CN104067963A - Rhinogobio ventralis manual propagation method - Google Patents
Rhinogobio ventralis manual propagation method Download PDFInfo
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Abstract
The invention relates to a rhinogobio ventralis manual propagation technology which specifically includes four steps of domesticating, strengthening propagation, parent fish spawning induction, fertilization incubation and the like. The method breaks through the rhinogobio ventralis manual propagation technology for the first time at home and abroad, successfully solves the technical difficulties of high stress, difficult domesticating and streak gonads in the rhinogobio ventralis domesticating propagation process, and successfully achieves rhinogobio ventralis manual propagation for the first time. In the period from June 27th to May 9th in 2014, spawning induction of rhinogobio ventralis is conducted successfully for five times, about 97 thousand fertilized eggs are obtained, the fertility rate reaches 98.9%, about 30.5 thousand new-born fish fries are obtained, and stock enhancement of the rhinogobio ventralis becomes possible. The method achieves the technical reference and demonstration effects on protection of valuable rare fishes in the upstream of the Yangtze River and has great economical benefit, social benefit and ecological benefit.
Description
Technical field
The invention belongs to the rare Endemic fish protection of Upper Yangtze River field, be specifically related to the artificial propagation techniques of long Qi Wen Minnow.
Background technology
Long Qi Wen Minnow (
rhinogobio ventralis) be commonly called as soil consumption, be subordinate to Cyprinidae, Minnow subfamily, be mainly distributed in the Heavenly Stems and Earthly Branches draining area in Jinsha jiang River, move in water bottom, keep in dark place, be afraid of agitation, happiness cluster, activity at the bottom of the river of how staggered at stone riprap, torrent dangerous shoal.In recent years, due to the impact of water pollution and mankind's activity, the stock number of long Qi Wen Minnow sharply declines, taking river, Yibin section as example, 1998 older Qi Wen Minnow annual catch account for 12.42% of year total catch, and only account for 2.26% of year total catch ratio to 2002 older Qi Wen Minnow annual catch.
At present, mainly concentrate on the aspects such as thing, genecology and stock number investigation for the research of long Qi Wen Minnow, precedent about its artificial propagation techniques research and successful reproduction there is not yet report, main cause is because long Qi Wen Minnow has specific life habit and reproductive biology feature, what domestication existed in reproductive process stress be by force, tame and docile that food is difficult, the technical barrier of sexual gland degeneration, causes its artificial propagation to produce no breakthroughs always.
Summary of the invention
The invention solves stress be strong in long Qi Wen Minnow domestication reproductive process, tame and docile food difficult, the technical barrier that sexual gland is degenerated, transport from wild fish, raise and train, parent population reinforced cultivating, parent population accelerating is hastened parturition, six aspects of fertilization and hatching and parent population postpartum recovery, successfully set up a set of long Qi Wen Minnow artificial propagation techniques system, successfully realize first the artificial propagation of long Qi Wen Minnow at home and abroad, fill up the blank of long Qi Wen Minnow artificial propagation techniques, make the stock enhancement of realizing long Qi Wen Minnow become possibility, the protection of the rare Endemic fish of Upper Yangtze River is possessed skills and used for reference and exemplary role, protection to torrent fish has great importance.
The present invention realizes by following technical scheme:
The artificial fecundation method of a kind of long Qi Wen Minnow comprises raises and train---reinforced cultivating---accelerating is hastened parturition---incubating oosperm
(1) raise and train, described in raise and train step and comprise sterilization, propagate artificially
A, sterilization, gather after wild long Qi Wen Minnow, with salt sterilization 5 ~ 10min of 1%;
B, propagate artificially, the wild long Qi Wen Minnow after sterilization is placed in and contains artificial fish shelter pond and raise and train; Cultivate water is pure and fresh pollution-free, and dissolved oxygen DO is greater than 6mg/L, pH value 7.5 ~ 8.0, and rate of water exchange is 8 ~ 10 times/day; In pond, the flow control of aquaculture water is in 0.5m/s; Taming and dociling food Feeding time is 19:00 ~ 22:00;
(2) reinforced cultivating
Through the raising and train of one to two month, to select body weight and be greater than 150g, body to grow up in the long fin kiss Minnow of 200mm, physique stalwartness be that parent population is concentrated and carry out reinforced cultivating, reinforced cultivating comprises: ecological stimulation by running water, fortification.
