug dosage injected by a first needle is 3/4 of the total dosage, the drug dosage injected by a second needle is 1/4 of the total dosage, and the interval between the two-needle injection is 8 hours; the oxytocic dose of the male parent is half of that of the male parent, and the injection is carried out by adopting a one-time injection method, and the injection time is carried out simultaneously with the second needle of the female rhinogobio ventralis parent; the total dosage of the oxytocic is LRH-A injected into female rhinogobio gobio parent fish per kilogram3 6ug、HCG 800IU、DOM 5mg；
Further, the total dose of the induced spawning medicaments is related to the temperature, when the temperature is lower than 20 ℃, the first needle of the female rhinogobio ventralis parent injects 2/3 of the total dose, the second needle injects 1/3 of the total dose, and the total dose of the induced spawning medicaments is LRH-a of each kilogram of the female rhinogobio ventralis parent3 6ug、HCG 800IU、DOM 5mg。
(3) Artificial insemination: after the artificial spawning induction is finished, putting female and male rhinogobio parents together according to the male-female ratio of 2:3, stimulating by running water, wherein the water flow speed is 0.4-0.6m/s, completely shading by using a shading net and keeping quiet; then observing the activity condition of the male and female rhinogobio ventralis, and starting artificial insemination when the male rhinogobio ventralis overtakes the female rhinogobio ventralis and egg grains appear in the water body;
the artificial insemination method comprises the following steps: fishing out the female gobiocypobius parent, wiping water on the body surface of the fish body by using a dry rag, and wiping the fish eyes to squeeze roes into a clean and dry porcelain bowl, wherein the roes have elasticity, are mellow in color and are golden yellow; then fishing out the male gobiocypobius parent, wiping off the body surface water and reproduction holes, then covering the fish eyes to squeeze the semen into another clean and dry porcelain bowl, and then adding 3-5ML 0.65% of normal saline into the semen to obtain a semen normal saline mixed solution, wherein the semen is milk white and is scattered when meeting water; mixing the semen physiological saline solution mixed solution with the eggs according to the weight ratio of 1:50, stirring and uniformly mixing the mixture by using feathers, adding 10ML of clear water while stirring, standing for 2min after uniformly stirring to obtain fertilized eggs, and then pouring the fertilized eggs into a hatching facility for artificial hatching.
(4) Artificial incubation: hatching by adopting a hatching loop, controlling water flow at 0.2-0.4m/s, observing the development condition of fertilized egg embryos by using a microscope, and counting the fertilization rate in the midgut; after the fertilized eggs are deciduated, the water flow is adjusted to be 0.1-0.2 m/s; the time from fertilization to membrane emergence of the gobiocypobius is 14h, and fry breeding can be started after fry can sink to the water bottom; the hatching water is filtered in advance, the water temperature is maintained at 22.5 +/-0.6 ℃, the pH is 6.8-8.0, and the dissolved oxygen is more than 8 mg/L.
Further, the fertilized egg membrane-outlet post-treatment method comprises the following steps: after the fertilized eggs are emerged from the membrane, the yolk seedlings float in an incubation loop, the water flow speed of the loop is adjusted to be 0.1-0.2m/s, the egg membrane and dead eggs are removed, the water quality is kept stable, the water temperature is maintained at 22.5 +/-0.6 ℃, the pH is 6.8-8.0, and the dissolved oxygen is 5-8mg/L, the yolk sac disappears after 3 days, the fry starts to swim smoothly, the water flow is stabilized at 0.2m/s, the fry sinks after 2 days, and the transfer is carried out after the digestive tract is completely developed, so that the fry culture is carried out.
Compared with the prior art, the artificial propagation method of the rhinogobio ventralis has the following beneficial effects:
(1) the invention successfully solves the problems of insufficient maturation of the reproductive ovum of the rhinogobio ventralis under the artificial condition, difficult insemination and incapability of hatching.
(2) The method for artificially inducing the rhinogobio ventralis provided by the invention is simple and efficient, more than 8.5 million gobio ventralis spawns are artificially inseminated once, the fertility rate is more than 85%, the hatchability is more than 60%, and the emergence rate is about 3.9 million tail.
(3) According to the artificial propagation method of the gobiocypobius, the germ cell is deciduated and then incubated into the gobiocobius fry, the growth speed is high, and the relative time of the post-embryonic development is as follows:
(4) the invention successfully carries out artificial spawning induction on the rhinogobio ventralis domestically for the first time, obtains the fry, and has important effect on protecting the wild resources of the rhinogobio ventralis.
