CN104067963B - The artificial fecundation method of a kind of long Qi Wen Minnow - Google Patents
The artificial fecundation method of a kind of long Qi Wen Minnow Download PDFInfo
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Abstract
The present invention relates to long Qi Wen Minnow artificial propagation techniques, specifically comprise and raising and train, reinforced cultivating, parent population accelerating is hastened parturition, four steps such as fertilization and hatching, successfully break through the key of long Qi Wen Minnow artificial propagation techniques at home and abroad first, successfully solving stress be strong in long Qi Wen Minnow domestication reproductive process, tame and docile food difficult, the technical barrier that sexual gland is degenerated, and successfully achieve the artificial propagation of long Qi Wen Minnow first, during March 27 to 9 days Mays in 2014 in 2014, successfully hasten parturition for five times long Qi Wen Minnow, obtain fertilized egg more than 9.7 ten thousand grain altogether, fertilization rate reaches 98.9% most, obtain newly hatched larvae about 3.05 ten thousand tail, this makes the stock enhancement realizing long Qi Wen Minnow become possibility, the protection of the rare Endemic fish of Upper Yangtze River is possessed skills and uses for reference and exemplary role, there is great economic benefit, social benefit and ecological benefits.
Description
Technical field
The invention belongs to Upper Yangtze River rare Endemic fish protection field, be specifically related to the artificial propagation techniques of long Qi Wen Minnow.
Background technology
Long Qi Wen Minnow (
rhinogobioventralis) be commonly called as soil consumption, be subordinate to Cyprinidae, Minnow subfamily, be mainly distributed in the Heavenly Stems and Earthly Branches draining area in Jinsha jiang River, move in water bottom, keep in dark place, be afraid of agitation, happiness cluster, how to interlock at stone riprap, movable at the bottom of the river of torrent dangerous shoal.In recent years, due to the impact of water pollution and mankind's activity, the stock number of long Qi Wen Minnow sharply declines, for river, Yibin section, 1998 older Qi Wen Minnow annual catch account for 12.42% of year total catch, and only account for 2.26% of year total catch ratio to 2002 older Qi Wen Minnow annual catch.
At present, research for long Qi Wen Minnow mainly concentrates on the aspects such as thing, genecology and stock number investigation, about the research of its artificial propagation techniques and the precedent of successful reproduction, there is not been reported, main cause is because long Qi Wen Minnow has specific life habit and reproductive biology feature, exist in domestication reproductive process stress by force, tame and docile that food is difficult, the technical barrier of sexual gland degeneration, cause its artificial propagation to produce no breakthroughs always.
Summary of the invention
The invention solves stress be strong in long Qi Wen Minnow domestication reproductive process, tame and docile food difficult, the technical barrier that sexual gland is degenerated, transport from wild fish, raise and train, parent population reinforced cultivating, parent population accelerating is hastened parturition, fertilization and hatching and parent population postpartum recovery six aspects, successfully set up a set of long Qi Wen Minnow artificial propagation techniques system, successfully achieve the artificial propagation of long Qi Wen Minnow at home and abroad first, fill up the blank of long Qi Wen Minnow artificial propagation techniques, the stock enhancement realizing long Qi Wen Minnow is made to become possibility, the protection of the rare Endemic fish of Upper Yangtze River is possessed skills and uses for reference and exemplary role, the protection of torrent fish is had great importance.
The present invention realizes by following technical scheme:
The artificial fecundation method of a kind of long Qi Wen Minnow comprises raises and train---reinforced cultivating----accelerating is hastened parturition---incubating oosperm
(1) raise and train, described in raise and train step and comprise sterilization, propagate artificially
A, sterilization, after gathering wild long Qi Wen Minnow, with the salt sterilization 5 ~ 10min of 1%;
B, to propagate artificially, the wild long Qi Wen Minnow after sterilization is placed in and raises and train containing artificial fish shelter pond; Cultivate water is pure and fresh pollution-free, and dissolved oxygen DO is greater than 6mg/L, pH value 7.5 ~ 8.0, and rate of water exchange is 8 ~ 10 times/day; In pond, the flow control of aquaculture water is in 0.5m/s; Taming and dociling food Feeding time is 19:00 ~ 22:00;
(2) reinforced cultivating
Through the raising and train of one to two month, selecting that body weight is greater than 150g, body is grown up in the long fin kiss Minnow of 200mm, physique stalwartness is that parent population is concentrated and carries out reinforced cultivating, and reinforced cultivating comprises: ecological stimulation by running water, fortification.