A, ecological stimulation by running water, the bait throwing in of 19:00 ~ 22:00 ingest during and daytime be the unhurried current phase, flow control is below 0.1m/s; After 22:00 ~ next day the ingesting of 6:00 to dawn be the torrent phase, flow velocity is increased to 0.3-0.5m/s;
B, fortification is mainly divided into two stages: at the beginning of the first stage is 1 month first arrival next year of December, by in feed supplement add nutrition fortifier and mode meet parent population to thering is the micro-demand of gonad development facilitation, concrete grammar is: adopt the mode of supplementing interpolation nutrition fortifier taking a-starch as adhesive in feed to carry out fortification, daily ration of feeding is the 4%-5% of long Qi Wen Minnow body weight, the one-tenth of additive is grouped into mainly and comprises: yeast cell wall 6-8g/(kg.d), inorganic salt mixt 3-4g/(kg.d), phytase 1-2g/(kg.d) and vitamin C, vitamin E 3-4g/(kg.d), second stage be first arrival next year January next year by the end of March, by the biological feed such as freezing or fresh mussel meat, snail meat of throwing something and feeding, daily ration of feeding is the 4%-5% of parent population body weight, to ensure the nutrition supply during gonad development,
(3) accelerating is hastened parturition
In the time that natural temperature reaches 16 DEG C, the aquaculture water Flow Velocity of parent population, at 0.4 ~ 0.5m/s, to promote the gonad development of long Qi Wen Minnow, is injected the LHRH-A of 1-1.5ug/kg simultaneously to parent population base portion
2hasten parturition in advance; Parent population is being hastened parturition after 7 ~ 10 days in advance, starts formally to hasten parturition, and male and female are arranged in pairs or groups according to 1:1.2 ratio;
During the hastening parturition of parent population, the active situation of observing at any time parent population, when raun oestruses to high water time, wipes the moisture on body surface and gonopore with dry towel, and crowded ovum enters dry connecing in ovum basin; Milter belly is pushed to ejaculation, and when seminal fluid flows out from milter cloaca, water is rushed in the connecing in ovum basin of ovum is housed immediately, stirs gently 1 ~ 2min with feather, makes sperm fully contact fertilization with ovum, forms fertilized egg;
(4) incubating oosperm
Fertilized egg is placed in to taper incubator and hatches, the sterilization of the potassium permanganate with 20ppm in a week before use of taper incubator, and open water inlet switch and rinse; Hatching water temperature is controlled at 16.5-19 DEG C; Adjusting the inflow in taper incubator until fertilized egg is slowly rushed, is long Qi Wen Minnow fry at taper incubator incubating oosperm;
Realize the artificial propagation of long Qi Wen Minnow by above step.
Further, the artificial fecundation method of a kind of long Qi Wen Minnow also comprises long Qi Wen Minnow long-distance transport step, and wild long Qi Wen Minnow adopts transport-container for living fishes to transport as carrying tool, and transport case material is high density polyethylene (HDPE), volume 200 ~ 400L; When transport, in transport water body, add the MS-222 of 50mg/L and the plant polyose of 5 ~ 10ppm, traffic density is 20-25kg/m
3, transport whole process utilizes pure oxygen to continue oxygenation.
Further, the artificial fecundation method of a kind of long Qi Wen Minnow also wraps parent population postpartum recovery step, in the artificial induced spawning process of parent population, artificial extruding fish body, can cause parent population internal injury, simultaneously, injection oxytocic drug also can cause the wound of its pectoral fin base portion, need to carry out postpartum recovery treatment to close its of fish, rehabilitation agent formula is: 30mg Enrofloxacin, the permanent Wei Kang of 3ml, 0.4mg B B-complex, 5mg ATP disodium and 7ml 0.65% sodium chloride composition, adopt injection system that rehabilitation agent is injected in parent population body, each dosage is 3ml/kg, and injection in 2 days once.
Further, raising and train the artificial fish shelter of step in propagating artificially is to utilize the cobblestone of 10 ~ 20kg size to pile, and cobblestone piles " product " font and hides hiding space for long Qi Wen Minnow provides.
Further, above pond, add a cover the facility that shelters from heat or light of being made by sunshade net, for long Qi Wen Minnow provides dim environment.