(5) The invention successfully promotes the gonadal development of the gobiocypobius gobiocypris by using a habitat simulation method, so that the egg maturity is over 90 percent, and synchronous spawning is realized. Compared with other fish breeding modes, the invention emphasizes management after film emergence, and needs fine management for 5 days after film emergence, so that fry can be transferred for fry breeding after sinking, which is important for improving the success rate of later-stage fry breeding.
Drawings
FIG. 1: the rhinogobio ventralis is born for 11 hours;
FIG. 2: the rhinogobio ventralis is born for 35 hours;
FIG. 3: the rhinogobio is born for 40 hours after the rhinogobio emerges from the membrane;
FIG. 4: the rhinogobio is born for 52 hours after the rhinogobio emerges from the membrane;
FIG. 5: 76h after the rhinogobio is born;
FIG. 6: the rhinogobio is born for 100 hours;
FIG. 7: and (4) leading the gobiocypobius to emerge from the membrane for 124 h.
Detailed Description
The technical process of the present invention is described in detail below by way of examples:
an artificial spawning induction method of rhinogobio ventralis comprises the following steps:
(1) parent selection: selecting a rhinogobio ventralis with the weight of more than 150g, vigorous activity and perfect body surface from a male rhinogobio ventralis parent; selecting a rhinogobio gobio with the weight of more than 200g, vigorous vitality, intact body surface and soft and expanded belly from a female rhinogobio parent;
the method for distinguishing the parents of the rhinogobio ventralis: in the breeding season, the mature male fish is longer than the female fish in body shape, the reproductive process is prominent, and the seminal fluid flows out when the male fish is lightly pressed on the abdomen; the abdomen of the mature female fish is enlarged, the ovary outline is obvious, and the reproductive pore is large and red.
(2) Artificial hastening parturition: comprises 2 steps of habitat simulation and artificial induced spawning;
the method for simulating the habitat comprises the following steps: temporarily breeding the female rhinogobio and the male rhinogobio separately in a temporary breeding water pool, and shading the temporary breeding water pool by using a shading net to keep the water temperature in the temporary breeding water pool stable at 20-23 ℃; stimulating female rhinogobio ventralis with running water at 2:00 a day in the morning for 5h, wherein the running water speed is 0.5m/s, the flow rate is adjusted to 0.1m/s in bait casting periods of 8:00-9:00 and 16:00-17:00, the running water speed is kept at 0.2m/s in the rest time, the dissolved oxygen in water is kept above 8mg/L, the pH value is 6.8-8.0, hastening parturition is started after continuously stimulating with running water for 10 days, and the feed feeding is stopped 2 days before hastening parturition; male rhinogobio ventralis is not stimulated by running water, the water flow speed in the temporary culture water tank is kept to be 0.2m/s, dissolved oxygen is kept to be more than 8mg/L, pH is kept to be 6.8-8.0, the temporary culture water tank is shaded by using a shading net, and the water temperature in the temporary culture water tank is kept to be stable at 20-23 ℃;
the sun-shading time period is 10:00-14:00, and the sun-shading net is not covered at night, so that the man-made interference and the sound interference are reduced.
The artificial induced spawning method comprises the following steps: the female rhinogobio parent adopts a two-needle injection method to inject an oxytocic drug, the drug dosage injected by a first needle is 3/4 of the total dosage, the drug dosage injected by a second needle is 1/4 of the total dosage, and the interval between the two-needle injection is 8 hours; the oxytocic dose of the male parent is half of that of the male parent, and the injection is carried out by adopting a one-time injection method, and the injection time is carried out simultaneously with the second needle of the female rhinogobio ventralis parent; the total dosage of the oxytocic is LRH-A injected into female rhinogobio gobio parent fish per kilogram3 6ug、HCG 800IU、DOM 5mg。
(3) Artificial insemination: after the artificial spawning induction is finished, putting female and male rhinogobio parents together according to the female-male ratio of 2:3, carrying out running water stimulation, wherein the water flow speed is 0.5m/s, then observing the activity condition of the female rhinogobio, and starting artificial insemination when the male rhinogobio knocks the tail of the female rhinogobio and egg grains appear in the water body;
the artificial insemination method comprises the following steps: fishing out the female gobiocypobius parent, wiping water on the body surface of the fish body by using a dry rag, and wiping the fish eyes to squeeze roes into a clean and dry porcelain bowl, wherein the roes have elasticity, are mellow in color and are golden yellow; then fishing out the male gobiocypobius parent, wiping off the body surface water and reproduction holes, then covering the fish eyes to squeeze the semen into another clean and dry porcelain bowl, and then adding 3-5ML 0.65% of normal saline into the semen to obtain a semen normal saline mixed solution, wherein the semen is milk white and is scattered when meeting water; mixing the semen physiological saline solution mixed solution with the eggs according to the weight ratio of 1:50, stirring and uniformly mixing the mixture by using feathers, adding 10ML of clear water while stirring, standing for 2min after uniformly stirring to obtain fertilized eggs, and then pouring the fertilized eggs into a hatching facility for hatching.