A, ecological stimulation by running water, the bait throwing in of 19:00 ~ 22:00 ingest period and daytime is the unhurried current phase, and flow control is at below 0.1m/s; After the ingesting of 22:00 ~ next day 6:00 to dawn be the torrent phase, flow velocity is increased to 0.3-0.5m/s;
B, fortification is mainly divided into two stages: the first stage is at the beginning of 1 month first arrival next year of December, by in feed supplement add nutrition fortifier and mode meet parent population to the micro-demand with gonad development facilitation, concrete grammar is: adopting with alphalise starch is that adhesive supplementary mode of adding nutrition fortifier in feed carries out fortification, daily ration of feeding is the 4%-5% of long Qi Wen Minnow body weight, the one-tenth of nutrition fortifier is grouped into and mainly comprises: yeast cell wall 6-8g/(kgd), inorganic salt mixt 3-4g/(kgd), phytase 1-2g/(kgd) and vitamin C, vitamin E 3-4g/(kgd), second stage be first arrival next year January next year by the end of March, by the biological feed such as freezing or fresh mussel meat, snail meat of throwing something and feeding, daily ration of feeding is the 4%-5% of parent population body weight, with the nutrition supply during ensureing gonad development,
(3) accelerating is hastened parturition
When natural temperature reaches 16 DEG C, the aquaculture water Flow Velocity of parent population, at 0.4 ~ 0.5m/s, to promote the gonad development of long Qi Wen Minnow, injects the LHRH-A of 1-1.5ug/kg simultaneously to parent population base portion
2hasten parturition in advance; Parent population, after hastening parturition in advance 7 ~ 10 days, starts formally to hasten parturition, and male and female are arranged in pairs or groups according to 1:1.2 ratio;
Hastening parturition period parent population, observes the active situation of parent population at any time, when raun oestruses to high water time, wipes the moisture on body surface and gonopore with dry towel, and crowded ovum enters dry to connect in ovum basin; Extruding ejaculation is carried out to milter belly, when seminal fluid flows out from milter cloaca, is rushed in water immediately and the connecing in ovum basin of ovum is housed, stir 1 ~ 2min gently with feather, make sperm fully contact fertilization with ovum, form fertilized egg;
(4) incubating oosperm
Fertilized egg is placed in taper incubator to hatch, the taper incubator one week before use potassium permanganate with 20ppm is sterilized, and opens water inlet switch and rinse; Hatching water temperature controls at 16.5-19 DEG C; Inflow in adjustment taper incubator, until fertilized egg slowly rushed, is long Qi Wen Minnow fry at taper incubator incubating oosperm;
The artificial propagation of long Qi Wen Minnow is realized by above step.
Further, the artificial fecundation method of a kind of long Qi Wen Minnow also comprises long Qi Wen Minnow long-distance transport step, and wild long Qi Wen Minnow adopts transport-container for living fishes to transport as carrying tool, and transport case material is high density polyethylene (HDPE), volume 200 ~ 400L; During transport, in transport water body, add the MS-222 of 50mg/L and the plant polyose of 5 ~ 10ppm, traffic density is 20-25kg/m
3, transport whole process utilizes pure oxygen to continue oxygenation.