Further, hasten parturition in step at accelerating, the middle parent population of formally hastening parturition adopts three pin injections to hasten parturition, the half that milter dosage is raun, and concrete grammar is: first pin injection 1 ~ 2ug/kgLHRH-A
2, after 12h, check, extrude ovum or seminal fluid if fail, inject the second pin, ocyodinic compatibility and dosage are 5 ~ 15ug/kg LHRH-A
2+ 500 ~ 1000IU/kg HCG+5 ~ 10mg/kg DOM, checks after 12h, extrudes ovum or seminal fluid if also fail, and injects the 3rd pin, and ocyodinic compatibility and dosage are 20 ~ 30ug/kg LHRH-A
2+ 1500 ~ 2500IU/kg HCG+15 ~ 25mg/kg DOM.
All right, the syringe that the middle employing syringe needle diameter of formally hastening parturition is 0.33mm is injected, and greatly reduces the wound causing because of the injection of hastening parturition.
Advantage of the present invention is:
1, the present invention successfully breaks through the key of long Qi Wen Minnow artificial propagation techniques first at home and abroad, and successfully having solved in long Qi Wen Minnow domestication reproductive process stress be by force, tame and docile the technical barrier of food difficulty, sexual gland degeneration.
2, the present invention has successfully realized the artificial propagation of long Qi Wen Minnow at home and abroad first, from wild fish transport, raise and train, parent population reinforced cultivating, parent population accelerating are hastened parturition, fertilization and hatching and six aspects of parent population postpartum recovery, successfully set up a set of long Qi Wen Minnow artificial propagation techniques system, filled up the blank of long Qi Wen Minnow artificial propagation techniques.
3, the present invention makes the stock enhancement of realizing long Qi Wen Minnow become possibility, and the protection of the rare Endemic fish of Upper Yangtze River is possessed skills and used for reference and exemplary role, and the protection of torrent fish is had great importance.
4, the present invention has great economic benefit, social benefit and ecological benefits.
Brief description of the drawings
Fig. 1 is flow chart of the present invention.
Embodiment
Describe the technology of the present invention method in detail below by embodiment:
As Fig. 1, (1) long Qi Wen Minnow long-distance transport
In May, 2013 ~ 2013 year December, collect the wild long fin kiss Minnow of 1208 tail in river, Yibin, Sichuan Province section.Wild long Qi Wen Minnow adopts transport-container for living fishes to transport as carrying tool, and transport case material is high density polyethylene (HDPE), volume 200 ~ 400L.When transport, to transporting the composite Chinese herbal (rough gentian, plantain seed, radix bupleuri, Radix Angelicae Sinensis, cape jasmine, siberian motherwort, Radix Glycyrrhizae, rheum officinale, the red sage root, the root of herbaceous peony, glossy ganoderma etc. mix medicine) that adds the MS-222 of 50mg/L and the plant polyose of 5 ~ 10ppm (Li Yang " activation ") and 10 ~ 20ppm in water body, after medicine adds, mixing and stirring can fill fish and starts shipment, and traffic density is 20-25kg/m
3, transport whole process utilizes pure oxygen to continue oxygenation, and transportation survival rate reaches 98%;
(2) raise and train
Wild long Qi Wen Minnow is being transported to behind base, with salt sterilization 5 ~ 10min of 1%, then be placed in artificial fish shelter flowing water culture pattern (the flowing water culture pattern of artificial fish shelter flowing water culture pattern optimum selection simulation Jinsha jiang River natural environment of raising and train, also may according to not locality do not manufacture different environment), breeding pool adopts glass cylinder, water source adopts Jinsha river (in the present embodiment, wild long fin kiss Minnow is from Jinsha jiang River), water quality is pure and fresh pollution-free, dissolved oxygen DO is greater than 6mg/L, pH value 7.5 ~ 8.0, rate of water exchange is 8 ~ 10 times/day; The artificial fish shelter of 5 ~ 8 groups of " product " fonts is built in the bottom, glass cylinder pond that the cobblestone that utilizes 10 ~ 20kg size is 3 ~ 6m at diameter, hides hiding space for long Qi Wen Minnow provides; The water (flow) direction of intaking by reducer pipe adjustment, makes it in pond, form certain flow velocity, and in pond, flow velocity size is controlled in 0.5m/s, to meet the demand of long Qi Wen Minnow to current, reduces its inadaptability to environment; Above glass cylinder, add a cover the facility that shelters from heat or light of being made by sunshade net, for long Qi Wen Minnow provides dim environment;