(4) Artificial incubation: hatching by adopting a hatching loop, controlling water flow at 0.2-0.4m/s, observing the development condition of fertilized egg embryos by using a microscope, and counting the fertilization rate in the midgut; after the fertilized eggs are deciduated, the water flow is adjusted to be 0.1-0.2m/s, and the fry can be cultivated after being sunk into the water; the hatching water is filtered in advance, the water temperature is maintained at 22.5 +/-0.6 ℃, the pH is 6.8-8.0, and the dissolved oxygen is more than 8 mg/L.
The fertilized egg membrane-emergence post-treatment method comprises the following steps: after fertilized eggs are emerged from membranes, yolk seedlings float in an incubation circular channel, the water flow speed of the circular channel is adjusted to be 0.15m/s, the egg membranes and dead eggs are removed, the water quality is kept stable, the water temperature is maintained to be 22.5 +/-0.6 ℃, the pH value is 6.8-8.0, and the dissolved oxygen is 5-8mg/L, yolk sacs disappear after 3 days, the fish seedlings start to swim smoothly, the water flow is stabilized to be about 0.2m/s, the fish seedlings sink after 2 days, and the development of the digestive tract can be completely transferred for fish seedling cultivation.
Test example:
according to the specific embodiment method, the rhyncobio gobio ventralis parents (male fish 12 tails and female fish 8 tails) are temporarily cultured in a closed circulating water system and are divided into four groups for artificial spawning induction test from 12 days 4 and 25 days 4 and 2019. Before the experiment begins, male and female parents are separately cultured, the gobio propagation habitat is simulated to stimulate the gonad development of the female fish, and the temporary culture condition is shown in the following table 1. The artificial spawning can be induced after 10 days of the habitat simulation stimulation, the artificial spawning situation and the hatching situation are shown in the table 2, and the postembryonic development situation of the rhinogobio ventralis after the periosteum emergence is shown in the tables 1-7.
TABLE 1 rhinogobio habitat simulation
TABLE 2 spawning and hatching conditions
Specific spawning, insemination, hatching and emergence are shown in table 3.
TABLE 3 hastening parturition
As can be seen from Table 3, by adopting the artificial spawning induction method of the invention, the germ cell rate of the rhinogobio ventralis ova is all 85% or more, the hatching rate is all 60% or more, the spawning number of four groups of rhinogobio ventralis is 8.5 thousands, the number of seedlings is 3.97 thousands, and good spawning induction effect is obtained. As shown in FIGS. 1 to 7, eyedrops appear 11h after the rhinogobio ventralis goes out of the membrane, and the alimentary canal begins to appear 35h after the rhinogobio ventralis goes out of the membrane; fin rays appear 40 hours after film emergence, and cloaca, anus and pigment begin to appear; swimming bladder and oral cavity appear 52h after the membrane emerges, and the pigment deepens; after 76h of membrane emergence, the blood starts to swim smoothly, the pigment on the body surface is increased, the jaw appears, and part of yolk sac appears; the lumbar points are obvious 100 hours after the membrane is removed, the digestive system is completely developed, and the yolk sac disappears; after film discharging, the film was smoothly moved for 124h and sunk.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive.