Further, the artificial fecundation method also bag parent population postpartum recovery step of a kind of long Qi Wen Minnow, in the artificial induced spawning process of parent population, artificial extruding fish body, parent population internal injury can be caused, simultaneously, injection oxytocic drug also can cause the wound of its pectoral fin base portion, need to carry out postpartum recovery treatment to parent population, rehabilitation agent formula is: permanent Wei Kang, 0.4mg B B-complex of 30mg Enrofloxacin, 3ml, 5mgATP disodium and 7ml0.65% sodium chloride composition, adopt injection system to be injected by rehabilitation agent in parent population body, each dosage is 3ml/kg, and injection in 2 days once.
Further, raise and train step propagate artificially in artificial fish shelter be utilize the cobblestone of 10 ~ 20kg size to pile, cobblestone piles triangle disposition and hides hiding space for long Qi Wen Minnow provides.
Further, above pond, add a cover the facility that shelters from heat or light made by sunshade net, for long Qi Wen Minnow provides dim environment.
Further, hasten parturition in step at accelerating, middle parent population of formally hastening parturition adopts three pin injections to hasten parturition, and milter dosage is the half of raun, and concrete grammar is: first pin injection 1 ~ 2ug/kgLHRH-A
2, check after 12h, extrude ovum or seminal fluid if fail, then inject the second pin, ocyodinic compatibility and dosage are 5 ~ 15ug/kgLHRH-A
2check after+500 ~ 1000IU/kgHCG+5 ~ 10mg/kgDOM, 12h, extrude ovum or seminal fluid if also fail, then inject the 3rd pin, ocyodinic compatibility and dosage are 20 ~ 30ug/kgLHRH-A
2+ 1500 ~ 2500IU/kgHCG+15 ~ 25mg/kgDOM.
All right, middle employing needle diameter of formally hastening parturition is that the syringe of 0.33mm is injected, and greatly reduces because of the wound of injecting and causing of hastening parturition.
Advantage of the present invention is:
1, the present invention successfully breaks through the key of long Qi Wen Minnow artificial propagation techniques first at home and abroad, successfully solve long Qi Wen Minnow to tame in reproductive process stress by force, tame and docile that food is difficult, the technical barrier of sexual gland degeneration.
2, the present invention successfully achieves the artificial propagation of long Qi Wen Minnow at home and abroad first, transport from wild fish, raise and train, parent population reinforced cultivating, parent population accelerating are hastened parturition, fertilization and hatching and parent population postpartum recovery six aspects, successfully set up a set of long Qi Wen Minnow artificial propagation techniques system, fill up the blank of long Qi Wen Minnow artificial propagation techniques.
3, the present invention makes the stock enhancement realizing long Qi Wen Minnow become possibility, possesses skills and uses for reference and exemplary role, have great importance to the protection of torrent fish to the protection of the rare Endemic fish of Upper Yangtze River.
4, the present invention has great economic benefit, social benefit and ecological benefits.
Accompanying drawing explanation
Fig. 1 is flow chart of the present invention.