According to the life habit of ingesting the night of long Qi Wen Minnow, it is tamed and dociled food Feeding time and is selected in 19:00 ~ 22:00 at night;
Raise and train in process and adopt the method for " taming and dociling food after first food calling " to tame and docile food, the mussel meat of throwing something and feeding makes its initial feeding, and feeding volume is 1% of long Qi Wen Minnow body weight, adds to gradually 5% according to its situation feeding volume of ingesting; Treating that it ingests to stablize turns food after one week, by mussel meat is rubbed and is mixed to manufactured feed, after drying, throw something and feed, day feeding volume is 1% of long Qi Wen Minnow body weight, and gradually reduce according to the situation of ingesting the amount of biological feed of feeding of mixing, the manufactured feed until it is ingested completely, adds to 5% gradually according to the feeding volume of its situation manufactured feed of ingesting;
These technological means greatly reduce the stress reaction of long Qi Wen Minnow in artificial environment, have significantly improved the survival rate of long Qi Wen Minnow;
(3) reinforced cultivating
On November 30th, 2013 from wild long fin kiss Minnow pick out 270 urosome types large (body weight 150g, more than the long 200mm of body), physique stalwartness as parent population, concentrate and carry out reinforced cultivating on March 20,1 day to 2014 December in 2013 to parent population, and reinforced cultivating mainly comprises: ecological stimulation by running water and fortification;
Ecological stimulation by running water, according to breeding ecology of long Qi Wen Minnow and the Hydrological characteristics in natural propagation habitat, for meeting wild fish gonad development, the demand of flow condition also should appropriateness be regulated and controled the flow velocity of water body and form stable rhythm in this stage, mainly ingest and movable at night propagating under condition long Qi Wen Minnow artificially, therefore, flow velocity regulation and control emphasis was implemented at night, was specially: during bait throwing in is ingested, (20:00-22:00 at night) and daytime are the unhurried current phase, and flow control is below 0.1m/s; After ingesting to dawn (22:00-6:00 next day) be the torrent phase, flow velocity is increased to 0.3-0.5m/s;
The fortification of parent population is mainly divided into two stages: the first stage is on January 1,1 day to 2014 December in 2013, by in feed supplement add nutrition fortifier and mode meet parent population to thering is the micro-demand of gonad development facilitation, concrete grammar is: adopt the mode of supplementing interpolation nutrition fortifier taking a-starch as adhesive in feed to carry out fortification, daily ration of feeding is 5% of long Qi Wen Minnow body weight, the one-tenth of additive is grouped into mainly and comprises: yeast cell wall 6-8g/(kg.d), inorganic salt mixt 3-4g/(kg.d), phytase 1-2g/(kg.d) and vitamin C, vitamin E 3-4g/(kg.d), second stage is on March 20,2 days to 2014 January in 2014, the freezing mussel meat of throwing something and feeding, and daily ration of feeding is 5% of long fin kiss Minnow body weight, to ensure the nutrition supply during gonad development,
(4) accelerating is hastened parturition
The natural propagation water temperature of long Qi Wen Minnow is 16 ~ 19 DEG C, therefore, in the time that water temperature reaches 16 DEG C, the 270 tail parent populations of concentrating reinforced cultivating are checked, and select that build is large, complete, the vigor good wild fish obvious without wound, biological property of body surface carry out antenatal artificial ripening as parent population, male and female are arranged in pairs or groups according to 1:1.2 ratio;
The discriminating conduct of the ripe raun of long Qi Wen Minnow and milter is: milter pectoral fin and above-head have obvious pearl star, touch up stronger harsh feeling, and raun there is no;
The antenatal accelerating of long Qi Wen Minnow parent population carries out in pond base fabric is provided with the flowing water glass cylinder of artificial fish shelter, glass cylinder diameter 3m; Accelerating strengthens inflow during hastening parturition, and ensures that water velocity is at 0.4 ~ 0.5m/s, to promote the gonad development of long Qi Wen Minnow; Mainly utilize artificial synthetic hormone (LHRH-A