Claims (3)
1. An artificial spawning induction method of rhinogobio ventralis is characterized by comprising the following steps:
(1) parent selection: selecting a rhinogobio ventralis with the weight of more than 150g, vigorous activity and perfect body surface from a male rhinogobio ventralis parent; selecting a rhinogobio gobio with the weight of more than 200g, vigorous vitality, intact body surface and soft and expanded belly from a female rhinogobio parent;
(2) artificial hastening parturition: comprises 2 steps of habitat simulation and artificial induced spawning;
the method for simulating the habitat comprises the following steps: temporarily breeding the female rhinogobio and the male rhinogobio separately in a temporary breeding water pool, and shading the temporary breeding water pool by a shading net to keep the water temperature in the temporary breeding water pool stable at 20-23 ℃; stimulating female rhinogobio ventralis with running water at 2:00 a day in the morning for 5h, wherein the running water speed is 0.4-0.6m/s, the flow rate is adjusted to 0.1m/s in the feeding periods of 8:00-9:00 and 16:00-17:00, the running water speed is kept at 0.2m/s in the rest time, the water dissolved oxygen is kept above 8mg/L, the pH is 6.8-8.0, hastening parturition is started after continuously stimulating with the running water for 10 days, and the feeding is stopped 2 days before hastening parturition; male rhinogobio ventralis is not stimulated by running water, the water flow speed in the temporary culture water tank is kept to be 0.2m/s, dissolved oxygen is kept to be more than 8mg/L, the pH value is 6.8-8.0, a sunshade net is used for shading the temporary culture water tank, and the water temperature in the temporary culture water tank is kept to be stable at 20-23 ℃;
the artificial induced spawning method comprises the following steps: the female rhinogobio parent adopts a two-needle injection method to inject an oxytocic drug, the drug dosage injected by a first needle is 3/4 of the total dosage, the drug dosage injected by a second needle is 1/4 of the total dosage, and the interval between the two-needle injection is 8 hours; the oxytocic dose of the male parent is half of that of the male parent, and the injection is carried out by adopting a one-time injection method, and the injection time is carried out simultaneously with the second needle of the female rhinogobio ventralis parent; the total dosage of the oxytocic is LRH-A injected into female rhinogobio gobio parent fish per kilogram3 6ug、HCG 800IU、DOM 5mg；
The total dose of the induced spawning medicament is related to the temperature, when the temperature is lower than 20 ℃, the total dose of the induced spawning medicament is 2/3 of the total dose injected by the first needle of the female rhinogobio ventralis parent, the total dose of the total dose injected by the second needle of the female rhinogobio ventralis parent is 1/3 of the total dose, and the total dose of the induced spawning medicament is LRH-A of each kilogram of the female rhinogobio ventralis parent3 6ug、HCG 800IU、DOM 5mg；
(3) Artificial insemination: after the artificial spawning induction is finished, putting female and male rhinogobio parents together according to the male-female ratio of 2:3, stimulating by running water, wherein the water flow speed is 0.4-0.6m/s, completely shading by using a shading net and keeping quiet; then observing the activity conditions of the female and male rhinogobio ventralis, and starting artificial insemination when the male rhinogobio ventralis overtakes the female rhinogobio ventralis and egg grains appear in the water body;
the artificial insemination method comprises the following steps: fishing out the female gobiocypobius parent, wiping the body surface water of the fish body by using a dry rag, and wiping the fish eyes to squeeze the eggs into a clean and dry porcelain bowl; then fishing out male rhinogobio ventralis parents, wiping off body surface water and reproduction holes, then covering fish eyes to squeeze semen into another clean and dry porcelain bowl, then adding 3-5ML 0.65% of normal saline into the semen to obtain semen normal saline mixed solution, then mixing the semen normal saline mixed solution with eggs according to the weight ratio of 1:50, stirring and uniformly mixing the mixture by using feathers, adding 10ML of clear water while stirring, standing for 2min after stirring uniformly, and then pouring the mixture into an incubation facility for artificial incubation;
(4) artificial incubation: hatching by adopting a hatching loop, controlling water flow to be 0.2-0.4m/s, observing the embryo development condition of the fertilized eggs by using a microscope, counting the fertilization rate at the midphase of the primitive intestines, and adjusting the water flow to be 0.1-0.2m/s after the fertilized eggs are deciduated; after the fry can sink to the bottom, fry cultivation can be started; filtering hatching water at 22.5 + -0.6 deg.C, pH of 6.8-8.0, and dissolved oxygen above 8 mg/L.
2. The artificial spawning induction method of rhinogobio according to claim 1, characterized in that: in the step (2), the temporary rearing water pool is shaded by using the shading net, the shading requirement of the shading net is determined according to the illumination condition, the shading time period is 10:00-14:00, and the temporary rearing water pool is not covered at night, so that the man-made interference and the sound interference are reduced.
3. The artificial spawning induction method of rhinogobio according to claim 1, characterized in that: the fertilized egg membrane-emergence post-treatment method in the step (4) comprises the following steps: after the egg yolk sprouts emerge from the membrane, the egg yolk sprouts float in an incubation circular channel, the water flow speed of the circular channel is adjusted to be 0.1-0.2m/s, egg membranes and dead eggs are removed, the water quality is kept stable, the water temperature is maintained at 22.5 +/-0.6 ℃, the pH is 6.8-8.0, and the dissolved oxygen is 5-8mg/L, the egg yolk sacs disappear after 3 days, the fry starts to swim smoothly, the water flow is stabilized at 0.2m/s, the fry sinks after 2 days, and the fry is transferred for cultivation after the digestive tract is completely developed.
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