Embodiment
The technology of the present invention method is described in detail below by embodiment:
As Fig. 1, (1) long Qi Wen Minnow transports for long-distance
In May, 2013 ~ in December, 2013, collect the wild long fin kiss Minnow of 1208 tail in river, Yibin, Sichuan Province section.Wild long Qi Wen Minnow adopts transport-container for living fishes to transport as carrying tool, and transport case material is high density polyethylene (HDPE), volume 200 ~ 400L.During transport, the composite Chinese herbal (mixing such as rough gentian, plantain seed, radix bupleuri, Radix Angelicae Sinensis, cape jasmine, siberian motherwort, Radix Glycyrrhizae, rheum officinale, the red sage root, the root of herbaceous peony, glossy ganoderma medicine) of the MS-222 of 50mg/L and the plant polyose (Li Yang " activation ") of 5 ~ 10ppm and 10 ~ 20ppm is added in transport water body, after medicine adds, mixing and stirring can fill fish and starts shipment, and traffic density is 20-25kg/m
3, transport whole process utilizes pure oxygen to continue oxygenation, and transportation survival rate reaches 98%;
(2) raise and train
Wild long Qi Wen Minnow is after being transported to base, with the salt sterilization 5 ~ 10min of 1%, then be placed in artificial fish shelter flowing water culture pattern and carry out raising and train that (artificial fish shelter flowing water culture pattern preferably simulates the flowing water culture pattern of Jinsha jiang River natural environment, also may according to not locality do not manufacture different environment), breeding pool adopts glass cylinder, water source adopts Jinsha river (in the present embodiment, wild long fin kiss Minnow is from Jinsha jiang River), water quality is pure and fresh pollution-free, dissolved oxygen DO is greater than 6mg/L, pH value 7.5 ~ 8.0, rate of water exchange is 8 ~ 10 times/day; Utilize the cobblestone of 10 ~ 20kg size to be the artificial fish shelter building 5 ~ 8 groups of triangle dispositions bottom the glass cylinder pond of 3 ~ 6m at diameter, hide hiding space for long Qi Wen Minnow provides; By the water (flow) direction of reducer pipe adjustment water inlet, make it in pond, form certain flow velocity, in pond, flow velocity size controls in 0.5m/s, to meet long Qi Wen Minnow to the demand of current, reduces its inadaptability to environment; The facility that shelters from heat or light made by sunshade net is added a cover, for long Qi Wen Minnow provides dim environment above glass cylinder;
According to the life habit that the night of long Qi Wen Minnow ingests, it is tamed and dociled food Feeding time and is selected in 19:00 ~ 22:00 at night;
Raise and train in process and adopt the method for " taming and dociling food after first food calling " to carry out taming and dociling food, mussel meat of namely throwing something and feeding makes its initial feeding, and feeding volume is 1% of long Qi Wen Minnow body weight, adds to 5% gradually according to its situation feeding volume of ingesting; Treat that it ingests to stablize after one week to carry out turning food, namely mix to manufactured feed by mussel meat is rubbed, throw something and feed after drying, day feeding volume is 1% of long Qi Wen Minnow body weight, and the amount of mixing the biological feed of feeding is gradually reduced according to the situation of ingesting, the manufactured feed until it is ingested completely, adds to 5% gradually according to the feeding volume that it ingests situation manufactured feed;
These technological means greatly reduce the stress reaction of long Qi Wen Minnow in artificial environment, significantly improve the survival rate of long Qi Wen Minnow;
(3) reinforced cultivating
On November 30th, 2013 from wild long fin kiss Minnow pick out 270 urosome types comparatively large (long more than the 200mm of body weight 150g, body), physique stalwartness as parent population, concentrate parent population on March 20,1 day to 2014 December in 2013 and carry out reinforced cultivating, reinforced cultivating mainly comprises: ecological stimulation by running water and fortification;