2) gonad development of parent population is accelerated the ripening in advance, accelerating adopts the injection system of " disposable, low dosage ", at the LHRH-A of parent population pectoral fin base portion injection 1-1.5ug/kg
2;
Long Qi Wen Minnow parent population is being accelerated the ripening after 7 ~ 10 days in advance, starts formally to hasten parturition, and in glass cylinder, water velocity is at 0.4 ~ 0.5m/s.The middle parent population of formally hastening parturition adopts three pin injections to hasten parturition, the half that milter dosage is raun, and method is: adopt the syringe that syringe needle diameter is 0.33mm to inject, greatly reduce the wound causing because of the injection of hastening parturition; First pin injection 1 ~ 2ug/kgLHRH-A
2, after 12h, check, extrude ovum or seminal fluid if fail, inject the second pin, ocyodinic compatibility and dosage are 5 ~ 15ug/kg LHRH-A
2+ 500 ~ 1000IU/kg HCG+5 ~ 10mg/kg DOM, checks after 12h, extrudes ovum or seminal fluid if also fail, and injects the 3rd pin, and ocyodinic compatibility and dosage are 20 ~ 30ug/kg LHRH-A
2+ 1500 ~ 2500IU/kg HCG+15 ~ 25mg/kg DOM.Generally, milter ejaculation generally needs 1 ~ 2 pin, and raun ovulation need to be injected 3 pins mostly;
During the hastening parturition of long Qi Wen Minnow, the active situation of observing at any time parent population, finds after its ejaculation when extruding milter belly, stops immediately pushing and put it into the glass cylinder that flow velocity is large, after raun lays eggs, again milter belly is pushed to ejaculation; When raun oestruses to high water time, if gently press raun belly, fish-egg can flow out voluntarily, now, live gonopore with hand, raun is mentioned, wrap fish body with dry towel, to avoid fish body to touch, expose the whole belly of raun, to adopt ovum, before crowded ovum, wipe the moisture on body surface and gonopore with dry towel, crowded ovum enters dry connecing in ovum basin.Now, immediately milter is mentioned, wrap fish body with dry towel, touch to avoid fish body, expose the whole belly of raun so that semen collection, because of the sperm volume of the ripe milter of long Qi Wen Minnow few, so in the time that seminal fluid flows out from milter cloaca, water is rushed in the basin that ovum is housed immediately, stirs gently 1 ~ 2min with feather, makes sperm fully contact fertilization with ovum, form fertilized egg, this has realized to a certain extent raun ovulation, milter and has produced the synchronous of essence;
(5) incubating oosperm
It is that the taper incubator of 140L is hatched that fertilized egg is placed in to volume, the sterilization of the potassium permanganate with 20ppm in a week before use of taper incubator, and open water inlet switch and rinse; The same step of water quality requirement (2) in hatching process, hatching water temperature is controlled at 16.5-19 DEG C; Inflow in adjustment taper incubator is until slowly rush fertilized egg.In incubating oosperm process, constantly developmental stage and the development condition of observed and recorded fertilized egg, primitive gut is added up fertilization rate mid-term, adds up the incubation rate of fertilized egg after membrane;
According to the method described above, bu carried out artificial ripening and hastened parturition the wild parent population of 270 tail for eight times Mays 9 27 days to 2014 March in 2014, successfully hasten parturition five times altogether, obtain ripe milter 14 tails, ripe raun 7 tails, obtain altogether more than 9.7 ten thousand, fertilized egg, fertilization rate reaches 98.9% most, obtains newly hatched larvae approximately 3.05 ten thousand tails;
In order to verify other possibility of this artificial propagation techniques, in the situation that other step is identical, following control group test;
On April 4th, 2014 formally hastens parturition to first group, and raun adopts three pin injections, the half that milter dosage is raun.Ocyodinic compatibility and dosage are: raun injection ocyodinic compatibility and dosage are: the first pin: LHRH-A
22 μ g/kg; The second pin: LHRH-A
210 μ g/kg+ HCG1000IU/kg+PG 1mg/kg; The 3rd pin: LHRH-A
220 μ g/kg+ HCG1500IU/kg+PG 1mg/kg.Its result is: under 16.5 ~ 17 DEG C of conditions of water temperature, the needle gage of three injections is 12h, and the effect time of medicine is 12 ~ 14h, and female, male parent population all fails to hasten parturition successfully.
On April 16th, 2014 is carried out artificial induced spawning to second group, and raun adopts three pin injections, the half that milter dosage is raun.Ocyodinic compatibility and dosage are: the first pin: LHRH-A
21 μ g/kg; The second pin: LHRH-A
210 μ g/kg+ HCG1000IU/kg+DOM 5mg/kg; The 3rd pin: LHRH-A
220 μ g/kg+ HCG1500IU/kg+DOM 10mg/kg.The result of hastening parturition is: under 16.7 ~ 17.2 DEG C of conditions of water temperature, the needle gage of three injections is 12h, the effect time of medicine is 12 ~ 14h, result successfully hasten parturition milter 3 tails, raun 1 tail, obtain more than 30000, fertilized egg, when embryonic development to after date in primitive gut is added up fertilization rate, fertilization rate reached 83.2% in batches, under the condition of 16.5 ~ 17.1 DEG C of hatching water temperatures, hatch long Qi Wen Minnow seedling approximately 12000 tails.
(6) parent population postpartum recovery
After hastening parturition at every turn and finishing, to postpartum long Qi Wen Minnow parent population carry out postpartum recovery, at its pectoral fin base portion injection rehabilitation agent, injected dose is 3ml/kg, within 2 days, injects once, injects 3 ~ 5 times, the rehabilitation rate of 21 tail parent populations in postpartum is 100%.
Claims (7)
1. an artificial fecundation method of long fin kiss Minnow, is characterized in that: described method comprises raises and train---reinforced cultivating---accelerating is hastened parturition---incubating oosperm
(1) raise and train, described in raise and train step and comprise sterilization, propagate artificially
A, sterilization, gather after wild long Qi Wen Minnow, with salt sterilization 5 ~ 10min of 1%;
B, propagate artificially, the wild long Qi Wen Minnow after sterilization is placed in and contains artificial fish shelter pond and raise and train; Cultivate water is pure and fresh pollution-free, and dissolved oxygen DO is greater than 6mg/L, pH value 7.5 ~ 8.0, and rate of water exchange is 8 ~ 10 times/day; In pond, the flow control of aquaculture water is in 0.5m/s; Taming and dociling food Feeding time is 19:00 ~ 22:00;
(2) reinforced cultivating
Through the raising and train of one to two month, to select body weight and be greater than 150g, body to grow up in the long fin kiss Minnow of 200mm, physique stalwartness be that parent population is concentrated and carry out reinforced cultivating, reinforced cultivating comprises: ecological stimulation by running water, fortification;
A, ecological stimulation by running water, the bait throwing in of 19:00 ~ 22:00 ingest during and daytime be the unhurried current phase, flow control is below 0.1m/s; After 22:00 ~ next day the ingesting of 6:00 to dawn be the torrent phase, flow velocity is increased to 0.3-0.5m/s;
B, fortification is mainly divided into two stages: at the beginning of the first stage is 1 month first arrival next year of December, by in feed supplement add nutrition fortifier and mode meet parent population to thering is the micro-demand of gonad development facilitation, concrete grammar is: adopt the mode of supplementing interpolation nutrition fortifier taking a-starch as adhesive in feed to carry out fortification, daily ration of feeding is the 4%-5% of long Qi Wen Minnow body weight, the one-tenth of additive is grouped into mainly and comprises: yeast cell wall 6-8g/(kg.d), inorganic salt mixt 3-4g/(kg.d), phytase 1-2g/(kg.d) and vitamin C, vitamin E 3-4g/(kg.d), second stage be first arrival next year January next year by the end of March, by the biological feed such as freezing or fresh mussel meat, snail meat of throwing something and feeding, daily ration of feeding is the 4%-5% of parent population body weight, to ensure the nutrition supply during gonad development,
(3) accelerating is hastened parturition
In the time that natural temperature reaches 16 DEG C, the aquaculture water Flow Velocity of parent population, at 0.4 ~ 0.5m/s, to promote the gonad development of long Qi Wen Minnow, is injected the LHRH-A of 1-1.5ug/kg simultaneously to parent population base portion
2hasten parturition in advance; Parent population is being hastened parturition after 7 ~ 10 days in advance, starts formally to hasten parturition, and male and female are arranged in pairs or groups according to 1:1.2 ratio;
During the hastening parturition of parent population, the active situation of observing at any time parent population, when raun oestruses to high water time, wipes the moisture on body surface and gonopore with dry towel, and crowded ovum enters dry connecing in ovum basin; Milter belly is pushed to ejaculation, and when seminal fluid flows out from milter cloaca, water is rushed in the connecing in ovum basin of ovum is housed immediately, stirs gently 1 ~ 2min with feather, makes sperm fully contact fertilization with ovum, forms fertilized egg;
(4) incubating oosperm
Fertilized egg is placed in to taper incubator and hatches, the sterilization of the potassium permanganate with 20ppm in a week before use of taper incubator, and open water inlet switch and rinse; Hatching water temperature is controlled at 16.5-19 DEG C; Adjusting the inflow in taper incubator until fertilized egg is slowly rushed, is long Qi Wen Minnow fry at taper incubator incubating oosperm;
Realize the artificial propagation of long Qi Wen Minnow by above step.