Ecological stimulation by running water, according to the long breeding ecology of Qi Wen Minnow and the Hydrological characteristics in natural propagation habitat, for meeting wild fish gonad development, the demand of flow condition also appropriateness should be regulated and controled to the flow velocity of water body and forms stable rhythm in this stage, mainly to ingest and movable at night propagating under condition long Qi Wen Minnow artificially, therefore, flow velocity regulation and control emphasis implements at night, be specially: bait throwing in ingest period (20:00-22:00 at night) and daytime be the unhurried current phase, flow control is at below 0.1m/s; After ingesting to dawn (22:00-6:00 next day) be the torrent phase, flow velocity is increased to 0.3-0.5m/s;
The fortification of parent population is mainly divided into two stages: the first stage is on January 1,1 day to 2014 December in 2013, by in feed supplement add nutrition fortifier and mode meet parent population to the micro-demand with gonad development facilitation, concrete grammar is: adopting with alphalise starch is that adhesive supplementary mode of adding nutrition fortifier in feed carries out fortification, daily ration of feeding is 5% of long Qi Wen Minnow body weight, the one-tenth of nutrition fortifier is grouped into and mainly comprises: yeast cell wall 6-8g/(kgd), inorganic salt mixt 3-4g/(kgd), phytase 1-2g/(kgd) and vitamin C, vitamin E 3-4g/(kg.d), second stage is on March 20,2 days to 2014 January in 2014, freezing mussel meat of throwing something and feeding, and daily ration of feeding is 5% of long fin kiss Minnow body weight, with the nutrition supply during ensureing gonad development,
(4) accelerating is hastened parturition
The natural propagation water temperature of long Qi Wen Minnow is 16 ~ 19 DEG C, therefore, when water temperature reaches 16 DEG C, 270 tail parent populations of concentrated reinforced cultivating are checked, and select that build is comparatively large, complete, the vigor good wild fish obvious without wound, biological property of body surface carry out antenatal artificial ripening as parent population, male and female are arranged in pairs or groups according to 1:1.2 ratio;
The discriminating conduct of the ripe raun of long Qi Wen Minnow and milter is: milter pectoral fin and above-head have obvious pearl star, touch up stronger harsh feeling, and raun do not have;
The antenatal accelerating of long Qi Wen Minnow parent population is provided with at pond base fabric in the flowing water glass cylinder of artificial fish shelter and carries out, glass cylinder diameter 3m; Accelerating strengthens inflow during hastening parturition, and ensures that water velocity is at 0.4 ~ 0.5m/s, to promote the gonad development of long Qi Wen Minnow; Mainly utilize Prof. Du Yucang hormone (LHRH-A
2) gonad development of parent population is accelerated the ripening in advance, accelerating adopts the injection system of " disposable, low dosage ", at the LHRH-A of parent population pectoral fin base portion injection 1-1.5 μ g/kg
2;
Long Qi Wen Minnow parent population is after accelerating the ripening in advance 7 ~ 10 days, and start formally to hasten parturition, in glass cylinder, water velocity is at 0.4 ~ 0.5m/s.Middle parent population of formally hastening parturition adopts three pin injections to hasten parturition, and milter dosage is the half of raun, and method is: employing needle diameter is that the syringe of 0.33mm is injected, and greatly reduces because of the wound of injecting and causing of hastening parturition; First pin injection 1 ~ 2 μ g/kgLHRH-A
2, check after 12h, extrude ovum or seminal fluid if fail, then inject the second pin, ocyodinic compatibility and dosage are 5 ~ 15 μ g/kgLHRH-A
2check after+500 ~ 1000IU/kgHCG+5 ~ 10mg/kgDOM, 12h, extrude ovum or seminal fluid if also fail, then inject the 3rd pin, ocyodinic compatibility and dosage are 20 ~ 30 μ g/kgLHRH-A
2+ 1500 ~ 2500IU/kgHCG+15 ~ 25mg/kgDOM.Generally, milter ejaculation generally needs 1 ~ 2 pin, and raun ovulation needs injection 3 pin mostly;