2. the artificial fecundation method of a kind of long Qi Wen Minnow according to claim 1, it is characterized in that: described method also comprises long Qi Wen Minnow long-distance transport step, wild long Qi Wen Minnow adopts transport-container for living fishes to transport as carrying tool, and transport case material is high density polyethylene (HDPE), volume 200 ~ 400L; When transport, in transport water body, add the MS-222 of 50mg/L and the plant polyose of 5 ~ 10ppm, traffic density is 20-25kg/m
3, transport whole process utilizes pure oxygen to continue oxygenation.
3. the artificial fecundation method of a kind of long Qi Wen Minnow according to claim 1, it is characterized in that: described method is also wrapped parent population postpartum recovery step, in the artificial induced spawning process of parent population, artificial extruding fish body, can cause parent population internal injury, simultaneously, injection oxytocic drug also can cause the wound of its pectoral fin base portion, need to carry out postpartum recovery treatment to close its of fish, rehabilitation agent formula is: 30mg Enrofloxacin, the permanent Wei Kang of 3ml, 0.4mg B B-complex, 5mg ATP disodium and 7ml 0.65% sodium chloride composition, adopt injection system that rehabilitation agent is injected in parent population body, each dosage is 3ml/kg, injection in 2 days once.
4. the artificial fecundation method of a kind of long Qi Wen Minnow according to claim 1, it is characterized in that: described in to raise and train the artificial fish shelter of step in propagating artificially be to utilize the cobblestone of 10 ~ 20kg size to pile, cobblestone piles " product " font and hides hiding space for long Qi Wen Minnow provides.
5. the artificial fecundation method of a kind of long Qi Wen Minnow according to claim 1, is characterized in that: above pond, add a cover the facility that shelters from heat or light of being made by sunshade net, for long Qi Wen Minnow provides dim environment.
6. the artificial fecundation method of a kind of long Qi Wen Minnow according to claim 1, it is characterized in that: hasten parturition in step at accelerating, the middle parent population of formally hastening parturition adopts three pin injections to hasten parturition, the half that milter dosage is raun, and concrete grammar is: first pin injection 1 ~ 2ug/kgLHRH-A
2, after 12h, check, extrude ovum or seminal fluid if fail, inject the second pin, ocyodinic compatibility and dosage are 5 ~ 15ug/kg LHRH-A
2+ 500 ~ 1000IU/kg HCG+5 ~ 10mg/kg DOM, checks after 12h, extrudes ovum or seminal fluid if also fail, and injects the 3rd pin, and ocyodinic compatibility and dosage are 20 ~ 30ug/kg LHRH-A
2+ 1500 ~ 2500IU/kg HCG+15 ~ 25mg/kg DOM.
7. the artificial fecundation method of a kind of long Qi Wen Minnow according to claim 6, is characterized in that: the syringe that the middle employing syringe needle diameter of formally hastening parturition is 0.33mm is injected, and greatly reduces the wound causing because of the injection of hastening parturition.
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| CN104255614A (en) * | 2014-10-13 | 2015-01-07 | 丽江滇江鱼类繁育有限公司 | Artificial propagation method of schizothorax gymnocypris potanini firmispinatus |
| CN105265362A (en) * | 2015-11-16 | 2016-01-27 | 江西省水产科学研究所 | Cross breeding method for improving growth traits of slender mandarinfish |
| CN105284676A (en) * | 2015-09-24 | 2016-02-03 | 中国水产科学研究院长江水产研究所 | Artificial cultivation method for Rhinogobio Ventralis parent fish |
| CN105724288A (en) * | 2016-02-25 | 2016-07-06 | 中国长江三峡集团公司中华鲟研究所 | Indoor breeding method of fry of rhinogobio ventralis sauvage et dabry |
| CN105994039A (en) * | 2016-05-26 | 2016-10-12 | 中国长江三峡集团公司中华鲟研究所 | Intensive culture method for improving rhinogobio ventralis larva culture survival rate |
| CN110178761A (en) * | 2019-06-17 | 2019-08-30 | 江西省水产科学研究所 | A kind of induced spawning method of Wen Minnow |
| CN111316937A (en) * | 2020-04-08 | 2020-06-23 | 上海市水产研究所 | Method for improving sexual gland development efficiency of parabramis guichenoti female parent |
| CN112335581A (en) * | 2020-10-27 | 2021-02-09 | 江苏省淡水水产研究所 | Fertilized egg for gene knockout and overexpression research of channel catfish and preparation method thereof |
| CN115191384A (en) * | 2022-07-08 | 2022-10-18 | 西双版纳云博水产养殖开发有限公司 | Artificial propagation method of diplopod |
| CN115349468A (en) * | 2022-09-29 | 2022-11-18 | 福建省淡水水产研究所 | Total artificial propagation method of gobiocyparidae |
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| CN104255614A (en) * | 2014-10-13 | 2015-01-07 | 丽江滇江鱼类繁育有限公司 | Artificial propagation method of schizothorax gymnocypris potanini firmispinatus |
| CN104255614B (en) * | 2014-10-13 | 2016-03-02 | 丽江滇江鱼类繁育有限公司 | A kind of schizothoracin hard Juniperus formosana Pan naked carp artificial fecundation method |
| CN105284676A (en) * | 2015-09-24 | 2016-02-03 | 中国水产科学研究院长江水产研究所 | Artificial cultivation method for Rhinogobio Ventralis parent fish |
| CN105265362A (en) * | 2015-11-16 | 2016-01-27 | 江西省水产科学研究所 | Cross breeding method for improving growth traits of slender mandarinfish |
| CN105724288A (en) * | 2016-02-25 | 2016-07-06 | 中国长江三峡集团公司中华鲟研究所 | Indoor breeding method of fry of rhinogobio ventralis sauvage et dabry |
| CN105724288B (en) * | 2016-02-25 | 2018-05-11 | 中国长江三峡集团公司中华鲟研究所 | A kind of long Qi Wen Minnow seed indoor culture methods |
| CN105994039A (en) * | 2016-05-26 | 2016-10-12 | 中国长江三峡集团公司中华鲟研究所 | Intensive culture method for improving rhinogobio ventralis larva culture survival rate |
| CN105994039B (en) * | 2016-05-26 | 2018-09-28 | 中国长江三峡集团公司中华鲟研究所 | A kind of intensive culture method improving long Qi Wen Minnow seed rearing survival rates |
| CN110178761A (en) * | 2019-06-17 | 2019-08-30 | 江西省水产科学研究所 | A kind of induced spawning method of Wen Minnow |
| CN110178761B (en) * | 2019-06-17 | 2021-10-29 | 江西省水产科学研究所 | Artificial spawning induction method for rhinogobio ventralis |
| CN111316937A (en) * | 2020-04-08 | 2020-06-23 | 上海市水产研究所 | Method for improving sexual gland development efficiency of parabramis guichenoti female parent |
| CN112335581A (en) * | 2020-10-27 | 2021-02-09 | 江苏省淡水水产研究所 | Fertilized egg for gene knockout and overexpression research of channel catfish and preparation method thereof |
| CN115191384A (en) * | 2022-07-08 | 2022-10-18 | 西双版纳云博水产养殖开发有限公司 | Artificial propagation method of diplopod |
| CN115191384B (en) * | 2022-07-08 | 2023-06-02 | 西双版纳云博水产养殖开发有限公司 | Artificial breeding method for double-hole fish |
| CN115349468A (en) * | 2022-09-29 | 2022-11-18 | 福建省淡水水产研究所 | Total artificial propagation method of gobiocyparidae |
| CN115349468B (en) * | 2022-09-29 | 2024-03-01 | 福建省淡水水产研究所 | Full artificial breeding method for gobiogobiogobioides |
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