Hastening parturition period at long Qi Wen Minnow, observes the active situation of parent population at any time, after extruding milter belly finds its ejaculation, stops immediately extruding and putting it into the large glass cylinder of flow velocity, after raun lays eggs, again carries out extruding ejaculation to milter belly; When raun oestruses to high water time, if light pressure raun belly, fish-egg can flow out voluntarily, now, gonopore is lived with hand, raun is mentioned, wraps fish body with dry towel, to avoid fish body disorderly dynamic, expose the whole belly of raun, to adopt ovum, the moisture on body surface and gonopore wiped by the crowded front dry towel of ovum, and crowded ovum enters dry connecing in ovum basin.Now, immediately milter is mentioned, wrap fish body with dry towel, to avoid fish body disorderly dynamic, expose the whole belly of raun, so that semen collection, because the sperm volume of the ripe milter of long Qi Wen Minnow is few, so when seminal fluid flows out from milter cloaca, being rushed in water is immediately equipped with in the basin of ovum, stirs 1 ~ 2min gently, make sperm fully contact fertilization with ovum with feather, form fertilized egg, this achieves to a certain extent, and raun ovulates, milter produces the synchronous of essence;
(5) incubating oosperm
Fertilized egg being placed in volume is that the taper incubator of 140L is hatched, and the taper incubator one week before use potassium permanganate with 20ppm is sterilized, and opens water inlet switch and rinse; The same step of water quality requirement in hatching process (2), hatching water temperature controls at 16.5-19 DEG C; Inflow in adjustment taper incubator is until slowly rushed fertilized egg.In incubating oosperm process, the developmental stage of continuous observed and recorded fertilized egg and development condition, primitive gut adds up fertilization rate mid-term, adds up the incubation rate of fertilized egg after membrane;
According to the method described above, bu carried out artificial ripening to the wild parent population of 270 tail and hastened parturition for eight times May 9 27 days to 2014 March in 2014, successfully hasten parturition five times altogether, obtain ripe milter 14 tail, ripe raun 7 tail, obtain fertilized egg more than 9.7 ten thousand grain altogether, fertilization rate reaches 98.9% most, obtains newly hatched larvae about 3.05 ten thousand tail;
In order to verify other possibility of this artificial propagation techniques, when other step is identical, following control group test;
On April 4th, 2014 formally hastens parturition to first group, and raun adopts three pin injections, and milter dosage is the half of raun.Ocyodinic compatibility and dosage are: raun injection ocyodinic compatibility and dosage are: the first pin: LHRH-A
22 μ g/kg; Second pin: LHRH-A
210 μ g/kg+HCG1000IU/kg+PG1mg/kg; 3rd pin: LHRH-A
220 μ g/kg+HCG1500IU/kg+PG1mg/kg.Its result is: under water temperature 16.5 ~ 17 DEG C of conditions, and the needle gage of three injections is 12h, and the Effect time of medicine is 12 ~ 14h, and female, male parent population all fails to hasten parturition successfully.
On April 16th, 2014 carries out artificial induced spawning to second group, and raun adopts three pin injections, and milter dosage is the half of raun.Ocyodinic compatibility and dosage are: the first pin: LHRH-A
21 μ g/kg; Second pin: LHRH-A
210 μ g/kg+HCG1000IU/kg+DOM5mg/kg; 3rd pin: LHRH-A
220 μ g/kg+HCG1500IU/kg+DOM10mg/kg.Result of hastening parturition is: under water temperature 16.7 ~ 17.2 DEG C of conditions, the needle gage of three injections is 12h, the Effect time of medicine is 12 ~ 14h, result is successfully hastened parturition milter 3 tail, raun 1 tail, obtain fertilized egg more than 30000 grain, when in embryonic development to primitive gut, after date adds up fertilization rate in batches, fertilization rate reached 83.2%, under the condition of hatching water temperature 16.5 ~ 17.1 DEG C, hatch long Qi Wen Minnow seedling about 12000 tail.
(6) parent population postpartum recovery
After end of at every turn hastening parturition, carry out postpartum recovery to long Qi Wen Minnow parent population in postpartum, at its pectoral fin base portion injection rehabilitation agent, injected dose is 3ml/kg, and once, inject 3 ~ 5 times, the rehabilitation rate of 21 tail parent populations in postpartum is 100% in injection in 2 days.
Claims (7)
1. an artificial fecundation method of long fin kiss Minnow, is characterized in that: described method comprises raises and train---reinforced cultivating----accelerating is hastened parturition---incubating oosperm
(1) raise and train, described in raise and train step and comprise sterilization, propagate artificially
A, sterilization, after gathering wild long Qi Wen Minnow, with the salt sterilization 5 ~ 10min of 1%;
B, to propagate artificially, the wild long Qi Wen Minnow after sterilization is placed in and raises and train containing artificial fish shelter pond; Cultivate water is pure and fresh pollution-free, and dissolved oxygen DO is greater than 6mg/L, pH value 7.5 ~ 8.0, and rate of water exchange is 8 ~ 10 times/day; In pond, the flow control of aquaculture water is in 0.5m/s; Taming and dociling food Feeding time is 19:00 ~ 22:00;
(2) reinforced cultivating
Through the raising and train of one to two month, selecting that body weight is greater than 150g, body is grown up in the long fin kiss Minnow of 200mm, physique stalwartness is that parent population is concentrated and carries out reinforced cultivating, and reinforced cultivating comprises: ecological stimulation by running water, fortification;
A, ecological stimulation by running water, the bait throwing in of 19:00 ~ 22:00 ingest period and daytime is the unhurried current phase, and flow control is at below 0.1m/s; After the ingesting of 22:00 ~ next day 6:00 to this period of dawn be the torrent phase, flow velocity is increased to 0.3-0.5m/s;
B, fortification is mainly divided into two stages: the first stage is at the beginning of 1 month first arrival next year of December, parent population is met to the micro-demand with gonad development facilitation by supplementing the mode of adding nutrition fortifier in feed, concrete grammar is: adopting with alphalise starch is that adhesive supplementary mode of adding nutrition fortifier in feed carries out fortification, daily ration of feeding is the 4%-5% of long Qi Wen Minnow body weight, the one-tenth of nutrition fortifier is grouped into and mainly comprises: yeast cell wall 6-8g/(kgd), inorganic salt mixt 3-4g/(kgd), phytase 1-2g/(kgd) and vitamin C, vitamin E 3-4g/(kgd), second stage be first arrival next year January next year by the end of March, by freezing or fresh mussel meat of throwing something and feeding, snail sarcobiont bait, daily ration of feeding is the 4%-5% of parent population body weight, with the nutrition supply during ensureing gonad development,
(3) accelerating is hastened parturition
When natural temperature reaches 16 DEG C, the aquaculture water Flow Velocity of parent population, at 0.4 ~ 0.5m/s, to promote the gonad development of long Qi Wen Minnow, injects the LHRH-A of 1-1.5 μ g/kg simultaneously to parent population base portion
2hasten parturition in advance; Parent population, after hastening parturition in advance 7 ~ 10 days, starts formally to hasten parturition, and male and female are arranged in pairs or groups according to 1:1.2 ratio;
Hastening parturition period parent population, observes the active situation of parent population at any time, when raun oestruses to high water time, wipes the moisture on body surface and gonopore with dry towel, and crowded ovum enters dry to connect in ovum basin; Extruding ejaculation is carried out to milter belly, when seminal fluid flows out from milter cloaca, is rushed in water immediately and the connecing in ovum basin of ovum is housed, stir 1 ~ 2min gently with feather, make sperm fully contact fertilization with ovum, form fertilized egg;
(4) incubating oosperm
Fertilized egg is placed in taper incubator to hatch, the taper incubator one week before use potassium permanganate with 20ppm is sterilized, and opens water inlet switch and rinse; Hatching water temperature controls at 16.5-19 DEG C; Inflow in adjustment taper incubator, until fertilized egg slowly rushed, is long Qi Wen Minnow fry at taper incubator incubating oosperm;
The artificial propagation of long Qi Wen Minnow is realized by above step.
2. the artificial fecundation method of a kind of long Qi Wen Minnow according to claim 1, it is characterized in that: described method also comprises long Qi Wen Minnow long-distance transport step, wild long Qi Wen Minnow adopts transport-container for living fishes to transport as carrying tool, and transport case material is high density polyethylene (HDPE), volume 200 ~ 400L; During transport, in transport water body, add the MS-222 of 50mg/L and the plant polyose of 5 ~ 10ppm, traffic density is 20-25kg/m
3, transport whole process utilizes pure oxygen to continue oxygenation.
3. the artificial fecundation method of a kind of long Qi Wen Minnow according to claim 1, it is characterized in that: described method is bag parent population postpartum recovery step also, in the artificial induced spawning process of parent population, artificial extruding fish body, parent population internal injury can be caused, simultaneously, injection oxytocic drug also can cause the wound of its pectoral fin base portion, need to carry out postpartum recovery treatment to parent population, rehabilitation agent formula is: 30mg Enrofloxacin, the permanent Wei Kang of 3ml, 0.4mg B B-complex, 5mgATP disodium and 7ml0.65% sodium chloride composition, injection system is adopted to be injected by rehabilitation agent in parent population body, each dosage is 3ml/kg, injection in 2 days once.
4. the artificial fecundation method of a kind of long Qi Wen Minnow according to claim 1, it is characterized in that: described in raise and train step propagate artificially in artificial fish shelter be utilize the cobblestone of 10 ~ 20kg size to pile, cobblestone piles triangle disposition and hides hiding space for long Qi Wen Minnow provides.
5. the artificial fecundation method of a kind of long Qi Wen Minnow according to claim 1, is characterized in that: above pond, add a cover the facility that shelters from heat or light made by sunshade net, for long Qi Wen Minnow provides dim environment.
6. the artificial fecundation method of a kind of long Qi Wen Minnow according to claim 1, it is characterized in that: hasten parturition in step at accelerating, middle parent population of formally hastening parturition adopts three pin injections to hasten parturition, and milter dosage is the half of raun, and concrete grammar is: the first pin injection 1 ~ 2 μ g/kgLHRH-A
2, check after 12h, extrude ovum or seminal fluid if fail, then inject the second pin, ocyodinic compatibility and dosage are 5 ~ 15 μ g/kgLHRH-A
2check after+500 ~ 1000IU/kgHCG+5 ~ 10mg/kgDOM, 12h, extrude ovum or seminal fluid if also fail, then inject the 3rd pin, ocyodinic compatibility and dosage are 20 ~ 30 μ g/kgLHRH-A
2+ 1500 ~ 2500IU/kgHCG+15 ~ 25mg/kgDOM.
7. the artificial fecundation method of a kind of long Qi Wen Minnow according to claim 6, is characterized in that: middle employing needle diameter of formally hastening parturition is that the syringe of 0.33mm is injected, and greatly reduces because of the wound of injecting and causing of hastening parturition.
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| CN104255614B (en) * | 2014-10-13 | 2016-03-02 | 丽江滇江鱼类繁育有限公司 | A kind of schizothoracin hard Juniperus formosana Pan naked carp artificial fecundation method |
| CN105284676B (en) * | 2015-09-24 | 2018-03-02 | 中国水产科学研究院长江水产研究所 | A kind of artificial culturing method of long Qi Wen Minnow parent populations |
| CN105265362A (en) * | 2015-11-16 | 2016-01-27 | 江西省水产科学研究所 | Cross breeding method for improving growth traits of slender mandarinfish |
| CN105724288B (en) * | 2016-02-25 | 2018-05-11 | 中国长江三峡集团公司中华鲟研究所 | A kind of long Qi Wen Minnow seed indoor culture methods |
| CN105994039B (en) * | 2016-05-26 | 2018-09-28 | 中国长江三峡集团公司中华鲟研究所 | A kind of intensive culture method improving long Qi Wen Minnow seed rearing survival rates |
| CN110178761B (en) * | 2019-06-17 | 2021-10-29 | 江西省水产科学研究所 | Artificial spawning induction method for rhinogobio ventralis |
| CN111316937A (en) * | 2020-04-08 | 2020-06-23 | 上海市水产研究所 | Method for improving sexual gland development efficiency of parabramis guichenoti female parent |
| CN112335581A (en) * | 2020-10-27 | 2021-02-09 | 江苏省淡水水产研究所 | Fertilized egg for gene knockout and overexpression research of channel catfish and preparation method thereof |
| CN115191384B (en) * | 2022-07-08 | 2023-06-02 | 西双版纳云博水产养殖开发有限公司 | Artificial breeding method for double-hole fish |
| CN115349468B (en) * | 2022-09-29 | 2024-03-01 | 福建省淡水水产研究所 | Full artificial breeding method for gobiogobiogobioides |
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